LOCUS       DANKMN010000001        93137 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000001 DANKMN010000000
VERSION     DANKMN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..420
                     /locus_tag="PW224_000001"
     CDS             <1..420
                     /locus_tag="PW224_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL2906724.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="PW224_000002"
     CDS             complement(504..1226)
                     /locus_tag="PW224_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2906725.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="PW224_000003"
     CDS             complement(1216..1953)
                     /locus_tag="PW224_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2906726.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="PW224_000004"
     CDS             complement(1965..2957)
                     /locus_tag="PW224_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2906727.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="PW224_000005"
     CDS             complement(2972..3715)
                     /locus_tag="PW224_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2906728.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="PW224_000006"
     CDS             complement(3956..5278)
                     /locus_tag="PW224_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2906729.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="PW224_000007"
     CDS             complement(5265..6230)
                     /locus_tag="PW224_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2906730.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="PW224_000008"
     CDS             complement(6227..7765)
                     /locus_tag="PW224_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL2906731.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="PW224_000009"
     CDS             complement(8181..8468)
                     /locus_tag="PW224_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906732.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="PW224_000010"
     CDS             8602..9270
                     /locus_tag="PW224_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2906733.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="PW224_000011"
     CDS             complement(9561..10301)
                     /locus_tag="PW224_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906734.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="PW224_000012"
     CDS             complement(10431..11378)
                     /locus_tag="PW224_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2906735.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="PW224_000013"
     CDS             complement(11507..12271)
                     /locus_tag="PW224_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2906736.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="PW224_000014"
     CDS             complement(12275..12664)
                     /locus_tag="PW224_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906737.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="PW224_000015"
     CDS             12799..13341
                     /locus_tag="PW224_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL2906738.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="PW224_000016"
     CDS             13489..14121
                     /locus_tag="PW224_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906739.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="PW224_000017"
     CDS             complement(14255..14380)
                     /locus_tag="PW224_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2906740.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="PW224_000018"
     CDS             complement(14514..15407)
                     /locus_tag="PW224_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL2906741.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="PW224_000019"
     CDS             complement(15589..16515)
                     /locus_tag="PW224_000019"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL2906742.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="PW224_000020"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="PW224_000020"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL2906743.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="PW224_000021"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="PW224_000021"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL2906744.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="PW224_000022"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="PW224_000022"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL2906745.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="PW224_000023"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="PW224_000023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2906746.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="PW224_000024"
     CDS             complement(24786..25220)
                     /locus_tag="PW224_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL2906747.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="PW224_000025"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="PW224_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL2906748.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="PW224_000026"
     CDS             complement(26117..26992)
                     /locus_tag="PW224_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL2906749.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="PW224_000027"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="PW224_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL2906750.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="PW224_000028"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="PW224_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL2906751.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="PW224_000029"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="PW224_000029"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL2906752.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="PW224_000030"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="PW224_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL2906753.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="PW224_000031"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="PW224_000031"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL2906754.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="PW224_000032"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="PW224_000032"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL2906755.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="PW224_000033"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="PW224_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL2906756.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="PW224_000034"
     CDS             complement(35443..36210)
                     /locus_tag="PW224_000034"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL2906757.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="PW224_000035"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="PW224_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL2906758.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="PW224_000036"
     CDS             37323..37721
                     /locus_tag="PW224_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2906759.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            38000..38500
                     /locus_tag="PW224_000037"
     CDS             38000..38500
                     /locus_tag="PW224_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2906760.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="PW224_000038"
     CDS             complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="PW224_000038"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2906761.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39286..39513)
                     /locus_tag="PW224_000039"
     CDS             complement(39286..39513)
                     /locus_tag="PW224_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL2906762.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="PW224_000040"
     CDS             complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="PW224_000040"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2906763.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40048..40761)
                     /locus_tag="PW224_000041"
     CDS             complement(40048..40761)
                     /locus_tag="PW224_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL2906764.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40764..41660)
                     /locus_tag="PW224_000042"
     CDS             complement(40764..41660)
                     /locus_tag="PW224_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2906765.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41818..42660)
                     /locus_tag="PW224_000043"
     CDS             complement(41818..42660)
                     /locus_tag="PW224_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2906766.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42792..43445
                     /locus_tag="PW224_000044"
     CDS             42792..43445
                     /locus_tag="PW224_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL2906767.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43508..44035)
                     /locus_tag="PW224_000045"
     CDS             complement(43508..44035)
                     /locus_tag="PW224_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2906768.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44055..45002)
                     /locus_tag="PW224_000046"
     CDS             complement(44055..45002)
                     /locus_tag="PW224_000046"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2906769.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45014..46900)
                     /locus_tag="PW224_000047"
     CDS             complement(45014..46900)
                     /locus_tag="PW224_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2906770.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(47060..49138)
                     /locus_tag="PW224_000048"
     CDS             complement(47060..49138)
                     /locus_tag="PW224_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL2906771.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49151..49426)
                     /locus_tag="PW224_000049"
     CDS             complement(49151..49426)
                     /locus_tag="PW224_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906772.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49788..50243)
                     /locus_tag="PW224_000050"
     CDS             complement(49788..50243)
                     /locus_tag="PW224_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906773.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50397..51605)
                     /locus_tag="PW224_000051"
     CDS             complement(50397..51605)
                     /locus_tag="PW224_000051"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL2906774.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51717..52049)
                     /locus_tag="PW224_000052"
     CDS             complement(51717..52049)
                     /locus_tag="PW224_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2906775.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52197..53072
                     /locus_tag="PW224_000053"
     CDS             52197..53072
                     /locus_tag="PW224_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2906776.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53154..53735)
                     /locus_tag="PW224_000054"
     CDS             complement(53154..53735)
                     /locus_tag="PW224_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL2906777.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53741..55000)
                     /locus_tag="PW224_000055"
     CDS             complement(53741..55000)
                     /locus_tag="PW224_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2906778.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55002..56048)
                     /locus_tag="PW224_000056"
     CDS             complement(55002..56048)
                     /locus_tag="PW224_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906779.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56283..56558)
                     /locus_tag="PW224_000057"
     CDS             complement(56283..56558)
                     /locus_tag="PW224_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL2906780.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56801..58111)
                     /gene="der"
                     /locus_tag="PW224_000058"
     CDS             complement(56801..58111)
                     /gene="der"
                     /locus_tag="PW224_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL2906781.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="PW224_000059"
     CDS             complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="PW224_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL2906782.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59657..60337)
                     /locus_tag="PW224_000060"
     CDS             complement(59657..60337)
                     /locus_tag="PW224_000060"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL2906783.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60386..61021)
                     /locus_tag="PW224_000061"
     CDS             complement(60386..61021)
                     /locus_tag="PW224_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2906784.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61087..62490)
                     /locus_tag="PW224_000062"
     CDS             complement(61087..62490)
                     /locus_tag="PW224_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL2906785.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(62477..63508)
                     /locus_tag="PW224_000063"
     CDS             complement(62477..63508)
                     /locus_tag="PW224_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2906786.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63539..63760
                     /locus_tag="PW224_000064"
     CDS             63539..63760
                     /locus_tag="PW224_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL2906787.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63925..64662)
                     /locus_tag="PW224_000065"
     CDS             complement(63925..64662)
                     /locus_tag="PW224_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL2906788.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64815..66155)
                     /locus_tag="PW224_000066"
     CDS             complement(64815..66155)
                     /locus_tag="PW224_000066"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL2906789.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66392..67915)
                     /locus_tag="PW224_000067"
     CDS             complement(66392..67915)
                     /locus_tag="PW224_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL2906790.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(68293..68904)
                     /locus_tag="PW224_000068"
     CDS             complement(68293..68904)
                     /locus_tag="PW224_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2906791.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(69027..69152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(69234..69950)
                     /locus_tag="PW224_000069"
     CDS             complement(69234..69950)
                     /locus_tag="PW224_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2906792.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="PW224_000070"
     CDS             complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="PW224_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL2906793.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70509..71306)
                     /locus_tag="PW224_000071"
     CDS             complement(70509..71306)
                     /locus_tag="PW224_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL2906794.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(71276..71647)
                     /locus_tag="PW224_000072"
     CDS             complement(71276..71647)
                     /locus_tag="PW224_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2906795.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="PW224_000073"
     CDS             complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="PW224_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL2906796.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(72649..73122)
                     /locus_tag="PW224_000074"
     CDS             complement(72649..73122)
                     /locus_tag="PW224_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2906797.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(73227..74090)
                     /locus_tag="PW224_000075"
     CDS             complement(73227..74090)
                     /locus_tag="PW224_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2906798.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(74286..76385)
                     /locus_tag="PW224_000076"
     CDS             complement(74286..76385)
                     /locus_tag="PW224_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2906799.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(76605..77714)
                     /gene="rpoD"
                     /locus_tag="PW224_000077"
     CDS             complement(76605..77714)
                     /gene="rpoD"
                     /locus_tag="PW224_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL2906800.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(77738..79579)
                     /locus_tag="PW224_000078"
     CDS             complement(77738..79579)
                     /locus_tag="PW224_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2906801.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(79761..82076)
                     /locus_tag="PW224_000079"
     CDS             complement(79761..82076)
                     /locus_tag="PW224_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2906802.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(82261..83121)
                     /locus_tag="PW224_000080"
     CDS             complement(82261..83121)
                     /locus_tag="PW224_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL2906803.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(83180..83701)
                     /locus_tag="PW224_000081"
     CDS             complement(83180..83701)
                     /locus_tag="PW224_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL2906804.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            83979..84740
                     /locus_tag="PW224_000082"
     CDS             83979..84740
                     /locus_tag="PW224_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2906805.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            84766..87357
                     /gene="mprF"
                     /locus_tag="PW224_000083"
     CDS             84766..87357
                     /gene="mprF"
                     /locus_tag="PW224_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2906806.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(87545..88732)
                     /gene="tuf"
                     /locus_tag="PW224_000084"
     CDS             complement(87545..88732)
                     /gene="tuf"
                     /locus_tag="PW224_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2906807.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            89100..89456
                     /locus_tag="PW224_000085"
     CDS             89100..89456
                     /locus_tag="PW224_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2906808.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(89697..90701)
                     /locus_tag="PW224_000086"
     CDS             complement(89697..90701)
                     /locus_tag="PW224_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL2906809.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            90867..91718
                     /locus_tag="PW224_000087"
     CDS             90867..91718
                     /locus_tag="PW224_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2906810.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            91735..91980
                     /locus_tag="PW224_000088"
     CDS             91735..91980
                     /locus_tag="PW224_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906811.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            92167..92871
                     /locus_tag="PW224_000089"
     CDS             92167..92871
                     /locus_tag="PW224_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906812.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        25865 a        19520 c        15980 g        31772 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcta tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37861 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37921 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37981 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    38041 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    38101 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    38161 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    38221 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    38281 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38341 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38401 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38461 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38521 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38581 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38641 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38701 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38761 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38821 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38881 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38941 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    39001 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    39061 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    39121 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    39181 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    39241 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39301 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39361 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39421 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39481 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39541 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39601 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39661 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39721 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39781 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39841 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39901 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39961 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    40021 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    40081 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    40141 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    40201 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    40261 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40321 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40381 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40441 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40501 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40561 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40621 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40681 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40741 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40801 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40861 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40921 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40981 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    41041 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    41101 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    41161 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    41221 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    41281 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41341 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41401 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41461 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41521 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41581 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41641 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41701 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41761 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41821 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41881 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41941 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    42001 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    42061 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    42121 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    42181 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    42241 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42301 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42361 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42421 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42481 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42541 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42601 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42661 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42721 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42781 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42841 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42901 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42961 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    43021 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    43081 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    43141 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    43201 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    43261 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43321 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43381 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43441 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43501 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43561 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43621 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43681 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43741 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43801 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43861 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43921 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43981 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    44041 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    44101 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    44161 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    44221 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    44281 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44341 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44401 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44461 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44521 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44581 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44641 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44701 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44761 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44821 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44881 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44941 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    45001 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    45061 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    45121 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    45181 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    45241 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    45301 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    45361 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    45421 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    45481 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    45541 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    45601 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    45661 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    45721 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    45781 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    45841 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    45901 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    45961 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    46021 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    46081 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    46141 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    46201 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    46261 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    46321 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    46381 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    46441 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    46501 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    46561 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    46621 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    46681 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    46741 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    46801 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    46861 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    46921 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    46981 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    47041 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    47101 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    47161 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    47221 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    47281 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    47341 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    47401 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    47461 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    47521 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    47581 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    47641 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    47701 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    47761 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    47821 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    47881 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    47941 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    48001 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    48061 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    48121 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    48181 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    48241 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    48301 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    48361 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    48421 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    48481 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    48541 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    48601 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    48661 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    48721 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    48781 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    48841 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    48901 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    48961 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    49021 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    49081 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    49141 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    49201 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    49261 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    49321 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    49381 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    49441 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    49501 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    49561 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    49621 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    49681 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    49741 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    49801 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    49861 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
    49921 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
    49981 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
    50041 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
    50101 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
    50161 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
    50221 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
    50281 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
    50341 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
    50401 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
    50461 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
    50521 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
    50581 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
    50641 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
    50701 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
    50761 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
    50821 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
    50881 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
    50941 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
    51001 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
    51061 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
    51121 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
    51181 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
    51241 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
    51301 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
    51361 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
    51421 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
    51481 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
    51541 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
    51601 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
    51661 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
    51721 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
    51781 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
    51841 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
    51901 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
    51961 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
    52021 atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac
    52081 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
    52141 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
    52201 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
    52261 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
    52321 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
    52381 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
    52441 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
    52501 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
    52561 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
    52621 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
    52681 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
    52741 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
    52801 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
    52861 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
    52921 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
    52981 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
    53041 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
    53101 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
    53161 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
    53221 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
    53281 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
    53341 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
    53401 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
    53461 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
    53521 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
    53581 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
    53641 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
    53701 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
    53761 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
    53821 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
    53881 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
    53941 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
    54001 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
    54061 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
    54121 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
    54181 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
    54241 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
    54301 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
    54361 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
    54421 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
    54481 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
    54541 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
    54601 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
    54661 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
    54721 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
    54781 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
    54841 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
    54901 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
    54961 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
    55021 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
    55081 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
    55141 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
    55201 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
    55261 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
    55321 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
    55381 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
    55441 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
    55501 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
    55561 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
    55621 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
    55681 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
    55741 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
    55801 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
    55861 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
    55921 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
    55981 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
    56041 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
    56101 aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac
    56161 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
    56221 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
    56281 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
    56341 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
    56401 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
    56461 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
    56521 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
    56581 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
    56641 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
    56701 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
    56761 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
    56821 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
    56881 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
    56941 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
    57001 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
    57061 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
    57121 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
    57181 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
    57241 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
    57301 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
    57361 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
    57421 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
    57481 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
    57541 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
    57601 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
    57661 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
    57721 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
    57781 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
    57841 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
    57901 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
    57961 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
    58021 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
    58081 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
    58141 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
    58201 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc
    58261 tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt
    58321 tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt
    58381 tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct
    58441 tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc
    58501 actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa
    58561 atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag
    58621 cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt
    58681 tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga
    58741 ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc acttggtttt
    58801 gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg
    58861 acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg
    58921 atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc
    58981 gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg
    59041 cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa
    59101 ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg
    59161 atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa
    59221 cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt actgataacg
    59281 actaaatcta atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt
    59341 gataattctt ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact
    59401 tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt
    59461 acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc
    59521 ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat
    59581 tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt
    59641 catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg
    59701 ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt
    59761 gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg
    59821 tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg
    59881 cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg
    59941 tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag
    60001 caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat
    60061 ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt
    60121 cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg
    60181 aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat
    60241 ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg
    60301 aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa
    60361 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
    60421 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
    60481 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
    60541 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
    60601 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
    60661 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
    60721 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
    60781 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
    60841 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
    60901 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
    60961 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
    61021 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
    61081 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttc cccaatgaag
    61141 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
    61201 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
    61261 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
    61321 tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
    61381 ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata
    61441 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
    61501 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
    61561 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat
    61621 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
    61681 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
    61741 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
    61801 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
    61861 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
    61921 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
    61981 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
    62041 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
    62101 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
    62161 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
    62221 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
    62281 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata
    62341 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
    62401 ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc
    62461 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
    62521 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
    62581 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
    62641 tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
    62701 ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca
    62761 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
    62821 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
    62881 tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc
    62941 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
    63001 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt
    63061 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
    63121 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
    63181 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
    63241 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
    63301 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
    63361 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
    63421 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
    63481 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
    63541 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
    63601 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
    63661 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
    63721 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
    63781 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
    63841 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
    63901 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
    63961 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
    64021 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
    64081 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
    64141 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
    64201 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
    64261 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
    64321 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
    64381 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
    64441 tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc
    64501 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
    64561 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
    64621 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
    64681 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa
    64741 tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt
    64801 ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata aatttccatt
    64861 gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta
    64921 tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg
    64981 cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt
    65041 ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa
    65101 gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat
    65161 gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt
    65221 gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca
    65281 ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact
    65341 tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg
    65401 tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt
    65461 tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga
    65521 acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag
    65581 taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat
    65641 ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct
    65701 tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa
    65761 gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca
    65821 actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc
    65881 cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc
    65941 tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg
    66001 atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct
    66061 gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct
    66121 tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa
    66181 aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc
    66241 ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa
    66301 tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta
    66361 tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca
    66421 cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa
    66481 catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt
    66541 ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa
    66601 attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa
    66661 tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca cttgaagata
    66721 atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca
    66781 taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag
    66841 caccagctaa aaattcagag acaaattcag actctttcat gccataagtg attgctacta
    66901 cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa
    66961 gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg
    67021 attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga
    67081 taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt
    67141 gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta
    67201 atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa
    67261 tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc
    67321 caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta
    67381 cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct acagccatca
    67441 tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc
    67501 cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt
    67561 tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa
    67621 atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta
    67681 gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc
    67741 cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta
    67801 cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca ataataataa
    67861 ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct
    67921 tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag
    67981 agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt
    68041 ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt
    68101 ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt tcacatttgc
    68161 aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac
    68221 gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga aatttcttga
    68281 tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact
    68341 taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt
    68401 gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat
    68461 ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc
    68521 gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct
    68581 tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt
    68641 tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag
    68701 atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat
    68761 gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat
    68821 cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat
    68881 tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac
    68941 tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt
    69001 tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta
    69061 tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg
    69121 tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt acatattcat
    69181 tatacatatc tattcaaaaa acgcaacatc taacacttat aaaaagtaag tgttcatttt
    69241 gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt
    69301 aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca
    69361 actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg
    69421 atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt
    69481 cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct
    69541 tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca
    69601 aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca
    69661 ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca
    69721 tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct
    69781 tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat
    69841 tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt
    69901 cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt
    69961 ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt
    70021 ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt
    70081 agccagttgt tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa
    70141 tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct
    70201 cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg
    70261 cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt
    70321 ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt
    70381 tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat
    70441 ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg
    70501 cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt
    70561 gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa
    70621 gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta
    70681 cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt
    70741 gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt
    70801 ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca
    70861 gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct
    70921 gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg
    70981 accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgac gaattcttgg
    71041 acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct
    71101 cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg
    71161 atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc
    71221 agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact
    71281 ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac
    71341 ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct
    71401 caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc
    71461 agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt
    71521 cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca
    71581 gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg
    71641 ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg
    71701 tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta
    71761 acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat
    71821 gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca
    71881 gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca
    71941 tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt
    72001 ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta
    72061 ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca
    72121 tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg
    72181 tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct
    72241 gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc
    72301 gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt
    72361 ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc
    72421 atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc
    72481 tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga
    72541 tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc
    72601 tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg
    72661 gtttcttttt tctttttcat agcattcctg acagattcca tggaacgtta gccgatgatc
    72721 ttttactaaa aaatgataac gttcttctac tacttgctcc acttcaagta aaagatcctc
    72781 ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc
    72841 gccttctttt ctcaaatcat aacgggctaa tccatcatta aaacttactt tatcgacaat
    72901 tttcaattct gtcaagattt caagggttcg ataaacagta gcaagtccaa tttccggact
    72961 cttttttttc actaaaaaga agatttcttc tgcagataaa tgctctttct cattttccaa
    73021 aagcacaagc agagtagctt ctctttgcgg tgtcagctta aaccccgctt catgtagctg
    73081 ctgttttgtt tttttcatcg ccgaaatagc attcatgacc atttactcaa ccccaacaat
    73141 ttataataat tacaaataaa aaaagatata acactatgtt aattaaaatt attataagct
    73201 agttaagaaa aacatgcaag ggattctcaa ttagattttt tgactagttt tgtcacaccg
    73261 ttttcttgtg taatcaatcc ttgtttcaaa agattaccta atgcgcgctt aaatgctcct
    73321 ttgctgatac caaaagcttt catgatttct tctggatccg atttatctgt aaagttgatt
    73381 tgctgatctg cagaacgttc taaaatggtt aaaatcattt gtgcatcatc tgatatagct
    73441 tcatatgctc ttggcttcaa tgaaagattc agtacaccgt ctggtcggac accaatcact
    73501 cgacctttta tctgctcgcc tagacgaggt tcattatatc gttcagaagg atggataaac
    73561 ccaagataga aatcttccgt taaaacgtac gtacctgtta atttcaagcg gtatgctgtt
    73621 cctatcacat ctttgttttt catttcttct gttccatatt tgcttaaaga tttgaatatt
    73681 ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat caactctaag ggaaatcatc
    73741 aagcgatccc cttttttagg ccatagctct gtcattgtcg gcaattcatc aagagatacg
    73801 acaaagtctt tatctggaag accgatatcg acaaacgcac ctaaatcttt tcgtgtagca
    73861 gtaacgattc caaaagcgta atgaccgata cggcttttag gaatttgtgt agtgatccga
    73921 ttttcatgtt tttgattttg gtaaccgaat ccttcaactg cttcaccgat tttgtgttca
    73981 ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta gtttttttca
    74041 ttttcatcaa taataatacc tgtaaatata gttgctaata aatcgttcat ttttttctcc
    74101 ctctatagtg aaaggttttc ttaatacatt tctttgctat cataacataa tgttatctac
    74161 ttgaatagct gagccttggt ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca
    74221 tactcccctt tttcgaataa acggtgttaa acggcaggct cacagcctct ttcatttgcc
    74281 taaaattaat tgctcccatt tcctccgaac catccagttt tacagttgat ggttacgatg
    74341 taaaatggca aagcactttc tccaatgcct ttcatcaatg cagattttgt tgaagtggca
    74401 tatagattat aatagccttc cccattttcg attctggctt tttctaatct gtaacctcct
    74461 tcaacgatat atccacgttg gatacatgta gcaatcactt tgctttggac acctggtgcc
    74521 cacgtccatg ctggattaga tgcatcggca atatcacttg ctctggcaga tttgattggc
    74581 tgattcgctg agtttgaaga ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt
    74641 gcgaccgcag cttgttgagc ggcttgagct gcagctgctg cttgagcggc ttgttttgct
    74701 tgttcttcag ctgctttttt cgcttcttct gcttttttct tttcctcttc ttcttttttc
    74761 tctttatcag tgagcatctt atctacttgt ttcaattgtt cattcaactg agcagataga
    74821 tctgtatttt tcactttttc tactgcttct tttgccgtct tgtattggtc gcgtgttgct
    74881 gaatccacta cttttccgtc tttaaagact atagctgttt tttctttagc tgtttgcaat
    74941 tcagcatatt gatttttagc ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg
    75001 ttatccgttt cgattgcttg gatctcttta tctgctttca acttgatgtc atctgctaga
    75061 tgatcatcga cgatggcagg tgctatgaac cactcattca ctttttggat atctcggatc
    75121 ttagattgga tatcttggta agttgtttct aactctgaat atcctttttc attcttaaga
    75181 ctggataaat cattttttag ttttgataaa tcttgatgaa tcatatctgc acgaataaat
    75241 tgatggtgat catctgtata aaaggcagca agtttctcat gaagttcttc catttgttgc
    75301 tgtctttttg cttctgctgc tacttcggct tctacttttt gatgatggtc ataataagcc
    75361 cagcttcctc caccgagtag aagtactgct gctaaaacga gataaggtac tttttttgat
    75421 tttttctttt gcgctttatc tggatctgtt ttttctaagt ttgattcttg atgtgttttt
    75481 tcagtatctg gcacgattgt tattgatttc gcttctgctt caaacagctt aggctggttc
    75541 tcttcttttt ctatttttgc ttcaagatca gtaccctctt tgcttacaga ttgattttca
    75601 gcatcggatt cactatttat ttcatcgggc tctgttgact ttttgtctgt gttttcgagt
    75661 atttcttttt tctctgcttc cggcagttta ttcgtttttt ccttttctaa ttgtttttca
    75721 gtaaatactt cagttttttc tgtgatatcc gtttttacgt cttttatttt tactgtttct
    75781 ttacctgtgg cgttttctct atcagagtct ttttcagttt cttgttcctt ttctggctct
    75841 actttgtttt cctgtgcaat tttttcactg atcattttga gcagctcttt cgcgtgctct
    75901 tcttttgtat cttcacgatg tgctttgata taagcggata gtatcgaatt ttcttcctca
    75961 ttatcgacga tttctttcag ttcagctact cttctttttt gttttgattc aagcgtttct
    76021 tcttgattct ttcctgattc ttccgaagtc tcatgaacag ttgctttctt ctgttcattt
    76081 ttctctatat tagttacttg atcgtgagca ggtggttctt cgtcttctgg ttctgctttt
    76141 tcgaataatt ccattacaga ttctagaggt aaatctttca attccgacca ttcgattgga
    76201 tcgtttaatt ctgtatcagg aaagactgca tcttcagttg atttatcctg catagctgca
    76261 tctgtatttg tttcttcaat tgattctttt atttgctgat cttctgcagt tatttgatga
    76321 ccgcagtctg gacagatagt attctgctcc atttttttcg atccgcaatt tggacatttt
    76381 ttcatcactt agcgatcggc tcctttacta ggtttacgct accatttaat ctatcatatt
    76441 ctatacgtca cgccaatagc ttatttttag gatttactta aaaataatga atgaatttcc
    76501 ttcctataga aaaaaaagct tgagactgcg tagtctcaag ctttttttct tttaaaataa
    76561 accaaacagt tttactgatt tctcttctgg tcaatagtcc acgcttattc taagaaatct
    76621 tttaattgtt ttgaacgaga aggatggcgc aattttctta atgctttagc ctcgatctga
    76681 cgaatacgtt cccgagtaac accaaatact ttccctactt cttccaatgt tctcgtccgg
    76741 ccatcatcta aaccaaaacg taagcgaagg acattctctt cccgatcagt caatgtatct
    76801 agtacatctt ccaattgttc ttttagtaac tcataagctg cgtgttctgc tggacttgtt
    76861 gcttcctggt cttctatgaa atcacctaag tgagagtcat cttcttcacc gattggcgtt
    76921 tcaagggaaa caggttcttg cgcgattttc aagatttctc ggactttttc tgttggaaga
    76981 tccatttccg caccgatttc ttctggcgta ggttctcttc caaggtcttg cagcaattga
    77041 cgttggatac gtatcaattt attaattgtt tcaaccatat gaactggaat acggatcgta
    77101 cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa tccaccatgt agcataggtt
    77161 gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg ccttcatcag tcccatgttt
    77221 ccttcttgga tcaagtcaag gaactgcatg cctcgtccta cataacgttt agcgatactt
    77281 actactaaac gaaggtttgc ttctgccaga cgctgtttcg cttcttggtc gccttcttcg
    77341 attttcaatg ctaacgctac ttcttcttcc gcagttaaaa gggagacacg gccaatttct
    77401 ttcagataca tacgaaccgg gtcattgatt tttacaccgg ttggcgcaga aagatcttct
    77461 gtttttgctt gttcagcttt tttctcagca ctcttcaagc tgtgaattga tggatcacca
    77521 ttttcatcca ccacactgat acctgcgtct tcaatctttt ggatcaattt gtccatttca
    77581 tctgcattca aagtgaatgg tgtagcaagc tgattggata aatcatcata tacaacttgt
    77641 cgtttgggtt tgttttcttt gatgaaagct gctactgctg cttcatacgt ttttttatct
    77701 gttccatttg ccataaaaga aggcccccct tgagtagcta tttggcttgt tttaactgtt
    77761 tcgttaaatt gatgatttca atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc
    77821 tggcttcttg ctgcatttgt ttttttaatg ttatttcatc agctaagcca gatttgctga
    77881 tcacatctag caagtcttgg atttctcgtg gcgaactttc ttctgccacc ggtaaatatt
    77941 cgatttccac caccaaccgc cttaaatttt catcttgcaa aaagtctaaa aactctgcaa
    78001 ggttgaattc ataatgttgt gagacatatg catcgaataa aaaataaact tctgcatatg
    78061 catcatgaat aaactgaaat tctcgttctt ttaaaatatt acggacagaa acttctttaa
    78121 atgcacgata taatagaatc tgctctgcct tttctacttg cgtcaaaggt ctctttttcc
    78181 gtgcagtttc ttgtctcgta acctgagtgt gttgactttc ctgaggttct tgacgatttt
    78241 gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa
    78301 actctgttga cagttgagat agatacatat cttgttcgat tggtgaaaca actcggctaa
    78361 gttcaactag taattcttcg aggtattcga tcttgtcttt ttcattatct aaatttttcc
    78421 cttgttttaa ataacgtgat ttaaaagtaa agactgtttc gcgcccatga gccactaatt
    78481 cttggaaaga tgcttctcca tacttacgga gatattcatc aggatcaagc ttttctggaa
    78541 cgactacgac agaaagtgct aatcgactgt tttgccgaag caattcaatc cctcgattgg
    78601 ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac caattctttc gtcagctttt
    78661 ccatttgttg gatctgttga gctgttagac ttgtccccat agaagctaat ccagttttga
    78721 taccagcctg ccatgcagca atcacatcca taaacccttc gaacaacaaa atttctccgt
    78781 ttttccggat atcgcttctt gctttatcaa gattaaataa aacttgtctt ttattgaaca
    78841 gttctgtttc tggactattc aagtatttgg gctgatcttt ttcctgccct ttttcaggtg
    78901 caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga attacgaagt ggaaacatca
    78961 ctctttgata aaaacgatca gacatacggc catcatcatg cgtgacaaac aatccagagc
    79021 gttctaacaa ttcatttgaa attgattcat tcttgaatac ttgctgaaga aaagaacgct
    79081 cattaggtgc aaaacctatg ttgaattctt caattaattc taaagacaac ccacgacttt
    79141 gcaaatattc taaagctggc tgtccagctt ttgtatgcaa tagcatgtga tgatagattt
    79201 ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc agattcagga ttttggacag
    79261 ctatagtacg ctgacgccat tgatcttcta acggtatctg ttcgaagtct gccactttta
    79321 ccactgcttc tggaaaactt agaccttcta gttcttgaag aaaagtaaac acgtttccgc
    79381 ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg
    79441 tacgttcttc atgaaaagga cacagcccca agtaattttt accagacttt ttcaactgga
    79501 catattgtcc aatcacctca acgatattcg tttgactgcg tactgtttcg ataacctctt
    79561 gaggaatccg ttgagccatt ttttcacccc ctgctctatt gatatctttt gggtttaaca
    79621 gaagatattt caggagaata accggtaaaa aatcgcacta actgaaatca gtgcgatttt
    79681 aaaggacaca attatacatg tttgattgta tcatatttca ttgctttttc aagtataaca
    79741 catttctatc tcattaagca tcaatttgct actcttttca gtaactgatt ttgaatatca
    79801 gccttccagt acaatactcg aagaacaata tttacgagca tgaatacaaa gaagtatcct
    79861 acgctctctc ccaaccctaa gtttacttcc aaaattccat ggaacagcat tgcagctaca
    79921 tatatcccta cgatcgtcgt tactgctagg aagatgcgca atgggttgaa gggataacaa
    79981 gcttttacaa ctgccatgca actgatccca atcagcaagt aatacatcaa agtagtagtt
    80041 tcagcatttc cccatgcgaa tgtttttcca agtagataca ccgcaatgat attcactact
    80101 acaagcaagg catttggtaa agcattaata agtgctgtag gcaaataacg gtaagtgatt
    80161 tttcttttat cttgttcaaa agataaaaag aaggaaggat acccttcaat cgctaaatca
    80221 atcagcgtga tttggatagg gataaacgga aatccaattg ctgtaacggc acatataatc
    80281 gataaaataa atgaataaat cgttttgata aaaaagatgc ctgagacttt cgtcacattg
    80341 ttgaccactc ggcgtccttc aaacaggact tctggcaatg tagtaaaatc agagtccaac
    80401 aataccagat tcgcaatctg acgtgttgct ccatcacctt ctgccatagc aatgctgcaa
    80461 tctgcttctc ttaacgccaa aacatcattc acaccgtcgc ctgtcattgc aaccgttctg
    80521 ccgctttctt tcaattcatt taccagcaat ttcttttgct gcggactcac tcgtccaaaa
    80581 accgtatagc gatgagcagc ttctcgcact tccatttctt cagtaatttg agaaagatcg
    80641 acatacgcat cataattcgg taatcctgca cgtctagcga tattggagac agtaaaagga
    80701 ttgtctccag aaatcacttt cagatccaca ccttcctcat gaagataagc tagtgtttct
    80761 tgagcgtttt ttcggattgg atcatcgatt tcaaacagag caaccggctc aacaaatgct
    80821 ggtttttctt cgttcaattg ctgatttgga gcgatcccca acataagtac gcgatagcca
    80881 ttcgcttggg ctgtttttag actgtccgga agccgatctt cttccaccaa acgttcaggc
    80941 gctcctacgt acacgactcc cagttctgga aattccatcg cgccccattt gcgatcagaa
    81001 gaaaatgcga ctttgtttgt cacatcatac cgattagaaa gctcaaaata atctctcata
    81061 gcccgcatag tgatgttgtt atcactgctt tctgccagat acgtccccat aatttctggg
    81121 atggctttct cgtacagcgg atgtagtgct tctacccgct ggatactcat tttcccttca
    81181 gtaatggtcc ctgttttatc caagcataat gtatcgacat gggccagcgt ttcaattgaa
    81241 tacatgtctt gaacaaggat tcgttttttt gccagtttgg ttacccctgt cgttaatgca
    81301 atacttatta gcaataccaa accctttgga agcatgccta acaaagctgc tgcagatgca
    81361 ataacagaaa ttttgataga agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc
    81421 cccaaaggaa taatcacgaa actagtgaat ttggatactt tgcgaatcga atctacaagt
    81481 tcagaacgaa tagctttgtg agtctttgcc tcgctagtca agcggacagc ataattatct
    81541 gcacctactc ggataacttc agccatgaca gaaccgcttg tcaaaaagct tccagaaagc
    81601 aattcagctc cagtttcttt gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt
    81661 gcttctgccc gtccagatat aacctgtaaa tcggctggta tctgttctcc tgctccgatt
    81721 tttaccaaat ctcctaatac cagctcttta gtagaaatcg attgttcttt accgtcccgg
    81781 atcactagta cttctgattg ggaaacaatc gataatttcc gtacaagatt tcttgcatgt
    81841 atttcttgat aaattccaat aacgatattg acgacaataa ttgctaaata cgccatgttg
    81901 gaatacgcac ctacaaataa cagacaaatc ccaataccaa agttcagaaa attgaataac
    81961 gtcaatatat tatcgctgaa tatttgtttt gtactttttg atgcatcttc ctggtagtca
    82021 ttttgctggc cagaagccat tttctgaact acctcttgct gtgtaagtcc attcattttc
    82081 tttttctcca ttttccgcca actttcaaaa ataatcacgt gtttaagtct agcgtgttcc
    82141 aagattaaaa acaacttgtt tttataaaat ttatagaaaa acacacgaat gattttacaa
    82201 aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg tttttattat caatctatct
    82261 ttacttatat tgttcctcca aacggctcat ccaccaacct agtgctgtac cggctataaa
    82321 aagaacagga caagctagta ccccagcaac tcccaatccc gcgtaatcgg taggaatgat
    82381 cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg aatagcttgc tatatgcctt
    82441 tgagaaaatg taatccatga tttttgacaa tccaagaaca caaagaatcc caccaattcc
    82501 gatcggcaca atcacttcaa gtcgtacttc tttaaatccg tcagccatcg ctttatacaa
    82561 tcccatatac accaaaaaat tagaagggct caatcctggg acgatcatac ccaaaccaat
    82621 caatccgccg gcaatcagcc atgttccaaa attctgatcg acttgcccgt caaataattg
    82681 agcgccgtat aataaaaaca aataagccag aacaaaagta acaaccatga tgatcacatc
    82741 agaagtatct cttccttttt tccctgcttc tttccataac gcaggaacag ttccagcgat
    82801 acatccgaca aaaaaccata atatcgttgt ttcatacgca cctaataaaa aactgactaa
    82861 aaaagaaagt agaaagatgc ctgtcactcc gcctagacca acaggcagaa aaaacaaaac
    82921 attttctttg aaattttttg taaggtgggc taaaaaagaa atcagccgct catacatgcc
    82981 aaagacagct gctaatgcac caccgctgac tcctggcaag ataaatcctg aaccgataaa
    83041 cattcccttc acaaaccgta acatccagtc tttggttgtg taccccactt cttgattttc
    83101 cattttttgc cttttttcca ctatttgcgt actccttctt tcatttaatt ttattcatga
    83161 ttgaacattt cctgctattt tacttaaaga ctttttcata ggtctctatt attttttcat
    83221 tcattttgac aatatcagga taaaacatgt tacctccatt atgatagaga tagccgccat
    83281 tgtaaagcaa ggactggatc cgccaatatc ggtattgttt cccttcctca tttcctaaaa
    83341 gtgggctcag cacatcttta gaatacgctt ctgctaattc tactgtgttt tctccgccat
    83401 gattttttac ataggcaata taatctttcc caaaattata tgcttggaca gctgtccata
    83461 aatcgcatcc ttgtttcttt gcttctacta tcatttccgc caaatatttg acaccttgat
    83521 caatactgtc agctggatta tcaatgatct cagcttctcc atgcagactt tccgagctct
    83581 gcatgagatc tgtccctttt ccttttgatt cggtcaatat tatcgccttg atcaaattct
    83641 cgtagttttc catatgataa ttttttgcag ctgttgttat ttctatttga tagctttcca
    83701 ctcgttttac cgaacgataa gttcttcctc ataaaaacaa caccgcacca ataaccgtca
    83761 gaatgatcat gtaaaaaaga atacggataa tagataatcg ttttcgcttc atttcatcac
    83821 ctagttattt tgacatcttt ttttatctta ctatttcttt atcttcttgg ctatttcttc
    83881 cttgatttct gtgtacgcct taagaaaaaa tgaattttcc tgtatttttt gacatcttac
    83941 gaaaatgatc gttgtttttc agtaaaaaca attgcccaat gactgatata atcgttatac
    84001 tttacacagt aggtgattat gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga
    84061 gcgaaattta ctatgaaaag agcggtcaag gcttccctct ctttttactg catggcaatg
    84121 acggcagcgg ccgctttttt tcagaacagg tccctgtact cgaacgctac tataccgtgt
    84181 atctagtaga tagccgagga catggtcgtt cgacaaacga agcatctatg ttgaattttc
    84241 agttaatggc cgaggatctg aatacgatca tgttgctgga aaaaatcgat caggcagatt
    84301 ttcttggatt tagtgatgga gcaaatctag cactggtatt tgccagcagc ttccctaaaa
    84361 aagttcatcg attgatttta aattctggaa acacgcttgt taaaggcgtt cgtttttctg
    84421 ccagagtcat cagtaatatt cattatgcat gggtatggtt actctcttta tttcgcccaa
    84481 gtcttcgcaa aaatctttta gttatcaaac ttttgctgca tgacatcggg cttacagaaa
    84541 atgatttaaa aaaaatcaac tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca
    84601 agctaaaaca ttctttgtat atagctaaaa ctataccaaa agcttccttt gtgcttgtga
    84661 aagaacaagg acatgaatta gctagaaaag atcctgaacg cttcaatcga gaagttcttc
    84721 aatttctttc tgaaacataa ctattaggaa tagaaggtga tttatttgtt aaaaaatacc
    84781 atacaatggc tgaaaaacca cctaggactg ttcaaaacaa tctttttgat ttctgtcatt
    84841 gtcattattg tcggtgaatt gatgtccatc ggtaaaactc tatctatcca gcaattagga
    84901 gaaacgtttg ccactatacc tgtatggaaa agcggactaa tgctattgat tggtcttatc
    84961 tctgtgttac ctatggttgg ttatgatatt attttgaatc ggatgcttga acaaaagcag
    85021 aatcctcgtt acctgtttga gacaagctgg ctgatcaata cgatcaacaa tattgccggt
    85081 ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat atggacaaaa gaaagagagc
    85141 agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt tgatggctgg actttctatt
    85201 tacagtctgc tttctttttt acttgtagtc ttcacgccag ttgctccttt tttgaagcaa
    85261 tattggatat ggttgatcgg cggcggtcta tattttcctg tcgtcttttt atttactact
    85321 ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa ctcaaggaca gctattgcta
    85381 gtttcattgt tggaatggac cggtgtcctg tttagtttcc tttctgttgg ttatttaatg
    85441 gaaatccgga ttgatctgtg gcaaacaatt ccattattca ttgctgcttc agtgatagga
    85501 atcgtgtcta tgattccagg agaaattggg agtttcgatg taatgatgat tatcgggttg
    85561 tccgctattg gtgtgccaag agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc
    85621 tactacatca ttccgttttt gattggaatc gtctttttct tcaaaaatat cggttccact
    85681 tttgatcagc gttattctgg tatccctaaa caattagcaa cagaaattgc gcataaaatc
    85741 gtggttgtct tattatactt ttctggaatc atgctagtac tttctgccac tatcccgcaa
    85801 gcttttacag agtttcgctg gcttcatagt ttgaacccat tgaaatttca ttttattatc
    85861 caatttccta gtatactatt gggatttctt ttaatcatta tgggcagagg gatcgcagca
    85921 cgtgtgaaac gagcatattt gcctacaatc ttcttgattg ttctagcttt actttatgtt
    85981 ttattaagtg attttagttt cacaccggtg gttttcttgt ctattctgct tctgatcatt
    86041 cttgcttcta aaaacgaatt atttcgggaa caattgatct attcatggga gtggcgtacg
    86101 atcgatggaa tcattattgg tgcactgtcc ttgctttaca tcgttattgg ggtatacaat
    86161 ctgccggatt tccctcatcg gcgtcaccat tttatttcct ttttcttatt tccttctgaa
    86221 aagatttggt tttcgggatt gttggccatc atcgctgtta gtttcatgat cgttctattt
    86281 gttcatttcc ttcaaggaga aaagaaacag attggcgaag cctttaatga agaaaaggcc
    86341 ctgaagatcc ttacaactta tggaggaaac tccgatagtc aattgatctt cttaaaagat
    86401 aagcggatgt ttgcctatga aaaagacggt gaaccaactg ttcttctcca attcgcttgc
    86461 ttcaataata aatgtattgt aatgggtgat ccttctggta agaaagaaga ttttccagaa
    86521 gcaatcgaag cttttatcga agaaacagat cgactatgct atttaccagt tttctacgaa
    86581 acaagcgaag agattgttat gattttgcat gaattcggct acgatttcat taagatgggg
    86641 gaagaagctt atgtagacct gaatagtttt acaacttcag ggaaaaaaat gaagggcact
    86701 cgtgctgtct tgaatcggat cgaacgagaa ggatttacat ttgatgtctt acaaccacca
    86761 ttttctactg agcaaatgag tactttcaaa aatatctctg ataattggtt gggttcacga
    86821 aaggaaaaag ggttttcttt aggctttttc tctgaagatt atctacaaag agcgcctatt
    86881 gcagttgtca aaaataccca agaagaaatt gtcgcttttg caacgatcat gcctacttac
    86941 acgaacaata aagtaggaac gattgatctg atgcgatatg attctaaaaa agcaccttct
    87001 ggcagtatgg atttcttgtt cttgcatttg tttgcctata tgaaagaaga acatatccaa
    87061 tggttcaatt tagggatggc ccctctttca aatgtcggaa cttccagaaa gagtttttta
    87121 caggaaagga ttgcatactt ggtttacgag tttggttctc atttttattc tttccatgga
    87181 ttaagagaat ataaaagcaa gtacgctaca aactgggtct cacgttatac tctatattct
    87241 agagatagtt ggattgcata tgttatgatt gctttgctga ttattgataa tgcgccagtt
    87301 gagcagacac aaggtagaat aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa
    87361 ctgcataccg atagatgaac actctcatga tgaataaaag agactgaagt aaaacgaaag
    87421 ttcatgcttc tacaatctct tcttatacaa ttgaagaaca aagagcggtc caaacagaaa
    87481 tcctgtttgg accgctcttt tattttaaac atttttcatt accaaaatat tcgagtcgcg
    87541 tttcttatgc ttcgatctct gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa
    87601 ttgagaatgt cgttccgttt tcaacagcga ttggatggat caagtcaaca tcaatcgtca
    87661 cgttgtcacc aggcatgacc atttctgtat cttctggcaa tgtgattgtt cctgtaacat
    87721 cagttgtgcg gaagtagaac tgtggacgat agttgttgaa gaatggagta tgacgtccac
    87781 cttcttcttt tgtcaacacg tacacttctg ctttgaattt tgtatgtgga gtaatggaac
    87841 ctggtttagc aagtacttgt ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca
    87901 cgcctacgtt atccccagct tccccgtaat ctaacgtttt acggaacatt tctacacctg
    87961 ttacaactgc tttttgtgtt tcaggtttga tccctacgat ctctacctca tcaccgacac
    88021 gaacagcacc acgatcaata cgacctgagg caacagttcc tcgacctgta attgagaaga
    88081 cgtcttccac tggcaatagt aatggtttat ctgtatcacg ttctggtgtt gggatatatt
    88141 catctactgt atccatcaat tccataatag cagcttcagc atctggatcg ccttgcaatg
    88201 cttttaatgc ggaacctttg atcacaggag tgtcatcgcc tgggaaacca tattcgctca
    88261 ataactcgcg aacttccatt tctaccaaat cgatcaattc ttcatcatcg actaaatcaa
    88321 ctttgttcaa gaaaacaatc aggtatttta caccaacttg gcgtgacaat aaaatgtgtt
    88381 cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac taagattgca ccatccattt
    88441 gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg tcctggtgca tcaatatgcg
    88501 cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt attgatcgta atcccacgtt
    88561 cacgttcttc tggagctgca tcaatactag catagtcttg aggatttgct aggccttttt
    88621 tgcctaatac tgtagtgatc gcagcagtta aagtagtttt cccatggtca acgtgtccaa
    88681 tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg ttcttttgcc ataatttaca
    88741 aacctcctaa ataatataga tcactgaaaa atttcagctc tcaccttttt attatagtct
    88801 ttttttgact aaatcaaaaa gaatacgtcc gacttacttt ttgtaagatt cgaactaagc
    88861 ttattatacg tcaatattgg tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa
    88921 aaaagtacct tttataaata aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt
    88981 aactttcttt tgaagcaata agaacaaaat gaggaaaagt ttaaaattca gctgttgcca
    89041 acaactttca atagacgaaa tcttctattt cgttaataat aaggaaagag gtgaagtaca
    89101 tgaaaaaatt aattggcgta cttgtcgttt taggcatatt tctcggcgga agttttgcag
    89161 cttatcatta tttttacggc ggcgaagctt attatacaaa aatcacgacg aatggcgaaa
    89221 tgtcagctac ccaagcagat aacggagaga aattcacgac ttatacctac aagcaagcgg
    89281 cttataataa agatggtgaa aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat
    89341 taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg taaaggtgta ttaagctggg
    89401 aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa
    89461 tggtcttaaa attttattag ataaaaagag gctgggacag aagcgtttaa ctccaagaaa
    89521 taagaaggag ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg
    89581 ttctttactt ttgtctcacg ctcttttttt gttatattcg tcttttccga tccttctctt
    89641 gacacaattc atttaactga tcactggtac ttgaacacct aatattctag tcatttttat
    89701 aaacgattca atacttcatc cacatttttg atcatcacgg atatcgtacc atccggatta
    89761 ttcacaaatt caatcaaatc tgggttgcga tatacatcta ctggtacgat cagttcgatc
    89821 ccgtttgata gcttgaattt ttgttttcca aattttttct cagaaatttc ttgtacttct
    89881 tttacaggag gtgcttctgg gacaaatcct tgctcttgta cttcttcttt aaaagccatt
    89941 tttgccgtga tgttttcctt gaagactttt tccgcaatca tctcatgatc caaccgacct
    90001 ttttcttcta tcgtatcata gacagcttct ttcaaatcag ccatgatatc aaattcttct
    90061 gtctcaaatt tttttccaag atgcttggct actttttgga tcacttttac attttcttct
    90121 aaagaaggtg caggaacact ttcgatcact cttccagaaa aataaagctc ttttttgcca
    90181 gaaaactcat attttttttc aattaattcg taggtccaat cagtcaaatt cacagcgaat
    90241 gcttcatctg ctttttgtgt tttcgcccct agaattgctc gattcaaaat caagcggttg
    90301 tcgatccctg cttcgtctgc ttcaacaaag tgagtgtaag cctcacgata attgactttc
    90361 aataaagata agtacatcac tgtatctaat tcatacaaga caacaaatac atctgcacta
    90421 ggtgcttctt cactttcttg gtagatatcg aaccaccgct gaacgattgc ttcacttgct
    90481 tccacaaaat tttcttgagc gattttaagt gattcaacga tcatgctttc ttctgctagt
    90541 tgtccagttt ttgtttgagc agaagagatt ttttggatct tcttttgaag aaaatcacga
    90601 atatattctt ttgtcagatc cagctctttt tgtgaaaaga ctggagaacc agtttctctg
    90661 tcaataatat gcagaattgc tttttttaaa tagatatcca ttttctcatt cccttctgtc
    90721 ttagtttacg gaaaaaaaat caaaaatacc agaatcaaaa tcattttctt ttaaacaaaa
    90781 atttcaagag tagaaacaaa atggattctt tttttcgaaa aatgcgctac acttatagtt
    90841 gaacgaataa aaaaggagct gaaagtatga attcattaac aagcacttat cgtctatcaa
    90901 atggttacga aatcccggta gtaggattcg gcacatggca aacacctgat ggggatgtcg
    90961 ctgtttcctc tgttaaagaa gcgctggctg caggttatcg ccatatcgat actgcccaag
    91021 gatacaagaa tgaagaaagc gtcggccaag cgattaagga aagtggaatt cctagagaag
    91081 agatattctt gacaacaaaa ttatggaatg caaaccacag ctatgaactt gtgatgtctt
    91141 cttttgaaga atcattgaag aagctccaaa cagattattt agatctgttt ttgatccact
    91201 ggccaaaccc tgttgctttt cgtgataatt gggaacaagc aaatgcagat acatggcgag
    91261 catttgaaga actgtatgaa gcaggtaaaa tcaaagcaat cggtgtgagc aatttcttac
    91321 cccaccatct agatacacta gcaaaaacag caaaaattat gccgatggtc aatcaagttt
    91381 tccttgcacc aggcgaatta cagacaactg tagttgagta tgcaaaaaaa catgatatga
    91441 tcctcgaagc atatagtcca ttaggcactg gtaaaatctt cgatgtgcct gaaatgaaac
    91501 aaatcgctga agcacatgac aagacgattg cacaagttgc tttgcgttgg tcgctgcaac
    91561 acgaattcct tcctcttcct aagtcagtga caccaagcag aatcaaagaa aatactgaat
    91621 tatttgattt tgaattaaca gaagaagaaa tgaaacaaat cgatcagtta gatggtgtag
    91681 tcggcaaagc aaaagaccca gatactaccc aattttaagt cgaaaggtga ttttatggat
    91741 ccaatcgaac gcttgaattc tctctcggaa gaagttaccc agacttttca ttccgatttt
    91801 gtctttttga tcgatgcaga gaaaatccaa cattttcctg caagaaactg gacacatgat
    91861 caaatcatcg aagaactgaa aaaacggttc gatcattcgc taatggtcaa accctggcac
    91921 gaacatgaag tcatttattc acctgaacta cctgtttttg ccttgatacc gaaaaaatag
    91981 aaagaatgga acggattatg tatatataca taatccgttc cattttttat ttttctttca
    92041 aaaaacggga aaatacgcaa atttttctgt tgtaatttgc gtatacgcaa gtttttttgc
    92101 attactcctt ttcatttggc tgtttttttc ttataatcga aagggtatga gcgaaggaga
    92161 gaacatatga agaaaaaaca gaaacaaaaa ttattaaatc aattccgtcc ttcattggat
    92221 ggtgtaagag gacaattatt ccgttctcta gaagaagaga gcttgcgacg ttatggtttg
    92281 ccgttacaag tcatgcttga tcccaaaaac cgtcaaatct taacgatcgg attagctggg
    92341 aaaacaaatg aagatacacg gatcaacgtg ccaatcgatg acaacttcaa tactgtatta
    92401 aagagaattc gttcaggaga aaacgggatt ttcgaacgat tccgtgacaa tcttcttatt
    92461 gaaatcgtgt cttactggaa tgatcaacga ttgaaaaacg atgaaccaac tgcgcaagct
    92521 gttgcgacac cagttacaga agaaacagtc agtgtgccag aagaaaaaac ggaaaaacca
    92581 aaagcggaca caactgaaaa tacacgctca actgatacga atgccgcttt aagttttgct
    92641 gatttttcaa aagccgttgc tgagtatcct aaattttaca cagaacaaaa tgaacatgaa
    92701 gtagttatta atgaaaaaaa tgctgatgaa cctcgtaaat tagcaactat ttctatgact
    92761 actgaaaatg agttcgttat cgaaaaagca ctcgaacgga aatacaaagt caaattgact
    92821 ttgattcctt tgatcgaaca atttgcagcg acagcgatta ctgatcgata aaaattctat
    92881 ttgtcataaa aggctgggat ttaaaatccc agccttttta gtcatataat aatttctaca
    92941 gcaagtaaaa agaaattcct tctaacatca ggctcaaata tcagcttgac ctgtccaaca
    93001 aatcacctat gatcctacaa tgttaggcga cttctcttct acctgaagat atcttggagt
    93061 tgcctgattt cttaagatgc ctaaccttaa ctataaaaaa ttctaataat aaacatattt
    93121 tctgatagag aaaaaag
//
LOCUS       DANKMN010000002        84008 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000002 DANKMN010000000
VERSION     DANKMN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            38..427
                     /locus_tag="PW224_000090"
     CDS             38..427
                     /locus_tag="PW224_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDL2906813.1"
                     /translation="MKEKENYPVQHATKKRWTTVRYGNSDHYIHYDEILYLMSMPGYK
                     NKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSISRVNEIVTFAN
                     GEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            603..1346
                     /locus_tag="PW224_000091"
     CDS             603..1346
                     /locus_tag="PW224_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2906814.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1339..2919
                     /locus_tag="PW224_000092"
     CDS             1339..2919
                     /locus_tag="PW224_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL2906815.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(3170..3649)
                     /locus_tag="PW224_000093"
     CDS             complement(3170..3649)
                     /locus_tag="PW224_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2906816.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3665..4417)
                     /locus_tag="PW224_000094"
     CDS             complement(3665..4417)
                     /locus_tag="PW224_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2906817.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4534..4604
                     /locus_tag="PW224_000095"
     tRNA            4534..4604
                     /locus_tag="PW224_000095"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4566..4568,aa:Gly,seq:ccc)
     gene            4965..5690
                     /locus_tag="PW224_000096"
     CDS             4965..5690
                     /locus_tag="PW224_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2906818.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5945..6211
                     /locus_tag="PW224_000097"
     CDS             5945..6211
                     /locus_tag="PW224_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL2906819.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            6208..6639
                     /gene="ruvX"
                     /locus_tag="PW224_000098"
     CDS             6208..6639
                     /gene="ruvX"
                     /locus_tag="PW224_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL2906820.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6664..6969
                     /locus_tag="PW224_000099"
     CDS             6664..6969
                     /locus_tag="PW224_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL2906821.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(7050..8195)
                     /locus_tag="PW224_000100"
     CDS             complement(7050..8195)
                     /locus_tag="PW224_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2906822.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(8256..8903)
                     /locus_tag="PW224_000101"
     CDS             complement(8256..8903)
                     /locus_tag="PW224_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2906823.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            9097..9390
                     /locus_tag="PW224_000102"
     CDS             9097..9390
                     /locus_tag="PW224_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906824.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            9434..9745
                     /locus_tag="PW224_000103"
     CDS             9434..9745
                     /locus_tag="PW224_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906825.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            9742..10366
                     /locus_tag="PW224_000104"
                     /pseudo
     CDS             9742..10366
                     /locus_tag="PW224_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            10507..10665
                     /locus_tag="PW224_000105"
     CDS             10507..10665
                     /locus_tag="PW224_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906826.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            10666..11028
                     /locus_tag="PW224_000106"
     CDS             10666..11028
                     /locus_tag="PW224_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906827.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            11065..11913
                     /locus_tag="PW224_000107"
     CDS             11065..11913
                     /locus_tag="PW224_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL2906828.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            11903..13378
                     /locus_tag="PW224_000108"
     CDS             11903..13378
                     /locus_tag="PW224_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL2906829.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            13644..14051
                     /locus_tag="PW224_000109"
     CDS             13644..14051
                     /locus_tag="PW224_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL2906830.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            14054..14269
                     /locus_tag="PW224_000110"
     CDS             14054..14269
                     /locus_tag="PW224_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906831.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            14273..14653
                     /locus_tag="PW224_000111"
     CDS             14273..14653
                     /locus_tag="PW224_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2906832.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            14787..14942
                     /locus_tag="PW224_000112"
     CDS             14787..14942
                     /locus_tag="PW224_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL2906833.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            15011..15484
                     /locus_tag="PW224_000113"
     CDS             15011..15484
                     /locus_tag="PW224_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL2906834.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            15481..17175
                     /locus_tag="PW224_000114"
     CDS             15481..17175
                     /locus_tag="PW224_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2906835.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            17141..17326
                     /locus_tag="PW224_000115"
     CDS             17141..17326
                     /locus_tag="PW224_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906836.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            17330..18505
                     /locus_tag="PW224_000116"
     CDS             17330..18505
                     /locus_tag="PW224_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2906837.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            18498..20021
                     /locus_tag="PW224_000117"
     CDS             18498..20021
                     /locus_tag="PW224_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2906838.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            20077..20361
                     /locus_tag="PW224_000118"
     CDS             20077..20361
                     /locus_tag="PW224_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2906839.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            20348..20683
                     /locus_tag="PW224_000119"
     CDS             20348..20683
                     /locus_tag="PW224_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2906840.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            20978..21604
                     /locus_tag="PW224_000120"
     CDS             20978..21604
                     /locus_tag="PW224_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906841.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            22346..23182
                     /locus_tag="PW224_000121"
     CDS             22346..23182
                     /locus_tag="PW224_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2906842.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            23246..23677
                     /locus_tag="PW224_000122"
     CDS             23246..23677
                     /locus_tag="PW224_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2906843.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(23739..25061)
                     /locus_tag="PW224_000123"
     CDS             complement(23739..25061)
                     /locus_tag="PW224_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL2906844.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(25215..26855)
                     /locus_tag="PW224_000124"
     CDS             complement(25215..26855)
                     /locus_tag="PW224_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL2906845.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            27188..27658
                     /locus_tag="PW224_000125"
     CDS             27188..27658
                     /locus_tag="PW224_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL2906846.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            27880..28983
                     /locus_tag="PW224_000126"
     CDS             27880..28983
                     /locus_tag="PW224_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2906847.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            29267..29947
                     /locus_tag="PW224_000127"
     CDS             29267..29947
                     /locus_tag="PW224_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2906848.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            30037..31071
                     /locus_tag="PW224_000128"
     CDS             30037..31071
                     /locus_tag="PW224_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2906849.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            31085..31420
                     /locus_tag="PW224_000129"
     CDS             31085..31420
                     /locus_tag="PW224_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906850.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            31404..33884
                     /locus_tag="PW224_000130"
     CDS             31404..33884
                     /locus_tag="PW224_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906851.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            33940..35499
                     /locus_tag="PW224_000131"
     CDS             33940..35499
                     /locus_tag="PW224_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2906852.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            35496..36860
                     /locus_tag="PW224_000132"
     CDS             35496..36860
                     /locus_tag="PW224_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2906853.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            37031..38101
                     /locus_tag="PW224_000133"
     CDS             37031..38101
                     /locus_tag="PW224_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2906854.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            38116..38784
                     /locus_tag="PW224_000134"
     CDS             38116..38784
                     /locus_tag="PW224_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2906855.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            38804..39382
                     /locus_tag="PW224_000135"
     CDS             38804..39382
                     /locus_tag="PW224_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2906856.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            39783..40694
                     /locus_tag="PW224_000136"
     CDS             39783..40694
                     /locus_tag="PW224_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2906857.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(40782..41699)
                     /locus_tag="PW224_000137"
     CDS             complement(40782..41699)
                     /locus_tag="PW224_000137"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL2906858.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            41901..42338
                     /gene="zapA"
                     /locus_tag="PW224_000138"
     CDS             41901..42338
                     /gene="zapA"
                     /locus_tag="PW224_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL2906859.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            42397..42945
                     /locus_tag="PW224_000139"
     CDS             42397..42945
                     /locus_tag="PW224_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL2906860.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            43060..45420
                     /locus_tag="PW224_000140"
     CDS             43060..45420
                     /locus_tag="PW224_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL2906861.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            45507..45821
                     /gene="trxA"
                     /locus_tag="PW224_000141"
     CDS             45507..45821
                     /gene="trxA"
                     /locus_tag="PW224_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2906862.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            46003..47781
                     /gene="uvrC"
                     /locus_tag="PW224_000142"
     CDS             46003..47781
                     /gene="uvrC"
                     /locus_tag="PW224_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL2906863.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            47926..48825
                     /locus_tag="PW224_000143"
     CDS             47926..48825
                     /locus_tag="PW224_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2906864.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            48818..50080
                     /locus_tag="PW224_000144"
     CDS             48818..50080
                     /locus_tag="PW224_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2906865.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            50241..52139
                     /locus_tag="PW224_000145"
     CDS             50241..52139
                     /locus_tag="PW224_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL2906866.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(52469..53188)
                     /locus_tag="PW224_000146"
     CDS             complement(52469..53188)
                     /locus_tag="PW224_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL2906867.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            53525..53857
                     /locus_tag="PW224_000147"
     CDS             53525..53857
                     /locus_tag="PW224_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906868.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            53844..55847
                     /locus_tag="PW224_000148"
     CDS             53844..55847
                     /locus_tag="PW224_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL2906869.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            55866..56336
                     /locus_tag="PW224_000149"
     CDS             55866..56336
                     /locus_tag="PW224_000149"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL2906870.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            56357..56941
                     /locus_tag="PW224_000150"
     CDS             56357..56941
                     /locus_tag="PW224_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL2906871.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            56956..57942
                     /locus_tag="PW224_000151"
     CDS             56956..57942
                     /locus_tag="PW224_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL2906872.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            57932..58243
                     /locus_tag="PW224_000152"
     CDS             57932..58243
                     /locus_tag="PW224_000152"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL2906873.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            58265..60046
                     /locus_tag="PW224_000153"
     CDS             58265..60046
                     /locus_tag="PW224_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL2906874.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            60039..61415
                     /locus_tag="PW224_000154"
     CDS             60039..61415
                     /locus_tag="PW224_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL2906875.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            61422..62057
                     /locus_tag="PW224_000155"
     CDS             61422..62057
                     /locus_tag="PW224_000155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL2906876.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            62087..63442
                     /locus_tag="PW224_000156"
     CDS             62087..63442
                     /locus_tag="PW224_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL2906877.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(63634..65028)
                     /locus_tag="PW224_000157"
     CDS             complement(63634..65028)
                     /locus_tag="PW224_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2906878.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            65120..66091
                     /locus_tag="PW224_000158"
     CDS             65120..66091
                     /locus_tag="PW224_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2906879.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            66088..66870
                     /locus_tag="PW224_000159"
     CDS             66088..66870
                     /locus_tag="PW224_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL2906880.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(67039..67410)
                     /locus_tag="PW224_000160"
     CDS             complement(67039..67410)
                     /locus_tag="PW224_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2906881.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            67544..67987
                     /locus_tag="PW224_000161"
     CDS             67544..67987
                     /locus_tag="PW224_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL2906882.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(68030..68839)
                     /gene="yidA"
                     /locus_tag="PW224_000162"
     CDS             complement(68030..68839)
                     /gene="yidA"
                     /locus_tag="PW224_000162"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL2906883.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(68858..70225)
                     /locus_tag="PW224_000163"
     CDS             complement(68858..70225)
                     /locus_tag="PW224_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2906884.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(70209..71039)
                     /locus_tag="PW224_000164"
     CDS             complement(70209..71039)
                     /locus_tag="PW224_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL2906885.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            71202..71639
                     /locus_tag="PW224_000165"
     CDS             71202..71639
                     /locus_tag="PW224_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL2906886.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            71715..72335
                     /locus_tag="PW224_000166"
     CDS             71715..72335
                     /locus_tag="PW224_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL2906887.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            72524..73030
                     /locus_tag="PW224_000167"
     CDS             72524..73030
                     /locus_tag="PW224_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL2906888.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            73047..73946
                     /locus_tag="PW224_000168"
     CDS             73047..73946
                     /locus_tag="PW224_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL2906889.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            74033..74917
                     /locus_tag="PW224_000169"
     CDS             74033..74917
                     /locus_tag="PW224_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2906890.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(75109..75180)
                     /locus_tag="PW224_000170"
     tRNA            complement(75109..75180)
                     /locus_tag="PW224_000170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75145..75147),aa:Arg,seq:ccg)
     gene            75240..76574
                     /gene="rpoN"
                     /locus_tag="PW224_000171"
     CDS             75240..76574
                     /gene="rpoN"
                     /locus_tag="PW224_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL2906891.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            76723..77814
                     /locus_tag="PW224_000172"
     CDS             76723..77814
                     /locus_tag="PW224_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL2906892.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            77855..78856
                     /gene="gap"
                     /locus_tag="PW224_000173"
     CDS             77855..78856
                     /gene="gap"
                     /locus_tag="PW224_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2906893.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            78966..80156
                     /locus_tag="PW224_000174"
     CDS             78966..80156
                     /locus_tag="PW224_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL2906894.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            80260..81015
                     /locus_tag="PW224_000175"
     CDS             80260..81015
                     /locus_tag="PW224_000175"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL2906895.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            81131..82429
                     /gene="eno"
                     /locus_tag="PW224_000176"
     CDS             81131..82429
                     /gene="eno"
                     /locus_tag="PW224_000176"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL2906896.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            82828..83967
                     /locus_tag="PW224_000177"
     CDS             82828..83967
                     /locus_tag="PW224_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL2906897.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        28019 a        14439 c        17522 g        24028 t
ORIGIN      
        1 ttatgaaaaa aataaaaaaa gcatccaaag ttacttattg aaagaaaaag aaaactatcc
       61 tgttcaacat gctacgaaaa aaaggtggac aactgtccgt tatggtaata gcgatcacta
      121 tatccattac gatgaaatcc tttatctaat gagcatgcca ggttataaaa ataaaatagg
      181 cctagtattg aaagatgaag agattctcgt aaacggcaca attaaaaaat ataaaaaatc
      241 actcgaatat gagtatttat gtaaagattt aaaagcttat atcattaata ctgaaaacat
      301 tacatctatt agcagagtaa acgaaatagt aacatttgca aatggagaac aactagagat
      361 gggaaagaga agtattgata aactaaagaa ttttatgcaa gaaaagatag aaaatgaatc
      421 attttaaaga aaacaaagac tcatatctta cctgttagaa gagtgttttc ctgtagaaga
      481 gaacaaccca agtagcagcc tagtcgtttc tatccttttt tgttatgcac tctttttgac
      541 atttactcca ccaataacga agggtacaat aaaaagtaat aacaaacaaa aggagtacag
      601 aaatgattga attcatcaat gtaagtaaaa gttacgggaa aaaacaagca ttaaaagact
      661 tgtctctctc gataagacaa ggggaaattt ttggattttt aggacataat ggtgccggaa
      721 aatcaactac gattaaaagt ttagttagta ttattgaacc atcatcggga acgattttag
      781 tagatggaat gaaattgacg gaaaatcgat tgagtatcaa acaaaaaatc ggttatgtgc
      841 ctgattcccc agatattttt ttacaattga cggctggaga atattgggac ttgatttcag
      901 ctgcctatga attagacaca caaaaaaaag aaaagcgttt agcagaattg acagcgcttt
      961 ttgatatgta tagtcatcaa aatgaaacaa tcgcaagttt ttcacatgga atgcgtcaaa
     1021 aaacgatttt gatcggcacg ttgctgccag atccagatat ttgggtattg gatgaacctt
     1081 tgcaaggact tgatcctcaa gcagcatttg atttgaaaga aatgatgaag gctcatgccg
     1141 caaaaggaaa aacagtcatt ttttctacac atgtattgga taccgcacaa cagttatgcg
     1201 ataaactggc tattttgaag aaaggtgaat tgatttatca gggggcagtt agtgaattat
     1261 tgaatggctc acctgatgaa acgctagaaa agatttattt gaaaatggct ggaaggcaag
     1321 cgacaggaga tggaccgtat gtataagcga caattattag aattaattcg agttaatctt
     1381 ttatatgtta atccacagct cacgaataaa gcaagagaga aaggaaaaaa gggaaagcga
     1441 ttaactatct atcttttgtc tcaaaatctt ttttcagggc tgattttttt actgatttac
     1501 ggatttgcta tgtttgctat gaactttgtt aaattgccag gtttctttac ttattacgtg
     1561 gcactttttg gtatgttagg tttaactcaa agtatatcag tcatttataa tatctttttt
     1621 gaaggaaatg acctgcaagt atttttgcct ctgccttttg gacaagaaca ggttttttca
     1681 gcaaagattt tggtagttgc tttgaccatc attccttttg tttttccgct tctagtcatg
     1741 tttattttaa ccggatggcg agctgatcta gggattttgc ttactatcat tatctcaatc
     1801 cttttgtttt ctctattttt gattattctt ttttgtctgg ctagtttgat tgtttttggt
     1861 ttggctcgaa cagcagtgtt taaaaaacat agaaaaacag tttctagtat cttgcttggc
     1921 agctcgatgc tgatcgcatt tgctggaatc atgtggatga atcatcaaac ggggactatt
     1981 gaaaatgagt taacagatcg gcatccgatt tcgatattat tgccattcta tgatgctgcc
     2041 agtcagccac tttctcaaca aggtttattg ggttggggaa tactagctat cacggctctg
     2101 ggatttttcc ttgctgtaaa acagctgata gtaccaaagc ttacagagca actgactgat
     2161 tcaaacagtg agttcaaaat aaaaagaacc cataaaaaga accaaaatgt gcatcagctt
     2221 ttatttagtt acaatgctca attagtaaaa gaacctaatt tgattatgca agttctttct
     2281 tcttccttgt taactccaat catttttatt cttgcttttg ccctaggagg cgaaataaaa
     2341 ttaactgatt tagataataa gttaatcggt gttgttttcc tggtagggat cgcattggct
     2401 ttcttaacag ttaatcagac ttcttttatc agcaatctta tttctttgga tcaagaaaat
     2461 tttctgttta tccgttcttt gccaatctct atgaaccaat atttaaaaga aaagttcaga
     2521 ttcggatgga tattgcagag ttttttaact ggtgggatcg cattattaag cggactgtct
     2581 tttcagctgc ctttgttttt tatccctagc ttgctatttg gttctttatt cggctgctac
     2641 ttattgtcac tacgttattt cgctagagat tatcggttgc tgctgctgaa ctggaccaat
     2701 attaaccaat tatttaacag aggttcagga agtttgggtt tagtcgcaac tatgatgggt
     2761 tcattgatca tcagtattat tcttatcgtc ttatacggaa tgttggcttt gttttatcca
     2821 ttttggttag tgaatattcc tgtgatcggt ttagttctaa tactttccgc tctatggatc
     2881 gtttattatc aacgaacgtt ttggaaaaaa tttgaataaa gaagaggggc cgtgacaaaa
     2941 atcatgtcgc agcccctttt ttacagatag agggtgttca aaagtcagtt tctcagtatt
     3001 aagtcaattt tatgtaaaaa tgtgagaagc tatttttaca aaactttttt taatactcat
     3061 aattacttct tattgctcct gcggttactt gtcgcaatgt ttgtgagata ttttgtcatc
     3121 gctcaaggct gaacgtctct ttcacaacct tttctttgtt acacttcgtt tatttaaaaa
     3181 gccgtttttt tcgtttcatc atgattgcgt cacgctttac tttgaaatta cggatgttat
     3241 ggttaaagac ttctgataat acaagaccta aagcgattgc tccagcaatc ataattgctt
     3301 ggaccgcata atgagccgct tctcgatagg attcagtaac gagatttcga accgcttgat
     3361 aagctaaacc accagggact agaggaacca tcccgggaat attgaaaatc gttacgggca
     3421 ttttcaaacg tttagagaaa agatcgctga tgaatgctac tcctaaagaa ccaagcaatg
     3481 aaccaaaagc agcagatccg cctatttgaa cacagatcca gtagatcatc cagccaaaag
     3541 caccagaaag accacaacaa atcaatgaac gcctaggtac atttgtaata acggcaaatg
     3601 ccgcagtcgc taaaaaacta aaagaaaact gaataaatat cgtccccata cttgtgttcc
     3661 tttcctaata aaaaagctgg aaaataaaag caatcgcaaa accgatcatc gtagcagtca
     3721 tcatcgcttc tgttccgcga gaaactcctg agacataatg tccagctagc aaatcacgga
     3781 tagcgttcgt aatttgaact ccaggaacta aaggcatcac acatccgata atgattagat
     3841 cctgattgct tcctaagcca aaacgtacgc tgtagatagc ggctatgcca atcaataaag
     3901 aagataaaaa ttccgataaa aatttgattc taaaaaattt caaactatat agataagctg
     3961 cgtatcccaa tcctccaatg actaaagtca caggtaaatc ggtcatcttt ccgccaaata
     4021 acaccataat tgttccactg atcactgccg cgctaacgat ttgcaaccag acaggaaaga
     4081 aagaacggtc tcggtcgatt gcttgcagtt cgcagtaaag ttcttctaat gttagctctg
     4141 aagcaacata tttacgtgaa agatcattta cttttactac tttttctaaa ttaatcgaac
     4201 ggttggtaat ttgttccatt cggatggtcg aggtacgatc aagaccaata aagagaccgg
     4261 tttgagtgac atagctgacc aaacggaaat ttccagaagc taaagcaatt cgactcatcg
     4321 tatcttctac ccgatacatc tccgcattgc tttccatcat gattttccca gcgagtaaac
     4381 aagtgtccag caacagatct aattcttttc ccgtcatagc atcttctcca ataattgatt
     4441 gattagtttt attttactac aaaacatacc agaagcgtaa agaataaaaa aacttgagga
     4501 aggaataaaa gaggcgtata ctgtttttgt attgcgggtg tagtttagtg gtaaaatccc
     4561 agcttcccaa gctgatgtcg cgggttcgat tcccgtcacc cgcttaaaat aggttgtgaa
     4621 aaagctgctc tgcaacaagt aataagaaca gccaagcaaa aacaacttct tatgttttta
     4681 acttggatgg acttattacc gcagatgcaa gcttttgcgc accgtttacc aagcggttgt
     4741 gaaatcagtg tattgtcttg agtatgaaga aaatttttga ataataataa ataccttaca
     4801 gatagagtgg tgactgatta gagacgagga atccgtctct tttttgtatt tttaagccat
     4861 cttaaaacag gttttatttt ttgctgtttc tatttctttc tagtatttat ttatagggga
     4921 acatatgtta agatttaggg agctgttagt aaaggagtaa agaaatgagc aaaaaaacaa
     4981 ccaaggcgat cgtggggatc gtcgctgctg tgattgcagg ggcattcggt gtttccacca
     5041 cacaaaaaga ttcagatatc tttcagcaga ttacaactat ttttgattcc acaccacaag
     5101 cttctcaagt agcggatgtg ccagaatatg atggaaaaca tcaagaaata gaaatcaatg
     5161 ggaatcagcc ggaatttaca aaagaggatc ttagcctttc caaaggaaca tgggaaagct
     5221 attcaaatat tgataaattg aacagagtag gacaagccaa tgctatgctt ggaaaagatt
     5281 tgttccctac tgaaaagaga gagcctttgt atattgaccc gacaggttgg aaacaaaaga
     5341 aattaagtga cgggcaatgg ttatataatc gcagccattt aattggtttc cagctgactg
     5401 gtcaaaataa caacattaaa aacttgatga cagggacacg ttcgttgaat gctccataca
     5461 tgcttgctca cgaaaatgat attgcggcat atatcaaaga aacaaatcat catgtacgct
     5521 accgtgtcac tcctcatttt gaggggaatg aactggtagc caggggagtg caattggaag
     5581 cagaaagcat tgaagacaaa aaaattgaat tcaatgtctt tatctacaat gttcaagatg
     5641 gctacacgat caattatgag acaggacaag caacaaaaac aaaaaattag tagcagctat
     5701 tcgaattttt tatgaatatt atgaaaaaat tgtgtaaaaa catacattca aagaaatata
     5761 gcgtatactt tatgaaaaga gtacttttga taaagctaca gaaaaaagga aaaaaacata
     5821 caaatagtgt ttcttaattt attcggaagt ttggaatatt tgtttgtagt tttccctttc
     5881 ttcgagtaaa ataaggttgt agaaggtaag ctggaattac cttgcgggaa agaggtgtag
     5941 aatcatgggt tttacagatg aaaccgtacg ttttggtttt gacgatagtc gcaaaaaaga
     6001 agtgagcgaa acattagcga ttgtttatcg ggctttggaa gaaaaaggct acaatccaat
     6061 caatcagatt gtcggctact tgctttctgg agacccgtcc tacattcctc gttatcgtga
     6121 tgctcgaaat ttgattcgcc gtcacgaacg cgatgagatc atggaagtaa ttgtcaagga
     6181 ctacctctca aatcatggag tgaatttatg agagtaatgg gcttggatgt cggttctaag
     6241 acagtaggtg ttgctatcag tgaccccttt ggctggacag ctcaaggagt agagatcatt
     6301 cggatcaatg aagcagaaga agagtttggc tttgatcggt tgaaggaatt agtagcacaa
     6361 tatgaagtag accggtttgt agttggatta cctaaaaata tgaacaattc catcggacca
     6421 agggcagaag cttccatgtc atacggagaa aagatcaaag aactattctc cttgccagtt
     6481 gattatcaag atgaacgatt aaccactgtt caggcagaac gtatgctagt agagcaggca
     6541 aatacttcaa gagccaaacg aaaaaaagtc atcgataaat tagcagctgt gatgatctta
     6601 caaaattatt tagacggctc aggtaagcaa ttattttaat aataaaagaa aagaggtaat
     6661 cttatgacag aacataacca ttcccacgac catgaaggac acgaacacat cacattggtg
     6721 gatgaccaag gaaatgaaac attatatgaa atcctactaa cagttgacgg acaagaagaa
     6781 tttggacgta actatgttct tttatatcct gctggtgttt cagaagatga agatgtagaa
     6841 ttacaagctt atgcatacat cgaaaacgaa gatggaacag aaggcgagtt agaacaaatc
     6901 gaaacagacg cagaatggga catgattgaa gaagtattca atacattcat ggcggaagaa
     6961 gaagaataga ctataaagct aggagaaacc ttgatatgat agggtttctc ctttttttat
     7021 tttttactgt atgccatatt gtatgccatc tagtcaatat ttaagtgttg tactaatttt
     7081 tcaacagtct tttctttcgt ttcttttgcc aagtgaccgt aacaatcgat cgtctgacta
     7141 atttttgtat gccgtaatct agtttgaact tctaccatag tagctccagc gtccaagagc
     7201 attgaagcat gagtgtgtct tgctacatgc acaccgagaa atggaaccat agctttttca
     7261 caaatagtaa tcaatcttgc ataaatagcg tggtgtgtga ttagttcttt atagatacca
     7321 caaaaaatca tagtcggatc agttacacca agtgtcagca tatatttccg ctgattaatc
     7381 tgccattttt ttaatataga aattgttctt tcatctagtg gaagaacccc agcactacaa
     7441 tcagttttag gatcactgat tttatagcca tttttggatt ggcttagtgt tttattgatg
     7501 gtgaccgtat tttcttgaaa atcaatgtct gaccaattca atgcaagggc ttcactgatc
     7561 ctaaccccgc tataaaagag aaagcgtagt aacgttttat caaagtcgtt tcgataagtt
     7621 tcaggtttgc tgtttaggta atcaaacaag gtgatcagtt gttctttact gtaaactttg
     7681 accttttctt tatgcttagt ggtggttctt ttaggcatta atacgttgtc tagtggatta
     7741 gtctcaataa tcccaagatt gatagcgtag ctaaaaatac gcttagaagt tgaatgaagt
     7801 accttgtatt ggtcagtagt ttcagcccag ctatttacaa tttgttgggc tttttttacc
     7861 gtgacttcat tcacacgaag atttccatat tttggaagaa tccactttct aaacttgatt
     7921 tcagttttca tgaaggtggc ttctcggaca gtggttttat atgtttctag ccacaagtca
     7981 taaacttctt taaacgtttc gttgttcgct tttttaatac cgtttttatc gtagtccact
     8041 tgcaagcgtg acaaggcaag ctgggcttct ttttttgttt tgtaacctcg tctacgaacc
     8101 ttatcaggtt tgccagtcat tttgttcatt ccaagatagg cttcatagag ccaaaattta
     8161 ccttttttag tttcatattg cttgaatgtt gccataatag ttttccctcc atagtgtagc
     8221 aagccgtggg ggcggttgga ggaggcagtt tattattatt ttgaatagtg tttatttagc
     8281 tcagtaacaa gagctgtgaa ctcttttaga agatcatttt cataaaattc ttgatactct
     8341 tttttctcat ctgtattttt ttctgaatca ttaattaatg agtcatatcg taaaagcgta
     8401 tttagaagag ctgcgagata agcaattggg gtactcccgt caggaaattc aattcgagat
     8461 cctgttttaa gatattttat tgcgtttgtt aaataaatct tctgtgagac agtcaggtta
     8521 tcttcttcga tgttattgat agcattagaa agattagaat caattataga gttatttttt
     8581 tcaatagttt ttttcaattg cttttcaagc acatttcgct taatttcggg catcatatgc
     8641 atatctctta atgtataaga tccgtttaat agataggtag tggatacatc taataccttt
     8701 gaaagttgtt taagcctggt gttattggga agattatagc cattttccca attactaacg
     8761 gcacctttac tggccggtgg ggataattta cctccaagtt gttccattga gagtcccttt
     8821 tcaattctag ccagtctaat tctttgtcct gtctccactg cttcggggta tttttttcta
     8881 gccacatttt caccaccatt catatatttt ctctactata tcacgattta aatatgtgtg
     8941 aaacataaaa gttcaaaaaa gtatgttaaa attcttgcaa atgaaaaggt atgatggtat
     9001 attgagttta taagaggtat agaaatgtat tcaagagcct cttgcatttt tttaaaatct
     9061 ttgtatagaa atgtatgcta acgagggggg ataaatatgg aattaaaaat tgacaaactc
     9121 acgcttgatg atccagcttt taaaattatt gacgatcgag ttgttcaaca agtagagcaa
     9181 actcaaggaa cgttactttc attattagaa aaaaagtacc agtttccaaa atatatgaac
     9241 aaggcacaag ctgctaagta tatgaacgtt tcatacaata ctatgatgca gaaatatgtt
     9301 cctaacggtt taaagttgat tatagttgat ggagtcgtaa gaatttcaca agatgaatgt
     9361 gatcgcttta tggaagaata ccaaaaataa acgcaagccg tgggggcaaa gggattaata
     9421 ggaggaaata ataatggaat cgacacaaaa taaagcaatc gaaaaagtat tatcaacaat
     9481 tcttacagaa gacacagcgg cagaattagc aaacttggaa ggcaaagcgc tagaagaaac
     9541 gtttgaatgt ttgtacgaac aaatggacta tcaaaaattg ctaccacaag aaccaacggt
     9601 aagcggagta ttgcgtggct tgaacgattt agtacaagca gagtttaaag aacgactttc
     9661 tattgaagag tatcaagaaa ttttgtatca gcaagttgag ttgttagcga gcttgctagg
     9721 gcttgaactg gaggacgttg aatgaaaact gaaatttgga acggacacat tataagattt
     9781 gtcgatatta atgatgaatg gtgggcagtg gcgaaagatg ttgcggaagc gttaggtctt
     9841 aaacaggtta ctagagccat tcactctttg cctaaagatg gggttactac tagtaaggtc
     9901 atcgattcat tgggcagaac acaagatgta aacatcatca acgaaaaaaa atatttaccg
     9961 catggcattc aaaagccgta aaaaagaagc cgaggcattt caagactgga ttttcgacat
    10021 catcaaagaa ctacgccagt ccacaggact tgaaggcttt caagtattcc gcatgctaga
    10081 caaagaacac caaaaagaag caatgactaa attaagccac gctattactg aaccaaaacc
    10141 agtagactac attaaagcga atgtgatagc caataaagct gtatcaacta tttatggtca
    10201 ttccaaaatg gtaaaaaaga aagatatgac tcctgaaatg ttggttgatc gtgaacctat
    10261 tttagatgag acagtagaat taatgactgt taaagaaaaa tatgggttgc agttttcagt
    10321 gagtgagaaa atttataatc gttctgctga attgcagaca acttaggagc attaacatga
    10381 tcgagaacaa agaaaaagcc ttatccataa cagtcttggc ggacaggata aaggcaactt
    10441 aattgggata aaacccgata ttctttgttc cgattatacc atattaaaaa atcggaggga
    10501 accattatga aacgaaactt aattatagga agcattttaa tcggcggaat tttcggctta
    10561 ttaccattaa ttgcaaaaat agctatcttt gcaggattag cgttatatct cttcactgct
    10621 tatgacgagt acgactatca attaagaact ggagggcata agtagatgta caaaacaaaa
    10681 ctattaaacc aactcgatag ccttgaatta gaggaaatta atcaagggat cgctgaacta
    10741 gaaaataata tcgggaaaac ctactttgga aattcattca atgaaaaact aacagttttg
    10801 tatgtactca aaaaacatgc agaacacaaa ataatttgtc gagaaatcaa cgagctaaaa
    10861 aatcagattt taaccgcatg gttaaacatt actgatatgc aagaagcaag ggtgaaaaca
    10921 tttaatacat gggtaaagta tcagaatcaa ttaaaaggtg ctgaatttgt tcgggatggg
    10981 ttgaaatacg aacttgagca gttgaagctc atggaggtgt ctgaatgatt tataccagta
    11041 ttttgaaaaa ggaggagagg tttattggca aataattatc aagcagcaac ggaattacaa
    11101 aagtcgggat ggtcaattta tcccttatct cctggaacta aaattccaat aaaaggctct
    11161 agtggttgga atgatgcaac taacgattct aatcaaatta atgaatggtg gacggaagat
    11221 gcaactagaa atattgggtt aatgcttgaa gatcatcaaa tggtggtagt tgacctagat
    11281 caacattctg aaacacgaaa cggcgttgag aattataaaa aacttgcttc aatgtatgat
    11341 ccatttcctt ctacttatac ggaaatcaca ccaggtaaag gaatccattt cttctttaaa
    11401 attcctgatg gtgtaattat ccaacaagaa acaggtgcat ttgctgatat cttcgggcgt
    11461 gatgataaag gtaaaagttt aagcgggatt gatattatca ctaaaggagt tccaatagcg
    11521 ccaactatga ttatttcaag aagtgttggg agagcatatg aacctattga aaacgtatct
    11581 ctaaataata ttgcaccagc tcccgattgg ctagtgaacc tactgctaaa aaaaccgcaa
    11641 gaaataaaga ctcaataccg acccgtagca aaaacaggga cagcaaaaaa attagatatg
    11701 attgttcaag gcgctggcga aggtagtaga aacgatcatt taactaagtt atgcggttgg
    11761 ttactatggc acggtgtaga taatcaaact ttagcagaat tgatttatac agcaaatgcg
    11821 tacaatgaac caccgctaaa agataaagaa gtgaatcaaa ttattcgatc gatgattaaa
    11881 aaagatttga gggggaaagc tcatggctca taagcataaa ttagatagta ttttagactt
    11941 tcccgaagcc agcgagcgtg aagacaatat cattgaattg aaaacctgga tgtcacgatt
    12001 aagatgcaat aaagacgatc agattaaaag taacagtgtt gtgaatgcag aattgatttt
    12061 aaccaatgat agcaatttag ctggaacaat tgcttataac gaatttagcg gatacattca
    12121 cctattaaaa gattctcctt ggatcaatcg taccgctgga gaatgggaag acagttttga
    12181 ggatgcctta acggcatata ttgaagaaaa ctacaatgta gtttttgatg acaacaaaat
    12241 acacaaagca gtagtaaatg tggctagaaa gaatgttttc aacccagtaa aagaacgtat
    12301 cgaaaaagtt aaatgggatc aacagcctag actggaaaca ctgtttattg accttttagg
    12361 ggtagacgac aacctttaca cacgagaagt aacaaaacgt tggatagtcg gatcagtcgc
    12421 acgtatctat aaaccaggaa taaagtttga gatcgttcca gttttggatg gaccgcaagg
    12481 gattggtaag agtaccgttc cagcattact ttacacggat gattttttca ccgattcatt
    12541 agattcgcta ggtgaaaaga aagatgatta tatgcagctt caaggcaatg taattattga
    12601 acttggagaa ttatcttcaa tgaacaaaac taaaattgaa cagataaaaa acttcatcag
    12661 tgccaagatc gataaaatta gacctcctta tggtcggaat gtcatagctt gggcaaggca
    12721 atgtgtattt attggaacgt cgaatgatgg gcagtatttg aaagatgata caggaaaccg
    12781 ccgtttttat cctttgcctt gtaagaataa gccaaagatg aatccgttta aaacaaagga
    12841 tgaatatttt cttcaagtgt tagctgaagc aaaggtgctt tttgataaag gacaacgaat
    12901 ctattttagt cccgatgaag ataaagaagt cttagaaatt gcaaaagact atcaagagga
    12961 tgccaagatg gagaatccaa ttaaagaagc aatcactaaa tatttggaaa tggaaatccc
    13021 gtataaatgg gaagaagctc cagcgtgggc gagacgttct tattatcaga actatcctga
    13081 cagtaaatgt aatgagaaaa atttacagca tttctataat ggagctacta tgcaacaaga
    13141 gttttattta gttgatagcg tactcaccgc tgatattttg gaagctgttt ttgataaaca
    13201 agcaaaggat ttattgaatg gtcgatcaga tgcagaaact aaaaagattg ccttgattat
    13261 tagcagtatt cctggttggg aacggaagca actgccaaaa agaaataaac gacgaggatt
    13321 ttacaataag tcaaatgcct atgaaaataa aaatcgagct ggaaagtaca aaaagtaacg
    13381 ggtagtaacg ggtcagtaac acctagcccg ttactagttt acccttactg ccccaaggga
    13441 tacagcaatt tggtaacggg gtaacacctt atttccttaa aacttttgaa atagttagct
    13501 aatatgctgg ggaataatgt tactggattt tacccgttac ctgttaccaa aacgctagaa
    13561 cccttgtccc cgttgggatt gagtggtaac gggtacctgt tacgtaggtg ttaccacgtg
    13621 ttacgaatca aggaggaaag taaatgctca atattatttc aacaaacaaa gcaccgaact
    13681 ttcaatatac agatgaaatg gatcggtttc taatgaacac gctggcattt agtgtgggat
    13741 tggtaacaga agattattcc acatttgatc cagaagtatt aaagataatg gaagaagaac
    13801 ctgattggct gcaggagtca gtagcgtggt gtcaatcgct agtagtggga tcattggtag
    13861 atagtggcaa ctatgatgat acaggtgagc taatggatga atttaattgt ctgcttaacc
    13921 tatatgatcg agcgagacaa cgagagctta catcgaatga ggataatttg tttttaaaca
    13981 tccatgataa gttcttggct ttgctactaa cagatgatga attgataact aatttattgg
    14041 aggtagaata attatgcata tggaaagtat tagatacttc gatggtgaga aacacattga
    14101 gtattcaaac actcaagaag atgtagactt tattagtttt gaggaagata aaatcactaa
    14161 agttaatttt aaagacggaa cgtgcttgaa gatagtttcg ccttatattg aatacaaatc
    14221 aaaatgggaa caagatgatt ctgacgaacg agatcgcttt ggttggtaat ctatggcagt
    14281 taaaaaacaa tgtaatcatg ctggatgtaa aacattgatt gattataggc agaagtattg
    14341 tgggaagcat aaagcaaagc aaacggcaat taagcgtgaa gaaagaaagc aaagcgaagg
    14401 taaatatttt caattctatc aaagtagaac atggcgaaag gcttcatact tgtatcggtt
    14461 aaatcatcca gtatgtgagg attgcctaga agaaggcttg ataaggaaag ctgatgttgt
    14521 agatcacaaa attgaactaa aagatgattg gaatagacgg ttagatgaaa ctaattttcg
    14581 ttctttatgc catgcacacc ataattccaa gacagccaat gaaagacaaa gacgagaaaa
    14641 gagtaccctt tgagttaagg gggcatagtg tgaaacttac tgacaatcga tgcctactca
    14701 ttttggtaca aataaccgtt gtgaaaaggc ataagggtaa ttacaaataa cgattacact
    14761 tgtaatcaca agtgaaaaag tatataatga aagtaggaga tttattgaaa aatacaattg
    14821 ttattactga taaaacaggg aaacagcgca ccattgagtt acctcatttt ggtcaagtga
    14881 ccattcaaat gcagaacgga aaaatcattt atatagataa attagataaa gaaaaattct
    14941 gatcgaaaaa cgaaggaatg tgagcttaat tgcttacgtt cctttttctt ttgtctgaaa
    15001 ggaggacaac atgggacacc ctaaaatatt acacgataca aaaggcaata tatcaagtga
    15061 agaaaaagca atccgtgtgg acgctagaga agaattgttc aagcagcaac cattaataaa
    15121 tatcacgccc cctgactgga tggcagcgag cgctagaagt gaatggaatc gtatcgtacc
    15181 aacattaaaa aaagattatc cattgagtga agcagattat ggttcattgg tagcctattg
    15241 tttagccttt gctcgaatga aaacagccga agccgagata agaaaatcag gtacgtttat
    15301 cacatgcgaa aacggagtaa agaaagccaa tccagcggtt cgggttcaat cacaagctat
    15361 gagtgatttg aaaaaacaag ccacctcact aggtatgacc ttagaatcac gatcaaaact
    15421 agctttgaac aaggttaaaa atgatgaacc cgaagacccg ttcaaagagt tgatgaattc
    15481 atgaatgatt acattgagaa agtcctatca ggtaagctga ttgcacccaa gaaaattatc
    15541 caagcgtgtg agcgtcatat aagcgatttg gagcgttcta agtcagatag cttcccttat
    15601 gtgttcgatg aagaacaagc caccaaagcg attaaattca ttgagttgct accatctacg
    15661 gacggtaaag caatcaagat gttaggattt caaaaattca ttctaggcag tctttatggc
    15721 tggcgtacta aagaagggaa ttacaggcga ttcaatcgag cgtttaccag tatgagccgt
    15781 aagaatggga aaacgtatat cgcaagtggc atggctgcca atgcgttgat tatggaacaa
    15841 gaaccagcag aagcaaggca agtattgttt gtaagtaacg ctttgaaaca agctaaattg
    15901 ggctatgata tgctgtctaa ttcacttaga aacgtggtca agtctagtaa gtttttaaga
    15961 ccacaactga aaattatgaa ctctaagatt cagcacttgc cctctaattc gttcgctatg
    16021 gcactggcta gtgaaaccag cacgctagat gggtttgcac caacaaccgc aattcttgac
    16081 gagtggcacg aagcaaaaac tcgtaaaacg tacaacgtca ttaagtcagg aatgacccaa
    16141 caaaagaacg gcttattgtg tgttattagt accgctgggc ttgatttaaa cgttcctatg
    16201 tacgaggaat acttattgtt agatcgtgtg ctaaaaggcg aagaacaagc cgacaggtac
    16261 tttatagcga tatgggaatt ggacgatccc gaagaaattc acgatcaaga gaaatggatc
    16321 aaagccaatc cgatttttga aagtgaagaa atcaaaaaag taatgattcc aaccattcaa
    16381 gatgatgtga accttgcttt gaaacaaaat aaccttaatt ctgtattggt gaaaaacttc
    16441 aatttatgga gacaagcgag tgaggacagc tatatgattg ctgaagactg gcaggcaacc
    16501 gaagtagaac cacaagatat tacaggcaag cccgtttata tcggagtgga tttatctaaa
    16561 acagatgatt taactagcgt ttcatggatc gtaccgctag ataacggcga actttattgt
    16621 gattcacata gctttgtagc caccaaatat gggcttcaag acaaagaaaa gcgcgacggt
    16681 ttgccttatc gagaactaga aaaagctggt gagtgttcca ttactcaatt agaaagtgga
    16741 atcgtggact atgaccaagt atttcagttt attcaagatt tgattcaaga aaatgatttg
    16801 gaatgtatgg ggatttgtta tgacccgtat aacgctaatt cgcttatcag taaagctgaa
    16861 aaagccaact acccaatgtt agaagtaaga caaggaacga ttactctaaa cgttccgacc
    16921 cgaactttta gagaacaagt ttatgaaggc aacgttattc acaataaaaa tacgattctc
    16981 acccatgcag tgaacaacgc tattttaaaa acggataaca acggcattca gattaataaa
    17041 tcaaagaaca gtaacaaaat tgatccaata gctgcattaa tcaatgccta tgtgtttgca
    17101 atggattact tcaccacaac ggaaggagcg aaagcagaca atgaatttta tacaagtgaa
    17161 gaattttctt tctaattaca ttcataccgt tcttttactt cttggattgg tgtgtgtgtt
    17221 ggttgctatc accttactaa caaatgtcta ctatggcttg ttagcgctgg gcatagtgct
    17281 tattggggta gcggtcatgc taaatacaga acagaaggga ggttaaaaga tggcattttt
    17341 taaagcgaga caaaatacaa cgggagatcc tttcctacaa catgtggtat caatccaatc
    17401 ggatgattac accaccagct ttacaagcgt tcgtgcatta agaaatagtg atgtgtttgc
    17461 agccgttcga attattgcta gtgatattgc ttcaagtccg attcaattgg tcaaaaacaa
    17521 tatgccacaa gctgataatg aactggtgaa gttactaaac gagaaaccta attcagaaat
    17581 ggatggttgg catttaaagt ttgctttggc ggtcaatatg ttgctaaacg gtaatagctt
    17641 tgcagagatt aaacgcaacg gtgaaaaggt agaagaaatt cacttattac ctaactcaag
    17701 tgtaacggtt actcaattag ataatggtac gttgtcttat cagattggtg ataaaaaaag
    17761 acgtgtgaag tctagcgata ttttgcactt caaatatttc acccaagatg gtttaacagg
    17821 attaccacca ctttatgcgt tacgtgatga actgaaaatt caacaagctg gcaaccgcac
    17881 attgcacaat ttctttactc gtggtgtcag tgggtcaggt attttgaagg ttcataagtc
    17941 agatttagac ggatcagcaa agaacgcaat tcgtgaaaag tttgaagaag ccaacggttc
    18001 aagtagtgga gataatgctc ttagaacgat cattcttgat gaaacaatgg actacaaaac
    18061 attagaaata aacaccgatg ttttgaagct tatcaattcc aatgattgga acacaaaaca
    18121 aattgccaag gcgtttggtg taccgattga gcgtttaggc gttgaaaatg aacattctag
    18181 caccgttcaa agtaatctcc agtatatcca aagtacgctg attcattact ttaatgtgtt
    18241 tgtgagtgaa ttagatacga aacttgaaac caatatccgc tttaattccg atcagttact
    18301 agagactgat cctgaaacaa aagtaaaaaa tgtattggat caggtcaaag ggtcacttct
    18361 cacgattaat gaggggcggt cgaaaatggg gctacccccc atggatggtg gcgatcgttt
    18421 actagcaagt ttgaacttta cgtatttaga tacgttggag aaatatcaat taaaagaaca
    18481 ggaaggagtt acaccagttg aataatgaag aagaaaagga aaaacggctg acagaagaag
    18541 ctgagctaac agccgattct cccaaagtag agaaagaaaa tgaaaaacaa ccaacagacg
    18601 gcaaaactat ttcaggctat gcgttgaaat tcgggcaacc gtcaaaagat ttaggcggct
    18661 ttgtagaagt gattacaccc gaagcattaa aagaggtgga tttatcaaat gtgttcttat
    18721 tatatggaca cgattattca aagccactag ctagtgtcaa agcaggtacg ttgaaattga
    18781 atgtcgatga tacaggatta cattttgaag cagagctaac cgacacgacc tattcaaatg
    18841 atgtgtatga gaatatctca aaaggggttg ttgattctat gtcctttggg ttcgtgcttg
    18901 gattagattc attcgacaaa aaagaagatg gcacaattga acgatcaata gataaaatca
    18961 aagcacttaa tgaaattagt gtcgtgaccg ttcccgctta tgattcatca aatgtccaag
    19021 taaataagcg ttcttacgaa tcgtttatga gtaacaacca agcaaaacaa acaaacaata
    19081 gcttagaatc cacttctaaa gcacaaaagg agagtaataa catggaaaaa actttaatcg
    19141 ataacgaaaa aacagaattg cgtgggtatg aagaatatat ccgttcacaa ggcgaagtgc
    19201 gtgatggagt cactactgta aatgcagcgg cagttgttcc cgaagaagta atcggtgaag
    19261 tctttgattt gaaacgttca aattataact tagctcaata tgcaacagta aaaacagtat
    19321 caaatggaca aggtaaatat ccagtagcaa ctaaccaaca agcagtgtta gcaacaaaag
    19381 ctgaacttgc tgaaattggt gatattgacg ctgaaatgtt tacttcagtt gattataaag
    19441 tagaaactcg tgctggtaag attgccttat caaatgaggt tgtggaagat tcagcagtga
    19501 atattgtaca agaggtcaaa gatcagttag cgaaattggt agaaaacact gacaataagc
    19561 atatcatgga tttattaaaa acattcacta agaaaacggc tgctacgttg gacgatttga
    19621 aacaactata caatgtggca ttagacccag cattaaataa aatggtaatt ctaaaccaaa
    19681 gcggatatta ccacctagat acattgaaag attcagatgg acgttacatt ttacaacccg
    19741 atgtgacagc acctagtggt aaatcattat tcggtatgcc agtagtattg attgcagata
    19801 cattgtttgc caatcctaaa gctggaacat tccctatgat tatgggggat attgcacaat
    19861 ctatctttgt tgctcgtaga aatcaagtaa cgactcaatg ggaaaaattc gattactact
    19921 cacaaggact tgcagtgatc gttcgcaacg attataagaa aattgatcaa aatgcttcag
    19981 tgtatattga gtttacgcca gttgtaacac caaaagtata gaaaaatatt gggcggcggt
    20041 ttatcccact gccttttttt cattaggaga gataaaatgg taattttgga tagcataaaa
    20101 aaaagtatgc ggatcgatca cactattgat gatgacttta ttcaacaatt gattgataca
    20161 gcaggcgaat atataaaaag tgctattgat agtagcgcaa cggataaaga tatggataat
    20221 tatcagcaat ttgatttggc ggtgtcatta cttactcaac attggtattt gaaccgtcaa
    20281 gaagccagca gcgaacggat accagtaacg gtacaagcat tagtacaaca aatgagaggt
    20341 gcttattatg ccaatcatta agaatgtaaa tgaattgact gagagagtca agtttaaaaa
    20401 gacaaaacgg gtaaaagatg aagatgggca gatggtggat agtgaagaaa ctgtatttga
    20461 atgttgggct aatgtgcgtt cacaaatgct aaaagacgtg cttgctagtg taggtaccat
    20521 tcttgaagga acgttgacgt ttattattcg atacgatcaa gattatgaac taacaaacga
    20581 tatgaaagta gcttggaaaa acaagagtta taaaattatt tcaatcaatg aaggaacggc
    20641 gtttaaagat tatacaacga tcatagctaa acagatttct tagaatgatt acacttgtaa
    20701 acgttgtggg tatttagtat aattaaggta gtaaatgaag ggttagctgc ctgaattttg
    20761 caagacttag ctagtcgaaa tctattgttg gactgaaaat tgtaggtgtg gttgcaaaca
    20821 tgctggacta agaaagatga tagatgatga acgttcaatt ttaatacagg catgctagga
    20881 ctgattggat ggttttggca agctgattgt gtagaaatca aagaacacgt ctgttgtgga
    20941 cgtgtttttt ttgttataat tcaaaagagg tgatttaatg gacaaagcag agtttacaaa
    21001 tttttattgg aagtattatc tacatttaga aaatgagttt attaatacca cagattttgt
    21061 gatgctcgat gagaataatt tcaaaacatt ctctattgaa tatcaaaaac tactactagc
    21121 gattggttcg gagtgtgaga ttattttcaa agaactttgc ggatttgata gcagtagcaa
    21181 taaaagcata actgatttta aagctgttat taaatctagc gaattaatgt gtttggataa
    21241 tggtgttagg gttatgaatt ctttaacatt gacttcacta aaaccttttg gagatgggtg
    21301 gcctgaagat acaccagatt ggtggaaaat atataacaga gtaaaacatg gtcgttcttc
    21361 aaattataag gaagcaaatt tggaaaatgt gttgtatgct ttagcaagtc tatatttatt
    21421 agaggaatat ttgcataaga aagtaatact agaaggtgaa gtagacttta taagtccaga
    21481 gtcaaatcta tttacgttat cttgggaaag aaaacattcg tcgatgcaaa aagtgacgtt
    21541 tgaaaaagtg gattcaaatt atgtagctcc actcattgat tttaaagaac aagaagaaat
    21601 ttaaaaaccc ccacggctta ttgaagtttt tgtatgccat attgtatgcc atttggttta
    21661 aatcgcttga aaaatagtga tagataactc taattcattt agtcataaac gctgttttga
    21721 taacctttat agttctttga tatgggtttc tcattcatgg cggaagaaga agaatagaac
    21781 aaaagaatga attaataaga tataaaaaaa gggattgtga caaacgtctt gtcacaatcc
    21841 ctttttccaa ataaacgatg ttcaatcagc tgcccctcgg cattcgctcg aaaaacggcg
    21901 aaatatgaag aactattttg ccatttgttt ctctaataat caggtcaaaa cgttgacttc
    21961 acagtctctt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatattc
    22021 aacaaacatg agaagctgtt ttttgaaatt gttccttgtg actcggagct gaacggcagg
    22081 ctcacgacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagttcaacc
    22141 aaacgaaaat tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag
    22201 ctttttcaca acctcttttc gtttagcact aaatttcctt aaaaagtaga aaagctttcg
    22261 aagcctcgtt tgtttctttt actgtataat aaaagaaaag agtaacaatc gctaaaagat
    22321 ataaacaaga gaaaggaaat acaagatgat tggtctgatt tattttgtgg tgatcgttct
    22381 ggcgaacaca gttggcgcta tttccgggat gggtggaggc gtgttgatca aaccgattct
    22441 tgatcttatc ggcgcccatt ctgttgcagg gatttctttt tactcaacag ttgcggtatt
    22501 cacgatgtcc attgtttcga ctgtgcgaca agtatcaagc gggaaatcac tgaattggca
    22561 gattgtcggt tgggtatcta gtggagctgt tgttggagga gtagtcggca acattgtatt
    22621 cgaagttttt ttacagttat ttgaaaacga gaagcatgtg cagctgatcc aaattttttt
    22681 gacggtattg accttggttt ttgcgttctt ctacaccaag catcatcagc caaaatttca
    22741 tttgacgtca tggacttggt accttatatg tgggggggtt ctgggctttt tagctagttt
    22801 tttaggtatt ggtggtggac cagtcaatgt ttctttgttg atgctgatgt ttgcattgcc
    22861 aataaaagaa gcaacactat attctctatc tacgattttc ttttctcaac tggcaaaatt
    22921 ggtaacgatt gctttaacaa gtagctttat gcgctttgat ttatctatgt tgttttatgt
    22981 tatccccgct gctgttattg gaggtttgtg gggagcaaga tttagcaggg ttttgtctcc
    23041 aaaaaaagtg acctttattt ttcaagtaat tgtcctcgtg gttcttttgg tcaattgtta
    23101 caatgccttt gtcatcttac aaacattagg agaatctaga ctaacgcaag tcaaaatcac
    23161 tgaaacatat tgttctaaat gaaaaaagga gtatactgtg ataaaaaaat tcatttaaaa
    23221 aaataaaaat tggctggtga tttggatgtt gcgggaaatg cgtttaggag atgcagaagc
    23281 actacgagaa atcaatgcgg aacaattagg atatgatgta ccattaaccg ttacggtgag
    23341 acaaatgaag aaactgttga aggagccaga aaaaaatttt ttcttggtct atgaagatcc
    23401 tacttctaaa aaagcagctg gctatgtaca agcggagtcc tatgaatcga ttttcagcga
    23461 tccgatgttc aatattatgg cacttgctgt gtctaaacat gctgaaaaaa gaggaatcgg
    23521 aaaggccttg atgactggcg tagaagaagc gaaaagacga ggaatatcag caataagact
    23581 taattctgca gaatacagag tagaagcaca ccgattttat gaacgcatcg gttatcattc
    23641 cgataaaatg caaaaaagat ttttgaaaat cctgtaaatt tttatcaaaa aatacctggt
    23701 catcaagccg atacttgatg atcaggtatt ttttttgact aagaaagtaa ttgcatcaag
    23761 aaacctgaga tgatcaaaac gatggcacca ccaagccgat tgcccatttg agcaaaagca
    23821 ataagttcca tccgattagc tgcagataag acagctacat ttcctgtacc acccatcgag
    23881 ttgttgcata agccagcagt aatagttgat tcgattggat ataaaccaaa caaacgaccg
    23941 acaaatcctg aaactactga aatcgcgata acacttgtca aacataagac aacaaattgc
    24001 catgtcaaag cagaagccaa tacatttaga ttcaataacg caataccgat accggcaaga
    24061 acagctgctg tcaagttttt gacaatcaag ttgttgaaca tgatagctgc atcttcgtaa
    24121 tattctggta ataggtttgc gattttacag ataacaacga taatgatcat gaatgcgtaa
    24181 gcatggactt taggaacaaa atagttacag atttgtccta agatgaagaa agtcaatgat
    24241 accatcaaac cgacacctaa ttgtggaatg gaaggtttta catttctagg ttttccatcg
    24301 ccaaggtcgc ctttttccat taatttacca ttaccgttga attttggcat tgattgtccc
    24361 gcacgagcaa ctaacgctgc accaatgata gctaggacat ttgtcattgc tgtcgcagga
    24421 atcaggcggg aaatgataga agaagcgtcc gtacctaaaa catttgcata aatcgttgat
    24481 aaaggtacag ctccggctcc aacaccacca gccattgcgg gcaatgagat atataaaacg
    24541 gaatttgcaa aaccatttcc gataagcata cctacgagtc ctacggcaaa aaaggagacg
    24601 accattgaaa taagtgcaac gggaataaaa cgaacagaag ctttcatcaa tagattacgg
    24661 ttcataccaa ggatgctacc agtaatcaag gcagcaatat aaaagtctaa aaagcccata
    24721 ttgctgacaa agttttctgt ggattttact acatactcag gtaagatccc aaatgtagca
    24781 atcgcggcag aggcaaagat cgcaaaaacg gagccaccac ctaaataact tttgacgata
    24841 gggatttttg ttccgataaa atgaaataaa ttaccgaaaa tgaccaaaac agcgattggg
    24901 ccaatcatat tcgtcggtaa ttttcctaaa gccattgcaa tgatcaggac aaggaccatg
    24961 atcaaataaa ggggcaaact tacgccggta atttttgttg accaaaaccc ttcttttttc
    25021 ttttcctcaa ctgcagttgc ggttgttgtt ttttgtgcca tttttacaga tcctttctta
    25081 ttctgtatcg tatgaaaaaa gccaaagaga agcggcaaaa gagcagtagt tggctggctg
    25141 ctgttttgcc gcagtcgctt ttttcttttt agacgtcata caaagtttct gtatgttctt
    25201 ttactataac aatcttaatc ttctaaagat ttatattcag gaacccattt agcagattca
    25261 acagcttctt tgatatccgt gatttcttca cgattcaatt tttgtgcaac aactgatttt
    25321 gcgacagttt cagcaatcgt ttgagagaat tcagtgattt ttgctactgg aggcaagata
    25381 gctgctcctg gttttgttac atcaacgatt ccgcctaatg ctcgactagc ttgagaaatg
    25441 atttcagcat ttactcgtgt agctgttgaa gcaatcaaac caagccctaa acctgggtac
    25501 atcaacgcat tatttgcttg accgatttgg taagttacac cgttatattc cacatcagca
    25561 gcagggatac ctgttccaac taaagctttt ccgtccgtcc attcaatcaa atcttttgct
    25621 tttgcttctg ccaattttgt tggattagac aaaggcatga tgattggacg tggggtatga
    25681 gccgccattt ctttcacgat ttcttcagtg aatgctccag gttgggtaga agtaccgatc
    25741 atgatcgttg gatggattgc ttttacaact gcttctaagt ttgtcaattc atcagcatta
    25801 gtgaattctg agcgtttacg agcgaatggg atttgaccag gagtcaatcc ttttgtatct
    25861 tcaaacaata aaccctgttt atctacttga tagaaatgtt ggcgtgcttc ttcttcaggt
    25921 acaccttgac ggatcatttc atctaaaagg atgtttgcga tacctacacc agcagtacca
    25981 gcaccaaatg tcaagatcgt ttgatctttc aattgctcac ctgatatatt caatccacct
    26041 aatacaccag ctagtacaac gattcctgtg ccttggatat catcattgaa agtagtgatt
    26101 ttatcttcgt atttttctaa gatcgttgca gcatttcctc gtccaaaatc ttcccaatgt
    26161 aataacaatt ctgggaataa ttcagtagct gattgaacga attgatcaat gaattcgtag
    26221 taagtttcgc cttctacacg agcatgacga ttccctaagt ataatggatt attcaataat
    26281 tcttcgttat tagtaccagc gtcgattgat acaggcaaga cttgtgcagg attgatacca
    26341 gccgcagcag tgtagaccat caatttaccg atagcgatgt caacaccgtt cacaccccag
    26401 tcacccatac ctaagattcc ttctgcatct gtcacaacga tcaaacgaat gtcgcgtcca
    26461 tctgctgcgt ttttcaaact tgctttgata ttttctggtt cgtcgattga aagaaatgca
    26521 gcatcttgtg gtttcaagaa aatttcgttg tattgttcga ttgaatccgc aacgacagga
    26581 tcataaacga caggcatgaa ttcaactaaa tgttcgccca tcaatttata aaacaatgtc
    26641 cggttcgtat tgaatagatt cattaagaag atacgttttt ccaaatcggt ctgtttgctt
    26701 aagtattgac cgtaagcttg gacagattgt tgttccaatg tttggacagt acttggaagc
    26761 ataccagtga ttccgtattt tgcccgttct tcttttgtaa aagctgttcc tttatttaga
    26821 aaaggattat ttaataagtt tagccctgta agcatgaatg attcctccta agttttaata
    26881 caaacgaagt ataggagtca gatgaggata tagtcaaaaa gagcaggtat aataaatttt
    26941 atttatatga gtaatagagt caaaagaaat caaaatagcg gtatttgttc tttttatatc
    27001 aacaatcctt gcttgattac tatgcgtatc tcttatatgc taaagaaaga aaagtagaag
    27061 aataaaggat atgttagata gataaataga atctctgaag aataaatcac gtgatcgtga
    27121 cgataaaata cacttacgtc aggtgataaa aaatacataa gtagaaaact aagaggagga
    27181 acagaaaatg aaagaagttc aattgatcag aaaaagcgaa ctatcagaag gtggttgcaa
    27241 tgcatgcggc gtagtagaag caacaagcta tacattaaag cttggtgcca acaaagcgat
    27301 aatctctgag ttgacagtag gcggtcttgt ggacagtctg gcgcttgctg aaggttttat
    27361 tggagaagat atttatgaga tgttttcaga aatccgtcag ttaaaaaaag gggaaaactg
    27421 catagaggtt catcatgaga gcccgaatgt gagattcaaa cgtggtgata atgagatgat
    27481 cttcaataat catgtgtccg atcacaccga gctatatgga atcgtgaatc aaatactaac
    27541 tgagcttttt ggattgggac cttatgcgtt caaggaagaa aacggtaatc caaaattaaa
    27601 cgaagaatgg caagaaacga ttgaaataca aagaaacaac ccgcacttat ttcaataggc
    27661 atccacagta tttcgtaaaa gggactttga cacgactttt gtcaaagtcc catcgttttt
    27721 tatctatttg tgttctaaac atttctttca gagtagtaac aactggaatg actgaaatgt
    27781 aaacagatta tttgcctgta tcaatacgga agagtaatat aaagtgtgta aacgctatca
    27841 tattattcat tcaatttttt atagagggaa ggcattgaaa tggaaaaaac gtatcaagca
    27901 ggtacaaatg aaggaatagt ggattttatc aatatggaag atttagaaat agccgcttca
    27961 caagtgattc cagctggtgg ttatggttac atcagcagtg gtgctggcga cttatttact
    28021 tatcaagaaa atgaacgggc attcaatcat cagctgatta ttcctcatgt attaaaagat
    28081 gtagaattac ccgatacaac tacacatttt gacaaagaaa cgctgactgc accaatcatc
    28141 atggcaccag ttgctgctca tggcttagct catgtgaagg cggagaaagc ttctgcaaaa
    28201 ggtgtagcag attttgggac gatctataca gcaagttctt acgcttcttg tacattagaa
    28261 gagataagag aagcaggcgg ggagaaagca ccgcaatggt ttcaatttta tatgagcaaa
    28321 gatgatggga tcaatttgga tattcttgaa gtggccaagc gtaatggagc aaaagcaatc
    28381 gtattgacag cggatgcaac agttggcgga aaccgtgaaa cagaccgcag aaatgggttt
    28441 accttcccac tgccaatgcc gatcgttcag gcttatcaat caggagtagg ccaaacgatg
    28501 gatgctgtgt acaaatcttc caaacagaag cttagtccaa aagatgtaga atttattgct
    28561 gctcattctg atttgccagt ctatgtgaaa ggtgtccagt cagaagaaga tgtctatcgt
    28621 tcattagaat ctggtgctgg cggaatctgg gtatctaatc atggtggccg tcaactagat
    28681 ggtggtccag cagcgtttga ttcgttgcaa tatgtggcag aagcagtaga taaacgtgta
    28741 ccaatcgtgt ttgacagcgg tgtacgacgc ggacagcatg ttttcaaagc aatcgcatca
    28801 ggtgcagatc ttgttgctat tggcagaccc gttatttatg gattgtcttt aggaggaagt
    28861 acaggagtcc atcaagtgtt tgatttcttt aaaacagaat tggaaatggt catgcaatta
    28921 gctggaacac aaacagtaga agatatcaaa aaaataaaat tgcgtgagaa tcgttttatt
    28981 taagaaacaa atgacgaaaa aaataggagt gcggcagatg atgccgtact cctattttta
    29041 tagttcaagg atgtcactaa tagaataatt tgaaacgaaa aaataaaatt gaaatatttt
    29101 ttcatactta ataaaaaagg aagaatattc atgtaaacga ttttattgtt tttttctctt
    29161 ttatacaaaa agggtcataa tttcaagtaa gcggatgaat tcatgaggat tttttgcata
    29221 ctgtctctac tatacttagg tcactaagaa aaaggagtga tttattatga aaaagaacat
    29281 gattgggaca ttattacttt caacacttct tctaggcggc ttggcaacac cagcttttgc
    29341 tgagggtcaa gcgacttcaa aaggagatat tacctttact gaaccaacaa ataccgtgga
    29401 gcctcttaac cctactgatc cttcaaaacc agtagagcca gcagatccgg aaaatccagc
    29461 aactggccag acaggtcctt taacactgga tgtagttccc gaattgccat ttggtacaca
    29521 cgagattgaa agcggaacaa aaacgtatca agtagacgct tcaaaaaacg atacacctta
    29581 tctccaagta tcagatcgtc gaggtgtagg tgcagatggc caagcacaag ggtggaacgt
    29641 gacagtttcg gtttcagatt ttgtaaacgg ttcacaagtt ttgcaaggcg ctgaattaga
    29701 ttttggaaca tccactgtta aaagtacgtc agataacgaa tccactggac caactagtca
    29761 aactgtaact gggttgagta aagcaagtgc agcgacacca atatttacag cagcaaaaga
    29821 tcaaggtctg ggaacatggc tttctgttta tgatccagca aatatcacac tgaaagtgcc
    29881 gaaagcagca gctggaacat tcacggctga tttgacttgg aacctcgtgg ctggcccagt
    29941 agcatagagt atgaaataaa ttcaacaaac tgcggtgaaa atactatagg tgaaggattt
    30001 tttaccgcag tttttgttga aaggatgtgg tggacaatga atcaaagtaa acgactgttt
    30061 gtttcgtttt tttctatttt aagtctattt ttcatttttc cgtcaatcag ccaagcaaag
    30121 gattcagcag gagactttgg aatcaaaccg gtatttccag agaaccaaat agataaagca
    30181 atcggttatt ttgatttatt agtggcaccg gaacaaaatc agacactaga agtaattatc
    30241 agcaattctt cagatgaaga acgaacattt gaggtctctg tcaatccggc ggtaacgagt
    30301 gatggtggaa cgatcgatta tagtcaaaaa aacccaacgt tagatgagac attgccgttt
    30361 gatgtccgtg acgttttgct cattgcgaaa aaggaaatca atgtatcagc acatgcagaa
    30421 acaactgtac ctatagaagt gaaaatccca gcaaaaagct ttaagggacg tgtattagcg
    30481 ggtattcatg tttcacctaa agaagaagct gaaacagaaa atgctacaga aggagcacaa
    30541 atcaaaaatc gtattgctta taatctcgca gtcgttttac aagaaagtca agagacgata
    30601 gaaccggatc tgaaactttt atcaggagat ttggacgagg tcaacgcaaa gccaactgtt
    30661 cagcttcgtt tccaaaatcc tcagcctagg attatcagca atttgatatt tacgagcaag
    30721 atcttttatg aaaatcagct ttatattgaa aatacgtcta atgcattttt agttgcacca
    30781 aattcgaatt ttcacttgaa tcttgattta gctggtgata aagcaaaagc cggcgattac
    30841 cgcgcagaaa tcatagcaaa atcaggcgac agtaatgaat ggcgatttac acaaaacttt
    30901 acgatcaaaa aagaaaaagc acaaaaagta aatgaaaatt ccgtttttgc agtacaagaa
    30961 caatctttcc catggcttta tgcaggattg gttgctgcag ttctagtgtt atggttgatt
    31021 ttgctgtttt tgtgggtcca caagaaaaag aaaaaaggag aaacaacatg atgaggtgat
    31081 ttcgttgaaa acatcgatct tattgggagg gattattgca agtttgctgt tcttcagctg
    31141 gcaagagcca gtccaagcag ataccaaaag ttcaggtacg attacatttt atgatctaag
    31201 tccaattccg catccaagtg gttcagtcaa tggcgcatca aaacagcaga cacagctacc
    31261 gatttataaa gaatctaatc aacatggcgt attcccggag acgaatgaac aaaagatccg
    31321 ttttctttat ttgatcattg gtatagaatt atggcttctt gcgtggttgt tgttatggag
    31381 aagaaaggaa aaggaggatg aacatgttgc tacaaaataa attttcattt tttcgctcct
    31441 tcttttttac ttcgcttttt gttttaagta tatttatctt ttcaccttcg gcagaagctg
    31501 ttggagcaac gcctcctgcc atttcttatg atccatacac gtatgaaaat atcgtacctc
    31561 caaatggtgg acgtagtgca gtcaatggcg ggaagcttgt ccaactaact ggggctaacc
    31621 aattgcctaa attgactgct acttatttga acgatggttc aaatgatcct tggaaaggtg
    31681 gcacggtccg ttttcattgg gaaagccatg tgaaactaac aaatgtactt gatttggtga
    31741 atatcactgc acaaggattt gcatttgtgg cgatcatcaa tttgccggaa catgtatcaa
    31801 gcagtgatgt attaaaagca attgactgga acaacgccta cttgtatatt gccggtgccc
    31861 ccatcaaggt tactagcgga agtttattat cagcaggaag ccataccttg cgttttggga
    31921 tggggtcgtg ggatacaaat actctattga cagatattat tgctattatt acgcgaggcg
    31981 aattcaacgt ggacgatatc ccgcttcaat ttgatgttac agctaatgtc gcgaagatga
    32041 cagaaaacgg agataaatat gatacgagtc caaacaaaat tttgaccaag ggtaaattac
    32101 aaccttctat gtctaaaaag gccgtttttt ctgttgattt ttatgattcg aataatatta
    32161 tccaagatcg tatgttcaat tcgattttag ctccagctgc aggtagattt tatcctaaat
    32221 taactggcgg aaacttgaat ccaggagcat atgcattttc tgcaagtgca gagataaata
    32281 cgtggaatag ttacatctca ccaagtgatt caacaggaaa atataataca tttgctgata
    32341 ccacagaata cgtggatgga aataaccaga accttccagg ctctaccaat agtcggacat
    32401 tgacgatggg gctgactgaa ggacgttttg atcagttgcc atttagtcgc tttgatcgcg
    32461 tagtcaacta ttttacagga aaaaacgtta cacaaggagc aatactgact catactccag
    32521 caagcacaag tggattgaat cagacagcca atatcgtgta cagtgggaaa gatgcgcaag
    32581 gcggattgct ttctcctgtt gctttaaagg tattggagca gtacaagcta gacggaacgg
    32641 ttttaccaac aaacttaaca gccgatagtc agtggggaaa gctcctttct tctgcatcgt
    32701 accaagtcac ggccaattgg tcagcaactg aactaaaaaa gggttcgatc cattacaggc
    32761 tttattcgaa aggaacccaa aaattagcga caggatggac agatcaactt ttccaaacga
    32821 tccaatcaaa taacgggggt gtaaatacag ctacttctac actacctgca ttggcttcag
    32881 ggcaatatta ttttgattat cgcttggtgg acgatgtact tactaacatg tatccgtctt
    32941 ttgcgtacaa atggcagtct gaacaagctg gaatcaccac tcttcctcaa ataacagtgg
    33001 ctaattttcc atctgtgagt gcagatagtc agctaaaaaa ccttagcaga caacctgaga
    33061 caggagatcc cctgtctgct ctatcaggag atacgattca agaaaatgtg acattcacgt
    33121 taacaaaatc tggggatgcg ataaccgaca aaaaaatcac gatctcactt cctgaaaata
    33181 ctacttatat caatggttcc ttaaaattaa atggtacaac tatcgcagat accggcattc
    33241 aacaaggagt ttctgtccca gcagatctat tgagcaaaat aggagatacc attcatctta
    33301 cttacaacta tcaattaaat actgtagata cttctttaca atccgtcagc atactgacaa
    33361 aggcagctgt actttccagc aatattacat tagcagatgg tgctaagctt cctaacccag
    33421 ttgtccaaac atctgctaaa acaattcttg tccccaaaca agaattaaca ttagttaatg
    33481 taccagatga ttttacattt ggtaacgatc tccccaaacc gttgaagaca agttattatg
    33541 aatcaaaagg cgatttttct tttgatgtgc gggatactcg gcttccaagc acgtctcctt
    33601 ggcaactgac aggtacattg acttctttat ttaagaatga tcaaggtcaa gaattaagcg
    33661 gaacaaaatt atacttcaat cattctggat caaagcaatt gattcagcaa ggtcaaaaca
    33721 cgttgattta tgagagtgat ggaacagcta aaggagaggt tctcgttgac tttcccgata
    33781 ccgatggttt actcttagag gtaaatagca gtacgaatgc tcaacctggc gcaacgtatc
    33841 aaggcatggt cacatggaaa ctgacagctg ggccaaccag ctaagtgatt agaatttgta
    33901 tggatctgtg ataactgtca cagatcctct ttttcaagga tgagaaggaa acttctgctg
    33961 ttaaaatcag aaatgcttga aggagtggac ttggaacttt tcagtttgct tgaaaaaaat
    34021 gaacaacggc aaatcaaact tttccaactt ttattagagg aaaaaggaga ggtttccgtt
    34081 aataacgtac ttacccgctt atcagttgat cgggctacct tgaaagaaga tctgttttat
    34141 ttacaagcta acttggaaaa tatcaaacaa ttggaaattg ttatccaaga agggaatatt
    34201 ttattaatca gacatggaaa tatagcagca gtcgatgttt attatcattg ttacttgcgc
    34261 tattctacaa aatatcagat acttctttat ctgttggaaa atggctcatt tgatcggcag
    34321 aaacttgtca tggaacttaa tatcagttcg gctactttgt ctcgcagagt gaaggaatta
    34381 aatgattctt taaatgagtt tcatctgaaa ataaaaagcg ggcggctgac cggtccagaa
    34441 agtcaaattc gttattttta tttccaatta ttttggtttg gacgtcccta tcttgaaaac
    34501 aaaaaagaat acagtgattt atcaggaaac acttttttag atctgctgca aaaagagttt
    34561 agttttgcta ttacagaaga aggacgattg aaatttgaaa tatggctttc cattatgaag
    34621 aagcgaatgc gctgtacgaa aaaatccaaa ccagattata gcaatcttcc tgttggattg
    34681 tttcaaacga cacaattttt attggacctc catacatttt tgacccgtta cttctttcaa
    34741 tatgcctttc cttggaatca aaatgaaacg ttggtttttt atcttttcat gatcagtaac
    34801 tttacattag atacacataa ctcgcatgtc caggagtttt tgataagcga aaaatacaaa
    34861 gacgacgtag tagctcaatt gaatcgaacg tttaaacaat cgatagaaga atatttctct
    34921 ttttatgttt ttccagaaga gatttcgcaa aagattgcgt tgacagtgat gcaaaatcac
    34981 gctcattttg cctattttag aggatggatg aatatttttg gccaagaagg attaaaacag
    35041 cggttaactg aagtagaaga taaacaattc ctggttttat gtgaaagatt ggtcagacaa
    35101 acatttgcta ttgttgattt tactgaagta gacgaggaaa atgcccgctt tgaattattt
    35161 gctcgctatg catctatttt acggacgctg atcaagcagt tagaattacg tctgcaaatt
    35221 gcttatgatc ttccttatga aaggctaatg gcggatatcg tgaaagaaat catccaggaa
    35281 caattgctga atacgatcaa atatgattta gtcgaatacg agaaagaaaa gcagtatgat
    35341 atcattttga ctagccaatt gaaagagtac ccgtcacaga agtccgctaa agtttttgta
    35401 ttcactagca acgaatataa ttatgatttc ccctatttga accaattttt aaaagaatgt
    35461 tatttagaaa aattgaattt aaggatgaat aaaacatgaa aatcgtgatc attggtgctt
    35521 cccatgcagg catcacagct gccctaaatc taagaaaact tcaaccgaaa gcagaagttt
    35581 tactgatcga tgaagatcat aaagatggac taggctatgt ttcaaatgga atcaaccttt
    35641 atttaaaagg aaagatacgt agtttagctg aagttgccca taacatgcgg actcttcaag
    35701 aaagtggggc aaaactgatc accgaatgga gagtaactga acttgatcca gacaaacatc
    35761 agctcatcct cgcatccaaa gaaggaaaaa acgagaccat aacctatgat aaattaatcg
    35821 tagcgacagg ttcctctcct gtcaccttgt ataaacaaat tgaagctgaa aatgtctaca
    35881 cctataaaaa tctagtgcaa tctaagcaag tacttgccgc tttaaaagaa gcaaaagaag
    35941 tagtgatctt cggtgctggt tacattggat tggaattagc agatgctttg agaaataaag
    36001 gatacattat tcatctagtt gattatatgc cgaatgtttt atcaagatat ttcgataagg
    36061 atatgatcaa cagttttcaa aatcagctgc aaactaagca aataaatttt tatccaaatg
    36121 aattcttgat cgattggaaa aaatcagaag agaaagtagt atccgtacaa cttctgtctc
    36181 aaacaataaa ggcagatatg gtcatttttt ctgcccaaac tcgtcctaat acgacattgt
    36241 taaaagaaaa agtggctttg tatgaggatg agactgtgat agtcaatgaa tatctgcaga
    36301 caagtgatcc ggacatctat gcgattggag atatcgtccc agtcagtttt gacaaaaata
    36361 aacggcatct ctttttacct ttagttactc gagctgtaca tatggcaaga gcagtagcgc
    36421 taactttgag tggtcagcca accgcttacg acctcagaca aaagataaca gcagcagtca
    36481 ttatagacta ctttttagga actgtagggc tgacagaaga tgaagcgcca ttcttggaac
    36541 aaaataccgg ttcgtgttca ggagaatttg atctatttcc acaatacgac gaagaaaaca
    36601 aaacagtaaa tgcaaagctg atctaccatc cggatacatt agaaataatt ggcggacagc
    36661 tgataagcca agaattctta ttatcagatt tgaacctgct tgcggatatc gtgaaacaca
    36721 aaactacgat tccacagcta gcagttgagg attttggatt tctgtctgaa tacacctctc
    36781 gttttcacta cctcaatgag ttagctttta aagtactagc taagagagca aatagcaata
    36841 gagtagataa aagactgtga gaaatatcac ggtcttttat tgtgtgggga tataataaat
    36901 attaatcaat cgagaataaa aaattcatat aatgaaatag cggaagaatt ttttgagcat
    36961 aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt atttcaggag
    37021 gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac gttatgcgtt
    37081 agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg ggttagctta
    37141 tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt cgattgtgct
    37201 ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga tagcagaaga
    37261 agtcgaaggt gggaaagtgg cgtatcttgc ccaaacacct ggagtcgtga ctagtaaaga
    37321 cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa atcaattcat
    37381 catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag taggcgatat
    37441 tagtttgaaa gaagaatatg gtctagccat aggagataca gttaagttat ctggtagtga
    37501 caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct caccagttct
    37561 ctatacaacc atcaatgctt accaagaaat ccgcttcgaa aaagaagata caagtgaaaa
    37621 tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg aagatttaga
    37681 acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc aagtattgac
    37741 ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg ggattttcat
    37801 ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac aagggatcac
    37861 aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat ttgttggtat
    37921 tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg cggttccatt
    37981 ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgttagtag tagctgtgtt
    38041 aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg caattggata
    38101 agaaggagga agaacatgaa agcaatagaa tttaaagaag tagaaaaaag tttccaagat
    38161 ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa aggggaattt
    38221 gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt ggcaggcgga
    38281 ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag taatcaaaaa
    38341 gaaagcgaac gcgttaaact acgttttaaa gaaattggct ttattttgca agcttccaat
    38401 ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat taaacatgaa
    38461 catcgtcaaa aagaagcaaa cgaattattt gagcagcttg gcgtggataa gttgatcaac
    38521 aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc tcgtgcattg
    38581 tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga ttcagaaaag
    38641 gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa agcaacgatc
    38701 atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat gatggaagat
    38761 ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc atcgatacaa
    38821 caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt ccaagggata
    38881 tctcggcact tcaacaagag aaatcgcaca aaaagttggg atcacccagc ccaatctcta
    38941 tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc acttaaaaga
    39001 tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc aagtaacctt
    39061 gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc tgatgcttca
    39121 cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc tatggaagaa
    39181 aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc taagaaatgg
    39241 gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt acatcactcg
    39301 gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt tatatttgaa
    39361 tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaatagttag gtgaagaagc
    39421 aagatatgac gaagatcgtt atatcttgct tctttatata ggtagttttt cataaagaca
    39481 aatttgttat tctctcatga ttatcagggt atatttttat ttattatgtc ggtgttccag
    39541 gaagtgtttt aatttaataa cgaagaaatt aattgtttgt agaatgaagg aaactattta
    39601 ttgggtagat gaatcaccac gaatagtcaa aaggagattt caggatatgc caaaaatgaa
    39661 aaaaatcatt ttagcagtaa tgttattttc tacggttata tcttttacag cccctttttt
    39721 gcttacttaa gctgatttta agttttggcc cttagtctgg atctacgagg tgagagtcaa
    39781 gaatgaaaaa aataattgga gcttttgtat ttgcaatcat tatcattatt atctgttttc
    39841 aaatagatgt ttttggtcaa aaaaagatag ttggacaaac ggtcagcagt acgaacagta
    39901 cggttacaaa gacaaaggaa tcatttgatg atacagaaca agagacatta atcaaaatcg
    39961 gaaaaaatga ccgtacacta tatacagatg actcgctaac taccccactt gcaactataa
    40021 acggcggaga gctggcggag tttctttctg aaacaaaaaa tgcttatcag atcaagacaa
    40081 atgatggcta tactggatat ttagccctcg aagatggaac caaagtcaca aagaatatac
    40141 aaacaaaacc ggaagagcta tctgatgcag tgattgtatt agatcctggg catggcggaa
    40201 atgatactgg ggcattaagc aatgatgaac aaacagaaga aaaagatata acattaagta
    40261 cagccataaa agtaaaaaag gcgctagagg atgctggagc aacggtttat ttgacccata
    40321 ctacagatga attagtacag cttggagata tatgcgacta cagtgaagaa aaaaaggcag
    40381 atgtctttat cagtttacac gcagactcta cggaatatgc gaatgaagca actggaatca
    40441 cgacatatta ttactatgga caagaggaaa cattagcaca gacaattgct gactccttca
    40501 ctgatctgcc tctggattca agaggaatct ccactgggaa ctatcaagtt cttcgagaaa
    40561 atcttcaacc ttctatttta atcgagatgg gttatatgaa taatgatagt gatttaaagg
    40621 aattagtaac agacagttat cagcagcaga ttgctgcaag tttgacacaa gcattgacca
    40681 cttattttca gtaataaata agcccagcca aacaaaaata gctcctcata tttttcgttt
    40741 ggccgggctt attatcgcag atgcaagctt ttgaacaccg tttatttttt tagtagcttc
    40801 aaagcttttt ctgtatttgc aaagtgaagt ttggcatatt tttcaagcat aggcatgccg
    40861 cctttctcca atagttttgc cgctacttgg tcagatttcg ttccagctcc tgatggaagg
    40921 gtgattccag cttcagctga tgctttgtgt aactcttcca aaaagctggc acgactgatg
    40981 atagaagcag cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt
    41041 tcttgtaatt gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac
    41101 tgatcgatca aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc
    41161 gcttgattgt gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg
    41221 tatttttctg gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca
    41281 agctggatga tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat
    41341 tctgcaagct gatcacgttt cacataagca gcacatactg ttagcggacc gaaatagctg
    41401 ccatttccga cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa
    41461 gaggctgttt ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct
    41521 attgctgcat tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc
    41581 ccgtcttttt ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga
    41641 tagtatgctt tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata
    41701 aaattctcct cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa
    41761 gagattgcga gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa
    41821 atatttgtgg agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca
    41881 aataggagga aatgattcgt atggcgcacg aaaaaacacg ctacaaagcg gtgattgcca
    41941 accaaacata tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc
    42001 tgatcaatga acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag
    42061 cagcaatctt gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatct
    42121 tggagctgga agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg
    42181 aaaatcgtgt gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa
    42241 atggtcaagc agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga
    42301 aacgaaaagg acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa
    42361 aaatttaaaa aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct gattttattt
    42421 ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca aatcatattc
    42481 gcgattggat atgtattgtc atttattgct gcccagcatt tttacaaacc attagcaagt
    42541 catttggaat tatacattcc atacccagct gtcacgccaa catcaaagtt agcatttttt
    42601 gatcaggtat ttgcctttca cctagatgaa gctttttatg caggaactgc ttttctgatt
    42661 attttgctaa ttggctggct gcttacccga tttgtggggg tttttgtcca tggattgacg
    42721 tatgtgccga tcttgagaca ggtagactgg attgcaggag gcatcctgtc actaatcatg
    42781 gcttacgtta ctatattttt gattttgcag ttacttgcct ttgttccatt agatgtggtc
    42841 caaaatcaat tcggcgagca tagtttcgct cgctttatcg ttgagagaac gccatttctt
    42901 accaacaaga tgtatgagct ttgggtaacg aatattttga attaaggaaa atagtgctgg
    42961 aaaatatttc gttttttgta cagagtagat tatttaagaa ggctgcgtag cgtatgtccg
    43021 gcacagctct tttttcatag tgaaagataa ggtgataaaa tgaataaccg aatccttgaa
    43081 acattggaat ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac tgcacaagga
    43141 aaagaagaac tggcagaatt ggtaccagtg agcgaggaac aaacgatcac aaactggctt
    43201 caagaaactg aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc tatacctaaa
    43261 cttgaaaata tccgtcccca tatgaaacgg attgagatcg gagcagactt gaatggagtg
    43321 gagcttgcac aagtttccag agtattgtct acgacaagcg aattgaatcg tttcattgat
    43381 gatctttatg acagtgaaat cgaatttgca cgtttgtata tgtgggcaga tcagttagtc
    43441 acgatccctg ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg acgcgtgact
    43501 gatgacgctt ctcctgagtt aaagagcata agacagaaca ttcggcgaag tgaacaagct
    43561 gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg caaagtattt aagtgatgcg
    43621 atcatcacca tgcgaaatga ccgctacgtt atcccagtca aacaagaata ccgcggtgta
    43681 tttggcggtg tagtccacga tcaaagtgct tcaggacaga ctttatttat tgaaccaaaa
    43741 caagtggtgg atttgaataa tcgtttgcgt caataccaaa tcgctgaacg aaatgagatt
    43801 caacgtattt taagtgaact ttctgctgaa cttgttcccc atcgtcaaga aatcatccac
    43861 aatgcttatg tgattggtaa aatggatctg atgaatgcaa aagcacgatt tggtaaagaa
    43921 gtaaaagcta tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa acaagcaaga
    43981 catccactga tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg aaaagactat
    44041 caagccatcg tcattacagg acctaacaca ggtgggaaaa cgatcacgtt gaagacttta
    44101 ggtctgttac aactgatggg gcaagcagga ttaccgattc ctgcaggtga agaaagtcaa
    44161 atcggtattt ttgaggaagt atttgcggat ataggagacg aacagtcgat tgagcaaagt
    44221 ctatcgacct tttcttctca tatgacgaac actgtagata ttttatcaaa agtcaatgaa
    44281 aaaagtctgg tattatttga cgagctcggt gcaggaacag atccgcaaga aggtgctgct
    44341 ctagcaatcg caatcttaga tgatcttggt aaaaagtctg cttatgtgat ggcaacaacg
    44401 cattatcctg aattgaaagt ttatggatat aatcgggcaa atacgattaa tgccagcatg
    44461 gagtttgatg tggatacatt aagtccaacg tatcgtttgc tgattggcgt tcctggacga
    44521 agcaatgcgt ttgaaatctc tagccgatta ggtcttgata cagaagttat tgatgaagcg
    44581 aagcagttga tgaatgatga gagccaagat ttaaacgaaa tgatcacaga tttagagaat
    44641 cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc attttgtttc tgaggcacaa
    44701 gaacttcacg atgatttgaa agaagcttac agctatttct ttgaagaacg tgaaaaagaa
    44761 atggaaaaag caaagaaaaa agcaaatgaa gtcgtgtcag aggcggaaga aaaagcagaa
    44821 aaaatcattg ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa tgtgaaagag
    44881 catcagttga tcgatgcaaa gacccaatta gcaaatctac atcaagaaga aactttgaag
    44941 aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg agacgaagtg
    45001 ttggttacga cttacggcca aagagggacg cttttacgta aaaacgggaa tcagtggcaa
    45061 gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg agctgacgcc tgttgctcct
    45121 caaaaagagc cgacacaacg agttatccat gctgtacgat ctgaatctag tagtcacgtg
    45181 ccgaatcaat tggaccttcg aggaaaacga tacgaagaag cgctaagtga ggtagatcaa
    45241 tatttggatt ctgccattct agctggttat cctcaagtga ctattgtcca cggaaaagga
    45301 acaggcgcac ttcgcaaggg aattactgat tatcttaaaa accatcggag cgtcaaaagc
    45361 tttgagtttg caccggcaaa ccaaggcgga aatggtgcga caattgttaa atttaagtaa
    45421 agcttactaa aggtaagcag atggttcgct atccaagtat agaaatgcta tactagtcat
    45481 agattttaaa aatggaggtc tttgatatgt cacaagtaat tacagataag acatttaatg
    45541 aagaaacaga taaaggactt gtattgattg atttttgggc aacttggtgt ggtccatgtc
    45601 gtatgcaagc accgattttg gatcagttgg aacaagaata tgatgaagaa gaattcagaa
    45661 ttgctaaaat ggatgtggat gaaaatccag aaactcctca acaattcggt atcatgagca
    45721 ttccaacatt aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt ggtgttcatt
    45781 caaaagaaca actacgtcaa atgatcgatc aatatttata aaaacttaca gcaaaaataa
    45841 gctgagggtg ggactgaatc cagttccgtc ctcggttttt ttgcggttga gttaaggtta
    45901 ttcaaattaa tattttttga gtataattac gttgtcaggc tggattgttt ttttgacaga
    45961 catgctataa taactttaat tgaatatgaa ggggagtacc agatgaacga acgaatcaaa
    46021 aacaaattag ctttacttcc tgatcagccc ggctgttact tgatgaaaga taaaaatgga
    46081 acgatcattt atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc atattttact
    46141 ggtagccata atacgaaaac ggaacggctt gttagtgaaa tcgaagactt tgaatacatc
    46201 gttacagaat cgaatatcga agcattgctc ttagagatca atctgataaa gaaaaatgat
    46261 cctaaataca acattatgtt aaaggatgat aaaacctatc ctttccttaa gataacgaat
    46321 gaaaaatatc cccgtttggt gattactcgt aaggtactaa aagacaaagc actgtatttt
    46381 ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga tcgattattt
    46441 cctctgagaa aatgcaaacc tagtcagacg aaggagcctt gtctttacta tcatttggga
    46501 caatgtctgt gtccttatta tttcgacgta gatccgaaag tctatacgaa aatcgttgat
    46561 gaagtgaaaa actttttaaa cggaggacac gaaaaaatcg agtcagagat ccatcagaaa
    46621 atggaaaaag ctgctgaaaa catggagttt gaaaaagctg cagaataccg agaccagatc
    46681 cgtgccattg agacaatcat gactcgccaa aaaatgacaa atactgattt actggatcga
    46741 gatgtatttg gttatgcgat tgataaagga tggatgtgtg tccaagtgtt tttcgtaaga
    46801 caaggaaagc taattgaacg agatgtttca atgtttcctt tctataacga agcagaagaa
    46861 gatttcttga cttacctcgg tcagttttat caggaaaatg agcatttcat tcctaaagaa
    46921 gtcttgattc ctgatacgat cgacaagccc agtgtagaag ctttgttagc tacgaaagtg
    46981 ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat tggcgagtaa gaatgcctca
    47041 gttgctttga atgaacggtt tgatctgatt gccagaaaac aggaacggac gatcggagca
    47101 gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa tacgtatcga gtcttttgat
    47161 aactctaata tcatggggac gaatcctgtg gctgcgatgg tcgtattcat tgatggaaaa
    47221 ccggcaagaa aagaatatcg gaaatataaa ataaaaacag tcaacggacc ggatgattat
    47281 gcatcaatga gagaagtcat ctatcgcaga tattcgcgag tgattcgcga agggttgcct
    47341 ttacctgatt tgatcttgat cgatggagga aaaggacaag tagatgtggc aaaagatgtc
    47401 ttagaaaatc aattaggaat cgatattcct attgctggca tggcaaaaaa cgataaacat
    47461 aaaacaaatg agttgctatt cggtacagat ttggctgtag taccgcttga gcgaaattca
    47521 ccagagtttt tcttactcca gcgaatccaa gatgaagtcc atcgattcgc aatcactttc
    47581 catcggcagc tacgcagtaa aaacagtttt gcttcccgat tagatgaaat tgaagggtta
    47641 ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa tatcacagca
    47701 gccacgacag aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca aaacgtattt
    47761 gataaattac atcaggaatg aggaaagggc actttacaaa gaaagtaaag tgtcctatca
    47821 ttttatgtac aacaaaaaga gcgtttacgt tttgttggta aataataaat cgcttacgtc
    47881 tggtgtctaa ggatatatgt gatttcttga agaaggagtg aagaaatgtt agaagtcaag
    47941 gatcttgtta aaacttttgg gaactacaca gctgtcgatc atgtctcttt ccaaatcccc
    48001 gatggaaaaa tcatggggct aattggccaa aacggggctg ggaagacaac gacttttcgt
    48061 ttgatcttgg attttttgac acaagaccaa ggagaagtgt tatggaatgg ccatcatctt
    48121 ggggagaaag attatgatgt catcggttat cttccggagg aacggggact ttatccgaaa
    48181 gtaacgattc aagaccaact gctttatttt gcagaactta gagggaaatc aagaaaagag
    48241 attgaaccaa aaatcgactt ttggatggaa aaatttcagg taaaagggaa aaagacggat
    48301 aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc cacgttaatc
    48361 catgaaccta aattgatcat tttggatgag ccattcagcg ggttagatcc ggtaaatgct
    48421 gaattgctaa aagatggaat attggaattg aaaaaacatg gttcatgtgt cattttttct
    48481 agccacaata tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt gcgaaacgga
    48541 aaaatggttt tgaatggaaa agtacatgag attcgtgaat catttggccg tacgaagtta
    48601 ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt gaaaaaagtg
    48661 atccttcatg aagacaatac gttagagatt accttagaag atcctgaagc tgggaaagaa
    48721 atctttactc gtgcgactca gttcggctac attcctatgt ttaaccagca accgccaact
    48781 ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt ggattattac
    48841 caaagatgtc tatttaaaaa atgttaagtc gatttctttc ttgatcatga tattagtccc
    48901 ttttatcatg ctgggtatca tttatgtagc aggaaacttt gctcaacaaa atagtgatgt
    48961 caataaaatc ggcgtattgg ctgataatga gcaaatcagt cagcaaatga gccaagtcaa
    49021 aacagatgat tttgagttca aagcagttga ttctgaagaa aaagcacaaa aagagctttc
    49081 ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag aatgataaga taaatgggat
    49141 gctttatagc gaaaattcat taggacaaag tacacaacta ctgatacaac aacaactgac
    49201 tggcttgcaa tcgatgcttc gagcgagtca attaggcatt tcaccagaag aagtagctag
    49261 cttgagccaa caagcaagtt tgactcgaca aaaagtcaat tttgattcaa aaggaaaaat
    49321 gacagtcgga gaagataact cgacagtcca atacgtcgta agttatgtag caacgattgt
    49381 cttgttcatt attattttga cgtatgcgca gatcattgca caagagatcg cttctgaaaa
    49441 aggcacgcgg atcatggaag tgatcctgtc cagtacgact gcacaaaagc atttttacgg
    49501 aaaattaaca ggtgttttac ttgttgcatt aacgcaaatg gcattatacg gtattatttt
    49561 tggtatagga tacagtcaat tcaaagatat ggaaatggtc aaaaatatat tagacggtat
    49621 ctcattggat aacttgattg gaccattctt gtggtattct cttctattca tgatctttgg
    49681 cattttgatt ttttctgttt tagcagcttt atgtggctct ttggtcaaca aagcagaaga
    49741 tacagcaaaa gcaattttgc cagtgactta tctatctcta ggcggataca tgcttggatt
    49801 gatactagga gcttcagatc caaacaatat cgttattcgg atcacttcgt atattccatt
    49861 cttatcttca tatattatgc cggttcgctt agcaaatgag acagtcggta tgggtggagc
    49921 attgatttcc ttgttggtac ttgtagccgt aacattctta ttgatgatct tgtctgcaaa
    49981 catgtacaaa tcgaatgtac tcgtttatag cgaaggcgga ttatggagtt cattgaaaca
    50041 atcgatttca attatgagga atgaacgaaa aaaagcataa aacaatccta aaatacagaa
    50101 ggaagctgga catctattgt tcccagcttc tttttgtata gacctatttt atttatttta
    50161 ctatattgga tatatattca ttgaaaatag tgtataacaa ctttataata ttgagtatac
    50221 caatttttgg aggcgtttta atggaccgtt atttagattt actttcagaa aaattcccct
    50281 caacggagga tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag
    50341 gaacagagtt atttttgagt gatatacatg gcgaatttcc tgcttttgat catattctgc
    50401 gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttttctgaa caaatgacgg
    50461 aaacggatat cagccaattc actattttta cggcttatcc agaatatgca ttaacgactg
    50521 attggtacaa agaacaagat aaatcgcagc tgatccatca tttgatcgat cttttacggt
    50581 ttacgacagt caaatatacc cggtcaaaag ttcgaaaagg attgccgaaa gaatatagct
    50641 atatcattga ggaactcttg tatatcgatg accgtatcaa tggaaaaaaa gactatgtaa
    50701 aaaaaatcat tgaacaactt gttttgatga aggaagaaga aaaattttta acgaaacttg
    50761 cacagacgat ccaaaaatct gtcatcgatc atttgcatat cgttggagat atttttgacc
    50821 gaggaactca agcagccaaa gtgatggatc gaatcatgga tttttcttct gtagatatcc
    50881 aatggggaaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttac
    50941 tgacactttt acggattgca gcacgctata attatttatt tgaattagag aaagaatatg
    51001 ggttgaattt acgtccattg ttttcttttg ctcatcaaac ttatcagaag aatccagtat
    51061 ttgcgcctaa aaacagtaaa aatctatccg aaagagagca agaagaatta gaaaaaatcc
    51121 atcaggcact ttctatcctt caattcaaat cagaacatca gttattggat cgtcgacctg
    51181 aatttaaaat ggacgatcgt aaattgctgg aaaaaatcga ctatgaagca tcaacgattg
    51241 atttagaagg tcagctctat gagttgaaag gaacttgttt tcaaacgatc cagccagatg
    51301 atccaaaaaa attcttggaa gaggaacaaa aagtgattga tagtttgatg tattcattcc
    51361 agcactcttt gagaatgcaa aaacatatga cgtttttgat cgaaaaaggg ggaatgtatc
    51421 tcacgaacaa tcagcatgtg ctgtttcatg gctgtatacc tgtagacgag aagggacagc
    51481 tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctgggc ttttttgaaa
    51541 aacagataac tgaaagcgca aaagatctga ctgcaaaaga agatttagca accgatttga
    51601 tttggtatgc gtggagtggg ccattttcgc ctttattcgg caagagtaaa atggcgactt
    51661 ttgaacgtta ttttctcaca gagcctcata cgcacgtcga gaaaagcaat ccttattatc
    51721 acttgcgaga tgaagagtgg ttcagtgaaa aaattttagc agaattcgac acaaaagaag
    51781 aagggtcagc aatcattaat gggcacacgc cagtaaaagt aaaaaaaggg gagtcaccga
    51841 tcaaagcaaa tggaaaagta tttgtcattg acggaggttt atccaaagct tatcaaaaaa
    51901 cgacaggaat tgccggttat tcactattat gcaattctta tggtttccaa atcgtgacac
    51961 attatccgtt tttgcctatt gaacaacaat ttgcaaaaaa atccgaccaa acaaatgtga
    52021 aaaaagtaat cgaacagcaa ctaccacgca aactcaatcg tgatacaaca aaaggtatgg
    52081 aactgcagca gcagattacc cagctcaaaa ggctgttgga ctatcgaaag gacgattgag
    52141 aagaattcca gtgaacattg ttccatagac tggagactga gacaaaagtc gtgtcgcagt
    52201 ctctttttcc ggataaacgg tgttcttctg ccaactcctc ggtcacaagt cacactattc
    52261 aacaaacatg aaaagctgtt ttttgaaatt gctccttgtg actcagagtt gaacggcagg
    52321 ctcacgacct cttggtataa acggtgttca aaagccagtt tctcggtatt aagccaaatt
    52381 tatgcaaaaa tgtgaaaagc cattttcaca aaatttttct taatactcga aactaaacag
    52441 ctttttcaca accccctcag aaagtctatt aaaattcatt cacaaagaaa tcgaataagc
    52501 gctgttcaga tggatgtaca cggtgggtaa gttccggatg ccattgaaca ccgaggattt
    52561 tatgcatatc atctttgctt tgaaccgctt caatgagtcc atcgttagaa aaagcgatgg
    52621 cttttaataa aggtgaaaga tctttgactg cctgatgatg gtaagaattc acaaaatatg
    52681 gatcggggag aaggttgctt aacaaactgt ctgaaacaat cttgacttcg tgagtcgtaa
    52741 attgcggggc agtgggctgt tgttcatgtt ttactgacca ctttggatat aatgaaagat
    52801 cttgataaag tgtaccttcc aaaacgacat tcaatagttg gagtccccgg cagactgcaa
    52861 aaattggttt tctttgtttc aatgcttcca agatcagtgc ctgttcaaat tggtctcgtt
    52921 gaatatttgt ttccgttagc tttggatgag gatcttccat gtaaagttgt ggactgacat
    52981 cttgaccacc tgcaagaata agtttgtcga tttgtccaat cagttgagga gctgaatctg
    53041 gagacatgac tggcagaata agaggcaatc cttgagcatc taaaactgca tccacgaatc
    53101 cttgcggtgt atagctcact tgatttcctt ggaaagtatc tgttgctcga atcagttgat
    53161 ttccggcaat gccgatgatt ggtttcattt gtattccccc taaagtagac ttattgtaac
    53221 aaggatttaa aaaaaattaa actatgtaag acttgcccct atacttatct gttcttgctg
    53281 tgttactatc tgtaaagaga aatttcgtgt gaaattcatg acgatagttg aaaatattgc
    53341 ttaatttgtt ataataaaaa gtaagcagaa ataaaaaggt tgggcgtaag cttcaaggat
    53401 ctagttcttc aaggggtgag aaggacattt tattgaaggg gcctgcctat actaagttat
    53461 ctttagaggt acatactttc aatgaaaaaa gaaagcatgt acctcttttt taaatcgaaa
    53521 ggaagtgaag caagtgttag aagctttgga acaaatccga aaagcagaag aagaaaatga
    53581 aaagcgtgct caaacactgc ggcagcagct gaagatctat gcaacagaaa aagagcagga
    53641 attggaaacg atccaaacaa actatcgaca acaactaaaa cagttggtgg aagaaaaaga
    53701 acaatttgaa atacaagaat tagaaaaaga aaaaatgaaa ttgaaagcag aagcagacaa
    53761 ggtgcaagca actttgaaag atcaatatca aaagcatcag agtcaagcga tcggtgcgat
    53821 cattgaaagg gtgaaagaaa cttatggccg tcactaagat ggaaaaagtg accctcattt
    53881 cagataaaag aaatcaggag aggattttac aagcagtaca aggaatccaa aacgttgaaa
    53941 tccgggatct gtatcaaaat agaacaaaca accagtgggt ggagaagtat tttccagata
    54001 ccacgattat tgataaagaa gcaaaattaa gcgctttagg taatcggttg aaagacatcc
    54061 aagaagctat ccaattcata caacaccacg gcgatagccg tcaaaagaaa ctccatttga
    54121 aacgtcaaga actttcctta caagatttgg aagccaatta ttccgaagaa aatttcataa
    54181 ataaattgaa agaagtcctt gatttaaaag aaaagtggga acaactaact gaagaacgtg
    54241 atcgtttaga agaagaggaa gaatggcttc ttcattggca acagctggat attgctccaa
    54301 gtatgtatgc aagtaagacg accatttttg agatggggac tgtgagtgca gcaaattttg
    54361 aaagtttcaa ggaagaactg agtaagcttg atgacgttta tatggaagaa atagattata
    54421 cgccaaaaca agtttatctt tcgtatgttg ctttaaaaaa atcagttgct gttatagaag
    54481 aaattgctgg gaaatacggg ttcaataaag aaacgtatcc ttatgaacaa aatcctaaag
    54541 aaaaactgca agagaccaag cagcaattat ccattgttca cgagcagcaa aagaagataa
    54601 cgacagcgtt aggtcagtgc agtggctata tccaagaatt tgaatgggta gaagaagtca
    54661 cacttgccgt tgcagaacga gaaaaaataa aagaacggtt tatccatgcg acttatctga
    54721 tcgttttgca aggttgggta gatgcagaag aaaaacagaa tttgctgcat gtgttaaacg
    54781 aaaccgtatc agaagaagat atatacgtca gtttcgaaac accaaactca gaagagattc
    54841 agcaggaagt ccctactaaa ttgaaaaacc atccgcttgt cgaacctttt gaattgctga
    54901 ctgaaatgta cagtctgcca aaatatgaag aagtagatcc aacaccttgg atgacaccat
    54961 tttatcttgt cttcttcggg atgatggtcg cagatgttgg ttatggtttg ctgatgctca
    55021 ttgggacaat actagctcaa aaactacttg ttctaccacg cgggatgaaa cgtttcgtaa
    55081 aattctttga gattctctcg ataccatcga tcatctgggg attgatctac agttcatttt
    55141 tcggacagtc attaccaaaa gaactgtttg gcatccgctt gcctttccca atcctatcta
    55201 cgacggatga cgtcaatacg attttgattt tatccgtgat tttcggtctg atacagattt
    55261 tggttggatt atttgtggca gcaaaggaaa atatcaggag aaaagattac ttgagtgctg
    55321 tcagtgatgg atttgcctgg caagggatcc taattgggat cgttattgcg ttggttggtg
    55381 ccatgttgat aaaaaataca gcatttgttt accttggcgc tgggctatcg atcattgctg
    55441 ccttatgtat cttgatcgtt ccgattatcc aaacgccgtc taaagtcaaa ggtgctgcca
    55501 aaggtgcgta taatctttac ggactgaccg gctatatcgg tgatttagtc agttatacac
    55561 gtttgatggc attgggtatt tcaggcggaa gtattggagc ggcattcaat atgctagttg
    55621 ctttcatgcc gccagctgct cgattcagtg tagggatcct tttgatcatc gctttacacg
    55681 cactgaatat gtttttgaca ctgctaagtg cctatgtcca tggcgcacga ttgcagtatg
    55741 tcgaattttt tgggaagttt tatacaggag gcggacgtgc ctttgatccg ctgaaaacgg
    55801 cggaaaaata cgtcaacatc aatcacaaga aaaaaaacaa taaataattg gaggattttt
    55861 atataatgat ggattactta attagtcaaa atggtggaat ggtatttgcg gtattagcga
    55921 tggcaacagc gacgattttt tcagggatcg gttcagctaa aggtgttgga atgacaggtg
    55981 aggcagcagc agcattgaca acaagtcaac ccgaaaaatt cgggcaggct ctgattctgc
    56041 aattgcttcc aggtacgcaa ggtttgtatg gattcgttat tgcctttttg atttttatca
    56101 acttaggaaa tgacatgtca gttgtacaag gactgaactt tctaggtgct tctctgccga
    56161 ttgcttttac aggcttgttt tcaggtattg cccaaggaaa agtcgcagcg gcaggtattc
    56221 aaattttagc gaaaaaacct gaacatgcta caaaaggaat catttttgct gcgatggttg
    56281 aaacatacgc gattttaggt ttcgttattt ccttcctatt agtgttgaat gcctaagcat
    56341 aaaggaggaa aagcagatga acgccattga aaaaatcatc tctcagatga acgaagcagc
    56401 agagcaggaa agagcggcgt tagaacaaga agagcgaatg aagatcgatc aaaactttga
    56461 acaaaaacgt acgcaagttg aaacagaaca tcaaaaacaa aaagaaaaac aaatcgaatt
    56521 actcgaaaaa aaataccgtc agctaaggaa tcgccagcaa gtagaagtac gacaagaaaa
    56581 tttgaatgcg aagcaggaat ttttaagacg attgtttgca gatgcggtaa ctgaaatgga
    56641 aaattgggat gaatctgaac aaatccagtt tatcaaaaat gcattgtatt cattaccttt
    56701 gacaggaaaa gtagcattca ttgctggaga aaaatcagca gcctatcttt ctcagacgtt
    56761 gttggatgaa tggaacaatg aacttccctt tatgatggtt ttaagtgatg aaactgttgc
    56821 tgatcaagcg ggttttctga taaacgatca aggagttcag tataattttc ttttcagcag
    56881 ccttgtacaa gatatccaag gaacgatgag ttttgagatt gccaatcaat tattcgaata
    56941 aggagggatc agtgtatgga ataccacgaa ctaaatccct tgattcgcgg aagagagctt
    57001 gaacttttgt ccaatgagac atttgaacgg atgattcaaa caaattctgt cgaagcactt
    57061 ggtgaaatat tgaaatccac gatttacagt ccatatattt atgacggata tgaatatgac
    57121 ttcgaacaga atcttttaaa ggagcagagc cgtctatttc gctggttaaa ggaatcagca
    57181 ccggagcctg aaatcgtttg gatctatacg atgcgttata ctttccataa tctgaaggtt
    57241 ttgacaaaag ctgagatgac aaatcaaaat ctggaccatt tgtatatcga cgatggattt
    57301 tattcattag aaaccttgaa ggatgccatt catacgcaag tttcaaatga acttcctgaa
    57361 agcattatgg acagtatacg agaagtacgt gattattttg aagagtctac cattcttcag
    57421 ggaattgacg tgatttatga tcgacagttt ttgacagagc agcgacggct tgccgataag
    57481 ctaggatatc aagaattgct gacagaagtc attgctttta ttgacttgac caatatcaca
    57541 acgatggctc gaggaatatt gcagaaaaga acatatgggt tcatgacgac ggtactttcc
    57601 agctcgggaa gtatcgaaaa agaagtattt ttgaactttg tagataaaga tatggaagat
    57661 tacacgaatt ttctattaga gacagattat gctgattttc tgcgccctgt catccgtgag
    57721 ggaaatattg atctcgtaaa gcttgaacag ttgaaagatg attatctaag ttcattgtat
    57781 caagaagcac aaacccaggc tttcggacca ctgccactac tagccttttt gaacgcaaaa
    57841 gaggtagaaa gcaaaaacct tcgtttactg gttatcggca aaagaagcca tttcgaatca
    57901 gaaacaatca gaaagcgggt gagacaagtc tatgacgcat aagatcggtg tagttggcga
    57961 caaagattct gttttgccat tcaaattgtt tggctttgat gttcgttatg gtacaaccaa
    58021 gcaaaccgtt cgtcaggcaa ttgaagaaat ggcaaagaat gagtatggcg tgatttatgt
    58081 cacagagcaa tgtgcagcat tgagcccaga aacgatcgaa cgttacaagg aacaaatgat
    58141 acctgctatc gtattgattc ccaatcatca aggatcatta gggatagggc tggaagaaat
    58201 tcaaaaaaac gtagaaaaag cagtaggtca aaatatctta tagtatgaaa aggagataga
    58261 aattttgcaa attggaaaaa tcataaaagt ctccggacct cttgtaatgg ctgagaacat
    58321 gtctgatgcc agtatccaag acatgtgtct agtgggagat ctaggagtta tcggagaaat
    58381 tatcgagatg cgtggcgatg tggcgtctat tcaagtatat gaagagactt ctggaattgg
    58441 tccaggcgaa ccggttcgct ctactggtga agctttatct gttgaattag gaccagggat
    58501 catttcacaa atgtttgacg gaatccaacg gccattagat acctttatgg aaatcacaca
    58561 aagtaatttc ttagggcgcg gagttcaatt gcccgcattg gaccatgaga aaaaatggtg
    58621 gttcgaacca acagtagaag ccggagaaac ggtgagtgct ggagatgtta tcgggatcgt
    58681 agaagaaaca aaagttataa aacataaaat catggtgcca aatgggataa aaggaacgat
    58741 caaaacaatc gaaaaaggtt cattcactat tgatgaaaca gtatgtgtca ttgaaaccga
    58801 aaatggtgac aaaaatttga cgatgcttca aaaatggcca gtaagacgta gtcggccaat
    58861 caaagaaaaa ttaaacccgg atgcacccat gatcactgga cagcgagtga ttgatacatt
    58921 cttcccagtg acaaaaggag gagcagcagc tgtacctggt ccttttggtg cagggaaaac
    58981 agtcgttcag catcaaattg caaaatgggc cgatgtggac atggttgtct atgtgggatg
    59041 tggcgagcgt ggaaatgaga tgacggatgt tttaaatgaa ttccctgaat tgattgaccc
    59101 taatacagga gaatcgttga tggaacgtac ggtattgatt gctaatacat caaacatgcc
    59161 agtagcagct cgagaagctt ctatctatac gggaatcaca attgcggaat atttccgtga
    59221 catgggttat gacgttgcta tcatggcaga ctccacttca cgatgggcag aagcacttcg
    59281 tgagatgagc ggacgtttgg aagaaatgcc tggtgatgaa ggttatccag cttatcttgg
    59341 ttcacggctt gcggaatact atgaacgatc tggaagagtc attgcattag gatcagaagg
    59401 tcgcgaagga agtataaccg caatcagtgc tgtatccccg tctggagggg atatttcgga
    59461 accagtcaca caaaatacat tgcgggtcgt aaaagtattc tggggtcttg attccagttt
    59521 agcccaaaaa agacattttc cttcgatcga ttggatacaa agctattctc tttattcaac
    59581 agaagtaggg aagtatatgg atcatatcct tcaagctgac tgggcatcca tggtcaccga
    59641 gggcatgcgt attttgcagg aagaacagca gctgaatgaa atcgtgcgtt tggtagggat
    59701 tgattccctt tcagataatg accgtctgac attagaagtg gcaaaatcca ttcgtgaaga
    59761 ttatttgcag caaaatgctt ttgatgatgt cgatacattc acttcaagag agaaacaatt
    59821 caaaatgttg aagatgatct tgacctttgg tgaagaagga cgccgtgcgt tgtcgttagg
    59881 tgcttattta agtgagatca tggacggtac cgtatcgatt cgtgaaagga tcggacgaag
    59941 caaatatatc ccggaaacag aattagaaaa actagatcaa attattggtg aaatcaagca
    60001 aacgatgcaa acgatcgttt cagaaggagg catgagcaat gattaaagaa tatcgtacga
    60061 tcggtgaagt cgtcggtcca ttgatggcgg ttgaaaaagt tgctggtgtc aaatatgaag
    60121 aattaatcga agtccgtatg caaaatggcg agattcgtcg tggacaagta ttggaagtcc
    60181 aagaagataa agcaatggtc caaatattcg aaggaacaag tggtatcaat ttacgtgact
    60241 cttctgtacg atttttaggc catcctttag aactaggtgt atcggaagac atgatcggtc
    60301 gagtattcga tggtttggga cgaccgaaag ataacggacc agagatcctg cctgaaaagc
    60361 atttggatat taacggagaa gtaatcaatc cagtagctcg ggattatcca gatgagttta
    60421 tccaaacagg tatctctgca atcgatcatt tgaatacatt agttcgtggg caaaaattac
    60481 cagtattttc tggctctggt cttcctcata aagaattagc tgctcagatc gcgcgacaag
    60541 caacggtact cgattcatca gatgatttcg ccgtagtctt tgctgctatt ggaatcactt
    60601 ttgaagaagc tgaatttttc atggaagatt ttcgtcaaac gggcgcaatc gaccgttctg
    60661 tcatgtttat gaatttagca aacgaccctg caatcgaacg tatcgctact cctcgtatgg
    60721 ctttgactgc tgcagaatat cttgcttatg aaaagggaat gcatgttttg gttatcatga
    60781 cagacatgac caactatgca gaggcattgc gagagatttc tgcagcacgg cgagaagttc
    60841 caggaagaag aggatatcct gggtatcttt acacgaactt agctacttta ttcgaacgag
    60901 caggaagaat ccgcggacta aaaggatcgg ttactcaaat tccgattcta acaatgccag
    60961 aagacgataa aactcatcca atccctgact tgactggtta tattacagaa ggacagatca
    61021 tcttgactag agagctttac aagagtggga ttcagccacc aattgatgta ctcccgtctc
    61081 tttcgcggtt aaaagataag ggaactggac ccggaaagac aagagaagac catgcagcta
    61141 cgatgaatca gctttttgca gcctatgcac aaggaaaaca ggcaaaagaa ttagccgtag
    61201 ttttaggtga atctgcttta tctgatgtag acaagatcta tgcgaagttt gctgagcggt
    61261 ttgaaaaaga atatgtcaat caaggattct acacgaaccg ttcgattact gaaacacttg
    61321 atctagggtg ggaactgcta tcgatgttgc caagaaccga attaaaacga atcaaagacg
    61381 acatgctgga caaatatctg ccagaaggga agtgagttct gatggcacga ttgaatgtga
    61441 atccgacccg catggaattg actcgattga aaaaacagtt gacaactgcc acaagagggc
    61501 ataaattatt aaaagataag caagatgaac tgatgcgtca attcatcctg ttgattcgaa
    61561 agaacaacca gctgcgtcaa gaaatggaag agtctatgac agaagcaatg tctgattttg
    61621 tattggcaaa tgcttcgatc acggaacctt tcatcgaaga attatttgtt ttgccggcgg
    61681 aaaacgttga tttgtctatc gtagacaaaa atatcatgag tgtaaaagta cctgtgatga
    61741 attttgaata tgatgaacag ctgacagaca cacctttgga atacgggtat ttgaattcca
    61801 gtgccgaact ggatcggtca atcgatcggt ttactaccct gcttccaaaa cttttggaac
    61861 tgacagaaat cgaaaaaact tgtcaactga tggcgagtga aatcgaaaag acacgtcggc
    61921 gagtcaatgc attagaatat atgacgatcc ctcaattaga agaaacgatc tattatattc
    61981 gtatgaagct ggaagaaaac gagcgcgcag aagtaacaag gttgatcaaa gtaaaaaata
    62041 tgggtaccac tgactaagtg gtatcttttt ttaaaagagg aaaacgatga ttaaacaaag
    62101 caaacaaata aagaaacgtt tgccggctgt tcagcttctc gcattaggat tttttctcct
    62161 gattttagtt ggcggcggcc tacttaccct accatttttt agtaatagtg ggcatgggac
    62221 aaattttatc gatgcgcttt tcactgcaac ttctgctgtc tgtgtaacgg gattgacaac
    62281 attgaataca gcagagcatt ggaacagtgc tggtcaattt ttgatcatgc ttttgattga
    62341 gatcggcggt cttggtttta tgatggttcc gattctatgc tttgcattga taaagaagaa
    62401 agtcagtctc agcacacgta ttatattaaa agaagcgctg aatctagaag aagtatcagg
    62461 tgtgatgaag ctcatgcttt acatcctaaa attggccttt gtgatccaat tgattggtac
    62521 ggttgcttta tcttttgtat ttgtgcctga atttggctgg ttgaaaggta tctggtacag
    62581 tttgttccat gcaatctcta gtttttgtaa tgcagggttt gatttgttag gagacagttt
    62641 agccaatcac cagcaaaatg tttatttgat catggtggta tctgctttga tcatttctgg
    62701 tgggttaggc ttcatcgtat ggcgtgattt actggaatat catcagaaaa agaagatgac
    62761 gattcattca aaaatcgcat taaccgttac aggtaccctt ttattaggcg gattcgttgt
    62821 gttctttttc acagagcgca atggggcaac gctagtggaa gggaatatgt ttcaacgatt
    62881 ggcaaacacc ttttttatga gtgtgacacc tagaacggct ggatattatt cagttgatta
    62941 cctccatatg agccatgcag gtctgatttt aacgatgttt ttgatgtaca tcggcggaac
    63001 atctgggtcg acggccggcg gattgaaaac aactacgtta ggtgtgttgc tgatccagat
    63061 gcgttcgatg ttgaaaggac gtacgagagc ggaagcattt ggccgtacca tccgacaagg
    63121 cgcagtgttg cgggcactta ctttattttt tgttacgttg acgttgtgtg tagtcgccat
    63181 catgatttta tcggtgactg aaacgatacc taaaacttct ggcatcgaat atattgcttt
    63241 tgaggtcttc tctgcgtttg gtacagtagg tcttacgatg ggattgacgc cagacttgac
    63301 tgcaataggg aaattgatca ttatagcgct tatgtacatt ggacgagttg gtgtattgac
    63361 tgtggtcttc tctctgatga caaaagcaca aagaacacaa tcaaaataca aatatccaga
    63421 agaaagtgtc atgattggat aatttcatct tctgacagaa acctagtgat ttagtaaaat
    63481 aagaaaataa aataattttt cttgcttctt ggatacttta agtgcaaaat gttcgtttat
    63541 tttttgaggc actacccgtt gttgaacctc gggtagtgcc tcttttttat tgaaaggaat
    63601 caataagatt tgattccttt atttatcaat aggttataat tcatattttt ttattttttc
    63661 ttttacgata cttagtccag aagctaaaat ttcaaggtta ttagaggata gggaaattcg
    63721 aacgaatgca tctggttgcg tacgtgtagt aaagcgtctg gaatgataaa gtcggatacc
    63781 gttagcaaga aagtcattct caattatctt agggtcaatc tcaggatcaa ctggaagaat
    63841 tcgatagtaa gggacacgat tcgttggttt ttctactttg aaaagagaat caaaaagctg
    63901 attggctctt tttgtaagac ttaatttagt ttctaagatc ctatttgctt catttgaatg
    63961 gaggatttga gtgatgactt ccacatctaa tccagaagtt ttcacattta tattaaaaat
    64021 tgcttgttct atcttgcttt ggacatgtac tggaactaca agataagcaa ctcgcaatcc
    64081 agaacaaata aattttgtca ttccagctaa gtagattgtt tggttaggta ataaattttg
    64141 gaaaggagga atgatcgtat gttgatagta agttgttaaa aatgaatgga tatcatcttc
    64201 aataacccat agaccttctt tttcaatcac ttcggctaat tgctctcgtc ttttgtgaga
    64261 gatttgaaac cctataggat tattacaaga aggcataaga aagacaccat gaatcttctt
    64321 ttttctacat tcctgaatca atatatcagg tcgcattcct tgctcgtcaa aatcaattgg
    64381 aacgatctct aaatgatata actgagccaa ttcaatgaaa ttagcgtatg tatatcggtc
    64441 tgttgctatt ctatcacctg gagagaaaag tgcagaaagt gtgatagcca atccattttg
    64501 aacaccggaa acaatagata gagattctaa ggacggaatg actccttgag tttttaacca
    64561 atttgctgca gtagtcaatt gaaaatttgt gcctttggga tcttggtaat taagcaaacc
    64621 aacaagattt gggttattac tagttgattg aataaacggg acaacgaatt tattacattc
    64681 ttcaaaagaa ctaaccagac caaaatcaat gatatgttcg gttacttcat tggtagagat
    64741 ggtgatggac tcaaagacat tgggagaaat aaatgtgcca cttccaatat tcgtatacag
    64801 caaacctttc tcaattccat atttataagc cttgccaacg gtagtaaaat ttaaatctaa
    64861 ataatcagct agctctcttt gggagggtaa ctgagtgttt ttctgtaatt tattctttaa
    64921 aatatcttgt tctaattgtt gaatcaacga tcgataataa ggacgcttca atttttgttt
    64981 gtctggtcgc caagttaacg ggtaattgtc ataagaatta ataggcattt tctccactcc
    65041 ttaaaattgt ataccataca attttaccat tgattgtatt gattttagaa gttaatcttt
    65101 tgtgaggagg gaattgtcta tgaaaattat taattttgag gaagtagtag cttcttttag
    65161 ttttgaagaa gcgattcaag taatgaaaaa atgttttttt gattatgaaa gtggtaaaat
    65221 cagccaaaat cctcgattgg tggaaatttt acctgacgga aaaggcaaaa atatgtttgc
    65281 gatgatgcct gcttatttgg gggagaacag atattttggc acaaaagtca ttaccgcttt
    65341 tccggataat catcagtatc atttaccttc tcatttagga gagattttct tgtttgatgc
    65401 tcaaaacgga ctacccaaag cattgataaa cgcaaatgca gtcacctgga ttcgaacagc
    65461 tgctgtatct gcgctggcaa cagattactt agcaaatccg catgcagaaa cacttgcttt
    65521 aattggagct ggtcaacaag cattgtcgca tttgactgcg atttctacca ttcgtcccat
    65581 cactacagtg tttgtttacg atattttaga agaaagacga aatactttta tcgctaaagc
    65641 taaaaaacag tatcctaaaa taacgtttat aaatgcaata tcagtagaag atgcagtacg
    65701 tccagcggaa atcatttgta cgttgacctc tagtaaagag gcatttcttc aaaaagagtg
    65761 ggtaagggaa gacgcacata tcaatgctgt gggcacattt actcctgata caagagagat
    65821 aagtacatcg ttgatggaaa cttctcaaat ctatgtagat gattactcag ctgccttaac
    65881 agaaagcggg gacctgctta tagccatggc agaaggagat ttagaggaat cttgtattgt
    65941 tggtacatta ggcgaattag tttctgagaa aaaaacattt tataaaggag aatcgaggcc
    66001 gacgattttc gacgcagttg gtctagctat tgaagatctt tgttgtgcag aatatattta
    66061 taacaaatta gagaaagggg ctttaaaatg aacttagatt attacgtagt tgatgcattt
    66121 gcagatgagg tgttcaaagg aaatccagca gcggtttatg tgctagaaga atggctgcca
    66181 gaaggtacga tgcaaaaaat tgccattgaa aacaatttat ctgaaacagc atttacagtg
    66241 aagaaaaacc aagaatttga gcttagatgg tttacgccag atcgagaaat cgatctttgt
    66301 ggtcacgcga cattagcaac agcatttgtt ttatttaact attacaaaat acctgatgaa
    66361 acgattaaat tttctactca aagcggtaat ctatttgtga caagaattaa agattattat
    66421 tacatggatt ttcctagtat tatgccgaaa aaagtgccga tattgtctga atatgaagaa
    66481 gcgattgggg ctaagattaa agaagcctac ttagcacgtg atttattttt cgtattagaa
    66541 gatgaaaaaa cggtggctaa attgcagcca gattttacag ctatcaaaaa ttttgaatta
    66601 ggaataggag tcatcgtgac agcagaaagt gtgcaaaaag actttgtttc tagaaccttc
    66661 tttccaaaat taacaatcaa tgaagatcca gtatgtggat cagcacattc taatcttatt
    66721 ccttattggg cagaaagaat gaataaaaca aaattgtcag cttatcaaat gtcatcaagg
    66781 ggcggggatc tagaatgtga attaaaagaa gatcgtgtga taataggagg aaaagcagtt
    66841 ttgttttcta aaggtacctc gtttatttga tttctttcaa atgaattaaa aataaattgg
    66901 tggagagtgg caaccttata gctacaatgg ttgttaaaaa aagacgcaaa ctttttcaat
    66961 ttaatgaaaa gtttgcgtct cattttaatt attggtgttt gttttttatt caatcaagag
    67021 taaaatgccc catactaact atcctcggat gacttcgatt ttataaccat cagggtcaat
    67081 gacaaagtag taagaaggcg cagtgccagg taatcctttt aagtcagtaa tctcaagacc
    67141 agcagcttta tgttgctcgt gtaatttttc tagatcatca gctgctatag cgatatgacc
    67201 atagccgtct cctagatcat acgcaccatg atcatagttg taagtcaact ctaattcata
    67261 gtcatcccca ggaagtgtca aataaacaag tgtaaatttg ttttctggaa aatcacggcg
    67321 gcggctttct tcaaaaccaa atgccttttg ataaaattcg attgatgctt caagatcttt
    67381 cactcgaaca catgtgtgag ccattttcat agattcatct ctccttttcc ttttactcaa
    67441 tcataacaaa aagaagtaag gaaatagttg atttgtgctt gttcatgaga aatttctcat
    67501 gtaaaatgga tagaaaataa aactgttaag gtggaaacta ttcatggata aaccaatttt
    67561 tggtgaaaaa aagcaatttg ctgattatca aacccgttat gcagcatata ttgtcataga
    67621 aagagatcag cagattgcag taatcgaggc tccaaatggt gctttttttc ttcctggcgg
    67681 ggaaattgaa ggagaagaga cgaaagaaga agcgattgtc cgtgagctga tagaagaaat
    67741 gggaattgtg gcagaaattt cttcttatct gggagaagct gatgagtatt tttactcgaa
    67801 ttatcgtgca acttattatt ataatccagg atatttcttt gttgctgatt cttggaagcg
    67861 tataggagaa ccaactgaaa agaccaataa aatttggtgg gtgagccctc aagaagcgct
    67921 tgtgaaattg aaacgtggta gccaccgatg ggcagtcgag agatggatcg aaaaaaataa
    67981 aaactgaaag aaagcgagta gatcagcttt ctctcagttt ttttatttat tacagtacat
    68041 attttttgat tgcttcagct acaccgtgct cgtcgtttga tgaagtgatg acatttgctg
    68101 cttctttgac aagagaaacg gcatttccca ttgctactcc tagaccagca tattcaatca
    68161 tggataagtc gttctcgtta tctccgatag ccatgatttc atcttgatca ataccaaggt
    68221 gctgtgcgag attcgcaact gccgcacctt tacttgcttc tttatttagt acttcgaagt
    68281 aatagggcgc acttttgacc gttgtgtatt tatcacggaa agtttgcggt aaacgcgcaa
    68341 ttgctgcatc taaaatttct ggttcgtcga tcatcatcat tttgacgatg ttcatgtctg
    68401 gtgtcatttc ttcaggtgtt cgatatttta gcggcatctt gactaaactt gcttcgtgga
    68461 tcgtataagc gctgatatct cgattaggcg tgtagatcgc tttgtcatcg atcgaatgga
    68521 gatgactgcc gacacggcgt gccatcactt cgatctctag gaaatcttca tgtgtcagtc
    68581 cgtgacgggc gatgatttta cctgatttcg tttgttgtac gagtgccccg ttataagtaa
    68641 taacgtaatc attatcttga aaaaggttca actcgtttaa ctgttcctgg acacctggta
    68701 agggacggcc agtgcataaa acgatcgaaa caccttttgc aacagcagcg gcaatcgctt
    68761 cttttacttc ttttgttact tctcttttct catttaataa agtaccatcg atgtcgatag
    68821 cgactagttt gatagacata atttcctcca atatatttta atttatttca atcaatgcgc
    68881 cattacggat atggctggca aattcttgat aagtttcgtc aaataaatca taatgatcac
    68941 gcaaggacgg atcaaccatt tcttttggaa agaaaaatcg ctcatctcct tgttcttgtc
    69001 ccgctaatgc agcaacaagc tgactaacct gtgataattc tatcaaagtt ccgtcatttc
    69061 gcaaaatctc gatttgtgtg cgatgtctgc cttgttctgg gcgataaaaa tcataaggta
    69121 gatcgtagct agaattcaca gcagtgtaat aaacaggatt atatccgacc ttttctacta
    69181 acaaagtgag ttcttggact aattttgaat cccggttgcc agaaaaagta gcagatttca
    69241 gtggtttacg attcaaaaaa cgtttagcta aatcatttaa aataggatca gcagattcag
    69301 accattgagt aaagtaagtg cccaagaccc catcatcaag tttgaggtat tcttctaaag
    69361 taaagcttcc gtcaagaaat ggcagaagta attgagaatg gagttcaaaa ctgctggcat
    69421 cttcttgata caactcgtgt gcgcgatgaa gcagatgctc caaaattact tccataccac
    69481 gggaaacagg atggaagtac acttgcacat acatttgata tcggctaacg atataatctt
    69541 ccactgcatg catgccactc atggaaaaag cgatcccgcc ttcataagga cgaatcacac
    69601 gtaatattcg ggtaagatca aatgttccat attctgttcc agtgaaataa gcatcccgaa
    69661 gcaaatagtc cattcgatct gcatcgatct gactagaaat catttggaca acttgtgggt
    69721 tggggtaagt tttttggatc acactagcta ccttttcagg aaaaccttct tctactcggt
    69781 taagaatctg atacacttct gtttctggag aagtgatgat ctctacagtg atggcttcat
    69841 gattcgtatg gaagatatgt tcaaatgtat gagagtaagg accatgaccg acatcatgca
    69901 acagtgccgc acataaagtc accagccgtt cctgatcatc ccagcctccg ttgcctattt
    69961 tttcttttga aaaatttcga gaaaagatat cgcagattcg tcttgaaatc tcataaacgc
    70021 ctaatgaatg agtaaaacgg ctatgttctg ctccgtgaaa agtgaaagaa gaagtgccta
    70081 attgtttaat gcgtcgtaga cgttggactt ctttcgaatt gatcaaatct aagattactt
    70141 gatgctggac atggatatag ttatggactg gatcacgaaa aactttttcc ataggcagca
    70201 tttgatattt ataaggggtt gtcataatgt agctccttta tgttattacg ttgttccatt
    70261 ctctggattt ggttgttcca ttgctccagc tggatttctt tttttagcca gtcggtagaa
    70321 tcctgtaaat cacttgcaga atacagcttt tcaaaagctt ttgcaaatgc ttctttgact
    70381 tcttttacaa aaaggggttg gtcaagcagt tcttcaagtg ttgccattgt agcaggatcc
    70441 actgcaggat agccatttgt accaaagtct tcttttaaag cagattgata aaatcgcttc
    70501 atgacttgcc cgcgtttttc ctgattaccg ttgacactta agtacatcat aacagaaatc
    70561 ccatttttga tccgacgctg agcgataccg gcaaattttt tgccatggat gcttaaatca
    70621 taagttccag gacaataaga atgggtgatt tcgaatgctt gtatcggtgc ctttggaaaa
    70681 gcgtatttag tcaataggag catttgctcg taagcgtcat ctatggttat tttttttgtg
    70741 tcactttggg ggaaaatcaa agaaatattc aaaattcctt catctgatac gatccctaat
    70801 ccacctgaat ttctgatcac tggagcgtaa cctgcatctt tcagtgtttg gatcccgtca
    70861 cttagaaaag gagttcgaac atccttcata cctaagatga ctgtgcagtc atattgccag
    70921 aaatgtaaaa agatttgctg gtgttcttta gaatattccg ttaatatatc tgttaatgca
    70981 aaaggggaaa agtcgttttt ttgataaatg tgctgatcta gtcgaatcgc agggttcatt
    71041 caaccggctc ctttgtataa attcatattt tctacaatat tatactctaa aatcaaagag
    71101 ttgttttgga aaaaaggttt tctgtaaaaa tggacaagtt ggttgacaaa acaaaagaaa
    71161 ctaggaacaa tgtagctatg agaagtaaag gagaatgaat aatggaatta tcacaaggaa
    71221 caccgatttc aatcaaattg cgtacaaaag taaaacagca aggggaagtg caagattttt
    71281 attttgattt gaaagggcaa atggtgacaa ttggcgatac gctgtacata cgttataaag
    71341 agattcagga ggagacgggc gatgagattc ctgtaacgat caaattagtt ccagatggtc
    71401 atgtacagct gatccgtgca ggagaaatgc ggatgcgttt gaaatttggc tataaagaac
    71461 ggttagaaac atcctatcga acaccatacg gtatgatcca gattgctact tttacgaaag
    71521 aactgcatgt tagtttgaaa gaccgtccca cagcagggaa ggttcgaatc gattatgatc
    71581 tgttcatggg accggaaaaa atcggcgaat attatttgac cttggatttt acagcgtaaa
    71641 tataaaagaa aagtttgatt ttttgtgtct gattttgtat gataaggagt agactgtgaa
    71701 aggacgtgtc ctgattggaa attagtgtat ttgaaggtgc aaacaaaagc gaattatcaa
    71761 tgatcgaagt agcacacgcg atcttagaac aacgtggcga tgtcatggat ttttctgatt
    71821 tagctaacca gatccaaaac tatcttggga aatcagacag tgaaattcgt gattcactcg
    71881 cacaatttta tactgacttg aatattgacg gcagctttat ctcattaggg gataatcgtt
    71941 ggggcttgcg ttcatggtat gctatcgatt cgatcgatga agaagtaaac catggaatgg
    72001 acgaagagga tgaagatact ccacgtcgcc gtaaacgcaa gaaagtcaac gcctttatca
    72061 atgatgatga agacgcgatc gattacaatg acgatgaccc agaagataca gatctgacag
    72121 aagaagatga cgatgatctg tttgatgacg atgatgatga agatgaagaa atcgctgctt
    72181 ataattctga cttgcaagaa atcggagcag atgacccgac agatgatgaa gaagacttgc
    72241 caggaaatat cgaagaagat ttaagtatca tagacgaaga cgatgatgat gaagattatg
    72301 aagaggaaga attttctgac tctgaaaaag aataaacatt attttcttac gagaggttgt
    72361 gacagagacg atcagcctat cagaataagg aggtgtgaca cgatttttgt cacaccttct
    72421 tttttgtata taaaaatccc aagtaaatac ataaaaaata aaaaaattga aacttttagc
    72481 gcaccttgta cgtcatatta agtgaggcag aaaggagtgg agaatgattt ttactggata
    72541 taagaaaaaa cggcagatcg aaaaataccg tcacctgctg aatggactca tcgaaactga
    72601 ttatcaaaaa atgttccgta tcacgatgaa ttacgtacat gataaagaag aggccttaga
    72661 tgtgatgcaa gatagttttc ataaagccct gaactatttt gaaaaagaaa aagagatcga
    72721 gcattttagt gcatggtttt atcggatatt gatccgcacg gcattagata gttggcgcag
    72781 acaaaaaagg acgcctgtgg acttatttga aattcaagat atcccccaac agttaccttt
    72841 agaaacttcc gttgcagagc tccatatgat tttagataaa atagacagtc cggaaaaaga
    72901 gattctgatc ctctactttt ttgaaggatt tcgtttgaaa gaaattgcga ctatcttgga
    72961 tatgaatgaa aacacagtta aaacgaaaat gtaccgctca ttgaatgctt tacgtcaaat
    73021 cctagaatga aggagagaag agtgccatgt attcccaaaa agaaaattta aaaaaactaa
    73081 agaaaactta taaagaacaa gcaattccca aagggcttaa agaggagatc cgccagcgtt
    73141 ttattatcga agagcaatca tttataaaag caaagcggag aaagagacgt ctccaagggt
    73201 tcggagtatc tttggctatc gtaatgatta caacaagtag ccttcttttt aatagtcaag
    73261 ttcgaagctt tgcagaagat ttacccatct taggatcagt cattgaattg atcttaggcg
    73321 aacgctttac tgatcgatct gaaaaaatcg atattcaggt accgaagatc aatacacaaa
    73381 atgagaaaga aaataaaaca attcatggat taaatcaaaa atactttcgc gaaggacaag
    73441 cagattttga aaaagctgaa aaagaatatg gaaattttga aacggatcat taccaagttt
    73501 tgggagatta tcagaaaatc gtagacgata accggtttct cgtgattgaa aggcagatca
    73561 cccaaacagc agcagatagc catgttgaaa aaagatacga cacaatcgac aagaaaaaca
    73621 gtgtccagct ctcacttcca cttttattca aagatgacac ctatctatct gttttgacaa
    73681 aagaagtcaa acggcagatg gccgaacaag tcaaggaaga tccatctaag tactattgga
    73741 ctgagcaaga tattcaagaa gggacgatag aaaaaccaac acttgttacg cctacacgaa
    73801 gtttttattt gaacaaagag catcaattag ttttgacttt ttctcaatac gaaattgctc
    73861 ctggttatat gggtacacca gaatttgtca ttcctaagtc cgtgacaaaa acgattttgg
    73921 catcggaaga ttatttggat cattagacga gaagtagttg cgactagcag tttcaatttt
    73981 caagtaagta caagtaaata ataagaagag aaaaagagaa agaagagata tagtgaaaag
    74041 aaattattta gttatcagta ttattgcttt atccgtgctt ttgtttttcg aaattacttc
    74101 ggtggcctcc ttgatcaccg aagtaaaagg acaaacacac tcttcggagc attcgtcaca
    74161 atctttcaaa caatcagaac aatcaaccat taaaagccaa tcaactgaaa cagctaacgt
    74221 caaagaagcc attcaagaat cagcggaaaa ggtacaaagt cagtcatcac aatcaacaca
    74281 agacagtaca aaaacgagcc gagatccggc tgtcaaaagc gtcgcaatca gctttgatga
    74341 tggaccagga gcgaccacaa caccgcagct tctgcgaatc ttaaaagaaa aaaacgtgca
    74401 tgccactttt ttcgtcttag gagaaaatac ggcgcagcat cctgaaatcg tgaagcagac
    74461 agctgaagca gggcacgaga ttggcaatca cacatacgat catcagaatc tggccactct
    74521 ttctgcccag tcgatgacgg aggaagtgac gaaagcagac actgagatca aaaaagtcac
    74581 tggcaaaacc cccacttttg ttcgtccgcc ctatggttct gtcacgaatg taggagcaac
    74641 gatcatccag cgaccgatca tcgaatggtc tgttgattca gaagattgga agacaagaaa
    74701 tcctgatttg attttgcaga agatccaagc aacagtatac gatggggcaa ttattttatt
    74761 ccatgatatc tatcctgaaa cgattcgtgc cgttcctcaa gtgatcgatt atctgaaaga
    74821 gcaagggtat cgcatcacga cagttgggga tttattaggg catccaacgg ctgtcgagaa
    74881 ctattatggc agaaacgatc atcggccagt tcagtaagtg agacaagtat ctttaataaa
    74941 taacacattt taataagtag ttgaacacat aactacctta cttgcaaagg cttaacaata
    75001 tctatacagg tacaacaaaa aactgtccac caattttctt tggcggacag ttttcactta
    75061 aaaataaaga agcaactatt cgtaaattat gctaaaatag cagtagaacg cgtcctgagg
    75121 gaatcgaacc cccgtctcaa gaaccggaat cttacgtgat atccactaca ctaaggacgc
    75181 aagcactggg ataattgtaa ctaaacagat aaaaaattac aaggggattg gacaaaaaaa
    75241 tgaaatttga gcaaaatttt tcacaaaaac agcagcagac acagaaacta gccatgacgc
    75301 aacagttgca acagtccatc caagttttac aatataacag tgaggagctg ttggcattcg
    75361 ttgagaatca agcaatggaa aatccattgg tggaagttgt tgagccggaa tggcagccag
    75421 attacataaa agcttcttca tcttcttatg aaggagaaga gacaaattac ctaaatcaga
    75481 ttcctgatac aaaagggtct ttatttgatt cattgatcga acaagtccat ttgaattacc
    75541 gagatacttt tttgaggaaa atcgttcttt atcttgtaga atacatcgat ttgaacggat
    75601 ttttgacgat tagtttagaa gaagccggaa aagagattgg agctacgcct atccaaatgc
    75661 tcgatgcgtt gactctgatt cagcaattag aaccagctgg agtaggtgca cgtagtttgc
    75721 aagaatgtct catgcttcaa acagaacgag atgattatgc gccggaatta gcttatatcg
    75781 tgttggaaga atgttttgag gaactggtgg agcgaaaatg gaaagaaatc gcacaacgtt
    75841 tttctgttga tctacatgct gttcagcaga tattcgacta tttacaaacg ttgagtccaa
    75901 gccctggccg gattttcgac cgtagcagcg aattgtacat tattcctgat gtccgggtat
    75961 tagtggacga cgataaaaat gtacaagtta tctcaaaccg aaaaaatcag ccaaatatac
    76021 gctttcaaga gagttacttc aaacaaatgt cacaacaagc agataaagaa actgaaaatt
    76081 atttaaaaga acggaaacag gaattcgaat ggttgaagaa aacgatccta caacgtgggg
    76141 atacaatttt gcgggttgct caagtcattg tttcccggca aaaagctttc tttatcgaca
    76201 aggaacgtcc aatcaagcct cttacgttaa aggaagttgc aactgagata gatgtgcatg
    76261 aatccacggt cagtcgtgca gtcaacggga aatatttgga aacagacttc ggtgtatttg
    76321 aattaaaaaa atttttcact acacgaattc ctacgaatag tacagaacag acagaagacc
    76381 tttcagcaga tacagcgaag aaaaagctgc aggaattagt cgatcaagaa gacaaaaaca
    76441 agccgttgtc tgaccagaaa ttggttgaat tgctgaaaaa agatgagata gcgatctcaa
    76501 gacgaacagt agcaaaatat cgtgatttat taggaatccc gagttcatca aaaagaaaac
    76561 gttatgataa ctaatcagtc aaaagactag aaaaagtatt tcactttttc tagtcttttt
    76621 agttgaaaaa cgagagcaaa actgttagac ttacagtcgt gaggttgaga tttcccttta
    76681 ttgatgtgaa attcgaatag ttaatttctt tttttatcgt atatgggtcg ttttaagtct
    76741 acattggacg taaagtgtcc aactaaggag atcgctatgc tggatgaaat aaaaatgatt
    76801 gaatctgttg caccagatat tatcgaagtg ttgcaagaac gctataaaat tttacgaaat
    76861 atctattgga tgcagccgat tggacgtcga agtttatcgg aaagtatggg gatcacagaa
    76921 cgagtattgc gaacagaaac agatcttttg aaaaatctga atttgatcga cacctcaaaa
    76981 agcggtatga cgctgacaac taagggcgaa gaagtttatc aaagccttga aaacttcatg
    77041 gatcagcttt taggtacgca tcaaaccgaa caacagttgg cagaatattt tggtaaccaa
    77101 cgctgtatcg ttgtttcagg aaacagcgaa gagcagacca aagtggctga tgcattcggc
    77161 gaagcgctga gcgaagccct agaccgacta cttccagaag gcgagaatat catcgctgtg
    77221 atgggaggaa ctactatggc tgtggtagcc gaacaattgt ctaatctgga aaataagaaa
    77281 cggcataacc tgtttgtccc tgcaagaggc gggattggtg aagcgataac tgtacaggca
    77341 aattctgtca gtgcaagaat ggctgctaaa gcaaacggta accaccgtgc actttatgtt
    77401 ccggaacaat taagtctggc aacttacaat tctttactca atgagccatc tatccaagaa
    77461 gtcttaaact tgattagtga agcgaattgt gttattcata gtattgggcg tgcattacac
    77521 atggcagcac gtcgtaagat gactgaaaaa gaattagtca tgctgaaaca agcaaatgct
    77581 gttgcggaat cttttggcta ctttttcaat gaaaaaggtg aagtcgttta taaggtccct
    77641 agaatcggca tagaattgca agatttggaa aaagtcccga tcatcatggc aatcgcgggc
    77701 ggaaaatcaa aagctaaagc aattcgagct tatatgaaaa atgcaccaaa acaaacgtgg
    77761 ctcatcactg atgaagctgc tgcaaatgag attttaaaag gggtaaccct ttaaaataaa
    77821 tatttttttg attttcataa ggaggaaatc ttaaatgaca gttaaagtag gtattaatgg
    77881 ttttggacgt attggacgtc tagcattccg tcgtatccaa gatgtagatg gaatcgaagt
    77941 agtagcaatc aacgacttga cagatgctaa aatgttggca cacttgttaa aatacgacac
    78001 aactcaagga cgtttcaacg gaacagttga agttcatgaa ggttcattca acgtaaacgg
    78061 aaaagaagtt aaagtattag ctaaccgcaa cccagaagaa ttaccatggg gcgaactagg
    78121 cgtagatatc gttctagaat gtactggttt cttcacttct aaatcagcag ctgaaaaaca
    78181 tttaacagct ggtgctaaac gcgttgttat ctctgctcct ggcggaaacg atgttccaac
    78241 aatcgtttat aacacaaacc acgaaacatt aactggtaaa gaaacagtta tctcaggtgc
    78301 ttcatgtact actaactgtt tagctcctat ggctaaaaca ttgaacgaca aatttggtgt
    78361 tgttgaagga ttaatgacaa ctatccacgc ttacacaggt gaccaaatga ctctagacgg
    78421 acctcatcct aaaggtgact tccgccgtgc acgcgctgct gctgcaaaca tcgttcctaa
    78481 ctcaacaggt gctgctaaag ctatcggttt agtaattcct gaattgaacg gtaaattaga
    78541 cggagctgct caacgtgttc ctgtaccaac aggttcatta acagaattag taacagttct
    78601 tgaaaaagaa gtaactgttg acgaaatcaa tgcagcaatg aaagaagctt caaacgaatc
    78661 ttacggatac aacacagacg aaatcgtttc ttcagatatc gttggtatga cttacggttc
    78721 attatttgat gctacacaaa ctaaagtaat gacagttggc gacaaacaat tagttaaaac
    78781 tgttgcttgg tacgacaacg aaatgtcata cactgcacaa ttagtacgta ctttagaata
    78841 cttcgctaac ttataagata gacaaacgta caagtgtaaa ttatagacat gtacatttaa
    78901 aagcggggaa gcaatcgcgc ttcttccgct ttttttgata ataagtttat tcaggaggaa
    78961 ataaaatggc taagaaaaca gtgaaagata tcgatttaaa agacaaaaaa gttcttgtcc
    79021 gtgttgactt caacgttcct ttgaaagacg gcgtgatcac tgacgacact cgtatcaaag
    79081 cagcattgcc aacaatcaac tacgttctag aacaaggcgg aaaagcaatc cttttctctc
    79141 accttggacg tgtaaaaaca gaagaagata aagaaggaaa atctttagct ccagttgcaa
    79201 aacgtctagg cgaattatta ggtaaagaag taacattcgt acctgaaact cgcggagaac
    79261 aattagaaga agcaatccgc aacatgaatg acggcgacgt tgtcgtattc gaaaatacac
    79321 gatttgaaga tattgacggt aaaaaagaaa gcaaaaatga tacagaacta ggtaaatact
    79381 gggcttcatt aggcgatgta tttgttaacg atgctttcgg tacagctcac cgtgcacatg
    79441 cttctaacgt aggtatcgct tcaactggta tcccaacagt tgctggattc ttgatggaaa
    79501 aagaaatcaa gttcatcggt gaagcagtag aagaaccaaa acgaccaatg attgcaatcc
    79561 ttggtggcgc aaaagtttct gataaaatcg gcgtaatcga aaacttgatt cctaaagcag
    79621 ataaaatctt gatcggtggc ggaatgactt acacattcta cgaagcaaaa ggaatcaaga
    79681 tcggtaactc tctagtagaa gctgataaag tggaactagc aaaagaatta atcgaaaaag
    79741 caggcgacaa acttgtcttg ccaatcgaca atgtctgtgc accagaattt tcaaatgatg
    79801 ttgaaacaca agtaatcgaa ggagacattc ctgatggttt gatggcttta gacatcggcc
    79861 cagcatcagt aaaattattt gctgatacat tacaaggtgc aaaaacagtt gtttggaacg
    79921 gacctatggg tgtatttgaa atgagtaact ttgctaaagg tacaatcggt gtttgtgaag
    79981 caatcgctaa cttagaaggc gcaacaacaa tcatcggtgg cggagactca gccgcagcag
    80041 cagaacaatt aggatttgct gacaaattca cacacatctc aacaggtggc ggcgcaagct
    80101 tggaattgct tgaaggtaaa gaattaccag gactagcagc aatcaacgat aaataaacga
    80161 ccaagtttgc tcaattctga cgagtaatcg aacaaaaggt gaagggagtt ttcatgactc
    80221 cctagccatt tttcaataaa ttaaggagtg tgttcctcaa tgcgtaaacc tattatcgca
    80281 ggtaactgga aaatgaacaa aactgcatca gaagcaaaag aatttgctga agcagtaaaa
    80341 aacaaaatcc cttcaaatga caaagttgat tcagtcatcg gatctcctgc attattctta
    80401 caagaattaa tgacatcagc tgaaggtaca gaactaaaaa tctcagcaca aaactgctac
    80461 tgggaaaact caggtgcgtt cactggtgaa acttcaccag cagctcttgc tgatctaggt
    80521 gttcattatg tgatcatcgg acattcagaa cgtcgtgaat atttccatga aacagacgaa
    80581 gacatcaata aaaaagcgaa agctatcttt gcaaatggta tgacaccaat cctatgctgt
    80641 ggcgaatctt tagaaaccta tgaagcaggc aaaactgctg aatggatcga aggacaaatt
    80701 acagctggtt tagctgatct ttcagctgaa caggtgtcaa acatggttat tgcttatgaa
    80761 ccaatctggg caatcggtac tggtaaatca gctgacgcac aaatcgctga cgaaatttgt
    80821 ggagttgtac gtcaaacagt tgaaaaactt tatggaaaag aagtatcaga agcagttcgt
    80881 atccaatacg gcggttctgt aaaaccagaa aatattgctg aatacatggc aaaagaaaat
    80941 gttgacggtg ctttagtcgg tggcgcaagc ttgcaagcag attcattctt gtcattgtta
    81001 gacgctgtaa aataattgtt gaaaatttgc gaaaaatgca agaaatgttt gcactaatga
    81061 gcaaaattaa ctaaaatcgt atatgaggga aatcccttag gaaaaaacaa actcaaagga
    81121 gagacaaaac atgtcaatta ttactgatgt ttacgctcgc gagatcttag actcacgtgg
    81181 taacccaaca atcgaagtag aagtatacac agaaagcgga gcttttggcc gcggaatggt
    81241 tccttcaggt gcttcaactg gtgaatacga agcagttgaa ttacgtgacg gagacaaagc
    81301 tcgttatggc ggtaaaggtg taactaaagc tgtcgacaac gtaaacaaca tcattgctga
    81361 agcaatcatt ggctacgatg ttcgtgacca aatggctatc gacaaagcta tgatcgcttt
    81421 agacggaact cctaacaaag gtaaattagg tgcgaacgct attttaggtg tttctatcgc
    81481 tgttgcacgt gcagctgctg actacctaga agtaccttta taccactacc taggcggatt
    81541 caacacaaaa gtattgccaa caccaatgat gaacatcatc aacggcggat cacatgctga
    81601 taactcaatc gacttccaag aattcatgat catgcctgtt ggcgctccaa cattcaaaga
    81661 agctctacgt atgggtgctg aagtattcca cgcattagct tcaatcttga aagctcgcgg
    81721 attagcaact tctgttggtg acgaaggtgg atttgctcca aaccttggtt caaacgaaga
    81781 aggttttgaa gtaatcatcg aagcaatcga aaaagctggc tatgtacctg gtaaagacgt
    81841 tgttcttgct atggatgctg cttcttcaga attctacgac aaagaaaaag gtgtttacgt
    81901 actagctgat tcaggcgaag gcgaaaaaac aactgacgaa atgatcaaat tctacgaaga
    81961 attagtttct aaatatccaa tcatctcaat cgaagacgga ttagacgaaa acgactggga
    82021 cggattcaag aaattaactg acgtattagg cgacaaagtt caattagttg gtgacgactt
    82081 gttcgtaaca aacactcaaa aattatctga aggtatcgaa aaaggaattg ctaactcaat
    82141 ccttatcaaa gtgaaccaaa tcggtacatt aacagaaact ttcgaagcta tcgaaatggc
    82201 taaagaagct ggctacacag cagttgtatc tcaccgttct ggtgaaacag aagattcaac
    82261 aatctctgat atcgctgttg caacaaacgc tggccaaatc aaaactggtt ctctatcacg
    82321 tactgaccgt attgctaaat acaaccaatt actacgtatc gaagaccaac ttggtgaagt
    82381 tgcagaatac aaaggtttga aatctttcta caacttaaaa aacaaataat tcttttctaa
    82441 tgaagataca tgatcgacta ttgaaagata gtcgagtatg atcacttagt taagtaatcg
    82501 aactctctac ttgctgaaat atcagcaggt agagagtttt tttgcaacta gcttctgtat
    82561 atgatgttta caaagaatgg tacaagaaaa gatcatcctt gaaaaaggaa agctaacaat
    82621 aactagcatc atataagagc ttttaaggat agaacagtag aataggaatc tatgagcaag
    82681 tcctctcgta taaataaaat aggagggaag tagcagcata tttttcataa agtggctctc
    82741 tttcttcatt tttactttgg ttgctcctat ttgaaaattc gagcacactc aaatacaata
    82801 aaaagaaatg gcaagggaga ggattagatg aaaaaagaag aaaaaatcgc gattttacaa
    82861 gagatcatcc gaattaaatc agtgaatgga aacgaaggtg aagtagcagc ctatttgaat
    82921 aagcttttgg caaagcatga cataacagga gagattgttt cttacagaga tggtagagat
    82981 aatttgattg ctcgttacca aaagggccaa tcaggaaaag tgttaggcct atctgggcat
    83041 atggatgtcg tggcggctgg tgatgaatcg tcttggacct atgcaccctt tgcagcagaa
    83101 atccatggga accgtttgta cggacgtggt gctacagata tgaagagtgg cttggcagca
    83161 atggtgattg ccatgatcga actgaaagag tcaggaaaac catttaacgg aacagtcaaa
    83221 ttattagcca ctgtcggaga agaagtgggt gaactaggtg gggaacagct cacgaaagca
    83281 ggctatgtag atgacttaga tgccttgatc atcggagagc caaccaatta cagtttgatg
    83341 tatacgcaca tgggatcaat caactataca gtcacctcgc acggaaaaga agcgcatagc
    83401 tctatgccag accaaggtta taatgcaatc aatcacctaa atgaattcat tacgaaagca
    83461 aatgctgaaa tgaatcacct cgctgaaaca atcgagaatc ctgtcctagg aaaaacaatc
    83521 cataatgtta cattgatcag tggcgggaat caagtcaaca gcatcccaag ccatgcgcag
    83581 ctccaaggga acattcgatc gattccagaa tacccaaatg ataaaatcat tgcattgctg
    83641 caatcaatcg tcaatgaatt gaatcaagag acagactatc atttggaatt gatgatcgat
    83701 tacaacaaaa tacctgtcaa agctgatcca gattctcctt tgattcattg cattcagcaa
    83761 caattcagcc agccattacc tttagttgga gcggcagcaa caacggatgc agccgaattt
    83821 acgaaagcta accattcctt cgactttgtc gtctttgggc ctggtgtcgt tactttgcct
    83881 catcaagtag atgagtatgt tgaaatcgat aattatttgg atatgatcga aaaatatcaa
    83941 ggaattattt tatcctactt agcgtaagat ttttttaata aatgaaaaaa cagagccaga
    84001 ttagcatt
//
LOCUS       DANKMN010000003        83001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000003 DANKMN010000000
VERSION     DANKMN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..563)
                     /locus_tag="PW224_000178"
     CDS             complement(<1..563)
                     /locus_tag="PW224_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL2906898.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="PW224_000179"
     CDS             complement(750..1400)
                     /locus_tag="PW224_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906899.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="PW224_000180"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="PW224_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL2906900.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="PW224_000181"
     CDS             complement(2912..3910)
                     /locus_tag="PW224_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL2906901.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="PW224_000182"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="PW224_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL2906902.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="PW224_000183"
     CDS             complement(4926..5657)
                     /locus_tag="PW224_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL2906903.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="PW224_000184"
     CDS             complement(5661..6926)
                     /locus_tag="PW224_000184"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL2906904.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="PW224_000185"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="PW224_000185"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL2906905.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="PW224_000186"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="PW224_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL2906906.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="PW224_000187"
     CDS             12268..13770
                     /locus_tag="PW224_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2906907.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="PW224_000188"
     CDS             13772..14509
                     /locus_tag="PW224_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2906908.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="PW224_000189"
     CDS             complement(14554..14727)
                     /locus_tag="PW224_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906909.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..15368
                     /locus_tag="PW224_000190"
     CDS             14703..15368
                     /locus_tag="PW224_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2906910.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="PW224_000191"
     CDS             complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="PW224_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL2906911.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="PW224_000192"
     CDS             complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="PW224_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL2906912.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="PW224_000193"
     CDS             complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="PW224_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL2906913.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="PW224_000194"
     CDS             complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="PW224_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL2906914.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20581..21747)
                     /locus_tag="PW224_000195"
     CDS             complement(20581..21747)
                     /locus_tag="PW224_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL2906915.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21763..21990)
                     /locus_tag="PW224_000196"
     CDS             complement(21763..21990)
                     /locus_tag="PW224_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL2906916.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22474..23064
                     /locus_tag="PW224_000197"
     CDS             22474..23064
                     /locus_tag="PW224_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL2906917.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23254..23967
                     /locus_tag="PW224_000198"
     CDS             23254..23967
                     /locus_tag="PW224_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2906918.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24082..25092)
                     /locus_tag="PW224_000199"
     CDS             complement(24082..25092)
                     /locus_tag="PW224_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL2906919.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25129..25611)
                     /locus_tag="PW224_000200"
     CDS             complement(25129..25611)
                     /locus_tag="PW224_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL2906920.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25761..26129
                     /locus_tag="PW224_000201"
     CDS             25761..26129
                     /locus_tag="PW224_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL2906921.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26594..27046
                     /locus_tag="PW224_000202"
     CDS             26594..27046
                     /locus_tag="PW224_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2906922.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27170..28021)
                     /locus_tag="PW224_000203"
     CDS             complement(27170..28021)
                     /locus_tag="PW224_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL2906923.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28034..28819)
                     /locus_tag="PW224_000204"
     CDS             complement(28034..28819)
                     /locus_tag="PW224_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2906924.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="PW224_000205"
     CDS             complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="PW224_000205"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL2906925.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30116..31039)
                     /gene="truB"
                     /locus_tag="PW224_000206"
     CDS             complement(30116..31039)
                     /gene="truB"
                     /locus_tag="PW224_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL2906926.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31407..33769)
                     /locus_tag="PW224_000207"
                     /pseudo
     CDS             complement(31407..33769)
                     /locus_tag="PW224_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34217..34390
                     /locus_tag="PW224_000208"
     CDS             34217..34390
                     /locus_tag="PW224_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2906927.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="PW224_000209"
     CDS             complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="PW224_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL2906928.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34921..37227)
                     /gene="infB"
                     /locus_tag="PW224_000210"
     CDS             complement(34921..37227)
                     /gene="infB"
                     /locus_tag="PW224_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL2906929.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37240..37551)
                     /locus_tag="PW224_000211"
     CDS             complement(37240..37551)
                     /locus_tag="PW224_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL2906930.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37548..37841)
                     /locus_tag="PW224_000212"
     CDS             complement(37548..37841)
                     /locus_tag="PW224_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL2906931.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="PW224_000213"
     CDS             complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="PW224_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL2906932.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="PW224_000214"
     CDS             complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="PW224_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL2906933.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39679..44037)
                     /locus_tag="PW224_000215"
     CDS             complement(39679..44037)
                     /locus_tag="PW224_000215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2906934.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44249..45958)
                     /locus_tag="PW224_000216"
     CDS             complement(44249..45958)
                     /locus_tag="PW224_000216"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL2906935.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="PW224_000217"
     CDS             complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="PW224_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL2906936.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47455..48255)
                     /locus_tag="PW224_000218"
     CDS             complement(47455..48255)
                     /locus_tag="PW224_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL2906937.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48252..49064)
                     /locus_tag="PW224_000219"
     CDS             complement(48252..49064)
                     /locus_tag="PW224_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL2906938.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49259..49816)
                     /gene="frr"
                     /locus_tag="PW224_000220"
     CDS             complement(49259..49816)
                     /gene="frr"
                     /locus_tag="PW224_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL2906939.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49819..50541)
                     /locus_tag="PW224_000221"
     CDS             complement(49819..50541)
                     /locus_tag="PW224_000221"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL2906940.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50677..51558)
                     /locus_tag="PW224_000222"
     CDS             complement(50677..51558)
                     /locus_tag="PW224_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL2906941.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="PW224_000223"
     CDS             complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="PW224_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL2906942.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52798..53277
                     /locus_tag="PW224_000224"
     CDS             52798..53277
                     /locus_tag="PW224_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL2906943.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53494..54585
                     /gene="phnW"
                     /locus_tag="PW224_000225"
     CDS             53494..54585
                     /gene="phnW"
                     /locus_tag="PW224_000225"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDL2906944.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54578..54706
                     /locus_tag="PW224_000226"
     CDS             54578..54706
                     /locus_tag="PW224_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2906945.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54710..55255
                     /locus_tag="PW224_000227"
     CDS             54710..55255
                     /locus_tag="PW224_000227"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2906946.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55709..57400)
                     /locus_tag="PW224_000228"
     CDS             complement(55709..57400)
                     /locus_tag="PW224_000228"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL2906947.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="PW224_000229"
     CDS             complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="PW224_000229"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL2906948.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58882..59901)
                     /gene="argF"
                     /locus_tag="PW224_000230"
     CDS             complement(58882..59901)
                     /gene="argF"
                     /locus_tag="PW224_000230"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL2906949.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="PW224_000231"
     CDS             complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="PW224_000231"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL2906950.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            61682..62383
                     /locus_tag="PW224_000232"
     CDS             61682..62383
                     /locus_tag="PW224_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2906951.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(62992..64008)
                     /gene="tsaD"
                     /locus_tag="PW224_000233"
     CDS             complement(62992..64008)
                     /gene="tsaD"
                     /locus_tag="PW224_000233"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL2906952.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(64005..64469)
                     /gene="rimI"
                     /locus_tag="PW224_000234"
     CDS             complement(64005..64469)
                     /gene="rimI"
                     /locus_tag="PW224_000234"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2906953.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(64476..65018)
                     /gene="rimI"
                     /locus_tag="PW224_000235"
     CDS             complement(64476..65018)
                     /gene="rimI"
                     /locus_tag="PW224_000235"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2906954.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(65002..65724)
                     /gene="tsaB"
                     /locus_tag="PW224_000236"
     CDS             complement(65002..65724)
                     /gene="tsaB"
                     /locus_tag="PW224_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL2906955.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(65915..66895)
                     /locus_tag="PW224_000237"
     CDS             complement(65915..66895)
                     /locus_tag="PW224_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2906956.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(66919..68403)
                     /gene="galT"
                     /locus_tag="PW224_000238"
     CDS             complement(66919..68403)
                     /gene="galT"
                     /locus_tag="PW224_000238"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL2906957.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(68415..69404)
                     /gene="galE"
                     /locus_tag="PW224_000239"
     CDS             complement(68415..69404)
                     /gene="galE"
                     /locus_tag="PW224_000239"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL2906958.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            69652..69819
                     /locus_tag="PW224_000240"
     CDS             69652..69819
                     /locus_tag="PW224_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL2906959.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(69881..71692)
                     /locus_tag="PW224_000241"
     CDS             complement(69881..71692)
                     /locus_tag="PW224_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL2906960.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(71689..72054)
                     /locus_tag="PW224_000242"
     CDS             complement(71689..72054)
                     /locus_tag="PW224_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL2906961.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(72217..72612)
                     /locus_tag="PW224_000243"
     CDS             complement(72217..72612)
                     /locus_tag="PW224_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2906962.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(72630..73592)
                     /locus_tag="PW224_000244"
     CDS             complement(72630..73592)
                     /locus_tag="PW224_000244"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL2906963.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(73592..73804)
                     /locus_tag="PW224_000245"
     CDS             complement(73592..73804)
                     /locus_tag="PW224_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL2906964.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(73825..74523)
                     /locus_tag="PW224_000246"
     CDS             complement(73825..74523)
                     /locus_tag="PW224_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2906965.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(74543..75085)
                     /locus_tag="PW224_000247"
     CDS             complement(74543..75085)
                     /locus_tag="PW224_000247"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL2906966.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(75217..76221)
                     /locus_tag="PW224_000248"
     CDS             complement(75217..76221)
                     /locus_tag="PW224_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2906967.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(76218..77207)
                     /locus_tag="PW224_000249"
     CDS             complement(76218..77207)
                     /locus_tag="PW224_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2906968.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(77204..78010)
                     /locus_tag="PW224_000250"
     CDS             complement(77204..78010)
                     /locus_tag="PW224_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2906969.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            78176..79132
                     /locus_tag="PW224_000251"
     CDS             78176..79132
                     /locus_tag="PW224_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2906970.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(79209..79727)
                     /locus_tag="PW224_000252"
     CDS             complement(79209..79727)
                     /locus_tag="PW224_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2906971.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(79815..79964)
                     /gene="rpmG"
                     /locus_tag="PW224_000253"
     CDS             complement(79815..79964)
                     /gene="rpmG"
                     /locus_tag="PW224_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2906972.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            80192..80638
                     /locus_tag="PW224_000254"
     CDS             80192..80638
                     /locus_tag="PW224_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2906973.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(80833..82728)
                     /gene="asnB"
                     /locus_tag="PW224_000255"
     CDS             complement(80833..82728)
                     /gene="asnB"
                     /locus_tag="PW224_000255"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL2906974.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        23581 a        17672 c        14336 g        27412 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaccaggcga acatatgatt gttcgtctgg tttttaatta
    15421 ttaatgactt agcgttttta ctagaaaaac tacttccgct ttttcccgcc aaaagcatct
    15481 ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc gccagcttca
    15541 gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt gatcagtttt
    15601 actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc ttttccacgt
    15661 aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac atctccatgg
    15721 acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa aggcaattca
    15781 tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg gaagaccaca
    15841 tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc taaacgcatt
    15901 tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc tttcttctca
    15961 tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt cccatgacaa
    16021 acatcacaag tttgacggct catcactcgt ccaagtggtg tttggcgttc tacattaatc
    16081 gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt tgcaccgttc
    16141 ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc tacaccaaaa
    16201 atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg acgaggcgca
    16261 tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc ttcaaaacca
    16321 ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc tccaccagca
    16381 ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat atgcagcgcg tttttgcggg
    16441 tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc atcaggttct
    16501 ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt tatttcatct
    16561 tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt tgccataggc
    16621 tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat attttgtttg
    16681 aaacaaaaac cgatacttca aactattttt caaaagaaaa agccaaagta gcaactttgg
    16741 ccttctctcg aaattcagtg aactgattat ttatcgtcgc catcgacttc ttcaaaatca
    16801 gcatcaacga catcatctgc tccgccttga gctgcttctg ggttttcttg agcttgttgt
    16861 tgtgccgctt gttcgtatag tttaacagtc aagttttgaa cgatttcgtt caatgcatcg
    16921 cgtttagttt tcatttcttc gatgttgttt gcttcaacag cggctttcaa ttcatcgcga
    16981 gctgtttctg cttttttcac ttcttcttcg tccactttgc cttctaattc tttcaatgtt
    17041 ttgtccacag agaatagcaa tgcatctaca tcattacgta ggtcaacttc ctctttacga
    17101 gctttatctg cttctgcatt tgcttctgca tcttttacca tacgttcgat ttcttcgtct
    17161 gttagacctg aagaagattt gatcgtgatt ttttgttctt tttgtgtacc tagatctttc
    17221 gcagatacat ttacaatccc gtttttatca atatcgaatg ttacttcgat ttgtggaata
    17281 ccacgaggag ctgctggaat atctgtcaat tggaatcttc ctagagtttt gttgtcagca
    17341 gccattgggc gttcaccttg taatacatgg atgtctactg ctggttgatt atctgccgca
    17401 gttgagaata cttgtgattt acttgttgga atcgttgtat tgcggtcaat caatttagtg
    17461 aacactccac ccattgtttc gatacctaat gacaatggtg taacgtcaag taaaacaaca
    17521 tctttcacat caccagtgat cacgccacct tggatagcag cacccatagc aactacttca
    17581 tcagggttaa ctgatttgtt tggttctttt cctgtttctt tacgtactgc ttctacaact
    17641 gcagggatac gagtagatcc accgactaaa ataacttcgt cgatttctga ttgtgataaa
    17701 cctgcatctt ttagcgcttg acgtactggt acttttgtac gttctactaa gtcagctgtt
    17761 aattcatcaa attttgcacg tgtcaatgtc atttccaaat gcaatggtcc agattctcct
    17821 gctgtgataa atggcaagct gatttgtgtg cttgatacgc cagataaatc ttttttcgct
    17881 ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt cttgagacaa gtcaatgctg
    17941 ttttcttttt tgaattctgc taccatgtag tcgatgattt tgttatcaaa gtcgtcccca
    18001 cctagatggt tgtcgccagc tgttgacaat acatcaaaga caccgtctcc tagttcaagg
    18061 atagatacgt caaaagtacc gccaccaagg tcgaatacta agattttttc gtcgcgatct
    18121 gttttatcta aaccataggc aagagcagct gcagttggtt cattgacgat acgttctact
    18181 tctagaccag cgatcttacc agcatctttt gttgcttgac gttgtgcatc attaaagtaa
    18241 gctggcacag tgataactgc tttttcgact ttttcaccta aatagtcttc tgcgaatcct
    18301 ttgatatatt gaaggatcat tgctgagatt tcttgtggtg tatatgattt accttctaca
    18361 tctactttgt aacctgcttc accgatatgg cgtttgattg atgaaatcgt atttgggttt
    18421 gtcacagctt gacgttttgc aacttcccct acttggattt ctccattttt gaatgataca
    18481 acagaaggtg ttgtacggtt tccttctggg ttagcgatga tttttgcttc gtttccttcc
    18541 aatactgcta ctgcagagtt agtcgtacct aagtcaatac cgataatttt actcataatt
    18601 aacgtctcct tctttaattg aaagtgtaag ttttatatcc gcacaattat tgtgcaacaa
    18661 tgaccatggt tggtcttaac acgcgatcat gtaatttgta gccttcttgt agtacttcta
    18721 caatcgtatc ggctggtgta tcttcagtcg ctggaacagt ttgtactgct tgatgaagat
    18781 taggatcaaa ggcttctcct ttagcaggga ttttttcaat tccttcttct tccagtgcgt
    18841 tacgcaaact ttccaagacc atttccacgc cttttttcaa acctgcccct tgatcatctg
    18901 acacttctgt ggctaatgca cgttccaggt tgtcaattga tggaagtaat tttttggcta
    18961 aatcttgtga acggtatttt tgtaattgct cacgctcatt ttttcctcga tttgccatat
    19021 ttgcgatttc agcacgagcc cgcaagtatt tatcttccat ttcttctaac tcagctttta
    19081 atttcgctgt ttcgaattct tccgcttcag cttctgaaat acccgaatct tctgcagctt
    19141 cgacatcgat ttccggttct ggctgagcat ctgtcatttc tttatctagt tcttcttgat
    19201 tttctttcac gatacagtct tcctttcatt attcagtcag ctgtttgatt gctcttacta
    19261 agaaagagag aacgatcagc tgcaaatagc ttcgttattc acattacgag tttgacaagt
    19321 caagcgaacg gtagtaatca gcaagtttag ctgctagttc tcttcgataa acatcgacta
    19381 gaccgaacat ccgagaataa ggcatactag taggccctaa caaggctatt gtacctcttc
    19441 catgaccatc tatctcgtaa cttgcttgaa tcatactcat attctgcaat aaatcattgc
    19501 ctaattcagc accaatcttg atatgaatat tgctatccat cggagtgatc atttgtgtaa
    19561 gtccgtctga gtctttcata aaagaaaaca tcgatttgaa ctgatcaaga tcttgacggg
    19621 gctcataatc caagagattc atttgtccac tgacataaac cttatcttca aaaacatgac
    19681 caagaacggt gtcaaataac tgagacatcc cttctgtcgt ttggaaatat ttatgaagaa
    19741 tcatcggaat ctccgtacga agtttatggt acaccgtcat cagaggatct ccgactagac
    19801 ggtcgtttac aatctgtacc attttttcta gttcctgact tcccatatat tcgggaagag
    19861 tgaacacttg gctttctaca ttccccatat ctgttacgac gatagcaata atctgtcgat
    19921 tattcaaggg tacgatacga aagccagtca gtcggcggtc ttttatttca ggtcccaatg
    19981 aaaaagtcgt atagcttgtc agctcagata atatctcagc agattgctta ataatctcat
    20041 tgatttcatg aaattctctg cctagagact gacgaatcag cagcaaatca tcttctgcta
    20101 cgcgagcagg ttttaacaaa tgatccacgt aataacgata gcctaaagca gatggaatcc
    20161 gcccagaaga agagtgagtc ttcaacagca atccttcatc ttctaatgct ttcatatcat
    20221 tgcgaatcgt tgctgagctg gccttgactc cctcagacat caatgttttg gaaccaacgg
    20281 gcacgccact actcgtatag tgttggatga tcagacgtaa aatgtcgttt tgtcgctttg
    20341 ttaacatctt cctcacctct cattagcact catatattct aagtgctaat ccacttataa
    20401 atatagcaag aagcccttct cctgtcaaga gaaagactcc attttttagc actctaaaca
    20461 caagagtgct aattctaaat aattgatgta aatccagtta tatcaacgtt tcaatagtcc
    20521 tttattgtat ttttgaactt tttttattta aattcagata aaaaacaaat attctttaaa
    20581 ctatttttca aacaaaaagc gctcaaacac attgttcccc aaaaaggtcc ctcttggtgt
    20641 caaataaaca cggtcttctt cattaacaag taacccttct tgctctaatt cccttagcac
    20701 atcaccgtac accgattcaa ttgttttttg atagcgatca tagaaatgtt ttttagtaat
    20761 ccctgttttt tttcttagtc ctaagaacat ttcttcttct atttgatttt ttcgtgtcaa
    20821 tttttcttct tccaataccg ggcgttcatt tgctcgtaat gggcgtaaat agtgttggat
    20881 tggtcctttg ttgcggtaac gaatatctcc aagataaccg ctagcaccag ctccaaaccc
    20941 aaaatagtgg gcattgttcc agtacatgag gttatgttga gattcatgtc cggagagccc
    21001 aaagttactt atttcatatt ggtgacgtcc atgcttttcc attgcttcaa tcgtctcttc
    21061 aaacattctg gtttccgttt cctgatcagg cagttcgagt cttccttgtc gtacccagtt
    21121 catgaacatt gttttgtttt cgaggatcaa tgagtacaaa gaatagtgag gcagatccaa
    21181 agccaaagct ttttccaaag tatccagata tccttctagt gtttgtcccg gtaatgcata
    21241 aatcaaatcg atgcttacat tggtaaaatt ttctgcttct aaaaattgca tagtttgata
    21301 cacatcttct gcggtgtgtt tacgtccaat ttttttcaat aagcgattat caaatgtctg
    21361 cactcccatg gataaacgat tgacgccata gtttttcatt acttgaagtt tttcacgggt
    21421 caaatcacct ggattcgctt ctacagtaaa ctcattacca ttttgaaaag gcaggatctc
    21481 tctagcacct gataacagtc gatcaagttg tttagcagat aaagaagttg gtgtcccgcc
    21541 ccctacgtaa agtgtttcta aattcgctgt cggatgactt tctagcatta ttttcatttc
    21601 attcagcagc atttccacat actcatctac tggctgccct tctaaaaaaa ctttattaaa
    21661 atcacagtaa tagcagatat gctcgcaaaa tgggatatga atgtacgcag aaaccttgtt
    21721 ttctgcggga tttattgttt ctatcattga aattctttct ccttacgtcg aaaagtcgtg
    21781 cctgttttcg accttcttaa aatcgcgttc aagcaccaac tcattgaaat aagccgcata
    21841 tttagaatca aggaaaccta gctctaaaaa tgcttgctta ttgtcatcgg agctacacgg
    21901 tgcttttaca gcctctcgat aaacggtaat cgcaaagctg accttcggca ttaagtcaaa
    21961 atctgcaaaa atatgaaaag ctattttcac agattcctcc ttaagtgctc agaccacaac
    22021 gctttgctta caacctctca tatacgcata ctaaacgtat ttggcgaaca aatccactaa
    22081 aaaatccaag aatcgacttc ttggatgatt ttttctttca ttaaactggt tacctagtta
    22141 tatatctcta gggttgtttt catgtctgag tgcccgatat gatttatttt tcgtttctat
    22201 aattactttt tgccttccag tttcttttaa tattgtactc aagcaagtgg gaattggttc
    22261 ttttgtcatc catgtatgca aaatgttata atgctggtaa acaaaaatga ttacctgtca
    22321 tttttattta gttcgtattt aattaagttg actatctgta tactctgtct aaaacttaag
    22381 aattgttgtt ttcgctgtcg ctacccaatt ttaatgaatg tctgtaggtt aaaactgttg
    22441 tccttgttat agatagtaaa ggagagcttg aaaatgaaaa ctgcgattac gtttctatta
    22501 gaggaatatg ccgattggga aggtgcttat attactagca cactaaacca aaatgagaaa
    22561 tggcaaaaca aaactgcttc tatccaatca gaggtcactt ccattggagg attaaaaaca
    22621 aaaatcgatt acttaatcga acaaatacct gaaaaagttg acctttttat tatgatcggt
    22681 ggcaattcat gggacataaa aaatcatcat ttatatcaaa taatcgataa attgttaaaa
    22741 acagacgtac cagtagctgc tatttgtggg gcagttgatt atttagctca gagcggatta
    22801 ttgcaaggtt acaaacacac cggaaatgcg caatttcctt ggttagaatc ggaaaattat
    22861 cagaataaag aagattttat agaacaacaa gctgtgaaag atcgtaattt gattactgcg
    22921 aatggaacag ctcctataga attcacagac ttgattcttc aagcagtcca ttttgaaaca
    22981 gaagaaataa gagaaaaaac gattcgtcta tacaaacttg gattctatga ttactataaa
    23041 aagtacggag atcctttctc ctaatcattc tcttcaatta aatactcctt atcaatagtg
    23101 tgacaagtaa ttttactgat gattatttgt cacacttttt ctgacattta tcaaagaaga
    23161 tacactctat ttatattcat agacaaggtt ttacggattc aaaaattcag gattatgcta
    23221 tactggtatt aaaaaaataa tgaggtattt tatatgaaaa agttaggttt tattttattg
    23281 ttggtctctt tattccttgt aggatgtacg tctcctagta aagaaaatag tgatccatct
    23341 gcgaccacag acaaaagttc aatcagacaa accagttcag ctgaaaaatc aagtactcag
    23401 aaatcagctt ctactgaaac gaatacatct tcaacaagca gtaaagtctc agagcccaat
    23461 acttcttcta catctagtag tacagttgag acaaatattt cttcttcaac aatagacaca
    23521 acctcctcac agtcttctca gtcaatagat ccgttagcag gatattctga tctacaaatt
    23581 gagtacgcac gtgtttggct agctattaga ggtaatactt ttagagatac atcgggtgat
    23641 ttcgaattac atgttattca ttatcctgca ggaacaccta tcgcccctta tgatgcaggg
    23701 agtgcagtct atccgaaaga tgtggttatg ctcactggga aatacagtta ccagggagtt
    23761 ttagtgtatt caagtaacca tgacgggaca atcactagtt atccagttcc ttcacattgg
    23821 caaatgcctg cagatcaaac atcttatcca gcgttcatta gagaaacaac tcaagaaatc
    23881 attgataatg catctgtagt agctattcct acaggaaatc ccaatgacgt gaaacagtta
    23941 atcgatgtga cagttataga taattagact ttctaatatg ttatacaaaa agaagctgac
    24001 agcattttgt cagcttcttt cactttcttt tctatttaga tttattggtc tttgatagac
    24061 tccaaggcag gaaaaaattt tttaaataaa cgtttgcaaa acagtcgctt ttctatcttc
    24121 taccgctact tctgcatagg caccattaat caatgtcagt tgtggcggta catgaccaca
    24181 atcaatatca tacaagatcg gtagttttaa ttcctttgac agatcttcgt atacatcctt
    24241 cattttataa tctaacaaat cctctgccgc acttctaccg aacatgatcc ccgagctgcc
    24301 atcaaaccag cctgccagtt tcatctgaac aagtgatctt cttaaatcaa ctgacgatag
    24361 ctcacagttt tctagaaacc acaagatcgg ctttccatca ttatactttc ttttaaaaga
    24421 ttggacatct ccataaggag taccgattaa atgcctgatc gtatcaatac aaccacctaa
    24481 taatcgtccc ttcatcctta ctgcatctcc gctgactgat tgccaatttg ttggttcagt
    24541 gagatggaaa acagaagatg atgaatgctc atggggccac actttttgat acttttcaga
    24601 agattgctgt agaatcgatt ctccttctct tgttttcagt acactttccc acattgctgt
    24661 cgtttcatcc aagtactctc ctcttaaatc aataaggtta gttccatgtg cagtggctat
    24721 ccctgttttt aatgtgacag caagtagcaa cgtactaata tctgaataac ctactatcca
    24781 ttttggttgt agagcatcaa aatcgacaaa atcgagaatc tcaatcaata attctcctcc
    24841 ccaagtcgga aagatcattt ggatattttc atcttgcatc atttgattga attcatctgc
    24901 tcgttttctt gctggacctg atttttcctt atcttgcgtc caaagcgtct ttccagtaac
    24961 tacttggaaa gatttttctt ccattctctt taaagctgat tcgagtaacg aatggtgttc
    25021 ctctgatacg cctgaggatg gtgcagtaac cccaatcgtt gcatttcttt ctaataatgg
    25081 ataacttatc atgttgcttc acctccaagt atgcactatt ttacttggtt aattgttttt
    25141 ttcgtttgcc ggcatcaaaa atatcggcat ctcaagtagc tggatgattt tgtttaagtc
    25201 aaccgtttgg atgaccatcg tttgatctgg tctacctggt gcaaaaacaa gtttttcatt
    25261 tgataagagc tcttcatcaa taataatcgg aatctgtttt tcataaccaa acggagaaac
    25321 agcgccctgc ttttgtcccg tttgttcttc catgcgttct gatgaaacaa tagaaagcct
    25381 tttaccgacg atcttcccta atgctttgaa atccgtttgt ttcgtagtaa aagtcagata
    25441 tacgtagtta ttatcttttt tatcttttaa gaataagctt ttggtctctg tacctgtaaa
    25501 tccttgttct ttctttatta ccaaagcatc ttcatttgta taaatggctc gatgatgata
    25561 caactcaaaa tatatatttt cgtgtaagaa tttctttgcc aagtcaatca tttttatgtc
    25621 tcttttctat tttattattt gagacatctt atcatataaa tatagcggtt acaacattac
    25681 ttgtgttttg ttcgtttttt atacataact attgtttttc tggtaaaata aatcaaacca
    25741 cttaatggag gcgttttaga atggaaaaat ttcagccgat catcaatcaa atcaaagaaa
    25801 aagaacatca acaaaagatc aaagaccttt ttgctaagat aacagaaacg tatccccagc
    25861 tagacacaac tgtaaaatgg aaccaaccta tgtttactga ccatggtacc tttattattg
    25921 catttagttt tgctaaaaat catttttcta tcgcaccgga aaaagcagcg attcgtgccc
    25981 ttgaaaaaaa tatccaagaa gcaggctacg tatatacgga taacgtcatc aaagttcctt
    26041 ggaaaagtgt aatcaactgg gaattgattg aacaattgat ttcatttaat attgaagata
    26101 aaaaaggtca tgataaattt tggcgataaa gctttatacg aataaacctt ttactggcga
    26161 ggaaactatt catcacctta tacaggtttt gttcaatcct atccttttta tcatccctgg
    26221 ttaaacactt aattgacaaa gtatctgcta tttttataag gagtctttct tgtgctgaaa
    26281 aaaatcactg attgaatggc tttgaatttt ctacttagta aaattatttt ttcagcaatt
    26341 ttcctgcaaa acttttgaat aaggaaaaaa tacgagaaaa tatcagtgat ctttcataat
    26401 actgcccatt attttcaggt atatttaaat ataatatcac tttaggagag aacgaatttc
    26461 tttagagaaa taaaagttag aattttctga cttgattttt acaatgacct taattaaaat
    26521 cgttctttct acatttcgtg attaagcaaa tatcaactaa gtcagtagaa ccattcacat
    26581 aggagggtga acattgaaag atttttactc agtaaatgaa ttagccgaac aattaggtgt
    26641 tactacacgt tctatcagaa attatcttca tgaaggaaaa ctaaaaggaa caaaggttgg
    26701 cgggcaatgg aaattttctg aacgtaatct ttttgagttc ctatacggag atcaagcaga
    26761 ggaagcagca aaagaaatgc agcgattcat gctaaatgca ccaatcacga tgcgtttcaa
    26821 ccttcaatat cgtgatttta cagcaatcaa tcaattccga gaacaattag tccagtacca
    26881 taacgatgtt tacgcaaata aaaaagaccg tcttttacaa tacgatcttt ataaagacaa
    26941 tcatgcggag atactaatcg gtggtaactt caattatgtc gtaaacttta gtcaatggat
    27001 caatgaaaaa ttattgatcc agacagatat ttcccttgta tcttaaacaa ttaaaaagcg
    27061 ctagaaattc cagagatgtt ggaaattcta gcgctatttt tttataagta cttactgttt
    27121 ggttcaaaca aatatttttt taataaatga aattttacca tctttatcct caaataaaga
    27181 ccgtgaccat gcttcctact acgatcagca ataatccgat aatcgttaat tttttttctt
    27241 ttggtgtctt attttcatgc aggatgtaca taccgcctaa tgtagagata actacgttta
    27301 gctgtgtaat gatatatgca gtagcaaccc cattttcatt tgctgaaaaa atataagcca
    27361 atgctgacac actaaagatg gctccaacga tcgttgcttt ccaactcttg tcttcagaaa
    27421 atgcggttgg tttacggaaa atcacataaa tcactgcccc taaaaagaca ccgatcattt
    27481 gcggaaagaa aatggaaagt ccgcttgcat caactagttt aggcaatgca ctgtataccc
    27541 aataaccaat acttgttgat gctaatagac taattccttt tgtgataccg cctttttttc
    27601 caggatcatc actgattgcc gttaaagcag aaccaaatac aagaagaatg attgctgctg
    27661 ctccaatgat tttccccata cttccagacc attctccaaa agcgaagaca ccaatcaacg
    27721 aggttccgac taactgtaat gcagtcgaaa ttggcatcgt tttagaaaca ccaatcaagt
    27781 tgaaagatgt atactgtcct gtttgtccaa taacccaaaa tgcaccagaa agtaaactaa
    27841 tcaaaaatgt agtcactgaa ataccagctg gagaagcgaa taattgaacg agtaagccga
    27901 caagcaccgc tccaatacca gttcctaata tctcatttgt tggtctgcca ccaatctttt
    27961 tcaaaatcag tggctggatc ccccagccaa ctgccgggat caatgcaatc aataaaccca
    28021 tttttattca cccttatcct tttccacctt ggaaagaagg atataaagtc atcccaccat
    28081 ctgcaaatag tgtaatacct gtaacataac tggattcgtc tgaagccaac catgcagctg
    28141 tcgccgctat ttcttttggt tcacctacgc gtcctagtgg gatcatactt tccaagagtt
    28201 ctttttgttc aggatcatca aattttgccg cattaatagg tgtacggata gcaccagggc
    28261 ctatactatt gattctgatt cccttcggtg catactccat tgctaccgtt tcggtaaata
    28321 attttacccc gccttttgaa gcagcgtaat gagcaaacgt tggccatggg atctgttcat
    28381 gaacagaaga catattgatg atatttcctt ttttattatt ctctgtaaag taatttaaag
    28441 ccgctttcgt tccgataaaa acgccagtca aattgacttt taacactctt tcccaatctt
    28501 caaggctaag ttcatgggtt ggatgctgat tttccatccc agcattatta acccaaacgt
    28561 ccagtgtccc aaaattttct aaagccgtat caagtaaacg ctgtattcct tcttcagaac
    28621 tgacatctgc ttgtacagag actgcttttc cgcctgcctc ttcgattgct ttgactgctt
    28681 tttctgctcc ttctttatcc gagttatagt tgacaactac attcattttt tctttcccga
    28741 atcgttgagc aatcgctgtt ccaatcccct ttgagccacc agttactact gcgactttgt
    28801 tttctaattc cttgtacata gtacccactc ctttttcata cctaaacctg ttattacgga
    28861 taacttttat ttccgcctta aagtgagtgt acaccaagat atatagtcta aagcaaatga
    28921 tacgctctag caatcgtttt catgaattgc tagtgtcctt ttatttctat aaagaaagag
    28981 gttgtgacag aagtggtcag cttcaagaaa tttcggctta tttccgaaga agctacttct
    29041 gaaaaaccat ttatttggaa aaaaaggctg tgacaagact tttgtcacag cccctttcct
    29101 gggcttattt atcacccatt ttattgtaat tgctagttat acataaacaa gagattttat
    29161 gcataatgtt ttattgctga gaaaaatatt cagctgttgc ttgttcatcc tccttgagtt
    29221 gttcaatcaa ttcctctaca ctagaaaatt tcacttgatc tcttaaaaat tgattccagc
    29281 gtaccgcgac atgttcacca tagatatctt gattaaagtc caaaatatac aactctacag
    29341 tcagctcccg tccttcaccg aatgtatcat tatgtccaat tgaacccatg gctggatacc
    29401 atgtttcccc aatttttatt tccgacacat aaacaccttc tttaggcaaa tgaaccgtgc
    29461 tctttacttt gacatttgct gtagggaatc ctagtagtct gcctcgtgca tcgccatgga
    29521 caactacccc ttctacctca tatgtgcaac ctaatagttt tgctgcttct tttacttgtc
    29581 cgctgtctag taatccacga atacgggtag agctgatttt ttcaccttgt tcttcttctt
    29641 tagaaactgt gacgattttg aatcttcctt tggcatattc aggaaaatga gcgacatctg
    29701 caatgtcttt tggtccatac gtataatcaa aaccggaaac agcatatttt gcatgtaacc
    29761 ctacgatgta ttgatcaaca aattcctgcg gtgctaaatg agcaaaagaa gaagtaaaat
    29821 caacttcata taaataatct atacccaaag cggccatttt ttcttctttt tgcttaaggc
    29881 tagtcaaata tttcacgctg ctaggatcga tctttttaaa gacaatagat ggatgctgat
    29941 tgaaagtcat caatgctaat ttcaatcctt ctttttgtgc aatttccata cctgtcttta
    30001 tgactttctg gtgtccgata tgcacgccgt cgaaaaatcc cagcactaaa acgacatcgt
    30061 cttctggaat ttgttctgat tggtaaggat gtctgatctt gatgatattc attcgttatt
    30121 cctcatttct caatacttta ctaggtttta atttgttttt ttctttcggg tttggctgat
    30181 aaatactgac aacttttcct ttataaaaca aagcaatttc ttctgacggc atttctggga
    30241 gcgcaaatac agtataatca agtctcatac cgttttttac tttctgccat acttcatcag
    30301 taatatctac tcgtttgaac cgttccactc ctttttctat tggcagcaaa caatcatcga
    30361 ttgtcccatt tttcacagct tgagctactt cttctaatgt cagagcatcc ttctgggaca
    30421 taccggcact tgctgttcgg gtcagatcag acatgtgcgc agggtaacca agtttcttgc
    30481 ctgtatctac agctaatgtg cggacatagg ttcctttccc gcattctact tcgaagcgcc
    30541 aagaaatggt tcctgtcact tcgtctcttg tcaattcact tgtccggata aaacggtaaa
    30601 tctgagcctt tctaacagga cgctccactg tttcattatt gcgggcatat tcatataacc
    30661 gcttaccgtt gacttttacc gctgagtaca ttggtggtat ctgggtgatc tcaccaataa
    30721 atgaagccat cacttcatct attttttttt cagataaaga catatctact ggttttgttt
    30781 ctacccgttc tccggatcga tcttccgtcg ttgtactgaa acctagagtt atctcgccta
    30841 catagatttt cccagaatct gtcaaatatt cgataacttt tgtggctttt ccaatacaaa
    30901 tgggcaacac accgtctacg tctgggtcaa gcgtaccccc atgtccgatt tttttcgtat
    30961 gtaagatttt tcttaatttg aatacgcaat catgacttgt cattccgcgt tctttccata
    31021 aaggtaataa cccgtccatg tcttttctcc atttcatttt tgatttaact gaattattat
    31081 aacacattgc atcaattact gacgaatttg acatcataat gaacggtttt tatttttcgg
    31141 caataaaaac gtaacaaaat tcatgctcac gtaaatagaa atcatgttat gcttttttaa
    31201 aggagggaat agaatggaag attttttctt tgcaccattt gattggattt atgggtggct
    31261 tacaggtaaa tagtttggtt ttcacttccc aaaaacgaaa aaaaagactg gccattcttc
    31321 cagtcttttt tccgtttttt ttatttattt tttcagtttc ttaatcaatg taaaaaattc
    31381 tattttgcgg gatcgatttt atcattttat tggtcaagat ttttccattt ccaatccaat
    31441 acttctggca gatcaatacc attttcacga atatagtttt gatgttcttg taatttctga
    31501 tccatttttt tcgcaaaatc ttctgcttga cttcctttga tttttaaagc tgcgtctttt
    31561 gccaaatgat aacgatcaag ttcatttact acacgcatgt caaatggtgt cgtgatatct
    31621 ccattttctt tgtagccatg aatcattaaa tgatggttat gacgatcaaa gaaaatcgtg
    31681 cggaccaatg tttcataacc gtggaaagca aataagatcg gtttgtcttt tgtaaaatat
    31741 tgatcaaatt cttcatcgct caacccacgc ggatcttgac ttggatgacg taatttcaat
    31801 aaatctacga cattgatgaa ccgaattttt agttttggga attcttccag cagcaaagtt
    31861 acagctgcta atgcttctaa gttagattcc gttcctgcag cagcaatgac caattcaggt
    31921 tcctcctctt ttgctgtcga agcccaatca atgattttca agccttcatt cactaagacg
    31981 gctgcttctt ctgctgagta aaattgagca cgcggatgtt tggaagagat gatcaaattg
    32041 attttttctg acgagcggaa tgctttatcc atgactgcga gcaatgtatt tgcatccgca
    32101 ggcagatatt cacggatgaa ctccgccttc ttttctgcca aatgtgtcaa tattcctgga
    32161 tcttggtggc tatatccatt gtgatcttgc tggaagactg ttgagcttgc aatgagattt
    32221 agcgaaggat aatcatttcg ccaagaaagg tcatgtgatt tccgcatcca tttgaagtgc
    32281 tgagtcaaca tagagtcaac cactcgcaag aatgattcgt aacttgcaaa aaaacatgcc
    32341 gtccagtcag aacgtaccct tctaagaacc cttcatcttg gtgttccgat aattgactgt
    32401 cgatgacgcg accagatggc gaaagccatt catcattttc tggttcgatt ttttccatcc
    32461 attggcgatt tgtcgttttg aaaacttgat tcaatcggtt tgattttgtt tcatctggtc
    32521 caaatatacg gaaattttct ggatttttct caatgatctc gcgaacgaat ttaccaagtt
    32581 cggtcatatc ttctgcttta acggagcctg gtttttcaaa ttgcaaagta tattttttcc
    32641 aatctggcat aataagcggt cttggatcga ttccaccatt ggtgatagga ttttttgcca
    32701 ttctttggtc accagtaggc gcgatttctc tgatttcttc taaaatacgt ccttgtgcat
    32761 caaataattt ttctggttca taggatttca gccaacggag taaagcatct gcatgatcca
    32821 tatgttcttg atctacagga atcggtactt ggtgtgctcg gaaggttcct tcaattggtt
    32881 ccccgtccca ttcttttgga ccggtccatc cttttggtgt gcgtacgatc aatactggcc
    32941 attttggcat cacgacatct gttgctgtct tcaggcgagc ttctttttgg atggatttaa
    33001 ttttctcgat tgcttgatcc attttttcag ccataacagg atttaatttt tctgaatcat
    33061 ttccttcaat aaagaaaggt tcccagccaa gtccgttaaa gtaatttgct aattctccat
    33121 ctgatttacg tgccaagatc gttgggttgg ctatttttgc accatttaaa tgaaggatcg
    33181 gtaaaacagc tccatctatc actggattga taaagacatt tgagaaccag cttccagcta
    33241 aaggaccggt ttctgcttca ccatcaccaa tcacgacagc agagattact tcaggattgt
    33301 ctaatacagc tccgactgca tgtgaaagag agtatccaag ctctccgcct tcatggatag
    33361 aacctggtgt ttctggtgct gcatgagaag caatgcctcc aggaaatgaa aatcgtttga
    33421 ataatttttg cattcctgtc tcatcttccg ttacttctgg atagatctct gtgtaacttc
    33481 catctaaata agcattcgat accatgactt gtccgccgtg tcctggccct tcaatataaa
    33541 acatgttcaa atcatattta ttgatgactc ggttcaaatg gacataaata aaattttggc
    33601 caggaatcgt tccccaatga ccgattggat gttttttgac atcttcgggt ttcagtgtac
    33661 gtctcagtaa ggggttatct ttaagataaa gttgcccaac agaaagataa ttagcagcac
    33721 gccaccaagc cgtcatttta tcaaagtatt ctttactaga ataatccata cataacactc
    33781 cttttcatta ttactcttat tctagcgcag atactcccaa ttgaaaccta ataagcactt
    33841 ctgttcattt gtttggattt tatatctagc caaatgaaca ggtataaaaa accacgaaat
    33901 ttttcctttc gctgacgttt tttacattga tccaaaaaac ttgtcgttat cacaatggct
    33961 gttccgcagt tcatttcgct cctcatcatg cataatttgc tgaatgacgc tgggccagtt
    34021 aacacattgc tggaaaactg gggctggatc gagcaattcc tttcctgatt gatccgtttt
    34081 atgtaaaagt ctctgttatc tttgtcaata tgtgaattgg tatcccagct actatgctaa
    34141 tttctacagg gattatccaa aatttggatc gtgaccaaat cgaagcggcg taatcggaaa
    34201 tatcaataac ttcaacgtga tttttctttt aactgtcaac ggaccgacaa actcaaactt
    34261 ttatggtgca ggttccactg atcttctagt tacttggcta tacctgactg tcactaaaat
    34321 agattacaat ctagcttccg ttatcggtat cttgatcttt atctatatgc aaaaatttat
    34381 atactactag aaaaaagaaa gtcgaatttt ttacttttct ttttctcttt tttggttagc
    34441 tgacacgccg caagactaaa gtcatggaac tcttaggtaa tacgtcttaa tgaaaacaaa
    34501 aaaatcccgc aacccctgat ttcacaggat ttgcgggagt ttttgattag tctttgttca
    34561 agtcgcgaag caattcatcg atatggttgc catagacaac tgattcatca agttcaaaga
    34621 acaattcagg tgtcttataa atacttagat tgtgccctaa ctctcgacga atcaatcctg
    34681 atgctttatt caatccatct tgtgctgttt tcttatctga tgctaagtca gataaaatgc
    34741 tgtaatagat cgttgcttgc tgtaagtccc ccgtaacttt cacatctgtg atcgttacgt
    34801 tctccacacg agggtcacgg acctttttgc gcaaaatatc attgacttct tttaagattt
    34861 cttgaccgac tcggcggtca cggtagttag ccataatgat tggcctcctt ttatcagttt
    34921 ttagtcattt ttgacttctt ccatgataaa tccttcaatt acgtcatcta ctttgatgtc
    34981 attgaatttt tcaatcattg caccacattc ataacccatt ttgacttctt tcacgtcatc
    35041 tttgaatcgt ttcagacttg caagttggcc ttcatagatt acgattccgt cacggattac
    35101 acggacaccg ctatctctac gaatatatcc ttctgttaca taggcacctg caatcgtacc
    35161 aactttagaa actttgaagg tttcacgaac gatcatttga ccggtgattt tttcttcaaa
    35221 ttcagggtct agcatccctt tcatcgcagt ttcgatttct tcgatggctt tatagatgat
    35281 acggtgaaga cgaatatcga cttcttcagt atcagcttgc acttttgcct gcggtgtagg
    35341 acgaacgttg aaaccgatga taatagcgtt gctggctgct gctaaggtta catcactttc
    35401 attgattgct ccgacagctg aatggacgat ttttacacgg acaccttcca catcaatctt
    35461 cttcaatgat gcagccagtg cttctgctga accttgtaca tctgctttga tgattacgtt
    35521 aacttctttc aattcgcctt ctttcaggct ttcaaataag ttatccaaag tcacacggct
    35581 tgtggcagca cggttttcta ataatgcacg tttcgcgcgt tcttcacccg ctgcacgtgc
    35641 tgtcttttcg tcttcgaaga ctacgaaacg gtcacctgct tgaggtacat cattcaagcc
    35701 agtgatttct actggcgttg caggtcctgc ttctttatcg cgacggccaa gatcgtttgt
    35761 cattacacgg acacgtccgt aagtattacc gacaacgatt gggtcaccaa catgaagtgt
    35821 tccttcttgg acaagtaagg ttgacacagg acctttacct ttatcaagac gtgcttcgat
    35881 cactgttccg attgcgcgtt gtgttggatc tgcttttaaa tcttctactt ctgcgactaa
    35941 caggatcatt tccagcaatt cttcgatgtt ttggccgaat ttcgctgaga tctcaacaaa
    36001 gatcgtttct ccaccccatg cttcaggaat taactcatac tcacttaatt cttgcataac
    36061 atgttgaggg tttgcacctg gtttatcaat tttatttaca gcgacgatga ttggaacgcc
    36121 tgcagctttg gcatggttga ttgcttcaac tgtttgtggc atgacgccat catctgcagc
    36181 aacaactaag atcgtgatat ctgtgatact tgctccacgt gcacgcatac ttgtaaaggc
    36241 cgcatgtcct ggtgtatcca agaaagtgat tggtttacca tcaatatcga tctggtaggc
    36301 accgatatgc tgtgtgattc cgccggcttc gccgcttgtt acacgtgaat ggcgtaatgt
    36361 atccaataaa gttgttttcc catggtctac gtgtcccata atcgttacga ctggcggacg
    36421 tgagactaat ttatctggat cgacttcttc tgcctcgaag aatttatcga tatcagcaat
    36481 gtcaacttgg actttttctt gtggttccat gccatagtcc gtcgctaaca gttcaatcgt
    36541 atctttatcc agtgcttggt tttgattgac catgacacca agcataaata attttttaat
    36601 gatttctgct ggctcacgat gaattttttt cgcaatatct gctacgttca taccttcagt
    36661 atactctaat acatctggca attcacggaa tttgcgtggt ggtacagctg gtttagttga
    36721 ttgttgctgt ttgccttttt tacctttttt attataacga ttacggttat tattattaaa
    36781 tttgccgcga ttgttattgt ttggtttgtt gttattgtta gtacggtttt gattgccgcc
    36841 ttggctcgtt ccttgacgat tttggctgct ggtatttccc gtattatttt ggtttgttgt
    36901 tggttttttt ccttgattca cttgaccgct cccttgaccg cgatcttgat aactgcggtt
    36961 atttttattt ttttgttgat tgttcgttgt ttctttttta cgttgatcgt tcggctgttg
    37021 attggctggt tttctgcttg cttgttgtgc agcttgttgt ttcgtgcttg ggtttttgaa
    37081 cgcttgttgc aatttcttct catcattgct agtaatcgca cccatatggt ttttcacatc
    37141 gatgccaagt tgttgtgctt tttccacgac gtctttacta gattttttga gttcttttgc
    37201 taattcatag attcttttgt tgcccatgca atcaccttcc taaccttcaa ttagctcctt
    37261 ggtcttcttc gcaaatccgg catccattat tccgaatacc ttgcgaggtt ttccgatcat
    37321 ctgcgtgatt tctgcttcag aaaatagctc aaagcaagga acttcataat atgaactctt
    37381 atcttttatc ttttttcttg tattctcgct tgcatcggtt gccacaaaca ccaattttgc
    37441 tttttgtttg cggatttcag caatggtcag ttcttcaccc gtgattagtt ttcccgcgcg
    37501 catcgctaga ccgatgagat tcatggcttt ttgccgatta actgtattca ttttccaaat
    37561 aactctttac gtgctttttg gtgagtgaca tagtcaagaa gttcttgata aaactcatct
    37621 gttaatgttg tttcgagtac tcgatcgaga atttttttat cccaagcttg ttgtacttct
    37681 tccggctcaa gtgagacata cgctcctcgt ccaggcattt ttccagttgg atcgatcgat
    37741 acttcccctt cttttgaacg ggtgattcgg accatctctt ttttaggttt catttcacct
    37801 gaaaccacgg atttgcgtaa aggaattttc ctttgtttca tctctttgcc tccttactaa
    37861 agttaagctt ctggtttttc ttcgaccgtt tcattgttga atgcaattgt ttcatattca
    37921 tcgtctgtta aatctgactg aacgattgct tcatcatgca gttcttctgt ttctttttgt
    37981 tgttcttgat tttcataaaa ttccgtcatg tcagattctg atttgatatc gattttatgg
    38041 ttagtcaatt tagcagctaa acgtgcgttt tgtccgcgtt taccgattgc tagcgataat
    38101 tggtagtctg gaacgacaac tgtgcaggcc tttggatttg tctcatcgaa gatcacatcc
    38161 atgacttgtg caggattcaa tgcatttgca atgaatacag ctggatcttc attccattcg
    38221 acgatatcca tgttttcgcc ttttaattca ttgacgattg cttgaactcg ttgtcccttt
    38281 ggcccgacac atgtaccgac tgcatcgata tttggatcat ttgagcgaac tgccactttt
    38341 gaacgatcgc cagcttcacg ggcgatgctg acaatttcaa cgattccatc atagacttct
    38401 ggtacttctt gctcaaataa gcggcgcaat aggtctggat ggctgcggct aacaaatact
    38461 tgcggacctt tagacgtatt ttctactcgg gaaacataaa ctttgattcg atcatgtggt
    38521 tgataaaatt catttggcat ttgatcttgt ttagaaagaa ctgcctcaat tttaccaaga
    38581 ttgacataaa tataacgttt atcctggcgt tcgacgattc cttgcatgat gtctttttca
    38641 taagcactga attcgttata aataatcgtt ctttctgctt cacgtacacg ctgtaaaata
    38701 acttgtttag ccgtttgcgc agcgatccgc ccaaaatctt ttggtgttac ttcaaaacgg
    38761 atcgtatcac cgatctcata tgctggattg attaaaagtg catctttcaa tgagacttcc
    38821 aactgtgaat ccatcacttc ttccgttact tcttttaccg catagacatg gatctttcct
    38881 tttttctgat caaattccac ttcgacgttt tgtgcttggc catagtgacg tttgtatgca
    38941 gaaaccaatg ctgcttctaa agcatcgatc acgatttctt ttgagatccc tttttcagct
    39001 tctaaagcgt ctaacgcatt taacatttct ttactcattt tttctgattc ctctccgtga
    39061 ttaaaattgg atagctaagc gagcttttgc aatatttttt cggtcaaaag tcaattcttt
    39121 ttctcgtgtt tttatgcgta ttttcaatgt aagctgttca gcatcaaatg attgaagaaa
    39181 tccttcatat tgtttttcgc catctactgg ttggtataag gagacatgga cataatcacc
    39241 aagtgccttt tcatagtcgc tttctttttt caatggccgc tctgctcccg gggaggatac
    39301 ctccaggaaa taagcttgag gaatcggatc tggatctgct gcatcaagtt tttcactcag
    39361 tttctcactg acaaaggcgc attcttcgat atcgataccg ccttctttgt caataaatac
    39421 tcgtaagaac cagctttttc cttcttttac aaattcgact tcgacaagtt caaaattttg
    39481 ttcatctaaa atcggtgtca ccatttctgt gactgtttca acgacgctac tcaaaagttt
    39541 cgcctcctta aaaaattaag tgcgtaattc cattaaaaag agtgagcgaa aaatccgctc
    39601 actcacagtt agtatcatta cctataagaa ctataccata ctctgcttaa aattacaagc
    39661 agacaggaaa acagatgctt atagcatatc gaataacgat aattgatttt catctggtag
    39721 atctttcaat acgccattgt cgttcatata ttcgatcaat gtttttgaaa ctttaccgcg
    39781 ggtggcaaga tcttctttgg ataaaaatgg accgtctttt cttgcttcaa cgatttgttt
    39841 tgccacgttg gttcccaaac ttgggacagc tctaaatggt gcgatcaacg tatcaccttc
    39901 aataacaaag ttaacggcat ccgatttata caaatcaatc attccaaatt tgaatcctcg
    39961 ttccagcatt tcattggcta actccagcac tgtcagttga ttcttttctt ttacggaagc
    40021 atctaatcct ttgtccgtga tttctttcat tgcttgtttc acagcatctt ttcctttgca
    40081 catggagact agatcaaagt cgtctgcacg tacagaaaag tatgcacagt aatacaaaat
    40141 agggaaataa accttgaaat aagcgacacg aagagccatc aatacgtaag ctgcagcatg
    40201 ggctttcgga aacatatatt tgatttttga acaagaatcg atataccagt cgggcacatt
    40261 attttctttc atcgccgata gataggtttc tcgcaattca tcagggatct tgttccattg
    40321 tcctttacga acagtttcca tgattttaaa tgccatcccg ctatctaatc cagcatggat
    40381 caaataaacc ataatatcat cacgacagcc gattacttcc gccagtgtcg catctcctcg
    40441 tcgaatcaat tcttccgcat ttcctaacca tacgtctgta ccatgagata gtccggaaat
    40501 ttgcagtagt tctgcaaaag ttgtaggatg cgtttcttct agcatccctc gaacaaaacg
    40561 agtgccgaat tctggaatac ctaatgtccc tgttttggaa taaatttgtt cctgacttac
    40621 tccaagtact tcaggaccgg caaatatccg catcacttcc gggtcatccg tagggatcgt
    40681 ttgtggatcg atccctgata aatcttgaag catccgaata acagttgggt catcatgccc
    40741 tagaatatct aatttcaata cattatcgtg aatcgagtga aaatcaaaat gagtcgtttt
    40801 ccattccgaa ttttgatcgt ctgctggata ttggatcggc gtaaaatcat agacatccat
    40861 ataatcggga ataacgatga tcccccctgg atgctggccg gttgtccgtt tgactccagt
    40921 agcgcctttt gctaatctgt cgacttctgc acttcgaaac tgcagattat tgtccctttc
    40981 gtatcctttt acatagccat atgctgtttt gtcggcgact gtaccaatcg ttccggcacg
    41041 gtaaacatag tcttcgccaa acaatacttt cgtatagtta tgggcttctg cttgataatc
    41101 acctgagaag ttcaaatcaa tatcgggcac tttgtctcca tggaatccta aaaatgtctc
    41161 aaatggaata tcatgaccgt ctttgtttaa gcgtgcacca cattttggac attgtttttc
    41221 tggcatgtca aatccagaac catacgtacc atcttcaaaa aactcggaat attggcactc
    41281 tggacaataa taatgagggg ctaacggatt aacttcagtg attcctgtca tcgtcgcaac
    41341 aaagctagag ccaacagagc cacgtgaacc tactagatag ccatcttcat tacttttatg
    41401 cactagtttt tgggcgatca gataaataac cgagaagcca ttaccattaa tagaattcaa
    41461 ctcttttttt aatcgttttt gaatgatctc aggtaacgga tctccgtaca tttgttttgc
    41521 ttttgtgtaa ctcaattctg atatttcatc ttctgaacct ggaatcttcg gtgtataaag
    41581 ttcatctttg actggaatca cttcttcgca gatatctgcg attttattcg tattttctac
    41641 tacgatctct ttcgctgttt cttcccctaa aaagtgaaat gcagttagca tctcatcggt
    41701 tgttcggaaa tggacatccg gcaaactatg acgattcaac ggattcgctc cgcccataga
    41761 attgatcaaa atttttcggt aaatggcatc ttcttcgttc agataatgta cattacctgt
    41821 tgcaacaacg attttatcca aactttttcc gatttctacc aaattttgga taatttcttc
    41881 caaatcatgt tcatttttta ccaattcttg ctcgatcaaa ggcgcataga ccgcttttgg
    41941 cataacttca atataatcgt agaattttgc tcggtttctt gcctcttcta cccctttttg
    42001 catcatggct tcgaatattt ctcccttatc acaagctgag ccgatcaata gattttcacg
    42061 catctttttc aattgggagc gtggtattcg tgggacgcgt tcgaagtatt cgacattcga
    42121 cattgaaatc agtttgaaca aatctttcag tcctgcctga ttttttgcta aaatcgttac
    42181 atggaaaggt cgagcccttt tgtaagaatc tccttctccg atatgctcat ttagctgatc
    42241 atgataaagc atgttatgat tgtccattgc ctctttcaca aagatccatg ctaagtgacc
    42301 agttgcttca gcatcataga ttgctcggtg atgctgttcc aagctgacac caaatttttt
    42361 agataacaca cctaatccga aacgtttgaa ttgaggatac agatatctag ctaattccaa
    42421 cgtatcaatg actggattgg ccgcttcagg aataccatac cttgcgtagc tagtattcaa
    42481 gaaccccata tcaaaagccg cgttgtgggc taccaaaatt gtatcttttg aaaattcaag
    42541 gaacatccgc aatacttctt cttccgactt tgatcctcga accattccat ccgtgatacc
    42601 agtcagatct accgttgttc gtgacaaagg atgtcctgga tcgataaatt catcaaagct
    42661 ttcgatcaca tttcctttat acattttgac tgcggctagc tcgatgatcg tgtcatatac
    42721 tgcagacaat cctgtcgttt ccacgtcaaa tacgacatat gtggcttcac tcaatgcttc
    42781 atgtgcatca ttataagcaa ttgggacacc atcatctacg acattcgctt ccacgccgta
    42841 taagattttc acacctgctt tttttccagc actatgtgct tccggaaagg cttgcgcacc
    42901 accgtggtct gtaatggcga tagctcggtg tccccatttc cctgcttgtg ccacaagatc
    42961 agagatgctg tttgttgcat ccatcgtact catattgcta tgaacatgaa gctctactcg
    43021 tttttcccct tcaggagcat aatctttgcg aggtgtatgt ttgacttcta tgatatcttg
    43081 cgcattcatg accaaatcac ggacaaacgt atcttcttga atacttcctc gtacctttaa
    43141 ccagctaccg acactgatcg catcaaatac ttgttcatct ttttcgccgt tcgagaattt
    43201 tttgacgata aatgatgaag tataatctgt gattttcaaa gtcaagatct tgcgttttga
    43261 acgtagttct ctgacttctt tgtcgaatac gtacccctca agtgtgactc ttcgttcttc
    43321 ttcaacgata ctgatcattg gtgtcgttgg ttcatcggct ggaatatttc gtcctagctg
    43381 gatgtggatg ggtccttcaa gagctggcga ttgctctttt ctttcctttt tcttttgttc
    43441 gtggacaact agactttcag ctgcttgctt cataaaggct tctgcctgtt cttgtttgcg
    43501 ttcttcaaaa agtttcaata ctcgttccgc ttgctgctca tccacttctg gttcgatacg
    43561 aaattttggg aatccgtaac tgacgaacaa ctcttctact aatggcaagt attgttgctt
    43621 caaataagaa atagctcctg tactgtcgat tggcaagatg actttacgat cttttatcac
    43681 aggtacctgc gttttcaata cttgctgtgc tagtggtgta tcgcattgct gattttccaa
    43741 agcttgggcc cagtaatctt gcaagagctg ttcatcgaat gtctggtcat ctgctaagat
    43801 attcagtttg acttgtgcaa tatctttaaa agcgatagtc agatgctgag tcaatgaccg
    43861 atatagcata ataggcaaaa ttttggaaaa atgcagcgta aattcccata aacgcgattt
    43921 acgatggacc actacactat ccatttcacc agcagtaagc agtggatgat ttttttctgc
    43981 ttcctctagt tggatttgtt ccaacagttt ttcaaataaa tctcgttttt ctgacaaaga
    44041 tatacgccct ttctgatgag aaacaatgca attgatcgca tcttatcctc ttctttttca
    44101 gtacctttta gtatactcgt tttttctgag ttttacatta aaaaattgga actgcgattc
    44161 aaatcgcagt tccaatttta aaagcgaatc atcagattgt caaaaacagc tgattcagct
    44221 atttttattt tctgtgtttt aacgcttatt attcgccttg gttcaagagg attggtaaag
    44281 tattcaccaa ttcttcttta cgtacttcaa ccatttcgcc agttttcttg attttcactt
    44341 ctacgattcc atccactgct tttttaccga cagtgattct gattggacag ccaatcaaat
    44401 cagaatccgc gaatttcact cctgcgcgtt catttctgtc atcaaccagt acttcatagc
    44461 cagcttttgt catcgcttct tctagttcat tcgtcaggtt tgtttggtat tcgtctttta
    44521 gattcatttg gacgatatgc aaatcaaatg gcgcaattcc ttttggccag ttgattcctt
    44581 gttcatctga atgttgttct acgattgctg ataataaacg acttacaccg ataccataac
    44641 agcccataat cacaaatttt tctcgtccat tttcgtctag tacagttgct cccatagatt
    44701 cactgtaacg agtgccgatt ttgaagatat gtccgatttc gattcctcga gtaaaggcaa
    44761 gaacaccatt gttgtctggt gaaggatctc cttcttgtac aaaacgaaga tcttcataag
    44821 aaagtacagt gaaatcgcgt tctggattca catttttata gtggaatcct gtttcgttcg
    44881 ctccagtaat ggcattcgct aaatcttgga cataacggtc tgcatatact tttacgtcat
    44941 ctgaaacacc gactggtccg acagaaccaa actcagcacc tagatatttt tgtgcttctt
    45001 cttctgttgc ttcttctaaa aagtcagcag ataaaaagtt tttcagtttt acgtcattga
    45061 cttcatggtc tccgcgtacc aaaacaagga caggttgttc atccgcaatg aacaatacag
    45121 acttgatgat tttttgaggt tcaacagaaa agaaatctgc tacttcgtca attgatttta
    45181 cgttaggtgt agctactttc tccaattcaa gctgtgtttc atgcgatttt tttgatacat
    45241 agtagcttgt agccatctcc aagtttgcag cataatcact ttcagtcgaa taacagatcg
    45301 tatcctcccc gatttctgag atcgccatga attcttttga gtctttcccg cccattgcgc
    45361 caccgtctcc gataatcgag cgaaattcta agccacaacg cttgaatact tcggtgtagg
    45421 ctttttcgta atctttatat gtctcgtcca aactttcttc cgttgcatgg aaagaatacg
    45481 catctttcat gataaactct cttccgcgaa gcaaaccgaa gcgaggtcgt ttttcgtcac
    45541 gatatttagg ttggatttga taaagattta aaggtaattt cttatatgaa ttgatttcat
    45601 tacgcatcaa atcagtaaaa gtttcttcgt gagttggtcc aaggatcatt tttcgatcat
    45661 tgcggtcttt gaaacgatac aagtttggtc catatgtttc gtaacgcccg gattcttccc
    45721 aaagttctgc tggcaagatg gcaggcatca gcatttctac cgctccgatt ttttcaaatt
    45781 cttcccgcat aatgcgtttc agtttttcca atacacgatt tgctaatggc agatatgaat
    45841 aaatacccgc tgatacttgg cggatatatc cagctcttag caagatttga tggcttaata
    45901 cttctgcatc atttggaact tcgcgtaatg ttggaatcaa catttttgat tgtctcattc
    45961 tatatactcc tttatttcct caaaatagtt atcgtttgtt attataccct atttgtcaac
    46021 tttttctaga aaaagaatcg ttgaatatcg ttccatgtaa ctagcaccat caacaacatt
    46081 aaaaatccaa agccagctaa tgtcaatatt ccttcttttt cttgacttaa tggtttcccg
    46141 cgaatccctt caaaaatatt caagactaat ttcccgccat ctaaggcagg aatcggaagc
    46201 agattgacga ttccgagatt cattgaaaga agcgccatca aactaataac agttgttaca
    46261 ccttgatttg ctgcttctga agacaattgg tacatcatga caggtccgcc taatttatct
    46321 aaactgaaac ctgtaaatag tgatcctaag gctttgaaaa tttccaatga accgctgaaa
    46381 gcttgacgcg tccctccaat gatcttatcc ataaaacctg tattcattgg ggcttttata
    46441 ccaagttggc caactttttc gccatttgac tctacagatt tcggtgtcac gtcgacagat
    46501 tgcacctgac cttctcgttc gatcttaaag tctaatgttt tatccggatt tttcgtaatg
    46561 actgttgtca gatcattcca tgaatggatt tcttttccgt ccacactgac tacctcatca
    46621 ttttctttta agcctgcttc tgccgcagct ccattaggca tgatctctcc tacgcgatta
    46681 gtgttcgtca cttgaacacc accttgcata aatgccagaa tgatgaataa caggattgcc
    46741 aaaataaagt tattcatggg tccagcaaag ttggtcagca tacgctgcca caatttagcc
    46801 gattggaact gtacgtcttt tggcgcaatt ctgatttctg ttccgtcgtt ttcaataatc
    46861 gtcgcatcgt gatcaacagg gtaggttact acttctgttt cgtctccatt cacataacca
    46921 gtgatagtca gctcatcttc taaatcataa cggctcaatt ccatcgggat agcatttgtc
    46981 aattggattt tcttacttaa atttatcttc tctacgatgc cgtcagaatt tagtaaaaga
    47041 gaaagaggca ttcctggtgc catttctgtt tcatcatccc cattgcctgc cattcgaaca
    47101 taaccgccaa taggtaatag gcgaagcgta tatgttgtcc cgtcttttgc ttgatgccca
    47161 taaattttcg gacccatacc gatagcaaat tcgcgaacga ggattcctga tcgtttcgca
    47221 aagaagaaat gaccaaactc atgaacaatc actaatatac caaaaacgat gataaatgtc
    47281 agaatcgttt tcatagcttt tttctccttt acttgttttt tgtgtttttt ataaaacaac
    47341 gataaaacac tataatttta ccatagtata ccaaagacac caatagtatc aatataatga
    47401 aaaataaaaa cctgcgtctt ttatgaaatt cacaaagaaa cgcaggtata cttactaaat
    47461 aatgccaaat aaatgcatga taggaaatac aaacagcaaa ctgtcgaaac ggtctaagat
    47521 tccaccatgg cctggtaaga tatttcccga gtctttcaca tcataatgac gtttgattgc
    47581 agattcgacc aaatcaccaa attgtccgac tgctgaaaaa ataatcgtca tcagaagcat
    47641 gacaaacata ccatgattaa atagttcttt tgctgggtat agcaataaat aaagaaatgc
    47701 gactacaaca gcactaccaa taccaccgag cgcaccttca atcgttttgt tgggcgaaat
    47761 atcaggactt aacttatgct gaccataacg tcgaccaacc atatatgctc cgatatcagt
    47821 tgcccaaaca ataaatagac caaatatcaa aacagccaag ctttctgttc gtgcattgac
    47881 aaaattctgg aaacctattc ccacatacaa gctgacaagc acaggaaaac ctgcttcatc
    47941 aatcgtatac gtatttttag aaataactga tgttcctaaa agaatcataa ctataagata
    48001 aaataacata aatgtactta cattctctgg aagaaagaaa aaccatcttt cttttggcaa
    48061 aacaagtaac actgcaccaa cggcggataa aactccttca aaacttaaga gtgtcaatcc
    48121 tttcatccga aacagctcgt aaactcctat tactgctaag attgctgctg taagctcaac
    48181 cgctatgcct ccgatccata taatcggaat aaacaagact agcgccacgg ctgctgtgac
    48241 cacacgttgt ttcatgactg atcccctttc tcatcttctg ttttttttac accgccgaat
    48301 cgacggtctc gattttgata agaggcgatt gcttcttcaa gatgcgcacc atcaaaatca
    48361 ggccaaagcg cttttgtaaa atacagctca ctatatgcga tctgccataa cagaaaatta
    48421 ctaattcgct cttctccact tgtacggatc atcagctcag gatcttgtaa ctctttaggc
    48481 aagaaacctg tcattaaatg cttagcaatc aattcatcat cgatttcttc tgcgtgtatt
    48541 tctccttttg ctacttcttc ggcaatttct tggacagcag tcacgatctc tgctctgctt
    48601 ccataattca aagcaaaatt caataccatt ccagtattgt tttttgtttg ctcaattgca
    48661 cgtcttacag catcttgcgt atgttctggc aatacgtttt cataacccat gacatgaact
    48721 ttgacatttt ctttaatcaa ttctggtacg aacgtatcaa agaagtcgac aggtagttgc
    48781 atgagaaaac ttacttcatc ttttggtcgc ttccagtttt cagtcgaaaa ggcataaagc
    48841 gtcaacactt tcactccaag ccttgatgct ttttttgtga ctttctttac tgtttccatg
    48901 ccttctttat ggccagcaac tcgtggcaaa cgacggtttt gcgcccaacg accattgccg
    48961 tccatgatga tggcaatgtg ctgaggaatt ggtccatcgg tattaaattg aaattcgctt
    49021 ttttcttgaa tatatttgtt tttttgcgga aaaaaacgta acattctttt tcctcctata
    49081 cttaaaagat tcccactatc ttaagtatta tagcaatatc taactgtttt tcctatgaaa
    49141 tcattgtttt tttatttaaa aactgaataa ttttttcttt ttttaatttt taaataaaag
    49201 aaatataaat aaaaacagag gaagcggaat gatccgcccc tctgttttta tgattttatt
    49261 aaacttccaa taattctttt tctttatcag cagcgatctt atctacttct ttgatgctgt
    49321 tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa atcatcttcg gtaatatcac
    49381 catttttttg ttgtttttta tattcatcga tcgcatcacg acggatgtta cgaatcgcga
    49441 tttttgcatt ttctgcttct tttttaacat ctttagctaa ttcttttctg cgttcttctg
    49501 tcagttgcgg gataactaag cgaatgacat ttccatcatt ggttggactg attccgatat
    49561 cacttgccat aatagctttt tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca
    49621 ttaaaacacg tgcttctgga atttggattg atgccatttg gttcaatggt gttggcgcac
    49681 cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt
    49741 gcccaagttc acgttgaaga ttttgtgctg cttttttcat tttttctttt gcttctgtca
    49801 taattgcatc tgccattttt atttccccct tacagttgtt ccgatatttt cgcctaagat
    49861 tgcacggcga atatttccgt gttcatttaa attgaagaca agcagtggaa tatcattatc
    49921 catgcttagt gaacttgcag ttgaatccat gacttgtaat cctttggcaa taacttccag
    49981 atgagtcagt tcttcaaatt ttactgcatt tgcatctact ttcggatcag cagaataaac
    50041 accgtctaca ttgtttttag ccatcaggat aacatctgcg ccgatttctg ccgcgcgtaa
    50101 tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta ccgcctgcaa agataacgat
    50161 ccgtcctttt tctaaatgac gttctgcttt acggcgaata tatggctctg cgatttgacg
    50221 catttcaatc gaagtttgca cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa
    50281 agctaaggca ttcataactg tagctaacat acccatgtaa tcagcttgag cacgttccat
    50341 gcccatttgt gctccgattt gtccgcgcca gatatttccg ccgcccacaa cgattgccat
    50401 ttcaacgccc agctcgtgaa cttctttgat ttcttggatg atttctttga ttactggtgg
    50461 tttgattcca aatcctgcgt ccccagctaa agcttctcca ctcaatttta agactacacg
    50521 ctgatatttc ggtgtgacca tgttcattcc tccaatattt tctattgcca ttttaccata
    50581 cttagatgat ttcgacactc taagactggt ttttcttgtt ttgcttaaaa aaagggagcg
    50641 cacaaaccgt gcgttcccgt ctgttattca acagaattat tttttcactt ggctcataac
    50701 ttcttctaca aagttatctt cgcgtttttc gataccttcg ccaacttcga aacgtacgaa
    50761 tgttttaaca gttgcgcctt ttgatgcaac gtatttttca acagtcatat caggatcttt
    50821 aacgaatggt tggtccacca aagcgatttc tgctttgaat ttcttcaagc gtccttctac
    50881 cattttttca acgatattcg ctggtttgcc ttcgttcaat gcttgttctg taagaacagt
    50941 tttttcatgt tctaattcag cttctggaat ttgtgattcg tttacataac gaggattgat
    51001 agctgctacg tgcatagcta cgtctcttgc aactgtttcg tcagttgttc catctagaac
    51061 agtcaatacg gcaatacgtc cgcccatatg caagtatcca ccaaatgcag cattgtcgtc
    51121 tttttctaca acttcaaagc gacggaagct gattttttct ccgataactt gagttgcttc
    51181 gatcaagtca gattcaattg tacctttatc tgttttgatt ttcattgcag cttccatgtc
    51241 tgctggtttg ttttcagcaa caagttctgc aatttctttt actaaatctt ggaacatttc
    51301 gtttttagaa acaaagtctg tttctgagtt tacttcaacg attgctgcag tgttaccttt
    51361 cacagcaact gaagccaatc cttcagctgc aatgcggtca tttttcttcg cagcctttgc
    51421 cattcctttt tcacgtaaaa gatcaactgc tttttccatg tcgccttcta cttcaactaa
    51481 tgcttttttc gcatccatca ttcctacgcc agtcatttcg cgtagttctt ttaccatttt
    51541 agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc
    51601 ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca gctccatcat tcaaaattat
    51661 tctgctgaag aattgtcgcc ttcaacaaca tctacgattt cttcgattga tgttgctgtg
    51721 ttttcttctg tgaaatcttc ttcaactact tgatcttcac cttggtttcc ttcgatgaaa
    51781 gcatcagcca tttttgaagt aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg
    51841 attactacgt cgatttcatc tggatcgcag tttgtatcaa ccatagctac gattgggata
    51901 tttaatttgt gtgcttcttg aacagcaata cgttctttac gtgggtcaac gatgtacatt
    51961 acatctggga ttcttggcat atcagcgata ccgcctaaga atttttcaag acgttcacgt
    52021 tctttgttca aaccagcaac ttctttttta ggtagaactt caaaagttcc atcttcttcc
    52081 attgcattga tttgtttcaa acggctgata cgtttttgga tagtatccca gtttgtcaat
    52141 gttccgccta accaacgatg gttaacgaag tattgtcctg cacgagttgc ttcttctttg
    52201 attgcttctt gtgcttgttt ttttgtacct acgaataatg ctacgccgcc ttcttcagca
    52261 acgctcttca tgtaatcata agctgcatct actaatttaa ctgttttttg caagtcaatg
    52321 atgtagattc cgtttctttc tgtgaagata tatttcttca tttttggatt ccagcgacga
    52381 gtttggtgac caaagtgtac gccggcttct agtaattgtt tcatagaaat tactgccatg
    52441 tttgtttcct ccgatttggt tttatttttc ctcttcttat cttctacctt gccggcaaac
    52501 tttttcaagc acctaccgac aatccaataa gaatgtggat ttgctgatgc ataaagcacc
    52561 ctcagctatt atacagacaa aagatccgta tcgcaagagc aaatatactt ttctgctcaa
    52621 cattcaatag tttacctgaa agataaaaag aattcaagaa aaacagtaga tttctctttg
    52681 tttctttatt atcctttttc cataaaataa ttttaggtat aaaagacgaa tattattggg
    52741 caatccttct tttttttgtt atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg
    52801 cgcaaagctc aaagacattt gctgattaaa caagtcattg aagaattccc gatccgtacg
    52861 caagaagaac tattaaacaa acttgggcat tatggcgtta ccgccacgca ggcaacgatt
    52921 tctcgagata tccgtgatct aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc
    52981 ttcgtattgt ttcgcgaaaa aggaactcct gagactagga ctgaggaaga ggatcggctg
    53041 gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc
    53101 aataccttgc cagacaatgc tcatttattt gcatctgttt tagatgagat caaacctccc
    53161 tctattgtca gtacaattgc tggattcgat acgatcatgg tcatctcgga aaatgaggag
    53221 gacgcaaaac gagtagaaga atttcttcac catccaactg aagtatcact tttttaatca
    53281 gagcacgtat tcctaatatg gaatgcgtgt ttttttattt tcaatatttt ccttattgaa
    53341 taaaccttta cactctttac aaaaatacat cattttttat tcaaattttt caacaccttg
    53401 attgaatcac aataatcatc atttatattg gatgttaaca agagcagctt cttgctcttt
    53461 gtgtatttca aaaaagaaat gaaaggaaat catatgtata aattattaac ttctggacca
    53521 ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg atcattgtac ttgggatcaa
    53581 gaatacaaag aaattacaca atggataaga aaaaaattat tggatcttgc tcaagtatcg
    53641 gaaggaatat acacaacggt cttaatgcaa ggtagcagct ccttcggggt agaatcggta
    53701 ttaagttctg tgatcaggcc agaagatact gtactgattt gttcaaacgg cgcatataga
    53761 caacgaatga tcaacatgtg cgaatgtctg aaactgaact ataccgttta tgaggtatcg
    53821 gaacatcaaa tcctccaagc tcatgtgatt acacagctga ttgaagagaa tccgtctatc
    53881 tccgcactag caatggtcca tagcgagact acttccggta ttttgaataa tttggaagag
    53941 attgctgctg tcacacaaga aagagatctt ttgtttatag tggacgcgat gtcaagcttt
    54001 ggtgggatac cgatttgtgt agccgaacta gggattgatt ttttgatttc cagctcaaac
    54061 aaatgtattc aaggtgttcc aggcttttct tttgtacttg ctaataaagc ggaactggaa
    54121 aaacgaaaaa atgttagccg aagtttgtca cttgatctat atgaccagta ccgcgtgatg
    54181 gaaaacgaga atggcaaatg gcgtttcact tcccctaccc atacggtttt ggcttttcaa
    54241 caagcgctga aggaactaga ggcagaagga ggaatcgaaa ctcgatattc acgttatctc
    54301 gccaataatc atgtgctacg acaaaaaatg acagagatgg ggttccagcc tttcattcaa
    54361 gaatatcagg ggccattcat cacctctttt ctttacccta aggactggcg atttgatttt
    54421 aatcattttt atcagtattt aaagcaaaat ggatttgtga tctatcccgg gaaaatcagc
    54481 gatcatgaat gttttcgtat cggaaatatt ggcgagatct atccggaaga tattttggaa
    54541 ctgacagcaa ttatcaaaga ctataaggag aacgttaatg attaaaactg taatttttga
    54601 ttgggcagga acaactgtgg actttggctg tatggctcct gtccacgctt ttagaaatgc
    54661 ttttctagaa aaaggaatcc aactgactga caaagaaatc cgctaaccga tgggaaaatt
    54721 aaaatgggat catatccaac agcttctttc cctcccttct gtcaaaaaac aatggatagc
    54781 tatttatgga caactacctc aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca
    54841 ttatctgttt aaagatttgg tcaagcattc taccttgaaa cccgacacat tggaaacgat
    54901 cgaacggttg agaaacgctg ggatcaatat tggttccacg aatggattta atgctcctat
    54961 gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat tcaccagact tcatggcaac
    55021 ttttgaagac gtcgaagggt atggtcgacc gtttccttat atgatccata aaaacatgca
    55081 gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg tctcagatat
    55141 ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta attgaaggta gttcgctaat
    55201 ggggatttct tatgatgagt accaaaaatt agagatgggg aaaaagatcg tttaaaacag
    55261 tctgttgctc ttagattcca acaagccggc gctgatattt gtgtaaacaa tttaaaagag
    55321 ttaacagaat acattttgta cgctgacatg aatccagaat gacttttata caaaataaga
    55381 ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag ccaagcaaaa atagctcctc
    55441 atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttattc
    55501 gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
    55561 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
    55621 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
    55681 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
    55741 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
    55801 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
    55861 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
    55921 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
    55981 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
    56041 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
    56101 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
    56161 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
    56221 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
    56281 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
    56341 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
    56401 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
    56461 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
    56521 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
    56581 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
    56641 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
    56701 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
    56761 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
    56821 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
    56881 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
    56941 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
    57001 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
    57061 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
    57121 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
    57181 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
    57241 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
    57301 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
    57361 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
    57421 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
    57481 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
    57541 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
    57601 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
    57661 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
    57721 ctagtcgttt cataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat
    57781 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
    57841 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
    57901 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
    57961 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
    58021 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
    58081 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
    58141 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
    58201 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
    58261 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
    58321 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
    58381 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
    58441 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
    58501 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
    58561 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
    58621 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
    58681 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
    58741 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
    58801 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
    58861 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
    58921 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
    58981 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
    59041 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    59101 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    59161 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    59221 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    59281 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    59341 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    59401 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    59461 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    59521 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    59581 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    59641 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    59701 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    59761 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    59821 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    59881 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    59941 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    60001 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    60061 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    60121 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    60181 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    60241 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    60301 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    60361 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    60421 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    60481 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    60541 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    60601 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    60661 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    60721 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    60781 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    60841 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    60901 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    60961 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    61021 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    61081 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    61141 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    61201 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    61261 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    61321 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    61381 ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa
    61441 attaaaaaaa taaattatat tatcgttgtt agcgtttact atatgtatta ttgttgtttt
    61501 ttatacacaa atcattcgct tcaaaacaaa ttattcaata ccattgtata ttttttacat
    61561 aataacaaat tatatttgta aagtattaaa acttgtttct aatgttggta tgttaaaact
    61621 ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa caataaggag aggacttttt
    61681 aatgcgtgtt aataaaatcg attttcactt ttcaaaactc agatatcaac ctgattttga
    61741 acatttctcg gatgaagagt tccaacagat caaggaacat actgttcttc gttcttataa
    61801 aaaaggacag atccttttcg acgatggtga tcccagacaa cggttgtata tcttgaccaa
    61861 aggactcgtt cgtttagaac gttatgatga aagtgcaact tattactact atgactatgt
    61921 gaatacaaat aacctttttc ctatgtcagg aatgtttgga gaagagacct acacttatac
    61981 agctcaagcg atgactgata ttgaaacatt ctaccttcct gtttcctact atgaaaaatt
    62041 agcacgaaaa aatcccgaac agttgatcta tatgatacaa aaaatttcta aagtagttga
    62101 aatacatgaa ttgcgtatcc aaatcggatt gacctctagc gcctttgacc gggtaaaaca
    62161 aaatttattc attttgaaag aagaattggg cgtaccaaca aaagatggtc atatcaccat
    62221 tccttatccg atcaccttaa aagaattagc agtgaatagc ggcacgacac gtgaaactgc
    62281 tggacaagta atcaaacaac tacgtgattc cgggctgtta gattatgaca aaaagcgttt
    62341 tactttctgt aagacagcta ctactccagc tatgagagaa taacgcgaac aaaaataagc
    62401 agtgaccctg ataaatatca gggtcactgc ttattttcct ccctaaaact tctgaaagat
    62461 ccggcagttt tagatcactg gacaagactc aaagattttc tatgtgtgtt tcagttatgt
    62521 aatattcact gtttgcaaaa aatactgtta gattaactag atgaagcgtc ttgtcctcat
    62581 ccgatctaga ttattcgata ttcctcgaca ataaacgatt actcagtgag ggcaatcaga
    62641 ggaacggata atgataaata agctgtaaaa aatagcaaaa taacaataat gattaaaagt
    62701 atgtaaaaaa tagggctttg acaaaaacgt gtcacagccc tatttcgatt aaacggtgtg
    62761 tacctgtcaa ctcttcggtc acaagtcata atgttcataa acatgaggag ctgttttttg
    62821 aaattgttcc ttgtgaatca gagttaaacg acaggcttac gaactcttga ttaaacggcg
    62881 ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc aaaaacatga gaagccgttt
    62941 ttcctgggct gttcttatta cttgatgcag ggcagctttt tcacaacctc tttattcaga
    63001 taaaaccaag cttggatcag cattcaactg atagctagca aaatttcttt tttgcatttc
    63061 cacatgtgct gctgctccaa tcattgcagc attatctcca caaagtcgaa gagggggaat
    63121 cactagttct acctcaggga gtttagctgc caaagcatcc tgcaatcctt cacgcaaccc
    63181 ttgattggcc gctacacctc cagctataat cagttgcttg actggataat tttgacaagc
    63241 tcgaagtgtt ttagtaatca atacatcgat cacacttgct tgaaagctag ctgctaaatc
    63301 atttttgtct agatcttctc cacgctgttg tgcattatgc accaaattga taaatgcact
    63361 ttttaatccg ctaaaactga aatcataatt atcttcatgg atcatcgctc gcggaaaatg
    63421 gtaattatct tttccctgat gagctagctg atcgatttct tttccgcttg ggtaagaaag
    63481 acctaatact cttccaactt tgtcataagc ttctcctgct gcgtcatctc ttgtctcccc
    63541 aatgatttca tatgatccat cttcttgcat atagactaat tctgtatgtc ctccgctaac
    63601 aagaagtgcc atcaatggaa actggaatgg tttaaccaga cgggctgcat agatatgccc
    63661 tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc caagcaaaag ccttagctgc
    63721 agaaatacca atcagcaacg agccgacaag tcctggtcca taagtgacag cgactgccga
    63781 taaatcttct gccgacacac cagcttctac cagtgcatct tccaagcata aggtgatttg
    63841 ttctacgtga tggcggctag ctacttcagg tactacgcca ccaaaacgtt tatgactgtt
    63901 tatttgagaa gcaacgatgt tagataagat ctctgttcca tttctaacga ccgctacact
    63961 cgtttcatca caacttgatt caatcgccag aatcaattct tcattcattt catcgtctcc
    64021 gtttttaact ttctgctcat aatgatcgca tcttccgtcg gattgcgata atatgatttc
    64081 cgtttaccta atatacgaaa cttttctgat tcataaagtt ttcttgctgc ttcattagat
    64141 agacgtactt ctaaaaaaac atgcagcgaa tcttttattt gttccgtaat cagtaattgt
    64201 aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg caacagcaat atttgtgatc
    64261 tcgacttcat ctattacttt cgagtatcca ataaagccac agatctggtt atcttcagct
    64321 aaaagcagat aatcagagtg aggctgttgg atgtctgcca aaaactgcat tttcgtccaa
    64381 ggtgaaccat gtggataagc attttcgcta atcatccata cgcattctgc caagtacgct
    64441 tcatcaaaat ctttttttct gcaaatcaac taatctcact cattttctaa aagttttatc
    64501 atatccaacg catcttcttt tgtttcagtg taatatcttt ttttgatcgt tgttgcttga
    64561 aaccccaatt tccgataaag tctttgtgca tcttgattac tcatacgcac ttctaatgac
    64621 atcgtctcac agcgattcat tatagcaaag ttctcgattt cgtcaataag cagcgaaccg
    64681 attcgtttac gctgatatgc cggtcgaacg gcaatattcg tgatatggca atctaaaccg
    64741 aacgctcttg aaccgataaa accaatcatt cttccattat caaagatacc tagatataga
    64801 tgaggaagcg gtgacttcaa ttctgatata aaggcacttt ttgtccatgg caattcgcca
    64861 ttatagactt ctcgttcgac atccagcagc tctttgatat cttctgtagt caattctctt
    64921 agtacgtaac gacgatcgat ttgttcgact gttttttctg tataaatcat tttatcttga
    64981 aaaaagcttc tggcatacgc tctaaatttt ttcaacataa ctttcgtctc caggtgtgtg
    65041 cgtctccaac catttttctt ctgcttctac tcgttttaaa tagcgaggaa gaaaagcttg
    65101 tttctctgca actggttcaa ctaaagcacc taattgagcc acagttacac cacttggaat
    65161 atcccatgct ggaactgtat taatcatcgc actaggcaat atttccttta tttcagagaa
    65221 gaatttgtga caatcactcc cgacgaaata aatagcttca ttcagctttt gtagttgtgt
    65281 gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca gtttctaatc tattttcttt
    65341 ccagcggtat gccccagcgt atacattttt tcttcgagca tcaaacatgg gcacaataat
    65401 attatccaca ccgatgcaat tagccgcgat ggttttcaga ctagatactc ctactagctc
    65461 tttatctaat gtataagcaa tcgtttttgc tgtcgttaca cctatacgaa gccctgtata
    65521 agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct tttggactta tttgaagatc
    65581 tgcaaacaat tggtcgattg caggcatcag tgttacactg tggttgcgtt tgatatttgt
    65641 ttgtatttgt ccaagaattt gttcgtcttc taccacaccg atcgccaaag tttggtttgc
    65701 agtatctatt cctaatgtaa tcatatattt gatcctttct gctatgacat tctcgccttc
    65761 cattgtagca tactttttta ttctgaggat gaaaatacca aaggcaaaac tagtacaata
    65821 atacatgatt atctgctggc gggcaaattt tgccccttcc tgaaacacaa aaaagctgcg
    65881 gtatcactcc attttcccac agcttttcca tcttttattt cgttgattct cgaataatca
    65941 actctgtttc cacaataatc tttcttgaaa ccggtgcttt ggaattactt aattgaagaa
    66001 tcgtctccac tgctagctct cccatccatt cggtataaac ttttacagta gaaagcgagg
    66061 gcgttacata tttcgctaca ctgacatcat tgaagcccat cacagaaata tcttctggta
    66121 ccactaaacc tttttcttga atcgctttca gtgcgccaat cgctaatgca tcattagctg
    66181 caaaaagcgc atcaggaaca tgcggattcg tttttaaata ttgcttcact gcttgatatc
    66241 ctgactcgac agaaaaatct gcttcgataa ttggaccttc gtcgtagcct ttttctgcca
    66301 aaacttgttg aaatgccata attctgggat cttttagccg ctgatggttt ttcttcgtat
    66361 attctgttcc agcaagcata gcgatatgtt gcgctttatg ggagacaaag tgttcaatga
    66421 ctttttgaac acttgactta aagtcgacaa ctagagagtc gattccttga gaagtgccgt
    66481 cagaatctac caaaagcaag gcattttggg atcctttcat ttctaataac tgagcttcgt
    66541 cgaattttcc tagtgcgatc gttccatctg ccggaagatc actaatggaa tcccacgttt
    66601 ctttcaataa agtaacgttt gcttcttctg cctttttttc gattcctagg cgaatagata
    66661 aataataaag atcttcaagc tcttccgtat cgtcgtacca ctggatcagg cgaagcgttt
    66721 gcttagtatt tgctgcattt ttacgatgtt tcgtataatt taagcgttct gctgcttcaa
    66781 aaattttttg ctttgtggct aagccaacgg aaagatcagg atcatagttg agaactcttg
    66841 aaacagtagc aggagaaact cctgccaact gcgcaatgtc tttaattgtt gccatttgtc
    66901 tctcctcctt ttttacgttt ataaaatcgc agtaaatcgt tcaaacgctg cctgtccttt
    66961 ttcatctcgc ttgaatactc cagcgtcttc cagtacacgt gcaaaaacct gacctacaga
    67021 tttttgtacg acatcggtga ccgtatcttt cgtcaaatca ggatgttctt tttttaattg
    67081 ttctgcccac ggacgatgat attcagccat ctggttttct tctcctaata agaaatgctc
    67141 tacttctact agttctggtt tcaaacgagg tggtaaaaca gcaagcccca ttacttcgat
    67201 caacccaatg ttctcctttt tgatatgttg gacatcttta tgtggatgaa aaatcccatc
    67261 aggatgttct tctgaaacat tgttgtctcg aaggacaagg tccaactcaa agtaattgtc
    67321 ccttcttcgt gcaattggcg taatcgtatg gtgtggtgta ccatctgctg aaaaagctct
    67381 gatagatact gtttcatccg aatatgcttt ccatttttcc aatactgagt tagctgcttc
    67441 aataagcaag tttttctctg ttgattgcaa acggatcaca gacatcggcc atttcacgac
    67501 tcctgcgatg acttgcggat aatcttctag tttgataagt ttttctatcg gtgcttttgc
    67561 cataggaaat tcatgacgtc ctgcctgata atgatcatga gaaagaatcg atcctccaac
    67621 aattggtaag tcagcattcg aaccaacaaa gtaatgaggc aagacttcag taatccgaag
    67681 cagtctagca aatgtttcct ttgtgatttt catcgggcgg tgttgttctg ataaaatgat
    67741 cgaatgttca ttgtaataag cataagggga atattggaat ccccagcttt caccgtctag
    67801 attcattcga ataatccggt gattggtgcg ggctggatag ttcaagcgac ctctatatcc
    67861 ttcgttttcc atgcaaagca tgcaggtagg atagtctact tttaccgcct cgcgttctgc
    67921 tgcgatttgt tttggatctt tttctggctt agataaattg attgtgattt ccagttcacc
    67981 atactccgac tctgccggaa atttgatgtt ttttgcgatg gctcttgttt tgatgtagtc
    68041 atttttctta cttaaattaa agaaatactc agttgcttcc tctgggcttt tcgaatagtg
    68101 ctgtgcaaag aatgcattga cgactgaagg tggcggtgtg agaaaatcca ttaattgcgc
    68161 ttcaaaaatc tcttgttctg ctggcaattc tgaaagaaca ccattttctc ttgccttagc
    68221 gacaagctga tccaataagt ctacagattc ttccttaact ggtcttactt caatgctttc
    68281 tagagcatct tctcctacca tccctaaaag acgattctgt aaatataagc ggtccatttc
    68341 catccatccg ccagcttcga tcgcaagtgt ggcaaaatca caaatcgttt gactgattga
    68401 catatctcat tcccctattc ttcataacca tttggatggc tgacatgcca atcccatgct
    68461 gttttgataa tcgctttgat atctgtgtat tcaggtttcc agcccagaat ctctctcgct
    68521 ttttcacttg aagcgactaa tcggctagga tctccagctc ttcttggtgc tatttctgct
    68581 ggaatttctt ttcctgtcac ttctctagct gcttctagca tttccttgac tgagtatcct
    68641 ttgctgctgc ctaaattgaa gaagttgctt tcatttcctt ctttcaagta ttccaaagct
    68701 aaaatatgtg cagcaatcaa atcttctact tgaacataat ctctgataca tgttccatct
    68761 ggtgtatcat aatcgtctcc ataaacagcc aaggcttttc tttgaccaag agctacttgt
    68821 aaaatgattg ggacaagatg cgtttctggt gtatgatctt ctccgataga tgcatctgct
    68881 tttgctccgg ctacattgaa gtagcgcaaa gcgacatagc gcatgccata tgcttggtca
    68941 caccacttca tcattttttc catcatcaat ttactttcac catacggatt tttaggatta
    69001 gctggtgtat cttctgtgat tggagactct ttcggctctc cataagttgc tgcagtagaa
    69061 gagaatacga ttttattcac gttgaactcg tgcatgactt caagtaagat ttgcataccg
    69121 tatacgttgt tgttgaaata catcaatggt ttttcgacag attctcctac taaagagctt
    69181 gcagcaaaat ggattactcc ttcaatcggt tccttttcaa atacggaacg tagaaattct
    69241 ttatcccgga tgtctccttc ataaaaatga gcatctggat gtactgcttg tttgtgacct
    69301 gtgagcaaat tatctaccac agctacttga tacccctttt gaactagctg gtctacagcg
    69361 tgcgaaccga tataaccggc tccgcctagt actaaaattg tcataaaaaa cactcctttg
    69421 attagtaaaa ttgtgtttgt tgattctata taatttaaaa aacgaagttt acttttatgg
    69481 tgaaaacaaa caaagtaaaa tttcgtaaat tcatatggta aatttataat aaaacattta
    69541 gtaaacattt tcaagggttt tttgcaaagg tttacaaaca aacgtaaaac tagccttttt
    69601 ttctcttttg ttccataata gatataacaa ataataaatg aggtggattc aatgaaaaaa
    69661 tttgtatctg gaatattagt aggaagtctg gcaacagccg ctgccgttgc tggtttggtt
    69721 gcttctgtga aaaaaacagt gatcgatcca attgacgaaa aagaagcaat gatcgaagaa
    69781 aaccgtaaaa aagcaatgcg taaacgcgtt tctcgttaat aattatttaa aaatacgcat
    69841 gaagactttc taaaaaacag ataagtcatc atgcgtattt ttatttgtca gtcaatataa
    69901 gttgtgctat tttatctgcc tgtctcagat tccatggatc tgtataaggt ctagctactg
    69961 ctgcagttag ccaatccaca ccttcaacag gtattccata gcaataaaag tcagactgga
    70021 tttcccctga agcatcaatc acgcgattcg tacctcgctc aacatctata gcttctgaat
    70081 gataacttcc tgtattatct gaaaatgaaa aagggcgaac aagtttgttt tccttcaaat
    70141 tttttatgac aggattactt gaacgagaaa agcttgtttg tggcaatcga gcttcaatca
    70201 aatagcgact gctgatttct ttatcttgta gagaaataac atagcggttg tgcttttgct
    70261 gaatatcaaa tggcggttcg atcaaatgaa aaatacccgc atctatcagt gctgctaatt
    70321 ctttcgtccg aatgattggc ggaccgatcg ttaaaaagct atttagatga gtaaaccatc
    70381 cccatagtat ttctttgtat tccttagcag aaaaaacgtg ccattcaata gcctgccgga
    70441 ttggatcacg ccagtctttt aatgcatcaa atgttgaagc aaatgctcct gtgcaattgc
    70501 ctttttccgc ttcttttatc tgcccttcaa taaatattcg gctagcttct gaaaagggct
    70561 cattctgata ggcaagagga gtttctaaat acgaccaatc ccatctataa gggattagtg
    70621 ctggatgctt tccgagacac tcctctgaag agagggagag aaaatcatgc tcaaaatttt
    70681 tctttggaat cggcaagtga tattcttcga tgattttctt gtaataaaac aactcaacgt
    70741 cttttttcag atagtcaaaa aatgtttcgc cagtcaaaaa tcccgactga tgccattgct
    70801 ccaggttttc ttttgttagt aaccgtggcc atgccattgc tccgccttgc ttttgatttc
    70861 ttcctcgagg gtaataaggg agcccccttc ttgatccact gtatacaatc ggttccttgc
    70921 ctgatggatg atagaccagt tttccctttt cgttctcgaa gcgccctcct cttgatactg
    70981 taaacaatcc gacataatca aaaaaactta agcctagacc tcttaaaaag acattttctt
    71041 taggcggtat cttcgaaaga tctacatctg caggattaga aggaagctga tacagtaaaa
    71101 catttttctt tgcgtagtct acccacactc tttcttcttc tgtctcatca ttttcccagt
    71161 gaccgcttgc taaaacaagc tgatcgatag aatagctttg ctgatctgtc tgtaaaagat
    71221 aaccatcttt tcttttgctt acttttttta ctaaagaata aacaaactga atagaagctg
    71281 gaaattcttc ttgcaacttt gagaaaaacc agcgttgata gagtccgtat aaacatcgag
    71341 tagattgctg atctttttcc agacgattga tttcttctat aattagttgc ttatttttgg
    71401 ttaccgtctg ttcgatataa tctgtagcag gtcctttact ccattgaaac aagttgggtc
    71461 cctgtgttac caatcctcca ctggacaatg tttcatctgt aaaaagcgtc acttgttgag
    71521 aaacagaatt cattaataac tccggtggct gattcaaccg ccaaactcga cctccaggac
    71581 cattgggatc gaaaagaaaa agttcaagag aatgtctgtc ctgattatta tggagaatcc
    71641 tttctgctac acttaatcct ctaggtccgg caccaatgat ccctattttc atttgcatgc
    71701 ctccatttcg ataaattttt tcaataaaac agcatggttc tgatgttcat tttttgcacc
    71761 ataaacaaaa gtaatgactg gatgttcttt cataagttga ctgagtttaa aaatagcttg
    71821 cttcttttct tcttcgaaaa tcaattcttg ctggtatttc tttgcaaatg aagcaaatcg
    71881 ttctggtaca tggtcaaaag ttttgcgtgc ttcattacta ggtgcaattt ctttcaacca
    71941 cagatctaac tgcgcttttt cttttgaaat tcctcgtggc cagaatctat ccacgagtat
    72001 tctatagcca tcctcttttt cagcatcaag gtaaacacgt tttaatttga tcatagttgt
    72061 ttccttcttt ctaccttttc gcttagctta tatatgtaga taatagcaca aaataaaaaa
    72121 aatgaacggc acaactatcc attgcgccgt tcatgtgatc ttggtcaaaa tctaatgatt
    72181 gattcggttt ttaaagctgt catacaatca tctgttttat acttttttac ttttgatttt
    72241 tccagaccat ccgtcatatc cacccttaag taaataaatc tcttgataac ctgctttacg
    72301 taatttattt gctgtacgga tgctcaaact tttcttttga tcgtaaatat agacaggctg
    72361 gtcttttcga atagatccta atgaatcttt caaaacagtg taagggatgt ttcttgcacc
    72421 taaaatatgc ccagcatcaa acacatcttt ttcgcgaaca tcgattactt gtgcttttct
    72481 cattccttct cgaaattctt cttctgtcaa agtcgttgct gaacgtttgg ccattactcg
    72541 tagatagact tcccaaagga tgattgcaag cacaatcaat agcaaaatga tattaattac
    72601 ccataaaacc atgtaatgcc tccgtttttt taagcaaatt tcaaggctaa tgaggcagca
    72661 ccaatgacac cagcttcatt accaagctct gctaatttga tttttgttga ttctcttact
    72721 tgcgggaaag taaattcttt aaaatatttc tctacacggc tacgtaaaaa ttctcctgct
    72781 gccgatactc ctccacctaa cacgatactt gacggattta atgtattccc caagtttccg
    72841 caagctaaac ctaagtagaa gcatacgcga tcaacaacca tcaatgcaaa cggatcgtct
    72901 tcttgagcca tttcaaagat atctttactt gttacttctt ctccgttgtc tagcattgct
    72961 ttcagttttg aatcccctgc gtattcttct gccaagaaac gacctaggcg taccacacct
    73021 gtagcgctag atacagtttc taaacatccg cgtttaccgc aagtacattc gaacccttca
    73081 ggatccacag tgatatgtcc gatttctcct ccagctccgc tcacgccgtg aatcaaattt
    73141 ccgccagcaa cgatacctcc gccaacacct gttcctagag taacgaatac aacgtctgga
    73201 tcgttttctc cagcaccttt ccatcgttca cctaaagcag caacattcgc gtcattatcg
    73261 atagcgaatt tgattcctgt tcctgcctcg atcagttttt tcacaaattg aacttctgtc
    73321 cagtttaagt tatacgcacc aataaccgtt ccagcttcgc tgtccacgct tcctggggta
    73381 cccatgccga ttccgataaa gtcttcggct ttcatattat ataaattcaa acgatgattg
    73441 attgattcaa tgatttcagg aacgatatgc ttaccatcat ccaaaatatt ggtatcgatg
    73501 ctccattttt gctgaatctc tccttctggt gttaaaattg caaattttgc tgttgttcca
    73561 cctaaatcaa tcccaataat cttcttgtcc attacaaatt tcctttcttt cggctttctt
    73621 caattcggtg ctctcttttt aatatgcgcc acgctgtcat gtatgtatca tgatcaatca
    73681 aacggctatc atacagatta cgcaattcga tagtcattaa ttcaatgtca tagatgcgtg
    73741 cacccacata gataaagatg ccaaattttt tgaacagctg ctgtacatca tataagctct
    73801 ccatctttaa gcacttcctt catattatac aagtaaacca tattttttta aacccagtaa
    73861 aatacatatt acaaaaagaa atacaaaaac tagtgtagaa agaatccttt ggtgtatcga
    73921 atagcttgtc tgttgtttcg gtagacccaa aacattccct aacactaatc cgccaattag
    73981 cccaccgaca tgtccccaaa catctacaga ggtatccaga agaccaaaaa caaatgtcaa
    74041 cacaataaat aaaagaaaat gtctgactag ctgctggatc gctgggttat gtttaaaatg
    74101 aaaccctaag ataaacaacg caccgaacaa accgaatatc gaagtacttg caccaccaga
    74161 cagaacattc gcttcgttaa aagcaaagct tgctgtattt cccattatcc cgctgaacat
    74221 ataaatcaag aaaaaacgcc aatggccata gattgcctcg atttgctgcc ccataaaata
    74281 cagtatcagt gagttcatta cgaaatgcat cagtccaaaa tggatgaaga ttggtgtcac
    74341 taaacgccac cattcattga agtgaacaag ataaggacca tacattccgg cataataagg
    74401 gatctcaagt cgcggaaata attccatcat gaggaagacc gctgtctgta tccctaaaaa
    74461 agcataggtc cataacggtc gtcttaacca tgcacgcaat tttatttgct gttgataatt
    74521 catgaaaacc tcccatttca ttttagtcaa taaacaattg ttggatcttc tgatcaaagc
    74581 tctccggttg ccattgttca ttcaattgtt cacgaaaaac aaggctacat gtttttttat
    74641 gaaaattatt taaataccgg tcgtaataac caccgccaaa accgattcga taaccggctc
    74701 tattgtagat aatacctggt acaatgagga gatcgatcat ctccggcaaa tagagcttct
    74761 ctgattgtgg ctcaagaaca ccaaaactcg aacgtacaag aacggactgt tcatccattt
    74821 cataaaacgc catctgacgt ttcggcaaag ttttaggtac aactattttc ttttgttctt
    74881 tttctgctcg ttggataacc ggctgtgtat ccagttcaaa ttcattcgat aaagtcgtcc
    74941 ctatcacttt tgctgacttc cagtactcgc tggcgaatag ctgtttatag attcgctgtt
    75001 ctttttcctt tttcttgcca cttttggcca gatttttcag cttttcgatt cctttccggc
    75061 gtaatcttac tttcaattgg tccaaaaact cacccttcct tctctcataa aatggtatag
    75121 gaaaaaaaga aaaatagcaa tcgaactttc aaataaatgc ataaatagaa taaaaagaag
    75181 acctagaaat gattggcctt ccttttttaa tgaatttcaa ctgttcagcg cgtgtttttg
    75241 tctgataaaa agtgcataca gaaaatatgg cgcaccaaaa agggcaatca aaattcctgt
    75301 aggtacttcc ccacttggta gaatcactct agcaaccata tctgcactaa gcagaaaaat
    75361 ggaaccgaac aaaccgctca gaacaaaggt ccagttattt tctttacgta cgactagctt
    75421 cgcagcatga ggagctagta gtcctacaaa agataaacta cctgtcaccg ctacactacc
    75481 gcttgccaaa gcaacagcta acaacaaaag gattttttga cttttagcca cgttaaggcc
    75541 taatccttgc gcgcgttctt gaccaaatga tagcaataca atcctctgtg cataaagtcc
    75601 tcctagcgga attaagataa ccgcccatgg aagcaatgcc caaacataag gccagctcgc
    75661 accccagatt gatcccgcta accatgcatt cacaaatcca taattttcct tagataattg
    75721 gatcgtgcat agcaatgtta atgcagaaag gccggcatta acggcaatac cggacaataa
    75781 gattcgattc atacccaaaa agccatttcg atcataggcc gcaagataga cacagcaagc
    75841 agctaaaaca ccacctagaa aggctacaac tagcaataga aacggcagcg aatagtgcga
    75901 aaagaaacct aaataaagca tcactaaaaa tcctgctccg gcattaatgc ccaatatact
    75961 agaatctgcc aattcatttc ttgttactcc ttgcaagata taaccagaaa cgccaagaca
    76021 agctcctgct aataaactga tcagtaatcg aggaactcga aattctctaa caatcagcgc
    76081 caaagaagat gattctttcc ctatgaacgt ggcaaaaagc tgatccaggt aaataaaagg
    76141 cgtaccaatc attaaactac ctatagacag gatgacaagt acaaaaagca agaaatagaa
    76201 cgtccaatat atttttttca ccgttgttcc ctccttactt gtatcagtaa aaacggaacg
    76261 ccaatcaatg caatgactaa tccaaaaggt gtttcaaaag ggggattgat catcctcgct
    76321 gtaaaatccg ccaacataac caatccaccg ccaactaaaa aagagacagg tagaacataa
    76381 cgatagtccg ttccaaccat tccgcgaaca actggcggaa ccaacagccc aataaatgaa
    76441 acaggtccga ctaacgaaac ggcaatggct gctaacactg caactagaca gaaaacaatc
    76501 aaccgcgttc gtttaggatg acttccaaga gctaatgcat gtgtttcact caaatgaatc
    76561 aaagatattg cttttcctaa tgaaaaagaa accaaaacag caagtatata taaaggaaac
    76621 aatactgcta attgtttcca tgtgatattg gcagctccgc ctacaaacca aaaagcaaga
    76681 tcctgctgca gattaaagaa ttgggtaagt gcttggctga tagcagtaaa aaaagaacta
    76741 atagctacgc cagctaaaat cagctgtaaa ggattcatgc ccattttcga aaactgcaaa
    76801 aaaccaaata cgagcaaagt tacagcaaaa gaacctgcca atgacattag aaacacagaa
    76861 aaatcagaag ctttaggcca aaaagcaaaa ctaagggcca ttgccaacga agcacccgcg
    76921 ttgattccta gtaagcctga atctgctaaa ggatttcttg tcactccttg catcaatgca
    76981 ccgctcaatg caaaacaact ccctacaaga aatgctcccg ccacacgagg caaacgaata
    77041 tttcgtatga tttggtgact ttgttttgat ctatcaaaat caaacaacgc ctgccaaaaa
    77101 tctgcttttt ccacagtcgc tgcgccattt gaaagagcaa caaaaaaaat aatcaaaaga
    77161 acaaaacaga ccaaaagcag gagtctattt ttcttcatag tcctcataat tgatcctcct
    77221 gctcttttgg caacaaatca taggttagaa aaactggttt atttgttctt ggaagcgtga
    77281 caatcgttgc atcaatctca aatatttttt tcatacactc ttttgtgatc acttcttcag
    77341 gtgtaccttc tgtgatcaac ttaccgtttt tcattgctaa taaataatct gaaaaacgtg
    77401 aagcatgatt aatgtcatga atcgacatca agatcgttgt ttgtttcatt tgattgattt
    77461 cagtcaataa ctgtaggatc tctaattggt gtgccggatc taaaaatgtt gtcggttcat
    77521 ccaagatcaa gatatctgtt tcctgagcaa gcgccatagc gatccagaca cgctgggttt
    77581 gtccaccaga cagcgttgcg atatcccgca tacgaaatcc agctagacct gtagcatcca
    77641 acgcccactc aattgcttca tgatcttgcg tgtccatgcc actgaacagt ttttgataag
    77701 ggtatcgtcc gtaagatact acttcttcta ctgtcatacc tgtagggtgt tcactagttt
    77761 gtgctaataa tgcaattttt tttgcaagtt cttttgaact gtatgtagag atatcagagt
    77821 tattgacaat gatctttcct gatttcacag gtagtatacg ggtaaaactt ttcaataaag
    77881 tagattttcc acttccattt ggcccaatca aactagtgat tttcccttca ggaatcgtaa
    77941 cagacatgtt ttcaatgatt tttttgtttt cgtagccggc tgcaatgtca gctgctctta
    78001 atctcgtcat ttcacccatc ctttcgctga aaatcattct caattatgac gctgtttcaa
    78061 catttcgtca agcgctactt tcatatctat agccaaatga agcggttgac agcgttcgaa
    78121 atatcaggaa taataaccaa tgagaaagat tctcaattat gttaggagga aagaaatgaa
    78181 attaaaaaca aaagtattca cattatctag catattgctg ctggctggag tactgagcgc
    78241 ttgctccccc tcttcagata aagaaacagc tacaactgcc aattcgacga cttcatccaa
    78301 tagtactcat acagtcacag atacacttgg acataaagta gagattccaa acgcgcccaa
    78361 acggatcatc ggcagttact tagaagacta tctgattgca ctaggggaaa aacctatcgc
    78421 ccagtggact gttggcagtg gttccattca agattatctg caagatgatt taaaggatgt
    78481 cccaacgatt tcttatgacc ttccttatga gaaagtgtta agctttgagc ctgatttgct
    78541 gttgatttct tcttccgcta ctgtcgaagg cgggaaatac gaacaatata gcaaaatcgc
    78601 gccaacttat gttgtaaaaa acggaacaga tgtcacatgg gaagaacaac taaaagatgt
    78661 cggcaaagct ttggcaaaag aaaaagaggc agaaaaaatc atttccgatt atcaaaaatc
    78721 catcaagaaa actcgagaag aactagcaga taaaatcgag aacaagacag ctgcagtgtt
    78781 gtgggtgacc aacaactcag cctttatggt cagcaaaaat cggtcaagtg gaagaatcgt
    78841 atacgacgat ctggaatttg gtgttcctga tctagtggaa gaggtctcca aaaaagccac
    78901 ttctgactgg tctgctgttt cctctgaaaa gctagctgaa ttagatgctg actacatcat
    78961 ccttgtgaac agtgacaaag atgctgccat gttcaatgaa ccaagctgga agaatctaaa
    79021 agcagtgaca gacaatcatg tactgcaatt cggacctgaa tctagttggc tatataacgg
    79081 acctattgcc agtcaggaaa tggtcaatga tatcaaagaa aaactaaatt gattttaact
    79141 aaaaaaagca tcttcacgat tagtcgtgag atgctttttt tcttattaac aaactagacg
    79201 catagatttt attcttcttt tgaagcaacc ctatttttta ctctttccca tttttgaaaa
    79261 cgatgggcat atagattttc ttgatccgtt atcccctcat actcctctat aagggcgaag
    79321 tctgaaaaat caatattccc aatatacgta tcgccttcaa acgtgctatc gatcaatgtt
    79381 cgccagatcc gaccagtgta aggaagaagt tcttgaaaaa tccgactacc acccgaaaca
    79441 tagatatctt ctgagatcgt ttgactatac gacaaaagtt catcgatcga atgcattact
    79501 tccgcatttt ctttcgtgcg aaaatttggt tgcgttgtca acacgatgat atggcgataa
    79561 ggaagtgaaa gatcccccat tccttcatag gtctttcttc ccatcactaa aattcgatcc
    79621 attgtatgtt cgcgaaagaa acgcatatca tttggcaatc gccatggtaa caaaccattt
    79681 ttgccgatca gaccattttt atcttgtgcc cacatggaaa taaacatatt atccgctcct
    79741 tatgatttta gatgttttga gataaaaaaa gtctcaattc tttgaccagc aaggaattga
    79801 gaactttaaa aacattattt tgtttcacgg tgcaaagtaa ctttgcgttc gcgtgggcaa
    79861 tatttttgtt tttccaaacg atcaggattg ttacgtttgt ttttgcttgt tagatagtta
    79921 cgttctttac aagaagtaca ctctaaagtg atgtttacgc gcatgttttt ccctcccatt
    79981 tttacaagaa tcctctaaga tgactctcag acttgattat cttaccatga ttttttctag
    80041 attgctagtg tttttcacga ttttgttaag taaaactact tgacgataaa tacaacggtt
    80101 ttacttctca aacaaaatct ttttcttttt gtataaatac ccttacactt gtttatatag
    80161 attatgtttt ttaataaagg gggatactaa tatgccatgg aacatgaagg actttcctgc
    80221 atcaatgaaa aacttagata aattgacaag aaaaaaagca atcgacatcg caaatgcatt
    80281 attagatgaa gggtaccctg ataaccgtgc gatcccaatt gccattgatc aagcaaaaaa
    80341 atggtatgaa gatgctagtg aatcagaaaa acgaactttc gaaaaagaaa aaaatccaag
    80401 caagacggat gaacacgata cgaatcctcg tgctggaaaa ctgcttgatt cagatgtgat
    80461 cgtagaatat gaggaagaac aatggatcgt gaaatctaaa ggggcaaaaa aggcaagcaa
    80521 ccattttgat acaaaaaaag aagcaatcga aaaaggaaag caagtagcac aaaacaaaga
    80581 atcaactttg ttcatttata aaaaagatgg gacaaaagaa aaagaaatcg attattgaac
    80641 atatgtaaag gggttgtgac gaaaatcgtg tcacaacccc cttatgttcg aaacaatggt
    80701 gttcctctgc caactccgaa ccataagtca taatatctat aaaacgggaa gagctgtttt
    80761 ttgaaattgt ttcttatcac tcagtgttaa acggtaggag aaaacctctt ttttattgat
    80821 atttgatttt catcaaacag tcacatagtc gattgcttct gcttgcggaa tcgtttcatc
    80881 tacaaagtag actttatacc atgtcaagaa actgaagatt gtccagattt ttctttgctc
    80941 ttctttacgt ccttcgtgat ggtcatctag caacttcatg attttttctt gatcaaaaaa
    81001 ttcttttgca aaatctgctt cgaataatgc tttgacttgt tcgtagccgt gttcttcttt
    81061 caaccaagct ttgattggca cagggaatcc taatttcaca cgattcgacc attcttccgg
    81121 aagatgtttg ttcgcagctt gtctgaaaat atcttttgtg ttgttttgat tcaataaaaa
    81181 cttacttggt atttgttgag ccaatttcat gacttcgata tccaataaag gtactcggac
    81241 ttccaaagaa cttgccatag aaagtttatc tgcctttaac aagatatctt ttggcatcca
    81301 ttgatgaaga tcgacatatt gcatcttatt gacttcatct ttgattcctt ctgttttctc
    81361 gtaatgaaca gtcatgattt catccacaga tggtgctgtt tgatattttg gtgttaacac
    81421 ttctgatgct tctgattctt cgaacactcg agcatgaccg ataaaatatt cttttgcagg
    81481 agcaagtgat tcatataaat ggatccgtcc atggaagttt ttcatttttc cgattttacg
    81541 tcctaaatta tagcgtgttc cttttggtaa ttttttcaat ccttgagcaa tcacacggat
    81601 gaattttgaa ttcgtatgga atccatacgt ttggtatccc gcaaacagtt catccgcccc
    81661 ttctccagat tggactaccc gcacactttg tgaagcaaga tttgctaaaa agtacaaagg
    81721 tacgcaagaa ggatttgaat caggttcatc taagtgatac tggatcaaag ggaatgcttt
    81781 gaacgacatg tctccgtcga tcactgctgc tgtattatct aagtccaata gttcggttag
    81841 ttttctggct tcaatcgctt cattataggt tttatcatca aacccgattg agaaagaatg
    81901 atctggtttc aatacagagg ttacataact ggaatcaacc ccgctggata agaaagaacc
    81961 aacttctaca tcactgaccg tatgtgcttc aatagaagct tgaacggttt cgtcgatttt
    82021 ttcgatccat tcttgtcggg aatgcgtttc ttttgtttca aaatctgcat cccaatattg
    82081 ctgaatgtct atttttccat ctttatacgt ataataatgg ccttcaggca aacgatagac
    82141 atctttgaag aatgtttcgc cgtttaatgg agaatattgg aaagtcatat aaggtttcaa
    82201 cgcttctttg ttcaattctt tgttaaaatc aggatgaggc agaaaacttt tgatttctga
    82261 accaaacata aatgttccgt tcatttctgc ataataatat ggtttgatcc caaagtggtc
    82321 tcttgcaccg aataactcat tcttttcatt atcccaaata gcaaaagcaa acattccgcg
    82381 gatcttttgc aataattctg ttccccattc ttcgtaacca tgcaaaagga cttctgtatc
    82441 tgcatgtgtt tgaaaaacgt ggccagctgc aatcaattct tctctcaaag gctggtaatt
    82501 atagatttcg ccattaaaaa taatgatctt tgtccgatct tcattgtaaa taggctgagt
    82561 accgccctct aagtcaataa tacttaatct tctgaatcct aaagcaacat gtttatcgat
    82621 atattctcct gaactattag gtccacggtg aacgattcga tccatcattg tattaataat
    82681 tgtttttttg ttgtccttgt cgttaacaaa acctacaata ccgcacattt aactttcaca
    82741 tcctaatata aaattactgt ttatttcatt catatgcatc ttatcataga ataaatagcg
    82801 gacgaatatt ttctctctta caatttcgtc acgtgaaaac aaaagatgaa agtttctgtt
    82861 tttacagaaa ataaagtccc aatatctaat ttaccatgtt ttcttttttg aggctacctt
    82921 attctcttcg tgttaaaaaa atattagtgg atgctatttc caaaaaaacc agcaaaaaaa
    82981 gacacgacct aaagatcgtg t
//
LOCUS       DANKMN010000004        62054 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000004 DANKMN010000000
VERSION     DANKMN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            184..702
                     /locus_tag="PW224_000256"
     CDS             184..702
                     /locus_tag="PW224_000256"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2906975.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            830..1513
                     /locus_tag="PW224_000257"
     CDS             830..1513
                     /locus_tag="PW224_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL2906976.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1822..1895
                     /locus_tag="PW224_000258"
     tRNA            1822..1895
                     /locus_tag="PW224_000258"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1856..1858,aa:Arg,seq:tct)
     gene            2054..2413
                     /locus_tag="PW224_000259"
     CDS             2054..2413
                     /locus_tag="PW224_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL2906977.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2536..4167
                     /locus_tag="PW224_000260"
     CDS             2536..4167
                     /locus_tag="PW224_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL2906978.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4229..4870)
                     /locus_tag="PW224_000261"
     CDS             complement(4229..4870)
                     /locus_tag="PW224_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2906979.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5056..5132
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5152..5460
                     /gene="rplU"
                     /locus_tag="PW224_000262"
     CDS             5152..5460
                     /gene="rplU"
                     /locus_tag="PW224_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL2906980.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5487..5831
                     /locus_tag="PW224_000263"
     CDS             5487..5831
                     /locus_tag="PW224_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL2906981.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5844..6137
                     /gene="rpmA"
                     /locus_tag="PW224_000264"
     CDS             5844..6137
                     /gene="rpmA"
                     /locus_tag="PW224_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL2906982.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6571..9378
                     /locus_tag="PW224_000265"
     CDS             6571..9378
                     /locus_tag="PW224_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2906983.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9569..10060
                     /locus_tag="PW224_000266"
     CDS             9569..10060
                     /locus_tag="PW224_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2906984.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10163..11152
                     /gene="manX"
                     /locus_tag="PW224_000267"
     CDS             10163..11152
                     /gene="manX"
                     /locus_tag="PW224_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL2906985.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11185..11988
                     /locus_tag="PW224_000268"
     CDS             11185..11988
                     /locus_tag="PW224_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL2906986.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            12009..12923
                     /locus_tag="PW224_000269"
     CDS             12009..12923
                     /locus_tag="PW224_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL2906987.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            13006..13380
                     /locus_tag="PW224_000270"
     CDS             13006..13380
                     /locus_tag="PW224_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL2906988.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13519..13764
                     /locus_tag="PW224_000271"
     CDS             13519..13764
                     /locus_tag="PW224_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2906989.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13936..15000
                     /locus_tag="PW224_000272"
     CDS             13936..15000
                     /locus_tag="PW224_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2906990.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15246..15671
                     /locus_tag="PW224_000273"
     CDS             15246..15671
                     /locus_tag="PW224_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2906991.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15664..16113
                     /gene="nusB"
                     /locus_tag="PW224_000274"
     CDS             15664..16113
                     /gene="nusB"
                     /locus_tag="PW224_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL2906992.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16210..17064
                     /locus_tag="PW224_000275"
     CDS             16210..17064
                     /locus_tag="PW224_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL2906993.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17065..18402
                     /locus_tag="PW224_000276"
     CDS             17065..18402
                     /locus_tag="PW224_000276"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL2906994.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18407..18631
                     /locus_tag="PW224_000277"
     CDS             18407..18631
                     /locus_tag="PW224_000277"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL2906995.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18633..19523
                     /locus_tag="PW224_000278"
     CDS             18633..19523
                     /locus_tag="PW224_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2906996.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19532..20350
                     /locus_tag="PW224_000279"
     CDS             19532..20350
                     /locus_tag="PW224_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL2906997.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20457..20906
                     /locus_tag="PW224_000280"
     CDS             20457..20906
                     /locus_tag="PW224_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2906998.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20924..22606
                     /gene="recN"
                     /locus_tag="PW224_000281"
     CDS             20924..22606
                     /gene="recN"
                     /locus_tag="PW224_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL2906999.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22661..22777)
                     /locus_tag="PW224_000282"
     CDS             complement(22661..22777)
                     /locus_tag="PW224_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL2907000.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22850..23806)
                     /locus_tag="PW224_000283"
     CDS             complement(22850..23806)
                     /locus_tag="PW224_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2907001.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23977..24345
                     /locus_tag="PW224_000284"
     CDS             23977..24345
                     /locus_tag="PW224_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL2907002.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24515..24946
                     /gene="mraZ"
                     /locus_tag="PW224_000285"
     CDS             24515..24946
                     /gene="mraZ"
                     /locus_tag="PW224_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL2907003.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24967..25926
                     /gene="rsmH"
                     /locus_tag="PW224_000286"
     CDS             24967..25926
                     /gene="rsmH"
                     /locus_tag="PW224_000286"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL2907004.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25931..26332
                     /gene="ftsL"
                     /locus_tag="PW224_000287"
     CDS             25931..26332
                     /gene="ftsL"
                     /locus_tag="PW224_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2907005.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26333..28525
                     /locus_tag="PW224_000288"
     CDS             26333..28525
                     /locus_tag="PW224_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2907006.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28551..29513
                     /locus_tag="PW224_000289"
     CDS             28551..29513
                     /locus_tag="PW224_000289"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL2907007.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29527..30888
                     /locus_tag="PW224_000290"
     CDS             29527..30888
                     /locus_tag="PW224_000290"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL2907008.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30914..32002
                     /gene="murG"
                     /locus_tag="PW224_000291"
     CDS             30914..32002
                     /gene="murG"
                     /locus_tag="PW224_000291"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL2907009.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            32017..33237
                     /locus_tag="PW224_000292"
     CDS             32017..33237
                     /locus_tag="PW224_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL2907010.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33393..34718
                     /gene="ftsA"
                     /locus_tag="PW224_000293"
     CDS             33393..34718
                     /gene="ftsA"
                     /locus_tag="PW224_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL2907011.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34741..35982
                     /gene="ftsZ"
                     /locus_tag="PW224_000294"
     CDS             34741..35982
                     /gene="ftsZ"
                     /locus_tag="PW224_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL2907012.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35999..36676
                     /locus_tag="PW224_000295"
     CDS             35999..36676
                     /locus_tag="PW224_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL2907013.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36690..37289
                     /locus_tag="PW224_000296"
     CDS             36690..37289
                     /locus_tag="PW224_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL2907014.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37304..37591
                     /locus_tag="PW224_000297"
     CDS             37304..37591
                     /locus_tag="PW224_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL2907015.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37640..38422
                     /locus_tag="PW224_000298"
     CDS             37640..38422
                     /locus_tag="PW224_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL2907016.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38443..39150
                     /locus_tag="PW224_000299"
     CDS             38443..39150
                     /locus_tag="PW224_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL2907017.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39412..42210
                     /gene="ileS"
                     /locus_tag="PW224_000300"
     CDS             39412..42210
                     /gene="ileS"
                     /locus_tag="PW224_000300"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL2907018.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42259..43785)
                     /locus_tag="PW224_000301"
     CDS             complement(42259..43785)
                     /locus_tag="PW224_000301"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL2907019.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43800..44447)
                     /locus_tag="PW224_000302"
     CDS             complement(43800..44447)
                     /locus_tag="PW224_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL2907020.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44631..44960)
                     /locus_tag="PW224_000303"
     CDS             complement(44631..44960)
                     /locus_tag="PW224_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL2907021.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45137..45865
                     /gene="sfsA"
                     /locus_tag="PW224_000304"
     CDS             45137..45865
                     /gene="sfsA"
                     /locus_tag="PW224_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL2907022.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45881..46894
                     /locus_tag="PW224_000305"
     CDS             45881..46894
                     /locus_tag="PW224_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2907023.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46894..48171
                     /locus_tag="PW224_000306"
     CDS             46894..48171
                     /locus_tag="PW224_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2907024.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48234..50936
                     /locus_tag="PW224_000307"
     CDS             48234..50936
                     /locus_tag="PW224_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2907025.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51088..51405
                     /locus_tag="PW224_000308"
     CDS             51088..51405
                     /locus_tag="PW224_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2907026.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51435..51755
                     /locus_tag="PW224_000309"
     CDS             51435..51755
                     /locus_tag="PW224_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2907027.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51863..53323
                     /locus_tag="PW224_000310"
     CDS             51863..53323
                     /locus_tag="PW224_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2907028.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53391..53612
                     /locus_tag="PW224_000311"
     CDS             53391..53612
                     /locus_tag="PW224_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907029.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53643..53825
                     /locus_tag="PW224_000312"
     CDS             53643..53825
                     /locus_tag="PW224_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL2907030.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53825..54238
                     /locus_tag="PW224_000313"
     CDS             53825..54238
                     /locus_tag="PW224_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL2907031.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54361..55542
                     /locus_tag="PW224_000314"
     CDS             54361..55542
                     /locus_tag="PW224_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2907032.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55613..55777)
                     /locus_tag="PW224_000315"
     CDS             complement(55613..55777)
                     /locus_tag="PW224_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907033.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55931..56004
                     /locus_tag="PW224_000316"
     tRNA            55931..56004
                     /locus_tag="PW224_000316"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55965..55967,aa:Arg,seq:cct)
     gene            complement(56073..57212)
                     /locus_tag="PW224_000317"
     CDS             complement(56073..57212)
                     /locus_tag="PW224_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2907034.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57511..58146)
                     /locus_tag="PW224_000318"
     CDS             complement(57511..58146)
                     /locus_tag="PW224_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907035.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58259..58894)
                     /locus_tag="PW224_000319"
     CDS             complement(58259..58894)
                     /locus_tag="PW224_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDL2907036.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58928..59389)
                     /locus_tag="PW224_000320"
     CDS             complement(58928..59389)
                     /locus_tag="PW224_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDL2907037.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59519..59950)
                     /locus_tag="PW224_000321"
     CDS             complement(59519..59950)
                     /locus_tag="PW224_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2907038.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59968..60288)
                     /locus_tag="PW224_000322"
     CDS             complement(59968..60288)
                     /locus_tag="PW224_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2907039.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60587..61363
                     /locus_tag="PW224_000323"
     CDS             60587..61363
                     /locus_tag="PW224_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL2907040.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61378..61581
                     /locus_tag="PW224_000324"
     CDS             61378..61581
                     /locus_tag="PW224_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2907041.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61597..61935
                     /locus_tag="PW224_000325"
     CDS             61597..61935
                     /locus_tag="PW224_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDL2907042.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT        20465 a        10429 c        13027 g        18133 t
ORIGIN      
        1 tgttagggta aacgtttttt aaactaacgt caattattta taataaaaaa tgagataaag
       61 atgaggaaat agaattagaa acatataatt attaaaggga aacattttta cagctataat
      121 ttgaaaaaat ttatcgttat tatatgatta agaaggattg aataaagaaa gaagttgaaa
      181 acgatacctg attacaaaaa aataactttt ataagttcta taacgttgat tatattttta
      241 attcttattc ttctcgtcgt cttggttccc cgaatttttg gtttgactcc ttatattatt
      301 gaggacaaag aaatgagtcc atattatgca caaggaaatc tgatatatgt acgaccgaaa
      361 aatacaaatg agattttagt cggagatatt attacatatt atgaaaattc aggaaaaaaa
      421 atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg aagattttta tacaaaagct
      481 gatacaaaga cttatattga gcctggagta gtaaaaaaga gaaatattat cggtagtccc
      541 atctttcaga ttccatatat tggactagtc ctttcaaaaa cggcatttgc atggattcag
      601 gggttattct ttacgattgc tttttgccta acaggaatca cagcatggaa tacctttgta
      661 gagttgaaaa agaagcggag acgaacacag aaatttttgt aagaatgtta aaaacataaa
      721 tgtattccag agatccaacg actcttctga acatgaaaca cgagaaaaca ctcatgcagc
      781 ttgtagattt agtcagttgt tcgtataatg tgtgaagagg aggagtagta tgcgcaatga
      841 aatgatgcat cgtcctgtag tcaaagaaga actattggat tttatgcgta cccagcaaaa
      901 aaaattaccg ggtgagctag gcaaactgga agaagaagct catgtagcag aagtaccagt
      961 tattccccat gaaacagttg tatttttgaa atttttactt ggacagcttc agcctgaacg
     1021 aatcttggag atcggagcag ctatcggttt ttcatcaagt gtcatggcca cgacaatcgg
     1081 agaaaacgct catgttacga caattgatcg tttcgatgtc atgatcgaga aagctaaaaa
     1141 aaactatgaa cgtctaggac tgacagataa agtgactttg cttgaaggtc aagcagcaga
     1201 tatcttgccg gagttgtctg gtccgtatga ctttattttt atggatagtg caaaatcaaa
     1261 atatattgaa tttttacctg aatgcttacg tttgttaaga aaaggtggcg ttttgatggt
     1321 agatgacatc ttccaaggag gaacgatctt gctcccagat gaagagattc cccgtggcaa
     1381 acgagctatc catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc atcccgattt
     1441 gacatcgaca atcttgccat taggtgatgg tgtgatctta atgacaaagg aatcagaaac
     1501 aatcgagttg tgatacatga ttatggcagc ctgtagataa tcgattgaat cgagggctca
     1561 attcgaactt gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata caggaaatct
     1621 aaataaacag gagtagcatg aagtcaatgt agctcctgtt ttcaattgat aaccttagaa
     1681 cagctgaagg agaatgtctc gttttaaaag attgatttag tagttgggcc aaaataattc
     1741 attatttgct aattaaataa ctatttcgta ttgacagaag caatttaatt atctataatt
     1801 attgatgttc cgctgattct tgtcatagta gctcagctgg atagagcatc cgccttctaa
     1861 gcggacggtc gggggttcga atccctcctg tgacgctaaa aaaccgttag aaactgaggt
     1921 ttctaacggt ttttctttgt tttttgtgcc aatattgttt gttttcaaag gcaaataaaa
     1981 taaaaaaaca tccttatcga gtacaattat aaggagcaaa aaaataaaac tcatcagaaa
     2041 agaggaagaa gatatgacga tactatctag aattttagtg actctagttg caattgaatt
     2101 tttttatatc atgtatatcg aaacggtgag aactgattct gacacgacaa gtagggtttt
     2161 taaaatgagt aaagaagagt taagcagaaa atctgttcaa acattgttta agaaccaagg
     2221 agtgtacaat ggactattag gagtgggact actttatgga gcttatcttt cttccgcatc
     2281 taaagaaatc acaagtatgc tattaatatc tatttttttt gttgcattat atggcagttt
     2341 gactagcgat cgttggattt tactcaaaca aggtgggcta gctctgttgg cattgctttc
     2401 tattttctta taaatataga aaaaataaga atttatctaa caggaaactt tgtgaaaaat
     2461 attactttat ctcatgaaat atgatattct aatgaatggt attttagaaa atttcatgaa
     2521 aaaggagtta gataggtgtt caaaaattat gtttcctcac taaaaaaaga attttcaggt
     2581 tatgatcttt caagatttca gaaagatctt ttggctggga tcacagtagc tgctgtagca
     2641 ttgcctttag ccttggcttt tggggtatca agcggagctg atgcggccgc aggtttgatt
     2701 acggctatca ttgccggtat cgttattggc ggtttatcag gcggttttta tcaaatatca
     2761 ggtccgacag gtgcgatggc agctatcttg atgtctattg cggcaacaaa gggaatgcaa
     2821 ggtgtattgc ttgctacttt tttagcgggt ctatttttac ttgcagcagg acttttccga
     2881 ttaggaacac tgacttcttt tatcccagca cccgtaatta cggggtttac ttcaggtatt
     2941 gctatcatta ttgcattagg tcagatcgat aatttatttg gtgttcactc ggaaggtaca
     3001 aacgttattg aaaaactaag tagttatcaa acgataggat ttccgataaa cacagcctct
     3061 ttgatcatgg gagggctagt cattttaggg atgtttatct atccaaaaaa atgggcgaaa
     3121 cgaatgccta gttcgctgct ggctatcatt ttggcaacag cagttatgct attgttccat
     3181 ttaccagtag caactgtggg taagattcca caaacattga tcagtagtaa tcgtttgaat
     3241 atgggagatt ttagcttttc tgccttgcaa caagtggcag ttccagctat tagcattgct
     3301 ttattaggaa tgattgaaag cttgctatgt ggtgcttcgg ctggccgaat ggcaaataag
     3361 cccttagata gcaatcaaga attaatggcg caaggaattg gaaatctgtt gcttcctttc
     3421 tttggcggga ttcctgctac tgcggccatc gcccgtacaa gcgtggccat caaatctggt
     3481 gcacaaacta gattagctgg tatgatacat gcagttgtct tactgctctc catgcttgtt
     3541 tttgcgccag tgatgtcaca gattccaatg ccagcacttg caggtgtttt gattgtgacg
     3601 gcttggcgta tgaatgaatg ggaaacgatc aaggagctat ttactaataa atattggtca
     3661 gcagttctat tgtttatcct tacgatgatt tgtacagtta tctttgattt aagtattgct
     3721 atcgtgatcg gggtgatcgg cggctgtgta ttttttgttg tcaaaagcgc tgctatcacg
     3781 atttcattag aaacgatcga ttggcaaaga atggggctgc cagaaacgga aaagctgaat
     3841 aactggacag tagtgtatat tagtggtcca ctgtttttta tgtcttcaga aaaactcaaa
     3901 aataaattgg aagaactaaa tcatcacgat ggtgtcattt tctccatgcg aggcgtacca
     3961 agtattgatt tgacggcacg tgcagttttt gatgaatttc aagaaaaagc agagcaaaga
     4021 ggacaaacga ttatctatac ttccttgcag ccagaagtag aaaaacaact ttctcacttg
     4081 tgggaacata cattcacgca tcaacatcaa acagttgctc atgcgttgac agctttacat
     4141 gcgaaatata ctgcaattag ccattaaaac aatgaaaaag aacagacaag actgtaaact
     4201 gaagatttta tggtcttgtc tgttcttttt aatattgctg taaaagtgaa gctagtactt
     4261 gccagtcttg atggataaca ggtacaaggg agcgattgta gaaaatagct gctggatgaa
     4321 aaagtggaag aattcgatag tttttttctg accattcgta ggcatccttt tgttctgtta
     4381 attgtaagac aggccctgta tagagttgac catgattttt ggtaaccgta tattctttgc
     4441 caagtaaacg ttgaagacca atgtttccaa gtgtcgcaat taattttggc tggaccttca
     4501 tcagttcgta atccaatatt ggtgcatgcg caaatacttc ggaacgagta ggtgtccgat
     4561 ttggataaac agtttctgac tgttgtgtcc gtttattgat ccgatgagtc acgcgatacg
     4621 ggcggctcct tacagcactg gtgatataca cctcttctcg tgttaagcca gttgaagaaa
     4681 gtgcctgcat caattctttt ccagcttgac cgctaaaagg aacatgcgta tcaatctctg
     4741 ttttccctgg ggcttctccc acgagcatca gtttaggatg aagaggtcct tgcccagcga
     4801 taaatccttc tacaggacgg ccgttcattc ttaaagaagc ttcttgtatc agttcttttg
     4861 ggtattccat ttatgctcct cctttttctt ttattataag aataaaagaa acacaatgcg
     4921 actattttat ttggtagaac tgtgaggata gaaaagcaga agtattgctt gaatttttgt
     4981 tttacttttt gattttaaat ttagttgact aacggttgct gttctggtaa gctagtactg
     5041 ttgtaattgt ggactccaca gctacaaccg ctcagaacaa gtaataagca cagcatatgc
     5101 tgtcacttcg gatggcgagt ctaagtatat cagaaggagg tgctttatag catgtacgca
     5161 attattaaaa ctggtggtaa acaagtaaaa gttgaagtgg gacaagcaat ctatgttgaa
     5221 aaacttgacg ttgaagcagg agaaaaagtt gtttttgacg aagttatctt agtaggcgga
     5281 gaatctacaa aagtaggcgc tccaactgtt gcaggtgcaa ctgttgaagg tactgttgaa
     5341 aaacacggca aacaaaagaa agtcgttact ttcaaataca aacctaaaaa acattcacac
     5401 cgtaaacaag gtcatcgtca accatataca aaagtggtta tcgacgcgat caacgcttaa
     5461 tagcgtttga gaaagaaggc ctatatatga tcaaaggaac atttaagcga aatgacgcag
     5521 gtcagatcgt ttcattcact ttgaccgggc atgctgatgc tggtccttat ggaagtgata
     5581 tcgtttgtgc gggtgtttct gcgttagcga tcagcactgt caacgggatt gcctcattag
     5641 ccggttttga gccgattgtt gaaatgaatg aagaagaagg cggttatctt tataccgaag
     5701 tcacttcagg aatgacacaa gaacaaaaca atattgctga gattctatta gaaaatcttt
     5761 tattaggatt acaatcgata gaagctgaaa actcagaata tatccaaatt aaaacaatta
     5821 atgataaata ggaggtgcag accatgttgt tatctatgaa tctacaatta tttgcccaca
     5881 aaaaaggtgg cggttctaca tctaacggcc gtgactcaga atcaaaacgt ctaggtgcta
     5941 aacgtgctga tggacaaaca gttacaggtg gatcaattct ttaccgtcaa cgcggtacaa
     6001 aaatctatcc aggtgtaaac gtgggtatcg gtggagacga cactttattt gcaaaagttg
     6061 atggcgtagt acgtttcgaa cgtaaaggcc gtaacaaaaa acaagtgtct gtttatccag
     6121 tagctcaaga agcttaattg aatcttatga ttaaagaggt tgtgacaaga cttttgtcac
     6181 agcctttttt gtttataaaa ataaaagttg tagtggaggc tacgaagttt actttgtctt
     6241 tttacactta atattttttt gccaatttgg aatcaggatg ttttgataac tatcgttttc
     6301 aattgatgga agctatattg tttgctatca gtgaaataat tatatggtaa acagtgttct
     6361 ttttcttgtt tccattataa aaatcgtatc gggatttttc gttcttttaa attgaacatt
     6421 tctatctatc cgcgtgttat caacaaaaca aaatcaggta aatagttgac gaaacagaaa
     6481 taaatacata gtattatgtt ttgaagccta agtacaatag tctatagaaa aggagcgata
     6541 ccattggcaa aacgcattga ccgaatttat atgtatgtca aagaaaaaac cgctcattta
     6601 acaaaggcag aatatgatca aggagtaaca acacaggaaa ttgctgaagt gttagggatt
     6661 cagcgaacaa atagcagtaa agatctgaat cagctagtgc gcgaaggaaa actgataaag
     6721 acagacggac gaccagtacg ttacatatat caaacactcg ttacgcacgt aaagccagtt
     6781 acaaaacatg tagtcagtta caaagaaacg ccggtctctg aagaaaaaaa tacactccaa
     6841 atagatacta aggatatctt tgcaaaaatc attggagcta acggcagcat gaaaaattca
     6901 gtggaacagg caaaagctgc gattttgtat ccgccaagag gattaaattg tttgattaca
     6961 ggacctaccg gatcaggaaa gacttatttt gcacacgcga tgtatcattt tgcaaaagcg
     7021 catcatgtaa tagatgagga aacggaactg attgttttca attgtgctga ttatgccaat
     7081 aaccctgagc ttttgatgag tcatttattt gggtacgcaa aaggtgcttt tacaggcgca
     7141 gatgaagaaa aaatggggat tatcgatcaa gcggatggcg gtatgctgtt tttggatgag
     7201 attcaccggt tgccgcctga aggtcaagaa atgatctttt actttatgga tcatggaact
     7261 tacagtcgtc ttggggaaac aacgaaatcc catgaagcaa atgtacggat cgtaggtgcg
     7321 accacagaag acccaggttc ttctctatta gaaacttttg ttcggcgtat cccaatcaat
     7381 atcaaacttc cggcttttga aaaacgtcct gcaaatgaga aagtcgattt agtaaaaatc
     7441 atgattgccc acgaagcaaa ccgcacgcag cgaaagattt ctttgacaga agatgtagca
     7501 aaagcattga tcggcagtgt gacctatgga aatataggac aattgaagtc aaatatccag
     7561 ttagtatgtg cacgaggatt tctaaatcat atgcaatcac ccgaaatctc gatcacaatc
     7621 gatgatttga cagaagggat tcgtagcgga ttgattcaat tagcaagcaa ccgcagtgca
     7681 atggcagagc tttctaaaat attggagcca aagataactg tagcaccaaa tgatacgatc
     7741 atgaaaatcc aatcggactc ctatgagtta ccttataatc tatatgatat catcggagat
     7801 aaagcagctt tactgaagtc ggatggtttg gatcaagaag caatcaatca tttcatttct
     7861 acagatatca acatccatct gaagtctttc tataaagacc atggtttttc ttttaatgca
     7921 gacagcaaat tagcagaatt tgtagatccg aaaatcatag aagtgaccaa tcagatttat
     7981 gtaatggtca aaagatcttt accatatgag tttcagcaaa actttattta tgctatgagc
     8041 ttgcatatca gttctttttt gaaaaaaatc aacttaggag aagaacggca tacgaatgat
     8101 aacatccgtg agatggcttt tgattttcca gatgaatatg ctgttgcgaa agaagtgcgt
     8161 agttatatcg aaacgtattt tcaagtggag attccagaaa gtgaagacta ttacttgacc
     8221 gttctactcg tctcgttacg ggcaaatcaa gcaagcggca gaatcggtgt agtcgttgca
     8281 gctcacggaa acagcacagc aagcagtatg gtccaagttg tccaacagtt gttagatgca
     8341 gagaacgtac gagcagtaga catgcctttg gatatggatc cgaaaacagc attggaccga
     8401 attgaacgca atgtacaaga agtagatgaa ggaagcgggg tcatactctt agtagatatg
     8461 gggtcccttg cgtcgtttaa ttcgcaaatt caaagagaca ctggaattcc agtacgtact
     8521 gttgatatgg taacaacttc tctagttttg gaaactgtac gaaaggtctc agtattaggg
     8581 acagatctag atatgttgca tgaatcattg aaaaattttc gaggttacgc agaaatatct
     8641 tctgtagaat ctgttcagga agcaaaacct attcataaga aaaaagctgt tttggctatc
     8701 tgcgcatctg gcgaaggtac agcacaaaga atcaaagaat tgatcgaacg agcggtaaac
     8761 aagcgtcaag aaacagaatt agaagttcta gctttgtcga ttgtggaagt gaaagaagaa
     8821 ctgccagtta tccaaaaaga atatcagatc atagctacta ctgggattac tgatttgaat
     8881 atccaagccc catttattcc tttagaacgt tttattgatc aaaatatcga agtcattttg
     8941 gatcagttgc tgatggaatc agaattggag gaaactgaat tcatctccct agatgaagag
     9001 tcagctaaaa atacgtgtgt tgagtttatc tctgataact tcatctttat taatggtagt
     9061 aagttaatcg atccaatgtg gcaattttct acacaaatct ctcagacgac gggaatcggc
     9121 gatgaagaat atggattcaa aatcaaccta gtgatgcaca cagcaggaat gatcgaacgt
     9181 attattcgga atgaaccatt gactgtggaa gaaaacgaat tgacaaatac aacgaatgat
     9241 cctctttata gccaattggc agcctcagtt gttctgttgg aagatcagat aaaagtaaaa
     9301 gtgccgatag aagaaatgta ttatctgcta cgtctagtcc ataatcagtt agataaaaaa
     9361 gaatacactg tgccatgaat acacaaaaac gatacacttt tatagtgtat cgtttttgtg
     9421 tattggttaa gaatccttta aattcaatta gtgtatcgta aaaaagttgg cacgcttttt
     9481 gcttatagta gtgtgtgcta ggaggtggtt gttcacacag aaatgatagg ggtttctgtg
     9541 aagtagtgag gaagaaggag ttttgcagat ggatattcga ttggcacgta tagatgatcg
     9601 tttaatccac gggcaagtcg caacagtttg gtcgaagcaa acaggtgttg aacgtatcat
     9661 tgtgatcagc gatgtagtcg ctcaagataa attgagaaag tttctactaa aagaagctgc
     9721 accgccgggg atcacagcaa atgtaataac cgtgcagaaa atgattcaaa tctatcatag
     9781 tggattatta tcacgagcaa aagtgatgct tttattcacc aatccccaag atgttgaagc
     9841 ggttgtccgc tgcggtgtac agttgaagtc gttgaatatc ggcggtatga gttttactga
     9901 aggcaaatca atgattacca attttgtttc agtaaatcaa caagatattg attcctttcg
     9961 ttacttgaac agtcaagaag tggagttaga gattcgaaaa gtacctgctg acaggaaaat
    10021 ccttttgatg gaactactag cgaaaatcaa aaaggtgtag caagaatggt tgttggaaaa
    10081 ggtcaaataa ttctgttgta atggttagca attattaata tttttgaatt tgttacaaca
    10141 aaagtaaata ggaggtataa atatggtagg gattatccta gcaagtcatg gacaatttgc
    10201 tgaaggaatc ttacaatccg gttcaatgat ctttggcgaa caagaaaacg tcaaagccgt
    10261 gattttgaaa ccaagtgaag gtccagatga cttgagagcc aaattggaag aagcggttgc
    10321 ttcttttgat aatcaagacg aagtgttatt cttagttgat ttatggggcg gaacaccgtt
    10381 caatcaatca aacacattat tcgaagagca caaagataaa tgggcaatcg tcagcggttt
    10441 gaatttacca atgttgattg aagcctacgc atcacgcttt tcaatggaat ctgctcatga
    10501 aattgcagca cacatcattg aaacagcaaa agacggtgta aaagtaaaac cggaagaatt
    10561 agaaccggca gaagcaccga aagcagcagt tgaagacgca caacctaaag gagcacttcc
    10621 agaaggaact gtggtcggag acggaaaaat caaatatgta ttagcacgtg ttgactcacg
    10681 tctgttacat ggacaagttg cgacagcttg gacaaaagca gtgcaaccaa accgtattat
    10741 cgttgtttca gatgcagtat caaaggatga tctgcgtaag agactaatcg aacaagctgc
    10801 tcctccaggt gtaaaagcaa atgttattcc tattagcaaa atgatcgaag tagcaaaaga
    10861 ccctcgtttt ggaaatacca aagcattgct tttatttgaa aatcctgaag acgtattgac
    10921 agctgttgaa ggtggcgtag acatcaaaga attaaatgtt gggtcaatgg cgcattcggt
    10981 cggtaaagtc gttgtaagta aagtattgtc tatgggacaa gaagacgttg aagcgtttga
    11041 gaagcttgaa caaaaaggtg tgaaattcga tgtacgtaaa gtacctaatg attcacgcga
    11101 taacatggat gatattttga aaaaagcaaa agctgaatta gcaaaagcgt aataaataat
    11161 attaaaattt ataggaggct tatcatgtct attatttcaa taattttagt cgtattagtt
    11221 gcctttctag ctggtatgga aggtattctt gatgaatttc aatttcatca accactagtg
    11281 gcatgtacat tgatcggttt agttaccggt aacctcgaag ctggtatcgt acttggcggt
    11341 acgcttcaga tgatcgcttt aggttgggca aatatcggtg ccgctgtagc accagatgct
    11401 gcattagcat cagtagcatc agcaattatt ttagttttag gtggacaagg tgttaaaggt
    11461 gttccttctg cgatcgcgat tgccgttcct ttggctgttg cagggttgtt cttaacaatg
    11521 atcgtacgta caatcgctgt tccaatcgtt catttgatgg atgcggctgc tgaagaagga
    11581 aacatccgta aggttgaaat gtggcacatc attgctgtat gtttacaagg ggttcgtatc
    11641 gcggtcccag cagcagcgtt gctatttatc ccagcacaaa ctgtgcaatc attccttgaa
    11701 tcaatgccag catggttaac agatggtatg gctatcggtg ggggaatggt cgtagctgta
    11761 ggttacgctt tggtaatcaa catgatggct acaaaagaag tatggccatt cttcgttatt
    11821 ggtttcgttg tagctgcaat ttctcaatta acattgattg ctctaggtgc tttaggtgtt
    11881 gcattagctc ttatttactt gaacctttct aaaatgggtg gttcatcaaa taatggaggc
    11941 ggaagcaact ctggtgaccc tttaggcgac attttgaatg actattaatc ttgaaggagg
    12001 aaaataaaat ggcagaagaa aaaatcaaat tatcaaaaaa agatcgttta gctgttgcgt
    12061 ggcgctctac cttcattcaa ggttcatgga actacgaacg tatgcaaaac ggtggctggg
    12121 cattttctat gattccagca atcaaaaaat tatacaaaac aaaagaagaa cgttctgcag
    12181 cattaaaacg tcacttggaa ttctttaata cacacccata cattgcttct ccaattttag
    12241 gggttacatt ggctctggaa gaagaacgtg cgaatggcgc gcctgttgat gatgtagcga
    12301 ttcaaggggt taaagtaggt atgatgggtc ctttagctgg tgttggtgac ccagtgttct
    12361 ggtttacagt acgtccaatg ctaggtgcac taggtgcttc attagctatg ggaggaaata
    12421 tccttggacc aatcatcttc ttcttaggtt ggaacattat tcgttgggca ttcatgtggt
    12481 atacacaaga atttggctac aaagctggtt ctaaaattac tgatgacctt tctggcggat
    12541 tgttacaaga cgtaacaaaa ggtgcttcaa ttttagggat gttcgtatta gctgcccttg
    12601 tacaaagatg ggtatcaatc aaattcttac cagttgtatc aactgtaaaa ttagataaag
    12661 gtgcttacat cgaatgggat aaattgcctt caggtggtga aggaatcaag agtgcatttg
    12721 aacaagttaa taatggctta gccctttctc ctgaaaaggt aacaacatta caagacaact
    12781 tagaccaatt gattcctggt ttagctgcat taggattaac attgttatgt atgtggttgc
    12841 ttaagaaaaa ggtttctcca atcgttatca tccttggttt attcgtagtc ggtgttgttg
    12901 gacacgtcat cggactttta taatccataa aaatatagct cgggcgttgt cccgagcttt
    12961 ttttatatag tatactgttc ataaacaaat atctggaggg agaaaatggt tcaatcaatc
    13021 aatacaaaag tagacttggt aattgatgct acttcgttta cgggtctgac agattacggg
    13081 caaatcatga taggtaataa tggttttgag ttttaccatg cgcgagatag acgcaaatat
    13141 atccaaattc catgggaaga agtggattat gtactagcat cagtcatgct aaagggaaaa
    13201 tggattcctc gatatgccat tcaaacgaaa aaaaatggta catacacatt ttcttccaaa
    13261 gaacctaaaa aagtgcttcg tgcgatcaga gaatatgtag atgaagatag aatggtacaa
    13321 tcattgagtt tctttgatgt agtgaaacgt ggtattcgta gtatgtttaa acgcaagtaa
    13381 aaaataaaga aaacttctaa gaggaggttt tctttatttt tttactttgc agaagaaaat
    13441 atgaaatagc aaaaccaatt atttgtcccc tttaaatccc atatgctaaa attgattagc
    13501 aaagaaggag gttatattat gagttggtta tggacattga ttgttggagc cgtgatcgga
    13561 gcaattgcag gtgcgatcac cagtaaagga aattcgatgg gctgtgtaac gaatatcatt
    13621 gctggactag tcggttcaag tattggacaa gcgattttcg gcgaatgggg accaaaagct
    13681 gcaggtatgg cacttattcc atctatttta ggcgcagtga ttttggtagc agttgtctca
    13741 tttttctttg gaaaacgatc ataagttttt acaaaatagt aaactaaaag gagttgcagc
    13801 gagactgtga ctcctttttg gctttattta tttaaaataa aaagaagttt tccggaaatt
    13861 tttatttaac tgtcgtgtac gaagaaaagt ttgctataat ggaaaaaagc gtgcaaagaa
    13921 agagggagtt tcattatgat gctacgtgta gaaaaattgc gcaaaaaaat gcaagaagaa
    13981 aatttggatt catttttggt taccagtccg tacaatcttc gttacttaac gaactttaca
    14041 ggtacgacag gattagctgt tattactttg gaaaaagcat tttttattac tgattttcgt
    14101 tataccgagc aggcggcagc gcaggctcaa gggtttgaaa ttattaaaaa tgtcggacct
    14161 atttttgatg aggtagcaaa cttagtacgc aaagaagaac tgcactcatt agcttttgag
    14221 gaaacaacag tctcattttt agaatattcg gttttagaag aaatcatcga tgcggaatta
    14281 gtacctgttt cacagatgat cgaagaactc agagaagtga aagatgaaga agaaatcgct
    14341 attatcgaaa aagcatgtca tattgcggat atggcatatg atcacatcct gaaaatgatc
    14401 caaccgggca tgactgagat tgaagtcgca aatcaactag acttttatat gcgttctctt
    14461 ggcgcttcag gtgtttcttt tgatacgatc gttgctagcg gattgcgttc ggctatgccg
    14521 catggtgtag caagcaaaaa aatcatcgaa caaggtgact tgatcacatt ggactttggt
    14581 tgctattacg aagggtacgt ttccgatatg acacgtacat tcgctattgg tgatcctggg
    14641 gaaaaattaa aagaaattta tcaaatagtc ttagatgctc aattagcagt tattgatgct
    14701 gcaaaaccag gaatgactgg tgtccaatta gatgcagttg ctagagatta tatttcaaaa
    14761 catggttacg gggaagcatt tggacattct acaggacatg ggatcggttt agagatccac
    14821 gaaggaccaa acgtttcttc tcgtgcagaa aagcaatttg taccaggaaa tgtgatcact
    14881 gatgaaccag ggatctactt accaggaatc ggtggagtac gtattgagga tgacttgttg
    14941 atcacagaag atggaaatcg tgtgttgact cgttcaccaa aagaattgat catcttatag
    15001 tttcatcaat agaagaagta agcgtaaaaa tcagtgttca cagactttgt tatagaaaat
    15061 cactcgattt aatgatacac tatagaagaa cttttaagct gtaagagata ataaagttgg
    15121 gtgttttctc caattcatag cagtttgcag ctgatcggcg gcagttttct taccacgtga
    15181 tcactatgcc gcatcataga gaattcggat gtatctcgtg cttaggcaaa gggaggaata
    15241 aataaatggc agatgaaaaa aatttgattt taaacgcaaa ccaagattta ggagagatcg
    15301 tgatcgctcc tgaagtcatc gaagtaatta tcgggattgc agcttctaaa gtagaaggcg
    15361 tatacggaat gcgcggaact tttgccagca atgtcactga attattagga cgtgcagctc
    15421 acggaaaagg tgtttactta gcaaacgagg aagatggctt gaaagtagat ctttactgtt
    15481 atctagaata tggtgtttca gttcctaaag tagcaatgga aatgcaggaa cgggtcagcc
    15541 agcaagtcct tttcatgacg gatatcgatt tatctgaagt caatatccat gtagtagctg
    15601 ttgttcctga aaaattagaa caaccaacaa tcgaagaact ttttgaagac gaagaggaag
    15661 agaatgaata aagaattatc aagacacgag attcgggaaa tggcattgca ggctttattt
    15721 ccattagact tcaatgcaga cctaacaaaa gaagatgcga ttttcaacgc aatcgaatta
    15781 gaccaccgcg acatgatcaa cgaagatgaa tcagagtttg ttcctgttta tcttgatacg
    15841 ctagttggcg gtgtctgtgc aaaaaaagat gaattggata aagtgattga aaaacattta
    15901 aaaaacaatt ggcacatcaa tcggatctca aaaatggatt tggtgatctt gcgtatcgcg
    15961 atttttgaaa tgacttatgt gacagatgta ccagcggctg ttgctttaaa tgaagcaatc
    16021 gagttggcaa aaacatttag tgatgaccgt tcacgtaaat tcgtcaacgg tgttttatcc
    16081 aatgtattga aagaattaga agctggggcg taagtcctag cttttttcta tgaatcatca
    16141 taaacctgtt ataaatcggg aaaattctat ggtagaatga aaacacaaaa aatatctggg
    16201 agtggttaga tggcagaatt gattaacgga aaagaattag ctgaaaaaat gcaggcagag
    16261 attgctgaaa aaatccaaaa attgaaagat aatggtatcc atcccggatt agtcgtttta
    16321 ttggtagggg aaaatccagc cagccaaatc tatgtaagaa ataaagaacg tgtagcaaaa
    16381 gaaatcggaa tccattcatt ggtagagcgt tatcctgaga cgatttctga agacgaacta
    16441 cttgcagaaa ttgaaaaata caatcaagat cctagttttc atggtatatt ggtccaatta
    16501 ccattgccaa agcatataga tgaagaaaag atcctgttag ctatccgccc tgaaaaagac
    16561 gtagacggtt ttcatccgat gaatttaggc agactccttg cgggtaaacc ggacaagatt
    16621 ccttgtactc catacggcat catgaaaatg tttgaagctt attctatcga tccggcgggt
    16681 aaacgagcag tagttatcgg acgaagcaat atcgtcggta aaccgatggc tcagctactc
    16741 ttgatggcag atgcaactgt tactgttgct cattctaaaa ccgaaaatct ggctgaactg
    16801 gcacaagaag cagatatttt agtcgtagca ataggacgtg gacattttgt cacaaaagaa
    16861 ttcgtaaaac caggtgctgt agtgatagat gtagggatga atcgtgatgc taacggtaag
    16921 ctgatcggtg atgtgaaatt tgatgaagtg gagcctattg caggctggat cactcctgtg
    16981 ccaaagggtg tagggccaat gactatcacg atgctgatgt atcaaaccgt aaaatctgcc
    17041 gaagcttttg gagcaggtga atagatggca caagaatatt taactgtcac tacattaacg
    17101 aagtatttga aaagaaaatt tgatgccgat ccttatttag aacgcgtata tttaacaggc
    17161 gaaatatcga atttccgttt acgtgccaat gcccatcaat atttcagctt aaaagatgac
    17221 catgcaaaaa tatctgctat tatgttcaaa ggagcattcc aaaaattacg attccagcca
    17281 aaagaaggaa tgaaagtcct tgtcgttggg cgtatttcat tatatgaagc gggaggttct
    17341 tatcaaatct atgtagagca catggagcct gatggaatag gtgctttata tcaagaattg
    17401 gaagaacgaa aagaaaagct gatgaaggaa ggcctttttc aagggcctaa aaaaatgctg
    17461 ccacgtttcc caaagcgaat cgcggtcctt accagtccaa gcggagcggt tatccgcgat
    17521 atcatcacga cggtgaaaag aagatatcca attgtccaat tggttttgtt cccaacagtt
    17581 gtacaaggaa accaagcagc ggacaatgtt gtcaggaata tccagagagt agaagagatg
    17641 ggcaatttcg atacaatgat catcggtcgt gggggtggtt cgattgagga tttatggccg
    17701 tttaatgaag agcgggttgc tcgggcgatt tatcaagcta atacgccggt gatttcatct
    17761 gtaggacatg aaacagatac cacgatcgct gatttagtag cagatgttcg ggctgccact
    17821 ccaacagcag ctgcagaatt agctgttcct gttttgtcag aagagttgat gcgcattcaa
    17881 gaacggcaag ctagattgca gcaggcgttt gtacgccaga tccagcgcaa acaagaacgg
    17941 ttcgagcgtg ctcgacgttc ctatgtattt cggcagcctg aaagattgta tgaaggtcag
    18001 accatcaaat tagaccagtt gaaacagcga ttatatcaag ctacacagca aatttatcat
    18061 gcgaaagaaa aacaaacgat cgcattaagc caccaattgg aacaagtggc tccgctttat
    18121 cgagttaaag cagctcgcca agaaactgct tatctagaaa aacgcttaga agagaaaatc
    18181 caacaatata tgaaacaaca gcagcagcgt tttgctcaag ctgttcaatc tcttgatttg
    18241 ttgagtccat tgaaaatcat gggaagaggc tacagctata cgacgttaaa tgatcaagta
    18301 gtaaaaagtg tcaccgagat tcatcaaggg gatttgctga cgatccatta tcaagatgga
    18361 atcgttaaag gacaagtaca agagatagac aaaggagaat aaaaatatgg aaaatccaac
    18421 ttttgaagag tcactgcaag aattagaaaa aattgtcatg cagttagaac aaggagacgt
    18481 tccattagaa tcagctctag attcgtttaa acgaggaatg gaattaagca aacattgcca
    18541 agatacatta gctaaagcag aagagacctt gacgaaaatg atgacagaat caaatgaaga
    18601 agttgatttt gacggaaacg aggaaagctg agatgagatt aactgacttt tcagctattc
    18661 atctacctgc tgtagaaaaa gaaatcttgt cttttttaga tgagcataca acggatagag
    18721 gactctatga tgcgatgagt tattcagtga aagcaggcgg gaaaagaata cgtcccttat
    18781 tacttttaac ggccgttgct tcctttgatg agcctattga tgttcccgtc tatcaagtag
    18841 cagctgcttt agaaatggtg catacgtatt ccttgatcca cgatgatttg cctgcgatgg
    18901 acaatgatga tcttcgtaga ggaaaaccta ccaatcataa agtattcggc gaagcattag
    18961 ctatattggc cggagatggt ttgcttacag gagcattcca gctaatcagt atggcacact
    19021 taggtaattc tcctaagctt ctgctgcttc agcaattagc tgtttgtgcg ggatcacaag
    19081 gtatggttgc tgggcaagct gcagatatcg agggcgaatc gaaaaagctt tctttagaag
    19141 aattagcatt tattcatgaa agaaagacag ggcatttgat tcgttatgcc ttgctggctg
    19201 gtggcatatt agcgaagcaa cctgaagaaa tcttattgct acttcaacga ttagccgaac
    19261 atttaggttt agcttttcag ataagagatg atctactcga tgtgattggt acaacgaaaa
    19321 cattagggaa aacagctggt aaagatgaac gtatggaaaa aaatacttac ccgcgtttat
    19381 taggcctaga aaagacacgt gaggcgttgg agattgagct gttatccgcc aacaagatca
    19441 tagacaaatt agaagaggac gttttttctt ttgatggcgg gctgatgcgc cagatgatca
    19501 agcaatttga tgtggagaaa tgagggaaag aatggaaaaa gaacgcgtag atgtattagc
    19561 ggtcaaacaa ggattatttg aaacaagaga acaagccaaa cgttcagtga tggctggttt
    19621 gatttacaac gaaaaaaacg agcggtttga taaaccggga gaaaaaattc cagtgagtag
    19681 tgaattaaga gtcaaaggta aaaaacttcc ttatgtttct cgagggggat taaaattaga
    19741 aaaagcactc aaagcatttg atctatctgt tgaaggaaaa acattgttag atattggtgc
    19801 atccactggc ggatttactg atgcagcttt gcaaaatggc gcaaaaatga gttatgcttt
    19861 agacgttggc tacaatcagc tagcttggaa aataagacaa gatcctagag tcgtcgtaat
    19921 ggaaagagtg aattttcgtt atgcaaaacc cgaagacttt accttaggaa ttccagaagt
    19981 tgctgttatc gatgtttctt ttatttcact aaaactgatg ctgcctccat tacatgctat
    20041 tttaaaagaa gagggagaag tgattgcttt aatcaaaccc caattcgaag caggcagaga
    20101 agctgtaggg aaaaacggaa ttgttcgtga ccctcagacc catcaaaaag ttttagaaga
    20161 cattttgtca tttgcggcac aaggtggata tgatgtgctg gaattaagtt attctccgat
    20221 cacgggggga gaaggaaata tcgagttctt ggctcacttg aggaaagtac ccgaaagcgg
    20281 aacaatcaat tcagctatta atatggcaga agtggtttca aatgcacacg aacagtttga
    20341 ccataaataa attgtaactg tgaaaatcaa gtgagaagta tgcggattct tctttattat
    20401 accaaggaaa taagctatca ttgattaaga agaatatgct aaggagcgaa gacgtaatga
    20461 gaaaaaaaga tcggcatcgt ttgattactc gattgttgag tgagcaagat atccgaaagc
    20521 aggaagaatt tgttgaaatc ctgaaaagca aaggaatatc tgttactcaa gctacgattt
    20581 ccagagatat aaaggaactg aaactgatca aggtaccggc ttcagatggc ggctaccgtt
    20641 atagcatacc agctgaaaca aacgaagatg taaatactaa attggaaaaa ctattaaaag
    20701 acgctttcgt ggcagttgac caaatggaaa aatttgttat tttgaaaaca ttgccgggaa
    20761 atgcttcggc agcagcaaat ttgattgata aacgttacaa gaaagaatta ttttcgatta
    20821 tcaatgatga cgataatgtt ttgatgatta cacgaactga agaccaggca gttttattga
    20881 agaaagactt cctgcattac ctttaagttg aggagcgaat gtgatgctac aagaaatcag
    20941 tattactaac tttgcgatta ttccggaatt gcggctttct tttcacgaag gaatgaccgc
    21001 gctgaccgga gagactggtg cgggtaaatc cattattatc gatgctttag gtctgttagc
    21061 aggaggaaga ggttcaagcg attatatacg tcaaggagca gaaaaatgtg tgcttgaagg
    21121 gttgtttgaa ctaccaaaac aagaaggatt ctctgaactg atggttgaat tagggatcga
    21181 aacagatgag gacaatctga ttgttcgacg ggatatgtcc ttaactggaa aaaatgtttg
    21241 tcgagtgaat ggtcatatta tcacattagc taatctgaga agaatcggca gttatttagt
    21301 agatatccaa ggacaaaacg aacatcagga actgctccag cctgaatcac atcttgcttt
    21361 attggatcgt tttggagatg tagctttcca acagaagaaa aagagttatc agcaagaata
    21421 tatctcttat cgtgagctgg aaaaaagggt acgaaaagtg cagcaaaatg aaaaatccta
    21481 tgttcagaga atagatatgc tccatttcca gcaagaagaa atagctgctg ctgatttgca
    21541 agtcggagaa gaagagaaat taaaagaaga aagagaaaaa cttagcaatt atcaaaaaat
    21601 tgctgatgga ttagctgcag gttacggagc actgacgaat aatgaacaaa acagtgtaga
    21661 tggtgttggc ttggccgttt cagaactgca agggatcgcc catctagatg tggagtatga
    21721 agcaatttat gaaaatatcc aaagtgctta ctatttgctg caagatgcaa tcggcgatat
    21781 gagtcgtcag attgatttat tggagttaga tgagagtcgt ttagaagaag ttacgcaacg
    21841 tttagaatta atccgacagt tgaaacgtaa atacggcgaa tccattgagt caatattggc
    21901 ttactatgac gaaatcaccg aagagctagc ttcttctgat ttttcagaag gtcaattgga
    21961 caaaatgaaa gaggaactag agcaaaaaga gcttctactt cagcaacaag ccgcagatct
    22021 tcatgaagcg cgtaaaaaaa tcgcaaaaga attggaaaaa tccattctgc atgaattgaa
    22081 aagtctgtat atggaaaaca ctgagtttga ggtacgtttc ttaaaagaag aaaatagaca
    22141 attgaatcat gatggatttg accaaatcga attttacatt acgaccaatc caggagagcc
    22201 actgaagcct ttggtgaaag tcgcttctgg tggcgaactg tctcggatgt tgcttgcttt
    22261 gaaaactatt ttttcttctg agcaaggtgt gacaagtatt atttttgatg aagtggatac
    22321 aggtgtgagc ggaagagttg cacaggccat cgcagataaa atttctcaaa tatcgaaata
    22381 ctctcaagtt ttgtgcatca ctcatctgcc tcaagttgct gcagtcgccg actatcaata
    22441 ttatattgtg aaagcagtga ttgatggacg tacgcaaact tctgtttcag agttgaaaac
    22501 aaaagaaaga gaagaagaaa ttgcacgtat gctggctgga agtgagatta caaaactcac
    22561 actcgaacat gcaaaagaac tattgaaact cgcaaaaagg acctgagcca cctcgcagtg
    22621 gttcaggtcc ttttcagcag ctgatagcta tattttattt ttacatgaca ctcattacag
    22681 cagtcaatac aacagatcca atcgttaaaa tcagcattgt ccaaatgaca aattttgtta
    22741 ctttagcaaa agtacttgtt tgtttttttt cacgcattgt tttcaacctc gtcattaatt
    22801 agttgattca agtgtacaat cttttttcca tttccacaag agggcttttt tataaaaact
    22861 tccctttttt taaataatag gtagttacga tacaaataaa taatgttaat agaactagcc
    22921 atataaaggc atcatccctt tcagcaatag gtaacttaat gttcattcca tagatactgc
    22981 cgacgattgt cggtatagtt aaaataatcg ttaaagaagt taaaattttc ataatattat
    23041 tcagattatt cgatacgatt gctgaaaaag tctgattcat ttgttctact agttttaatt
    23101 gaatgcgaga agtagtcatt gcctgctctg tttccaccaa tacatcacgt aaatgatttt
    23161 tcaaagcatg attttcttgg aaaagtttat tttgcgctag ttctttcaat gcatctaaat
    23221 tagcagaagt cgcttctctg aaataaacga ggcttttttg gatatccatg atttgataaa
    23281 gttgtttgtt ttcagtggat acttgaagtt tggcttccaa agtatcgcat tgagattgaa
    23341 gcgtagctaa ataactgttg taagagagtg tgacctgcca aatgaattga aagaagatat
    23401 tgagattagg cgaaatatca ctttttggaa aggtctggct aaagactttc tttaaaaaca
    23461 gtggttcatg attcgtaatt gtgataagct tctttttggg cgtcaaaata atagagagcg
    23521 ggtatgtatc cacttgaaaa taaccgctag gacttgtttt agcaaaagga tattgtaaca
    23581 ataataacgc tggctttgtc aactttgatt gattgaattc ttctgtacgg gaattttctg
    23641 catcgtccaa tatggaagtg atgtaatctg acggaatttc aaattgatgt gtcagctgat
    23701 gaatctcttc ttgattagga cgttctacac aataccagat cgtttggtca gtttcctttc
    23761 cggtattacg cagtttctca ttatctaatg taaaatattt gatcatgatt ttcctcctgt
    23821 tgcctgtttc ttttattata acggataggg aattggaata catggaatgt gtttgtaaga
    23881 tatagaatat aactgcataa atttaaataa aatgaaacag ttgtgtaaag tatatacagt
    23941 atacttgaag caacgataaa aaaggagttt acgttcatgg gcacattcac accacttatc
    24001 ttgttttggt atatttaccc ggtaatcgtt ttgtttggtt gccaatttct tgtaaaatta
    24061 ttttcgttaa atcgtcgttt taaaataaaa gcccctgatt tggcgattcc ttttttgtgg
    24121 ggcggtatgc atgcattgtc tagaaataca gggacaattt ctatcttacc gtttttcttt
    24181 atttctgttt tgctaatggg aatttgtatt gctgttttcc aagcatatta ttatgaagaa
    24241 attatttatc cgcgttattt caagatgact tggcgtttgg tttttctgtt gacaatcgtc
    24301 ttgtacgctg tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa atagaaaagc
    24361 tgtaaaaagt cttttaaaag gctttttgca gcttgatttt taaaaaaatg tttatgaata
    24421 aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa agtgggggat tgtggtagac
    24481 taattttagt tagtggagga atggggggct ttcaatgttg atgggtgaat tccaacataa
    24541 tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac ttggcgaaaa
    24601 gtttgttctg acacgcggat tggatggctg tcttttcggc tatccgatga gcgaatggga
    24661 aaatctggaa gctaaactga acgaaatgcc gttggctaaa aaagacgcac gtacttttgt
    24721 gcgattcttc tattcggctg ccactgaatg cgaacttgat aaacaaggac gaatcaatat
    24781 acccagcacg ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca tcggtgtatc
    24841 taaccgaatt gaaatttggg atgagacacg atggcaagat ttttctgcag cagcagaaga
    24901 gaactttgac gaaattgctg aaagtatgat tgatttcgga ctataagaat aggaagaggc
    24961 tgactaatga ctgaaacatt tcaacactat acagtcatgt tgaaagaaac ggtagatgga
    25021 ttgaacatca aaccggatgg cgtttatgta gactgtacac tcggcggtgc cggacatagt
    25081 gaatatttat taagccaact aagttctaaa gggcatctgt atgcgtttga ccaagatcaa
    25141 aaagcaatcg atcacgccaa gctgcgttta gcttcttatg tcgaacaagg tatggtcaca
    25201 tttatcaaag cgaacttcag agaattagaa gaacgcttga gtgagcatgt agataaggta
    25261 gacggcattt tgtatgatct aggggtttcc tctcctcagc tagatgaagc agaacgtgga
    25321 tttagttatc atcaagatgc accgttggat atgcgcatgg atcagtcagc tcctttatcc
    25381 gcttatcacg taattaatga atattcctac catgaattag taaagatttt cttccgctat
    25441 ggcgaggaga aattttccaa acaaattgct cgtgaaatcg aacgagtacg aaaaacaaaa
    25501 ccaatccaaa caactggtga gcttgtcgaa atcattaaac aagtcatccc agctcccgca
    25561 agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat tgcagtaaat
    25621 gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa tccggttatt gaaagtcaat
    25681 ggaagaatca gcgtaatcac attccattca ttggaagaca gaattgtcaa aagtatgttt
    25741 aaagaatata gtacggtaca agatctacca ccgggacttc ctgtcatacc tgacgaattc
    25801 caaccagaac tgaaagtcat tactcgtaaa ccaatccttc cttcagagga agagctaaca
    25861 gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag aaaaaataag aatgaaagag
    25921 gagtagaatg atggcagaat taaagaaaat gcaagattac gaatatgatt tacctgaatt
    25981 ggtggatcag ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa tcattcttcc
    26041 acaatcaccg gcaaaacgat taaagcagat ttctgctttg gaaaaaattg ctgtggcaag
    26101 cgtggcgatt gctattgttg ctttatgtgt tttaacagtt atgctgcgca caaatatcag
    26161 cggagctgaa aaagaaatca cgtcaataca aacagaaatc aatgataaaa agcaagaaaa
    26221 aacgagttta gaacaagaaa aaaacgaact ttctcgtact gaacggatta aacaaatcgc
    26281 agaagaaaaa ggattgtcta ttaatgacga caatttaagg aaagtgaagt aaatgagtct
    26341 taaaaataaa attcggcggt ttatggaaaa gaaaaatcta aatccgatga acaatcgcaa
    26401 aaaagtaggt atcattttat ttgctacgag tattggattg ttctttttat ttgccgtccg
    26461 tttctcgtat atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa
    26521 acagttgtac caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa
    26581 cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac
    26641 atatacctct ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga
    26701 aatcctgcat aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg
    26761 cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga
    26821 aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt
    26881 cgaccatcag gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc
    26941 agttccagac aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa
    27001 tgatatcttg agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc
    27061 tctgccaggt acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac
    27121 tttagacagc agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata
    27181 tcagccagaa gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat
    27241 gggacaacga cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat
    27301 ctggcgtaat ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac
    27361 tactgcagct gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa
    27421 aatccaagta gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac
    27481 catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt
    27541 aggcggacga tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg
    27601 attagatgat gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag
    27661 tgcttatgga caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat
    27721 tgcgaataac ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac
    27781 tggagaagag cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac
    27841 agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtagtgctta
    27901 tggtgtatat agtgtacctg gatacaatgt atcggcaaaa aacggaacag cccaaatcgc
    27961 ttctgataca gggggatatc aaacaggaga tacagcctac ctgtattcga ttgtggagat
    28021 ggttccttct gaagatccag actatgtctt gtatctaaca atgaaacacc ctaagacata
    28081 tgatcgaatg gcattggcta aaatcgcaaa tccattgatg aaacgggcaa tggattttaa
    28141 agaaactgaa gaagattcag atacagaaac aaaaacagaa aaagtatccg tagccgatta
    28201 tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac tgcaacctgt
    28261 agtgatcggt aatggtaaaa aagtacaaaa acaatctact gccaacggtg atcagttgat
    28321 ctctggagaa aaactgatcc tatatactgg tggtgataag ctgatgcctg acgtaacagg
    28381 atggtcaaaa gcagatatca tgaagctggg gaaaatcctc ggtattgaag ttagttttga
    28441 tggagatggt tattgtgtca aacaagaact tgctccatat gaaaaaatca ctgaagacaa
    28501 acttagtttt actttagaag aataagaaga gaaataggag agaaaagagc atggaatgga
    28561 cacaagcatt gattcccatt gtcagcagtt gtgcaatgac aattgccgca atgccgctat
    28621 ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag ggaccaaagt
    28681 ggcacaatag caaagccgga actcctacaa tgggcggctt agtgtttctg atcggctcta
    28741 ttttgacagg aatttgggtt ggagcttggc aaaaacagct aactcctact cttttcatct
    28801 tgctttttgt attagcgtta tacggagtaa tcggctttct tgatgatttt ataaaaatat
    28861 tcaagaaaag aaacatggga ctgaactcga agcagaaatt gattggacag attctagggg
    28921 gaatcatctt ttatcttgtt taccgttctg aaggttatcc aggtgtattg aatttcttcg
    28981 gactagatct acctttagga atcatttatg gtattttcgc tctcttctgg cttgttggat
    29041 tttcaaatgc agtaaatctg acagatggca tcgatggatt agttgctggc ttaggaagta
    29101 tctcttttgc agcttacggt ctaattgctt ggcatcagca tcagtatgat gtattaatca
    29161 tttgcttaag tgttttagga ggtttactgg gtttcttcgt ctacaacaga aaacctgcca
    29221 agatttttat gggagatgtc ggttcattag cgttgggcgg acttttagcg gcaatttcta
    29281 taatgctaaa ccaagaatgg acattgcttt tgattggttt gatctatgtt atggaaacag
    29341 caagtgtgat gcttcaagtt acttccttta agcttacagg aaaacgaatt tttaaaatgt
    29401 ctccaattca tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt gatacagttt
    29461 tttggttgat cagcattgtg acttcgttga tcacattatg gatcgtttgg taggagatga
    29521 cgggaaatga aaaaaatatc aacatatgaa aataagaaag tgcttgtctt aggactcgca
    29581 aaaagcggag taagtgcggc caaattatta catgaattgg gcgcacttgt caccgttaat
    29641 gatggaaaac cttttgacga aaatccggaa gcacaagagc tgctttcttt agggataaaa
    29701 gtaataacag gtagtcatcc aatcgaattg ttggatgaag aattttcatt gatggtcaaa
    29761 aatccaggga ttccctacag tcatccttta gtagcaaaag cccaagaaat gggtattccg
    29821 gtaattacgg aagttgagct agcttacgaa gtagcggaat gcccaattat tgggattaca
    29881 ggaacgaacg gaaaaacaac gacaacaacg atgacaggac tactactgaa tgcaggagca
    29941 gatcaaggaa ttgcacgttt agcgggtaat attgggtatc cagcaagtgg tgtagcgcaa
    30001 gaagcaaaat cagaagataa aattgtgatg gaattgtcta gcttccaact catgggaatc
    30061 acagatttcc gccctcatat agcggtgatt accaatatct atgaagcaca tattgattat
    30121 catggtacaa gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa catgacagaa
    30181 aaagattatc tgatccttaa ctggaatcag tcagaactac aagaattagc gcaaagaaca
    30241 aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt gtatgctgac
    30301 gattactcaa tatactataa aaaagaaaaa ataatggaaa tatctgaact tggcgtacca
    30361 ggtaagcata atgtagaaaa tgcactagct gctatttctg tagcaaaatt gtatggtata
    30421 tcaaatgaag ctatacgaga aacgttgcat tttttccatg gcgtaccaca tcgtacgcaa
    30481 tatgtaggag aaatccaagg cagaaaattt tataatgatt caaaagcaac caatatttta
    30541 gcgactaaaa tggctctaag tggatttgaa acgtctaagg tcgtgttatt ggctggcgga
    30601 ttagacagag gaaatacatt tgatgaatta attccttcat taaaaggaat caaagcaatg
    30661 gtcgtatttg gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc aggcatcgag
    30721 acaatagtaa cagccgattc tgtagaacaa gcagttcctt tagcattaga aaacagtaca
    30781 gatggtgatg tagttttatt atcaccagcg aatgccagct gggatcaata cccaaatttt
    30841 gaaactagag gaaacagatt catggaagct gtgaaccgat taaaatagaa aaagtagaag
    30901 cgagcgataa aaaatgaaaa ttttggttac aggcggagga acagggggac atatttatcc
    30961 agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat tcatgtatgt
    31021 gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat acaggaatgc ctttccatac
    31081 cttggagatt caaggattta aacgaaaaat ttccatgcat aacatcaaga cggtccgatt
    31141 gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt gattttcaac cagatatcgt
    31201 tatcggtact ggaggatatg tttccggagc ggtagtgtac gctgcagcca aaatgggtat
    31261 tccaacagtt atccatgaac aaaacagtgt accaggtgtt accaataaat ttttaagccg
    31321 atatgtggat cggatcgctc tttcatttaa agatgcggct tccttttttc ctgaaaacaa
    31381 agcagttctt gtaggtaatc ctcgagcaca agaagtagca gatactaaaa aatcagatat
    31441 tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc ttgatttttg gcggtagtca
    31501 aggagcactt aaaatcaatc aagcagtatc agttttttta agtgaatata ttccttcttc
    31561 ttaccaagtt ttgtatgctt caggagatag atattatgaa gagatcctaa ataaaataaa
    31621 tcctgaaata caaaatatta gtgtccagcc ttatatcaaa aatatggcag aggtcatggc
    31681 ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat tcacagctct
    31741 ggggttaccg gcagtgttgg ttcccagccc atatgttacg aatgatcatc agactaaaaa
    31801 tgcaatgagt cttgttcacg caggagcagc aaaaatgatt gcagataatg aactaacagg
    31861 tgaatcacta agtcagacag tcaacgaaat catgggtgat gaagaattgc aaaagcaaat
    31921 gtgccgagct tctaaagaac aaggaatccc tgatgcttca aaacgtcttt atgacttagt
    31981 aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa agaaccaaaa
    32041 aaattgtctc atccgtcttc tgatgaaaac aatttgactc cttggcaaaa ggctaaccga
    32101 aaatatctgg cagaacaaga gatgaaaaac caagagccaa aaaaagaaga cataacatta
    32161 gatacacccc tttcagattc tgaagaagaa aaaacggttc aagaggaaca gcaagaagaa
    32221 agccagtccg agaaaaaaat cgaatttttt gaagagatca agcaaaagaa aaacggtgga
    32281 ccatataacg gttctttttt gaaccggctg cccaatttaa aaagtcaacg gcacaaagta
    32341 ctatttcgtc ggttagcttt gatcatcact gtattgggta taccgctgat ttttttgatc
    32401 tattatgttt ctccttatag caaattacaa gcagttgcag tatcaggcaa taagacagtc
    32461 aacagccaag aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt ttggggacag
    32521 tattttcatc gatctactta tatcgatcgc cttaaaaaag cacagccaag aatcgaaaca
    32581 gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga atataaagag
    32641 attgctttga tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg aacagtatta
    32701 gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct tggaagagtt caaagactct
    32761 gcaaaaatca aagaattaac aaagcaatac aatcagttat catcagaatt acaaaaagca
    32821 atttctgaaa tcaagtacac gccaagagca agcaataaaa atctgatcca attgaatatg
    32881 aatgacggca atcaagttat tgtaaatatc aataatcttg cgaaccaaat gaaatattat
    32941 tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt tggtatcttc
    33001 tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg gccaatcgga agaaacaact
    33061 gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga aacgcaaaaa
    33121 gcacaaacac ctggatcgtc tagcacaaac ggatcatctg attcaaatga ttcaagtgaa
    33181 acaagtcaaa tagaagataa tccaagtaca actccgtcaa gtttaaatga aaattgataa
    33241 aacaacttta aaaagtaaga atgtactttc taaaaaccag taatttatgg tagaattgga
    33301 agcagtgaat atatctatta ataagtatat tttaaaacgg acgctagttg ttcgcttgta
    33361 tatggaatag taagtaggag ggaatcccat tcatggcaaa aacaggaatg tatgtaggcc
    33421 ttgatattgg aacgacatct gttaaagttg tcgtggctga atatatcgac agccaaatga
    33481 atattattgg agtaggaaac gcaaaatcag aagggattaa ccgaggcatt atcgttgata
    33541 tcgataaaac ggtccaagcg atacaacgag cagtacgaca agcagaagaa aaagcaggta
    33601 tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta gaaaattgcc
    33661 agggaatgat tgcagttaac ggagattcaa aggaaatcac cgatgaagat gtcagaaatg
    33721 tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg tcaatattgc
    33781 cacaagattt tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga atgatcggcg
    33841 taagattaga aatgtacggt ctgttgttta caggacctaa aacgatcatc cataacatac
    33901 gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact cctttagcgc
    33961 tgactgaatc aattctttct gacggagaaa aagattttgg tacgatcgtc attgatatgg
    34021 gtggcggcca aacaacaacg gctgttatgc atgataagca attgaagttt accaatcttg
    34081 accaagaagg tggcgaattt gtcactaaag atatctctat tgtattaaat acgtcattta
    34141 acaatgcaga agcgttgaaa ataaactacg gggatgcata cccagaacgg acatcaccag
    34201 atgaagaatt tccagtagat gtgattggtc agtcagaacc agtgaaagtc gatgaacgct
    34261 acttgtcaga aattatttca gctcgaatgg aacagatttt caacaaagca aaagatgcat
    34321 tggaccaaat cgaagcatta gagttgccag gtggcgtcgt attgacaggc ggagctgcta
    34381 gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta aaattgtatg
    34441 ttccaaatca catggggctg agaaacccgg tatttacaaa tgtaataagt atcgtggatt
    34501 attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg ggtgaaacct
    34561 cagcagctcg tcaaatggta gttgaacaag aaacaacaaa cacatatgag agctatgaag
    34621 cacctgaaga aacaatctac gatgagccgg aaccaaaaga gtccggagaa aatgtaaaaa
    34681 ataaaatcaa gggtttcttt acaaatattt ttgattaatt catcctggga ggaataagat
    34741 atggaattct caattgataa taatatcaat gacggcgcag taatcaaagt catcggagtc
    34801 ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa aggtgttgaa
    34861 ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga aacagttatc
    34921 caattaggcc ccaaatatac tcgtggttta ggtgctggtt ctcagccaga agttggacaa
    34981 aaagcggcag aagaaagtga acaatcctta cgcgaagctt tagacggtgc agatatgatt
    35041 ttcatcactg caggaatggg tggaggaacc ggtacaggtg ctgcaccaat cgttgcaggt
    35101 atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga cacgtccatt cacttttgaa
    35161 ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga aaatgtggat
    35221 acattattaa ttatctcaaa taaccgttta ttagaagttg tagataaaaa gacaccgatg
    35281 cttgaagctt tccgtgaagc agataatgtt ttacgacaag gtgtacaagg tatctctgat
    35341 ttgatcacag cgccaggtta cgttaacctt gactttgctg acgtgaaaac agtgatggaa
    35401 aaccaaggta cagcattgat ggggatcggt gtggctagtg gagaagatcg cgtagtagaa
    35461 gcaacgaaaa aagcaatctc ctctccattg cttgaaacat ctattgatgg agctgaacaa
    35521 gtacttctta acatcacagg cggtttggat atgacattgt tcgaagcaca agatgcttca
    35581 gatatcgtag caaatgcagc aactggcgat gtaaatatca ttctaggtac atctatcaat
    35641 gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga tgaatcaaaa
    35701 aaggaacgca agtctagccg accagctaga caagcacaaa tgcaatcacc tgctcaaaag
    35761 actgtattag atatggatca agctaaacca acttcttcag aagaagaaaa cagttttggc
    35821 gattgggata tcagacgtga acaaaatgtt cgtcctagag ttgatgattc caattttgac
    35881 aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc tcatggcgat
    35941 gatgaattaa atacgccacc gtttttccgt cgtaaaaaat aaaggagagg aagagcacat
    36001 gattgctgac aacttgcaga aaatcaagca agaaattcag gattcgtgtg ctcttgttca
    36061 tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa tctgttgaat ccgctcaagc
    36121 tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag ataaattatt
    36181 agaaaagaaa caagatttat ttgattttcc tgaaataaag tggcatttaa ttggtaattt
    36241 acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat tactttcatg cgttggacag
    36301 tttaagatta gcagaagaaa tccagaagcg gttagagaaa gaattatcct gttttattga
    36361 agtaaatgtt tctggtgagg aatcaaaaca tggaatttca ccagatgagc ttttaccgtt
    36421 tgtggaatct ttagctgcct gcacaaaaat caaggttgtt gggttgatga ctatggcgcc
    36481 taaagaagct tctgatgagc aaatccataa agtgtttggt acgctgaaag aattgcgtga
    36541 tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt gaattaagta tggggatgag
    36601 tcaagacttt caaattgcga ttgaagaagg tgcaacattt atccgtgtag gaacagcgct
    36661 attcaaagac gaataaagga ggaacaaaaa tgccgctttt aaacagagga gcaattgcta
    36721 attttttcgg attagctgat gaggaagagt acgaatacca agattatcct gaaacaacaa
    36781 aaaaggctgt acagccaact aaaaaaaata caacacagaa tcaatcaact cattctgtat
    36841 ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta acgcatgaaa
    36901 gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct acaaaaactg
    36961 tagcgaaaga aagaacagct tcaagaacct ctacacaacc taaagcaaat cttgctggaa
    37021 aaatcactgt aatggaacca agagcttatt ctgaagctat ggcgattgct aaacgaatca
    37081 ttgctggaga agcaacctta gtgaattttc atttagtaga agaaaaacaa gcgagaagaa
    37141 tcgttgattt tctaacagga acagtttatg cactagatgg agatatcaaa cgtgttggag
    37201 atgagatttt tctttgtaca ccgactggga tggagattga cagttcaacc gctcaatctt
    37261 ttgcagatgc gaatttgttt gatctgtaag aggaggaact acgatttata ctattgcact
    37321 attacttcat ttacttcaac gggcagccta tttctatagc attatcttga tggtatatgc
    37381 attgctctct tggtttccag gcgggtatca atcagggcta ggacgattat tagctaagat
    37441 ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg aggattggac ccttggattt
    37501 cacaatttta ggggcaatcc tgatcttgaa cttagccgta caaggtttag cacagatcgt
    37561 tatatggata actgtccatt tagtttattg attgaagcaa caaaatcttt attctcagaa
    37621 tagataggag gggaaaattt tggatgcaaa tgtataccag cactttcggg cagatgaacg
    37681 tacatttatc gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta
    37741 tctgaccgaa tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag
    37801 cagtgatttg cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt
    37861 gattttcccc gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat
    37921 ccattatcct agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg
    37981 aacggggatc aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt
    38041 atttattgca aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt
    38101 tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac
    38161 acaagaaaga ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa
    38221 tatttccaga cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac
    38281 agaaaataca agacctgatt ttgtacttga actattagat attatttcag tcagaggtta
    38341 tggcagagtc cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact
    38401 gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta
    38461 gatatccaaa ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat
    38521 gatttcttag atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta
    38581 gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct
    38641 ttgaaccaat caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc
    38701 aaggaatccg aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac
    38761 gctgaaaaac gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg
    38821 acagatgcaa cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa
    38881 acacgagtat tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa
    38941 agtccggaat gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa
    39001 gtgatcaaag aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca
    39061 gacacaggag tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg
    39121 atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc
    39181 gagccgatga gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga
    39241 cctgcttttc cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag
    39301 tttgcactag atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg
    39361 ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg
    39421 aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga
    39481 gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg
    39541 aacgaaggaa aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc
    39601 catttaggac attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg
    39661 tctggctatc gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa
    39721 gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata cttggaaaaa
    39781 tgtcgtgaat atgcactttc ccaagtagat aagcaacgag cagatttcaa acgcctagga
    39841 gttgcaggag attgggaaca tccttatgtt acattagatc cttcttatga agctgcacaa
    39901 gtacgcgtat ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt gaaaccaatt
    39961 tactggtctc cgtcaagtga gtcttcttta gcggaagctg aaattgaata taaagatgta
    40021 aaatctccat ctatttttgt agctttcaat gtcaaagacg gcaaagatat tcttgatgaa
    40081 gatacagctt ttgtcatttg gacgacaaca ccatggacat tgccagcaaa ccaaggtatt
    40141 gctgtaaatc caaaatttac gtatgtattg gtggaagccg atggccgtaa gtttgtcgta
    40201 gcgaaagatt tgctagaaac agtccaatca gaaatcggtt gggaaaatgt aacagttcta
    40261 aaagaatttg ctggacaaga aatggaatat atgactgcaa gtcatccatt ctatgaacgt
    40321 gaatcacttg tgattttagg cgatcatgtt actttagacg ctggtactgg attagtccat
    40381 actgctccag gacatgggga agatgactat atcgctggta gcaaatataa actatctgta
    40441 gtgtcacctg tagactcaaa aggattgttt acagaagaag cacctggatt tgaaggtatc
    40501 ttttatgaca aagcaaatcc tatgatcact gatctattaa aagaaaaagg agctttgctg
    40561 aagctagatt tctttactca tagttatcct cacgattggc ggacgaaaaa gcctgtgatc
    40621 tatcgggcaa ctccacaatg gtttgcatct atcgataaat tccgtcagaa tattttggat
    40681 gaagtagaaa aagtagagtg ggttatccca tggggtaaaa cacgtttgta taatatgatc
    40741 cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat ggggagtacc acttccgatt
    40801 ttctatgctg aaaatggaga agcaatcatc acaccagaaa caatcgaaca tgtggcaaaa
    40861 ttatttgctg aacatggatc aattatctgg tttgaaaaag aagcgaaaga acttcttcct
    40921 gaaggattta cccacccagg ttctcctaat ggcgaattta caaaagaaaa agatatcatg
    40981 gatgtctggt ttgattctgg ttcttctcat gaagctgttt tacgtcagcg accagaatta
    41041 tcattcccag cagatatgta tttagaagga tcagaccaat atcgtggctg gttcaactca
    41101 agtatcacga ctagtgtggc tatcaatggt gtagcaccat acaaatcagt tatttctcaa
    41161 ggatttgctt tagatggtga aggccgtaag atgagtaaat ctttaggaaa catcattgca
    41221 ccagaaaaag tgatcaaaca atttggtgca gatatccttc gcttatgggt atctagtgta
    41281 gattatgaag cagatgttcg tgtttctatg gatatcttaa gtcaagtggc agaagtatat
    41341 cgtaaaatca gaaatacgat gcgtttctta ttagcgaata cagaagattt cgatcctaaa
    41401 aaagatactg tttcgtacaa tgatttacgt tctgtagaca aatatatgac cgttcgattg
    41461 aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca aatataactt catgcatatt
    41521 taccgtacag ttatgaactt cttgacggtt gatctatcgt cattctacct ggattttgct
    41581 aaagatgtag tttatattga agcagaagat gattatcaac gtcgttgcat gcaaactgtt
    41641 ttctatcaaa cagctgtagc tttgacaaaa ttattgactc ctatcattcc gcatacagca
    41701 gaagaaatct ggtcattcct gaaagaagaa gaggagtacg ttcagttaag cgagtttcct
    41761 ggttatgaag agtttgcaaa ccaagaagaa ttgatggata cctggactgc tttcatggac
    41821 ttccgtgata atgtcttaaa agcattggaa gaagcaagaa acagcaaatt gattggtaaa
    41881 tctttggaag caaaactaac tgtttatcca aaacaacaag ttcgtgaaat gttaaaagct
    41941 ttggatgcag atattgcaca attattgatc gtttcaccag actactttga agttatggca
    42001 gcagatgaac aagtgccaga taacgctatg gtatttgatg atgttgctat tacagtggaa
    42061 aaagcagacg gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt aggagtagat
    42121 gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt ctatcctgaa
    42181 gctgtagctg aaggattcga agaaaagtaa aagggaaaag ggggtgtgac atgttctttg
    42241 tcacaccccc ttttgttatc aatcgtttaa ttttccacgt tcacgatacc agttatctgg
    42301 ttgccagatc cattcgaatc cgtctttttc aagtagttcg aaagctgctt taggtcccat
    42361 tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt ttcgaatgac
    42421 atccacgata tgccaagatt gagcgacttc ttcccaatga gagaagttcg ttccatcacc
    42481 attcaacgca tctaatagta atttttcgta agcttctggg ctattttcaa ccatttctgc
    42541 actatttcta aattccaatt tgattggttc cgtttcaaat ccttgcccta cttctttccc
    42601 attcaaggat aaagaaaatc cttcagttgg ctgaatatag atcgttagaa cattaggtgg
    42661 aagtgtcgta tcgtcacatg gttcatcgac agatgttttg aatacattaa caggaacttg
    42721 tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg tacgtacata
    42781 gaaaggaaca cctgaccaac ggaagttatc gatagtgaat ttgcctgcga cgaatgtttc
    42841 agtctctgat tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt gtacaccatc
    42901 caattcacct tgagcatatt gcccgcgaac gaagttttct aacacttctt cttcgttata
    42961 aatgcggata gctttcaatg ctttgatctt ttctgtacgg atttcttttt cggaaaatgc
    43021 tactggcggt tccattgcaa gtaatgctac aacctgtaaa atatgatttt gcaccatgtc
    43081 tttaagtgcg ccactatgat cgtagtagcc cccgcgatct tctacaccga ggttttcgcc
    43141 aaaagtaatt tggatattgt cgatatagcg attgttccat tgagcctcaa aaatattatt
    43201 ggcaaaacgg atagctgaga tgttttggat catttctttt cctaaataat gatcaatcct
    43261 gaaaatatct tgttctggga atacttctct gatttgctgg ttcaattcaa aagcagactg
    43321 gtaatcagaa ccgaacggtt tttctatgat caaacgattg aagccatttt ttgtcataat
    43381 attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca tagccaaata
    43441 atagacacgg ttaccgttta attgatagtg tccatctaat cggtcagaca attcttttaa
    43501 tgtctgataa tgcgttgaat catttacatc atgggattga tagtaaaaat ggcttgcaaa
    43561 ggtagttgct tcttcatctg aaggattcaa tgcacggatt gtttctttga caacttcacg
    43621 gtagtgctca tcgctccagg gacggcgagc ggttccgatt acagcaaaat gctcgcctaa
    43681 atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg ctaggtcacc
    43741 tgtaccgccg aatatcgtaa acaatacatt tttttcatta gacatgtgga tacactcctt
    43801 tatattttat cgacaaaaat cgtgctggcc gccttaaagc tgacggggat tttctttcta
    43861 tcattttgga tagtgatcgg tccttcataa gctgcgatgt caacgatctc atattcttcg
    43921 ttgatattca ggtcaatagt gactaagtaa tccaacaatt ctttttcatc aagcactcgt
    43981 gcgatacgca cttttgtacc gacaggatag gactccaatg tttgtcggcg ttcttcatgg
    44041 acaacttcct cttttttagg aatcgatcct ccatgaggac aatgttcagg atgattcaaa
    44101 tagttttcta aatgatctgc aagatgttcc gaagtcacat gttccaatac ttcagcatca
    44161 tcatggactt cattccagct atagtctaaa tgttcaacta aaaaaacttc ccataagcga
    44221 tgtttgcgaa tcagactgct agctttttgc agtccgcttg cagtcaattg tacaccttga
    44281 taaggggaat gttctacaag ctcttctttc acaagtttag aaatcatttc actgacagat
    44341 gcagctgaga catctagact agatacgatt tgtttattgt tgactttcgt tgtatctcca
    44401 cctagttcta aaatgatttt caaataatct tctcgatttg gtgtcatatt cgatcatcct
    44461 ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt gactattttt
    44521 ctaaataaaa aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa gatgatagag
    44581 aagactcatt ctttcgttta taaaggatat atatatacat tgggagggat ctatttttca
    44641 gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc gggataagaa
    44701 aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc tatttctacc
    44761 acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt aatctgtaat
    44821 tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga gagtccttct
    44881 gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac ggtaatgagt
    44941 gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga gaaaataatc
    45001 gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa aatttctcta
    45061 agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata catagtagga
    45121 agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta ttgaccgccc
    45181 gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg cgcatgtcaa
    45241 aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta ttgtatttgc
    45301 tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga agggtgactg
    45361 gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt tagacggcac
    45421 gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag taacttatct
    45481 tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat ttgttgaagt
    45541 caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc ccacactcag
    45601 aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg ccgcttatgt
    45661 cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag aaatgcaacc
    45721 agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc tcgcctataa
    45781 ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg atgtagatat
    45841 cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact taggtattat
    45901 cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa aaaaatatga
    45961 attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt ctcattatga
    46021 aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc atatggacac
    46081 catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag caattttggc
    46141 aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa tggatgaaat
    46201 catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa atatccatga
    46261 atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa tcggtgcaaa
    46321 tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag aagaaccgaa
    46381 tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt accttgtata
    46441 tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa gaaagatttc
    46501 tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg atgtatcgat
    46561 ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg actcaggaac
    46621 gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc gcgcgcataa
    46681 agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg aagcagagga
    46741 ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt atgaagagtg
    46801 gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa atggcggcat
    46861 caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca agtatttccc
    46921 gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct tattcaacga
    46981 gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc tacaggttca
    47041 ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg tgaagcaagt
    47101 gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga tgttgcacgt
    47161 gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc caacgtcaaa
    47221 cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc aggaagaata
    47281 ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat ggtatttgat
    47341 gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat tcttcatcaa
    47401 gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc ccttgaaagc
    47461 attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga ggacgattct
    47521 cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga tgactacttg
    47581 agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca actgactgaa
    47641 ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag tctggcctct
    47701 gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa aatggtcaga
    47761 gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct tccttatgta
    47821 gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg aagaactggt
    47881 cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct aagagattta
    47941 aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata cggaggtaag
    48001 ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa aaagtatgcg
    48061 aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat aagaaaaaaa
    48121 gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata agcgcctttt
    48181 tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt tatgtgaaag
    48241 aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg acagcaagtg
    48301 atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta agtgaactat
    48361 acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct ttgccaacgg
    48421 aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact tttgaaacac
    48481 tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct gcgattcttt
    48541 atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg aagtcgatgt
    48601 ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca gatgcgccat
    48661 ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt ggacacatct
    48721 ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt ctaatagcca
    48781 aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg gaagggcagg
    48841 aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa agtgcacaag
    48901 tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa agtttcttat
    48961 ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct tcacgttcac
    49021 tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat cgtttgaatc
    49081 aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat gcggaaggaa
    49141 atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt ttacattacc
    49201 gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt caagtacaaa
    49261 aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg gacagcaaaa
    49321 gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa gatccagaac
    49381 gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc gaaaccggtg
    49441 tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat ttccagacat
    49501 atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat gaaatctggc
    49561 ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt tataatgaaa
    49621 aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg agagaaggtc
    49681 atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg gaattccagg
    49741 tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt ccttttgatg
    49801 aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag cctgttaaag
    49861 aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca agcatgttgg
    49921 agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg ccactggtta
    49981 tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat caagagcatt
    50041 tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc agtgatttga
    50101 tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc atgattgtga
    50161 tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat caaaatatcc
    50221 agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct gaaactttac
    50281 agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct gccaaattat
    50341 atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag atgcagtttt
    50401 tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat gagatcaaag
    50461 caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca gcctatgacg
    50521 tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag atcccgacag
    50581 ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt gatgaactga
    50641 tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt atcgtggaac
    50701 ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga ttgatcaaac
    50761 aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat cgtttggaaa
    50821 tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta aaaattccag
    50881 aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa aaatgaacac
    50941 actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa gagctaaaaa
    51001 gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt tatataaaag
    51061 tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt agtttgttca
    51121 gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga agaaaaaggg
    51181 atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt agaatctaag
    51241 aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca atttgaacaa
    51301 aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta tggcatgatg
    51361 aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa gtatgcagtt
    51421 gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg ttaatcatgt
    51481 ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa atcggtgatt
    51541 ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa gcgcatcatg
    51601 cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg acattattga
    51661 ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta gcaaaagaaa
    51721 tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt tgaacggaag
    51781 aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct ttatataaaa
    51841 aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga aatacatcct
    51901 gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg atgcttttat
    51961 cggcatactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg caattattcg
    52021 tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag acaaaggaat
    52081 ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg gtacactagc
    52141 aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag cttatggagt
    52201 aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta tttcctttgg
    52261 tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag atgcaatcaa
    52321 tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat tatggggttg
    52381 gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca tgggagctat
    52441 ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa tgcctgattc
    52501 agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga tcgctttata
    52561 cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac tgatcattga
    52621 tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt tgggagcagt
    52681 attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg gatcaaatgt
    52741 attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata ttgatatttt
    52801 ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa tcgaagatgg
    52861 aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg gcggatcagg
    52921 cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag attatctaac
    52981 agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag tgatgtttgg
    53041 tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc cagtagtagc
    53101 aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat cgcagcaagt
    53161 cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg attggcgggc
    53221 accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac catttgtcat
    53281 ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac gtgaggagcg
    53341 ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa atgattacaa
    53401 ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac aatgaagaag
    53461 gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa acaagtgcag
    53521 aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag ccttggggaa
    53581 cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat aggatggttg
    53641 agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg ttgtatagtt
    53701 tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt tttctaacag
    53761 gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag gagaaaaata
    53821 actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg ataaagttat
    53881 ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta gaaaacagct
    53941 gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca taaaaatcga
    54001 agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga atgaattcgt
    54061 cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt acacagaaaa
    54121 aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa caatcttgat
    54181 gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa cgtattaaat
    54241 caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag gaatttttca
    54301 taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag gggcagcaaa
    54361 atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt tgatttagcc
    54421 acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg tacacttgcg
    54481 gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag tcaagtcatg
    54541 gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg cattggctat
    54601 gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat ggaaaagatc
    54661 cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt ctcctatccc
    54721 ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca actaaacagt
    54781 cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga ttttgaagat
    54841 gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg agctggtctg
    54901 aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta ccagtttgct
    54961 ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct tgcaacgtct
    55021 tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag ccttgttcgc
    55081 ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg ctacggcggt
    55141 aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac accttatatg
    55201 atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa aaagagacaa
    55261 gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga aggaatcgtg
    55321 gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac aaaactccca
    55381 atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg taaagtggaa
    55441 gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac gttaagctca
    55501 atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac gggtgatgac
    55561 aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa tgttaaagta
    55621 aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg ttttcttttt
    55681 tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat gaatctaagc
    55741 gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc cctacattat
    55801 ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt gcctaactta
    55861 taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct acaattgatt
    55921 tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag cgataggtcg
    55981 ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg gtacaggggt
    56041 ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata taaactatcg
    56101 aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat atgaagataa
    56161 gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt
    56221 actccttgat aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa agtaaattgc
    56281 ttttcaatcc ctaattcttt taattttttg cgcaaatatt tgctgacggc gtttgatgaa
    56341 actaatccat tttttaagtt gaaaaaaaca aaattatgag ggtttttgat ttcaaaattc
    56401 tcgaataatt ctttttgatt gattttaaac ttttttaaga gatcaatcgt atgtttatca
    56461 atggaaattt tcctatttga agtttcgttt tttgtttttc cccattcatt taatttataa
    56521 ttccatgttt tattaatagt gatgatttga tcttcgaaat cgatatcttc ccatgttaat
    56581 cctagaagtt cagcaaatcg cattccagtg gcaccagcaa ctaacacgag cataggagaa
    56641 gagtactgcg cagaaaggtt ttcttctacg agtttcatta aagatttaaa ttgatcataa
    56701 tctaaatatt tatcttcttt tggcttcaat gaatcttttc ctttgattac tgcttttcta
    56761 gttggatcaa acggaattaa tccttcttct acggcatctt tcaacgacgc tctaatatgg
    56821 ttattaaact taataacggt agattttaca tggtctttag catacttatt taaaaagcgt
    56881 tgatatccca ctctatctaa atcagaaatc aataccgctg gcatatattt ctttatgttc
    56941 attaacgtat cttcgtattt tctataagtg ataggagata ctgttccttc tttataaagt
    57001 tgcatccaat cctcaaagta atctgataat aatagatttt tgcgatcccc tttaaggcct
    57061 ttgttcaagt tatattctaa ttcgttggcg gcatcttttg cttctccttt tgtcttaaat
    57121 cctgattttc ttatcttcgt gtattttccg tcatcttttt tgtatgaaat ttcgtattgc
    57181 caactatttc cacgtttgac taatcttgcc ataattgaat cagctctctt tctctgatac
    57241 aataggaact ataaagaagc ccattgtttg ggttttatta ttttttgcac atcctttttg
    57301 cttgccggcg caaggatgtg ctatttgtcg ttttaatggt ataattggat gtgtattaga
    57361 tcatccaatc agaggtgttt attatgatta aagtgatttt taaaagaata actgaacttc
    57421 cagagtttag attatttgtt gattattggt atgtctggtt ttttattgtt gcattgctga
    57481 taggtctaga tgtcatatta gaaagaaaac ttaagtaaga gttgagttaa taaattgaat
    57541 tcctaaatga gatattttgt agcccatatt ttgtttgccg acatcaccta atactatgga
    57601 gctaatcgtt ttttctaaca gaccaaaatc agataatcta tttaagctac catctattag
    57661 ctcttgtgaa tagccagaca tgctgatatt tccagtgaaa ttttcgtttc cacgactcat
    57721 taagcatgct aacaattgaa tctcaatcga tgttaattga ttcagtacaa gaagataata
    57781 ctcctcgtta tcccatgaag cgttgttaat tcgaagtaaa caattcttat aaagattttt
    57841 atacatatct attttatttt gtgcctttga tttttctacc tcgtcattta tcccttcgaa
    57901 tattccaatg agttggtcct tgttctctaa gttgaaaaca tcttctggaa tttgatcgcg
    57961 aatgttttct aatgattcgt ttaaattttt gtagaatttc tcgatgcgtt tgaaacgctt
    58021 ctcgttttta gacccaaaat aaagtgactg cacaacgcca ccaatataag gaattgctcc
    58081 aataccgcct tcaactatta attcaagata ttcttttttg gttagttttt cattgctatt
    58141 atccatagtt acctcctatt ttgtcatttc ctttacaagc attgtgactc ctgatggcac
    58201 tcttttgtat aggttttatt attgtgcaca atctaactct tagtggggcg tggttcattt
    58261 attttttact tactttacta ttaatcatat cccattgagc ataggatttt tctatgttcg
    58321 atcctcttga actaatttct cctgtaaaag ttactttatc gccctttgta aaggtatcag
    58381 cattgtttaa gtccttcgca aaaattacat atgaaacttt aggtgttgca gaaatatcat
    58441 tccaactttt atcatcgtac tctttgtctg ctataatggc tattctagta gtcattgatt
    58501 cgataacagt tccttcaaaa gtatatgttt taccactaat atatttagta aaagctgtat
    58561 tttgctcagt agctggagta atagaataat aactatctaa gaaagcaccc cagtcatgat
    58621 tatttgtctc taaaaattct tcaaattgag ttaggctttt attgctgctg ttagatgtag
    58681 tagtttgagt atcagatgat gagctttcct gtttgttatt gtcatttgcc tcgtttgatg
    58741 aggaaagcga gaaacctacg gtagtaaaaa taatcccgat aataaggaga atcataggtg
    58801 ttttcttatt tgtttttttg ataaatctta caattaacat gattaaacct ataataaata
    58861 aaacaatacc agtaaaacca aatataccgt ccattaatag atcctctttc tcttttgata
    58921 ttgaatatta taaaccaagc aattcttttt tcttaaaatc gaattcttct tgattaataa
    58981 tcccttcatc taacaactct ttatactttt taatttcatc tgcacttgaa atttgtgttt
    59041 ctttggaatt attatctaat atagattgtt gcttctctat atattttttt aaattttcca
    59101 tttcattcca atattttttt gtaaacataa cggtgttttc atctttcgtg gccgctaata
    59161 tacctccgcg actttcgttt ccgcctagca aggtaaattg tatataacca ttactcatac
    59221 ctggtttttt taattgtacg gctgatatat ttttaaaggg gatagttttt tctcctttaa
    59281 taccttgatt aactaaattt ataaatcctt ttctaataat tgaaatagaa tcatcagtta
    59341 ctttaatcaa ggtttttcct ggagatttta ttaaaatctc tttttccatt ttttcttcct
    59401 catttctgtg atatgatttt ttttgtagaa gctcatatat gaggaaagag cccgtgttgc
    59461 agcacgggct tttttaatgt tttgctgaaa tcggtttttt taataattct tgatgtattc
    59521 atacatattg ccttgcgtaa gaatattttt tcttaaaata gcattggcag acagcataac
    59581 aagtgtatct gagcttattc tgaatagaat aatactccat aaatttttcg agattaaact
    59641 gagattcatc agtcagttcg ttctcaatat agatatttaa taaaattaga atagctattt
    59701 tatctgcttc tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac aaaactgaaa
    59761 attcaaaatg agaggcgctg aaatgtgcaa gttcatgaga taaatgaaag gcttctgcag
    59821 tttcaccgta tagattttca ttcaaaaaaa tgattctggg ttttggatag tagaaaccag
    59881 gctctttcat ctccatataa actactttca aattgtattc gctcaacatt tctttcaatt
    59941 tcaaatacat acaaaccatc actccaacta ttcattttcc tctaaagctt tagcaattgc
    60001 aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa aaacagtatc
    60061 gtcttctgat aaatccacat gtttaggggt ttctctttct tctctaccta gaaggtagtc
    60121 tacagagaca tcgaaatagt cagcaatccg acttaattca tctgaattag gagtattatt
    60181 tttccacttt gctagataac cgttagaata tccaaaatta atttctaact gccgtataga
    60241 tatctttttc ctcttcgcca agtcttttat tatttcatag gtattcatcg ataaaacaac
    60301 ctttctaaat gtttacaaaa aaagtttaga aaaatacgca gaaataattt gactaactct
    60361 gagtaatgag ctatactata tcttgtaaac agatttaaca actaaaaaga caacaaaaaa
    60421 cactattgat atgtaaatgc agaccgccaa gaaagcttta aaatcaatgt ttttatgtct
    60481 tatttaatta tgctcttagt atagagtatt actcagatac tgtcaattga atttagaaaa
    60541 aagttgttaa atttgtttac gaatataaaa gaaaggaatg agaaatatga acacaccgca
    60601 aatttttaat ttcgaacaaa acgaagttcg gacaatttta gtaaatgatg aaccatattt
    60661 tgtaggcaaa gacgttgcga gtgttttggg ttattcaaac actaaagatg ctttgtcgcg
    60721 gcatgtagat ttggaagata agatggggtc gcgaatcacg acctcaggtc aatcaagaga
    60781 gatgacaatc atcaacgaat ctggtttgta cagtttaatc ttaaaatcaa aacttccctc
    60841 tgccaaaaaa tttaaacgtt gggtaacaag tgaagtgttg ccagcaatta gaaaacatgg
    60901 aggttatcta actccagaaa aagtagaaga agctttgctt aatccagata caatcattca
    60961 attagcaact caactaaaag aagaaagaac tggaagatta atcgcagaac aaaagattgc
    61021 agagtacgaa cctaaaatct cctatttaga tagcatatta tcttctacag attcagtaac
    61081 aattagtcag attgcagcag attatgggat gtctccacaa cagatgaata aattacttca
    61141 taaactaggt attcagaaaa aagtcggtaa ccaatggtta ttgtgcaaaa aacacatgaa
    61201 ccaaggatac acaaaatctc atacaactga gatcccgaaa gccgatggtg gcactaaaat
    61261 tgtaatgaat accaaatgga cacagaaagg gcgtctattt atctacgaat tactaaaaaa
    61321 agaaggatat taccctcaaa tggatttaga ggaaattggt tagaaaggag ttttagtatg
    61381 actgacattg cagaaatcac tcaacgagat agagaaaaaa tcaaagaata tgtcgaaagt
    61441 tcaaagttct taacttacac catgcttgct gaaagatttg gaattagcaa aagctactta
    61501 tctttaattt taaacggtaa aaagacttct gcagaagcaa acagaattat agattcgatt
    61561 atcactatgt acgaattgta aagggaggaa cagctaatgc aatatctaga agcaaaaatc
    61621 ccaattccag aaggctatgt aattatctcc caagtggatt atgaggagtt aaaaaaagct
    61681 gatgatactg gtagatggat gacgttgcca gaagtgctag aacggattaa cagaaaatat
    61741 gattggttta cttctagagt tttaaagaac ccaagatata gaaatattat cgatatcgag
    61801 aaaaacaaaa atggatttgt ctattatcca gttgaaggaa gagacacata tctattttta
    61861 agaagtaaaa cacttgaatt tttagaaaca aatttttcgg aaatcttaag gaggcaagcg
    61921 gatgggaaaa tttaatagag cgctagtgtt cagcgcaccg ctaatcatct acgctttagg
    61981 actttgggga agcaggcaag cgttgatagg aacgattgtt tacatggttt ggatttttat
    62041 ggggcttgat gaag
//
LOCUS       DANKMN010000005        61003 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000005 DANKMN010000000
VERSION     DANKMN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(151..2304)
                     /locus_tag="PW224_000326"
     CDS             complement(151..2304)
                     /locus_tag="PW224_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL2907043.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="PW224_000327"
     CDS             complement(2598..3446)
                     /locus_tag="PW224_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2907044.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="PW224_000328"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="PW224_000328"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL2907045.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="PW224_000329"
     CDS             complement(5947..6126)
                     /locus_tag="PW224_000329"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL2907046.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="PW224_000330"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="PW224_000330"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2907047.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="PW224_000331"
     CDS             8274..10553
                     /locus_tag="PW224_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2907048.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="PW224_000332"
     CDS             complement(10646..11293)
                     /locus_tag="PW224_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907049.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="PW224_000333"
     CDS             complement(11472..12191)
                     /locus_tag="PW224_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2907050.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="PW224_000334"
     CDS             complement(12322..13473)
                     /locus_tag="PW224_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL2907051.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="PW224_000335"
     CDS             complement(13690..14430)
                     /locus_tag="PW224_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2907052.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="PW224_000336"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="PW224_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL2907053.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="PW224_000337"
     CDS             complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="PW224_000337"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL2907054.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16041)
                     /locus_tag="PW224_000338"
     CDS             complement(15391..16041)
                     /locus_tag="PW224_000338"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL2907055.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="PW224_000339"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="PW224_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL2907056.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="PW224_000340"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="PW224_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL2907057.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="PW224_000341"
     CDS             complement(17484..18011)
                     /locus_tag="PW224_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL2907058.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="PW224_000342"
     CDS             complement(18191..18976)
                     /locus_tag="PW224_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL2907059.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="PW224_000343"
     CDS             complement(18969..19838)
                     /locus_tag="PW224_000343"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL2907060.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="PW224_000344"
     CDS             complement(19839..21218)
                     /locus_tag="PW224_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL2907061.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="PW224_000345"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="PW224_000345"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2907062.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="PW224_000346"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="PW224_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL2907063.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="PW224_000347"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="PW224_000347"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL2907064.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="PW224_000348"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="PW224_000348"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL2907065.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="PW224_000349"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="PW224_000349"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2907066.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="PW224_000350"
     CDS             complement(25176..25400)
                     /locus_tag="PW224_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2907067.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="PW224_000351"
     CDS             complement(25453..26415)
                     /locus_tag="PW224_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL2907068.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="PW224_000352"
     CDS             complement(26425..26865)
                     /locus_tag="PW224_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2907069.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="PW224_000353"
     CDS             27171..28136
                     /locus_tag="PW224_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2907070.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="PW224_000354"
     CDS             complement(28193..28423)
                     /locus_tag="PW224_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL2907071.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="PW224_000355"
     CDS             complement(28509..29396)
                     /locus_tag="PW224_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2907072.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="PW224_000356"
     CDS             complement(29485..30708)
                     /locus_tag="PW224_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2907073.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31771)
                     /locus_tag="PW224_000357"
     CDS             complement(30725..31771)
                     /locus_tag="PW224_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2907074.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31935..33662)
                     /gene="ptsP"
                     /locus_tag="PW224_000358"
     CDS             complement(31935..33662)
                     /gene="ptsP"
                     /locus_tag="PW224_000358"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL2907075.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33662..33928)
                     /locus_tag="PW224_000359"
     CDS             complement(33662..33928)
                     /locus_tag="PW224_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL2907076.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34407..36641
                     /locus_tag="PW224_000360"
     CDS             34407..36641
                     /locus_tag="PW224_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2907077.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36789..37109
                     /locus_tag="PW224_000361"
     CDS             36789..37109
                     /locus_tag="PW224_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2907078.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37314..37538)
                     /locus_tag="PW224_000362"
     CDS             complement(37314..37538)
                     /locus_tag="PW224_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDL2907079.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37736..39316)
                     /locus_tag="PW224_000363"
     CDS             complement(37736..39316)
                     /locus_tag="PW224_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL2907080.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39717..41093)
                     /locus_tag="PW224_000364"
     CDS             complement(39717..41093)
                     /locus_tag="PW224_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2907081.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41221..42339)
                     /locus_tag="PW224_000365"
     CDS             complement(41221..42339)
                     /locus_tag="PW224_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2907082.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42481..42915
                     /locus_tag="PW224_000366"
     CDS             42481..42915
                     /locus_tag="PW224_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2907083.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(42993..43526)
                     /locus_tag="PW224_000367"
     CDS             complement(42993..43526)
                     /locus_tag="PW224_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL2907084.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(43620..44798)
                     /gene="mutY"
                     /locus_tag="PW224_000368"
     CDS             complement(43620..44798)
                     /gene="mutY"
                     /locus_tag="PW224_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL2907085.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(44791..45588)
                     /gene="recX"
                     /locus_tag="PW224_000369"
     CDS             complement(44791..45588)
                     /gene="recX"
                     /locus_tag="PW224_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL2907086.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            45695..47077
                     /gene="rlmD"
                     /locus_tag="PW224_000370"
     CDS             45695..47077
                     /gene="rlmD"
                     /locus_tag="PW224_000370"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2907087.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(47251..47829)
                     /locus_tag="PW224_000371"
     CDS             complement(47251..47829)
                     /locus_tag="PW224_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL2907088.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            48151..48621
                     /locus_tag="PW224_000372"
     CDS             48151..48621
                     /locus_tag="PW224_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL2907089.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(48729..49955)
                     /locus_tag="PW224_000373"
     CDS             complement(48729..49955)
                     /locus_tag="PW224_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2907090.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(50709..50909)
                     /locus_tag="PW224_000374"
     CDS             complement(50709..50909)
                     /locus_tag="PW224_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2907091.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(51043..51651)
                     /locus_tag="PW224_000375"
     CDS             complement(51043..51651)
                     /locus_tag="PW224_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL2907092.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(51736..52206)
                     /locus_tag="PW224_000376"
     CDS             complement(51736..52206)
                     /locus_tag="PW224_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL2907093.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(52254..53168)
                     /locus_tag="PW224_000377"
     CDS             complement(52254..53168)
                     /locus_tag="PW224_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2907094.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            53314..54117
                     /locus_tag="PW224_000378"
     CDS             53314..54117
                     /locus_tag="PW224_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL2907095.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            54295..55242
                     /locus_tag="PW224_000379"
     CDS             54295..55242
                     /locus_tag="PW224_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL2907096.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(55310..55603)
                     /locus_tag="PW224_000380"
     CDS             complement(55310..55603)
                     /locus_tag="PW224_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL2907097.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(55631..56296)
                     /locus_tag="PW224_000381"
     CDS             complement(55631..56296)
                     /locus_tag="PW224_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL2907098.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(56436..57068)
                     /locus_tag="PW224_000382"
     CDS             complement(56436..57068)
                     /locus_tag="PW224_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2907099.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(57075..58142)
                     /locus_tag="PW224_000383"
     CDS             complement(57075..58142)
                     /locus_tag="PW224_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2907100.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(58139..58870)
                     /locus_tag="PW224_000384"
     CDS             complement(58139..58870)
                     /locus_tag="PW224_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL2907101.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(59039..59518)
                     /gene="greA"
                     /locus_tag="PW224_000385"
     CDS             complement(59039..59518)
                     /gene="greA"
                     /locus_tag="PW224_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL2907102.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(59654..60829)
                     /gene="mltG"
                     /locus_tag="PW224_000386"
     CDS             complement(59654..60829)
                     /gene="mltG"
                     /locus_tag="PW224_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL2907103.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT        17531 a        12875 c        10313 g        20284 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tctgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agccctattt ttgtctattc ttactgacaa
    31861 aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc
    31921 ttttgatacg ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcata
    31981 tcgcattctt tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc
    32041 attaaactac gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata
    32101 cctactagca atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat
    32161 tttccttcag cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta
    32221 tatggttggt acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga
    32281 atcaaatcgt tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag
    32341 actgctgctg ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg
    32401 ccttcactta tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct
    32461 ttgagtgtag ctaccatagg gaacatgata cgtaaattac catgaacaga tgcacgaagc
    32521 aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat
    32581 gcacgatatc ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta
    32641 tctccaccga tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca
    32701 gctttgtaag cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg
    32761 aattcagtac ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct
    32821 tttggtgttc cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct
    32881 gcatttttta atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca
    32941 aattcagctt tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca
    33001 gccaaaatat cgccttcttt gactttagca gtaatttctt ttgtcccaac gattgctgga
    33061 atttctaaag aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca
    33121 aacgctttta caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca
    33181 acaacgacca cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc
    33241 gctaaaatac gtttagcaac gtcacgaata tctgcagcac gctcttgcat ataagcatta
    33301 tcttccattg cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca
    33361 gcatttacgc tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa
    33421 agaaccatca aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct
    33481 ttttcacgaa tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct
    33541 tcttcagccg cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc
    33601 aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc
    33661 attattcaga taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt
    33721 cgtcagcacc ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa
    33781 cgcccatgat tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt
    33841 taaatttgct tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg
    33901 ctactacgtg aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt
    33961 ttatgtgagg gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat
    34021 ctgtaaagat taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa
    34081 agtattcaaa ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg
    34141 tttatttcct ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac
    34201 cctctttgtt ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag
    34261 agtcaaaggt caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa
    34321 aaaactaggg gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca
    34381 tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa
    34441 cgattcactt gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct
    34501 gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc
    34561 catttggatt tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc
    34621 aaggaaatta tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg
    34681 gtcagccacc tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag
    34741 aacaagcacg acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag
    34801 tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg
    34861 ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc
    34921 aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg
    34981 gtatacgagg acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag
    35041 aaaatgtgat cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg
    35101 atggaaatat ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa
    35161 tggttggtgc tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac
    35221 gtcgtatgca acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga
    35281 aaggtttgca aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg
    35341 aagcagccgc aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca
    35401 tcgatttgct agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta
    35461 aaacaatcga gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag
    35521 aagatttcga aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag
    35581 aacgtcagct cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg
    35641 ttgaacaacg gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga
    35701 aaaacttagc agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag
    35761 tagcgaaagc gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct
    35821 ccttcttatt tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct
    35881 acgaactatt cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa
    35941 aacatagtgt ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg
    36001 gtcaattaac agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag
    36061 aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga
    36121 cagatgcaca aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg
    36181 ccggaactgg taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc
    36241 gttctgtatt gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg
    36301 ggatcatcga attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc
    36361 tagacgatgt caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg
    36421 tcaaagaaaa attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac
    36481 gcacgatcca agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta
    36541 tccatgagtt gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag
    36601 aacgtttggc taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga
    36661 gacggtattt ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt
    36721 cattttataa aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg
    36781 tgagcgccat gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc
    36841 ttgaaaacac tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct
    36901 acactgaagc ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc
    36961 cgaaagaaat cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc
    37021 acgaggatga aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac
    37081 atgaattagg cgctacatta gccgtgtaaa caaattttga acataataat taaagagcgt
    37141 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca
    37201 gaagcaactc cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga
    37261 attccagctt atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt
    37321 tttgattttt ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct
    37381 ttatttttaa ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg
    37441 tttttgtccc agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc
    37501 tgtttttttc tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta
    37561 atcatatgta cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat
    37621 atagtaaaaa gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg
    37681 tttttgtttg gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca
    37741 tcaaactttt taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt
    37801 tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac
    37861 tcatcttctc atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag
    37921 acatgattac ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta
    37981 gctgaccaac agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg
    38041 ctccttcttg aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt
    38101 ttgcagtcac tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt
    38161 ttagttttcc ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga
    38221 tgatatcgcc tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga
    38281 cattgcttaa tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga
    38341 atgttccaga acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg
    38401 cttggatctt gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat
    38461 cttgtgtttt gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct
    38521 gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa
    38581 ttttttcttg attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt
    38641 gttcatataa ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca
    38701 attctactaa tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt
    38761 agatatcata aagaccttct agccctttac ccataccgat tggccaattc atcggataag
    38821 attcgatatc taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac
    38881 gatctagttt gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata
    38941 atttctttgt ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca
    39001 cggccatcaa agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga
    39061 tatttactcg tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac
    39121 gttgtttctc gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta
    39181 cggtcccggc ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt
    39241 tcccagcatc cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta
    39301 attttggatt attcataacc taactccatt tcttccattc aattatttat attttctcta
    39361 ggcacagatt gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc
    39421 gtgtagcagt ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc
    39481 ccacccaagc caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca
    39541 ggatagcttt ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca
    39601 gcgaatgaaa aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag
    39661 aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat
    39721 cttcttggct cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc
    39781 gcttctgttt cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc
    39841 atttcttcag aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc
    39901 ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc
    39961 agatcactgt caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat
    40021 tcgtgttcat cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct
    40081 ccgacgattt cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca
    40141 tctaataaaa tagccatctg attctgcgtc tttttcattt catataacag atcatcaata
    40201 aagaccgttt caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca
    40261 aagccgaatt tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta
    40321 tctttgtctt cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca
    40381 ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact
    40441 tctcgggcaa ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta
    40501 ttcaatacgc cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct
    40561 tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc
    40621 cagacgaagg gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg
    40681 acttcttctg atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat
    40741 acataggtca aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca
    40801 cctaataccg gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg
    40861 ttgaccaaag tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt
    40921 tttaaaagct tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta
    40981 ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg
    41041 atcaataata aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca
    41101 ttctctcctt ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca
    41161 cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac
    41221 ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt
    41281 gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc
    41341 aagcaatccg gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat
    41401 tttcagtgat ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat
    41461 tagcacaacc acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa
    41521 tgcaggtgct agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat
    41581 gaccccaaat aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt
    41641 accaacgaat aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag
    41701 ttgccccaat aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt
    41761 tggcactagt ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat
    41821 tactacggtt gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact
    41881 aaggaattgc tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat
    41941 atctacttcc ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt
    42001 gatgaaatca ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac
    42061 actaagtaaa atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca
    42121 tattcttttg atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag
    42181 aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa
    42241 aataaagttt atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga
    42301 ccaaaacatt agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct
    42361 tactagtata tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt
    42421 gccatacttt ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc
    42481 atgaaagttg tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc
    42541 aggcatcaag tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat
    42601 gaagcccatt gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta
    42661 cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa
    42721 tacagacacc aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat
    42781 caaggctata atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa
    42841 ctaggatata cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
    42901 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
    42961 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
    43021 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
    43081 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt
    43141 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
    43201 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
    43261 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
    43321 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
    43381 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
    43441 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
    43501 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
    43561 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
    43621 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
    43681 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
    43741 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
    43801 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
    43861 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
    43921 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
    43981 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
    44041 gctccagaat gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt
    44101 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
    44161 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
    44221 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
    44281 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
    44341 actcgcatca cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc
    44401 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
    44461 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
    44521 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
    44581 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
    44641 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
    44701 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
    44761 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
    44821 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
    44881 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
    44941 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg
    45001 gatcacttct tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc
    45061 tttttggctt tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
    45121 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
    45181 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
    45241 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
    45301 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
    45361 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
    45421 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
    45481 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
    45541 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
    45601 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
    45661 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
    45721 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
    45781 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
    45841 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
    45901 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
    45961 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
    46021 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
    46081 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
    46141 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
    46201 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
    46261 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat
    46321 cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
    46381 aaaaaacgca atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca
    46441 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
    46501 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
    46561 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
    46621 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
    46681 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
    46741 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
    46801 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
    46861 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
    46921 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
    46981 taaaaagttc aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg
    47041 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
    47101 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
    47161 ctgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
    47221 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
    47281 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
    47341 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
    47401 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
    47461 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
    47521 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
    47581 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
    47641 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
    47701 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
    47761 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
    47821 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
    47881 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
    47941 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat
    48001 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
    48061 ttatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
    48121 aaactaatat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
    48181 gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
    48241 ggaaaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
    48301 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
    48361 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
    48421 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
    48481 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg
    48541 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
    48601 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
    48661 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
    48721 atttttttct agtacccact tgttgttcca tactcattat tctggtcata accataacca
    48781 ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac
    48841 tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg
    48901 ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc
    48961 gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta
    49021 ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga
    49081 agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc
    49141 aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg
    49201 tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa
    49261 ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga
    49321 cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa
    49381 cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag
    49441 tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc
    49501 aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg
    49561 gccatttcga caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct
    49621 acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga
    49681 ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg
    49741 gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca
    49801 gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct
    49861 tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct
    49921 aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct
    49981 aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact
    50041 ttaaaaaaca tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta
    50101 tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat
    50161 ctttttttat gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg
    50221 cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga
    50281 ttatcattcg atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt
    50341 ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc
    50401 attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt
    50461 aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt
    50521 tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc
    50581 ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat
    50641 ctaaaaactt cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca
    50701 acaaattatt ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc
    50761 gtgaattcta cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag
    50821 aaatgaacaa atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg
    50881 ttgaaccatt taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa
    50941 gtaaccttgc aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta
    51001 ttttccaaat tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa
    51061 acgtttgttt acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg
    51121 gacattttta tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt
    51181 ctttccttcc atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt
    51241 ttccattaca agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc
    51301 ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt
    51361 aggttcgcca ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc
    51421 gccaccattg ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa
    51481 taattcttct attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt
    51541 cgtcacataa gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata
    51601 aggttccaat gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt
    51661 cctcctcaat gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt
    51721 cttcaattgt tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc
    51781 agtttcttta aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg
    51841 agtgatgatt ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc
    51901 ccccatgtcc aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag
    51961 tgcatgtgta aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc
    52021 aaacaataca gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata
    52081 ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag
    52141 atagactagt atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg
    52201 cttcatgagc caacctacct tattaaaaat tcttatttat catactctat atttcaataa
    52261 ctttcatctt tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg
    52321 atcgtcacat gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc
    52381 ttttcaatct cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc
    52441 ttttcgctat actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca
    52501 atttcttgtt ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca
    52561 gcttttttga tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata
    52621 actggatcga tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt
    52681 accgaagaca gtgtatcact taacaaatgc aaataactag attttatatt caaactttca
    52741 tctgcttctt gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct
    52801 actgttagca tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca
    52861 acggttaaaa acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt
    52921 tctaaacgct ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt
    52981 gccccgtaag aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca
    53041 agagacaaac tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc
    53101 aaaaccacta ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct
    53161 tcctccattt tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc
    53221 accaatcatt cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt
    53281 gcaagtgatg acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt
    53341 tcctttatac gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt
    53401 gtctatcttt tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa
    53461 gtcttaaaca acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca
    53521 atccaaaacg ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc
    53581 attatcttca catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg
    53641 atggggaatt ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat
    53701 acaggcacga cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa
    53761 tctttaaaaa acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca
    53821 ctttggataa aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg
    53881 atcactttgc tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa
    53941 gctacttttc ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt
    54001 gcaacaatct tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca
    54061 ggaatcttta tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa
    54121 ataaagacaa ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt
    54181 gtcacagccc cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc
    54241 aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca
    54301 gaaagaatca tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact
    54361 atagcgcctg gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag
    54421 atcagtttgg gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa
    54481 tgggtccaat ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa
    54541 catattttac tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta
    54601 ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg
    54661 aaatggttag gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt
    54721 ggaactggcc atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc
    54781 tatggaatca atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat
    54841 caactaaagg aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag
    54901 gaaacaactg ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc
    54961 atcaatgttg gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac
    55021 cagcaacttt cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat
    55081 cctctttgga aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat
    55141 tttcaaaaga aattaatggc tattttttta accaatctga aaagctactt tgccacccat
    55201 gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt
    55261 tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt
    55321 taaagtgata ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt
    55381 taaagaacca ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct
    55441 ttacaggatg ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg
    55501 ttttgccgta gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg
    55561 agtcaaacca cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc
    55621 tcttttcttt ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa
    55681 taaccagtag attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg
    55741 tttgatctac tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt
    55801 cagctaatgt tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt
    55861 tatctgacaa ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta
    55921 ttcccatgtc tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg
    55981 catgatactc attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca
    56041 gagtaactaa gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat
    56101 ttcttatgcc taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag
    56161 tggctatatt catatattca ttgacccgat cttcattact atcaatagcc agtacttctt
    56221 gaccagcttc gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg
    56281 caaaattttg tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc
    56341 tttacaaaaa caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt
    56401 ttctcagctt ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat
    56461 gcatagatag cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca
    56521 tgtgttttta ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt
    56581 ccttcagaaa tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca
    56641 ggctcatttc gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact
    56701 cgctcgccac gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc
    56761 aacaaatacc ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa
    56821 ctggttacga taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg
    56881 ataccatcca tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc
    56941 gccttctcat agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga
    57001 atagataaat aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact
    57061 tttatcatcc ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct
    57121 tgacctttaa aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata
    57181 ttgttcaatc cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca
    57241 ataatacgta acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct
    57301 ttagcatgtc ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca
    57361 atggaactag atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt
    57421 tgcaattcct tcaataactg ttcgatacct tgctttaaac ttttctcttc taaattgact
    57481 ggacgtagat gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct
    57541 accattttca actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc
    57601 aatgccgaca tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca
    57661 cgggctaatc gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat
    57721 tggggacggc tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc
    57781 ttagtgattt ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga
    57841 agaagcaaca atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt
    57901 ttcccgtatt ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct
    57961 aaggaaatgg ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat
    58021 tctccccacc aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata
    58081 ataaggaaag ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc
    58141 atattcgtat cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag
    58201 ggttttcatc ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct
    58261 cttcttcaaa caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca
    58321 aaggtactaa gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt
    58381 ttggcaaaag cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga
    58441 tatcgtccca ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt
    58501 tccgctcatt attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc
    58561 gccaaaaagc atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa
    58621 ataaaacggc aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa
    58681 tgaccaaact tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca
    58741 tagctagata tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta
    58801 cgatttgcca aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag
    58861 aactattcat cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt
    58921 aactgttggc tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag
    58981 agaaaaagat ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact
    59041 acgcttgttc tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta
    59101 cttcatcatt tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt
    59161 tgccagaaaa cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat
    59221 ctggtagttc gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt
    59281 cgttatcgat gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa
    59341 caaatgcttg ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac
    59401 cgcgggcaat tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta
    59461 attctttttc taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt
    59521 gtaacgtctc cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc
    59581 aaaaaagatt accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct
    59641 agagcctgtt tttctaagaa ttatttacat atttttccac taattccata tgttcttcat
    59701 aagttttagc gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat
    59761 tttctgctgg ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag
    59821 gtccatagcc ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg
    59881 tcactaattc tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca
    59941 tatttgcttt gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta
    60001 cgccttcttt ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct
    60061 gcttgatagc tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca
    60121 ctagatcctt aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt
    60181 ccaaacggta ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt
    60241 ggtgcaactc gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt
    60301 taccagttac cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg
    60361 caattttccc atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc
    60421 caatctcgtc aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta
    60481 aatttttaaa ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct
    60541 ccaaaatctc accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt
    60601 ggtcatctga tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc
    60661 caagaaatcc gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa
    60721 tcttatcttc ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct
    60781 tactggcttc ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc
    60841 cttactatca agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat
    60901 tttatttaaa tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata
    60961 aatgttatca agatacaagc tattttagct ttattctttc tgt
//
LOCUS       DANKMN010000006        55502 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000006 DANKMN010000000
VERSION     DANKMN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            558..3197
                     /locus_tag="PW224_000387"
     CDS             558..3197
                     /locus_tag="PW224_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2907104.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="PW224_000388"
     CDS             complement(3365..4063)
                     /locus_tag="PW224_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2907105.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            4281..5426
                     /gene="tgt"
                     /locus_tag="PW224_000389"
     CDS             4281..5426
                     /gene="tgt"
                     /locus_tag="PW224_000389"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL2907106.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            5523..5903
                     /gene="yajC"
                     /locus_tag="PW224_000390"
     CDS             5523..5903
                     /gene="yajC"
                     /locus_tag="PW224_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL2907107.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            6208..8805
                     /gene="adhE"
                     /locus_tag="PW224_000391"
                     /gene_synonym="adhC"
     CDS             6208..8805
                     /gene="adhE"
                     /locus_tag="PW224_000391"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL2907108.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            9034..10140
                     /locus_tag="PW224_000392"
     CDS             9034..10140
                     /locus_tag="PW224_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2907109.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            10157..11185
                     /locus_tag="PW224_000393"
     CDS             10157..11185
                     /locus_tag="PW224_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL2907110.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            11191..11670
                     /locus_tag="PW224_000394"
     CDS             11191..11670
                     /locus_tag="PW224_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL2907111.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            11686..12981
                     /locus_tag="PW224_000395"
     CDS             11686..12981
                     /locus_tag="PW224_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL2907112.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            12992..13816
                     /locus_tag="PW224_000396"
     CDS             12992..13816
                     /locus_tag="PW224_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2907113.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            13981..14880
                     /locus_tag="PW224_000397"
     CDS             13981..14880
                     /locus_tag="PW224_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2907114.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(15006..15875)
                     /locus_tag="PW224_000398"
     CDS             complement(15006..15875)
                     /locus_tag="PW224_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL2907115.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            16028..17488
                     /gene="rhaB"
                     /locus_tag="PW224_000399"
     CDS             16028..17488
                     /gene="rhaB"
                     /locus_tag="PW224_000399"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL2907116.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            17485..18741
                     /gene="rhaA"
                     /locus_tag="PW224_000400"
     CDS             17485..18741
                     /gene="rhaA"
                     /locus_tag="PW224_000400"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL2907117.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            18755..19600
                     /gene="rhaD"
                     /locus_tag="PW224_000401"
     CDS             18755..19600
                     /gene="rhaD"
                     /locus_tag="PW224_000401"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL2907118.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            19602..19913
                     /gene="rhaM"
                     /locus_tag="PW224_000402"
     CDS             19602..19913
                     /gene="rhaM"
                     /locus_tag="PW224_000402"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL2907119.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            19995..21146
                     /gene="fucO"
                     /locus_tag="PW224_000403"
     CDS             19995..21146
                     /gene="fucO"
                     /locus_tag="PW224_000403"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL2907120.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            21273..22031
                     /locus_tag="PW224_000404"
     CDS             21273..22031
                     /locus_tag="PW224_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL2907121.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            22131..22775
                     /locus_tag="PW224_000405"
     CDS             22131..22775
                     /locus_tag="PW224_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL2907122.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            22782..23789
                     /locus_tag="PW224_000406"
     CDS             22782..23789
                     /locus_tag="PW224_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2907123.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            23805..24647
                     /gene="kduI"
                     /locus_tag="PW224_000407"
     CDS             23805..24647
                     /gene="kduI"
                     /locus_tag="PW224_000407"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL2907124.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            24667..25470
                     /locus_tag="PW224_000408"
     CDS             24667..25470
                     /locus_tag="PW224_000408"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL2907125.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25719..26783)
                     /locus_tag="PW224_000409"
     CDS             complement(25719..26783)
                     /locus_tag="PW224_000409"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL2907126.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26780..27865)
                     /locus_tag="PW224_000410"
     CDS             complement(26780..27865)
                     /locus_tag="PW224_000410"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL2907127.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="PW224_000411"
     CDS             complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="PW224_000411"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL2907128.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="PW224_000412"
     CDS             complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="PW224_000412"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL2907129.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            30108..30767
                     /locus_tag="PW224_000413"
     CDS             30108..30767
                     /locus_tag="PW224_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2907130.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            30852..31757
                     /locus_tag="PW224_000414"
     CDS             30852..31757
                     /locus_tag="PW224_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2907131.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(31759..32391)
                     /locus_tag="PW224_000415"
     CDS             complement(31759..32391)
                     /locus_tag="PW224_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2907132.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    32542..32667
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            32711..33235
                     /gene="infC"
                     /locus_tag="PW224_000416"
     CDS             32711..33235
                     /gene="infC"
                     /locus_tag="PW224_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL2907133.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33307..33507
                     /gene="rpmI"
                     /locus_tag="PW224_000417"
     CDS             33307..33507
                     /gene="rpmI"
                     /locus_tag="PW224_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL2907134.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            33560..33919
                     /gene="rplT"
                     /locus_tag="PW224_000418"
     CDS             33560..33919
                     /gene="rplT"
                     /locus_tag="PW224_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL2907135.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            34171..35508
                     /locus_tag="PW224_000419"
     CDS             34171..35508
                     /locus_tag="PW224_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2907136.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            35556..37685
                     /locus_tag="PW224_000420"
     CDS             35556..37685
                     /locus_tag="PW224_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL2907137.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            37660..38349
                     /locus_tag="PW224_000421"
     CDS             37660..38349
                     /locus_tag="PW224_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907138.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(38757..39370)
                     /locus_tag="PW224_000422"
                     /pseudo
     CDS             complement(38757..39370)
                     /locus_tag="PW224_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(39506..39700)
                     /locus_tag="PW224_000423"
     CDS             complement(39506..39700)
                     /locus_tag="PW224_000423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907139.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(39750..40190)
                     /locus_tag="PW224_000424"
     CDS             complement(39750..40190)
                     /locus_tag="PW224_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL2907140.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(40194..41039)
                     /locus_tag="PW224_000425"
     CDS             complement(40194..41039)
                     /locus_tag="PW224_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL2907141.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            41188..42606
                     /gene="pepV"
                     /locus_tag="PW224_000426"
     CDS             41188..42606
                     /gene="pepV"
                     /locus_tag="PW224_000426"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL2907142.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(42663..43610)
                     /locus_tag="PW224_000427"
     CDS             complement(42663..43610)
                     /locus_tag="PW224_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2907143.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(43833..44183)
                     /locus_tag="PW224_000428"
     CDS             complement(43833..44183)
                     /locus_tag="PW224_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2907144.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            44383..45462
                     /gene="pepA"
                     /locus_tag="PW224_000429"
     CDS             44383..45462
                     /gene="pepA"
                     /locus_tag="PW224_000429"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL2907145.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            45606..45926
                     /locus_tag="PW224_000430"
     CDS             45606..45926
                     /locus_tag="PW224_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2907146.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            45948..46412
                     /locus_tag="PW224_000431"
     CDS             45948..46412
                     /locus_tag="PW224_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2907147.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            46617..47222
                     /locus_tag="PW224_000432"
     CDS             46617..47222
                     /locus_tag="PW224_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL2907148.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(47261..48550)
                     /locus_tag="PW224_000433"
     CDS             complement(47261..48550)
                     /locus_tag="PW224_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL2907149.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            48978..49457
                     /gene="rlmH"
                     /locus_tag="PW224_000434"
     CDS             48978..49457
                     /gene="rlmH"
                     /locus_tag="PW224_000434"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL2907150.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            49542..50051
                     /locus_tag="PW224_000435"
     CDS             49542..50051
                     /locus_tag="PW224_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDL2907151.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            50151..50801
                     /locus_tag="PW224_000436"
     CDS             50151..50801
                     /locus_tag="PW224_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907152.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            51252..52190
                     /locus_tag="PW224_000437"
     CDS             51252..52190
                     /locus_tag="PW224_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2907153.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            52203..53255
                     /locus_tag="PW224_000438"
     CDS             52203..53255
                     /locus_tag="PW224_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2907154.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            53521..54156
                     /locus_tag="PW224_000439"
     CDS             53521..54156
                     /locus_tag="PW224_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2907155.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            54347..55138
                     /gene="msrA"
                     /locus_tag="PW224_000440"
     CDS             54347..55138
                     /gene="msrA"
                     /locus_tag="PW224_000440"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2907156.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT        17619 a         9922 c        11894 g        16067 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaataac accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctttt
     4141 ttttgttatt cataaaaaaa ggataaaaaa aagaatatta aataaagtag aaggaagccg
     4201 agggaacttg caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta
     4261 tatagaaagt agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag
     4321 aaaaacatac tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac
     4381 caatgttcat gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga
     4441 aagaaatggg tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg
     4501 aagacttagt agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt
     4561 taacagattc aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag
     4621 aaggcgtgca tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag
     4681 caatcaacat ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac
     4741 cattcgatga aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag
     4801 aacgaggatt gaaagctcat gctaatccag accgtcaagg attatttgga atcatccaag
     4861 gagcagggtt tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc
     4921 ctggttactc aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat
     4981 tagaattcac tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg
     5041 ctgctgattc attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc
     5101 ctactcgaat cgctcgaaat ggcacatgta tgacttctca aggccgtcta gtagtgaaaa
     5161 atgcgaaata tgcccatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta
     5221 aaaactatac acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc
     5281 gtttgacttc ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag
     5341 caatcatgga tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca
     5401 ataaagaaaa cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag
     5461 tgttatcagc atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa
     5521 aaatgggaac attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta
     5581 tgtcacgttc gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac
     5641 caggtgattc agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg
     5701 acaaacggac agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa
     5761 tcaaaacggt caaaccaggg actgtcgtaa caaatgatgc aacgactact gttgaaacaa
     5821 cagaagaacc agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga
     5881 cagaagagaa ggaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa
     5941 gatgtcctac ctcatttttt attcaaaaaa gaaagagata agagcatggg atatattgct
     6001 caatattgaa taatcgggtg tgtcatataa ataataataa ataactttat atatttttct
     6061 atacaaacac ctgtaaataa agcaatttta tatttaaaaa taactgtgaa aaaaatcaca
     6121 aaaaagtgtt tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa
     6181 taaccatgac ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag
     6241 acaattgacg tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa
     6301 gaaatggaaa gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca
     6361 gctttggatc aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt
     6421 tacgaagata aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa
     6481 catgataaaa cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct
     6541 gaaccagtcg gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt
     6601 ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt
     6661 gcccaaaaat cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga
     6721 gctcctgaaa attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta
     6781 atgaaccatc caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct
     6841 gcttactcta caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt
     6901 gaaaaaacag caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat
     6961 aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca
     7021 gtaaaagcag aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa
     7081 ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat
     7141 tctgcgatgg aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg
     7201 attgctgaat tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca
     7261 gttcttgcca tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg
     7321 ttgaatcttg gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat
     7381 gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa
     7441 ggcggcatcg gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct
     7501 tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact
     7561 gtagcgaaac ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa
     7621 aagaactctc tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt
     7681 gaccctggta tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt
     7741 aaaaatgatg tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc
     7801 gtatacgcag gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga
     7861 ggcggttctg caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca
     7921 tctttctttg gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac
     7981 aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta
     8041 acgccatttg cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat
     8101 gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct
     8161 gtcacagcag ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc
     8221 atggcttcag attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat
     8281 ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct
     8341 acaatggctg gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat
     8401 aaaatcggcg gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat
     8461 attatccgct ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat
     8521 ttccgtgcag atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca
     8581 acagaggaat tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc
     8641 atcaacatga gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac
     8701 cgtatggctg aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg
     8761 atctctgaat tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta
     8821 gaaaaaagat aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt
     8881 tttttaattt ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag
     8941 acagtttttg ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata
     9001 taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta
     9061 cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa
     9121 gacgataatg gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg
     9181 acagtgtgga attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg
     9241 acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa
     9301 ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat
     9361 gaagacacga aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg
     9421 aatgacatgc ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat
     9481 aagaatcagt tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg
     9541 aaactgttga atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc
     9601 aaatacttga tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat
     9661 cataactatg ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca
     9721 gagatcattg aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa
     9781 acacttgaag cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat
     9841 acgttgttgg atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac
     9901 ggtattttga aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat
     9961 cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca
    10021 atcgggacag gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg
    10081 ccttatggac agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa
    10141 aagaaaggaa acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct
    10201 aggtctgcta ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc
    10261 agatgtaaat gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat
    10321 tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat
    10381 ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc
    10441 aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta
    10501 ttcgattttt tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga
    10561 taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt
    10621 gacctattat gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc
    10681 ggaagatttg gagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt
    10741 agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca
    10801 atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg
    10861 tggtgtggct aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat
    10921 gaatgatgcc atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc
    10981 gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc
    11041 tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga
    11101 ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca
    11161 aaagatccaa gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag
    11221 atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc
    11281 agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc
    11341 tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg
    11401 gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt
    11461 ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg
    11521 acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta
    11581 cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga tacattaagc
    11641 aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc
    11701 ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata
    11761 agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct
    11821 tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg
    11881 tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat
    11941 tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg
    12001 aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga
    12061 gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat
    12121 atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca
    12181 cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct
    12241 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
    12301 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
    12361 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
    12421 gcttcagcga tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta
    12481 aaactaaaag atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg
    12541 ttcttattgg ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag
    12601 gcaattagtt cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc
    12661 aatcttgtgt tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt
    12721 acaccaatat ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg
    12781 ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat
    12841 gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt
    12901 tatggggtta tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg
    12961 ctacccaata tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga
    13021 tctttccgaa gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca
    13081 tacactgcag ctcgccttag gtctttcgtt tcctgaaaca ccttctggcg caaatgagat
    13141 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
    13201 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
    13261 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
    13321 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
    13381 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
    13441 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
    13501 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
    13561 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
    13621 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
    13681 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
    13741 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
    13801 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
    13861 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
    13921 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
    13981 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
    14041 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
    14101 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
    14161 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
    14221 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
    14281 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
    14341 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
    14401 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
    14461 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
    14521 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
    14581 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
    14641 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
    14701 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
    14761 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
    14821 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
    14881 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
    14941 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
    15001 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
    15061 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
    15121 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
    15181 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
    15241 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
    15301 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
    15361 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
    15421 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
    15481 tcccctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
    15541 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
    15601 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
    15661 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
    15721 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
    15781 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
    15841 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
    15901 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
    15961 ttgttaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
    16021 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
    16081 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
    16141 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
    16201 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
    16261 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
    16321 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
    16381 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
    16441 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
    16501 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
    16561 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
    16621 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
    16681 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
    16741 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
    16801 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
    16861 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
    16921 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
    16981 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
    17041 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
    17101 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
    17161 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
    17221 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
    17281 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
    17341 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
    17401 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
    17461 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
    17521 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
    17581 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
    17641 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
    17701 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
    17761 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
    17821 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
    17881 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
    17941 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
    18001 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
    18061 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
    18121 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
    18181 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
    18241 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
    18301 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
    18361 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
    18421 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
    18481 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
    18541 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
    18601 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
    18661 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
    18721 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
    18781 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
    18841 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
    18901 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
    18961 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
    19021 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
    19081 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
    19141 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
    19201 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
    19261 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
    19321 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
    19381 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
    19441 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
    19501 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
    19561 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
    19621 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
    19681 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
    19741 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
    19801 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
    19861 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
    19921 caactgggca accggttgct tggggttctt tcgtttttat tgtgaaaaaa aatacaaatt
    19981 aggaggaata gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag
    20041 gagcgattga gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca
    20101 ttactgacaa aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata
    20161 aagcagcaat cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg
    20221 taaaagatgg agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg
    20281 gaggttcgcc aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt
    20341 cagacgtcat tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag
    20401 ctataccaac gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg
    20461 aagtaaatca ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca
    20521 tcgacagcga tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg
    20581 ctatgacgca tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc
    20641 tgcatttaaa agcaatcgaa atcatcggcc attctttgga agtttctgta gatggcgatc
    20701 aaaatgggcg agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg
    20761 tcggtttagg actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc
    20821 atggcgtagc gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg
    20881 gagaaaagta ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt
    20941 tagaagatgt aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa
    21001 tcccagaaac catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag
    21061 atgctttgcg agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca
    21121 ttgctttata tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt
    21181 aaagagaaat ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata
    21241 atttttctta taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg
    21301 tattgatcaa agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc
    21361 agacgatcgc aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt
    21421 tgttgatgat cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc
    21481 ttattcgcta cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt
    21541 ttttagaaca gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca
    21601 atgagatctt gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa
    21661 aaatcggaat cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat
    21721 tcagtgaaga gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat
    21781 atacgatcac caatgcatta cggttgaaaa aggaaatcca aacggtaaaa gaaacccaca
    21841 tcgcatttga cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga
    21901 aaaacgaaca aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg
    21961 agacaaaaga acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa
    22021 ttcaaaaata atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata
    22081 gatttccaag ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa
    22141 ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca
    22201 aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt
    22261 tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag
    22321 accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg
    22381 ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga
    22441 tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa
    22501 cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa
    22561 gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg
    22621 tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg
    22681 ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac
    22741 agtatgcagc aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac
    22801 tcggagaaat catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac
    22861 gattcgctgc ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg
    22921 gacatgaagc gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa
    22981 agcacttgca atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag
    23041 gtacttatta tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg
    23101 ccggatcaag ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag
    23161 gaatagatat atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa
    23221 tgacgaaaca ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca
    23281 actatcgtgg aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac
    23341 ctttagtaga tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga
    23401 aagcgccaga agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt
    23461 tcccgaatat tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg
    23521 aattgaaggg gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca
    23581 ccccaatcgt ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga
    23641 ttttatccaa tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga
    23701 agcatacgat ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag
    23761 catgcggctc tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa
    23821 cacgttatac acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag
    23881 aatttttagt agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg
    23941 atcgaatgat ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca
    24001 aagaactagg tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg
    24061 gaccaggatt catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact
    24121 atatcggaaa agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta
    24181 aattctatat tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg
    24241 accaagtaaa accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc
    24301 aatacatcca tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag
    24361 aaccaggaag cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt
    24421 atgtctactt tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag
    24481 atgaaacaaa acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga
    24541 tccattcagg tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata
    24601 tcacctatac agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag
    24661 aaaatgatgg aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc
    24721 ggtgctgtct atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa
    24781 atcgtattta ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa
    24841 gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg
    24901 atggttcaac agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg
    24961 atcatcaaac ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac
    25021 gtggatttaa atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa
    25081 ggcggcggta aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc
    25141 agtgcttatg ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa
    25201 tacggtcaat acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa
    25261 actgctccat tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc
    25321 gttggacgta cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc
    25381 ttcttggctt ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc
    25441 atacttgcct atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag
    25501 gttgtgaaat cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc
    25561 gaaaaaagag gctgggacag aagcatttaa ctccaagaaa taagaaggaa ttcacgaaaa
    25621 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ttacttccgc
    25681 cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
    25741 tctgtgcgtc ttccatactt cactaagtcg atccctcttg cttcgcacca atccttcact
    25801 ggtccggaaa agatcagaga ttgttggtgt agggccgcgg tattcgttgc gccgaccata
    25861 gtataaagca gacgaagctc ttcttgccat tgcttcatca gtataatagt ttcttctacg
    25921 ccgtgattca tcagatgcgt cagtacggtt cctgaagtac ccacggcatt tgcgccgaga
    25981 caaagggctt taaatatgtc ataggcattg cggatacctc cggaggctag gatttccata
    26041 gaagtgtctg cttcatttgc ttcaagcaat gaagagactg tcgattgccc ccagtcagct
    26101 aaataactca gttcccgctt ggagcgacgg gcgttttcga tctgggtgaa gcttgttccg
    26161 cttcgaccgc tgatatcaac tgtatgaact cctaaagcag ctagatcatt gagcgtttct
    26221 cttgtcatgc caaaaccgac ttcttttacg atgaggggga cgtcaatggc tgtttgagtt
    26281 tcttgaataa gtactttcca attggtaaaa tcacggtctc cctcaggcat aaccaactct
    26341 tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa acaatcggat agcttctttg
    26401 gctctttcga cagaagtccc tgcaccgatg ttggcaatga ttttcccgtg aggatattct
    26461 tgtcgcataa tcgtataagt atcggctaaa gaagggactt tcaaggcagc gctgactgaa
    26521 ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg caagatcttg gttgattttt
    26581 tttgtttttt cgcttccgcc agtcatcgca ttgatataaa atgggctgct tagtgtaaac
    26641 ccaactgcct gtgtacttag atcgacatca gaaactgcta tttgcggcaa agggttatgg
    26701 atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat gaaaggcttt agctagtgaa
    26761 acatgttcgt cttttcgatt catcggtttt cctttctttg atcgctatag acatgtaacg
    26821 gcagtggagt gatttctgct ttttcccatg cactcattaa aggaagaatg ccagatttct
    26881 ggtcaacaat tacgattccg caatcgcccc cacctgcacc agaagatttt gcggctcctt
    26941 catactgttc agctaaatta cacaatttgt tcaaagcagg cgtttcgatg acgaccccag
    27001 tgattgcaga taaatcatgc agtaattgtc ggttttttcg aatcatctgt tggatcaacg
    27061 ttacattatt ttctttaaac cctttgatca tttcgttgac acattctgta ctatttttta
    27121 agaaaagctg ataagccacc attttatctt ctctcgaacg gtgaacttga tcgaccaaat
    27181 cagaagtaga ggcagggcta cccgtccaac caatcaataa acgtaaatct tcaggagcaa
    27241 tcaatggctc aatggataga cctggccaat ccagggcaag taactcactg atagaatgct
    27301 gagtttcttg ttcttggagc caaggatggt cgaaggttga gaaagcgatc cagccaccat
    27361 aacagctagc agcgatgtcg ccgcaggaac cattatcttg aactgctaaa ttggctagtg
    27421 ctgcaatctt gaaaatctcc aactgagata aattcaacgc gtaaaaaaca ttcaaggctt
    27481 tgaccgttgc aacggtcacg gcaccgctag aacccaatcc atatttccgg ccatttgaac
    27541 tgtctaactc acttgttact ttcagatcat aaaaggataa aagaatgttt ttttcttgtg
    27601 catacttttc agtcaagcga atagcagcaa ggatataatg aaaaggattt tcccgaatat
    27661 ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg catcccacta tattgtgcag
    27721 attggatact tccgactttt cgtgcagatt ctacagtgac tgtcacgaat tgatcgactg
    27781 cagcgataac tgctggatgg cctgtttcaa caactgcata ttctccggca atatagagtt
    27841 tgcctggtgc agatacttca atcataaaaa tctcccttta ttcaataatc gcaattcctg
    27901 gaccagcatg tgcagtaatt acttgttctt tgctaaataa tttggtaaat gtttcttgta
    27961 cctcagataa atggctgttt tccactaaaa ctttgacatt agggccagca tccatcgtaa
    28021 agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc atccatcgct tttaagctgt
    28081 caggagacca gtaagtgaat ggaggctggg cagctaaagt cgttccatgc atttttagtc
    28141 cgtttctttc catcgtttct ccgagaagtt ggaaatcttt tgttttgatt gcttgtttca
    28201 attgatatag atccccttca acggaatcta accagccttg ataaaaactg gatgtttcga
    28261 ctgtccgacg catcccattt ctgctggaca cttctttttt ctggtcgttg atcaaaacga
    28321 aaaccattgc taaatcgtct tcgaaagaat cggaagggac tggcttagcg taagaactta
    28381 agtcgtcatg gcctttttcc cattcgacga aaccaccgaa aatacttcgg caagctgaac
    28441 cagacccgcg tcgagcaaat cttgaaagag agagatcatc taatccaagc ttaagagcag
    28501 tattgcaagc gcctgctaaa gcagctagcc cgctggcaga ggaggctaat ccagctgcgg
    28561 taggaacgaa attctgactg attactgaag cttttagtga gaggccggct tctttacgaa
    28621 aaaggtcaag aaactgactg actttttttg ttgctttttc gtcttgcaag gtgccatcta
    28681 gataaaattc atctaccata tagctatcag aaaagatgac ttctgtctct gtataaaatg
    28741 catccaatgt taacgaaagg ctgttgttca ttggaaggat gagttcttca tttttcttac
    28801 cccaatattt gattagagca atgttggtat atgcgcgtgc tttgccttta aacataggta
    28861 tgtactccta atccttgtat ccaagtttca gcagcacccg catcttcaag tgcttgactg
    28921 atttcttggg cttttgtttt tgtttgtgct aatgcaatca tacagccacc gcgtccaccg
    28981 ccagttaatt tggcacccaa tgccccggta tcttttgctg tttggatcaa tagattaaga
    29041 aaatcattgc tgattgtcag cttttccaga agtgattgtg attcatccat tgtctgtgcc
    29101 aacgtttctg gtgaattttc aatgattgct tgttttgctt gcttcgtcaa gtatcctaat
    29161 ttttggatca tttgtccagt ttcatgaggc tgggtttcaa agaggtgagc gacatctttg
    29221 acggcttctc ttgtttgtcc tttgattcct gtatcagcga caatcaaaaa agcatcgatg
    29281 ttcaaagaaa agtagtcgaa aggatgccct tttgtaaaat aaatcggctg caagctgcta
    29341 gtagcggctg catcgattcc actaggatta ccgtgggcga ttttttccga aagctggaca
    29401 ttttctaata gtatttcacg agacaaagga aatgctaagt aatcataaaa agcacgagtg
    29461 actgctgtgg cgactgcagc gcttgatccc attccacgtt cagcaggaat cgtgctttca
    29521 atggtcagtt gcaagttttt ttggatgtca tgctgttttt ttaagtgaat gaataatttt
    29581 ttgatatttt ttaatgcatg aggagcttcg gctaaatttc ctgaatagta ggaagaaacc
    29641 agttgatcgt ccattgcatc cagctcttca aggaatgcgg tgacttttgt tgcatagaaa
    29701 ggaaaggcaa tcgctggttc tccataaaca accgcgtgct cgcccatcaa tatgatcttt
    29761 ccgcttgatt ctccttggcc atagtttgcc ataatctaac tccttctcac gtaaaagtga
    29821 tttttaagca aaaaataaag cgcccaaaac gagttgcgct gcttcacgaa tttttttgtc
    29881 cactaagggt ctcaaaaaac attcctcctt tattttacta aaaccatctc agaagtaaaa
    29941 ctaagaatac gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt
    30001 cttctttgac tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa
    30061 ggttggagtt aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg
    30121 ttttagcagg tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact
    30181 ttgatagtca ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg
    30241 caaaccctga acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa
    30301 gcatcaaaca agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt
    30361 cactgctttt ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt
    30421 atgatgaact gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc
    30481 cggatttatt agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac
    30541 gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat
    30601 tgaacagacg ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg
    30661 atggagaccg ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga
    30721 tcgaagaaaa attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc
    30781 aaataaaggg taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag
    30841 gaggatagag tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg
    30901 tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg
    30961 agatgctgac ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg
    31021 atgaagcgtt actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg
    31081 aaactagtcc tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga
    31141 ataccgagta cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg
    31201 tatacagttt attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg
    31261 aggaagtgaa tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca
    31321 agaatctgct gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa
    31381 aacaaaatgt agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag
    31441 aaaaggaaaa agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga
    31501 cgaacttagc ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata
    31561 acaacttgaa tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa
    31621 ctattgtgac aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg
    31681 gatggggaat cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt
    31741 ggcggcggat tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag
    31801 tgcttgctct tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt
    31861 gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt
    31921 caactgattg tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc
    31981 taattgacaa tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga
    32041 agcaatacaa tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt
    32101 taatttacat tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga
    32161 aaaatcacat ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg
    32221 catcgtaagt ttttggatat gactctctct taataccata tttttggcat cgagatagct
    32281 ttggtcaccc ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc
    32341 tccctttgta agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca
    32401 cctcgttttt ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct
    32461 aatcattttc agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt
    32521 tgctatacta tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat
    32581 aatgtcactg ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg
    32641 taagagaact cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat
    32701 ttggaggtga atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga
    32761 gttacgattg atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt
    32821 gaatattgca gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc
    32881 agttgcgcga atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga
    32941 agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga
    33001 cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa
    33061 agtgaaagct tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa
    33121 agtcttgaat cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa
    33181 aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga
    33241 atgactaact ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat
    33301 atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt
    33361 aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa
    33421 aaacaacgcc gtcaattgcg taaagcaagc atggtttcta gtggcgactt caaacgtatt
    33481 cgccaacaac tagcaaaaat gaaataagaa gtacaaataa cagaatctga agatttattt
    33541 ttattaggag gaattataca tggcacgtgt taaaggtgga acagtaactc gtaaacgtcg
    33601 taaaagagtg ctaaaattag caaaaggtta ctacggttca aaacacactt tatttaaaac
    33661 agcaaaagaa caagtaatga attcatacaa ctacgcatat cgcgatcgtc gtcaaaagaa
    33721 acgcgacttc cgtaaattgt ggattgctcg tatcaacgca gcagctcgta tgaatggctt
    33781 gagctactca aaattaatgc acggtttgaa agttgctgaa atcgacatca accgtaaaat
    33841 gttagctgac ttggctgtta acgatgcagc agcatttact gcattggctg accaagcaaa
    33901 agaagctttg gctaaataag ctaaacaatt ataaagagga aagaagataa cgaaaagtta
    33961 tgttctttcc tcttttttta tttacaatcc tccatcaaat gaaacaggac aataacgtat
    34021 aagtctaaaa tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct
    34081 ttaatgaaag cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta
    34141 tcagaaaaac aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta
    34201 gttatctcat tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc
    34261 tttgccatca tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca
    34321 aacccggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg
    34381 ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt
    34441 gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg
    34501 gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg
    34561 atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca
    34621 ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc
    34681 tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg
    34741 gctgaaagct atacttataa tgactatttc caaattggta tgattgggat gattgtaagc
    34801 ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg
    34861 gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta
    34921 ttaccagttt taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca
    34981 ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt
    35041 cagtttgcga aattatttac agatggcgca gtagatgttg caccaatggt tggtttcttg
    35101 atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt
    35161 tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca
    35221 ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct
    35281 gtcgtattag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc
    35341 acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt
    35401 tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc
    35461 ggagcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt
    35521 cgataaaaac gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa
    35581 ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca
    35641 tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg
    35701 ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat gttgtgtttg
    35761 ttgcacattc taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg
    35821 gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat
    35881 tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg
    35941 tcaaacactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag
    36001 cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg
    36061 tttatgaagc agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat
    36121 tatatggatt gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa
    36181 tgctagatac ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt
    36241 tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg
    36301 ttttaaccat gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag
    36361 cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa
    36421 atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat
    36481 tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa
    36541 tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg
    36601 agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg
    36661 ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg
    36721 cagccaatgt ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg
    36781 cacgaaaagc catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag
    36841 aacagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca
    36901 agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat
    36961 tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag
    37021 tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc
    37081 cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg
    37141 aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa
    37201 aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga
    37261 tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag
    37321 tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa
    37381 atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg
    37441 cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag
    37501 atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag
    37561 caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg
    37621 aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa
    37681 cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc
    37741 taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt
    37801 atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct
    37861 tttgccgatg taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca
    37921 gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg
    37981 atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca
    38041 gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga
    38101 atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca
    38161 aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc
    38221 gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc
    38281 caagggatct tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat
    38341 tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacgat
    38401 gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt
    38461 tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttggaaaacg
    38521 gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa gttaaaaaca taagaagcta
    38581 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
    38641 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
    38701 ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat
    38761 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
    38821 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
    38881 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
    38941 tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc
    39001 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    39061 aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttt tccaactttt
    39121 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    39181 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    39241 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    39301 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    39361 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    39421 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    39481 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac
    39541 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    39601 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    39661 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    39721 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
    39781 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    39841 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    39901 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
    39961 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    40021 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    40081 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    40141 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    40201 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
    40261 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    40321 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    40381 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
    40441 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    40501 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    40561 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    40621 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
    40681 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    40741 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    40801 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    40861 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
    40921 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    40981 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    41041 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    41101 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    41161 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    41221 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    41281 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    41341 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    41401 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    41461 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    41521 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    41581 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    41641 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    41701 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    41761 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    41821 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    41881 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    41941 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    42001 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    42061 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    42121 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    42181 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    42241 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    42301 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    42361 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    42421 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    42481 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    42541 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    42601 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
    42661 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
    42721 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
    42781 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
    42841 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
    42901 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
    42961 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
    43021 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
    43081 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
    43141 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
    43201 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
    43261 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
    43321 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
    43381 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
    43441 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
    43501 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
    43561 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
    43621 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
    43681 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
    43741 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
    43801 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
    43861 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
    43921 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    43981 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    44041 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    44101 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    44161 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    44221 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    44281 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    44341 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    44401 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    44461 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    44521 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    44581 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    44641 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    44701 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    44761 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    44821 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    44881 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    44941 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    45001 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    45061 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    45121 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    45181 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    45241 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    45301 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    45361 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    45421 ttggataaat caacagtgaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    45481 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    45541 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    45601 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    45661 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    45721 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    45781 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    45841 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    45901 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    45961 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    46021 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    46081 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    46141 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    46201 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    46261 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    46321 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    46381 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    46441 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    46501 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    46561 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    46621 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    46681 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    46741 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    46801 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    46861 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    46921 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    46981 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    47041 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    47101 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    47161 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    47221 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    47281 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    47341 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    47401 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    47461 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    47521 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    47581 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    47641 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    47701 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    47761 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    47821 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    47881 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    47941 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    48001 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    48061 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    48121 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    48181 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    48241 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    48301 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    48361 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    48421 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    48481 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    48541 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    48601 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    48661 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    48721 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    48781 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    48841 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    48901 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    48961 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    49021 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    49081 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    49141 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    49201 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    49261 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    49321 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    49381 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    49441 cgccttatca taagtgatgc ggtttttatc attgatccta aaaatcttag caagacaaaa
    49501 agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac aatgataggt
    49561 tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa gcttttgagc
    49621 taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa gtacaggata
    49681 agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac caagtttttg
    49741 acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat aagccgatag
    49801 tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg ctaaaaaaat
    49861 taaaaatcgc cttgtctatg accactgatg atattttaga cgtttttgct gaggcagaaa
    49921 tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa cgaaatttta
    49981 agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt tataatcgga
    50041 gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat gagagctagt
    50101 ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt atgaatgatg
    50161 aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga tgtaggctca
    50221 attttgagct tcggggaaag atgtctttaa tacaacaaaa aaagtattta gcgaataaat
    50281 cagagtttac tctgggacat gttgaaaaat taatctcgga ttggattagt agtagaagcg
    50341 aatttacaaa aataaaacag ccgattaaat ttgacatgaa aaaacttttg ttaaataaga
    50401 gtgagatagg taatagagat caatatatta gagctaaagg gcaagaaata attgattcat
    50461 taggtgaaat gaggtcatac aattatttgt atgtaacaca tagagctgat ggaatggtca
    50521 ttactgtggg gaaatcttct tctaatgata tttttttaga tggagatctt ttttatcaac
    50581 tcaatacaaa tcacttgtct ggcactgaaa atataatttt aaggacggaa tatggcaatg
    50641 aaatatttgc aaaatatgat gagattttaa aaaattattt ggactgggct tggataattc
    50701 cggttgagtc aggagatgct aagaaactgg agagattatt gggcgatgaa ctaattaata
    50761 aaaaagtacc aattttaaat tattattccc atagacaata aaaggctatt tttcatgata
    50821 atactagtta taactgtaaa aatggaagaa aatctgggaa agaagttttt aaataagtgt
    50881 attgttcgat tttaataaat aataagcaat accatccgtc tgtgttgcgc aacatcgtac
    50941 tacaatgaat attttttcac aaaaagaaaa tcatccatcg aatgaatgga aggttttagt
    51001 atacacgagg ttaatgcccc ctcttttata atcagaggag tgaggttttt tctatgaatg
    51061 tttctatttt cttattgcta gtctagtttc tcttacgatg aaattgggat tctattccag
    51121 tttttattga tttgctgata aaataacttg aatggtggat ataaacatga taaataagag
    51181 aagaataaca aaacaaggtt gtgaaatgat tctcaaatga atataataga aagaaaaagg
    51241 aggaagaatc aatgaaaata gcaattgcag gcgctggcgc aatgggaagt cgctttgggt
    51301 taatgcttca ccaaagtggg aacgaagtat tattaatcga tggctgggca gaacatgtcc
    51361 aacaaatcaa agagcatgga ttacaagcaa attttaatgg taaagaggta gaagcaaaac
    51421 taccaatcgt tcttcaatcc gaagtagaaa aagaagatca agttgatctg attattctat
    51481 ttaccaaagc gatgcagctg gaaaaaatgc tgcaggatat ccaatcatta atcaaaaaag
    51541 atacagaggt cttatgtcta ttaaatggta tcgggcatga agatattatt gagaaatttg
    51601 taccaatgga aaatatctat attggaaata ccatgtggac ggcaggtctt gaaggtcctg
    51661 gtcaggtcaa attatttgga agtggttcag tagaattaca aaatttaggt gatggaaaag
    51721 aagcagctgc aaaaaaatta gcagacaaat tgtctgaatc aggattaaat gctcattttt
    51781 ctgacaatat tcactattct atttatcgca aagcatgtgt gaacggaaca atgaacggac
    51841 tatgcacaat cttagacgtt aatatggctg agctaggaaa aacatcgact gctcataaaa
    51901 tggtggcgac gattgtcaat gagtttgcca aagtagcagc agtagagaag attgaactag
    51961 atgtcccaga agtcattgca cattgtgaat cttgttttga cccggaaaca attgggttac
    52021 attatccttc aatgtatcaa gacttgatta agaaccatcg attaacagaa atcgattata
    52081 tcaatggcgc aatttctaga aaagggaaga aatatggtgt tgcgacgcct tattgtgatt
    52141 tcttgactga gcttgtccat gcaaaagaag atagtttgaa cgtaaaataa aggagagatt
    52201 gaatggaaac ttcacaaaaa atatcaccga aaatttttct gaataaagtc ctcgctggta
    52261 cagcaacagg aatcattatt ggattgattc ctaatgcagt attaggtgcc attttaaaat
    52321 attttagtgc gtatcccttt gcggtaacga tcgcacagtt agcagttatt tttcaactag
    52381 caacaccttt gatcattggt ggtttgattg ccttacaatt tggatttgat ccgatgagaa
    52441 tgatggttgt agccggagct tcttttgttg gttctggtgt agtcaaattc acacctgatt
    52501 tgggtgaaac gggtgtttac gttggtgctg gtacaggcga tattattaat acgatgctga
    52561 cagcatcgat tgctgtcggg ctgattttac tgattggaaa tagttttggg tcagtgtcga
    52621 ttgtgttaac accgattgtt gtaggtgtag gtgcaggttt acttggtttt tatttattcc
    52681 cttttgtaac agcaattact tctgcaattg gatcattgat taataacttc actaccttac
    52741 aaccgctgtt aatgtctatc ttgattggtt gttcctttgc ctttttgatt atctctccta
    52801 tttcaacagt agcaatcgga atggcaatcc aactagatgg tgtttctgct ggtgcggctg
    52861 cgatgggcgt agctgcaacc acttttgttt tagtagtgaa ctcttggaag gttaataagt
    52921 caggtgtgac aattgcgatt gctttaggcg ctatgaagat gatgatgcct aatctattta
    52981 gaaaacctgt gattcttatt ccttgtttag ttagtgcagt gattacagct attcctgtcg
    53041 ctctattttc tatttcaggt acgcctgctt ctgctggttt cggcgtggtc ggacttgttg
    53101 gtccgttggc ttctttagat gcagggctaa atggctttct tgttgttctt tgttggttag
    53161 tcatccctgt ttttgcggca ctaattactc aatttttatg cgagaaagta tttaaactgt
    53221 atgatcgaga agaagtcttc aaatttttag gctagtagat tttactaaga aattttcaat
    53281 caatggatgc gtaagtgaga tgtcaacaac atctgactta cgtttttttg cctttttgag
    53341 taggagattg atgctgacaa ctgaactctt cagtgccatt cgatttttta aaatgtagaa
    53401 gaaactcacc tttatgttat tgattgttca aaaggcaact agaataaaaa tatgtgttaa
    53461 aatgagaaaa aatttgattg ttggatgtat cgaataatca ctaaaaagta ggagaagaaa
    53521 atgatttttt tgcaaaaata tggtttttat tttttattat taggtgtgat tagtgattta
    53581 tcgacaccgt atatccttgg ccttttttat cccaaactga atcaaatgac taccgtaatt
    53641 agtgtgtttg gtgacgtaga tagtccagtc agacgtgctt tcttggtctg gtcagttgta
    53701 tctggacttt tttttgttct ttcgttgcct gcgttgtatc acctatttat tggaacatca
    53761 aaaacacttg caatacttgc tgttgcaaca gttggtttat atgggatagg tgattgtatt
    53821 tttactgggt tattcagtat caatacgaat gaaagctctt ggaatctatc gacttggatc
    53881 cataatatag gttctgggct aggctatgca ggctttctac ttttcccatt gttacttgtt
    53941 ctgctttatc gtcaaagtgg tcaaggtacg cttagccatt tttatcttgt tttaacagtt
    54001 attagtttac taattgcagg tctttacgga ttagcacgga ttccgtcgat tagtcagttt
    54061 gccttcttta agcaattggg cttctttcaa cgcttaagtt ttttctttaa ctatttgccg
    54121 atgatttgtt taagtttttt tcaactacga aagtagaaaa agcagtgtga agctgattaa
    54181 gtaaaagaat ttctctatgg gtaaccattc gtgtggatgt gttcgactaa gaatagagat
    54241 gaggagagcg aaaagcaagt tagtcttaac ttgttatgcg ttgtgctaaa tttcaggtag
    54301 tcattttttg aatgaataag aagaaaactt gaaagagggt gacttcatgt tagatgtgga
    54361 gcagatggaa atagaccttt ataatattat tttaaatcga aatacaagag aatgggaaag
    54421 aacacaattt gaagaggcaa aagaagacct agcaaaaatg tcgataaagc aggtgataga
    54481 aaaactagag gtcaacttgc gtccgctagc actacgagat aatttgacac cagatgcgat
    54541 ggatttttat ttaagaatca caggggagtc agcgagtgca aaggcttatg attatgccaa
    54601 acataagata acttcaccct tataccaaga acgggcgatt tttgccggtg gctgtttctg
    54661 gtgtatggtt gagccttttg aaacaaaacc aggtgtaatc tcagtgttat ctggttatac
    54721 aggtggagag accaaaaaac caaattacga tcaagtcagt ggtggatata ctgggcatgt
    54781 tgaagctgtt gagatcatat ttgatacacg tcaaataacc tatgaagagc tgttggagat
    54841 ttattggcaa atttcagacc ctacagatgc ctttggtcaa tttcaagatc gggggaaaca
    54901 atatcgttca attctatttg ttgttaatga aaagcaaagg caattggctg aaaaatcaag
    54961 agaacaactg attcaatcta accgatttag ccgtcctatc gttacagaga ttcgccaagc
    55021 aaagactttt tggccagctg aaaatcatca tcaacaattt tatcagaagc aacctaaaag
    55081 atataaaaag atcaagcatt cgcatcagct tcatttattt tttcagcgac atagatagag
    55141 gaaggttgtg gcgcaaagcg ttcagctccg acaaaataat gagcaactga caaaaatagc
    55201 ttttcatatt tttggacagt tatgaattat ttcgaggagt tgctttgaga acaccgagga
    55261 aaaggttgtg gcgcaaagca ttcagcttca agtcgcaagg aaacaggagg tatgacaagt
    55321 cttttgtcac aactcctgtt tttttactag cctttatttc catgtgaaag agaattcgaa
    55381 ttgtagaatc ttgatttttc tatggaattt tgtagagaat catcttgata attaatagac
    55441 acctaatcga attccacatt aatgatgaac taaattccgt ataaaagcgc aaacaataac
    55501 ta
//
LOCUS       DANKMN010000007        51032 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000007 DANKMN010000000
VERSION     DANKMN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            79..2244
                     /gene="acm"
                     /locus_tag="PW224_000441"
     CDS             79..2244
                     /gene="acm"
                     /locus_tag="PW224_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL2907157.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2516..3001)
                     /locus_tag="PW224_000442"
     CDS             complement(2516..3001)
                     /locus_tag="PW224_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL2907158.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3091..3436
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3481..4122
                     /locus_tag="PW224_000443"
     CDS             3481..4122
                     /locus_tag="PW224_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2907159.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4141..4776
                     /locus_tag="PW224_000444"
     CDS             4141..4776
                     /locus_tag="PW224_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2907160.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4794..5609
                     /locus_tag="PW224_000445"
     CDS             4794..5609
                     /locus_tag="PW224_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2907161.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5928..7364
                     /locus_tag="PW224_000446"
     CDS             5928..7364
                     /locus_tag="PW224_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2907162.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7553..10000
                     /locus_tag="PW224_000447"
     CDS             7553..10000
                     /locus_tag="PW224_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2907163.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10230..11297
                     /gene="rlmN"
                     /locus_tag="PW224_000448"
     CDS             10230..11297
                     /gene="rlmN"
                     /locus_tag="PW224_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2907164.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11490..12593)
                     /locus_tag="PW224_000449"
     CDS             complement(11490..12593)
                     /locus_tag="PW224_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2907165.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13145..13693
                     /gene="aac(6')"
                     /locus_tag="PW224_000450"
     CDS             13145..13693
                     /gene="aac(6')"
                     /locus_tag="PW224_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2907166.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14239..15900
                     /locus_tag="PW224_000451"
     CDS             14239..15900
                     /locus_tag="PW224_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2907167.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(15970..16317)
                     /locus_tag="PW224_000452"
     CDS             complement(15970..16317)
                     /locus_tag="PW224_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL2907168.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16401..17342
                     /locus_tag="PW224_000453"
     CDS             16401..17342
                     /locus_tag="PW224_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907169.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17343..18386
                     /locus_tag="PW224_000454"
     CDS             17343..18386
                     /locus_tag="PW224_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907170.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18392..19462
                     /locus_tag="PW224_000455"
     CDS             18392..19462
                     /locus_tag="PW224_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907171.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19452..20405
                     /locus_tag="PW224_000456"
     CDS             19452..20405
                     /locus_tag="PW224_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2907172.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20417..21427
                     /locus_tag="PW224_000457"
     CDS             20417..21427
                     /locus_tag="PW224_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2907173.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21712..22533)
                     /locus_tag="PW224_000458"
     CDS             complement(21712..22533)
                     /locus_tag="PW224_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2907174.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22630..22908)
                     /locus_tag="PW224_000459"
     CDS             complement(22630..22908)
                     /locus_tag="PW224_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907175.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23176..25185
                     /gene="metG"
                     /locus_tag="PW224_000460"
     CDS             23176..25185
                     /gene="metG"
                     /locus_tag="PW224_000460"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL2907176.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25664..26251)
                     /locus_tag="PW224_000461"
     CDS             complement(25664..26251)
                     /locus_tag="PW224_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907177.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26426..27748
                     /locus_tag="PW224_000462"
     CDS             26426..27748
                     /locus_tag="PW224_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2907178.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27903..28679
                     /locus_tag="PW224_000463"
     CDS             27903..28679
                     /locus_tag="PW224_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2907179.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28672..29241
                     /gene="rnmV"
                     /locus_tag="PW224_000464"
     CDS             28672..29241
                     /gene="rnmV"
                     /locus_tag="PW224_000464"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL2907180.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29234..30118
                     /gene="rsmA"
                     /locus_tag="PW224_000465"
     CDS             29234..30118
                     /gene="rsmA"
                     /locus_tag="PW224_000465"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL2907181.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30569..31198
                     /locus_tag="PW224_000466"
     CDS             30569..31198
                     /locus_tag="PW224_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907182.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31322..32431)
                     /gene="ugpC"
                     /locus_tag="PW224_000467"
     CDS             complement(31322..32431)
                     /gene="ugpC"
                     /locus_tag="PW224_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL2907183.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32640..33062
                     /gene="mgsA"
                     /locus_tag="PW224_000468"
     CDS             32640..33062
                     /gene="mgsA"
                     /locus_tag="PW224_000468"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL2907184.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33385..33921
                     /locus_tag="PW224_000469"
     CDS             33385..33921
                     /locus_tag="PW224_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2907185.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(33961..35724)
                     /locus_tag="PW224_000470"
     CDS             complement(33961..35724)
                     /locus_tag="PW224_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907186.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35727..37442)
                     /locus_tag="PW224_000471"
     CDS             complement(35727..37442)
                     /locus_tag="PW224_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907187.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(38221..38445)
                     /locus_tag="PW224_000472"
     CDS             complement(38221..38445)
                     /locus_tag="PW224_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907188.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            38636..41413
                     /locus_tag="PW224_000473"
     CDS             38636..41413
                     /locus_tag="PW224_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2907189.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            41494..41913
                     /locus_tag="PW224_000474"
     CDS             41494..41913
                     /locus_tag="PW224_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL2907190.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            41925..42395
                     /locus_tag="PW224_000475"
     CDS             41925..42395
                     /locus_tag="PW224_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2907191.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            42412..43191
                     /locus_tag="PW224_000476"
     CDS             42412..43191
                     /locus_tag="PW224_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2907192.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            43178..43999
                     /locus_tag="PW224_000477"
     CDS             43178..43999
                     /locus_tag="PW224_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2907193.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            44016..45065
                     /locus_tag="PW224_000478"
     CDS             44016..45065
                     /locus_tag="PW224_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2907194.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            45078..46082
                     /locus_tag="PW224_000479"
     CDS             45078..46082
                     /locus_tag="PW224_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2907195.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            46715..47734
                     /locus_tag="PW224_000480"
     CDS             46715..47734
                     /locus_tag="PW224_000480"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2907196.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            47777..48658
                     /gene="galU"
                     /locus_tag="PW224_000481"
     CDS             47777..48658
                     /gene="galU"
                     /locus_tag="PW224_000481"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2907197.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            48958..49335
                     /locus_tag="PW224_000482"
     CDS             48958..49335
                     /locus_tag="PW224_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907198.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            49941..50555
                     /locus_tag="PW224_000483"
     CDS             49941..50555
                     /locus_tag="PW224_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2907199.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT        16915 a         8807 c        10537 g        14773 t
ORIGIN      
        1 gggaggaatt gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa
       61 ataggggaga tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt
      121 attactaata taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc
      181 agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata
      241 aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg
      301 atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta
      361 atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg
      421 tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag
      481 gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt
      541 gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt
      601 tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat
      661 attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt
      721 ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag
      781 gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt
      841 aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt
      901 aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag
      961 aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc
     1021 aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa
     1081 ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa
     1141 ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat
     1201 gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa
     1261 gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata
     1321 aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac
     1381 atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa
     1441 ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta
     1501 gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac
     1561 gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt
     1621 tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc
     1681 tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc
     1741 acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat
     1801 gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat
     1861 actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa
     1921 aacagttata caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg
     1981 aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg
     2041 caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga
     2101 gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa
     2161 aaatctagtt ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc
     2221 ttacaaaaat ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa
     2281 tcaaaagaat aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt
     2341 ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt
     2401 tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt
     2461 gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca
     2521 aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat
     2581 ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa
     2641 tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag
     2701 tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata
     2761 agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca
     2821 agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg
     2881 gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt
     2941 gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca
     3001 tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa
     3061 taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt
     3121 aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat
     3181 cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg
     3241 gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg
     3301 gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa
     3361 cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt
     3421 ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag
     3481 atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa
     3541 ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg
     3601 tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga
     3661 acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt
     3721 tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca
     3781 gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt
     3841 ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt
     3901 gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat
     3961 atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact
     4021 ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc
     4081 gtaatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat
     4141 atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg
     4201 aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa
     4261 accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta
     4321 gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta
     4381 ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca
     4441 aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct
     4501 tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa
     4561 cagcgggtag cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa
     4621 ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg
     4681 aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc
     4741 ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa
     4801 aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca
     4861 aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt
     4921 tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg
     4981 atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca
     5041 ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg
     5101 gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa
     5161 acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata
     5221 ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag
     5281 aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag
     5341 cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag
     5401 actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg
     5461 agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca
     5521 atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt
     5581 ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca
     5641 cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa
     5701 tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc
     5761 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta
     5821 agcgactttt tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt
     5881 tattctaaaa gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag
     5941 aacttacctc atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag
     6001 aaggagcttg gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac
     6061 ctggaaagac atttaaagga acgaacggtg atttggcagt agaccactat catcgttata
     6121 aagaagatat tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt
     6181 ggacacggat ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg
     6241 agagattgat cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt
     6301 gggatcttcc acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg
     6361 aagatttcac tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact
     6421 ggattagttt gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc
     6481 cgccaggtgt gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa
     6541 atgcatctgt catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct
     6601 ttgcgtatac gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa
     6661 atgcagaaga cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga
     6721 ttgctgccat gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg
     6781 cagaattgct agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga
     6841 cgaatgcatt caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa
     6901 aaggttcatc agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg
     6961 tcgaacgaac aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc
     7021 gtatcacaag ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg
     7081 acaagttgac agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc
     7141 acgtcaaagc catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct
     7201 ggtcttatac agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat
     7261 atgttgatca agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt
     7321 actggtacca aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac
     7381 aagaaggtac ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga
     7441 aaaactgtaa aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta
     7501 gttcccttac ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca
     7561 aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca
     7621 gcaggaacaa atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg
     7681 ctttttaaaa ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg
     7741 aaatacttac caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa
     7801 gaatacagag ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt
     7861 cggtatctta ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa
     7921 tatcatgggg acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa
     7981 taacgttggt ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc
     8041 acagcctgga agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag
     8101 taatctggaa ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga
     8161 attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat
     8221 gaaacagcgc gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa
     8281 agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga
     8341 tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag
     8401 ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca
     8461 gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga
     8521 atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct
     8581 tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg
     8641 cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt
     8701 aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat
     8761 tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc
     8821 accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt
     8881 ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc
     8941 tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt
     9001 actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag
     9061 tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt
     9121 tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag
     9181 aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt
     9241 cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt
     9301 ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc
     9361 tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat
     9421 ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat
     9481 cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac
     9541 agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat
     9601 gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga
     9661 ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga
     9721 gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga
     9781 gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct
     9841 ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc
     9901 tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga
     9961 agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt
    10021 gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt
    10081 tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt
    10141 tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc
    10201 gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga
    10261 atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag
    10321 tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga
    10381 aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg
    10441 tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga
    10501 ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag
    10561 gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga
    10621 ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc
    10681 ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg
    10741 acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca attggtgctc
    10801 ggcacattac agtttccact agtggattag cacacaaaat caaggaattc gctaataacg
    10861 gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga
    10921 tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt
    10981 tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata
    11041 gaccagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg
    11101 tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag
    11161 ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta
    11221 aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa
    11281 aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt
    11341 ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata
    11401 aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca
    11461 gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt
    11521 tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg
    11581 atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct
    11641 aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc
    11701 ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt
    11761 acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta
    11821 ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc
    11881 aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg
    11941 tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt
    12001 ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta
    12061 gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct
    12121 tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt
    12181 ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc
    12241 gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca
    12301 gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca
    12361 tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg
    12421 acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca
    12481 gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt
    12541 tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt
    12601 tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct
    12661 gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac
    12721 cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa
    12781 taatgaattt ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc
    12841 taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat
    12901 tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc
    12961 caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta taatttttta
    13021 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
    13081 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
    13141 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
    13201 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
    13261 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
    13321 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
    13381 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
    13441 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
    13501 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
    13561 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
    13621 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
    13681 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
    13741 aatttacgaa aattacttct tgaatttttg tgaaattcca acttatttcc gaagaagcta
    13801 cttttggaac accgtgtacc agaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa
    13861 aaattttgtg aaaatggctt tctacatttt tgcataaatt tggcttaata ccgagaaact
    13921 ggcttttgaa caccgtttat tcagataagg gactgtgaca agacttttgt cacagtttct
    13981 ttttgtttat tcttttttat ctttgtatgg caataataga caatataacg aaaattattt
    14041 aatggctttt ggaagaccat ggaaaacctt gtacaaagct attttttctg aaaaattcct
    14101 tgaaacctca cgaaaaaatc attattttat gaaaaagaca aaaaaaagtg ttgatttttt
    14161 aataattatt cattaaactc ttaaacaact tcagaaaata ttaattattc tgaaaattcc
    14221 aataaagggg tacgtaaaat gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc
    14281 cttgtacttg ctggatgtta tggcggcagt tcagccacaa gcagtagttc agcaaaaagc
    14341 ggttcagcag atggaggagg cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct
    14401 gacttatcag ttgcaacaga tacaatcagt tttacagcat tgaataacgt ttatgaagga
    14461 atttaccgtc ttgatgaaga cagcaaacca cagccggcag gagctagcga gttggcagaa
    14521 gtcagtgaag acggattaac ttacaaaatc aaattacgag aagacgcaaa atggtcaaat
    14581 ggtgatcctg ttacagcagc ggattacgtt tatggctggc aacgaacagt agacccagct
    14641 acagcttctg aatatgctta cctgtatgcg ccagttgaaa atgcagaaga cattacagct
    14701 ggaaagaaag ataaatcaga actagggatc aaagcagttg gtgactatga attagaaatc
    14761 aaattgacga aacaaacacc atatttccaa tatctgcttg ctttcccatc atttttccca
    14821 caaaaacaat ccgtagtgga agaaaatggg gatgcctatg catctgcaag tgataaagct
    14881 gtttataatg gaccattcac attagaagat tttgatggac caggaacaga tacagaatgg
    14941 acttataaga aaaatgatca ttactgggac aaagatacag taaaactttc tgaaatcaaa
    15001 gtcagtgtag taaaagaatc ttctactgca ttgaacttat tcaaagatgg ccaagcagat
    15061 gacgtgatct tgtctggtga gttagcacag caaaacgcaa acgatccagc ttatacttct
    15121 gttaaagaag cgcggacaag ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc
    15181 aacaatgtga acctaagaaa agctatttct tattctatcg atcgtgaagc tttagtaaaa
    15241 caagtactag gtgacggatc agttgtctct acaggattga tcccatcaga tatggtaaaa
    15301 agtccaacaa cgaacgaaga ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa
    15361 gataaagcaa aagaatattg ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa
    15421 tttgatttga tggcttctga tgatgattca tcgaaaaaag taatcgaata tgttcaaaac
    15481 tcaatccaag aaaacttgga tggcgtaaca gtcaaaccaa cacctgttcc attctctgta
    15541 cgtcttgacc gctcaacttc gggtgatttt gacgcagtac taggcggatg ggctgctgac
    15601 tatgcagacc caagcagctt tactgatttg tttgtcacag gtaattcata caaccgtggt
    15661 cgctggagca atgcagaata tgacgaagca gttaaagcag cttcatcaac agacgctggc
    15721 gatgaacaag cccgttggga tgatttccaa aaagcaatca agatcatctc tgatgatatg
    15781 ggtgtcgttc cggtgtacca aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt
    15841 atcgttcacc atgcggcagg cgcttcatgg gattacaaat ggacatacgt tgaagaataa
    15901 tcgttaatac cgtgagcatg agaaaaaacg aggctgcggc aaatgtcgca acctcgttct
    15961 catattcact tatcctaaca aagaaaaaag catgaaaata gcagaaagag caattagtat
    16021 tccggcaatg atcagccaaa agctgtacat accgtagctg gcagacgaga gcgtggaaaa
    16081 ttctggtttt ttctttcggt tccttgttcg tgcaagatgg accgatcgct gaaaatgatc
    16141 gaagaaaccc gaagaaaaga cccataaaaa accgccgata atcagaaacg gcaatgacca
    16201 taagaataga tcattagata attgaatgag tgtaaaggaa tggttgatga gattttttat
    16261 taaaataacc agaatcagta gaaaagctac tgcgtagggg atagattttt ttttcatttg
    16321 attgctcctt tatttttggt tcattatcta ataatgtagc gaaacatctg tcagaagtca
    16381 aaagctaggg ggaaacatta gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt
    16441 tcatgatcat cacgctttgg ttaattgcaa caatcacctt tttcttgatg caattgctgc
    16501 cgggcacgcc atatacgaat caggaacgtt tgagtcctga aacaatcgag atgctaaata
    16561 aacaagtcgg tttggataaa ccagttatcg tacaatatgg gatttatctt tctaatttgc
    16621 ttcaaggaga ttttggaatt tccttccaat ttaaaaacca gccagtcgca catttgttag
    16681 ctggaagaat cggaccatcc ttgcagttag ggttacaggc aattatcttt ggaacagtcc
    16741 tagggacgat tttaggaacg atctctgcca tgaagcaaaa tacatgggca gatacatctt
    16801 ctacactcgt agcgatatta ggtcgttcca ttccaaactt tgtatttgct gttttattac
    16861 aatatatctt tgcaatcaaa ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt
    16921 acacaatctt accaacaatt gcgttggcca tgtcgccgct tgctgactct gctcggttta
    16981 tccgtacgga aatggtggaa gtactgcata gtgattatgt ggaattagca aaagcaaaag
    17041 gattgagccg ttgggaaatc gcctttaaac atggtcttcg aaatagtttg attccattga
    17101 tgactttatt aggaccgtta gctgtagcat tgatgaccgg atcactggtg gtagaaaata
    17161 tctttgctat tcctggtatc ggggaacaat tcgttaagtc aatcacaaca aatgattatc
    17221 ctacgatcat ggctgtaacg atcctttact catttatgct gatttttgtt attttgatcg
    17281 tggatctatt gtacggtcta gttgatccaa gaattcgtgt ttcagaaggg agccgaggat
    17341 aaatggaaat gataccaaag cgttatgaaa cagttgcaga tatccctgcg gatgaatttc
    17401 ttccattaca aaaaaatacc gaagaagaac gtgaacagat tactgcacct tctttgaatt
    17461 ttatccaaga ttcatggcgc cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt
    17521 tactattggt catcattttg atctctgtga tcacaatctt tgtttctcct catgatccaa
    17581 ctgcccaaaa cgtggcttat atcaatttgc ctcctcgtat tccgggaatc aatatcgatg
    17641 gattgaatgg aaaagcaatg gttggtggtg aacttgttga taaatatgct caagcaaacg
    17701 tcccttcaaa cgtaaatttc tttttaggaa cagacggttt gggacgtgat gtattaagcc
    17761 gcttatttat gggaatgaga atctcgttgc tgatcgcatt tattgctgca ttactagatg
    17821 taacgattgg tgtagcttac gggttggttt ctggtttgtt aggcggacgt gtcgataacg
    17881 tgatgcaacg ttttttggaa atcttatcag gaattccaaa cctcgttgtc atgattttga
    17941 tgttggtcgt ttttgaacca ggaatttttt caatcgtggc tgctatggcc atcacaaact
    18001 ggataccaat ggcacgtatc gttcgtgcac aaacaatgaa attaaaagat caagaatatg
    18061 ttttagctgg attgacatta ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc
    18121 cgaatatctc tagtgtcatt attattcaaa tgatgtttag tattccatct gctattttct
    18181 tcgaagcctt tttaagcttt atcggtttag gtcttacgcc tccgtcagct tcattaggaa
    18241 cgatgttgag cgaagggtac aagacattcc tgtatctgcc ttatctatta tggattccgg
    18301 cagtaacttt atcggttatc atgattggct ttaacttact tgctgatgga ttgcgtgatg
    18361 cctttgatcc taaaatgaaa gagtgaagac gatgacgaaa attttagaag tgaaagactt
    18421 agaaatctct tttgatacgt atgctggaaa ggtacgagcg attcgcggtg tcaattttca
    18481 tttgaataaa ggagaaacat tggcgatcgt tggggaatct ggaagtggaa aatcagtgac
    18541 gaccagaagc atcatgcgct tgctgtcaag caacgccaat atagatgctg gtcaaatcct
    18601 ctttaaaggt aacgatatcg ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa
    18661 agaaatagcg atgattttcc aagatccaat gacctcttta gatccgacga tgcccattgg
    18721 gaagcaagtc gctgagtccc ttagaaagca taataaagtt tcaaaaaaag agggacaaaa
    18781 agctgcatta gaattgctta aattagttgg aatacccgaa gctgagaaac gtatcaacag
    18841 ttatccgcat cagttctcag gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt
    18901 atgttatccg gaaatcctga ttgctgatga acctacaaca gcgcttgatg tgactattca
    18961 agcacagatt ttggagctgc tgaaagaaat tcagacaaaa atcgatacat caattatctt
    19021 tatcactcat gaccttggtg tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg
    19081 aggaagaatc gttgaagttg gaacatcgga agaaattttc tacaacccgc aacatcccta
    19141 tacttgggga ctgcttggtt caatgccgac attagatagt gcgaacgatc gattgtatgc
    19201 tattcctggt tctccaccgg atttattgaa cccgccaaaa ggagacgcat tctatccaag
    19261 aaatgaattc gcgatgaaaa tcgatgcaga acaagaacca ccattttttg aactatcaaa
    19321 aacacacaag gcagcaactt ggctacttgc tccgcaggca cccaaagtga caccgccaga
    19381 agaaatccaa cgtcgctggg ctatctttaa agagaaacaa aaacatactt acggtcaagg
    19441 aggaattgct gatgccaaat aaactacttg aagttaaagg attgaaacag tatttcaatg
    19501 taggaaaaaa gaatgaagta catgctgtaa atgacatcag ctttcacatt tatgaaggag
    19561 aaacattcgg tttagttgga gaatctggaa gcggaaaatc cactactggc cgtacgatca
    19621 ttcgcttgaa tgaacctact gatggtgaga tattattcga cggtcaagat ataacgaaga
    19681 tcaaagataa aaaaggtttg acgaaattcc ggcatgatgt tcagatgatc tttcaagatc
    19741 catacgcttc gttgaatcct cgtatgaaag tcagagatat cattgccgaa ggaattgatg
    19801 ttaatggact agctaaatca tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa
    19861 ctgttggatt gaatccaagc cacggtactc gttatcctca tgaattttca ggtgggcaac
    19921 gccagcgtat tggtattgca cgagcattgg cagtaaaacc gcgttttatc atttgtgatg
    19981 aaccaatttc tgcactagat gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc
    20041 tccaaaaaga acatcaattg acctatctat ttattgcaca tgatttgtcg atggttaaac
    20101 atattagtga ccgaatcggt gtaatgcata acggcttgtt attagagatg ggaacaagtg
    20161 atgaaatcta taatcacggt gttcatcctt atacagaaag tttgttatct gctattcctc
    20221 taccagatcc agaccatgag agaaaacgca gaagaatcaa atatcaacct gaaccagatg
    20281 acggacaagt acgtcaactt cgtgaaatcg caccagaaca ttttgtctat gcaactgagc
    20341 aggaagtccc atattacgca aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta
    20401 actaggaggg acatagatgc taaaagcttt taaattccgt atgtatccga cagaagaaca
    20461 aaaacagcaa ttgattcgta cttttggatg tgctcgattt acctataacc atttgctgaa
    20521 aaagcggcag aaaagttggc aacaaacagg agtagccaat ttttcgctca cacccgctac
    20581 attaaaaaaa gaatacccat tcttaaaaga agtagacagt cttgctttag caaatgcaca
    20641 gcttaatttg gatcgggctt ttcgcaatta tttcaaggga cgtgccagct tcccaaagct
    20701 taaaacgaaa aaaagtatgt ggcaatctta tacgaccaac aaccaaaccc gtacgatcta
    20761 tctggaaaat ggctacttaa agttaccaaa acaaaaagaa ctaatcaaga taaatcagca
    20821 tcggccagtt gaaggatcga ttcgctccgc tacgatttct gctcgatata atgaagaatt
    20881 ttatgtcgca ctactttgtg atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat
    20941 cgggatcgcc tatcatccga agacattgat ccaaacatct cgacctctag aagtaacttt
    21001 accaaaattt caccagacag aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa
    21061 agtccgctcc gctcaccata gaaagatcag gttagatcag gcaagcaatt atcagaaaca
    21121 aaagagaaaa gtaatggatc tttatctgaa acaaaagaac cagcgagaag attatcttga
    21181 acaattatct gggaaattgg tcaaacaata tgactactta tttgtagaat cttttccaaa
    21241 agaagaggca catgctgatt tttctattca cgactggcat aaattaataa caaaattgcg
    21301 ttataagtcc caatggtata ataaaaaatt tcttttgatc aataccgacg gagcagaaga
    21361 aagcaattcc gtaagaaaga gccaagtagt agaaaaaatg ggcagacatt cggttatcaa
    21421 gggataaaaa atgaatgaaa ctgataggag cgttagggtt agccttgtta ataaaaagtc
    21481 ttaatgactt ttgtttctag gaatttttct tttgaagaaa agctcactaa ttatgacaaa
    21541 ccaataaaac aatagcagaa aaagttcgta cgagcttttt ctgctattgt taatttttta
    21601 ttcagaaagt ttacgaatac atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt
    21661 gtttttgcgt tttttcttga atgattaggt ctgaatcttt tgctcacaac cttattcatg
    21721 gtgccccggc ttgagctgat agatttttga aggacggccg attcctacag gacgttcacc
    21781 gatctcatcg aaatattgga gcatagcttt cttaaaattg gaatgatcga tcgttttata
    21841 atcgatccct aagaatttag caaatacttt tcgagcttcc gtaatcgtga aatctttacc
    21901 taaaacttgt agaacttgcg gctcgtgttc cattttattg actacacggt tgaatgcttt
    21961 caagatgatt tgactatggt caaaagccaa agtatctttg ccagaagact ctccagtgac
    22021 taaatcaagc gagatagcca cttctccgct agataaatgg atcttgttac tatgacgttc
    22081 tagtgtgaac cagcgtactt ccttggcatc atctccggcg attagcggtt cttctccaat
    22141 aaaggctaag tagctgactg tgacgaccca gcctcgcgga tctcgattag gcgtactgaa
    22201 tgtatgcagt tgctcaatat tttcttttga aataaccaca ccagtttctt cctttgtttc
    22261 tcttaagacg ctttctccag ttgactcatc cttttggaca aagccaccag gcaatgccca
    22321 tgaatttcga taaggatgcc cttttctttg gatcaacaaa actttcagct gatcttcttc
    22381 tttgttatag cacatcagta cgatatcgac agtcaaagag ggcttgtcat attctggacg
    22441 ttcctgtttt tgataccagt taagaaattc ttcttcactt gctttatgtt cataatataa
    22501 tttttcttct tctttagaag caaaacggtt catctgacca cctccattga cagtaaaagt
    22561 actttttacc ttattgtagc agactctgaa aaaaaaccaa ggatttactt gaaaaatcta
    22621 ggttatcatt taaatatgaa aaataaatgg aagtatgaga ttgaataaaa agaaaagcag
    22681 ataaaacggc aatactttat accgctcttc acgaatggca aagaagccac aaataacgat
    22741 atatacgtca atgataaaac gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc
    22801 atggaggtta aaataaccat acatacgaat ataaaaatag ctgaatatta aatgtgctaa
    22861 tgaaagaaaa gcaatactaa gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt
    22921 cccttactaa gatagatagt ctttattata ccacgtgtga ggggagaaat aaaaagccgt
    22981 tttttagtag atatgaaaca aaacaaaaac ccgcatttat tcaacctatt catcctttta
    23041 aaggatagat tgaacgtaga tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag
    23101 ctaacagcat gcttttttta tggtatacta gcaaggaaac gaattcgcat catgcgataa
    23161 aggaggaaga gccccgtggc agaaaaggaa actttttata tcactacgcc aatctattat
    23221 ccaagcggca aacttcatat cggcaactcg tatactacga ttgcatgtga cgcaattgct
    23281 cgttataaac ggttgatggg ctttgatgtg ttttatctca ctggagtgga tgaacatggt
    23341 caaaaaatcg aaacaaaagc agaagaacta ggtgtaaaac cgcaagaata tgtagataaa
    23401 atggcggctg atgtcaagaa attatggaaa acgcttgata tcagttatga taaattcatt
    23461 cgcacgacag atgattacca taaagcagct gttcaaaaaa tatttgatcg tttattagaa
    23521 cagggagata tttacttagg tgaatatgaa ggctggtact ctgtttctga cgaagaattc
    23581 tttacagaaa cacaacttgc agaagtgtac aaagatgacg aaggtaacgt gatcggtggt
    23641 aaagcgccaa gtggacacga agtagaattg gtaaaagaag aatcatactt cttccgcatg
    23701 agcaaatacg cagaccgtct attggaatac tatgaagaac atcccgaatt catccagcca
    23761 gaatctcgta aaaacgaaat gatcaacaac tttatcaaac caggattaga ggatcttgca
    23821 gtttcacgaa caacttttac ttggggagta ccagtcaaga gtgatccgaa acatgtggtg
    23881 tatgtatgga tcgatgcatt atccaattat attactgcac taggatatgg ctccgaagat
    23941 gaaagtctat tcgaaaaata ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt
    24001 cgctttcata cgatctattg gccaatcatg ctgatggccc ttgatttgcc tctacctaaa
    24061 aaagtatttg ggcatggctg gctattgatg aaagacggaa aaatgtctaa atcaaaagga
    24121 aatgtcgttt atcctgaaat gcttgtggaa cgttatggtt tagatgcact ccgttattat
    24181 ttgcttcgtg ctgttccgtt tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc
    24241 cgtgtcaatt atgatctggc aaatgatcta gggaacttat tgaatcgaac gattgctatg
    24301 atcaacaaat attgtgatgg actcgttcct gactatgcct cacttgtcac tccatttgat
    24361 agtgaactgt ctacgacggc tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa
    24421 atggaattca atacagcatt ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac
    24481 attgatgaaa cagaaccatg ggtattagca aaagacgaag aaaagaaagc tgaattgaat
    24541 agtgtcatga tccatttagc tgaatctttg cgtatcgcag cgatattact tcagccaatc
    24601 atgacagaaa cgccagagaa gatcttcaat caactaggat tggactctga aacaatgaat
    24661 cttgaaggat tacactttgg cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca
    24721 ccaatcttcc cacgtttaga tatggaagaa gaagttgctt ttattcaaga aaaaatgtct
    24781 gaaggtactc aaacaaacga agatacagtc aaatgggatc cagaagaaac agaacttgtt
    24841 tcaacgaaag aaaaacaaat caaatttgat gtatttgaaa aagtagaact aaaagtagca
    24901 gaagtcatca actgtcaaaa agtagaaggg gcagataagt tgctgcaatt ccgactagat
    24961 gctggagaca gccaagaccg tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca
    25021 agcgaattga tcgggaaaaa agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt
    25081 ggacaaatca gtcaaggaat gatcctttct gcagaagcac cagatggctc tttacaagta
    25141 atcgaagcac caaaatccat gccaaatggc tcagaaatcg catagaaatt caaagggggt
    25201 atgacactag ttgtgtcata cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt
    25261 ccttcttatt acttgaagcc aatcatataa tacagaaaca gagaactatc caaaataaat
    25321 cctagaaaaa gaagtacgag cttcgctgat ttttgttaat ttagaataat actaatagga
    25381 atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg gcagatttta attgattcat
    25441 ctggtactct tttttattgt ccttacattc ataaagtttg aatgcaatga ctttattaca
    25501 tctatattta tatctaacag tagcgttgat gtgttaatta taagttctat cactttatgt
    25561 tcaattagtt tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg
    25621 ctttgacttc gatgattgtt ttctttattt tgtaaaaatc ctatcacttg attttatggt
    25681 aaccttttat ttttgtcttc aaacggcttg gtaagagagc agtaagcagt gcaaatggga
    25741 tcgaaaagac ataccagaaa atcgtacgtc cgataccata atgttgatag agctttgtca
    25801 acatcacgtg taacaagatc aaccctaaaa gcaaggagcc agctataaac caatgattat
    25861 cccaatgcat gacttcacta agtgccatca ctccaaaagg atagaaataa aaatagatcc
    25921 cgactaaggt catccgccag ttctttgtaa atgttccgat attcaataaa aagaaaatcg
    25981 ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc
    26041 acaagatcat cacaggacct ttgatcaatg caacgattgc gatgaacaaa gtcagcaata
    26101 ttagtttttg tgataacgtt gcaacagtca ttaatccaaa taaaattaat gtgaaccaat
    26161 gccaatggga ctgctgttgt tctttacggg aaaaatcgac ccggttggat aaataatctg
    26221 ctaatttcgg ataagaacgc ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag
    26281 gtttagtata cgaaaaataa aaaagaaata catccgtctt aagagggagt ttttcactga
    26341 aagtggagaa aaaagaatgg taaattaaaa tattctcagt tatactgaaa attataccaa
    26401 atttcaaaga agaaagagga tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt
    26461 tggcgcatta gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc
    26521 tgctgttact gctagtactc agtctacaag tttgacattg ctagcttggc ttttgggtgg
    26581 tttcttaacg atctgtgctg gattgacagt ggctgaatta gctacagcaa tcccggaaac
    26641 tggtggagct gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct ttttattagg
    26701 atgggcgcaa agcttgattt actttcctgc taatattgct gcattatcga ttatctttgc
    26761 gacacaattg acgaacttat tacaattatc taccgattat cttttgttga ttgcagtgat
    26821 caccgctgtt tctgtgacag ggttgaattt gttagggaca aaagtcggtg cttctgtaca
    26881 atcagtgacg ttgattgtta aattaatacc aattgctgtg attgtcatat ggggattatt
    26941 gaccccagga caaggaactg tccagttatt tccaatcgaa gctggaaaag atgttacttt
    27001 tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt gcttatgacg gctggttagg
    27061 tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa gatttgccta aagcaattat
    27121 tcttggtttg agttttgtaa cagtcgttta tctattgatc aattttgttt ttttgaaaac
    27181 tttgccgatt gatcatcttg ccggtaattt gaatgcagct tctgaagcat cagacgtcat
    27241 ttttggagga atcggtggaa agctggtcac tattgggatc ttgatctcgg tatacggtgc
    27301 attgaacggt tatacactaa ctgggatccg cgtgccttat gcgatggctt tagaagacga
    27361 gctacctttc agtaaacagc tgacaaagct ttcaaagaag tttacggttc cttatgttcc
    27421 tgcggttttc cagttagcag tagcttgtat tatgatgagc cttggttctt tcgacttttt
    27481 aacagacatg ttgatttttg tgatgtggct attcagttta ctgattttta tcggtgtctt
    27541 tattttaaga aaaaaagcgc cggaattgcc acgtccatat aaagtaccac tttatccaat
    27601 cgttccgatt attgcgattc tcggtgctat tttcatttta ggtatgacga tgatgacgca
    27661 aacgaaatta gcattgatcg gtataggtgt taccttgata ggtatcccag tctattatca
    27721 aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca acagacagaa aaagtacgaa
    27781 ctaggtcctc tagatcttcg tgctttttct atttcaataa aacaagttct tctattttga
    27841 tttttgctac ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa ggagagataa
    27901 acatgatatt tgattctcat acccatttaa atgctgaaca gttcaatgaa gatattccag
    27961 aaacgattca gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata
    28021 cgccaacgat tgaaaagtct ttgcaattaa gccatgacta tccaaatata tacagtatta
    28081 tcggctggca tccgacagag gctggcagtt atacgaaaga aatcgaaaat aaattacaag
    28141 aacaattgac tttaccaaag gttgtagcat taggggaaat tggcttggat tatcattgga
    28201 tggaagaccc gaaagaagtc caagataaag tatttcgcag acagattgct atcgcaaaag
    28261 aaatgaacct accaatcagt attcatacta gagaagcact tgaagacacg tatcaaatcc
    28321 taaaagaaga agatgttcga gatatcggtg gaatcatgca tagtttcagt ggtgattatg
    28381 aatgggcaaa acgatttctt gacttaggga tgcatatttc atttagcggt gtcgtcactt
    28441 tcaaaaaagc attagacgtc caagaggctg cagcaaatgt accaatcgaa cgattacttg
    28501 tggaaacaga tgccccttat ctggcaccag ttccttttag aggaaagcga aatgaaccag
    28561 gctatactcg ttatacagta gaaaagattg ctgaacttcg gaatcttccg acagaagaag
    28621 tggctgccca aacatggaca aatgcgcacc gattatttgg tctaagcgaa catggataag
    28681 atgaagatcc aagagattat agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa
    28741 gtagttgatg cagatacgat agaaacaata gggtctgcaa tcaatgaaga gatcctgatg
    28801 caaattgaac atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc tgatttttca
    28861 ggagaaaaaa taagaaaaat cattatggaa gctgttcctc aggcaaaaca tgcttttttg
    28921 ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat
    28981 gctgctattt tagaagcatt gaaaaaggtc gtgacgccag acatttccca aacggctgtt
    29041 tctgaaatca ctagacaaga ccttttagac tatggtttaa tagctggggc acaagcaaaa
    29101 gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt acacgaacgg caaacaattg
    29161 gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata
    29221 gtggaggaac aacatgagtg aatatcgtga aattgcgaca ccctctagaa caaaagagat
    29281 tttattgaaa catggttttt catttaaaaa aagtttagga caaaattttc tgactgaacc
    29341 aaatatttta aggaaaatcg tagaaactgc gcaaatagat gaacatacga acgtgatcga
    29401 agttggacca ggaatcggtg cattgacgga acaattagcc aagcatgcaa aacaagtcgt
    29461 agcatttgag atcgatgatc gtttgatccc tgtattggct gatacgatgc aaccctatga
    29521 caatgtgact attgtacatc aagatatttt aaagaccgat ttgtcaacag ctgttagaga
    29581 aacatttcat gaggagcttc cgttaaaagt cgtagcgaat ttaccttact atattacgac
    29641 accgatcatg atgcattttc ttgaatcaga tttggttgtg gatgaactag tcgtcatgat
    29701 gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga acgaaagctt atggttcatt
    29761 atcgattgct gttcaatatt atatggaagc cagtttagca ttcatcgtac caaaaacagt
    29821 atttgtccct cagccaaatg tagattcagc gattttgaaa ctgacaagaa gagatatacc
    29881 ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg acaaaagcgg catttcagca
    29941 aagaagaaaa acgctttgga ataacttgca gcattcgtat ggcaaagatg accagacaaa
    30001 agcatggtta gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc gtggggagac
    30061 tttgtcatta caagaattcg ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa
    30121 aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca
    30181 aacaaaaata gttcctcatg tttttcgttt gtttgggctt attaccgcag atgcaagctt
    30241 ttgaacaccg tttaagaaag aggttgtggg cctgccgttt aactctaagt cacaaggaac
    30301 aatttcaaaa aacagcttct catgtttttg agttagttgg tcttattact tgatgcaagc
    30361 ttttgaccac tattatgccg aacaaggaga tgtgacacaa attttgtcac atctcctatt
    30421 tttgtttctt gatcaaaaaa ttcttatact tgatactcac actcattttg tatggattta
    30481 gaaaagaata tgtgtcttga cagttattag taagtgagtt atactaagtc gacatttatg
    30541 tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat
    30601 ttctgcttta ttgattggga tgggggtcat tatcccaatg gtcatgccta aaatcatgat
    30661 tccaccagct tcctttactt tggcgtctca tgtgccatta tttattgcga tgttttttac
    30721 accaggagtt gcagtggctg ttgcgctagg tacgacattt ggtttctttt taacaacgcc
    30781 ggttatcatc gcattgcgag cattgtcaca tcttgtgttt gcgttgattg gggcttggta
    30841 cttgcaaaag catccgagta tcgttttaaa aaatggacag tttacttttg ctaattggcg
    30901 tttccaaggg ttcaattttg tgatcggtgt gattcattct ttagcagaaa tgctagtagt
    30961 cagcatcttt tactttattg gtaatatgcc tgaaacatat tattctcaag gatattttta
    31021 tacagtattc atcttgatgg ggattggtgg gcttatccac agtttagttg attataatat
    31081 tgcttatttt atagcaggaa cactcagcaa atattttgat atccccgtat ttacggcagc
    31141 aaaaaaagaa aatcggaaag agtcgattca agggtacgcg aagaaaacga catattagac
    31201 atgatctctc aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc taagatgaga
    31261 gttttttgtc ctaaaaagag ctgcaccaac atcttctggc acagctctaa cagggggcaa
    31321 tttatggaag tttgatgact tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt
    31381 tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc tcttgcatct acttttgaga
    31441 tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa
    31501 cgacaacttc tgatttcact acggcttcag gtgaagcttc aagggctact tgttcactgt
    31561 ggatgtcttc tggacgaata ccgaagataa cttcttttcc ttcataacct ttttcaacta
    31621 atactttgtt ttttccttca ggaatacgaa gcttaagccc atgtccatct gtgataacac
    31681 catttttcaa tgtcacatta aagaagttca tggcaggaga tccaataaag ccggcaacga
    31741 acatattgac gggtgtatca taaacttctt tcggtgtacc gatttgttgg ataaatccgt
    31801 ctttcatgat aacgattcgg tcagccatag tcattgcttc tgtctgatcg tgagtaacat
    31861 agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc atggctacac
    31921 gtaatttagc atctaaattt gataatggct catccattaa gaagactttt gcatcccgaa
    31981 cgattgcccg tcccaatgcc acacgttggc gttgtccacc agataaagca gctggttttc
    32041 gtttcaagta ttctgtcaga cctaagatct ctgctgcatt gtccacacgt tttttgatgt
    32101 ctgctttatc atatttacgt aatttcaagc caaaagccat attatcaaaa acagtcatat
    32161 gaggatacaa tgcgtagttt tggaaaacca tggcaatatc acggtcttta ggtgccacgt
    32221 cattcatgac ttttccgccg atatttagtt ctccttcaga gatatcttcc agtcccgcga
    32281 tcattcgcaa agtagttgac tttccacaac cggaaggacc aacaaaaacg ataaattcac
    32341 ggtctttgat tgatagatta aaatcagtta cagaataatt ttctgcattg tcatattttt
    32401 tatatacgtt tttcagagcc atttctacca taatacgttc cacactttct tttttagttt
    32461 tattaactat ttaaagtata aatgaaagcg ttaactaact tcaatgtcat gttgaacaag
    32521 aattttttct tttttttgtc aatgtgctag ttttaagctt taataaatat ccataagaaa
    32581 taaaaactga tacactggag taaataagta tgttaacaat atcaaaagga gtgtctctta
    32641 tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat gatggtgaaa ttagcaacag
    32701 cgtataaaac aatattggaa aaacatgaac tttttgctac cgggacgact gggcagagga
    32761 tcacggaagc aaccggcttg ccgatccatc ggtttaaatc cggaccgtta ggcggggatc
    32821 agcaaattgg agccatgatt tcggaggatc agttagattt ggtgattttt cttcgagacc
    32881 cgctggctgc acagccccat gaaccagatg taacagcact tatccgttta tctgatgtct
    32941 atgagattcc gttagctacg aatatcggaa cagctgaggt gctactaaga ggactggaag
    33001 ccggcttcct agattttcga agaatcgtcc atgaaataga caagcatcca ctttcatttt
    33061 gatggataag cgtatggaaa aataacgttt aatgttcgcc tatatgtagc tataaaagct
    33121 aaacaaactg ataatatgcg atataatacg aacgtattgg ataatataaa caataatatt
    33181 agtttttcgt atttgcagta aatgtgaaaa tatgctatag tttctgtggg aaaagatccc
    33241 aaaaataata cagagtagaa aataaagcgt taagtgctaa gaggatggga tgttgcctct
    33301 tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt cgctgcatga tacgatgtag
    33361 cagctcttaa aggagatgct aaaaatgaag aaacaccggt tagacgcacg tatgattgct
    33421 attatgggct tattgattgc attgatggtc actttgtccc gactggtagc aatcgagacc
    33481 ccatttatta aaatcagtgt cacatttatt ccacaagtga tcatgggcat tctttttggt
    33541 cctttttggt cagggatagg cgcagtttta gctgatttag taggaatggc cctcttctcg
    33601 aaatctgcat tctttattgg atttacattg aatgcattta ttgaaggagc aatctacgga
    33661 ttctttttct atcgaaaaga gatcacttgg aaaaatgcca ttctggctac gcttagtgtc
    33721 acgctgatca tcaacttatt tttgacacca ttatggcttg cgttgatgta ccatgtgcca
    33781 ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt
    33841 caatcaattg ccatttatta cgtaggacgt agtattcctt acaaaaaaat tttaagaagt
    33901 ttagcgatcc acgctaaata aaaaacaagc agtcttccca tcccagaaga ctgcttgttt
    33961 ttattgtgct gtgaattgtg cttgatagag ccgtcggtaa taaccatctt tttgaagcag
    34021 ctcatcatgg gtgcccattt cgacaatagc tccctgatcc atcactaata tctggtccgc
    34081 atttttgatt gtggacaggc gatgggcaat cacaaaactg gtttttcctt ccatcattgc
    34141 taagaaggct tgttggattt ttttctctgt caatgtatcg actgagctgg tagcttcatc
    34201 taagatcaac atagggggat tactgatcat cgttcgagca atcgtcaaca gttggcgttg
    34261 tccatctgat atctttaacc cttgaccgcc aatcttagta tctaattttt ccggcaaacg
    34321 ttctacaaat tcatacatat aagaggcttt tagggcatca ttgatttctg cgtcacttgc
    34381 ttccggattc ccaaaacgaa gattttctcg caatgtactg tcaaatagcc aagtatcttg
    34441 taacaccatt ccaaaacttt tacgtaatgt atcacgttgg atagagcgga tgtcatggtc
    34501 atcgactgta attgttcctt ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt
    34561 ggattttccg gcccctgttt ttcctactat agcaatggtt tgacctggat aagcagtaaa
    34621 attgaaatct tcgatcaaac gctgcttggg gtcataagaa aaagaaacat gatcaaaaga
    34681 gacttttcct ttgatatttt tgggttccaa ggcataggca tctggcaaat caggcttttc
    34741 ttctggctga tcgatcaaat caaatgctcg ttctagacca gaaaaagcgg tttgcagttg
    34801 tgtcgtgatt ccagaaagtt cgataaatgg tttagaaaac tgacttgagt agatcgtaaa
    34861 gctggaaatc acaccaacag tgattgacga accactcttc aatgccaata ggccgccaac
    34921 taaaccaatc gataaataag caaggtgatc tacaaaacga gaagacgggt ttgtcaagga
    34981 agaagaaaat tgtgcccgtt gtcctttttc atatagatca gcatttagca gttcaaattg
    35041 ctgttgacta gcttcttctt gctgaaaagc tttcacgatt ttctggttac cgattcgttc
    35101 ggtcacaaag cccgagatat ccccaataat cctttgctgt ctatcgaaat ttgtttgtga
    35161 agcacgagca acgatccaac tcacaaggaa aataattggt gtagaacaaa gaacgaccaa
    35221 tgtcagaagt ggacttagac gcatcataaa aataaacgaa agaatgatga ttgccactcc
    35281 agaaaataat tgattgaagg ccgcagaaat tgcaatggaa atattgtcca tatcatttgt
    35341 aaatcgactg acgatatttc cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt
    35401 cagatgagca aatgcatctt ttcttaattt tgcaacggat aagtaagcta accgattgct
    35461 taacacttgg atgagccatt gactgactac gttgattaaa acgataccac cgaatagaat
    35521 aagtaaatgg aacagtcgat tgaagcggac ctgtccttct ccaatcattt gatcgatact
    35581 ttgaccaatt tgtaacgtca tcacaactgt tgcaatccca ttgatgattc ctagtatcac
    35641 agctagccaa atgtcttttg cataggcagt aaggtaagga atgaatcggc ggagcgtctg
    35701 caaggataat ttttttactt tcataactat tcttcctcct gtgatgcgac gatttcctga
    35761 tattctgctg attgagcaag taattcttca tgggtaccct gagcagcaat cttaccttga
    35821 tccatcacta agatatgatg tgcttcttga atagagcgga cacgttggga gataataatc
    35881 actgtactgc caagataagt ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc
    35941 aacgcactta aagagtcatc taaaatcagt aatgctggct tggcaatcaa agctcttgca
    36001 atcgtcagtc gctgtttttg tccgccagag aaatttttcc cattttccat gacttttgta
    36061 tctaaaccat ttggaagctg acggacaaat tcttctgcct gagcgatttt cagagcttcc
    36121 caacattctt tttctgtggc gtctttctta ccccattgaa ggttttcacg aatagtcccg
    36181 gtaaatagta cagaagtttg gggaacaaga gcaatttgct gacggagatg atccagctgc
    36241 caagaacgaa catccattcc gtccattata actgtaccgg aactgacatc atagaaacgc
    36301 ggaatcaatt gtgtcaaagt cgttttcccg cttccggttg gaccaatgat tccaaggact
    36361 gtatttcgct tcagctgaaa tgtaatattt tggagagaaa gaccagaatc aggagtataa
    36421 cggaagtcaa catggtcaaa agcgataatt gcttcagaag aaaaatcggg agtagtactg
    36481 acctcagctt caagaatcga attttctgtg tctagcacct ctttcacccg atttcctgaa
    36541 gcttcggcac gtgtgaagat cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt
    36601 tgattcatat aattgatcaa agcaagaact tgtccttgtt gcaagctacc aatatttact
    36661 ttgatcccgc caactgtcag caaacagatg atcccgacat tcatgatcaa ggttgtagca
    36721 ggtgataata gggcagagat attggaaaca cgtaaataat tgttcgctag atcatttgtg
    36781 cttttatcga acttctctgt ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca
    36841 ccgctaagat tctgactaac gtgttcattg agctgatcta gtttttcttg cactttttgg
    36901 tagagaggaa cggttttttt gatgataaaa aataaaatga ttgagaaaat aggcaataat
    36961 gctaagaaaa acagacctac ttcccagtcg atcacaaaag ccatgaccac tgacccgata
    37021 cttaaaaacg gtgctctgat caataaacga atgaccattg ctaaagctag ctgaagctga
    37081 ttgatgtcgt ttgtcattcg ggtgatcaag gtatctgttc caaaatgatt caactcgttg
    37141 tgagagaaag tattgatttt tttgattagc tgattacgta actctgtacc aaatccttgt
    37201 gaagctactg aagcatagta ttggcaaatg atagcacaaa tcagaccaat cacagacata
    37261 gcgaccatcc ataagcccat ttcaatgatt tttcctcggt cattcatttt tagtccatta
    37321 tcgatcaagg aagccatcat taaaggcagg acaagttcga agacagcttc aagaaatttg
    37381 aagaacgggc ccaaaatgat ttgcttacga tagtcttttg cataacgtaa taactgaatc
    37441 atatgattcc tcctattatt tatttgtcca caattgaaaa aacaagtact accacattgt
    37501 agcttaggaa atttagaatg aaaagagaac aagccttttc ataaatgaaa gttttattat
    37561 gaagaagctt tagaaattga atagactatt ttttttaaaa aaaagaaaat gagaaaacat
    37621 gccagtctct tcattcatag gaacgtctat tttacttaat ttttctataa atgaattctg
    37681 cataaaaaat atatcaagta aatgtttata gatgaaactc tatttacttc ttttttaatt
    37741 tgttcttgca atcttttttg ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta
    37801 attgattttc aaagaaggag tatgaaaatg agaaaaatat ttattcgttt attgtgggta
    37861 ctatcttaga ggaataaata atagtggaga aaactatggt ttggcgcaga ttctatcagg
    37921 tttgatggaa cgaagacaac ctcccgacat caagtcaaaa gccacttgag attttgatct
    37981 gaaaataaaa aaagaaaaca aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca
    38041 atgggaaaaa actaagagtg gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct
    38101 ctttactctc tgaaagcatc ataactcgtt tttcctcatt atgccaataa attgttgtta
    38161 taccacatat aaggatttgt aatagctaaa aacagacagg aaagttgaaa tctgtgaatc
    38221 ttattttgta tggaaggatt tatagaacgc aaaaatcaag ataaagatga ataaaaatcc
    38281 tgtttcggct atatattgcg tatctacgcc aataaaacga gtttgttctg tcatagccat
    38341 ataaagcaaa ggcaaaaata caactacagc gagataagat ccgatgattt ctcgaaaagt
    38401 tttccaggaa gtatgtttcc tgtttttata tttatccgat ttcattgaat agtcatagcc
    38461 tccttattct ttcatatgtt gaaattccag tataaaaact ggaaggaact gtacgttttt
    38521 tagaataaaa attataaggg ataagtaggc tgctgtctat aataagatat atttttcaat
    38581 tctttattaa agattttatt gacaaaatga atccgttttc tatgatggag gaggggtgaa
    38641 taagttgttg aagaaggaaa ttttatgtta cttagagaac cagacagcct ttttcgattt
    38701 agaacatatg aacgaggtct ttacagcaca aaggttagct gatcattttg gggtaaaaag
    38761 aaacacagtc agtcattatt tgaaccagtt aaatgatgag caactactag tcaaaatcaa
    38821 ttctcgacca gtgatttatt tccacaaaga agcttttgaa aaacaatttt ttaagttacg
    38881 cacgaatttt tatttttcta tcgatgatct caaagaggaa cagccttttt tcgcgcaacc
    38941 aaaagactta ttctctttta tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca
    39001 aatcaaaaca gctctttact atccagatgg cggattacca cttttgatta caggcgaaag
    39061 tggcacaggt aaaagttatt tagttaattt ggtctatcag tattgtctgc ttcatgatct
    39121 gctagaagat tctgcaccat tcatcacttt taattgtgca caatatgcag ataatcctga
    39181 acttttgact agcaatttgt ttggacatgt taagggagcg tacactggtg cagaagaaaa
    39241 taaaaaaggg gcttttgaag cagcagatgg tgggatcttg tttttagacg aggtacatcg
    39301 cttaacacca gaaggtcagg aaaaactgtt cacctatctt gatcaaggtg tgatttatcg
    39361 tatgggagaa cctaatttgt cacgccggat caaaacacgt ctcttttttg ctacgactga
    39421 agagattact agtcatttct taacaacttt cattcgaaga atacctatcc gaatcgaact
    39481 acctgcactt aatcagcgta gtcgtaatga acggctagaa ctgatttact ctttttttct
    39541 cgaagaacaa agaaaaatgg ggagcccttt gtcagtcagt ggaccagctt tgtcactttt
    39601 aacaagtcag aaattatccg gaaacatagg agagctgaaa aatatcgtca aagtttcagc
    39661 agcaaaagca tatgctgaac agcgtgaaca gcctgaaatc catgtcacga tccatcattt
    39721 gcaaaaagaa ctacttgcgc agcctattac taaaaataca gcacagcaag gaatctatat
    39781 tacgcaagac cagaccttag agcaattaat ggagaagcaa cagcccgaac agcagcgaat
    39841 cgttaaaagt tttgagcaaa tcctaattga tttcaagaaa aataactgtc tgctatattc
    39901 ttgcgaagga aaattaaagc aagaagtgat tcaattattt gattttttgc tgtttgagac
    39961 cagtagacaa gaaaaacacg agctgctggt ttattggaca caatctatcc gagagacctt
    40021 tcgacaaatg gaaactgcct atcaaatcaa attcgatgga aacagtgtct atgccttgag
    40081 ttactatctc tatcagcgaa gcactatcaa atggttaccg gaagaacatg atattatcca
    40141 actaatcaaa gaattagaac aacaagtttc tcagtcttat ccatctagtt atcactatgt
    40201 ccaacgaatt ctggaattaa gtaagatcaa attagatatt gaagacacaa gtatggatcg
    40261 gattttactg acgatctatc taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa
    40321 agcgatgatt gctgctcacg gttatgcaac tgctagcagt atggcaaatg tagtaaatcg
    40381 tttattacaa gaggatctat ttgaatcatt tgatatgcca ctagatgtca caccacagca
    40441 gattgcttca gaaatccttg attattgcga aaacagcgat gtttcaaatg gattgatcat
    40501 attagtagac atgggatctc tgaaagaaat ccatcagttt tttaaaaaac agttatccgt
    40561 accgcttttg attttgaata atgtgacgac acctttagca atcacagtag gggaatgcct
    40621 gcaaaaaaat gtatcattag aagagatagc agaagagact gttcgacaga tccagccaga
    40681 atggcgactg ctctatccag aggaaaacaa acccaaagca ctcattacta cttgcttcac
    40741 tggaatcggt acggcggtcc acttaagtga actgctagaa aaaagcttac ctacggcttg
    40801 tcagttgaaa atcatcccat atgaatatca gcaattaaaa gataagaaaa acagtgaccc
    40861 attattttcc atatacgaag tcgtaggaat gattggcacc acagatcctg atatcaccaa
    40921 tatcgactat ctttcattag aagacctcat ctcgggggaa aagatgtctg tactagctga
    40981 atggttggac actactatga atgcttcaga gaaggaaata ttcaatcagc gcatcattcg
    41041 taatttttca ttggaaaaag tactggattc cgtgactatt ctagatacag agaaggtgat
    41101 gggagaaatc gatcatttta tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa
    41161 tcccaaaaaa ttagcgttgt atgtccatgt gagctgtctc attgaacgat tgatccgaca
    41221 aatgccaatc gaaacctatc aaggattaga gaaatggaag cagtgtcaaa aagaagggtt
    41281 atctgctatt aaatctgcgt ttagtgtcat tgaagagcat tatagtgtca tgatccccaa
    41341 ttctgagatt gcctatatct atgatatttt gagtgaagat acagagtttc tctcgataga
    41401 agaagaattt tgaacataag caagttgttg ccactaattt tggcatgcaa cttgcttttt
    41461 tagtatgtgt aagtgaaaag aggagatgaa aaagtggata gagaaattat cttagcttcc
    41521 catggaaaat ttgcttcagg gatattagat tcattagagc tgatctatgg gaaaaatcat
    41581 tctattactg tattcgattg ctatactgat gagaattttg atttagcgga aacagtaagg
    41641 cagcttttta tgaggtacaa agataaagaa atcattgttc tgacggatct ttttggcgga
    41701 agtgtcaaca acgaattctt acaatatatc aatcgggatc atgtctatct cgttgccgga
    41761 ttgaatttgc cactattgat tgagctagta tctcggttgg acgctgaagt agagacaact
    41821 gttttgatcc agcaggtgct aactgattca aaaaaaatga tccagttttg taacgagcgg
    41881 ctcaatcaga aaatagaaga agacgaattt taggaggaag aaaaatgatt gtattgacaa
    41941 gagtcgacca tcggttatta cacggacaag tagctttttc atggacacaa acaataggcg
    42001 cagactgtat tttgattgct aatgatgatg tacctacgaa tgagatacga aagacaacga
    42061 tcaaattagc caagccacaa ggagtcaaac tagtgatcaa atcaattgat gactcgattg
    42121 ccgcgttgaa aagcggggtg acagataaat ataagttatt catcgtagtg gaatcgatcg
    42181 aggatgcata taagctggca gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga
    42241 tcaaggcaaa acctggtact cgcagcatcg gaaaagcggt aaatatttta ccagaagagg
    42301 aggaacgctt gaaacgatta attgacaaac aaatagaagt agaaatccgt caagtaccag
    42361 cagataaaaa agtggatgta agagaagttt tgtaagaaag gaagaacctc aatgattgta
    42421 caagcagtct tattaggaat tgttgcattt gtcgcacaat cagaatatgc actagggaca
    42481 tcgttgcttt ctcgtccgat tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta
    42541 aaaactggga tcattatggg cgcaacttta gagttagcat ttatcggatc gttctcagta
    42601 ggtgcttcga ttccacctga tgttgtgacc ggtgggattt tagggacggc ttttgctatt
    42661 acagctggag ccggtacaga aaccgcgctt ttactaggtt tgccgattgc tacgttgaca
    42721 ttgatattga aaaatattta cttagggctt ttgattccaa ttatgaacca taaagcagac
    42781 aattatgcag aagaaggaaa ttataaagga gtcgagcata tgcacttgct tgccggtatc
    42841 ggtctttcct tgatgcttgg tgttgtggta atggtttctt atatggtggg aagcaatacg
    42901 atcggaaatc tattaaatat gattcctgac ttcgttcaaa aggggttgtc agtagctaca
    42961 gggttgattc cagctcttgg ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt
    43021 gcaccatatt ttttcttagg ttttgctata gcttcttact tagagattcc tgtgaccgga
    43081 gtagctattt ttggtgcgat tttagcagtt gtggtagtca atattatgaa tgtccgtcaa
    43141 ctaccacttc aaacaactgc aggggaggta gaagaagatg aagaattctg agatcaaaaa
    43201 cagcaaaata acgaaaaaag agctaaatca cgtattttgg cgttcttttc aaatggagtt
    43261 ttcttggaat tatgaacgtc agatgaatat ggcttatgtt tatgcaatga ttccgatttt
    43321 gaaaaagtta tatcagtcga aagaagaaat ggcagcagct ttgaaacggc atctagagtt
    43381 ttttaacacg accccacata tcgttacact gattttagga atcacggctg caatggagga
    43441 agaaaataag gatgatcctg aatttgatgt gactgcgatc gatagtatca aaacatcttt
    43501 gatgggacct cttgcagggt taggagattc tttcttttgg ggaactttgc gattgattgc
    43561 cacaggtgtg ggtacctctt tagccatgca aggaaatatt ttaggcccta tcttgttcat
    43621 tttgattttc aatattccgc acattctgtt tcgctatcta gcaacaggtt ggggatatcg
    43681 cttaggaacg ggttttttga aaaagatcca ggcaaacggt atgatggaaa gtttgacatt
    43741 aggcgcgtct attattggac tgatggtcgt tggagcaatg actgccacga tgatcaccat
    43801 caatattcct ttaaaaatcg gttcaggaga aaatgctgtg actgttcaaa gtatttttga
    43861 tgatatcgtt ccaaatatct tatgtcttgg agcatttggc atagtgtttt atctattgaa
    43921 gaaagaagtg aaaccattaa cgatcttgat cggactagct ttctttggaa tttttggttc
    43981 gtggataggg attttttaaa aaaggagcag agaaaatgtt tacgatgcaa gattatatct
    44041 atgaagaaaa ggaagtacta agtacgatct tgaaaaagaa tgatttttcg acaagagaac
    44101 atatgaaaaa aacagtgaat ttactgatct tagcaacggg ttcatcttat aacgcttgtt
    44161 tagcagcgaa gccagcattg gaatcttatg gtgatcttac tgtcgatatt caagaacctt
    44221 tttatttttt caattatgga aaactttctc cttcaatcga tactgtgatt gctgtgtcac
    44281 aaagcgggaa aagtgcttct actgttgaag cagtaaaaat gatacaaaaa caagggttac
    44341 ctgttgttgc tatcacaaat gacgtgcaaa gtccacttgc tttagaagct gcacaaatta
    44401 ttgatttagg tgccggggta gaatcagtag gcttcgttac aaaaggctat tctgcgactg
    44461 ttttacagtt actactctta gggataggaa ttggaataag taaaaataaa atcagcaaaa
    44521 aaatagaaca agactacatg caacagttgc gcaaaataat caaccattta ccagcgatta
    44581 ttcaaaaaac ggaagaattt tttgatggat atcaaagttt atttcgttta gcacagcgtt
    44641 tcgtcaccat tggatatggt ccaaactggg gaacagctaa agaagcagag acgaaattga
    44701 ctgaaacaat ccgcgttccg tcacaaggat ttgagttaga agcttatatg cacggcccat
    44761 acctggaggc agatgcttcg catttgctat tctttattga aggggaaagt gtaaataagg
    44821 aacgttcgca aaagttgcaa agatatatga gtcggtacgt tggagagact ctcaccatta
    44881 ccacaaaaaa agcaagaaac gagaaaacac ttggattagc aatagaatgt gatgaatatc
    44941 tgtctgtatt agcgcttgtc gttccgtttc aactattcgc ttataaaacc gcagtagcta
    45001 aaggtattga cttgaacaaa aagatttttg aagactttga tacagtcttg aaaagtaaat
    45061 tataagaggt ggaaatcatg ctgaaattca atgaagaaga acaaatcaaa gcaataaaag
    45121 gcgcactagc tttacgtccg caagtagaag aaatcatcga taaactttat gcggaaaaat
    45181 ttgacgctgt ctattatcta ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca
    45241 cttatatgaa tgggaaaagc aacttgcctg tctttgtaca gcatgctgca gaatattaca
    45301 ctactggaaa caaaagatta acaaaagatt ctatcgttat tttgtcctct gttaccggta
    45361 caactcaaga agtagtgaaa gcagtagaac aaatcaagaa agtaggagcg acactgattg
    45421 gatttatcga taaagcgaac agtaagttgt ctcagctatg cgattttgtg gtcacttatc
    45481 cagctcctgg aacagaacaa atcaaatttt ttatggcagc agatcgattg atgtatttga
    45541 atggagaatt cgaagcttat tcagaatact atgaacagct cgaacgttat ttaccaacag
    45601 ggttagtgga agcagagaaa aaagcagatg catttggctt atcatttgca gaaaaacatc
    45661 gacgtgatag tatgcattat tttattggag cagggaatca atggggagct gtttattcat
    45721 atgccatgtg ttactgggaa gaacaaagct ggcttccatc gaaatctatc catgctgccg
    45781 agtttcttca cgggacgtta gagatcgtag aagaaacaac cccagtcaca ttatttttag
    45841 gcgaagatga acagagagtg ttatctgaac gtgtggctaa acttttgcca aaaatttgca
    45901 gcaattatac attgatagac acaaaagact atccagtaga aggtatcagt gaaaaatatc
    45961 gcggacgtgt tttgtctttc ttattgatgc atgtagtgac acaacgcatc gatgctcatg
    46021 tggagcagtt gaactgccat ccattagaga tacgccgcta ctatcgtcag tttgattatt
    46081 aggatagact ggataaaaag aatataaaaa ataaagagag cagcgataac tgctcccttt
    46141 atttttttaa aatattggca tcaattacgt tataaaacac attttttgca ttatctacag
    46201 ttcaacttat aatctgttta atgggacatg tgggtgccaa ccattttttg ttatgcaata
    46261 gtactattta gttgttgcat ggatccgtag taaaagtcca agtacaacga gttgcgccag
    46321 tttacatagc gttttgtatc cagaatgttg actaaaacaa aagttcctat gtcaaccgtc
    46381 aacagagaca atctgtccgt tgcaggacct gacagattta gaaatgttca atctgtctct
    46441 tatgtatttt aaatcaatac tcactaggag aagtttccta acctcctaat agtaatgctg
    46501 gtagtaacat tgtcaaaact gcttcctctt aggaacaagc aaagcataac gagtgtaaaa
    46561 ataaaatgta gactattgta acatatcatt gattatttca agtaaaaacg ataaaagaag
    46621 taaacaaaca cttaaaaaca atggaaatta gtcccaactt taagaaatgt atactataat
    46681 ggaaattatc aattatttat ttggaggaat atccatgaaa caaaaaatcg cagttcttgg
    46741 tcctggatct tggggcactg ctttagcaca aacattggca gaaaacgggc atgatgtccg
    46801 tatttgggga aatgtacctg aacagattga tgaaatcaat aagtatcata caaatcagca
    46861 ttttttacct gatttgacga tcccagaatc gattatcgga tataaagaat tggcagaagc
    46921 agtggataac gcagatgcta ttttatttgt tgttccaaca aaagcgattc gttcagtcgc
    46981 acaggaactt attggtaaaa tgaatactaa gccaatcatt atacatgcaa gtaaaggact
    47041 agaacaagat acgcataaac gaatctcaga agttttggct gaagaaattc cagaagaaaa
    47101 aagacaggca attgttgtat tatctggtcc tagccatgct gaggaagtgg cagtacatga
    47161 tatcacaaca attacagctg caagtattga tcaaaaagca gcagcctatg tacaaaaact
    47221 gttcatgaat gattatttca ggatttatac aaataatgat gtgattggcg tagaaactgg
    47281 tgcagcactg aaaaatatca ttgcaattgg tgccggcgct atccatggat taggattcgg
    47341 agatgatgca aaagcagcaa tcatgactcg aggattggca gaaatcagcc gtcttggtgt
    47401 cgcgatgggt gccaatcctc tgacatttat cggtttgagt ggtgtggggg acttgatcgt
    47461 tacttgtaca agtgttcatt ctcgcaattg gcgtgccggt aacttgttag gacagggaca
    47521 taagctggaa gaagttctag aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa
    47581 agcagcagta gagctagctc aacagctagg tgttgagatg ccaattactc aaacaatcta
    47641 taatgttttg tataatggag aagatatcaa aaaagcagca aaagaaatca tgttacgtga
    47701 cggaaaaatg gagaatgaat tttctcttcg ttaaatagat gatcaatagt taatcgtcgt
    47761 ttaggaggaa cagcaagtga aggtcaaaaa agcagtcatt cctgccgctg ggctaggaac
    47821 acgttttttg ccggcaacaa aagccatggc aaaagaaatg cttccaatcg tggataaacc
    47881 aacgatccag tttattgtgg aagaagcatt ggattcggga atcgaagaca tcttgatcgt
    47941 aacgggaaaa gaaaaaagac ccattgaaga tcactttgat gcaaatatcg aattggaaaa
    48001 caatttgcgg gaaaaggaaa aaacagaatt actcgcttta gttgaagaaa cgactcaagt
    48061 caatcttcac ttcatccgtc aatctcatcc aagaggactg ggggatgccg tgcttcaagc
    48121 aaaagcattt ataggaaatg aaccgtttgt cgtcatgctg ggggatgatt tgatgaaaga
    48181 tgatatccca ttgactcaac aattgatgat ggattatgaa gaaacacaag cttccgctgt
    48241 tgccgtcatg cgtgtaccag aaaatgaaac atctaaatac ggaattattg atcctaaaac
    48301 agatagcgaa aagggtcgtt gcagagtaaa agggtttgta gaaaaacctg aaatgggaaa
    48361 agcaccaagt aatttagcca ttatcggacg ttatctactg actcctaaaa tatttgagat
    48421 attagaaact caagaaccag gagcaggaaa cgagatacag ctaacggatg cacttcagac
    48481 tcttaaccag acagaggcgg tctatgcgag agagtttaaa ggaaaaagat acgacgtagg
    48541 cgataagtta ggatatatga agacaaatat tgaatatgga cttcaacatt cggaaattgg
    48601 gacttcatta agtgcatata ttatccagtt gagtaaaaag ctaagaacag aaagttaact
    48661 ggaaataagt ggcctgctga gaatgaaagg ctatttgtta ttaaagcggc agcttgtgcg
    48721 tcatttgcgc aactgtcgtt ttagtttttt gactctagat cagttgagtc gtggaaaacg
    48781 aaagatagaa aaagactttc gtgaatagat tcagaaaata ttatgctaat ggtttaattt
    48841 ttttttaagt tgtataaaag aaaatgatta cataagagaa atgaaagcca agccagaaga
    48901 ttcatagtag aataaaaagg cgaatttttt aattataaaa aaaggtgtgt gttcactatg
    48961 ggaaaattag tcgctgttgc acagtcgctg cttgatatac tcaagtggct gatggaagga
    49021 agcaattgga tatttgtgtt gccactattg cttattagtc tatttatcat gtttcgcatg
    49081 gtcagaaaaa aagaaacgtt tctgattttt atctggtatg cagcatccgt tggggttgct
    49141 accttgatgg ttgaattggc gttaggtagt tgggaagctt gcgtatttgc tttatttttt
    49201 gctgtactgt atctaatcat ttatatccca tatatgaaaa gaaagtctcg gataggcaga
    49261 aaaggtagac atacaactac tttttctagt acagattctc tcgaacaaag tagaagaacg
    49321 agaagaagac ggtaatattt agtaggtata cgcaaaaaaa gataaaataa tggataaact
    49381 attgagatga gagatttatt taattggtgg aaaaaagctt ttttcactta tataaaagaa
    49441 tcttttttaa aggaatgctt gttgtatcgg gataagccat tccttttttt atgttattta
    49501 tatatatata aatcatacca atgttttttt gtattttctt gcttgcactt ggataagaga
    49561 ttacttacaa taaattcaga tagaaacagc tgaataaaaa aaggctccct gacccttagt
    49621 tggcgctaag aatcagggaa tgaccagtca cacacataca ccctgaccga tcattttgcc
    49681 atagtcaatg cagtgcatcg acctaattta ttgtacctaa tctagggtga aaattctacc
    49741 cctatgtaga tattttccat ggtctgcacg ttgttttatt tgctatgtca aggtattggg
    49801 cgtattcgtt caatgccttt tttgtgtttg aaacagcaaa gaccacatgt gttactgtct
    49861 aactactgtt tttttatgta cttccttaga ttaggcagct gtttctattc atgtcaagta
    49921 tagctaggcg ggagagataa atgagtttat gttattttga ctattatcac caattggaag
    49981 agaaattaag gatagaagag ccgctgatgc ttcaagctgg cacttggatc gcgaaacaaa
    50041 ttaatcaagg tggtagattg cagatatatg ctagccgcac actaagtggt gtagcatttg
    50101 aattttggga tcaaaccccg gatcttgtac caagtatatt gattgaaaat ccagccggag
    50161 gtgtctatga aacgctagaa ggtgcaggac aggcaattat tgatcagctt tctgtcaaat
    50221 cagaagacat ttttcttctt ctttcgaatg aaggaagaaa cccggcaatc atcgaactag
    50281 ctcagtggat caaagaaaat ggtcatccat taatcattgt tactggattt gatttgtcac
    50341 gaagtatcaa atcgcaacat agcagtggtt tgcgattatt tgaatttgct gatttggttt
    50401 tggacaacca cgccaattta catgatgcag tcttgacttt gccaaatcta gatccagcga
    50461 tctgcggaac agcctctctt gcaacgatct ttttgctgca gcaagttttg tattttacag
    50521 caaaacagct agtccaggac acaagtggaa aatagttatc cacatgttga caaagttcct
    50581 taaaaaaata atgttccaca taaaaatgga gcgtttgaaa aaaagaaaac gattattaat
    50641 gaggattaac aacgactact ttttttgttt tagttatttt aagatggatc atctttgatt
    50701 ttaagcttgt tttgtttaaa gtttgttatt tttataagtt gtttcacagt tatgagaaat
    50761 gagttataag aatatgtagt gtcagaaaaa taaaaagtag tggaaaaaga gacaagaaag
    50821 aatccatatg gattctttct tgtctcttaa tacacgttga tgaaagagtc ttccccaaaa
    50881 ctctttacaa agtaattata gtacatttgt tatcattatt gcaatgaagt ttacctgata
    50941 taacaaaatt gtccaacagg ataccttttt ctaatagaaa taaaatacac aaaaattgaa
    51001 ggatagataa tggatattta taagtgttta tt
//
LOCUS       DANKMN010000008        50983 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000008 DANKMN010000000
VERSION     DANKMN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            247..618
                     /locus_tag="PW224_000484"
     CDS             247..618
                     /locus_tag="PW224_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2907200.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(892..1584)
                     /locus_tag="PW224_000485"
     CDS             complement(892..1584)
                     /locus_tag="PW224_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2907201.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1587..2372)
                     /locus_tag="PW224_000486"
     CDS             complement(1587..2372)
                     /locus_tag="PW224_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2907202.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2609..4009
                     /gene="lacG"
                     /locus_tag="PW224_000487"
     CDS             2609..4009
                     /gene="lacG"
                     /locus_tag="PW224_000487"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL2907203.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4034..4351
                     /locus_tag="PW224_000488"
     CDS             4034..4351
                     /locus_tag="PW224_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2907204.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4364..6037
                     /locus_tag="PW224_000489"
     CDS             4364..6037
                     /locus_tag="PW224_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL2907205.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6051..7682
                     /locus_tag="PW224_000490"
     CDS             6051..7682
                     /locus_tag="PW224_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL2907206.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8173..9738)
                     /gene="guaA"
                     /locus_tag="PW224_000491"
     CDS             complement(8173..9738)
                     /gene="guaA"
                     /locus_tag="PW224_000491"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2907207.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9797..10720)
                     /locus_tag="PW224_000492"
     CDS             complement(9797..10720)
                     /locus_tag="PW224_000492"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL2907208.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10916..11521
                     /locus_tag="PW224_000493"
     CDS             10916..11521
                     /locus_tag="PW224_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2907209.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11829..12845
                     /locus_tag="PW224_000494"
     CDS             11829..12845
                     /locus_tag="PW224_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2907210.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12986..14287
                     /locus_tag="PW224_000495"
     CDS             12986..14287
                     /locus_tag="PW224_000495"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL2907211.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14333..14995
                     /gene="deoC"
                     /locus_tag="PW224_000496"
     CDS             14333..14995
                     /gene="deoC"
                     /locus_tag="PW224_000496"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL2907212.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15016..15414
                     /locus_tag="PW224_000497"
     CDS             15016..15414
                     /locus_tag="PW224_000497"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL2907213.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15480..16562
                     /locus_tag="PW224_000498"
     CDS             15480..16562
                     /locus_tag="PW224_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2907214.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16774..18336
                     /locus_tag="PW224_000499"
     CDS             16774..18336
                     /locus_tag="PW224_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907215.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18329..19462
                     /locus_tag="PW224_000500"
     CDS             18329..19462
                     /locus_tag="PW224_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907216.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19462..20418
                     /locus_tag="PW224_000501"
     CDS             19462..20418
                     /locus_tag="PW224_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907217.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20517..21683
                     /gene="deoB"
                     /locus_tag="PW224_000502"
     CDS             20517..21683
                     /gene="deoB"
                     /locus_tag="PW224_000502"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL2907218.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21707..22525
                     /locus_tag="PW224_000503"
     CDS             21707..22525
                     /locus_tag="PW224_000503"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2907219.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22542..23249
                     /gene="deoD"
                     /locus_tag="PW224_000504"
     CDS             22542..23249
                     /gene="deoD"
                     /locus_tag="PW224_000504"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2907220.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23547..24233)
                     /gene="gpmA"
                     /locus_tag="PW224_000505"
     CDS             complement(23547..24233)
                     /gene="gpmA"
                     /locus_tag="PW224_000505"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2907221.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24405..24986)
                     /locus_tag="PW224_000506"
     CDS             complement(24405..24986)
                     /locus_tag="PW224_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2907222.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25119..25802)
                     /gene="rpiA"
                     /locus_tag="PW224_000507"
     CDS             complement(25119..25802)
                     /gene="rpiA"
                     /locus_tag="PW224_000507"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL2907223.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26121..26534
                     /gene="rpsL"
                     /locus_tag="PW224_000508"
     CDS             26121..26534
                     /gene="rpsL"
                     /locus_tag="PW224_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL2907224.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26618..27088
                     /gene="rpsG"
                     /locus_tag="PW224_000509"
     CDS             26618..27088
                     /gene="rpsG"
                     /locus_tag="PW224_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL2907225.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27182..29269
                     /gene="fusA"
                     /locus_tag="PW224_000510"
     CDS             27182..29269
                     /gene="fusA"
                     /locus_tag="PW224_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2907226.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29441..30628
                     /gene="tuf"
                     /locus_tag="PW224_000511"
     CDS             29441..30628
                     /gene="tuf"
                     /locus_tag="PW224_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2907227.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31035..31343
                     /gene="rpsJ"
                     /locus_tag="PW224_000512"
     CDS             31035..31343
                     /gene="rpsJ"
                     /locus_tag="PW224_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL2907228.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31374..32003
                     /gene="rplC"
                     /locus_tag="PW224_000513"
     CDS             31374..32003
                     /gene="rplC"
                     /locus_tag="PW224_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL2907229.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32031..32654
                     /gene="rplD"
                     /locus_tag="PW224_000514"
     CDS             32031..32654
                     /gene="rplD"
                     /locus_tag="PW224_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL2907230.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32654..32944
                     /gene="rplW"
                     /locus_tag="PW224_000515"
     CDS             32654..32944
                     /gene="rplW"
                     /locus_tag="PW224_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL2907231.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32983..33816
                     /gene="rplB"
                     /locus_tag="PW224_000516"
     CDS             32983..33816
                     /gene="rplB"
                     /locus_tag="PW224_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL2907232.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33859..34137
                     /gene="rpsS"
                     /locus_tag="PW224_000517"
     CDS             33859..34137
                     /gene="rpsS"
                     /locus_tag="PW224_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL2907233.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34160..34507
                     /gene="rplV"
                     /locus_tag="PW224_000518"
     CDS             34160..34507
                     /gene="rplV"
                     /locus_tag="PW224_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL2907234.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34521..35177
                     /gene="rpsC"
                     /locus_tag="PW224_000519"
     CDS             34521..35177
                     /gene="rpsC"
                     /locus_tag="PW224_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL2907235.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35180..35614
                     /gene="rplP"
                     /locus_tag="PW224_000520"
     CDS             35180..35614
                     /gene="rplP"
                     /locus_tag="PW224_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL2907236.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35604..35792
                     /gene="rpmC"
                     /locus_tag="PW224_000521"
     CDS             35604..35792
                     /gene="rpmC"
                     /locus_tag="PW224_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL2907237.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35817..36083
                     /gene="rpsQ"
                     /locus_tag="PW224_000522"
     CDS             35817..36083
                     /gene="rpsQ"
                     /locus_tag="PW224_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL2907238.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36141..36509
                     /gene="rplN"
                     /locus_tag="PW224_000523"
     CDS             36141..36509
                     /gene="rplN"
                     /locus_tag="PW224_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL2907239.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36546..36854
                     /gene="rplX"
                     /locus_tag="PW224_000524"
     CDS             36546..36854
                     /gene="rplX"
                     /locus_tag="PW224_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL2907240.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36882..37421
                     /gene="rplE"
                     /locus_tag="PW224_000525"
     CDS             36882..37421
                     /gene="rplE"
                     /locus_tag="PW224_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL2907241.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37440..37625
                     /locus_tag="PW224_000526"
     CDS             37440..37625
                     /locus_tag="PW224_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL2907242.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37661..38059
                     /gene="rpsH"
                     /locus_tag="PW224_000527"
     CDS             37661..38059
                     /gene="rpsH"
                     /locus_tag="PW224_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL2907243.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38091..38627
                     /gene="rplF"
                     /locus_tag="PW224_000528"
     CDS             38091..38627
                     /gene="rplF"
                     /locus_tag="PW224_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL2907244.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38788..39144
                     /gene="rplR"
                     /locus_tag="PW224_000529"
     CDS             38788..39144
                     /gene="rplR"
                     /locus_tag="PW224_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL2907245.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39165..39665
                     /gene="rpsE"
                     /locus_tag="PW224_000530"
     CDS             39165..39665
                     /gene="rpsE"
                     /locus_tag="PW224_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL2907246.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39680..39859
                     /gene="rpmD"
                     /locus_tag="PW224_000531"
     CDS             39680..39859
                     /gene="rpmD"
                     /locus_tag="PW224_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL2907247.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39903..40343
                     /gene="rplO"
                     /locus_tag="PW224_000532"
     CDS             39903..40343
                     /gene="rplO"
                     /locus_tag="PW224_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL2907248.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40343..41638
                     /gene="secY"
                     /locus_tag="PW224_000533"
     CDS             40343..41638
                     /gene="secY"
                     /locus_tag="PW224_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL2907249.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41700..42347
                     /locus_tag="PW224_000534"
     CDS             41700..42347
                     /locus_tag="PW224_000534"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL2907250.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42538..42756
                     /gene="infA"
                     /locus_tag="PW224_000535"
     CDS             42538..42756
                     /gene="infA"
                     /locus_tag="PW224_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL2907251.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42789..42905
                     /gene="rpmJ"
                     /locus_tag="PW224_000536"
     CDS             42789..42905
                     /gene="rpmJ"
                     /locus_tag="PW224_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL2907252.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42923..43288
                     /gene="rpsM"
                     /locus_tag="PW224_000537"
     CDS             42923..43288
                     /gene="rpsM"
                     /locus_tag="PW224_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL2907253.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43316..43705
                     /gene="rpsK"
                     /locus_tag="PW224_000538"
     CDS             43316..43705
                     /gene="rpsK"
                     /locus_tag="PW224_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL2907254.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43785..44723
                     /locus_tag="PW224_000539"
     CDS             43785..44723
                     /locus_tag="PW224_000539"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL2907255.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44751..45131
                     /gene="rplQ"
                     /locus_tag="PW224_000540"
     CDS             44751..45131
                     /gene="rplQ"
                     /locus_tag="PW224_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL2907256.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45403..46242
                     /locus_tag="PW224_000541"
     CDS             45403..46242
                     /locus_tag="PW224_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2907257.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46218..47087
                     /locus_tag="PW224_000542"
     CDS             46218..47087
                     /locus_tag="PW224_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2907258.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47077..47874
                     /locus_tag="PW224_000543"
     CDS             47077..47874
                     /locus_tag="PW224_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2907259.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48077..48823
                     /gene="truA"
                     /locus_tag="PW224_000544"
     CDS             48077..48823
                     /gene="truA"
                     /locus_tag="PW224_000544"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2907260.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            49488..49725
                     /locus_tag="PW224_000545"
                     /pseudo
     CDS             49488..49725
                     /locus_tag="PW224_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49869..50645)
                     /locus_tag="PW224_000546"
     CDS             complement(49869..50645)
                     /locus_tag="PW224_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2907261.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            50768..>50983
                     /locus_tag="PW224_000547"
     CDS             50768..>50983
                     /locus_tag="PW224_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2907262.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIIT"
BASE COUNT        16171 a         9273 c        11062 g        14477 t
ORIGIN      
        1 atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta gcttattgat
       61 tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa cactcagaga
      121 acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc tggttgggaa
      181 aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga gtatgccgat
      241 aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca atcccagtca
      301 ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc ttcagggatt
      361 agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa aattgtacgc
      421 atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt taatgatact
      481 tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga aacgtttatt
      541 tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg tggagaggaa
      601 ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca aaaaagtact
      661 cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt taatgttaat
      721 tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa gatacactgc
      781 tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc agttttttat
      841 aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg tttaagaatt
      901 aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga tggttccatc
      961 tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat caattgcaat
     1021 aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat cttcgataac
     1081 aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa tatccggttg
     1141 cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa accagcgggc
     1201 taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg tggcaatcgt
     1261 taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt ttgtcaactg
     1321 aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt cttctgacaa
     1381 tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa tctccgtatt
     1441 cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt ttgtatgatc
     1501 atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca ttgttccgtt
     1561 gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc ggttgaatca
     1621 gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga gatctggcga
     1681 tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc atctcgattg
     1741 aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc gatctgctgt
     1801 gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc atttgaactg
     1861 ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg tccgacaaat
     1921 gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc atctgcttga
     1981 gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt gttcagtaaa
     2041 tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc tccctcgtga
     2101 atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag agtggctttt
     2161 tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc tccatggaca
     2221 cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt catatcagaa
     2281 acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt gaccatttct
     2341 acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt aaggataatc
     2401 tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt tagggtggtt
     2461 tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt atttgtgttt
     2521 gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa agagcgaaaa
     2581 ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt ttatttttgg
     2641 cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca aaggcgctgt
     2701 tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg caagtgattt
     2761 ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa atggtctaag
     2821 attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa atccagaagg
     2881 aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa cgccgtttgt
     2941 gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt ttttgaatcg
     3001 agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat tcaaagaagt
     3061 caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat atttgttagg
     3121 tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc ttcataatat
     3181 gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc cgggtgaaat
     3241 tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg aagataagca
     3301 tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta cctatttagg
     3361 ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa atcaggccag
     3421 ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc gtaatgacta
     3481 cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag aaaatgcaat
     3541 ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg gaatcggaga
     3601 acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt atccagaagg
     3661 tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca aaatctatat
     3721 cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta tggatcagcc
     3781 gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa cagccggggt
     3841 aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga caaatgggta
     3901 taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt acccaaaaga
     3961 atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat aagtgataat
     4021 ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga tcgttgcata
     4081 ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac aaggaaactt
     4141 tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg cacataattc
     4201 gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg gattcatttt
     4261 tatccatggg caggatcatt taatgaccac tttattgctg agagatctga ttcaagactt
     4321 tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg gaatcattaa
     4381 acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt atttaggagc
     4441 cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca ttttcatttt
     4501 gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct ccacatggct
     4561 atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag caaccactgc
     4621 tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa tcaacaccac
     4681 ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc cgattgaagg
     4741 tggattttca acggaatata tggggacaaa gggactactg gcttcctttg tcgctgcatt
     4801 tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta aaatgcctaa
     4861 agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct ctttttcagt
     4921 atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct catttgcaga
     4981 agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact taggaatcgc
     5041 tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac cgtcgatcgt
     5101 ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc aattgttcaa
     5161 ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg taggaactat
     5221 gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg ctaagtctaa
     5281 gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg tcaacgaacc
     5341 attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat ttttgttaac
     5401 acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca tgaacagctt
     5461 tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag gaacgggcat
     5521 gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc tgatctatct
     5581 accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa aagcagaggt
     5641 gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg tggcagcgag
     5701 tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg ctggcgcagg
     5761 gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga aagcaccgat
     5821 cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt ttgatatggt
     5881 ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca ccgatcgctt
     5941 aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga gagatcctga
     6001 aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata atgaaaatgg
     6061 gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg ggggctgttt
     6121 caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt tcgatcctgt
     6181 cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa gatgccttag
     6241 agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag gatccttata
     6301 atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg attgaccttg
     6361 caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac agtgattttt
     6421 gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc tttgaagaaa
     6481 tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag cagctggacg
     6541 tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc tggccttatg
     6601 gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt ttgaagtctg
     6661 gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc cacttagctg
     6721 atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt cgaggtgtgt
     6781 catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg aatgaacttc
     6841 aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta gttgaaacag
     6901 cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt gaagccgaag
     6961 cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg ctggatcttg
     7021 ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat tgggaacctc
     7081 tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat ctcagtgttc
     7141 aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct ggaaacgcct
     7201 tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt aaaaatgcaa
     7261 gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg tttcaacaag
     7321 gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac tacaaactaa
     7381 ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt ggcataagca
     7441 aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat actaatcagt
     7501 tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa acaagtgaat
     7561 ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa attggggttt
     7621 atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg gtgaaacaat
     7681 agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc gttagcgtag
     7741 aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac gatttccagt
     7801 catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt atgctaaaga
     7861 gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc agataacata
     7921 aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt gagcacttag
     7981 atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt tttatgataa
     8041 gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc ttatttttaa
     8101 tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc ccctccatat
     8161 tatgttggct ctttattccc actccactgt tgctggtggt ttagaagtga tgtcatacac
     8221 gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt gcaagacttc
     8281 ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag cacggatacc
     8341 gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga tccctggaag
     8401 aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt cttcacgcaa
     8461 gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc caagaacacg
     8521 aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag gcatgcctaa
     8581 ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt cgatcaattc
     8641 aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg tctgtgctgt
     8701 ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga aagagacgcc
     8761 ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat
     8821 gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga agcgatctct
     8881 tgcattgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca ttacttgttc
     8941 cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat
     9001 cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa gaccaagcaa
     9061 gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga taaagttatc
     9121 cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca gcaattcgat
     9181 tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat tacgtgttgt
     9241 gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa agccttcagg
     9301 aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg gtgtttcttt
     9361 aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt cgcggttttt
     9421 agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat agcaaatccc
     9481 taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat tctcatcata
     9541 tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt ttacttcttc
     9601 tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa tacgtcttgt
     9661 gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa cgttcgtcat
     9721 atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct caaaatagtc
     9781 aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata tgattttcag
     9841 ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt
     9901 tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca cgatctccga
     9961 tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt aacgcaccaa
    10021 aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag acagaaaaat
    10081 caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct
    10141 caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg atatcataca
    10201 catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca cggttcaaga
    10261 aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc atgattccac
    10321 gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc
    10381 ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta ctttttccaa
    10441 cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat ttatgaagaa
    10501 ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa atcagatgag
    10561 caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc aaacttttga
    10621 tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat tctcgccatt
    10681 cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc tgcgttctcc
    10741 catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa gagagattgc
    10801 cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt gacttgcgca
    10861 cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg aagacatgac
    10921 aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat gggcttttga
    10981 gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac gtgaaacagt
    11041 ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac ctgatgaagg
    11101 aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg catttgcgac
    11161 acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa
    11221 cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct atgaagcagt
    11281 tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt
    11341 tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta cactgacaat
    11401 tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg
    11461 taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg tgaaagaata
    11521 actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg
    11581 tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt tcttcaaaaa
    11641 gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata cagaacttgt
    11701 atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat actttccttt
    11761 tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga tagaagggag
    11821 ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta tcagtaacaa
    11881 ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca agaaaaaaag
    11941 tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca aatgtgatca
    12001 atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc ttcttctcca
    12061 aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc atgttatgca
    12121 attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc caccgctcag
    12181 tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca ttagacagcg
    12241 gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt cgggataacg
    12301 gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg tttattgaaa
    12361 atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa ttagaagaac
    12421 gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat gggaaatttg
    12481 ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac
    12541 ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga tgctatcagg
    12601 cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga ttcgatggat
    12661 taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca agttttgata
    12721 ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa agaaaagttc
    12781 cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg
    12841 gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag tgcttttttt
    12901 atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat gtataatacc
    12961 gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa aaaagaggga
    13021 tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata cgaatggaaa
    13081 gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag acatgacaaa
    13141 tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca tcgatttgag
    13201 ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg acacgaccac
    13261 attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa tgtctggacg
    13321 cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg gatttcagat
    13381 cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg
    13441 tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg acgtcacagc
    13501 gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg
    13561 agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga agaacatcga
    13621 agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta accgagaaac
    13681 agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa atagtctaga
    13741 agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag tagaaatgtg
    13801 ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa ccgatgaagc
    13861 aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat
    13921 gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa ttttgactgc
    13981 acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa
    14041 tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag aagaagatat
    14101 tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga caaaaggtga
    14161 gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg aattattata
    14221 taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg acatcatcac
    14281 agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag aaatggaact
    14341 aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag ctgtgatgca
    14401 aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc cagcatgggt
    14461 tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag taatcgggtt
    14521 tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg ccatcaataa
    14581 cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca
    14641 acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa
    14701 agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt gcgaaatagc
    14761 aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa
    14821 ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc
    14881 cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg ctacaagaat
    14941 cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct attaattttt
    15001 tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc tattgaagcg
    15061 ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct cgtaacggaa
    15121 tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt aacgaattgt
    15181 gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt tacgcatcta
    15241 gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg
    15301 gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg tgtgacgaag
    15361 gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct ataactcatg
    15421 ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc tttacagaaa
    15481 tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg ttatttttag
    15541 gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca tcagacacaa
    15601 caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc aaccagtcag
    15661 cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga aatgatggct
    15721 tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac caagcattga
    15781 atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg atccaagaac
    15841 aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc gatggattag
    15901 ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag
    15961 cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa
    16021 tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa
    16081 aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat aaaggacgtt
    16141 caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct tcaggcggta
    16201 caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac aaaaaagttt
    16261 gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta aacggcgaaa
    16321 agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta gaagatttgg
    16381 cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat ggattgaaag
    16441 aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa gcagttgatg
    16501 aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct gaagaaaact
    16561 aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt tgattagaac
    16621 aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa caactccctg
    16681 aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt caatcgttgc
    16741 cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa
    16801 atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat caatttacaa
    16861 gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa atctacattg
    16921 atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat gcgtgggaaa
    16981 gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat ggtccatcag
    17041 cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg aagcgagcct
    17101 agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa
    17161 cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt tggaatggaa
    17221 caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat ttttgatgag
    17281 ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta
    17341 gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat caaagcagta
    17401 gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac
    17461 gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt caaaacgatg
    17521 aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa
    17581 aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt
    17641 ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt gactggtttg
    17701 cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa taaaaaaccc
    17761 cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa atacgggtta
    17821 gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca aaaaccttac
    17881 agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga attgatcgaa
    17941 gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc aggcggaaat
    18001 cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca
    18061 aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa
    18121 caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga aattttaaat
    18181 gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa
    18241 gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga agaagcacga
    18301 aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt
    18361 attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat tgatggctgc
    18421 ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc ttggttcgca
    18481 aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag cattaggttt
    18541 ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag cactttgcgg
    18601 gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag ttgttttgct
    18661 tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg ttccaggttt
    18721 acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga actatatctt
    18781 attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc gctcaagttt
    18841 ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc
    18901 ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc ttattttcgt
    18961 ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg gattgaatcc
    19021 ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt cgatggtcat
    19081 ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt
    19141 ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt ccttgcttgg
    19201 ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt tgaagatcgg
    19261 tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc
    19321 tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt
    19381 ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa
    19441 ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat ccttacttct
    19501 acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt ttctgaacga
    19561 agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt tagttccatc
    19621 gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat cgctatttta
    19681 gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac agtcagtttg
    19741 cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc tttaggtgtt
    19801 ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca gaatctgggg
    19861 tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt ctttaaaaat
    19921 acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt gatcatcttc
    19981 aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc tgccgacaca
    20041 ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg cttgttaggc
    20101 ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc tggtacgatc
    20161 gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa tcctttaggg
    20221 gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat tggcgcacaa
    20281 ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata tttgctgaca
    20341 atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa cgggaagaac
    20401 tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg aagtgttttt
    20461 cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga ataatcatgt
    20521 ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca ccagatgccg
    20581 ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa gcaggattga
    20641 acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa ggtgtggaat
    20701 cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta gggaaagata
    20761 cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt cgtgtgtttc
    20821 ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg
    20881 tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa caccaaatga
    20941 aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc gcggcacatg
    21001 aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt gatataacaa
    21061 aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag
    21121 acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga cacacagtcc
    21181 ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc aacgatattt
    21241 tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat ggcgttgacc
    21301 agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat ttagtagatt
    21361 ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat
    21421 ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg ctaatgatta
    21481 cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga gaatatgtac
    21541 cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa ggatattatt
    21601 cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa
    21661 gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga ccaagttaag
    21721 cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag aaattgattt
    21781 tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag atgctgtaat
    21841 gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa
    21901 gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga gattccacta
    21961 ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg ccggactagg
    22021 ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg
    22081 agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt tgatcggtcc
    22141 gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata cgcctgacta
    22201 tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag aaggcgtata
    22261 tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt ctcggacaat
    22321 aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg
    22381 attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc aagcgaatct
    22441 gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta aagcattggt
    22501 taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg catattgaag
    22561 cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg cgagctaaat
    22621 atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc ggtatgttag
    22681 gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga atgggaatgc
    22741 catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag aaattgattc
    22801 gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta gtactagcac
    22861 aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt cattttcctc
    22921 caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa aagggctata
    22981 cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat ttgacagaga
    23041 cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg gctctttatt
    23101 acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc gaccatttga
    23161 tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa atgatcgaag
    23221 taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc ctgcattaag
    23281 taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg gacttattat
    23341 tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc gtttaactct
    23401 gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt
    23461 gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa agggactgtg acaaatcgtg
    23521 tcacagtccc ttttttatag tgttttttat aggtagtatt ttttagcaac tgttaagtca
    23581 tcatttaatt cataaacaag tggttgacct gttggaattt caagatccat gatgtcttcg
    23641 tcagagatac cttcgatatg tttagctagg gcgcgtaatg agttaccatg tgcagctact
    23701 aaaacagttt tgttgtctag taatgctgga gcaatttcat cttgccagaa tggcaaagcg
    23761 cgttctaatg tcactttcaa gttttctcca ccaggaatat cgcgtttgtc taacattgcg
    23821 taacgacggt catttgctgc agaaccttcg tcagtttctt ccattaatgg aggaagagta
    23881 tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt atttttcagc tgtttctttt
    23941 ttgttcaaac cttgtaattt accatagtga cgttcgttta agcgccatga tttgatttgt
    24001 ggtacccaaa gttgatctga atattctaag atcaagttac atgttttgat tgcacgagtt
    24061 aaaacagaag tgtaagcaac atcaaattcg attccagctt ctttgatttt gcgtccgcct
    24121 tcttttgctt cttcgattcc ttctggtgct agatttacat cagcccaacc agtaaattgg
    24181 ttcaatgcat tccattcact aagaccgtga cgagaaaaaa ctaattttgg catgtttagc
    24241 tctcctttta agataggttt atatttctga aattttcagt tctgttttat tgtacaattt
    24301 tttggaagag atttccatcg aaaaattaga taagattcta tgagaaaaca agtagagaat
    24361 ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta ggtattagat gcataattgc
    24421 tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga aacaccgcat
    24481 tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg gatttttttg
    24541 atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg agcttcatct
    24601 aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag gttttccatt
    24661 aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag actaatcaag
    24721 tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt agatttgcct
    24781 aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc gatcactgac
    24841 tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg gaaaatttct
    24901 tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc atttatggtt
    24961 gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt ttctaactgg
    25021 atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc gcaaattttc
    25081 cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg gattgcgtca
    25141 agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt taggaaaagc
    25201 ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc tggctgttcg
    25261 atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa gacttctccg
    25321 ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg aagtagctgt
    25381 tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt atcgactaat
    25441 ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat tttctcgaaa
    25501 agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc atcagcaccg
    25561 tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat tcctaatttt
    25621 tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa accttcttca
    25681 cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt cccgagacct
    25741 acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg ttttaaattc
    25801 atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat tttagcgtga
    25861 tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa aaaaaattgc
    25921 atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt tatctacagg
    25981 tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga tgcagacatt
    26041 gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag acgcaaatcg
    26101 tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa cctcgtaaat
    26161 caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc tttaagaaaa
    26221 ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg ggtacaatga
    26281 cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg tcaaacttaa
    26341 ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac agtgttgtat
    26401 tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc gttcgcggtg
    26461 ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac ggaactaaaa
    26521 aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta tatagaagta
    26581 attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag gtcctgttgc
    26641 aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc gcttgatcaa
    26701 ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac attatctata atgcctttga
    26761 cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag caatgaaaaa
    26821 cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact accaagtacc
    26881 agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag taaactacgc
    26941 tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca tggatgctgc
    27001 aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg ctgatgccaa
    27061 ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct tttataagtc
    27121 gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag aaacaaggag
    27181 aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg cccacatcga
    27241 tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa tccataaaat
    27301 tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac aagaacgtgg
    27361 tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa tcaacatcat
    27421 cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc gtgtacttga
    27481 tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg aaacagtttg
    27541 gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa tggacaagat
    27601 cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag caaacgctca
    27661 cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg accttgtaac
    27721 aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa cagaaattcc
    27781 tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag ctgtagctga
    27841 aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg aagctgagtt
    27901 aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac tatgtggttc
    27961 agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt acttgccatc
    28021 accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag aagtagaacg
    28081 tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga ctgacccatt
    28141 cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag gttcatacgt
    28201 acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga tgcatgccaa
    28261 ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg ttggtttgaa
    28321 agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc ttgaatccat
    28381 ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag ctgaccaaga
    28441 taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc gtgctgaaac
    28501 aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact tagatatcat
    28561 cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc ctcaagtatc
    28621 ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac gtcagtctgg
    28681 tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag aaggtgctgg
    28741 tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca ttccagctgt
    28801 tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc cattagtaga
    28861 tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg aaactgcctt
    28921 ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc cagtaatcct
    28981 tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg atgttatggg
    29041 tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg gcggccaaca
    29101 agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa ctcttcgttc
    29161 tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag atgtaccaaa
    29221 atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat ttcttaaaac
    29281 cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag tttcgtgtaa
    29341 aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata cctatcagac
    29401 aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag aaaaatttga
    29461 ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg gtaaaactac
    29521 attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag ctatggctta
    29581 cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct caacagctca
    29641 cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag gacacgcgga
    29701 ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct tggtagtttc
    29761 tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc gtcaagttgg
    29821 tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg aagaattact
    29881 agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc ctggtgacga
    29941 tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt catacgaaga
    30001 aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag aacgtgacaa
    30061 cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac gtggtactgt
    30121 tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg aagttgttgg
    30181 tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc gtaaattgtt
    30241 agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg cacgtgaaga
    30301 catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata caaaattctc
    30361 tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat tcttcactaa
    30421 ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg aattaccaga
    30481 aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat taatccaccc
    30541 aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta cagttggtgc
    30601 cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa gcaacattta
    30661 ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct tttttattta
    30721 ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga aataaaagag
    30781 tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga aaaagccttg
    30841 ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg aattgcacag
    30901 acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc ccggacgctt
    30961 tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc aaaataggcg
    31021 aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg tatgaacacc
    31081 gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact ggagctagcg
    31141 tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc cgtgcgactc
    31201 ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt cttatcgaca
    31261 ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta ccatctggtg
    31321 taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta ctcatgacca
    31381 aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct ggtgaattaa
    31441 ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa acaatggaaa
    31501 cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt ttatcaaaca
    31561 aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc attcgtgaat
    31621 tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta gatgttttcc
    31681 aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc caaggggtta
    31741 tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac catcgtcgtc
    31801 ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt cttgctggcc
    31861 gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc gatgcagaaa
    31921 gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta attactatca
    31981 aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga atgccgaatg
    32041 tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac gaagaaatct
    32101 tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa cgtgcgtcat
    32161 taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc ggacgtaaac
    32221 catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca ccacaatggc
    32281 gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa cttcctaaaa
    32341 aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa aacaacttgg
    32401 tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa caagttcttg
    32461 ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac gactttgctg
    32521 ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat gtgagtgtgc
    32581 tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact caaattgagg
    32641 aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca ctgaaaaatc
    32701 aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg caaacaaaac
    32761 tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg tgaacatcgt
    32821 gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa aaaaacgtcg
    32881 caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg aagctgctga
    32941 ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat taaaaagtac
    33001 aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga aatcacaaca
    33061 tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg taacaacaac
    33121 ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg cgtgatcgac
    33181 ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata tgatccaaac
    33241 cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta catcttagca
    33301 cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga tatcaaagtc
    33361 ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa cattgaaatg
    33421 aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca agtacttggt
    33481 aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat gatcttagct
    33541 gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat caacatcggt
    33601 aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag cgtaatgaac
    33661 cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg taaagcacca
    33721 gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa agctaaatca
    33781 gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg tgtcgcacat
    33841 tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt cgatgagcat
    33901 ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat caaaacttgg
    33961 tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt ttatgatgga
    34021 cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt aggtgaattt
    34081 gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa acgctaattt
    34141 gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa ctgcaaaaac
    34201 agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag gtaaaagtgt
    34261 cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa tcattgaaaa
    34321 agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg ttgaaaactt
    34381 ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc gtcctcgtgc
    34441 aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag tcgtatcaga
    34501 aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg cgtgtaggca
    34561 tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag ttcttacacg
    34621 aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct gtatcaacag
    34681 ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct aaaccaggta
    34741 tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta aacaaattaa
    34801 ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta gatgcaaaat
    34861 tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt cgcgctcaaa
    34921 aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact caagtatctg
    34981 gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga actgttcctc
    35041 ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca acatacggaa
    35101 aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa aaaaacactg
    35161 agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc gtgaattccg
    35221 cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg aatacggttt
    35281 gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc gtatcgcaat
    35341 gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc acaaatctta
    35401 tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg aaggatgggt
    35461 tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg aagaagtagc
    35521 tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga tcgtaaaacg
    35581 tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta accactgccg
    35641 aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga ttccaattag
    35701 caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg attgcacgca
    35761 tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt aagcgtatga
    35821 ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac aaaatggata
    35881 aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc aaacgcatga
    35941 actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt ggcgacatcg
    36001 tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg ttggaagtag
    36061 tcgaagaagc agttattatc taataacacg agattttcct atatagattg aatgcaaaat
    36121 ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc gctgataact
    36181 caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt aaaactgcta
    36241 acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg gttgtcaaaa
    36301 aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt cgtacagacg
    36361 gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat aaaagccctc
    36421 gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac ttcatgaaga
    36481 tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg aggtgcgaaa
    36541 cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa gacaaaaaca
    36601 aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc gaaggtgtaa
    36661 atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga attgttgaga
    36721 tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt gtagctggcc
    36781 gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa accggtgaag
    36841 ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc ctaaaagaaa
    36901 aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc tctgttatgc
    36961 aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg gtatcaaacg
    37021 ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa aaaccaatga
    37081 ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg ccaattggtg
    37141 caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg gtttctgttt
    37201 cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac ggtcgtggaa
    37261 actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat gatttagtag
    37321 ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat gaagaatctc
    37381 gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag gcgaactacg
    37441 tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca acacaagctt
    37501 atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc catctttgcc
    37561 gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag aaagctagct
    37621 ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga cagatccaat
    37681 tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt ctttagaagt
    37741 acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag gtttcgttcg
    37801 tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc taaaatttgg
    37861 taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag gtttgcgtgc
    37921 ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg caatcatttc
    37981 gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg gcggcgaagt
    38041 tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa gtgagccgta
    38101 tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac ggaaacaacg
    38161 ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat atcaaaatga
    38221 atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa atgaaaacaa
    38281 ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc gaaggtttcc
    38341 aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga tcaaaacttg
    38401 tattgaacgt tggttactca catccagtag aaatcacacc accagctgga gtaactgttg
    38461 aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa gttggcgaat
    38521 tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa ggtattcgtt
    38581 atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg tttagaaaaa
    38641 ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt caaaaaaata
    38701 catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt gaaatagtag
    38761 tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa caaaacacgt
    38821 caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg cccacgcttg
    38881 aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt agcgggtgta
    38941 acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa aacagaagca
    39001 gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat taaagtagta
    39061 gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc tgaagctgct
    39121 cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt tatatcgatc
    39181 caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca aaagttgtta
    39241 aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa aacggacacg
    39301 tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa gcaattgaag
    39361 atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca cacgaagtaa
    39421 tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt tctggggttg
    39481 ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat atcacttcta
    39541 aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa ggtttgaaac
    39601 aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa gaattaatcg
    39661 gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca gtattatcgg
    39721 acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga ttaacagttc
    39781 agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt ctcacctagt
    39841 ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga ggtgccaaac
    39901 ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt aaccgtgttg
    39961 gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa ggacaaaaag
    40021 ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca ttgttccgtc
    40081 gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc attaatcttg
    40141 atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta gttgaagctg
    40201 gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa ctaaacaaga
    40261 aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt gaagcggctg
    40321 gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta aagtaaaaga
    40381 cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc taggtgcaca
    40441 tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt tgccattttt
    40501 caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt tctcaatggg
    40561 ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg acatcgtgcc
    40621 tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga atcaagcgac
    40681 tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg ctggattcaa
    40741 tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg taacgatcgg
    40801 tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga tcacagaaaa
    40861 aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta gattgcctga
    40921 aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag acatatggaa
    40981 atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga cattcgtaac
    41041 atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg tagctggcgc
    41101 gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc cagttatctt
    41161 tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg gtagattcgg
    41221 ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag cacctggtgc
    41281 tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg ttcaagtaaa
    41341 ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa gcgtacgccc
    41401 tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta cagttggtgc
    41461 agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt gggatcttcc
    41521 acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag ctttagaaac
    41581 tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca ttgagtaaga
    41641 aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga gggaaaacaa
    41701 tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct gaaaaaatca
    41761 ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca gcgatgcaaa
    41821 acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca ttagttcctg
    41881 atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca gagaaaggct
    41941 tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac gcaatgctaa
    42001 aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa gaaatcttgg
    42061 tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac cataaaattt
    42121 tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa ttctatcaaa
    42181 gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc aaaaatagtg
    42241 aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat ggtgctcgtg
    42301 aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac gattgcccgt
    42361 agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat agaatatgtc
    42421 agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag gtgggaactg
    42481 cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt actgtgcgtg
    42541 gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc gaatgcaatg
    42601 tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg taaaattcgt
    42661 atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc tccatacgat
    42721 cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa actacaggag
    42781 gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt aaagtaattc
    42841 gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa caacgtcaag
    42901 gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc ctcgtgataa
    42961 acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg ctaaaaagat
    43021 cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga atgaacaaac
    43081 agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc gtcgtgaagt
    43141 gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc gtcatcgccg
    43201 tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta aaggcccagc
    43261 tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa acttcatggc
    43321 agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag gtattgcaca
    43381 tatccattct acattcaata acactatcat catgatcact gatactcacg gaaatgcatt
    43441 agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa ctccttttgc
    43501 cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat taaagactgt
    43561 tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt cattacaagc
    43621 tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata atggtgcccg
    43681 ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta tcagtaacgt
    43741 cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt gagttcgaaa
    43801 aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc gttgaacctc
    43861 ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta ttatcttctt
    43921 tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa ttctctacca
    43981 tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc ttagcgctga
    44041 aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct gctacagtaa
    44101 ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat atgtacatct
    44161 gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct ggacgtggtt
    44221 atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt ccagtcgatt
    44281 caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt gttggacacc
    44341 gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt gaaccgttgg
    44401 aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt gtgaacttaa
    44461 ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa aaagaaaaaa
    44521 tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat tgtttgaaac
    44581 gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa atgatcaaag
    44641 tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac gacttaggtt
    44701 taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac gtgagttatc
    44761 gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta acaactgatt
    44821 tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt cgctcaactg
    44881 cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt caagctgcgg
    44941 ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc gtcattgaat
    45001 cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt caaggcggat
    45061 acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg gttgtcttag
    45121 aacttgtcta aaatcacact aataacatca ctttatagat gattctttta gagtgttatg
    45181 atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga atatcgcttg
    45241 gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt catgacagat
    45301 atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt tgtctttttt
    45361 tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc aattattgaa
    45421 ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt gaaagatgtc
    45481 tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg ttcaggaaaa
    45541 tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat cgtgaaagtg
    45601 ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt tggaatggtt
    45661 tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt tgcttttggt
    45721 cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga tgcacttgag
    45781 aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg agggcaaaaa
    45841 caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat tttagatgaa
    45901 gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat caagaagatc
    45961 aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga agcagctaat
    46021 gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac accaaaagaa
    46081 attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt tcctgaaaaa
    46141 ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat gacagaagaa
    46201 aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact tatcagccga
    46261 atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa gaaaacagtt
    46321 atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag catttgaatg
    46381 cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt cagccggata
    46441 cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt cagtttccag
    46501 aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct aaaaactttg
    46561 gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa gttgggctgg
    46621 atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg cgtagagtag
    46681 caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa ccaacggcag
    46741 gacttgatcc ccagggtaga aaagaaatga tggagatgtt ttggcgtctt cataaggaac
    46801 agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat tttgcagatt
    46861 atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa gaggtctttc
    46921 aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc gagtttgcag
    46981 aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct gaagaattag
    47041 cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt gatatttggt
    47101 cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa attattagcc
    47161 agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta ccttttgttg
    47221 gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt ttttatccga
    47281 ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt cttgtttaca
    47341 agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt tggtgtgatg
    47401 aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac tctactcacg
    47461 cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg tccattgaaa
    47521 gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct tcgttttgtg
    47581 cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg cggtgttgat
    47641 tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct gattccttta
    47701 tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc aagaggctac
    47761 aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag agacacgatg
    47821 gttatcattg cttttgtatt attaactgtg ctactgatct ttttgagagg ataaacgttc
    47881 agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat gcgagcagtg
    47941 tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt tcttttatct
    48001 gaaaagaaag aacgaaaaaa ttttataaaa aaatcattat tccagataac cttttaggaa
    48061 caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcatacgacg gaacaaattt
    48121 taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag aaaaaacatt
    48181 acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga cagatgccgg
    48241 agtacatgcg attgggcaag taatccattt tgattatcca gaggagcgtc cgttagaacg
    48301 aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac aagtagaaat
    48361 tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc aatttcgagt
    48421 ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt ttccttaccc
    48481 tcttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc acgatttcac
    48541 ctctttttgt gcatcaggaa gctcgataga ggataaagta cgtacgatct acgaggctaa
    48601 gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa atggcttctt
    48661 gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg gccgattgcc
    48721 tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag gacctacagc
    48781 acatcctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggttg tgacagaagt
    48841 ggtcagcttc aagaaataag aaggaattta cgaaaattac ttctcaaaat tttgcaaaat
    48901 ttcggcttat tttcgaagga gctacttctg gaacaccgtg aatcagaggt tgtgaaaaag
    48961 ctgccctgca tcaagtaata agaacaacca aacaaaaata gctcgtcata tttttcgttt
    49021 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    49081 atcagcgttt tgacctgagt attagagaaa caaacggaaa aatagttcct catattttgc
    49141 cgtttgttga gctaataccg aaggtcagct gattgaacac cgtttaagaa agaggtcgtg
    49201 agcctgtcat ttaactctga gtcacaagta acaatttcaa aaaacagctt ctcatctttg
    49261 atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt gttacctatt
    49321 ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg cccttaatga ggacagtcgg
    49381 aacaggtttg tcatttttat atttaaatgg cacaaatttt tctaattatt tgtcatttag
    49441 cgctgaaaat ttttgctatt tatctaaaaa agtgataact acttaagatg aagcagaata
    49501 tttacgccat acggcataca accgcgaaaa atataaaaaa aagaaaacga ttgaacgcgt
    49561 ttttgcagtc gcaaaggaaa agcatggaat gcgctacatc aagtatcgag acttggaaaa
    49621 caatcaggtg tatacgctga ttgtttttgc tgcaatgaat ctgaaaaagt tgtccacttg
    49681 gcagttggaa catatgtctt catatctttt attatacaaa aatagcaaaa gcaccagtct
    49741 acactcttag taaagaggaa actgatcctt ttgtctacat tcttaaagta cttaaactaa
    49801 gtactttatt ttactcttta ggattgatac agtagcatct atccctttat taataatctt
    49861 ttattttttt aaataaagat gttgtctcca tcttcaggca ctaaaacttt ttcggaaatt
    49921 cctttttctt tattgaaaga ctttaattct tctcttgaca gtccccaatg gtttacagct
    49981 tccatatgaa cggcaataat tgtggaatct ggtattgctt tatgcaattc atataaatcg
    50041 tctttcccca taataagaga ttctccaact aaaaaatgat tatctccagc atttgctact
    50101 atgatttctg gattatactg atcaatagtt tgtttaacag cttcatacca aactgtatct
    50161 cctgtaaagt atagtgtctt ctcagtcgaa tgtttgaaaa caataccgca aacattacct
    50221 acataattca agatttctcc tctgccatgt ttgccttttg ttttaaccaa ttgtatagat
    50281 tcaaaaactg tgtcttctcg tagtacctcc acatttttaa aaccatatag tcgaatttca
    50341 ttcgcatcat tttcatcttg cacaaataat tttatttctt taggtaggac ctctttcgct
    50401 atatcgtccc aatgatctag atgtaaatgt gttacaataa cagcatcaat accgttgata
    50461 atatcttcga ctggcattgg caaatcaact agaggattca tttgatcttt tcttggtgat
    50521 ggaaaaggac ctttcgaaat aaatggtgga agtgttcctt tttccgctaa cattggatca
    50581 attaaaaatt ttttacctcc atagtctaag atagctgttg cattacgaat gtgatgtata
    50641 ttcataaacg ttgtctcctt tttgttgata tgaaatattt ttagtataat tcaattccat
    50701 acaataaata aaccgaacaa aaaaagtctt ttactatttt tacttaattt atgaaaggtt
    50761 gtttaccatg ttagaccaaa cagatataaa tatcattgag gaactatcaa aaaatagtcg
    50821 aatcacaatg aaagaacttg gagaaaaagt acatttgact ggacccgctg tctcggcgag
    50881 agtgacaaaa ctagaggaaa gtggagttat tgaaaattat acagtaaaat taaatatgga
    50941 aaagctaggg ttttcgattc acgctttttt aattataatt acg
//
LOCUS       DANKMN010000009        49931 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000009 DANKMN010000000
VERSION     DANKMN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(138..482)
                     /locus_tag="PW224_000548"
     CDS             complement(138..482)
                     /locus_tag="PW224_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL2907263.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(619..1764)
                     /locus_tag="PW224_000549"
     CDS             complement(619..1764)
                     /locus_tag="PW224_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2907264.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(1944..2918)
                     /locus_tag="PW224_000550"
     CDS             complement(1944..2918)
                     /locus_tag="PW224_000550"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2907265.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(2998..3873)
                     /locus_tag="PW224_000551"
     CDS             complement(2998..3873)
                     /locus_tag="PW224_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL2907266.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            4003..5910
                     /locus_tag="PW224_000552"
     CDS             4003..5910
                     /locus_tag="PW224_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL2907267.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(6451..9729)
                     /locus_tag="PW224_000553"
     CDS             complement(6451..9729)
                     /locus_tag="PW224_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL2907268.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(10000..10545)
                     /locus_tag="PW224_000554"
     CDS             complement(10000..10545)
                     /locus_tag="PW224_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2907269.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(10557..11249)
                     /locus_tag="PW224_000555"
     CDS             complement(10557..11249)
                     /locus_tag="PW224_000555"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL2907270.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(11268..11552)
                     /locus_tag="PW224_000556"
     CDS             complement(11268..11552)
                     /locus_tag="PW224_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907271.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(11565..12128)
                     /locus_tag="PW224_000557"
     CDS             complement(11565..12128)
                     /locus_tag="PW224_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2907272.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            12356..13018
                     /locus_tag="PW224_000558"
     CDS             12356..13018
                     /locus_tag="PW224_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL2907273.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            13142..14338
                     /locus_tag="PW224_000559"
     CDS             13142..14338
                     /locus_tag="PW224_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL2907274.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(14439..15815)
                     /locus_tag="PW224_000560"
     CDS             complement(14439..15815)
                     /locus_tag="PW224_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2907275.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(15852..16718)
                     /locus_tag="PW224_000561"
     CDS             complement(15852..16718)
                     /locus_tag="PW224_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL2907276.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(16807..17832)
                     /locus_tag="PW224_000562"
     CDS             complement(16807..17832)
                     /locus_tag="PW224_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2907277.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(17850..18524)
                     /locus_tag="PW224_000563"
     CDS             complement(17850..18524)
                     /locus_tag="PW224_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2907278.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(18708..19601)
                     /locus_tag="PW224_000564"
     CDS             complement(18708..19601)
                     /locus_tag="PW224_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2907279.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(19675..21372)
                     /locus_tag="PW224_000565"
     CDS             complement(19675..21372)
                     /locus_tag="PW224_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2907280.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(21365..22678)
                     /locus_tag="PW224_000566"
     CDS             complement(21365..22678)
                     /locus_tag="PW224_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2907281.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(22678..26715)
                     /locus_tag="PW224_000567"
     CDS             complement(22678..26715)
                     /locus_tag="PW224_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2907282.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(26728..27186)
                     /locus_tag="PW224_000568"
     CDS             complement(26728..27186)
                     /locus_tag="PW224_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2907283.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(27484..28002)
                     /locus_tag="PW224_000569"
     CDS             complement(27484..28002)
                     /locus_tag="PW224_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2907284.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(28004..28621)
                     /locus_tag="PW224_000570"
     CDS             complement(28004..28621)
                     /locus_tag="PW224_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2907285.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(28618..29472)
                     /locus_tag="PW224_000571"
     CDS             complement(28618..29472)
                     /locus_tag="PW224_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2907286.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(29469..30380)
                     /locus_tag="PW224_000572"
     CDS             complement(29469..30380)
                     /locus_tag="PW224_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2907287.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(30421..31755)
                     /locus_tag="PW224_000573"
     CDS             complement(30421..31755)
                     /locus_tag="PW224_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2907288.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            31963..32793
                     /locus_tag="PW224_000574"
     CDS             31963..32793
                     /locus_tag="PW224_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDL2907289.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(33037..33355)
                     /locus_tag="PW224_000575"
                     /pseudo
     CDS             complement(33037..33355)
                     /locus_tag="PW224_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(33477..33671)
                     /locus_tag="PW224_000576"
     CDS             complement(33477..33671)
                     /locus_tag="PW224_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907290.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(34176..34361)
                     /locus_tag="PW224_000577"
     CDS             complement(34176..34361)
                     /locus_tag="PW224_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907291.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(35211..35552)
                     /locus_tag="PW224_000578"
     CDS             complement(35211..35552)
                     /locus_tag="PW224_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2907292.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(35552..35782)
                     /locus_tag="PW224_000579"
     CDS             complement(35552..35782)
                     /locus_tag="PW224_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2907293.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            36069..36407
                     /locus_tag="PW224_000580"
     CDS             36069..36407
                     /locus_tag="PW224_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907294.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(36612..36851)
                     /locus_tag="PW224_000581"
     CDS             complement(36612..36851)
                     /locus_tag="PW224_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2907295.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(36827..37174)
                     /locus_tag="PW224_000582"
     CDS             complement(36827..37174)
                     /locus_tag="PW224_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2907296.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(37906..38487)
                     /locus_tag="PW224_000583"
     CDS             complement(37906..38487)
                     /locus_tag="PW224_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2907297.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(38670..39296)
                     /locus_tag="PW224_000584"
     CDS             complement(38670..39296)
                     /locus_tag="PW224_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2907298.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(39318..40649)
                     /locus_tag="PW224_000585"
     CDS             complement(39318..40649)
                     /locus_tag="PW224_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL2907299.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(40804..41205)
                     /locus_tag="PW224_000586"
     CDS             complement(40804..41205)
                     /locus_tag="PW224_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2907300.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(41291..41497)
                     /locus_tag="PW224_000587"
     CDS             complement(41291..41497)
                     /locus_tag="PW224_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907301.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(41592..43094)
                     /locus_tag="PW224_000588"
     CDS             complement(41592..43094)
                     /locus_tag="PW224_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL2907302.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(43112..43567)
                     /gene="argR"
                     /locus_tag="PW224_000589"
     CDS             complement(43112..43567)
                     /gene="argR"
                     /locus_tag="PW224_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2907303.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(43665..44282)
                     /locus_tag="PW224_000590"
     CDS             complement(43665..44282)
                     /locus_tag="PW224_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL2907304.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            44503..45876
                     /locus_tag="PW224_000591"
     CDS             44503..45876
                     /locus_tag="PW224_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2907305.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            45951..47063
                     /locus_tag="PW224_000592"
     CDS             45951..47063
                     /locus_tag="PW224_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL2907306.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(47135..48772)
                     /locus_tag="PW224_000593"
     CDS             complement(47135..48772)
                     /locus_tag="PW224_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL2907307.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(48940..49548)
                     /locus_tag="PW224_000594"
     CDS             complement(48940..49548)
                     /locus_tag="PW224_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2907308.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        14333 a        10551 c         8740 g        16307 t
ORIGIN      
        1 aaatcagcaa aatattaaaa ataacaacat gtgttaaatt taaaaataaa aaagacaact
       61 aatttgcgtc gtccttacaa agttagtcat ctaacaatag gccgtttcaa aactagttgt
      121 ctttttatat ccacagatca ttcgactttt tcgaataacc cacggctaat catcccatcc
      181 atcagaaaat aaaatttatc ttggctcatt ttctgttttg gatctttgaa aatattgact
      241 gctttcaatc catttgctgt agtgatcgac attttcaatg tttgaatatc ttttgataca
      301 gtgattttta atttgaatcc ttctttgctt tgcggcgtgg catctaatgg tcggatcaat
      361 tgataattgc ctccgtttgt ttctgtaaat ccattatcgc gcaatgtata tctttttgca
      421 tcaggatgca aacgatagct aattggtgcg cccagtaccg ttgctgttgt ttcaaatgcc
      481 atgattttca cctcgataaa attagtttct ctttattata aaagtttatc ctgattttta
      541 ctaggttttt cagttaagtt ccctaaaaaa tgaaggcgtt ctctcagaca tctgttcata
      601 taagaatgac ttcactagtt attttttgac agcacgaact aattgatcaa ggaaatactg
      661 gatgtcgtct tcggtatttc ccaaaccaaa gctgatacgg atggattctt ttagtatcgg
      721 tgtatcttct ccatacattg cttctaatac gtgtgaaggc tcgatatttc ctgctgtaca
      781 agctgaacca gtagaaatag cgaccccatt aagatcaagc ttcatcagta gcaaatcatt
      841 tgcttcgcca attaagcgaa gattcaacac atgagggagt ttattcgtta aatcaccatt
      901 tacttgataa gggatttcgt ttgcttctaa tgtatcaaga atcaattgct ggaatccttt
      961 atatttgttg ttacgtgctt cacgttcttc aggtgacagc agttcaactg cttttgccat
     1021 accccagatc cctgctaaat tttctgtacc tgcacgccgc ttttcttcct gctccccgcc
     1081 taaaagcaat gcagggatat ttactccgtc tcgtttatat aagaaaccga cgccttttgg
     1141 cccattgatt ttatgagcag agatacttaa taaatcgatt cctaattctt ctggatggat
     1201 cgtttggttt ccgtacgctt gtaccgcatc tgtatggaaa taagctggat gatccgctaa
     1261 aagttcaccg atttctttga ttggcatcaa atttccgatt tcattgttcc cgtacatcac
     1321 agagaccaag atcgtatctt ctcgtaacgc ttttttgacc tcttcgatcg acagattccc
     1381 tttttcatct acaggcagat atgtaacgtc aaaccccttt gtctctaaat atttcatggt
     1441 attaagaaca gcaggatgtt cgatagctgt agtgattaga tgtctgcctt ctttttgacg
     1501 cgagaacgct gtttctaaga tagctgtatt atccccttct gttcccccac tgttaaagat
     1561 gatctcgtga ggttttgctt gcagactgtc tgcaatgatt tgtcttgctg tttccaattt
     1621 ttcatgcgcg tggcgaccaa atccatgtat gcttgaagga tttccaaacg tgtcttgcat
     1681 gattgccgtc atcgcttcaa ttacttgtgg atgcatgggg gtagttgccg catgatccag
     1741 ataaatagtt ttcacttcat tcatcccttc caaattattg ctaggcacat tctagcataa
     1801 aatagcgaac aaaccaaaga agagctcatt tttgtctaga ggacgtttca tgggcataga
     1861 aaaaaggagc tgtgacaaaa gtcttgtcgc agctccttga agctgctcac ttctgtcaca
     1921 acttctttcc ctcttttttt tgtttattcg aattcttttt cttccagacg gaataacgga
     1981 ctcatcggtg tattttcatg gatgcgtttg accgcttcac ccattaattc tgaacatgta
     2041 acgattgtta agttttctgg atgacgattt tcttctgtga aaacagaatc agtgatacaa
     2101 atatctttga ttggtgcatt gtctaaaata cttttcgcat gatcggacaa taatccatgt
     2161 gacgcacagg catatacttc ttttgcgccg ttttctttta atacatttgc tgctcgtgca
     2221 agcgttgctc ctgtgttcaa gatatcatct accatgatac atgtctttcc ttgaacatca
     2281 ccgatcacat aaccaccctc agttgaagca tcatctgcat gatctacgat ggctaatgtg
     2341 ctgttcaaat attctgaaag actacgtgca cgctgaacac cgctgttttt tggcgaaaca
     2401 actacgatgt catctcccgt cagacctaat tgacggtaat aatgagcaaa caacggcatc
     2461 gtaaataaat tatctacagg aatatcaaag aatccttgta cttgaaccgt gtgcagatct
     2521 aaagtcagta ctcgagttgc ccctgcttct tcgatcaagt tagcgatcaa tttcgcagtg
     2581 atcggttcat gaggtttcgc cgtgcggtct tgcctagcat acccatagta aggcaatacg
     2641 acattgatcg ttttcgcact tgctcgcttc attgcatcga tcatgatcag tagttccata
     2701 tagtaatcat tgacaggtgc attcgttgat tgaacgatat acacatgatc ccctcgaata
     2761 ctttcttcga tattgatgga aatctcgcca tcactaaatt gcttcacgac gcttttacct
     2821 aattctgttc cgaacacttt cgcgattttt tcagctaatg gacggttgcc attcaaacta
     2881 aaaatcttca acgtgtcatc ctgatactta ttactcatgt agtcctcccc aaaacttctc
     2941 tattcttcga tagtttaaca tatttttcca agattttctg ttaatttcag caaattctta
     3001 aggaagtgaa actgttccgt tactccaact ggcaggaatg actttatgat aatcgcccca
     3061 gttgtcgtgt actttgtaat aacgttctcc tgtttcagtt tcaaaataag cgtacgctgt
     3121 cacccaatga ttgccatatg tactgccaag tactttcaaa ataccaatca tcacaggttt
     3181 gccttcacgt atacgttttg tagctcgctg ccatgaacct agaagagttg ctcgtgcacg
     3241 ctcatgattg ccattcttct ttaaaaaact agaaatccct gtactaacct gaaaaggaat
     3301 cgtaggaagt cctagtggtt ggatctggct acgcaatgcc ttcgtcaaaa ccaacgaatc
     3361 agccgtattt ttcttgcgaa tctcatttgg aagcatccac cccttacgat agtcttgata
     3421 ataggcaagg agaactgctg ccgcgtaagt accacaaaga tagcctgagg gggtagccca
     3481 tttcttatac ctcaaaaaac gttcttctgg caatcccacc cagcaatttg cgagttcctt
     3541 tcctgacctt ttttcttcga tcaaaacggg aatatcttct tttctgttga tccatctaga
     3601 taactcgatt ggctcttcat tgatcagctt ccatttttta caagaaagac cctcttgata
     3661 agtatatttt gctggagaat attctaccgg atgtcctgat tgatcgataa tcacataact
     3721 ttgatcttca aaaaccagag ctaaaaaaag caatttactt tttcttgact gcagctgctg
     3781 agtgctggct aattttttaa tttttttatc tactttgaaa tataaaggtt tatccactac
     3841 ttgattcagt tgggatcgcc tccttttttt cattataagt gaaggattga aaaaaacaaa
     3901 tcagtttgat tgttcgaatt ttcaattttt tatgttctat ttgttaattc cttcattttg
     3961 taagataatg ggcacacgaa acaaaaaagg agcgatcgat gaatgaacaa agaacgattg
     4021 aaacaagatt tctatgaagc agtcaatgaa gaatggcaaa aaacagcaaa aatccctgca
     4081 gataaaccag ctacaggcgg tttccaagat ttagttgacg ggatcgacca acttttgatg
     4141 ggagaaacag acgatctgct tgcccatcca gaaaaagtgt cttcagaaaa aatgaaacat
     4201 ttccttgcat attatcaact ggcaaacgat tatgaccgaa gaaatgagtt gcatgctcaa
     4261 ccgcttcaac ctataatcga aaaagttgaa tcattgcagt ctttcgacga attgaataag
     4321 caatttccag aatggctgtt ggatacatta ccattgccat ttaatctgga tgtagatgca
     4381 gatatgaaga atgcgcaaac gaacgccttc tttgcttatc cgccttcttt attccttcca
     4441 gataagacgt attatgcatc agaccatccc aacggtgcac aactaatagg cgttttcttt
     4501 gatatggctg ttcagttatt agagcttgcc ggcaaagaaa aaagtcaggc agaagcaatc
     4561 acggaacaag cagtgcaatt tgataaattg attgctcctc atgtaaaaag tgcagaagaa
     4621 aacgcagatt acagcaaaat gtataatccg caatcttttg atacatttat taaatacaca
     4681 gataaactgg acttgaaaaa acttgtcacg ggtttgattg ggacaactcc agataaagtg
     4741 atcgtaactg atccagtgta cttcgatcat ttgaatgaca tcctgacaga agctcatttt
     4801 ccgttattca aaagctggat gatcattaaa acagtacgtt ctcttagtgg ttatctatca
     4861 gaagaattcc gccaagtcag cggaatctat tctcgcgtat tgtctggaac agacgaagca
     4921 atgcctcaga aaaaagcagc gtattacctt gcctctggac aattcgatca agtagttggc
     4981 gattactacg gaaaaaaata ttttggcgaa gctgctaaaa aagacgtaga acaaatggtg
     5041 aaaaaaatga tcgccgttta taaaaaacgt ctagaagaaa atgactggct tggggatgca
     5101 acaaaagaaa aagccgtcat aaaattaaat aacttaggta tccaagtagg ctaccccgat
     5161 aagatcccag cattattcga ccagttcaaa acagtacctg cagatcaggg tggtactttg
     5221 ctttcaaata ctcttgtttt cagcaaactc acattaaaag atcgtttcag caaatggaac
     5281 aaaccagtgg atcgttcaga atgggaaatg agtgctgaca cggtcaatgc ttattatcat
     5341 ccatttcgga atgtgatcgt ctttccggca gcgatccttc aagcaccatt ttacagtcta
     5401 gaacaatcaa gctctgctaa ttatggtgga atcggagctg ttatcgctca tgagatttcc
     5461 catgcatttg ataataacgg cgctttattt gacgaatatg gcaacttgaa caactggtgg
     5521 acagaagaag atcttacaca atttcaaaac aaagcacaag caatgatcga agaattcgat
     5581 ggtctgcctt ttgctgacgg aaaagtaaac ggaaaattaa ccgtttcgga aaatattgct
     5641 gatgcaggcg gtctaagctg tgcattggaa gcagcaaaaa cggaatcaga tctttctatc
     5701 gaggaattct ttgtcaattg ggcaacgatt tggcgtacaa aagcaaaaaa agaataccaa
     5761 caacttttac tgcaaatcga tgtccatgcc cctgctaaac tccgagcaaa cgtgcaacca
     5821 aaaaaccttg acgaattcta tgaaacattt ggtatcacgg aacaagacgc tatgtatcta
     5881 gcgccagaaa aaagagtgcg aatctggtaa gaggttgtgc cagaagtggt cagcttcaag
     5941 aaataagaag gaatttggga aaatcgcttc tcaaattttt gtgacatttc ggcttatttt
     6001 tcgaagaagt tacttctgga ataccatgta ttagaggttg tgaaaaagct gcttagtttc
     6061 aagtagtaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttac
     6121 taccgagaaa ctggcttttg aacaccgttt atcgagaggt tgtgaaatca gcgtcctgac
     6181 ctgagtatta gagaaaaaaa cggaaaaata gttcctcata ttttgccgtt tgttgagcta
     6241 ataccgaggg tcagctgatt gaacaccgtt tagtggagag attgtgaaaa agctgtcctg
     6301 catcaagtaa taagaacagc caatcaaaag cagcaaggag aacctcatct ataaaaagat
     6361 agaaggaagt gagacatcta ttggtctcat ttccttctat ttttactttc ctcagtcaat
     6421 tgttttacat cataaaagga gaaattccac ttattttttt cgctgtttca tgacataaac
     6481 aactacaccg attaacaaaa ttcctccagc taatgctagc aacgggttat taaattctcc
     6541 cgtttttggg agtccttgag tgccattttt ttcactttct tttgctgtcc tatccggatg
     6601 agaaggtttt ccatttggtt ttccatttgg tgattcattt cctggatttt ctctttctgg
     6661 tttgtcattt cccgggggca catcggcagt ttctcgcaca gtgatcgctt tttgctgggt
     6721 aacagattct gtatgagcag accatttttc cccatcaaat atttccgaac gataagtgac
     6781 ttctagcgta tattctcctt ttgaaggtgc tgaaaaaata gcggtatgat tagaaaattg
     6841 agcacttgac tgtgtttcaa cgatttggat cttttctggg aaataacgca tatcttcaaa
     6901 gacaggcgtt ccttgatttg gtgtgaaagt tgacacaaaa actgtaaaag gcttcagttc
     6961 aatgatattt ccttctgcat tctccgccac accatttgct tcttgtataa taatcggatt
     7021 attttgttca tttggttttg tctgatttgg ttcttgtacg attgcatact ctggatattc
     7081 cgaagacggt gaggtttctc cgtcaaaaat agcacctaca gataatggaa gaccatccac
     7141 tctttccttt tctgtttgac gattccccgg tttaatctgc atcagtccat gaacatggat
     7201 acttccattt tcatagcgcg taacaggctg ctgaccttct ttcgaacatg tatttagaga
     7261 ctcgatccaa tcttttgata tggtatgtcc aagagtattt tctgattgtt tctctcgttc
     7321 attccaatag tagttcaacg agtggtagat ttgatcttcg ttttgattca atgggcggat
     7381 ctgctctctc atctcaagat tctctgttcg gaaagataag ataccagaag catggtagtc
     7441 tgtgtttcct gctgaagagg tataaagagc cacattatat gaaccgttat taaaacttgt
     7501 actgctattg acgataatat ctgttccgct attggaatcg attcctttcg caagattttc
     7561 gaaagacaaa ctattgatca attcatgagg gcccgttaaa gaagaaccgc ccattttgaa
     7621 tccattaccg ttaccttctc cctcaattcc tggtacccag ccgtttcgat aagcaactga
     7681 gtttttgatg attacttttc cgatcgaacc tgtttcattt ttagcaaaaa ggtcccaacc
     7741 gtcatctgca ttatgatacg cgatacaacc gtcaaataca tttccttctc ctattgtcag
     7801 tttggccgca aatccatcgg catcttcaaa tcctggatcg gcattttcaa aagatgtaca
     7861 gttcttgact aagttatgcg cgggccatgc ttcgaaagga tcatttgagc taccgctgat
     7921 ttggattcct gtatttccat tccgataagc atgaatctct tcgatcaaat tatgatttcc
     7981 agaaatctgt aatccttttt gcatagctgc actatttgta atgtcaaacc ctcgtaaacc
     8041 ccagtagtga ctgaacagtg tcatcccatt tccagttcct ttaaaatcaa gtacaggacg
     8101 cttgttttct ggatttttct ctgcaatcaa ctgaatcggg ttttctcttg tcccgttaac
     8161 gccttttgga atcgtcaatc ctctttcgaa ggaataagtt ccaggcgcca tgacgattgt
     8221 ctgcccagca tatgcgtatc tcaatgcttg tgtcagagat aatggatttt gtctgcttcc
     8281 gtttcctgca tctgaccctt ctggtgttac ataaactgtc gaatatttag gtttacggta
     8341 gtccactgta tgaagaatct cttttgtttc ataactagtc atctctacgt attcttctgg
     8401 cggatagttt ttatctggcg taaaactgaa agtaaattcg ttcgttcctt ccatcagttt
     8461 tgtctgataa tcaaattccc gattcgcagt gatagccaag ttttttgcgt tcttaagcac
     8521 ctctcctgtt gctttatttt taattgtcaa taatccgtca gcatttgctc taaaggacat
     8581 agtatgatct tggtttcctg aatgattact agaggtcaca gtgtaaatcg gatcaatcaa
     8641 gcgagtaggt ctttcttgcc caggaggatc tgtcgcagga tctgagtaag caacttcgat
     8701 tccttctacc ttgatcgtca tattccttgc tgcagcaaat cctgcataaa taaccgattc
     8761 atctgaagcg tataattttt cccagtcata catgatgtca gaagccattt cttctcctgt
     8821 ttcaggatcg taataatatg cctctaaacc agtattcgtt tttttcatct ctacgtccag
     8881 tttagtaatc gagttcgtgg ccggaaacag attcccatga ccgtttccga atatattata
     8941 agaacctgtt tctttttcca agaaccctgc tgaagtctct aaaggtgttg tttccactga
     9001 gacactccca ggagctggcg cagcttgcgg atcttcagat gatattcctg tgattgcccg
     9061 tgtccccaat ccaagtcgca tcgtatattt gctgccgctc gtgttgacca cttcttgtct
     9121 atccgagtcc cagacatatt cgatccgact gcctagtatg gcaaatgaat ttgaataaaa
     9181 tgcctgccca aacaaatgat tttttggtac tgcgtctctt accattaaag caaatccttc
     9241 ttgggcattc gtataggtcc aagattctat ttcgatcgtc gcccgcatcc taaagttgat
     9301 gtctttgggg attgcttgat aataataggt cattccatct gatccgcttg actggaattt
     9361 tccgccgtta ttcgtggaag agagccgcac gccgtcttct aatggttcaa tggcatacct
     9421 actagcactt gtgctttctc cgaaaacaac ccctttccac tcttcatttg cttcttcaga
     9481 ttgctttttt gtcttttcat tttctgattc tttcatcata ggatcatttc cttcttccgt
     9541 atccatatct tcactagttc ttggtatttc ctccattgtt gacatccctt caatgttcac
     9601 gtgtgcattt tcttcgttca cagactgttg ttcgttagca gcaacttcaa cgacagaaat
     9661 taaaaccggg gtactgagca taaggagatt catcattagc gcactgtatt ttatacgttt
     9721 ttttaacaag tgtattcctc ccgtaacaaa ttagaaagcg tttacttaaa gtattaccta
     9781 atttattttt actgtcaata aaaaacaaaa ttataataag tgtattttat aaaaagaaaa
     9841 aatataactt aagttgttga taataaagag atttattaac gtaaaaagaa taagtttttt
     9901 taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct cttcttttat
     9961 tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag
    10021 tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacgga
    10081 atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac
    10141 acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg
    10201 gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt
    10261 ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc
    10321 cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg
    10381 atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg
    10441 agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc
    10501 tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat
    10561 acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata
    10621 aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc
    10681 aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa
    10741 gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat
    10801 gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta
    10861 accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt
    10921 cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata
    10981 acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc
    11041 atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct
    11101 ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg
    11161 cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct
    11221 tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg
    11281 attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa
    11341 gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc
    11401 tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt
    11461 tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt
    11521 tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc
    11581 aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc
    11641 atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct
    11701 tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca
    11761 tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag
    11821 ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga
    11881 tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg
    11941 acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat
    12001 accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca
    12061 ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg
    12121 ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat
    12181 ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc tctaaatata
    12241 gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa taagtatgta
    12301 atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat
    12361 aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa
    12421 tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact
    12481 ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact
    12541 tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac
    12601 aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa
    12661 acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga
    12721 attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc
    12781 gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa
    12841 acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat
    12901 cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc
    12961 tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact
    13021 cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt
    13081 tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt
    13141 tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga
    13201 tttattgaac aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga
    13261 gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga
    13321 acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt
    13381 catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa
    13441 tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg
    13501 tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt
    13561 cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc
    13621 acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa
    13681 tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta
    13741 tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat
    13801 gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca
    13861 attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat
    13921 ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat
    13981 ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac
    14041 cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga
    14101 cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa
    14161 tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac
    14221 aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt
    14281 agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat
    14341 cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg
    14401 cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt catcttgctg
    14461 tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg
    14521 atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaaaat ggagacgatt
    14581 ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt
    14641 ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt
    14701 agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg
    14761 gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga
    14821 agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc
    14881 gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt
    14941 cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg
    15001 attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca
    15061 atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg
    15121 gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa
    15181 ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata
    15241 aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc
    15301 ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca
    15361 atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct
    15421 aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct
    15481 agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca
    15541 acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa
    15601 cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga
    15661 ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca
    15721 ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga
    15781 ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg
    15841 gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa
    15901 aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa
    15961 atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa
    16021 ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa taatccacag
    16081 atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg
    16141 cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag
    16201 ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc
    16261 ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca
    16321 gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc
    16381 gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa
    16441 ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca
    16501 ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg
    16561 attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt
    16621 cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc
    16681 catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac
    16741 atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac
    16801 gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt
    16861 cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt
    16921 tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt
    16981 aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt
    17041 aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc
    17101 cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc
    17161 atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa
    17221 ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat
    17281 gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc
    17341 atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc
    17401 gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga
    17461 gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg
    17521 ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc
    17581 actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct
    17641 tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc
    17701 taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat
    17761 cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt
    17821 aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt
    17881 gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg
    17941 actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt
    18001 tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt
    18061 ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta
    18121 atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt
    18181 aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct
    18241 gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg
    18301 atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga
    18361 agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga
    18421 ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat
    18481 tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc
    18541 tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa
    18601 aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct
    18661 cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt
    18721 ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc
    18781 actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt
    18841 tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg
    18901 tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta
    18961 attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg
    19021 agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc
    19081 gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct
    19141 cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca
    19201 ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc
    19261 atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa
    19321 ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc
    19381 ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt
    19441 tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga
    19501 tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga
    19561 ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt
    19621 aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac
    19681 ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc
    19741 gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat
    19801 attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata
    19861 gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc
    19921 ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa
    19981 gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct
    20041 atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc
    20101 agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc
    20161 atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc
    20221 ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc
    20281 atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg
    20341 tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat
    20401 cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat
    20461 cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt
    20521 atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa
    20581 ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata
    20641 agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc
    20701 ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc
    20761 tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat
    20821 catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata
    20881 agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac
    20941 ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg
    21001 atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga
    21061 attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac
    21121 ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt
    21181 agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa
    21241 tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt
    21301 cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat
    21361 ttttttattc atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata
    21421 aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc
    21481 attttcctga ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt
    21541 cttttacggt ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt
    21601 tcccggattc taattcagcc atctcctgtc cgttaactac tacatattgt ggttggcctt
    21661 ttgcatccac ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct
    21721 cgtaaccttc cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt
    21781 gtcctgtcgt aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca
    21841 aaggatcatt gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat
    21901 ctaaataata tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg
    21961 gttttccttt ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta
    22021 gataaccgac atttttagga tagatatgaa tctcttgcag ttcctcggtt gattctgtcg
    22081 ggtgataaat tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca
    22141 atccaacttc tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt
    22201 ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc
    22261 cagttgttac cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat
    22321 tgtttctgtt cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag
    22381 cttttttttc atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg
    22441 gcaccgtctg tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat
    22501 cattttcatg gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca
    22561 acacgaattc cacgacatct tcccctgatt cttctgccga catcgcatgt ggaagaagga
    22621 ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta
    22681 ccttgctccc tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc
    22741 catgaagaat gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa
    22801 cggattttca ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag
    22861 ttcatattct aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga
    22921 aatctcaact tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc
    22981 aggttcttct cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc
    23041 aggtgacttc acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag
    23101 ggaagaatcg tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact
    23161 attttgtttc tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat
    23221 tttcaaaagt tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc
    23281 atcgatgatt tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc
    23341 aatgataaac ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata
    23401 cccatttggt gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg
    23461 gtcgtaattt aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat
    23521 cccaggcaaa cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag
    23581 cgggaaggaa gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc
    23641 ttcatggtct aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa
    23701 tgctacccat aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc
    23761 acctgtttgc ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt
    23821 gtcattttca tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc
    23881 tgcatagttg gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg
    23941 atgaataact ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa
    24001 cgtgagtctc cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg
    24061 atatccgatt tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat
    24121 tggcatatta ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt
    24181 tcgcatttcc agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg
    24241 tacaacagaa agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg
    24301 tccgccgact ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc
    24361 atcttcgttg gctgcatatt ttttgatttg gaattgatac gtctcatcta ctaactgata
    24421 acgtggtggt ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc
    24481 tcctaaaatc ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt
    24541 ttcgattggt tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc
    24601 agccaacggc tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc
    24661 gtaacgaatg gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc
    24721 ctccaggttc tgaatatagg ccatattgtt cactgtgcaa aaatttccgt cttgagctcc
    24781 cgcatcaagt tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg
    24841 agaaaattta taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc
    24901 tggtggggct tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc
    24961 gttttctcct ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg
    25021 tgagacatta aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt
    25081 agaagagata tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg
    25141 cggtgttacg ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc
    25201 atctagtcgt tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact
    25261 tggcgggttc tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc
    25321 agatgacttt tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc
    25381 aacccagtga gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt
    25441 tacttcatct tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc
    25501 attagaagta ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc
    25561 aatcttatct tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact
    25621 tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt
    25681 ttctggattt ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag
    25741 ccctagtgta agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc
    25801 cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt
    25861 attgatagct gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg
    25921 gtcagagagc agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt
    25981 ttgactattg gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac
    26041 gatatcgatt ggactgattt cctttttctc gcccccgatg acatctagat agacattgta
    26101 aagggttgtt gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc
    26161 tacccatttt ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc
    26221 atttccccaa agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga
    26281 tccattatta gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact
    26341 atcatcgatc aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt
    26401 gctttgtgtc caatttccta acacttttgt atagtcattc gttgtaaact tcgggtagcg
    26461 atgctgttcg ttttcaactt ctccgctagg atagatatat tcataggggt cttgcgtagc
    26521 tgttggccca agacgctcca aaataccgtt gaataactgg cgttgaatgc gctggagtgc
    26581 ttgaccgctg ggtacgattg gttcgtccag cttttccatc tcattttctt cgctacgttg
    26641 aatttcagcg gaatcttctg tatcagtttc attttcttgt aattcttggt tatctattct
    26701 ttcatcaacc accacttgaa gaggtagtta cccttcgttc acatcgggga tcttttctgt
    26761 ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa acttttccga cataccattc
    26821 ttgaccatcg atcgtctcga cttttaggtc ttctccatct tcatcatagg aggatagacc
    26881 tgttccacga acgtttttta cggtaccaat cccggcggct gccgcatcga ttgctaaacg
    26941 tattctacct tcattggcac tttccttttt ttcaaaagcc agatgccatt caagggtttc
    27001 ttctttttca atatacttcc cttcaatttt tgcatagtcc tcatctaaga atacctcatc
    27061 ctcctttgtt tctgttgact cagcagctac atccagggtc gtcatatgat agactgactg
    27121 gaaatatggt ccaaatagca aactgacagt tactaactgc aaaagcttca attttaaccc
    27181 tttcacatcc ttatactcct ttcaaaaata tttgtcttct ttcacaacaa tcaacaaaac
    27241 aatgcccatc taattataat ttaatttaat tttaatttta aatcaaatga tatccttttt
    27301 agttaaaaaa caccaattat acaacattta ttattaaaat taaattataa aattttataa
    27361 cgttctcatt taacaaaact taagttatta agacaaatga ataaacaact acgctttcct
    27421 cgaaacaaaa aaataaagca caaatgcgca tttacgcact catgctttat tcgtacaatt
    27481 aattcagata acggcagcgc ataggatgcc aataacactt cctaaaatag ctcctcctaa
    27541 aatgtcagta ggataatgta aaagtaaaaa aatcctcgaa taagcgatca aacaagcaaa
    27601 taaaaaggct aatattcctg caactggact taattcgaac aacgcagcta ctgataaaaa
    27661 ggctagcgct gtatgtcctg aaggaaaaga agaagtcttc ggtttacttg tgtaaaggac
    27721 aagccgacga tttttttcat aaggtctttt ccgtcttgtc gtttttttga taaaaagcaa
    27781 accaacaaaa aaatagatca aactgccgat aagaatggct agaccatatt ctctagtatc
    27841 aggtatcaag actaaaacag cagcaaggag caaccaaata aaaccaccat tagagatatt
    27901 actgataaat ttcaaaaaat tgatttttcg actatccgtt ttccgttgct tggcgaactt
    27961 gttgataata ctatcatcga ttttagcaat caaacgcaac atgttattcc tcctttttaa
    28021 gaaccttttc ttgcgtacga tggataaaaa gcaggacttg aaaataaaac aagaaactga
    28081 aacctaataa agcaaaatag attggtcctg caaaccagaa tgcgacaaga aggatcaacg
    28141 tgcctgccaa atagaaaagc gcgcttttga aatcattaaa aaacaagaaa aatgcagtaa
    28201 tccaagaagc ttctttttgt tccagttgaa gcaagatttt gtaactaaag agaagcgcgc
    28261 ttaatagaag caaaaacaac gtagtataca tcaatgtata gccaattccc ccaccccaat
    28321 agcggaaaat ctgataatcg atcaatagaa gaagaaaata aacacttgct aaactatctt
    28381 gctttccata aagagagaag ttctcacgaa actttttcca gaatagtgaa ccgagctttt
    28441 tctcttcatt ttttttgtaa tgataaatca tatacagaac tgttcgactt gcagttccta
    28501 atgtgaaaac aggcaagacg cacaggaacc atagtagctg taaatggata agacgtggaa
    28561 tccattcaat gatttctaag aaataatcat tggctttttt accaatcgat cgattcatca
    28621 tccttttgaa cctcctgtca aatccatccc ttcaatgaaa taacgttgtg caaaaaagaa
    28681 cagcaaaatc gtcgggatca agacaagtgt tgctcctgcc atcagataat tccattgtgt
    28741 cgttgcttgg ttctgaaaaa cttgcaagcc aatctgtaac gtgtattttt cttgatcttg
    28801 gatatataac agaggaccta agaaatcatt ccaagctgcg ttgaaggaat tgatcgcaat
    28861 ggtaatcaag gcaggtttcg tcaatggaag catcaaatga ctccaaatat acaaatgatt
    28921 cgctccgtcg atttcagcag cttcgatcaa ttcattattg atcgataagt aaaattgacg
    28981 catcagaaat atattgaacg cattgccgaa gaacgatgga acgatcaggg gcaaataagt
    29041 acctacccag ccgatcttgg agaataatac atattgaggg atcatcgtta caaaccccgg
    29101 gatcatcatc gtagaaagaa ccaaagcaaa catcagcttt ttcccaggaa aatcaagctt
    29161 cgcaaatcca taagcaataa aagaatttga aaggacatta ccgatcacta ctagcacagt
    29221 gatgaataat gtatttcttg catagcgcca aaaaggaaag gcggcaatcg tatccagata
    29281 gttttcccat acgatttttt ctgggaaaaa ggtcggagga taacgaacga tttcttccat
    29341 tggtttcaat gaagtaaaca ccatccaaag caatggaatg atcaaaatga ttcctattac
    29401 tgtcatgagg acataactta gtattttttt tgtttgccga ttcacactgg tctttttgaa
    29461 ataatccatc atttgccgtc acctccagaa taatagaccc acttaggtgc aagcttcaaa
    29521 ttgacaaacg tcagaaccaa cacgatcaga aacaggatca ttgacatagc catcgcataa
    29581 cccatatcaa aagctacgaa cgccttgttc cacatatgca gattataaaa aagcaaagaa
    29641 tttgcaggtc cgcctgttcc atttttcgtc atgacataag cttcttgaaa aatttggaaa
    29701 gaaccgatcg tagaggtgat cacatcgtaa aaaataatag gagtgattaa tggcaaagta
    29761 atacttttga actttcccca aacgcctgct ccatcgattt ctgctgattc atataaagat
    29821 ctaggaacat tttgtaatgt agctaagtaa agtagcattg ctcctccaga actccatatt
    29881 ttcatcaaaa tcaacgcagg tttcgtccat tgtggatcaa acagccaagc tggtccaggg
    29941 atatggaacc agcccagaaa tgtgttgatc aatcctgcag atggagaaag cagctgcatc
    30001 cataagatgt agactgctac tcctgataag actgccggta aatagaaagc tgtacggaat
    30061 acactgattc ctttgatttt ttgattgagt actgtggcta aaaacacact gaaaatcgtc
    30121 gtcagcggta cattgaagat gacgaaataa acggtgttgt ataacgattt ccaaaataaa
    30181 tcatcgtgaa cgaacattct tttgaaattt gctagaccaa tgaaattcat cttggatgtc
    30241 aaattataat cggtgaaact accaataact gaaaaaagca taggccctag cataaatacc
    30301 aagaacccga taataaaagg cgagataaat aaataggccc atcttgcttc ttttttcttt
    30361 cggatagata aacgcttcaa attttcttgc ctcctctgta cctagacagt caaaaatgga
    30421 ttattgattt tgcttgacta agttttctac tgctttttgt gcatctgcca atccttcttt
    30481 tggtgatttt gttcctaaaa gaatctggtc aatctgttca ttcactaatg tcgtgtaatc
    30541 cggagcatct aaaggaactg gtgtgatcac cgtattttta aagttttcgt ccgccatttg
    30601 atagatcatt tttcctttat ccgttaggtt gtcattattc actaaattct cattggctgt
    30661 cttgctcgcc atgatatcaa aacttttttc gccgaatttt tcttgtactt ctggagaaga
    30721 aagatatttg atgaattcat aagaggcctc aggatctttt gctccgtatg gtacttccaa
    30781 gacgaatccg ccaccccacg accaatggcc tgaaccttct tctttttctg ggagctgcat
    30841 cacgccaaaa tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat aattgatatt
    30901 ttgggcacgc atggctacta atccggaaat aaatggatca gatacaccag acccaaattc
    30961 ggcttctaat ttattgatcg tatttttccc ataataatct tgccagtctt ggatccactc
    31021 caaggcttcc actttatttt tagtatcaat cttgactttt tcatctttgt caaaccaact
    31081 agtcccgtta tctgcgttta gcgcccatac atcagcccct aaattccata atggataaaa
    31141 accgatgcgt gtaaattctt ttccttcttt tttatctagt ttacgtgctg tttcttctaa
    31201 ttcatcccaa gttgtcggta gatcttgttc actgattcca gcttctttaa ataacttttt
    31261 attataaaaa atgacttgtg tatctgtatt gaatgggatc gcataaggat caccttcata
    31321 ttctactgta tcccaaagct gcggataaaa atccttagaa gtgtcttttt ctacatattt
    31381 ggagagatta gttgcttgct gtgcttctgc tctttgggca acggaattta tgtcctgtac
    31441 aataacatcg ggtgcatttc cagctgttat tgcagataaa gatttagtcc agatgtcacc
    31501 ccatggctga tacttataat ttacatggat ctcgttttgt gattgattga aatcctcaat
    31561 gatttcttcg atagcttctt ttcgtgcacc ggatccccaa aaggaccaga aatcaatatt
    31621 ttttcttcca tcacttgttt tttcagaaga atttgctgca cttgaactgg catcgcttgc
    31681 attacttgat ccgcatcctc ctaataaaaa caatgctgaa cttgccagca accctgtaaa
    31741 caataatttt ttcatatttt acctccaaat tttaatgtaa ccgatgccgg ttttaatata
    31801 acgatatcat tttgttctga aaataacaat ttataaggta tgattctaaa aaaattatta
    31861 atgataccaa tactttatat aattcgcgag cgctttcttt gaatttttta atctttcgta
    31921 gcagaatgag aatgtattaa aaatagatta ggaggaattt tgatgtttag acaccagaaa
    31981 gaattgcagt ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa atatctgcag
    32041 gagctgatcg gcggccaata tggcgagatg agtgtagcta tgcagtacct gttccaaggc
    32101 tggagctgcc gaggagaaga aaaatataaa gatatgctga tggacattgc aacagaagaa
    32161 atcggccacg tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg tgcgcctgtt
    32221 gaagatcaag aagcagcgat gcaaaaagat ccaacaatcg gtgccattat ctctggaatg
    32281 aatccacagc acgcgatcgt agctggctta ggacctcgtc ctgctgacag cgtgggcaat
    32341 ccatggagcg gtgcctatat cattgcgagt ggcaacttgt tagcagactt tagagctaat
    32401 ttaaatgccg aatcacaagg aagacttcaa gtagcacgta tttacgaaat gatcgatgat
    32461 cgtggcgtaa aagatatgct gtctgttctc cttgcaagag atagctatca tcaaaatcta
    32521 tgggaatgtg cagtgaaaga attagaagaa aaagaaggtt caatcttagt cccaagtact
    32581 ttcccgcgtg acaaagaacg aaaagacatt gcttatgact tctataactt ctcagaggga
    32641 gaccaaagta gtgaaggttc ttgggctagt ggcaaagcac tggatggcga aggcgaatat
    32701 caatggttga aagaacctaa gatcgaaggc gctgcaccaa aattagaacc agtaacacca
    32761 aaaatgtatg ggacacctcc attcaagaaa taaatatcta cccgtagaaa cacccgtttt
    32821 ttgttccaca cgctttgtga gcaaaactga ctaaagtcat taatcataac cacccttcaa
    32881 aagggtggtt ttctctagag ctataagctc aaatcaccgg ccagcgccta aagacgctgg
    32941 ctttcatatt ttatgttcaa gccatattgc ctttgccagt tttgcccacc tcttaaaagg
    33001 tttatgtact actccttagc ccttaaaagg atcgtcttac tctcgtacac tcagcttatc
    33061 taacgcacta tcatgttttt cttgttcttg aatgtacttt ttgatggtcg cttcattttg
    33121 tcctactttt gtcacatagt atccttccac ccaaaagtgt cgatacccaa atttatattt
    33181 tagatttgca caatctcttg atgatctcct gaatattggc tcgatattga ttataaatta
    33241 tttttcttct atactttggt gtgaatacaa tgtggtattt acacatctac tttgtacggg
    33301 ctaaactatt tccctttatt gctccacccg catagcaagt ggttttgtat ttcaatcgct
    33361 cggcgcgctc aattaggtct aaagacaata aatctaaaaa cccgctgctt cactgacaga
    33421 aacaacgggt tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa tctattttat
    33481 ttttttgatt ttttataaat ggaaaatgct tcaagaagtc caagaaataa agccgtataa
    33541 aaacaagaac ctatgaaatc tttatttatt agatattcaa cagaaatccc gataacacta
    33601 gccaacaata tatttatcag aagtgtaaaa taaggatgct ttttatagaa tagaactagt
    33661 gttgttttca tagactctcc tttcttcata tttccaatta atcacaaatt cacggcatta
    33721 ctagaaaaat taaaggggcg cagaagtgga aaaatactct tcgactttcc ctacaaacca
    33781 ggccatcttg tgtttaataa ctataccaaa tctaaggtac ctctaaatct aaattccatt
    33841 tgtggcggtg acctccaatt tttaactctt ctatctccca tccagtatac ataaccttga
    33901 tacttcgacc acttatatat cgaacacaca aatacctatc cattgcagga gtactaccta
    33961 acttagttgc tacttgcatc taaataacag ctgaaaagat aggacttact cttagtaaag
    34021 tatctgaact attaattttt aaactatcat ttttatcctc aactacatgc acatctgttg
    34081 caacttcact ttctgtctca tccagataaa tgattaagga atcgggaatt gcaatatctt
    34141 cagctgacgc tggaatagtt gagctaaatt agtcatcata tctttattat aaaataacca
    34201 acagcaaaaa atacaatgaa aaacaaactt ggaactaatg tatcattaat aaatgttgaa
    34261 aaattcagca actcattact gtatgatttt agcagcccaa ccaacatata ccctataaaa
    34321 gctaataaga tggttaggaa tcctttagaa tttttactca ttcatagaca cttccttaaa
    34381 taacttttat tatagaaggt attgtaaata ataatattga caaaaagtga ctcaaaataa
    34441 aaaaaacctt acattatact ggaatgaact gtattgatag tgtacctaaa agctctactg
    34501 acataagatc gtgtaatcgg taaagtaatt ttattaatta tacgggatgt aatcaattag
    34561 agaataaact aatcataaaa ggtactttta cattcataat tgtcgctttg agattctact
    34621 ttggttcact atctgaccca attgcacatt ttcgatcatt acaagtattg gtgtcgttag
    34681 aacaactaaa taaaaactag cggtagattt ttcagaatct atgctaattt ctgtatggat
    34741 gttccagtga tggcaaattt ctcttttcat tgtttttctt gtcgcatgat atgccttttt
    34801 gtgttattca gttaaactat agaaattctt tcctagacag atcttgatcc caaattttac
    34861 gtttttgagc atcgatatag tttaaaatga cattactcat atctaaagag tttagatttt
    34921 ttaggactaa aactgtcagt tgttcttttt cttctttctg ttaaagtatt gtcgcgactt
    34981 ttcgtatacg ttaagatatg tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga
    35041 cttgaggaaa tcacctcaat cattcgttta aaatgctata tattctttag tttaagatac
    35101 ggaaattaat gccatcgacc acactagtag aatgaacgac atttacttgg ccttttttta
    35161 atttcgttat gtttagcata taaaaatatt atagagttta cttacaatgc ttaaaacata
    35221 tactgtataa tctgatcaat tttagccatg tcttgcaatg gtaagttttc tatttttttg
    35281 agttttcgtt ccttaaaatc aagagatttt aattgtgtaa taattacttg tccttttata
    35341 tctagctctt caggtaatga gtaacgtgtc ggtaaattct ttatagtgga agtaattgga
    35401 catatgaccg aaaacattgt tttacggtta aattcataac gagagacgac tagtcctggt
    35461 cgtctttttt gaatttcttt tcctacggag ggatcaaaat caatccatac aatatcacct
    35521 tttttaggaa tataaattct ttcttctgac actataaaat ttctctccct tcaggagtta
    35581 aatcttccca ttcgtctttc tcataatact cggcctcttc cccaccacta aatggatctt
    35641 caatttttgg aactaacgta attgttccat catcagaata cacaacgata tattcttggt
    35701 tttctgaagg tttctttccg ttatcagaag gtaaagttac cactacagaa cttccttgta
    35761 atctagattt tgtaacaagc attatcatca ctcccttaat aaaagtatac tatataacac
    35821 taagtaacgc aaaattcccc tttgtaaact atctagaaaa ttgttttgtt taaagtatat
    35881 cttattggat ataaatagaa ataaattgta gccagttcat tttttcttta actaaaaaaa
    35941 taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat agctaacccc
    36001 ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag acaacaggcc
    36061 atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct gacaaatttc
    36121 aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg atcaaagaat
    36181 gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac tttgctattg
    36241 tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat ccaactcata
    36301 tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca actgccaacc
    36361 agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga taaacatcgt
    36421 taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc tatacattag
    36481 aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt tcttctgttc
    36541 tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct ttctttgaaa
    36601 aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt aatgattttt
    36661 cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa gacaagcggc
    36721 attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat gattgattct
    36781 gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat tttttcctta
    36841 tttgaaaaca taaatttgtc tttttcttca ttccactcat tgggaaaaca caacgatttt
    36901 gcaaaaatgg ttgatgaaag gtcttttttt cttctactgt ctctgcacgg gcattaatca
    36961 tcaattgtcc ttttttaaat ccggtaaatc cccacttcat gatttccgga gtgaggatgt
    37021 tttcttcctc ttttcttaac gtcacaacaa gatttgacgg aaaaacttct ccagctgcta
    37081 attctgctgc ctgcttcggt tgtttcgtct gggcttctcg ataataattc tgaaggagct
    37141 ctgactctag atcaaaaaaa taacgaccac acatcctaat ccctcccaat ctttttttct
    37201 atcatacact gcaacaagaa gttcgcacca acaaaacgct ttcaattttt gtagaactaa
    37261 aaaagttgtg acaagtgaca aactctccag tgaatggtat tctcctgcca actcctcggt
    37321 cacaagtcac aatattcaac aaacatgaga agctgctttt tgaaattgtt ccttgtgact
    37381 cggagtgaaa cggcaggctt acgacctctg ggtaaacggt gttcaaaagc cagtttctcg
    37441 gtattaagcc aaatttatgc aaaaatgtag aaagctattt tcacaaaatt tttcttaata
    37501 ctcgaaacta agcgactttt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
    37561 tgcggtaata agcccaacca aacgaaaaat atgagcagct atttttattt ggctgttctt
    37621 attacttgat gcagggcagc tttttcacaa cctctccact aaacggtgtt caatcagcta
    37681 accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct
    37741 ctaatactca ggtcaaagcg ctgatttcac aacctctaat acacggtgtt ccagaagtag
    37801 cttcttcgaa aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc
    37861 ttcttatttc ttgaagctga ccacttctgt cacaacctct aattattaaa gaaccttttg
    37921 cgaagttaac aatctttctt tgatcaattg aaaattctgc ctaagtagtg atagatcctg
    37981 tgtgactttc aacaataaga tacttccctg gatttcagca atcaacgctt tggcttgacg
    38041 gtctgcttct tcatcggaca gtttttcttc agaaagtgcc aggatatttt gtttcgtata
    38101 cttgacccat tgattgacta attctagtag caacagacgg tattcttcat ccactgtact
    38161 caactcaata atcaaattac ctacaggaca gccatgataa gtgatctgtg tctcatgatc
    38221 agcgaaaatc cagtccaaca tttcttctaa tgcttcttct ggctgttttc cagcatttaa
    38281 gatgtcttcg aacaattctg tctgccatac gtccatatgc gcatggatca ccgctagaca
    38341 aacttctttt tttgaagaaa agtaataata aaattgacct tttcctgtat cagaagctgc
    38401 tagaatatct cggattgacg tttcatgata gcctttttta taaataagat ctttggtcgc
    38461 ttccaagatc ttttcctttg ttttcaatga tattctcctt tttcatttct catagtccta
    38521 tattatacag taaattcatc attttttctt cttaatcgtt taaaaaagac tgtgccaaat
    38581 tatctgacac agccctcact ataaaactgt tctcatatag ctagatgttc attgattcaa
    38641 gaactgcgag gacaaaagca attcctatct tatgatagtt gctctaatac ttcctctgtt
    38701 gtcatcactt gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat tgcttcttgg
    38761 ccaggagcac caattgcatc tgtcgctacc acaacattga atccactctc taccaattca
    38821 cgcatatggg aatcgctaca caagtttccg ttcattccac caagaattac tgtgtcgatt
    38881 ccattttttc tcaattggag cgccaaatca ttgctttctg gtccaaagat tttatgagga
    38941 gagctgatga tcgtattttc atccaaataa ggtttcaatt cttcgataaa atcggctcct
    39001 gaaccttctt ccactgattc atattgactt tttctagcaa acatctgctg ctcaagcatc
    39061 atatgttctc ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa ataatgagga
    39121 gaaataaaga gtttgtatcc tttttctttt gcttgtttta ccatttcaat catattttcg
    39181 atcgtattgt atttttctaa gatatctttt gtcaaaccaa agccaccgcc tgctggtttc
    39241 aagaactcga cttgcgggtc agtgagaact actgctgtat ttttattatt gaacatggtg
    39301 gattctccat tcgttattta ttttaataca tcatttagag cttcactcat tgtcggatga
    39361 gaatagattt gatctctcaa taacgtataa ggaagttttc cattcatagc tagcgaaatc
    39421 aagttgatga cttcatgaga atcttccgca tagatacttg cccctagaat caagtcagtt
    39481 tcagggtcga ctaaaacttt gaatacacct ttaggatctt ctaatacttg tgctttaggg
    39541 acaccgcttg tcatatactt gaataatttg taattgacgc cttgtgcttt ggcttgtttt
    39601 tctgttaaac ctacattgga taaagtcgga gtgataaata cactgtaagg cacagctttc
    39661 cggttattcg ttgttcgttt attctcgccc ttcaactgat ccataatgat tcgataatca
    39721 tctagagaga tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg
    39781 tctgcatctg tacgaaggta ttcatccaca gcgattgctc cgcgatctgt tagtttaaca
    39841 tctgtatttt ctaatccaag tgatgctgta cttggttttc ttccagttgc agccaaaact
    39901 tttgacgctt ggattgttac ttcttggcca tcttctgtaa aggtgacggc agctgttgat
    39961 tcttggtctg ctacttgatc tacagataca cctagatgga attcgattcc cgcatttgtc
    40021 atgtcttcta aaatcatttg cgcgatatct tcatcttcac gtggaagaaa ttcagaatgg
    40081 gcatcaagaa cgacaacttt agatccgtat tcattgaaca ttgatgcaaa ttccaagcca
    40141 atatatccag ccccgatgat cactaatttt tccggtaatt gttcctgatc catcgcttgc
    40201 gtagaatcaa tcaagtagcg acttgtattc aatcctttga ttggtaaaat gattggttca
    40261 gaaccagtat tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc attattcgtc
    40321 acttcgattt gatggttaga aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc
    40381 tcatcgctga tcatatgata atttttggcg cgaagcatgc ccgtcaattt ttctttccga
    40441 gcaacagcct ctgtaaagtc gattcctctt tctccattga aaatcaatga tttagaagga
    40501 atacagccga tgttgataca tgttccgcca tacatacgtg ttgacttttc aacgactaaa
    40561 accgattcac cttttgaagc taacgccttc gctaaagtct taccgccctt tccaaaaccg
    40621 atcacaatat tttgatattt ttccatcatt tttccctcgc ttctttttta cctaccgatt
    40681 ggtacaaaaa tatgataacc gataattgtc tattctccaa atcatctgct ttatttgtca
    40741 aaaaaaacca gacaaaaagc ggtcctttca cgtttttagt ctggttcgat ttcctttatt
    40801 cttctaaaca atcgttactt ctacatcgat atttcctcta gttgctttgg agtaagggca
    40861 gaattcatgc gcatcttctg caattttttg ggcttcttct tcgctgatgc catcgatatg
    40921 cacttctaat actacaccga tctgatagcc gccatcatct aaattataaa gagagacacg
    40981 tgcttttact gtggactctg ctgtcattcc tgcttctttg atcatatgtt gtaccgcccc
    41041 attgaaacaa gaagcataac ctgctgcaaa taattgttct ggatttgttc cttctgcatg
    41101 gattcctgga ggagtgattt tcatatgcat cgagccattt ggtgcctcga cttctccatt
    41161 gcgcccgcca tggttgatca ttgctgtttc gtatgctttt ttcattgtac tgctcctttc
    41221 gattgaccat ttgctacttg gctataatta tagtgtatga caagtaaggc gctttctaca
    41281 aaaattctgc ttattcgaat accagaatgc cgtcttctac tatttttcca aaaaacaaaa
    41341 aaatactact cgtgaacaac agcgtcatat ttgttccaaa ataaatagca atcttttcta
    41401 cataatcttt tatatgattg attttaagat acatatatac cagaaagttg accccataaa
    41461 ccaatactgt taaaaaaata accatattcg ttaccatttt ttcctccttt tgctggtctc
    41521 cctaccagca ctcgatttaa ccataccact ttttttgccg tccaagcaac tagcctttca
    41581 aaaagactgg tttattctgc tgttaatgat tcaagatcag aaaaatgtcc gaataatttt
    41641 gctaatttta ctaacgccaa cttgttcttt ccgaattcat cattttctat ctgtaatact
    41701 agtaacggct catgtaagct taaacggatc aaaaaccagc cgtttccgta tggctcttct
    41761 agatcatatc gaataccttc ttcattttcc gggtcgaagt tcatcccttt ttcttcttcc
    41821 aacagtcgct tcatttcttc taatatcttg tttccttgtt cgcgtggttc atcgctgaac
    41881 aattttaatc ggatttcttg tacttctacg ggctgtttca actctttgat ttgttcatct
    41941 aatgtgatac cttttctttt taattctggc aaaagcatca ggatctttgc gatgacatat
    42001 gcaccatcat ccagaaaata gttttccttg aatgcagcgt gtccgcttgt ttcgatggct
    42061 agctgtgtat ctttcccaag acggttaagt tctaatgctt tattgattac atttcgatag
    42121 ccggaaatat aacgaacttg gatgcctcct ttttcttcaa tgaatctttg tagatgagaa
    42181 gaagtagggg aattcgtcac gattcttgtt cctggatgct cttttaatac gattgtactt
    42241 aatactgcga tcaaattatt tcgattgatc actgttccag aagcactgac aactgcagaa
    42301 cgatccacat ctgtatcgaa aatgatccca agatctgctt catttgctaa aacagcctgc
    42361 ttgatacttt ccatcgcttc tttattatca ggatttggga tatgattagg aaatcggcca
    42421 tctggttcca aaaattggga acctgttgaa tccgcaccta agctattcaa tactttttca
    42481 gcaaagaatc caccagcacc atttccggca tcaacaatga ttttccaacc atttagtggt
    42541 ttgctttcta gtgttccaat ccccttttta atttttgtga ctaaatcttg cgcataaggt
    42601 gtcaaaaggt ctgctttttc tacgcttcca gtttgttcgc tttttatttt ttgctgctgt
    42661 gataggataa aagcaatatc ttctttttct gctcctcctt ctgaggtaaa gaattttatt
    42721 ccattataat aatagggaag atggctagca gtcagcataa ccgcagcatc gcacgagaac
    42781 tgttcaaatt gagtggacat aaacatagca ggcgtagtag ctaattcgaa atcgatgaca
    42841 tgtatccctt gttctttcag actttggatc aacgcctctt tcagtgctgg tccgcttatc
    42901 ctactgtcat gaccaatccc aatcgtccat tttttatttg gatcggttga tttcttcttt
    42961 ttctttatcc agtttgctag tccccagccg atctttttta ccatgtctgt cgtcaagttt
    43021 acttcatatc cttcaactgg taatgcgatt cctcgaatat ccgatccatt ttgcagcgct
    43081 ttcatatctt tcatataaaa gtcctcctca tttattccct cattttttcc agcttttctg
    43141 caaatcgctg tgcttcttct tctgagcggc aaataataat gaatgtatct acaccggcca
    43201 tcgttccgac aacttcaggg taagccactt catctagcca attcgttacc acatcggcac
    43261 catttcgttc agtcaatacg ataaccatga actgaacaga ctggatacgc aaaacctcgc
    43321 gtctaattgc tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg ttaaataatg
    43381 tatagcgtac tattttgttt tcattttgac tttttactaa cttcatttct cgaatgtctc
    43441 gagaaatcgt tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca agcagctctt
    43501 cctgtgtccc aataggttgt tctgtaatga tctggcggat cagtgcttgt cgctgtttct
    43561 ttttcatatt agacacctct caatgttgat tatagcaaaa gataagtaat atttcttcat
    43621 attttggtat tttttacttt tattttttgt tttcattcag gttgctaacc tttttctatt
    43681 ttaacacgca aaatcgtctt cattttttca ggtgctgatt ttcttggatc gctgatgtaa
    43741 atctctttgt gttctttaga agctctttca tagccttctt ttgcgatgaa tgcttctaat
    43801 ttctcaaacg ttgctggttc ttcggcatag ggcccgacgt gtaagatttg ggcacaaaga
    43861 ccttcttcca tctttttgaa tactacttgt tctttcaagt cttccggaag tttgctagaa
    43921 cgctgaatgg cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg gatcatcaat
    43981 tgataagaaa aatgttcttt tttcatcact ggttcatgca aatacttttc ttgcagtccc
    44041 cattgtcctt ccagtggata gacagtatac atctgataat caggaatcag ccaatttttt
    44101 ttaggcgaca tgcgtattgc atagctgact gcatatagac aggctactct tcgtttaaag
    44161 tcttcctgat tcgggtcgcc gatccctctg atgctaaaga atgatttttt aggaacctct
    44221 agtatttgcg ggcttttttt catactatag ctcttgtctt tacgccattc aattttttcc
    44281 atttttcttc ctccgcgaaa ttccatttag ttatttatta tcatacctct ttttgagtga
    44341 cttggctaat cgtgttgaca ctatttttca gtttgtgcta gaataagtga agtaaataaa
    44401 tcgtacagat aacacgtttc ggcgtgtcga gagagaggcg gtttctattt ttataataga
    44461 aatcactctc tttttttacg aaatttttta gaggtgatgt aaatgacaaa acaaaaacag
    44521 ctgcttttag tacctgcagt aattgcaaca gggatgatgt cttttgcggg cgtattgatc
    44581 gagacggcca tgaatgtaac tttcccaact cttatcagac agtttgattt aacgactgca
    44641 caagtccaat gggtaacaac gatttatctt ttgatgattt cgatcattgt tccgctgtct
    44701 acttatttga atcgcaattt tagtctcaaa aaactctttt tagcttcaaa tctgcttttc
    44761 ttggcaggta taatgatcga tttcttctct cctaccttca tggtcctatt gtttggtcga
    44821 ttgctccaag gtgcagcgac aggtatcgct cttccgttga tgttccatat tatcttgaca
    44881 tttgcccccc tcaatcgacg aggcgcgatg atgggtgtcg gtactttgac aacggccatt
    44941 gctccagcaa ttggccccac ttatggaggc attatgactt cacattttac atggaatcat
    45001 atcttcctgt ttcttgtgcc cgttttgttt ctttctttga ttatcggctg gtttgctatt
    45061 cccgagatgg cagtacaaaa aagtggttat ttggattgga taagcgtttt aggaatttgt
    45121 cttttgttta gtggaagtct aacatttttg agtacgttgg gaacagtttc cagctggatt
    45181 gctttaatca tcggcggtat tggctggttc cttttttact atcgaagtaa aaaagtggag
    45241 catccactga tccgactgtc tattctttca aataaaacat tccgcttgtt tttgattggc
    45301 ttcttagttt atcagttttt attacttggt gtatcttttg tcttgccgaa tttccttcag
    45361 atcgttcaag gaagtagtcc ttttgtcgct ggattagcga tgcttcccgg cgcagcggtt
    45421 ggtgctttgc tttctccttt ttccgggaaa atgctggatc aatatggtcc gaagaagcca
    45481 atttttgctg ggctgattct ttctttgatt ggttggctag cattattcct actcatagga
    45541 cacgcttcag tgggtgtatt gattgcttgt catgtgtttt atatgatcgg tatcggatta
    45601 tcttacagca atatgatgac caccggaatg aatgcactga atgaagaact acaaggagac
    45661 gggaacgcaa tgttcaatac cttgcagcag ttttctggcg ctgtggctac ttcattagta
    45721 gcggtcatta tcaattacgt ccagcatcat acttcccata actatgaagt gtctactaca
    45781 ttaggatcaa aagcagcatt aggcgtcttg ttgctgttgc ttctcgtaag ctttgctcga
    45841 ttcgcctatt atctgttctt tgctaaaaaa gcgtagattc catatacggc aggtgttgca
    45901 tctgccgttt ttttgtgtga aaatggttgt ttgtaaaagg aggctttttt atggaaaaaa
    45961 ttgccattat cggtggcggg atcattggta tgaccctagc cagttactta gatacatcaa
    46021 aattcaatgt tgctttattt gatgaaggga caggacaggc aactaaagct agcgctggga
    46081 ttatctcccc ttggttgtcg aaacgcagaa acaaacaatg gtatcgtctg gctaaagacg
    46141 gcgctgcttt tttccctaaa cttataaaag atttccaact tgatcaaacg atttacgcac
    46201 aatcagggac acttttgctt cgacctgaaa aagatttagc tgaattggct gttcttgccg
    46261 agaaacgaaa agaagaagcg ccagaaattg gaaacatcga atggctttca cctgaacaga
    46321 catctcaagc acttccgtta ttgaaaccag cagtttcact gcgtatcagt ggtggtggtc
    46381 gtctggatgg aaaagcatat ttagaacatc ttggtcagcg tttagcacaa aaacaaatgg
    46441 agcagatcca ggcaaaagtc actttgcata agaagagcga ttcttggctc atcaagggac
    46501 cgtttggaga atacacagct gatcgtgtaa ttttgacacc tggcccggca cttaagcctt
    46561 tattacaaga attaggctat gctgcagata tacgtccgca aaaagggcag ctcttagcat
    46621 ttcagacatc ttataagatg agtcaagact ggcctgttgc tatgcttgac ggagaagctg
    46681 atttcattcc tttcatggat ggaaagatcc ttttaggtgc tacccatgaa aacgatcaag
    46741 gctgggattt ggctcctact agcgaagctt ataagggatt gactaagagt acagaacagt
    46801 tcttgactga tccagatcag ataaaaaact ggccatacga gttccgtgta ggtacccgtg
    46861 cttatacgtc agatttttcc ccatttttcg gcgtactgcc agatgatcca agcttactgg
    46921 ctgctagcgg tctaggctct tctggcttga ccacaggtcc ttatatcggc tatctattag
    46981 ccaacttctt aaatactggt cattggccta cggaagatta tcaaaaggac atctccacct
    47041 atattacgaa aaatccatca tagaaaaaca gctttttcaa gtaaggatcc cgagcgatat
    47101 tgcgcatgga ttacttcttg aaaaagctgt tattttagtt caatttctga atcaaattct
    47161 ccaaactttc ttttgcttca tctggcaaga gatcttttcc tatctgagct tcaatgaatc
    47221 gtaaacgaat atcaagtcgt tgtttcacca gatttttata ggacgtatcc tcaaaagaaa
    47281 caggaaaatc tggtataggt aaatagtgga tgtcttcaaa tggaccaaac gacttgaatt
    47341 ctgccgtttg ttgcacgatt ctttcaacga ttgacaatgt cgtttcaggt ttgatttttt
    47401 tatcatcgaa atatccagta ttcaggacat agcaggctgt ttgctgtttc aaaaacaact
    47461 ctcgaaaagc ttgatagtct tccctaagag gatagacacg gaaaggatta gcaaatggtt
    47521 caatgactag ctcatctagc tgtctttttg tagtgatatt ttccgcgtct gatcgttttg
    47581 ttgctaatgt tgcaccaaaa gacgctgcta aaactgggtc attgatcttg ataattggcg
    47641 gaagcgaatc atctttcatg atccaaaaaa cagcatcgat cttttcgcta agatgatcga
    47701 ctcggtcggg tgtcgcataa cgtgatttga ctgtccgccc atttccgttt cgtatatctc
    47761 ctgttactag gactttttct cctttacttg tacatgtcac gcccacattc tgacaagtaa
    47821 ggaaagaaga aatggccgga tcatcagacg gatagtcttg tgttttatca aagtaagaag
    47881 gttccaaggc agtagcaaaa ccattttttc gagaaataac gaaagcatcg tcatgcaaga
    47941 tcgaaacaga gtgaccttcc gtatgttttg ctaaggtaat cgttgatttt ccagaaccgg
    48001 acaacccaaa tacagccatc gtataggtct ggtcattact agcatattgt ttcatcccac
    48061 cgtggcaggc aataaagcca ttgcgatttc ccaatgccca agcaagcgtc aaagtcgctt
    48121 tttttaattc tccaaagtaa cgtaatccta gcacagccgc tgtattatgg agagcatcaa
    48181 acaaaatgag tccatttgga aatgtttcat gtgtccattc gggatcacaa aagatataaa
    48241 gatctccctc atcaaaggaa acagactttt gataagtctc taagatcttc gaattcgctg
    48301 gctgaaaatt cagcaaataa gaatacaaat tgttctcaaa tccttctgga atcagcaaat
    48361 gactttgcac agaaaattct tcagataggc caacgattac ttttgtatgg taaaactggc
    48421 gcttgcgact ttgatacaca gcttctctta aaattttctc gtatttgctt tgttccttgt
    48481 ctaagtcctc aaagatccgc cgagcacttg ctgttcttcc cacaatgctt ccatcattga
    48541 atactagtgt cctaacatca gctggtaaat ctagttcttc agcatgtgag attgttaaat
    48601 ctgtgatgat cgttcctgga ctcccttttg ctagttgata agctgtcttc ctatcaaaaa
    48661 cttcatgaac attattaccg taaaatgtcg tttctacaat cgatcgaata ctggataaca
    48721 gtggatttcc acttgtgatt tcgtttaatt cgagactctc ttttgtactc atggctcctc
    48781 ctcattccct aaacaaaaaa acgttttctt tattatacat tattcgagtt tttatttgta
    48841 ctgctgcgct gtgggaagca aacgagttat ttttcacaca aaaaaaccga gaacttgaat
    48901 aaacaagttt ctcggtttca tcattttcct tttatcggct tagtaccagc cgtttgctaa
    48961 ccagaatgtt ttagcagcat cccatgaacc ataacgagat gttacatatt gttctgctac
    49021 acgttcttga ttggcagctg aatagtcgcc gtttaagtat gaagcatcta attgataacg
    49081 gccgatgtaa cgtccgttag tagctgtgta tgaaccgcct gattcttttt gtgcgatcca
    49141 ttcttttgca gagcttgaag ttggcgctgt tgcagcagct ggggcagcag gagctacttc
    49201 aactgtttca acaggtgcaa cgtatgtatg ttcttgcgtt gctgtttcag ttgtagatgc
    49261 tgtattttct gttgttttgt tttctgttgt tgctgaagaa tcatttgaag ttgggattgt
    49321 taattgttca ccaacataaa tgcggttgat gtttgcgatc gaattttttt cagcaattgc
    49381 atcgatcatt gagttgtctc ctgcaaattt ttgtgagatc ttagataatg aatcacctga
    49441 ttttactgta taaacttcat cagcgtgagc atttgtagtt cctacaaaca ttgcagtagc
    49501 accagcagca aaagttgtac ctaaaacaat tttcttaaat gaattcatat taaactcctt
    49561 tttttctccg ttttttattc tttcgacgct attaacttta tcatttagaa atatttcata
    49621 ggtaaaagaa acatgacatt taatgacaaa ataacgaatt atttaatatt tgtaacattt
    49681 aaacaaatat ttttttgata caaaaccata aaagccttgt tgtataagca aagtaatcgt
    49741 ttttctatgt ttttttattt ttgaatgaaa aacgcagatt ctgtaacatt cactgcaata
    49801 atccataaaa aaaagcaaaa aaatttttga tttttatcaa attctcttaa aaaaatgtaa
    49861 aaaatacaaa aaaaccagta attgttacaa caattactgg acaaaaactt tgaataatgt
    49921 taactatttt t
//
LOCUS       DANKMN010000010        47836 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000010 DANKMN010000000
VERSION     DANKMN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(70..1491)
                     /locus_tag="PW224_000595"
     CDS             complement(70..1491)
                     /locus_tag="PW224_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2907309.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="PW224_000596"
     CDS             complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="PW224_000596"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2907310.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3902..5158)
                     /locus_tag="PW224_000597"
     CDS             complement(3902..5158)
                     /locus_tag="PW224_000597"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL2907311.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5270..5475)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5738..7038
                     /locus_tag="PW224_000598"
                     /pseudo
     CDS             5738..7038
                     /locus_tag="PW224_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(7107..7706)
                     /locus_tag="PW224_000599"
     CDS             complement(7107..7706)
                     /locus_tag="PW224_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2907312.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7961..8842
                     /locus_tag="PW224_000600"
     CDS             7961..8842
                     /locus_tag="PW224_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907313.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8855..10465
                     /locus_tag="PW224_000601"
     CDS             8855..10465
                     /locus_tag="PW224_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL2907314.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10542..11201)
                     /locus_tag="PW224_000602"
     CDS             complement(10542..11201)
                     /locus_tag="PW224_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2907315.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(11484..12149)
                     /locus_tag="PW224_000603"
     CDS             complement(11484..12149)
                     /locus_tag="PW224_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL2907316.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(12296..12901)
                     /gene="dhaL"
                     /locus_tag="PW224_000604"
     CDS             complement(12296..12901)
                     /gene="dhaL"
                     /locus_tag="PW224_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL2907317.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(12929..13939)
                     /gene="dhaK"
                     /locus_tag="PW224_000605"
     CDS             complement(12929..13939)
                     /gene="dhaK"
                     /locus_tag="PW224_000605"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL2907318.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(13914..14360)
                     /gene="dhaM"
                     /locus_tag="PW224_000606"
     CDS             complement(13914..14360)
                     /gene="dhaM"
                     /locus_tag="PW224_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL2907319.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            14463..14783
                     /locus_tag="PW224_000607"
     CDS             14463..14783
                     /locus_tag="PW224_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL2907320.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(14895..15764)
                     /locus_tag="PW224_000608"
     CDS             complement(14895..15764)
                     /locus_tag="PW224_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2907321.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(15860..17164)
                     /locus_tag="PW224_000609"
     CDS             complement(15860..17164)
                     /locus_tag="PW224_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2907322.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(17161..17670)
                     /locus_tag="PW224_000610"
     CDS             complement(17161..17670)
                     /locus_tag="PW224_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907323.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(17845..19980)
                     /locus_tag="PW224_000611"
     CDS             complement(17845..19980)
                     /locus_tag="PW224_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2907324.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(20063..21718)
                     /locus_tag="PW224_000612"
     CDS             complement(20063..21718)
                     /locus_tag="PW224_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2907325.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(21720..22034)
                     /locus_tag="PW224_000613"
     CDS             complement(21720..22034)
                     /locus_tag="PW224_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2907326.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            22204..22464
                     /locus_tag="PW224_000614"
     CDS             22204..22464
                     /locus_tag="PW224_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL2907327.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            22478..22792
                     /locus_tag="PW224_000615"
     CDS             22478..22792
                     /locus_tag="PW224_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL2907328.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(23522..24076)
                     /locus_tag="PW224_000616"
     CDS             complement(23522..24076)
                     /locus_tag="PW224_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL2907329.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(24202..26131)
                     /locus_tag="PW224_000617"
                     /pseudo
     CDS             complement(24202..26131)
                     /locus_tag="PW224_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(26437..27153)
                     /locus_tag="PW224_000618"
     CDS             complement(26437..27153)
                     /locus_tag="PW224_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2907330.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(27154..28977)
                     /gene="glpO"
                     /locus_tag="PW224_000619"
     CDS             complement(27154..28977)
                     /gene="glpO"
                     /locus_tag="PW224_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL2907331.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(28991..30487)
                     /gene="glpK"
                     /locus_tag="PW224_000620"
     CDS             complement(28991..30487)
                     /gene="glpK"
                     /locus_tag="PW224_000620"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL2907332.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(30623..32083)
                     /locus_tag="PW224_000621"
     CDS             complement(30623..32083)
                     /locus_tag="PW224_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2907333.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(32073..33368)
                     /locus_tag="PW224_000622"
     CDS             complement(32073..33368)
                     /locus_tag="PW224_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2907334.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(33700..34377)
                     /locus_tag="PW224_000623"
     CDS             complement(33700..34377)
                     /locus_tag="PW224_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL2907335.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(34407..35498)
                     /locus_tag="PW224_000624"
     CDS             complement(34407..35498)
                     /locus_tag="PW224_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL2907336.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(35599..36033)
                     /locus_tag="PW224_000625"
     CDS             complement(35599..36033)
                     /locus_tag="PW224_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2907337.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(36617..38074)
                     /gene="ascB"
                     /locus_tag="PW224_000626"
     CDS             complement(36617..38074)
                     /gene="ascB"
                     /locus_tag="PW224_000626"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2907338.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(38071..39966)
                     /locus_tag="PW224_000627"
     CDS             complement(38071..39966)
                     /locus_tag="PW224_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2907339.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(40024..41799)
                     /locus_tag="PW224_000628"
     CDS             complement(40024..41799)
                     /locus_tag="PW224_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2907340.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(41849..43345)
                     /locus_tag="PW224_000629"
     CDS             complement(41849..43345)
                     /locus_tag="PW224_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2907341.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(43539..45161)
                     /locus_tag="PW224_000630"
     CDS             complement(43539..45161)
                     /locus_tag="PW224_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2907342.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(45489..46802)
                     /locus_tag="PW224_000631"
     CDS             complement(45489..46802)
                     /locus_tag="PW224_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2907343.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(47047..47271)
                     /locus_tag="PW224_000632"
     CDS             complement(47047..47271)
                     /locus_tag="PW224_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907344.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(47291..47713)
                     /locus_tag="PW224_000633"
     CDS             complement(47291..47713)
                     /locus_tag="PW224_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL2907345.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
BASE COUNT        13635 a        10136 c         8211 g        15854 t
ORIGIN      
        1 aaaaactatt cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga
       61 cttatttttt tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt
      121 gaagtgattg atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa
      181 tgcatcactc aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat
      241 cagcatgcag ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg
      301 ttcgaattct ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac
      361 gatacaataa ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc
      421 cattacacca taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt
      481 tctagatacg ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc
      541 acgagctact tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag
      601 cacaccaata aataccatga agctgaagat atttccgatc acttctggta agcctaggat
      661 ttgacagtca atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac
      721 gacatttgct acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat
      781 aaatacgcct tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact
      841 catttcgatc ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg
      901 ttccaagtca gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag
      961 tccgactttg aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac
     1021 ccctagccat acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat
     1081 cgtgatgatc cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag
     1141 ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat
     1201 acctgggaac atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag
     1261 tgctgttttt acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc
     1321 ggaaaccatt gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa
     1381 tgtccaaccg actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt
     1441 cagaccgata aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac
     1501 tctccttgat tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa
     1561 acaattcagc gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa
     1621 gacaacctca aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa
     1681 catgcgtcag aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt
     1741 gcttcgcttg ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct
     1801 ttgatttttt cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg
     1861 ctggcacgca atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt
     1921 tggttcacaa attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa
     1981 gttaagaact cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca
     2041 tggttcaatt tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct
     2101 accatattga aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta
     2161 aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt
     2221 aatttaccat atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct
     2281 gctgttgccc ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct
     2341 gcgccttttt caaatacata tgtcgcaaag taagagataa cgtctctcat gcggatatct
     2401 ttgatgacga taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt
     2461 acagattctg cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct
     2521 ttgttttctg taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg
     2581 aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg
     2641 tgtaagtaga agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg
     2701 ataccgtcga ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt
     2761 gttttttcag ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag
     2821 ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct
     2881 tttagccatg aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt
     2941 tttccgctac gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac
     3001 agattcatga tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact
     3061 agttcaggtg tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa
     3121 agtccttcta agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag
     3181 ctcattaatt ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa
     3241 gattcatagg caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat
     3301 ggcatcaatg tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg
     3361 gaatgggtac gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg
     3421 tgttcaaaac tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg
     3481 tagttttgac gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt
     3541 tggccgttct cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat
     3601 gattcactca tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta
     3661 cacctgttct tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat
     3721 gtatcaatta tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac
     3781 aaatgtaaac tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg
     3841 gtttcaaaat tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat
     3901 tctaatcaat tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact
     3961 taccatcgaa ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt
     4021 taatacttat agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt
     4081 ttgtggaaac aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca
     4141 ttttaccgaa tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca
     4201 atgcttgcgt tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa
     4261 cttcttctgc caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg
     4321 ctaagtcttc gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat
     4381 cgcgatcatc ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat
     4441 caagccaaac ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca
     4501 atgggatcgt taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta
     4561 atccagctgt gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg
     4621 ttttatgcag atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg
     4681 agattcctac ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga
     4741 agtttttacc gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt
     4801 tgttcacaaa tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg
     4861 cgtccacgtt atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc
     4921 ttgggtcgcc gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca
     4981 attggaatct tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag
     5041 ttggatcaac gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt
     5101 cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt
     5161 tactgacctc cgtgtagttt ttttatgaaa gcaggaacct gctagcatgt gtaattaatt
     5221 atttcttcag gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga
     5281 aatctcgcgg taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta
     5341 acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg
     5401 tactggtttg cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc
     5461 gatcttcgtc ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca
     5521 acagaaaaag gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt
     5581 ccgaacattt tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca
     5641 atttatcgag aagtcacttt actttgcctc ttattctttc ttcgttacaa taaggaatgg
     5701 gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat
     5761 tttaggagcc acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat
     5821 ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     7741 tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga aagtatcaag
     7801 ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat attatgtact
     7861 aaaataataa aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc
     7921 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
     7981 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
     8041 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
     8101 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
     8161 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
     8221 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
     8281 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
     8341 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
     8401 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
     8461 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
     8521 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
     8581 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
     8641 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
     8701 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
     8761 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
     8821 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
     8881 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     8941 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     9001 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     9061 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     9121 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     9181 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     9241 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     9301 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     9361 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     9421 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     9481 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     9541 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     9601 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     9661 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     9721 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     9781 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     9841 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     9901 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     9961 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
    10021 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
    10081 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
    10141 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
    10201 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
    10261 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
    10321 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
    10381 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
    10441 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
    10501 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
    10561 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
    10621 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
    10681 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
    10741 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
    10801 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
    10861 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
    10921 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
    10981 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
    11041 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
    11101 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
    11161 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
    11221 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
    11281 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
    11341 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
    11401 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
    11461 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
    11521 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
    11581 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
    11641 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
    11701 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
    11761 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
    11821 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
    11881 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
    11941 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
    12001 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
    12061 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
    12121 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
    12181 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
    12241 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
    12301 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
    12361 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
    12421 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
    12481 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
    12541 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
    12601 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
    12661 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
    12721 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
    12781 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
    12841 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
    12901 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
    12961 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
    13021 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
    13081 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
    13141 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
    13201 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
    13261 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
    13321 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
    13381 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
    13441 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
    13501 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
    13561 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
    13621 ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag
    13681 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
    13741 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
    13801 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
    13861 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
    13921 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
    13981 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
    14041 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
    14101 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
    14161 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
    14221 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
    14281 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
    14341 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
    14401 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
    14461 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
    14521 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
    14581 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
    14641 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
    14701 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
    14761 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
    14821 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
    14881 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
    14941 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
    15001 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
    15061 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
    15121 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
    15181 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
    15241 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
    15301 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
    15361 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
    15421 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
    15481 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
    15541 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
    15601 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
    15661 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
    15721 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
    15781 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
    15841 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
    15901 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
    15961 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
    16021 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
    16081 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
    16141 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
    16201 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
    16261 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt
    16321 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
    16381 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
    16441 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
    16501 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
    16561 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
    16621 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
    16681 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
    16741 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
    16801 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
    16861 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
    16921 actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
    16981 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
    17041 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
    17101 aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga
    17161 tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt
    17221 tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg
    17281 ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc
    17341 aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga
    17401 ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt
    17461 cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg
    17521 gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt
    17581 ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga
    17641 tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg
    17701 taggagttta ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag
    17761 aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg
    17821 ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat
    17881 tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc
    17941 aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat
    18001 ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc
    18061 ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc
    18121 tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg
    18181 cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc
    18241 tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg
    18301 gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg
    18361 attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa
    18421 ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt
    18481 taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg
    18541 tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg
    18601 atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg
    18661 taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc
    18721 gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact
    18781 ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg
    18841 aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt
    18901 tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa
    18961 aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt
    19021 ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc
    19081 ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc
    19141 taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag
    19201 caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat
    19261 gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac
    19321 atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt
    19381 ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc
    19441 aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt
    19501 atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag
    19561 ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt
    19621 tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt
    19681 tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt
    19741 ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc
    19801 caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga
    19861 ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat
    19921 tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat
    19981 acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg
    20041 aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag
    20101 tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga
    20161 ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc
    20221 tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga
    20281 atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta
    20341 gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg
    20401 ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca
    20461 attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat
    20521 cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct
    20581 ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat
    20641 cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac
    20701 ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc
    20761 cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg
    20821 ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag
    20881 catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac
    20941 ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac
    21001 caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt
    21061 tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag
    21121 acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg
    21181 cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc
    21241 cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca
    21301 ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg
    21361 gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa
    21421 tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca
    21481 cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga
    21541 caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg
    21601 caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc
    21661 gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg attttcatct
    21721 tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca
    21781 tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa
    21841 atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca
    21901 tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac
    21961 gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga
    22021 aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca tggggtatat
    22081 taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt
    22141 tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt
    22201 gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt
    22261 cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta
    22321 agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa
    22381 aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg
    22441 atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga
    22501 tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc
    22561 agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc
    22621 caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg
    22681 tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt
    22741 ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga
    22801 ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt
    22861 ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag
    22921 ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag
    22981 cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag
    23041 aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca
    23101 gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa
    23161 gaaaaatttt gtgaaaatgg atttctacat ttttgcataa atttggctta ataccgagaa
    23221 actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat
    23281 tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc taataccgaa
    23341 ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta actccgaatc
    23401 acaagaaata atttcaaaaa caacttctcc tgtttttggt tcggttggct tattatcgag
    23461 aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc acaccccttt
    23521 tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa
    23581 gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa
    23641 gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg
    23701 ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt
    23761 cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca
    23821 aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg
    23881 ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa
    23941 caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca
    24001 gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt
    24061 ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc
    24121 ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca gacactgata
    24181 ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg
    24241 tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc
    24301 acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg
    24361 gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt
    24421 tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt
    24481 ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctgacgtt cttttttatt
    24541 ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc
    24601 tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg
    24661 agcaatccac aaagcttggc tttctcctgt ttcttccaac ccgcaactga tatttaggac
    24721 gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga
    24781 atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag
    24841 attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct
    24901 catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca
    24961 tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga
    25021 aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca
    25081 tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg
    25141 ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat
    25201 gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc
    25261 taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca
    25321 ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca
    25381 atgccgccag gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt
    25441 cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat
    25501 cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt
    25561 cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga
    25621 atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac
    25681 gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact
    25741 cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt
    25801 attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt
    25861 cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga
    25921 tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc
    25981 ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga
    26041 ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa
    26101 attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg
    26161 tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc
    26221 atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag
    26281 cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac cagctacact
    26341 cacaaaacat accccattct tctaggtctt atttattttc cacttaacta tagcaagaca
    26401 cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc
    26461 taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc
    26521 tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg
    26581 agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa
    26641 tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa
    26701 cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg
    26761 tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca
    26821 atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc
    26881 aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga
    26941 gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac
    27001 agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt
    27061 taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa
    27121 ctcccccatg atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag
    27181 atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt
    27241 ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc
    27301 tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat
    27361 ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg
    27421 tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat
    27481 ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac
    27541 tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc
    27601 gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc
    27661 tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc
    27721 tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct
    27781 gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata
    27841 ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc
    27901 accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc
    27961 ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag
    28021 ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc
    28081 tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg
    28141 ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa
    28201 atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg
    28261 tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac
    28321 ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa
    28381 gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt
    28441 atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag
    28501 cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt
    28561 ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac
    28621 cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc
    28681 tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc
    28741 tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga
    28801 gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga
    28861 tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc
    28921 caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg
    28981 gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa
    29041 gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt
    29101 cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc
    29161 ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt
    29221 ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct
    29281 tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact
    29341 gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc
    29401 ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa
    29461 cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca
    29521 tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta
    29581 aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg
    29641 ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca taagtattct
    29701 tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca
    29761 tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt
    29821 ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct
    29881 cctgatccca atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat
    29941 cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa
    30001 acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc
    30061 gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt
    30121 cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa
    30181 caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct
    30241 ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc
    30301 cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt
    30361 cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt
    30421 caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt
    30481 ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc ttatcatact
    30541 actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt
    30601 ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt
    30661 tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt
    30721 tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa
    30781 tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat
    30841 aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga
    30901 tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc
    30961 ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg
    31021 aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct
    31081 ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt
    31141 cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag
    31201 tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt
    31261 cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat
    31321 tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa
    31381 gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat
    31441 tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg
    31501 taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat
    31561 tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat
    31621 atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct
    31681 ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg
    31741 ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa
    31801 acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca
    31861 acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc
    31921 caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct
    31981 ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga
    32041 tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc
    32101 tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct
    32161 aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac
    32221 atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat
    32281 ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca
    32341 attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag
    32401 ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta
    32461 attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt
    32521 ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt
    32581 tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt
    32641 tcttcccaca tctgattgtc gggacgagaa tgattagcca gcaaaacaag atcatagttc
    32701 tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt
    32761 tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt
    32821 tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt
    32881 tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca
    32941 attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact
    33001 gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct
    33061 agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc
    33121 aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct
    33181 ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac
    33241 ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc
    33301 atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca
    33361 atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa
    33421 acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc
    33481 ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga
    33541 agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg
    33601 ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg
    33661 tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta atgcttcaac
    33721 aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc
    33781 aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa
    33841 agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt
    33901 actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg
    33961 caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg
    34021 tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc
    34081 agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc
    34141 caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt
    34201 caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc
    34261 gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt
    34321 aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt
    34381 cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat
    34441 gggcttggca aataaagctc tttatatgct tcttcaaaag gtttcgttac aaattcatcc
    34501 tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt
    34561 gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg
    34621 acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta
    34681 tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa
    34741 tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct
    34801 ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat
    34861 tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc
    34921 aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc
    34981 aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt
    35041 gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt
    35101 gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat
    35161 aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct
    35221 tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg
    35281 ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca
    35341 gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca
    35401 ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact
    35461 cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc
    35521 tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac
    35581 taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg
    35641 ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc
    35701 ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga
    35761 caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg
    35821 agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc
    35881 tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa
    35941 cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca
    36001 ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc
    36061 atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag
    36121 atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct
    36181 tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa
    36241 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg
    36301 cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga
    36361 acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca
    36421 gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag
    36481 gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag
    36541 ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt
    36601 cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat
    36661 caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat
    36721 agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc
    36781 cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa
    36841 tatgctgctt caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt
    36901 tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata
    36961 attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg
    37021 gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt
    37081 ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt
    37141 aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa
    37201 acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag
    37261 aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa
    37321 gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt
    37381 ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca
    37441 aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt
    37501 tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt
    37561 cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa
    37621 ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta
    37681 gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat
    37741 caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt
    37801 cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag
    37861 gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat
    37921 gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca
    37981 cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc
    38041 cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt
    38101 tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt
    38161 aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc
    38221 catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt
    38281 gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact
    38341 gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacagaact
    38401 gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa
    38461 tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac
    38521 tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat
    38581 acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat
    38641 ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc
    38701 accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata
    38761 tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc
    38821 agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc
    38881 tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa
    38941 agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac
    39001 ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa
    39061 atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaaat
    39121 aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg
    39181 caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg
    39241 aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat
    39301 gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc
    39361 gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa
    39421 atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa
    39481 taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac
    39541 gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt
    39601 tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa
    39661 tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc
    39721 gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc
    39781 ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc
    39841 tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt
    39901 gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt
    39961 ctccattcaa ttagtacccc ttctaatagc gaacgaaact gtacaatagt gtgtatggtt
    40021 tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata
    40081 gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc
    40141 aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt
    40201 ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga
    40261 atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt
    40321 tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca
    40381 agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag
    40441 ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa
    40501 aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg
    40561 tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg
    40621 ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa
    40681 attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg
    40741 ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct
    40801 cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac
    40861 aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc
    40921 tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc
    40981 ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt
    41041 ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg
    41101 aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata
    41161 attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa
    41221 aattgtactg atttgttgag gaaaccttgc gtgttggatc accgtttctg ttccaacaag
    41281 agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga
    41341 aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa
    41401 tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc
    41461 tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat
    41521 tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa
    41581 aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat
    41641 atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc
    41701 gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt
    41761 taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg
    41821 acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt
    41881 taccattaga ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa
    41941 agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt
    42001 cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc
    42061 aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt
    42121 ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa
    42181 ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta
    42241 gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga
    42301 aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac
    42361 gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat
    42421 ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat
    42481 gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa
    42541 atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa
    42601 tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta
    42661 gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa
    42721 gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat
    42781 tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg
    42841 tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc
    42901 ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc
    42961 gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt
    43021 cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta
    43081 actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag
    43141 aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact
    43201 gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc
    43261 cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat
    43321 tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt
    43381 gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa
    43441 aaaattgaat gctactcttc atttttaata tttatatata acaaatacat aagacgatcc
    43501 ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc caacacaaat
    43561 gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac
    43621 acaataaccc ttaaagcttc acgtggaata gtgaacgtct gcttttcact ggacatatta
    43681 caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc
    43741 gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg
    43801 atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg
    43861 atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt
    43921 tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc cttgattgat
    43981 tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc
    44041 tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct
    44101 ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca
    44161 ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga
    44221 ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat
    44281 acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc
    44341 tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct tttaggattg
    44401 gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt
    44461 ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta
    44521 cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc
    44581 ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta
    44641 tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt
    44701 ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt
    44761 ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg
    44821 gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata
    44881 cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat
    44941 tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg
    45001 tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa
    45061 ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag
    45121 atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg
    45181 gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc
    45241 tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg
    45301 aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac
    45361 aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa
    45421 aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca
    45481 caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca
    45541 tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
    45601 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
    45661 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
    45721 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
    45781 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
    45841 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
    45901 ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
    45961 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
    46021 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
    46081 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
    46141 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
    46201 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
    46261 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
    46321 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata
    46381 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
    46441 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
    46501 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
    46561 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca
    46621 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
    46681 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
    46741 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
    46801 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
    46861 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
    46921 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
    46981 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
    47041 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
    47101 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
    47161 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
    47221 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc
    47281 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
    47341 cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt
    47401 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
    47461 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
    47521 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
    47581 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
    47641 cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat
    47701 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
    47761 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaac
    47821 ggcaatgaat gccgct
//
LOCUS       DANKMN010000011        47044 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000011 DANKMN010000000
VERSION     DANKMN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(63..1079)
                     /locus_tag="PW224_000634"
     CDS             complement(63..1079)
                     /locus_tag="PW224_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2907346.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            1299..2387
                     /locus_tag="PW224_000635"
     CDS             1299..2387
                     /locus_tag="PW224_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL2907347.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            2441..3967
                     /locus_tag="PW224_000636"
     CDS             2441..3967
                     /locus_tag="PW224_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2907348.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            3969..5174
                     /locus_tag="PW224_000637"
     CDS             3969..5174
                     /locus_tag="PW224_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2907349.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            5431..6408
                     /gene="guaC"
                     /locus_tag="PW224_000638"
     CDS             5431..6408
                     /gene="guaC"
                     /locus_tag="PW224_000638"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL2907350.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            6672..8096
                     /locus_tag="PW224_000639"
     CDS             6672..8096
                     /locus_tag="PW224_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2907351.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            8557..9666
                     /gene="pdhA"
                     /locus_tag="PW224_000640"
     CDS             8557..9666
                     /gene="pdhA"
                     /locus_tag="PW224_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL2907352.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            9669..10646
                     /locus_tag="PW224_000641"
     CDS             9669..10646
                     /locus_tag="PW224_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL2907353.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            10671..12314
                     /locus_tag="PW224_000642"
     CDS             10671..12314
                     /locus_tag="PW224_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL2907354.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            12322..13728
                     /gene="lpdA"
                     /locus_tag="PW224_000643"
     CDS             12322..13728
                     /gene="lpdA"
                     /locus_tag="PW224_000643"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL2907355.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            13890..14165
                     /locus_tag="PW224_000644"
     CDS             13890..14165
                     /locus_tag="PW224_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL2907356.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            14171..14938
                     /locus_tag="PW224_000645"
     CDS             14171..14938
                     /locus_tag="PW224_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL2907357.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            15106..16935
                     /gene="typA"
                     /locus_tag="PW224_000646"
     CDS             15106..16935
                     /gene="typA"
                     /locus_tag="PW224_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL2907358.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            17196..18968
                     /gene="recQ"
                     /locus_tag="PW224_000647"
     CDS             17196..18968
                     /gene="recQ"
                     /locus_tag="PW224_000647"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL2907359.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            19082..19384
                     /locus_tag="PW224_000648"
     CDS             19082..19384
                     /locus_tag="PW224_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL2907360.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            19387..20550
                     /locus_tag="PW224_000649"
     CDS             19387..20550
                     /locus_tag="PW224_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2907361.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            20576..24004
                     /locus_tag="PW224_000650"
     CDS             20576..24004
                     /locus_tag="PW224_000650"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL2907362.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            24025..25149
                     /locus_tag="PW224_000651"
     CDS             24025..25149
                     /locus_tag="PW224_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907363.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            25139..25576
                     /locus_tag="PW224_000652"
     CDS             25139..25576
                     /locus_tag="PW224_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2907364.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            25560..25898
                     /locus_tag="PW224_000653"
     CDS             25560..25898
                     /locus_tag="PW224_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL2907365.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            26017..26577
                     /gene="rsmD"
                     /locus_tag="PW224_000654"
     CDS             26017..26577
                     /gene="rsmD"
                     /locus_tag="PW224_000654"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL2907366.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            26574..27065
                     /gene="coaD"
                     /locus_tag="PW224_000655"
     CDS             26574..27065
                     /gene="coaD"
                     /locus_tag="PW224_000655"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL2907367.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            27055..28098
                     /locus_tag="PW224_000656"
     CDS             27055..28098
                     /locus_tag="PW224_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL2907368.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            28257..28985
                     /locus_tag="PW224_000657"
     CDS             28257..28985
                     /locus_tag="PW224_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL2907369.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            29101..29346
                     /locus_tag="PW224_000658"
     CDS             29101..29346
                     /locus_tag="PW224_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2907370.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            29343..29561
                     /locus_tag="PW224_000659"
     CDS             29343..29561
                     /locus_tag="PW224_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL2907371.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            29697..30236
                     /locus_tag="PW224_000660"
     CDS             29697..30236
                     /locus_tag="PW224_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL2907372.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            30229..32502
                     /locus_tag="PW224_000661"
     CDS             30229..32502
                     /locus_tag="PW224_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL2907373.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            32540..33586
                     /gene="holA"
                     /locus_tag="PW224_000662"
     CDS             32540..33586
                     /gene="holA"
                     /locus_tag="PW224_000662"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL2907374.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(33675..33929)
                     /locus_tag="PW224_000663"
     CDS             complement(33675..33929)
                     /locus_tag="PW224_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL2907375.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(34329..34598)
                     /locus_tag="PW224_000664"
     CDS             complement(34329..34598)
                     /locus_tag="PW224_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907376.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(35068..35523)
                     /locus_tag="PW224_000665"
     CDS             complement(35068..35523)
                     /locus_tag="PW224_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL2907377.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(35661..37589)
                     /locus_tag="PW224_000666"
     CDS             complement(35661..37589)
                     /locus_tag="PW224_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2907378.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(37786..38619)
                     /locus_tag="PW224_000667"
     CDS             complement(37786..38619)
                     /locus_tag="PW224_000667"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL2907379.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(38811..39515)
                     /locus_tag="PW224_000668"
     CDS             complement(38811..39515)
                     /locus_tag="PW224_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2907380.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(39538..40164)
                     /locus_tag="PW224_000669"
     CDS             complement(39538..40164)
                     /locus_tag="PW224_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2907381.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            40490..42865
                     /locus_tag="PW224_000670"
     CDS             40490..42865
                     /locus_tag="PW224_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL2907382.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(42889..43380)
                     /locus_tag="PW224_000671"
     CDS             complement(42889..43380)
                     /locus_tag="PW224_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL2907383.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            43567..44487
                     /locus_tag="PW224_000672"
     CDS             43567..44487
                     /locus_tag="PW224_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2907384.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            44641..44985
                     /locus_tag="PW224_000673"
     CDS             44641..44985
                     /locus_tag="PW224_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL2907385.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(45175..45675)
                     /locus_tag="PW224_000674"
     CDS             complement(45175..45675)
                     /locus_tag="PW224_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2907386.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            46203..46286
                     /locus_tag="PW224_000675"
     tRNA            46203..46286
                     /locus_tag="PW224_000675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46237..46239,aa:Leu,seq:cag)
     gene            complement(46610..>47044)
                     /locus_tag="PW224_000676"
     CDS             complement(46610..>47044)
                     /locus_tag="PW224_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL2907387.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        15528 a         8169 c         9889 g        13458 t
ORIGIN      
        1 aaatttgtct tcgccaacac aatttgacga agagccaatt ttttgtcatg atacttctcc
       61 agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac agtctcggaa
      121 ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata acgagtaacg
      181 gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt gacgattcct
      241 gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat caaatcttct
      301 ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat ttcatcacta
      361 tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg tttcatctgg
      421 tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt catcggaggt
      481 ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga ttccaaaaat
      541 ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc taggattctg
      601 atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc ccgatttcct
      661 tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt cgtttgaata
      721 tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa tacagagtcc
      781 cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc aatgaacgtc
      841 cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag aacttggtaa
      901 tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca gctactctcc
      961 gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt attaggtgta
     1021 caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac acgtttcact
     1081 ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg aaaatcaaag
     1141 tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga caaaaaaagt
     1201 acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct ttctttatta
     1261 gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa tttagaaaga
     1321 ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt ggcaattcag
     1381 gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa tcagcagaaa
     1441 gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt tataaaacag
     1501 atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa aatatgatta
     1561 caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta actgatgtgt
     1621 tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta ctgatgaatt
     1681 cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta tcgcaagcag
     1741 cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg attgaattat
     1801 ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg atgtctgttt
     1861 tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg agtttcagtc
     1921 aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat aatctgttaa
     1981 gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat ccaataagtt
     2041 taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa cctttacctg
     2101 tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc ggggaacaaa
     2161 cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa atgatcaaag
     2221 caatcagtga tggagaagag gttccggtca atgatactga aacttatgat aatggggtga
     2281 aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat tatcaagcag
     2341 agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag gaaccaaaca
     2401 aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt gtattttaaa
     2461 aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg tgaatctgat
     2521 gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa aatcaacatt
     2581 gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa tcttttatga
     2641 tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca tcgtcatcat
     2701 tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt ttctcggaaa
     2761 tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa catatgaact
     2821 gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga ttggcgtagg
     2881 gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc tgttgatttt
     2941 ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga gattgatccg
     3001 agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga atgaaatcgt
     3061 ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga cactagaaaa
     3121 tgaaatgatc agcgaagagc atattattcg cggaatggtg ggcagagatt tagttgatcg
     3181 ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag actggacggt
     3241 ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc atttgaaaaa
     3301 aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat tcgctatgag
     3361 tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg aaggggcaga
     3421 gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg tctcggagga
     3481 ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga cgctgagcag
     3541 tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa ctgaggcaga
     3601 aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg tcagtagtct
     3661 tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac aacccgatat
     3721 tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg agatctacac
     3781 gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt ctgaacttcc
     3841 tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta ttacaggaga
     3901 agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga aagaagaggt
     3961 gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa ctaaatttga
     4021 aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta gcgggactac
     4081 ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat ataactaacc
     4141 ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta gttgtgctct
     4201 taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca attgcgggca
     4261 tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt cttgcagtag
     4321 gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata ccggcgttta
     4381 tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt ctaggaggcc
     4441 aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat ttaccagact
     4501 taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc atcacgatcg
     4561 tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta tttgaagtgg
     4621 aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta ctaggaataa
     4681 cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt ggtttgatcg
     4741 ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt tatgcgacgg
     4801 gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc acgttctggg
     4861 tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct gctcgattga
     4921 atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg gcagtatatt
     4981 ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt gtcggtggct
     5041 tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta gactggcagc
     5101 aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat aataagaaaa
     5161 aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa gacatttgtc
     5221 acagtctttt tttcgctaaa agcgaacatt cattgtttcg tttgtataga ctaaaaataa
     5281 tatatgtttt tttaccattc gtataaattc tctttttgct cttgattttt ttgcttaatt
     5341 gagttaaatt acatagggcg ctaaaaaaag ccttgattaa attaataatg ttcgtgtttt
     5401 gctggatgtg aacatcagga ggataatgta atgaaagtat ttgattacga agatattcaa
     5461 ttaattccta ataaatgtat cgtaaatagc cgttctgaat gtgatacaac ggtcacatta
     5521 ggtaaacata cattcaaaat gccagtagtg cctgccaaca tgcagacgat tattgatgaa
     5581 acaatcgcag aattcttagc ggaaaatggt tatttctata tcatgcatcg gtttgatgaa
     5641 gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg gattgattac ctcgatcagt
     5701 gttggcgtca agaaagagga atattccttt atagagaagt tggcagaaga atcgctaaat
     5761 cctgattata tcactatcga catcgcgcat ggacatgcta actcagttat tgatatgatt
     5821 cagcatatta aaaaatattt gcctgaaact tttgtgattg ccggtaatgt tggtacaccg
     5881 gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa caaaagtcgg gattggtcca
     5941 ggaaaagtat gtattacaaa aatcaagaca ggctttggga ctggaggctg gcaactggca
     6001 gctcttagat ggtgtgcgaa agcagctcgc aagccgatca tcgctgacgg tgggatcaga
     6061 acacatggcg atattgcaaa atctgtccgt tttggtgcga caatggtcat gattggttca
     6121 ttgtttgccg gtcacgagga atctcctggc gaaacaaaag tcgaaaatgg aatcgtgtat
     6181 aaagaatact ttggttctgc ttcagaattc caaaaaggcg aaaaacgaaa tgttgaagga
     6241 aagaaaatct ggatccaaca caaaggatct ttgaaagata cactagtgga aatgcaacaa
     6301 gaccttcaat catctatttc ttatgctgga ggaagagatc tggaagccat tcgaaaagtt
     6361 gactatgtca tcgtgaaaaa ttcgattttt aatggagaca caatctaaaa aataaataaa
     6421 atattaggat gttgtgaaaa agttgtcctg cattaagtaa taagaacagc caaacaaaaa
     6481 tagttcttta tatgttttgt ttggctggaa ttattatcgc agatacaagc ttttgaacat
     6541 cattattcag aataagggac tgtgataaga tttttgtcac agtcccttat gttttgcaaa
     6601 aaaagttttt cttttttctc gtaaattcgg taaaatcaaa agaaaaaata gacaatagaa
     6661 aagagaagct tatgtgggaa aaagtagaga aaaaacaagg accgatttat cgtcaaatta
     6721 tggaacagat tatgagagaa atcgaaaacg gaaggatcaa tcctggaagt caacttgaat
     6781 cagaacgaaa actagggcaa ctttttggtg tgaatcgttc caccgttgtc catgcattgg
     6841 aagagcttag ggagcttggg gtactaacga gcaaacgtgg cagcggatgg tttgttaatc
     6901 agactgaatg ggggaaattc gctgttccaa gaatcgattg gcggcaaatg atctctcctc
     6961 gttatgagca gacggactgg tatgagcaaa aaataagaga agcaaagcag atccaaaaaa
     7021 catcgtttct tgatttatac gctagcgaga tgccagatga gcttttgccg aattttgagt
     7081 ttccttctta ttctcttgaa gaaatcttag cggaagaaaa agaaatggat cttttaggct
     7141 atactccgtt acgtaacaaa atcaaacggt atttggaaag taaatttgaa ttggcatttt
     7201 cccccaacca gcttcttgtg actggtggcg gtcagcaagc aatctttttg attttacaaa
     7261 cgattctttc agctggagag gggatagctg tagagtcacc ttcttttttt taccgattac
     7321 ctcttttcaa agcttcagga acgcgtttat ttgggatacc gatggatgaa gaagggatcg
     7381 acttagaaaa actgaaagaa tcgatccata aaaataaatt gaaagcagtg cttatcaacc
     7441 cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa acgacgagaa aagctagtag
     7501 aattgtgtcg acagtatcgg cttccaatta ttgaagacga tgtatttacg gatctttcat
     7561 ttctaaacca agaagaagtc cttcctatac gaacgattga tccggataat gtgttatacg
     7621 tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat cggttgggtg ataggacctg
     7681 agacttttat ctcgcaacta gctcaagcgc aagaaatgat ggagttttcg atgaatatcc
     7741 ttacacaaat cacagtggct aatgtattta atgaagcaat tgatttgaaa atgcaagatg
     7801 taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc ttggggagat tcacaatcgt
     7861 ttttcaagct tcattcaatt ttaggtggtt actatgcctg gattacttgg gatgggaaaa
     7921 aactagaccc cgaactagca gaaaaagtag tagatatggg tcttggaatt gcaccaagtc
     7981 tactttttgg gcaagatata aatggaatca gaatcaatta cagtaggctg gatgaaaacc
     8041 aaataccgtt gttttattca aagatgaaaa ttctagaaaa gtggttaaca gaataatttc
     8101 acaaagtaaa tagattaaag aatataacag cttggattta aatttcaagc tgtttctttt
     8161 ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa accattattt gtacgtccgt
     8221 tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca acgtttttaa
     8281 atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat gaaatataaa
     8341 acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt ggcataattt
     8401 cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca tgataattta
     8461 ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg cagaatgaag
     8521 agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa agcaccgatc
     8581 gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca agcacttgat
     8641 caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga tgaactcgtt
     8701 gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac agcgctgaac
     8761 cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag tcagctggct
     8821 agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga tgtacctcaa
     8881 ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg acatgctgca
     8941 ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat cggtgcacaa
     9001 tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa aaatgtcgtc
     9061 ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc aatcaatttc
     9121 gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta tgcgatctct
     9181 acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt cgcagctggg
     9241 attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt atctaaaatg
     9301 gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact gacttatcgt
     9361 tatggaccac atacactatc tggtgacgat ccaacccgct accgtacaaa agacacagat
     9421 gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac ggaaaaagga
     9481 ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga aatcaaagca
     9541 gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt gaaaaacatg
     9601 ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc gaaggagtcg
     9661 aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta gcacttgaac
     9721 tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac ggaggagttt
     9781 tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc gatactcctt
     9841 tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga tttcgtccag
     9901 ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta gtagctcaaa
     9961 tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca attcgttcgc
    10021 catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa ggtttgatcg
    10081 ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca aaaggactat
    10141 tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg aaactatatc
    10201 gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat aaagcagcag
    10261 ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg cgtgaagcaa
    10321 tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt gatcttcgta
    10381 cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact ggtcgcgtag
    10441 ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt tctgagatct
    10501 cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca cctgatacag
    10561 tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat atcgaagaca
    10621 aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc atggcttatc
    10681 aatttaaatt gccggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc aaatggttcg
    10741 tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa aatgacaaat
    10801 cagtagaaga aatcccgtca cctgttacag gaacggttaa aaatgtgatt gttccagaag
    10861 gtaccgtagc aaatgttggg gatgtattag tagaaatcga tgcaccagga cacgaagaca
    10921 atgaagggga cagtggtgta gccgcagaat cccaaactcc agctaaaccg gctgctgaac
    10981 ccactgttga tacagaatca gctggatcgt caagcgaagg agtcttccaa tttaagttac
    11041 cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag
    11101 atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca gtagaagaaa
    11161 ttccgtcacc tgtcacaggg acagttaaaa acgtgattgt tccagaaggc actgtagcaa
    11221 atgttgggga tgtattagta gaaatcgatg ctcctggaca caatagtgca ccgtcaacta
    11281 gtgcgccatc agctgaagca ccaaaagaaa aagtggaaac atcaggttca gcaagtgtcg
    11341 ttgaagctgc cgatccaaat aaacgtgtat tggctatgcc atctgttcgt caatttgccc
    11401 gtgaaaaaga tgtagatatc tcacaagtaa ctgcaactgg aaaaggtggc agagtaacaa
    11461 aagaggacat tgaaaacttc ttggcaggtg gtccaagcag tgcaccagcg aaatcagaag
    11521 cgccagaagc ggcagcaccg aaagaagcag ctcctgcagc agaaagcaaa ccagctgcac
    11581 cagcaaaacc attcaagtcc aacttaggag acttggaaga acgtgtagca atgacgccaa
    11641 cacgtaaagc tatcgctaaa gcaatggtca acagtaagca tacagctcca cacgtgacat
    11701 tgcatgatga agtagaagtg tctaaactat gggataatcg gaaacgattt aaagaagttg
    11761 ctgctgcaaa tggtacgaaa cttactttct tgccatatgt cgtgaaagct ttaacagcga
    11821 ctgttaagaa atacccagta ttgaatgcat ctatcgacga tgcaaaccaa gaaatcgttt
    11881 ataaacatta ctacaatatc ggtattgcaa ctgatacgga tcacggacta tatgtaccaa
    11941 atgtgaaaga tgcagaccgc aaaggaatgt ttgctattgc agacgaaatc aatgagaaag
    12001 caaaattagc tcatgacggc aaactatctg cagaagatat gcgcaatgga acaattacga
    12061 tcagcaatat cggatctgta ggtggcggtt ggtttacacc agtcatcaac tatccagaag
    12121 tagctatttt aggtgtagga acgattgcac aacaaccaat cgtaaatgct gaaggcgaaa
    12181 tcgttgtcgg tcgtgtgatg aaactgtcat taagtttcga tcaccgtatc gtagatggag
    12241 caactgcgca acaagcaatg aataatatca aacgtctttt agctgatcca gagctattaa
    12301 tgatggaagg atgataaaca aatggtagtt ggagattttg ctgtagaatt agatacagtt
    12361 gtaattggtg cgggacctgg tggctatgta gctgccatcc gcgctgctga aatgggacaa
    12421 aaagtagcaa tcattgaaag agagtatatt ggcggtgttt gtttgaatgt cggctgtatt
    12481 ccttcaaaag cactgatagc tgcaggacac cattatcaag agtcactaga ttctgaaatg
    12541 tttggagtaa ctagtgaaaa cgtcaaacta gattttgcaa aaacacaaga atggaaagaa
    12601 aacaaagttg tccatacgtt gacttctggt gttggtttcc tattaaaaaa acataaggtt
    12661 gaaacaatcg aaggagaagc gttcttcgta gatgatcaca cactacgcgt cattcatcca
    12721 gattcagctc aaacttattc gttcaataac gcaatcatcg caacaggttc ccgtccaatc
    12781 gagattccag gatttaaatt cggaggacgt gtgttagatt ctactggcgg attagcattg
    12841 aaagaagtac ctaagaaatt tgtgatcatc ggtggcggtg tgattggtgc cgaattgggc
    12901 ggagcgtacg caaacttagg agcagaagta acgattcttg aaggctcacc acaaatttta
    12961 ccaacttacg aaaaagatct agtaaaatta gtagaagatg atttcaagaa aaaaggcgta
    13021 acagtcgtaa cgaatgcaat ggcgaaagaa gcagtcgata acggtgacag cgtgacagtg
    13081 aaatatgcag tggacgggaa agaagaatcc gttactgctg attatgtgat ggtcactgta
    13141 ggtcgtcgtc cgaatacaga tgatatgggc ttagaacaag cgggtgtcga agtaggagaa
    13201 cgaggattga tcacggtgga taaacaagga cgtacgaacg tacctaacat ctatgccatc
    13261 ggtgatatcg ttccaggggc ggcattagca cacaaagcca gttatgaagc aaaaatcgct
    13321 gctgaagcaa tctctggcaa aaaagtagct gtagattata aagcaatgcc ggcagtagcc
    13381 ttcactgatc ctgaattggc ttctgtaggt atgacgatca aagaagcaaa agatgctggt
    13441 atcgaagcaa aagcatataa attcccattt tcagggaacg gtcgggcatt atcgcttggt
    13501 aaaacagaag gattcatccg tctagttacg acgatcgaag ataacgtgct tattggcgcg
    13561 caaatcggtg gagtcggcgc aagtgatatg gtctctgaat tagctttagc aatcgaatca
    13621 ggaatgaatg ctgaagatat cgccttgacg attcatcctc atccatctct tggcgaaatc
    13681 gtcatggatg cttcggaatt agcattaggt ttaccaatcc atatttgatt tgaataatac
    13741 aggcgaaacg caactgaaaa atcagttgcg ttttgttttt tagaattatc aataatatta
    13801 ttatgtaaac ttaaaaatat gacaacttct tggtttatta ccacttccta tgctacactg
    13861 aagaggaata tgaaatagga gagttgaaga tgcaagaata tcagtatcca cttgatttag
    13921 attggacaac agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg gaacgtgcga
    13981 atgaaaaagg gataaacaat gaagcttttt tgaagactta tcagcagttt aaaactgttg
    14041 tcaaatccat tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct tccggttatt
    14101 ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa caaaactttg agaatgaacg
    14161 ggtgagataa atggaacaga tgattgatca tatcaagtcc tggcttgaag aagctggtga
    14221 gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa aaatcaaatc gaacggatct
    14281 agtaacaaat gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc attcctttga
    14341 tccagaggcg aaaatattgg cagaagaaga agggtgtaat caattatcgg atctcgatgg
    14401 aagagtcttt atcatcgatc ctattgatgg caccttgaat tttgtgattg aaggggaaaa
    14461 tttttgtatt atgctcgctg tttatgaatc cggcgttgga aagcttggtt tcatttatga
    14521 cgtgatgaga aatgaacttt tttggggtgg aaaaggaata ggcgtgtata aaaatgatga
    14581 gccattgcct agaccagcaa ataaatcctt atcggaaggc ttacttggtg tgaatagtta
    14641 tcttttcggt cataataaat atggaatccg agaaattgcg gaaagcagca tggggacacg
    14701 tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg aaaggaaacc acattgctta
    14761 tatttcaaac cttagccctt gggattatgc agcgggaaat gtcttattgg aagagtttgg
    14821 tatggtaaca agcggtcttt ttggtactcc tttgacattt gaaaaaaggg aatactattt
    14881 agcagggact tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag aaggctgaaa
    14941 aaaaggtaga aatcattcac aaaaatgata aaaacgagca aaagattaat tttctcgaaa
    15001 aaatgaaaac attttactat atttcatgaa aaaagtctgc tataattagc agtcgagtaa
    15061 aatttgccct gtcataaaga accaataaaa aaggagttag ttacattgaa ttatagaaac
    15121 gatatccgta acgtcgcaat tattgcccac gttgaccacg gaaaaacaac attagttgat
    15181 gaactattaa aacaatcaga tacattagac gcacatacgc aattgcaaga acgtgcgatg
    15241 gactcaaatg cgctagaaaa agaacgtggt atcacgattt tagccaaaaa cacagctgta
    15301 gactataaag gtattcgcgt aaacatcatg gatactccag gacacgcgga cttcggtggc
    15361 gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga tgcgtatgaa
    15421 ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca tatcacacca
    15481 atcgttgtcg taaacaagat tgacaaacct tctgctcgtc ctgagcatgt cgtagatgaa
    15541 gttcttgaac tattcatcga attaggagca gatgatgatc agttagactt tcctgtgatt
    15601 tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg atccagcaga tcaagaacca
    15661 accatggcac ctatttttga tacaattatt gaaaaaatcc ctgctcctgt cgataacagc
    15721 gacgaaccat tacaattcca agtttcatta cttgactaca acgattatgt aggtcgtatc
    15781 ggaatcggac gtgtatttag aggaacaatc aaagtaggag accaagtagc attaattaaa
    15841 ttagacggaa cagttaagaa attccgtgta acgaaattat ttggtttctt tggactaaaa
    15901 cgtctagaga ttcaagaagc aaaagctgga gacttgattg ctgtctcagg tatggaagat
    15961 atctttgtcg gtgaaacagt tactcctgtg gatcatcaag atgcgttacc tatccttcac
    16021 attgacgaac caacattgca aatgactttc ttagttaata attcaccatt tgccggtcga
    16081 gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga acttcaaaca
    16141 gatgtatcct tacgagttga accaacaaac tctccagatg catggacagt ttctggtcgt
    16201 ggagaattgc atttgtctat cttgattgaa aacatgcgcc gtgagggata cgaattgcaa
    16261 gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg taaaatgcga accgtttgaa
    16321 cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg ttatcgagtc cttgagctta
    16381 cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg gacaaatccg tttgacattc
    16441 ttaacacctg cccgcggttt gattggttat tcaactgaat tcctttcaat gacacgtggt
    16501 tacggaatca tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg tcaaattggc
    16561 ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa aagcaacgac ttattctatc
    16621 atgagtatcg aagaacgcgg tacagtcttc gtagaaccag gtacagaagt atatgaagga
    16681 atgattatcg gggaaaacag ccgtgataat gatttgactg tcaacatcac gaaagcaaaa
    16741 caaatgacaa acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa aaaaccaaaa
    16801 caattgacgt tggaagaatc tttggaattc ttgaatgatg atgaatattg cgaagtaaca
    16861 ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga aaaagcatct
    16921 aaaaagaaaa aataatctgt aaaaggatga agagagaaac cagcttgtta taaaaaatag
    16981 ctggtttctt tttttgtcca actcttttct gtggagaagt catctttgtc aatggacata
    17041 aattcagaaa atacgccgaa agacgtttaa aagactttta aaaaacttat ttctagagag
    17101 tttttgtttc ataataagat aatgtttcac gaaaggagat caacttgtcc ttcgaagaaa
    17161 atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa cgacttatta aaacaatact
    17221 ttggctatga tgagtttaga cctggtcaaa aagaaattat ccagaaagtc attgatcagg
    17281 aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc aatctgttac cagcttccag
    17341 ctttactatt agatgggctt actgtggttg tgtccccttt aatctctttg atgaaggatc
    17401 aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt tatcaatagc tcgttagatg
    17461 gttacgaaac agctagacgc ttccaagaaa tcgatagaca gcagtaccgt ttactttata
    17521 tcgctcctga acgttttatc atgccggatt ttatccaagc aatgaagcga tggaacgtcc
    17581 gtatgatcgc tattgacgag gcacattgta tctctcagtg ggggcatgat ttccgcccaa
    17641 gttatttaca gatggcgaac cagctagatc aactgccaaa tcgtcctgta attgttgctt
    17701 tgacagctac agcgactgtg caagtagctg ctgacataaa aagactttta aaaattccag
    17761 aaaacaacca tattcagact ggttttgaac gagaaaatct acgatttcaa gtaattaaag
    17821 atcaaaaaaa agaacaatat ctaatcgaat atttaaagat caacaaaaat caatctggta
    17881 tcatttatgc agcaacgaga aaagaagtag accgtctcta tcatttgctt aaaaaattcg
    17941 atttttccgt gggtagatat catggtggtc taaatgagaa tgagcgaaca gaaatgcaag
    18001 aagccttttt atacgatcgc ttgcagctga ttgtagcgac aaatgcattt ggtatgggta
    18061 taaataaaag caatgtccgt tttgttatcc attatcaaat tcctggctct ctagaagcat
    18121 actatcagga agctggtcgg gcgggaagag atggtctttc cagtgaagcc atcctgcttt
    18181 tctctcctca agatatccaa gtccaaaagt tttttatcca gcagtcccaa agagaagaag
    18241 ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac agaatacgtg tatattgagt
    18301 cctgtcttca gcaatacatc ctaaactatt ttggcgagac aagcagtccg tgcaaccgtt
    18361 gtggtaactg tttagatgac agagaaatag tagaagtaac gacagaagca cagatggttc
    18421 tttcttgctt aaaacgtatg ggtgagaact atggtaaaca aatgctgatg aaagtcttag
    18481 caggatcgaa agaacaaaag ctgcaagctt taggttttgg acatttatct acctatggac
    18541 tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat tgaatatttg atatcgaatg
    18601 gctatcttct gaccatcaat ggtgagtatc ctgttttgaa agtaacagaa agagggatac
    18661 aagtattgaa aggacaagaa tctgtttatc gaaaggaacc caagaaagtc cagcagctat
    18721 cggatgaaga aaccgataca ctatttgaag tgctgcggga actgcgaacg gatctagcat
    18781 cagaagcagg cgttcccccg tatgtcgtat tttcagattc cacattgaaa gaaatgagcc
    18841 gaatacgtcc atcaagcaga ttggagatgc ttcagataaa aggagttggc caaagcaagc
    18901 ttgataaata tggcgaagcc tttttatctc ggataaaaaa tgcggactct aacgttctgg
    18961 ataagtagaa agaacgaata cgtaaaaatg aataaaaaca actggttaaa tctgtttttt
    19021 ttgctcctta tgaaaatcaa atatgttata attaaggaat tagaatggga ggtaagcaca
    19081 gatggattct ttgtcgaaaa aacttgcgat ccaagtatta aatgatgaag cagaacggat
    19141 caaacgatta atccgaaatc aaaacaacag tctctgtatc tcacaatgca aagccttcga
    19201 agaagtggta gatacacaga tgtacggctt ttcccggcaa gtcggttttg ctgtccgtat
    19261 aggagtaatc ggaaagaacg agggccagct gatgttaagt gagctagaac gagaattgaa
    19321 tcagttatat agtgaggttt accaagaaat ttatgataaa aaagaagttg gcaaggagga
    19381 atagacttgc ctaagaaagt gaaaaaaagg catctattag actatagcat cttgattccc
    19441 tatttaattt tatgtgtatt gggattgatc atggtatata gttctacgtc gtatctatta
    19501 ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc agagtatttt ttgggtgctt
    19561 agcttgattg ctatcgcact actttataaa atgaaaacag atgttttgaa aaaccagcgt
    19621 ttgatcatgg cagccatcgc agttttgacg attttgctgc tgattgttgt gttttttggt
    19681 aaggagatca atggggcgaa aggttggctg caaattgcag gattctcgat ccaacctgcc
    19741 gaatacctaa aaatcatttc tatctggtac ctgtctctga ctttatctaa aagacaaaac
    19801 agtgtacaaa aagattttct tgggactgtg aaacgtccac ttgcaatggt tatcggactt
    19861 actgcattag tcgctattct tcccgatttt ggtaatgcgg cagttatctt tttgattatt
    19921 ttggttctgc ttttagccag cggagtcaat tatgtgtata ctttgattgt aggagtagga
    19981 ggcttttgtc taagtacttt tacgatctgg ctgatcaata tcacaaacgg aaagatctta
    20041 cccggacgtt tacagtatat ctataatcga tttgctattt accaaaatcc tttctcagac
    20101 gaattaaata aagggcatca actagtcaac ggctactatg ccatgttcaa cggaggacta
    20161 tttggcaggg gattaggtaa cagtatccag aaaaaaggat ttttacaaga agcacaaacc
    20221 gattttattt atgcaatcgt agtggaagaa ctgggagtga ttatggggat tttgatatta
    20281 gcgttgctat tctttatgat tgtgcgcatt gtccttgtag gtatccgttc aaaagatccg
    20341 ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt tgatccaagt atttgtcaat
    20401 cttggaggaa ttactggcgt tattcctttg acagggatca cgttcccatt tttaagtcaa
    20461 ggaggctcca gtttgctgat gctttcgatt tgtgtcggct ttgtcctcaa catcagtgcg
    20521 gatgaaaaac gcaagagctt aggtttataa taaaaaaaga tgtaggagtg gttcgatgaa
    20581 aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt gtatttcgtg cttgtacaga
    20641 attaggaatt aaaactgttg gaatctatgc agaagaagat gaatattccg tacaccgttt
    20701 caaagcggat gaagcatatt tagtaggaca aggaaaaaaa ccgattgatg cgtatctaga
    20761 tattgaaggg atcatttcta tcgcgaaaga atgcggagct gacgctattc atccgggtta
    20821 cggtcttttg tcggaaaact tgaattttgc gcaacggtgc gaagaagaag gcatcatttt
    20881 tgttggaccc aaactgcatc atttagatat ttttggagat aaaatcaaag caaaagctgc
    20941 agctatcgaa gcggggattg cttcgattcc aggcacagat ggaccaatcg cttctataga
    21001 cgatgccttg gagtttgcga aacaatacgg ttatccgatt atgatcaagg cagctctagg
    21061 cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag agtgcaagag agggctacga
    21121 acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat gaagtgtatg ttgaaaaata
    21181 catcgctaat cctaagcata tcgaagtaca gattctcggg gatacgcatg gaaacgtcat
    21241 ccatcttttt gaaagagatt gttctgttca gcgccgtcac caaaaagtag tagaagtagc
    21301 gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc tgccaggcag ctgtccaatt
    21361 gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa tttcttgttg aaggagatga
    21421 tttttacttt atcgaagtca accctcgcgt ccaagtggaa catacgatca cagaaatgat
    21481 tacagatatt gatatcgtaa caacgcaact gttgattgct caaggtttgg atctgcataa
    21541 agaaatcgga ttgcctcagc aagaaggaat taaattaaat ggttcagcca ttcaatgccg
    21601 aatcactaca gaagacccgt tgaataactt tttaccagac acagggaaaa tcgatacgta
    21661 tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga aatgcttacg cgggctatgt
    21721 agtgacaccg tattttgatt cattgctagt aaaagtatgt acgcatggtg ctacctttga
    21781 aacagccatt cagaaaatgg aacgttgctt gaaagaattt agaatccgtg gagtgaaaac
    21841 aaacattcca tttatgctca atgtcatcac acatcctgaa ttccaatcag gaaatgcaaa
    21901 aacaaccttc attgatagta cagcaacatt atttgaattt cctcgactac gagaccgcgg
    21961 aaacaaaacg atgaaatata tcggtgaaat tactgtgaat ggttttccag gtatcgaaag
    22021 tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa gatctaatca caagaactga
    22081 ttacgtaacg gctaagaatg tattagatgc gaagggtgcc gatgctttgg tcgaatggat
    22141 caaaggacag gaaaatttgt tgttgacgga tacgacattt agagacgccc atcaaagtct
    22201 gctagcaact cgtgttcgga caaaggattt caagcagatt gcacgtttga ctggagaagg
    22261 tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca acatttgatg tggcttaccg
    22321 ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt cgttctttaa tgcctaatac
    22381 gttgctgcaa atgcttttca gaggttcaaa tgccgttggg tattctaatt atcctgacaa
    22441 tgtattggtg gaatttgtca aagaagcagc agcgcaagga atcgatgtat ttcgaatctt
    22501 tgatagtttg aactggactc cacaaatgga aaaaagtatc caagctgtac gggatactgg
    22561 taaaatcgca gaggcagcta tttgctatac tggcgatatc aacgatccaa gtcgggcaaa
    22621 atacaatgtt cagtattata aagacatggc aaaggaacta gaacaactgg gtgcgcatat
    22681 cattgcaatc aaagatatgg ctgggctgct taaaccgcag gcagcttatc gtttgattag
    22741 tgaattgaaa gaaacgacag atctgccaat ccatctgcat actcatgata ccagcgggaa
    22801 tggaatcatc acttattctg ctgcttcaaa agcaggagta gacatcgttg acgtggcaat
    22861 gagtgcaatg agtgggaata ctagtcagcc tagcatgagc agcttgtatt atgcactggt
    22921 caatggtcca cggctgccag aaatcacgat tgaaaatgca cagaaattaa accattattg
    22981 ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc ttgaatgctc ctgaaacaga
    23041 agtatatatg catgaaatgc caggaggaca gtattcgaat ctacagcaac aggcaaaagc
    23101 tgtcggatta ggtcatcgct gggatgaaat caaacaaatg tatcatactg tcaacttgat
    23161 gtttggcgat atcgttaaag taactccatc atctaaagta gttggagata tggcactatt
    23221 tatggtccaa aatgatctta cagaagaaga tatttatgaa aaaggcgaaa cactaagttt
    23281 ccctgaatct gttgtgactt tcttccaagg agaactaggg caacccgttg gcggatttcc
    23341 taaaaaattg caaaagatta ttcttaaagg cagacctgca ttgaatgaac gtccaggtct
    23401 tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa ttggcagaaa aaattggcta
    23461 tgaaccaaaa caagacgaag tattaagcta tttgatgtat ccgcaagtct ttttggacta
    23521 tcaaaaagca tataatcaat ttggcgatgt tacattactg gacacaccaa catttttcca
    23581 aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa cgcggaaaaa cgcttatcat
    23641 tcgtttagat gaaatcggag aacccgatat cgaagggaat cgcgtactgt tcttcaattt
    23701 gaatggacaa agacgtgaaa tcactatcaa agataattcg atcatcagta cggtccaaac
    23761 ccgattgaaa gctgaaccga caaaccgaga acaaattgga gcaacgatgt ctggttctgt
    23821 tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa ggtgatacgt tgatgattac
    23881 tgaagcgatg aaaatggaaa cggccatcga agcacgtttt gacggagaag tcgcacatgt
    23941 atatgtgtct tcaggagaca ctatttcgtc tggtgacctt ttgattgaag tgactgagaa
    24001 ataataagcg aaaggtgggg aaacatgaaa agactgggtt tgttttggct gattctgcta
    24061 ttagttattg tggcattcta catggaacca gtcttgtttc cgccaaaact tgaagtgaag
    24121 gaaaactctg atcaacaaat atcaaaccaa atgacaacgt ggaaatacca ggagcttaaa
    24181 gcaactggtt tttctactta tatcggaaaa ccgatcgatc agctggaaaa ggattttggc
    24241 caagcctatg atcgattaac tagcggattt ggttttgaaa caagatatta tgaaaataaa
    24301 gctagtcatc tcacatttga agcgaacatc caagacagca gagtcacaac tgttaaagta
    24361 ctgcaaacag ggcctgctga tatcgcgccg ttttatttag ggatgacgat gcaagatctt
    24421 acgaaaatca ctactatcta tactaatttc acttttgagt accatgatac agaagttgga
    24481 atcgaactaa tggaagagga tatgaattat cgtccgctaa ttgcttttga taacgacact
    24541 tttgctattt tattttttga tcaaacaaca aacggcctat tttcagttgt gtatttagat
    24601 aaagatagtc ttttgaaact attgccatat caagtgtttg gtgaaggcct tccacattat
    24661 caagcagata aatcggctga ttgggaatca atcaatcgcg ttaaagaacg aaaaagtacg
    24721 actttattaa atatgcttag aagagaagac gaacttccca tatacaatca aacacttgcc
    24781 tttcaaaacc acagtcagct gctactacat gattttttga caaggcctga ggaaatattg
    24841 tcagaggaac gtatggctga ttggcaaaaa tcgctaggca gtgctcaaac gacagtgaaa
    24901 ttctcactga cgaatgatga attggagaag ttaatcagta aacaaaaatt agaaaatact
    24961 agtgggctat ttattcatcc ggcgattgac gctacttttt cgtttttata ttggtacagc
    25021 gatccttaca accatgaccg tttcatggac ccgggaacgg attctgtagg cattgctttt
    25081 tctaaagaaa atatgttagt cttattgcaa gaagaaaacg gagaaagtag tgatagcagt
    25141 gatcgttaac gaagagctgt ttcaactgga ggatcaatgc ctccaagtcg ctgaaatgat
    25201 aaaaaagagt aaaacgatgg cttcttatct tgaacataaa cagtcaatgg aagaatctgc
    25261 aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa gaagcatttg aaaatgtagc
    25321 ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa cgagctgttc gaaaagcaaa
    25381 aagacagctt gatatggatg agcatgttgc agctttccga tatagtgaaa cacagctgca
    25441 aagtctgttg gatcaaattg tccaagtact tgccgaagaa gtttctaatg aaatcaaagt
    25501 agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt ggatgcggag gtaattgtca
    25561 tgcaattaga caatagtgaa tttgaaataa caaaacgacg cgggctgatc gtatgggtct
    25621 acagcttaaa acaattaaag aatctgaaaa agttcggctt gttgcattat gtctcaagaa
    25681 gaatgaaata tgtcgtgctt tacttaaatg aagagacatt ggaagaaaca gaagaacgaa
    25741 tccgtaaatt acattttgtt cgaaacgtag aacgttctta ccgaccggat atcgaaatga
    25801 attttgccga aaaaatagga aacaaaacag tattaaaaga cgaaggtttc gaaattgaag
    25861 agctaaatac agaaatccga ttagctgaac atctttaaaa atctcttatg agcagttttt
    25921 aaataaaaaa taacttggat aacgatcgat ttttgatcat tgtccaagta tttttttggt
    25981 atactagtac cagtaccatt tctaaggagt gagtagatgc ggataatatc aggaaattat
    26041 ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca gaccaactac agataaagta
    26101 aaagaatcca tattcaatat gattggacca tattttgatg gagagacagt attggatctg
    26161 tttgcaggaa gcggtggttt agctatcgaa gcagtatcta gaggctgcag tcatgcggta
    26221 tgcgtagaca aaaattacca agcgctaaaa atcatcaaag aaaacatcga aattacgaaa
    26281 gaaccagaga aatttaccac aataaaactt gatgcggaca aagcaatcga acggctagca
    26341 gcggagaaac aacaattcga ctacttgttt cttgatccgc catatgcaaa acaaaaaata
    26401 atcgatcaaa tccataaaat ggaagaactg ggactttttt ctaaaaccgc agttatcgta
    26461 tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg ctggattcca tcaaataaga
    26521 aaacagattt atggcataac ggccgtaact atctatagaa aagagggaaa tcaatgaaac
    26581 gtgcattatt cccaggaagt tttgatcctt ttacaaaagg tcacttagat acggtggaac
    26641 gtgcggctaa attattcgat gaggtagtaa ttggtgtttt tatcaatact tcaaagaaaa
    26701 gtttgtttcc cccagaagaa agaatgacat tgatcacaaa agctgtttct catcttccta
    26761 atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga aacagcaaaa gagattggtg
    26821 cagatgcgtt gatcagaggc atacgaagta tcaaagactt tgaatatgaa cgagagattg
    26881 ctcagatgaa tcatcatcta tatgatgaat tggaaactgt cttcttatta gcaaaaccag
    26941 aatatagtca cgtgagttcc agtattttaa aagaagtact gcactttggc ggagatgtga
    27001 gcagctactt accgccagtg atcaacgaag ctttagctgg aaagcgtgaa tcaaatgaaa
    27061 gctaaacaat ggttgaaaac agtactgatt atcatatttg gaatgttgat cattggattt
    27121 gtagtagttc ccttgccgta ttatgtagaa tcaccgggag ccacgatcga tctgaaagaa
    27181 ctaattacgg tcaatgataa ggaagataaa ggttctgggt ctttctctct tacctctgtt
    27241 ggtatcaggc aagctacagg tttcttagct ttaaaagcta aaatgacccc ttttgaagaa
    27301 ttgatcagtg aagaagaatt gactggtggg gcgacaaatg aagaatataa tcaaatgcaa
    27361 cagtattata tggattcttc ccaaaatgca gcaatcgaac aagcgttaaa acttgcagac
    27421 aagccttata aaatggaatt caaaggcgta tatgtcatga gtgtagagaa aacatcgaat
    27481 ttctttggca agctttcagt tggagataca gtaacaaaag tagacgggaa aagctttcaa
    27541 agtacagaag catttatgga ttatgtaaaa agccaaaaag ttggacaaac aatcgaaatc
    27601 agttatttgc ataatggtga agaaaaaaca gcttctggtg atctgatcga gttaccaaca
    27661 gataaaaagg caggaatagg tatcacgctt actgaccata ccgagatcga atcagataca
    27721 gatgttcgta ttaattctgg ctctatcggt gggccatcag cgggattgat gttcacttta
    27781 gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg gtaagcaaat tgcaggaact
    27841 ggaacgatca acagtgaagg agaagttggc cgaatcggag gaatcgataa aaaagtggca
    27901 agtgcggata aagctggcgt agagattttc tttgcaccag atgatacgat ttcagacgat
    27961 gttaagaaag aatatcctga aataaaaacc aactacgaag aagcaaaagc tgccgctaaa
    28021 aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag tacaagaagc gcttgactat
    28081 ctagagaaaa tgaaatgaaa ctaaataatt aaagaggggt tgtgacacga gttctgtcac
    28141 aacccctctt tttcgtcaaa aatcttcacg ataaaaataa aaacaaaaga aaaaattgac
    28201 gtaattagat gttgaaggga cgattttgcc agagctcact gtttcttcaa ttttaaatga
    28261 aaggagtaat gaatgatttt tactggtcat tctttgaaaa aagaacaatg atagaaaaaa
    28321 tcagcaagaa aatactagct tcacccaaat tgatcatttg gatgtctatg atagttctta
    28381 tcttttgtat cgcaagtggt tttttattat caaatttgct attttataga gaaacagaag
    28441 agactgctgc aaaagaaata ctttcttcgt ttagttccag tggagaagat ccatcagctg
    28501 aaggaaacga tgtagaagag gatgctcgtc caaaggtcat gtacaccgat atcaaaggaa
    28561 gtgtgaagga gcctggtatc tattcctttt cttcagaaga acgggtctat gatgtactaa
    28621 agcgtgcagg tggattatta gaagaagcgg atagcgatcg aatcaacttt tctgcgaaga
    28681 tagaagatca gcaagtccta tatatccctg ctgtaggtga agagctgcca gaacatcttg
    28741 atcagttggc atcctcggaa ggaaaacagc ctacagcaga ttccgagcct cccaaaataa
    28801 atatcaatac agccactcca tcagaacttc agcagattcc tgggatcgga tcagtcaaag
    28861 cgcaagaaat cattcgattt agagaagaaa acggatcctt ccagaaagtg gaagacctgc
    28921 aggagatttc tggtatcggt gaaaagacag ttgaaaaact aaaaaacttc gttacaatta
    28981 aatagaatga ttcaaaatcg actggcacga gcaataaatg gacgctaatg gatactaaga
    29041 ataatccaat tctaagagct tggcaggacg aggaggtagt atttttggat aggaaatagc
    29101 ttgattgttc gcaaagaaac attaaaaaaa ccaatgctga atgtctatct ccaaaataaa
    29161 atatctggga ttcatattat gaatacggct gtcagcggca ataacagtca agctctacga
    29221 gaacgctttg ccaaagatgt acttagctat acagcagata aagtatttat tctcattggg
    29281 acgaatgatc tagcagagca taaacaactt tctaaagaaa cgtaccaaaa aatctgcagt
    29341 ggatgatagt tcagctgaaa aaagactata gttctgatca tgctttcttt atcacgccac
    29401 cagcagtcga tgagacaaaa caaaaatatc ggaataacaa gttgatcaaa gaatatatct
    29461 ctgttctcac agaagtagca aaacgccatg attgtgaagt atcgattttt atacggaatt
    29521 tcttgaacaa atggattcga agaattttta taatgggcta atggatgacg gacttcattt
    29581 cggcagtgaa gggtaggatc ttttgtcaga caggatgatt cgttatattt aaagaatatt
    29641 ttgtttaagc aatgagtact ttttaatcaa cttgaaaaaa cggaggaata taaaacatgt
    29701 tagaaaaatc ttcaaaagca tgcgatacga ataaagaaaa agttactgat aagtcacata
    29761 atgaacgcat tccttgggac cagtacttta tggctcaggc agtactgtta tctttacgta
    29821 gtacgtgtac acgtttagaa gtaggagcga cactcgttcg agaaaaaagg atcattgctg
    29881 gcggctataa tggagcagtc agtggagatg tccactgcat agatgaaggg tgttacgtcg
    29941 tggatggaca ctgcttgcga acgatccatg cagagatgaa tgcactccta cagtgtgcaa
    30001 aacttggtat cccgacggaa ggttcagaaa tctatgtcac acattttcca tgtttggcct
    30061 gtacaaaggc tttgttgcaa gctggtgtga aagggatcaa ttatctccat gactatcgaa
    30121 atgatcctta tgcccaagcg ttgatcgaac aattaggtgt atctgtacac caagttgctt
    30181 tagactcaca gcatttttca aaacttcaaa atgaattaac aggaaaacat gtctagcgtt
    30241 ttagaaaggc atgttcgaaa tcggttgatt tttccggcaa ttacgtcgat gattatctgt
    30301 ttgttgtatt acactgacaa ttggggttat cgtttgcttc ttgccggaat ttttttatgg
    30361 ttttcgctaa aagaaggacg ttttatcgcg tacttgtgta ttttgtgtag cataacggta
    30421 tccatttctt ataggctaca agagtattca tttttagagg aaaaaacaac atctgcgcaa
    30481 attactgtat ttacagatac gattcgtgca aatggtaatt ttttgacaat ggaaggaagg
    30541 ttatcagaga ataaacaaaa gctccagctt tcttatcaag taaacacaga agcagaattg
    30601 aaaacatggc ttaacttccg aggaaaggga atagaattga ctgctgaagg cgagttcatc
    30661 tctccagcaa aaaagcggaa tctttataat tttgaccaag aagcttactt caggaaccat
    30721 agaatttctg gacggttcca aattcaaaag attgaacaga tcaccatgca aaatcactgg
    30781 cgaaactggc ttcagagaaa aagaggatct tttatctcct ttgtccaatc tgaatttccg
    30841 caaaaaacaa gtgtatatat taattcttta ttatggggct ataaagatgc aggattcaga
    30901 gaaccagaag aaatttttag ggagagtggt ttgctgcatc tgttcagtct atcgggtcta
    30961 cacatccaat tttatttagg gtggctgtat tatttgtttc gacgtatcgg atggccacta
    31021 cacgtcagta ttattccgtt gagtctacta actatctgtt atgtttctat tgcaggaagt
    31081 tctatttctg ttttgcgtgc tagtttgctg tttttattgc gattgctagt caaattgctg
    31141 aataagcatt attccaccat ggacttattt gcagttgtct tttggcttct tttactattt
    31201 gatcctcgat ttctttttca tgcgggtgga cagcttagtt ttttcatgag ttttttgttt
    31261 atcctgattt cttttgatgg agattggttg aaaaaaacgg cctctcacgt cctgtttact
    31321 atgatcacga tgccgttgat tgtatggcat ttttatgagt ggcctatttt aggtagtctt
    31381 tttacattac tgattgcacc ggtattcaag tttctttttc ttcctggtgt gttatttctc
    31441 acactcttca atcgctggct gccggaaact ttacttttgc tgatagaaga gggattaatc
    31501 ttatttgagc gaataatcga tttttcttca gggtcgtctc tcattatagg acgtttgccc
    31561 cttgtgctat ctgggatgta tattttcgga ttactgattt gtatggatag gttgaaagaa
    31621 caacctgtga aacacttgtt ttatgttggt cttttttctt ttattttttt gactttgcca
    31681 tttttacgct tttcttcaac gatcgctttt atcgatgtcg gtcaaggaga cagtatcttt
    31741 ttgcaaagtc cttttcgaaa agaaacgatt ttgatcgata ctggaggtca gttacacttt
    31801 gaaagagaaa agtgggcgca aggtatgata cgtcctcctg ctgaaagaaa tatcgttcca
    31861 tatctaaaag gtcaggggat aagtgtaatc gataaactca tcttgacaca cgacgatacg
    31921 gatcatgtgg gagaattacc cacgttatct cagcatttta ctatcaaaaa aatttatatc
    31981 ggttggggcg ctggacgaaa tgagccatta caaaggcatt tatctgaagc tcaaaaaaat
    32041 ggtactgaga tcattgaaat caaacatggg gacaggatca gcggttatta tgatctttat
    32101 gtattgactc cttttgaaaa gggagaggga agaaatgaag attcgattgg tctatggatg
    32161 gaatataaca ataggcgctt tttattttta ggagatttga atcaagcaat ggaaaaacaa
    32221 ttgcttttga tttatcctaa tctgaaagcc gatgtagtca aattaggtca tcacggtagc
    32281 cgtacgtcaa gtaacacaga ttttctttcc catattgaag caaaacatgg gattatatcc
    32341 tgcggcgtaa ataatcgtta tggtcacccg cacagcgaag tattggagac actagaagaa
    32401 aatcagataa aaacatggcg aacggatcaa caaggaatga tcacctatcg ctggcatcca
    32461 gtttttcatc ctcatgggct agtggaaaca atgatagact agtaattctc atctgctcat
    32521 ggtaggataa tttttgaaag tggtgagtaa tttgagtata caagcagagt tacaaaaaat
    32581 acgtaaagaa aaacttgcat cttgttactt gattttagga acagaaaagt ttctccaaga
    32641 tcaagttcgg acagaaattg tcaaaaaaat ccaattagag ggcgaagatg acttaaattt
    32701 tttaagtttt gacatggaaa atgcaagttt agatgaggta gtagctgaag cagagacgtt
    32761 gccgtttttc ggagatcaac ggttagtatt cgtagagaat ccttattttt taacaggtga
    32821 aaaagtaaac aatggaatcg aacaaaatac tgatttattg acggcttatt tgaaagaacc
    32881 attagaatcg actgtattag tattctttgc gccatatgaa aagttggatg aacggaaaaa
    32941 agtaacgaaa caattgaaaa aaacagcaat tatcatcaat gtccaacagt tgaatgaaaa
    33001 agaagtacgt caatatctga tgaatacctt agagaattcc tcaatcaaaa tggaacgaag
    33061 tgcaattgat ttattcttga ggttgaccga cttagattta tccaaaatga tgagagagtt
    33121 acagaaattg gtcctttatg gacaaaatca acagcaaatc acggtaagag aagtagaaga
    33181 attagttcct aaaacgttag aacataatat ttttgacatg acccaatata ttttaaaagg
    33241 gaagacggaa caagctttgc gtttatacga agatttagtt atgcaagggg aagaaacaat
    33301 caaaattaat gcgattttgc tttctcaatt acgtttattt ttacagacaa aatttcttgt
    33361 aaagattggt tatcagcagg caaatattgc tgaaacattg aaaatccatc catatcgtgt
    33421 aaaacttgcc atgcaggaag taaaaaaatt cgatgaacat ttgctggtac agttgtttga
    33481 tcaattagtt gagatggatt atcaaatcaa aacgggacaa atcgaaaaag aattaagttt
    33541 tcaactattc gttttgcgca cagggcagtt gttaagaaat cgataagaag aaagacgagg
    33601 gcaaagcatg cgggaaggtc gtttgttttt gtcctttttt tgacaaataa aaaaatcggc
    33661 cgaagccgat tcttttattt agcgattttt ttgcttaaac gagatttgtc gcggttagct
    33721 ttgtttttgt ggattagacc ttttgattca gccatatcaa tagctttaac agcttcttta
    33781 tataacgcat ctacgttgtc agcacctgaa gctacagcat cttcaaattt tttgattgca
    33841 gtacgcatag cgcttgtttg agatgaattt ttcacattag cgttttcgct tgtacgaaca
    33901 cgtttgattg cagattcaat gtttggcatt agtttcacct ccgataactt aagttcttac
    33961 gcgtgttacc gcataattca acattcatta ttatacctaa aggcagcgtg ctttgcaata
    34021 tgaacatgac aagttttttt ccttaacagt cttttattag gatttttttt cagtaaaaac
    34081 ggatagtaca agctgaaaaa aatcaaaaag acgctattgt gggaggcaat agcgtcaaac
    34141 caaactagat ttttatttat ctatttaaac aaagtttgaa tgggaatgtt tggtactaag
    34201 ttattatata acaaagagaa ccgtttgcaa agtttttttt gaaaagggat acgattccct
    34261 gttgttcttt gtttttctat taagaaactg cagttatttt ataaccagta tcctttgatc
    34321 agtaaaggct attctggtgt accgattatt ttaatgtatt catcgatatt ttttatcaac
    34381 atttttttta tgaccaactg gttccgacgg ttccatttgc cacttacttc cagcacatat
    34441 ttatttaggg gcaaacgtaa gatatatttt gccaaatagc gatctttaag caggccattg
    34501 atcacacggt cctcaagctg caagttgatc ccagtcagca tcattggctg ttcttgtaaa
    34561 acgtacgtat caattaaaag accttcaaaa gtttccacaa gcgtcttcct ttcttttcaa
    34621 agtaacctga aaacaggcac taattcttcc attttgatat tcgttgaagt ggatgcgatc
    34681 attctttctt ttgtccactg taatctagta taacaaaaaa gagcagaaga gccaaaaaga
    34741 aaaaagagca tcgaattcta taggaagtct cattggcacg gcctaaatta ttacaaaaca
    34801 ttgattaaaa aaagcccctg catcgcagga gcatccccta tatcgtttta ataggtcaat
    34861 caaaaagcag tatcaaacac attttgtatc aaatcaggaa ataatagata agctaagccc
    34921 agaatcaaaa gtaccgctag aagaaaccag ccgattgttc ctatgaaata ctttaatttt
    34981 tcgaacattg tatcacctcg ttgtcagtat atcacggatc agtaaattgg gggtgttaag
    35041 aatctcctaa aatagacaaa atttgttcta ttttcctaaa gcaaatactt gtaagataca
    35101 gtaagtgata ccagaagcaa tgaccggtcc ggcggcgatt cctcttaaga agacaacacc
    35161 taaaatcgtg ccgaaaacaa gtgcaacagt gatttgtgga tcagaagcaa tcaatcctac
    35221 acccttggaa gataagacag ccactagccc accgcaaaaa acagctaccc agccaatagg
    35281 ggatttaaag gcttctaata gatcacgtag accgatttct cctgttgcga tagggattag
    35341 aatagccgct gtgatgatcg tcaccccaat attgattcct ttgccttgaa gccaagggaa
    35401 aaaggaacgg gaggctggta tgagcttcat aatcaaaaca aaagctgccg ctatcatcag
    35461 actttgattt ttagctaaaa ttgctacaag taaaataatt cctaaaaaaa gccaactttc
    35521 catgaaatat attcctcact ttcttcgaaa caatcataac atgtttttag aaagttacaa
    35581 aagtaggaat tcatccttca agacctattc ataagctgtt tttttcataa aataaacaaa
    35641 atcataaaaa aacactttgc ttatttgaca gaagacagcc gcgctttgta tagtggacaa
    35701 gacttacatt tttttgaaga atcagaaaga tcaggtaaag gtcttagtaa acgcaagcca
    35761 ttcagaataa ccaatattgt gcttccttcg tgtcctacaa ctccaagagg tagatttaaa
    35821 aattgcagga agttagaagc aatcaacaag agaataacaa gaatcgaaaa aataatattt
    35881 tgtttgatga tccgccgcat ttttaaagat agccgatgac tgtagacaaa tttatttaag
    35941 tcgcttttca ttaagacgat gtctgcgata tccatagcgg catctgtccc ttgccccata
    36001 gcgacaccta aagaagcggc tgctaaagca ggcgcatcat tgactccatc accaaccatc
    36061 gcatttactt ggtaaagttc attctctttt ttgactagtt gtgttttttc ttccggagta
    36121 cagcttgcat agtaatgttc aatccctact tgttcagcaa tggcctttgc tgtgccagaa
    36181 tggtcgccgg ttatcatact tgtatggatg ttttgggatt taaagtagga aattacttgt
    36241 tgggtatttg cttttggaat atctagcaga cctaaaacag caattaactg ctgatcttta
    36301 gaaagataaa tcacggtttt gccttgattt tccaaacgtt cgatcatttc cgcaatctct
    36361 ttgctgatta tcatcgtctc aggatcatat gcgtgcttgc caactttcca ttgcgcacct
    36421 aaataggtag tttgtaatcc aaaaccagta atttcttcta ttggtaagta ttggatttct
    36481 tcaggtattg tgcaatcgaa atattgcaaa atcgcatgag caagtggatg cgtcgttttt
    36541 tgttccatag caacaagaag ttgttgtgcc aaatcctttt catcaaagaa aaagctgtca
    36601 gttacaactg gttttccttt agtgagcgtt cctgttttat caaaggcaat cgcttttaat
    36661 tcagaaagct tttctaaatg gataccgcct ttgattaaaa tcccattttt tgcagaattt
    36721 gaaagagcag ctagagaagc tggtgtagca gatgcgacaa gcgcacaagg agaagccaca
    36781 actaataata ccattcctcg ataaaagctt tcatcccaac tccaacctag gaaaaagtgg
    36841 gggagaagga tcattagagg aacagcaagc aaaatcaatt ttacataatt attttcgatt
    36901 cgttcgataa atgaagctgt ttgagttggc gtattctgcg cttcttctac taattggata
    36961 atcttagcaa agacagtttc ggaactcgtt tttgttactc gaatcgtgat gggatttcct
    37021 tgattcagcg ttcctgcata catctcatcg cctaaatatt tttctacagg cacagattca
    37081 ccgcttaatg cagactcatc gatcgttgcc tgtgtgcttt ctaaaaagcc atctatcgga
    37141 acagctgctc ctttagcgac aaataccaag ttatccacct ttagttgaga aacatctact
    37201 tcttgcattt gtccattttc ttttaataat tgggcttttt gtggttgaag attcatcaaa
    37261 gcagtaattt ctttttgact tttattagtt gtgtattcct ctaatgcacc gcttaaacaa
    37321 aagatgaatg tcaacattgc tccttcaaag taattaccga tcaaacaggc accaatagca
    37381 gctaaagcca tcaataaatc cacattcagt gtccgatcat tccacaattc tttcaagcct
    37441 tccgatgttt gtttatatcc accgatgata atggcactga tataaaaaag tggggtgatg
    37501 gtaagagaga atcggtcgaa gataaaaccg aagatcataa ataaaaggca gccgattgtt
    37561 gctgcaaaag cttttttctg attagtcatc gttattagac ctccttgtca aaacaaggat
    37621 agcataataa aaatgtaatt gataataata acagctaaat gagaacgata atggttatca
    37681 tttggtgatt aagcttgatg ttgtttttat tatttataat gattattatt aaagaaaata
    37741 aaaaaccaga acatttctca aataagagaa gtgttctggt tttttctaca ttgcaataaa
    37801 ccaataacca atcaaaatga taccaaggat aatacgatac cagccaaaag cggtaaagtc
    37861 gtttttcttc aaatagctta gtaagaattt gatagctaag acggaaacca caaaagatac
    37921 gaatgttcct acaagaagaa caacagtttc ttccattttg aatgaattcc cttcagctaa
    37981 atatttgacg atcttcaagc cgcttgcgcc aaacatgaca ggaatcccta agaaaaatga
    38041 aaactctgta gcgacaaaac gcgaagcacc aatcaagata gatcctagga tcgttgcgcc
    38101 agaacgggat gttccgggaa caagtgccaa gacttggaat aacccaacaa gaagagctgc
    38161 ttggtacgta aagtcattca gagaagtcca acgaggtgtt ttgtttttgt tgcgtttttc
    38221 gacaataata aaggcaatac cgtaaacgat caaaacaatg gcaactggta agaacttgtg
    38281 gaaatttgct tctaaccaat catccaaagg caaaccaatg atgatggctg gtatgcaagc
    38341 aacgatcact ttaaaccaaa gtgtccacgt atcttttttt tctaccgttg atttacttgg
    38401 agaaaatggg tttaacttgt gaaaataaag aacgacgaca gccaagatcg cccctaattg
    38461 gataacgaca ttgaacatcg acataaattg tgaactggca tccagtttga tgaattcatc
    38521 tgctaggatc aaatgaccag tactactgat gggcagccat tccgttattc cttcaataat
    38581 accgagaatc actgctttga taatatttgc aaaaaacata aattccttcc ttcccaatta
    38641 agatacatct tatagtatac cgttattttt tgagaaaccc aatctgtttt ttaatattaa
    38701 gaaaaacttg agaaataaag cagataagta cacgaagaga aaaaagaaga caaagccctc
    38761 gttcagactt tgccttttat gattctctgg taaaaattaa aatatcccca ttatagaaac
    38821 gagtcgcgac tatgtgtttt cccgttatcg atataagacg tatcattgat gccgtggata
    38881 gaaaatcgac cgtttgcata agtgaccttt gttacgctgc catttaaaag cccagcacga
    38941 acaggctgtt taggatcgat cagattaagc aaaaaggcga tcgtcaaacc atgagatacg
    39001 actaaaacat ttccaccacc atttttttcg cagtgatggg cgatatcttc aaatcctgtc
    39061 catacacggt ctcgcaatgt ttcataagtt tccgcccacc cagcggtatc taagttgtat
    39121 acagcattag caatttgttc aaaagaaact tctgtggtaa acatttcatc atatgtttta
    39181 aaattcaata cacgagggat cacaccccac atttccatgt catatccacc ttcaagagac
    39241 ccgaaacacc attctctaat ccttttttca ataaaataag gaatttcttt tcccttagaa
    39301 tgttctccta gaataatacg ggcagtttcg attgcacgac cactgtcact agaataagca
    39361 aatgaaaagt cgacattttt taacccgcga cctaaataat gaatgccttc ttgtccttgc
    39421 tttgtgaggg gtgtatcaca ccagccttgt actcgttcaa ttgtattgaa catcgttttt
    39481 ccgtgtcgta cgatataaag tgtagtttct gtcattttat tctcctccta agtcgtttta
    39541 acgattgaaa aaaggatttg tccttttttc ctgttcaatc gtcgttgcat cgccatgtcc
    39601 tggatagact gcaaattcat caggtaatac aaacagctgg gtccgaatac tatgaagtaa
    39661 ttgttctaaa ttccctgtat acagatcggt tctaccgata cttcctctaa acagggcgtc
    39721 gccagaaacg acaaaatcat caaaaataaa gctgacgctt cctatagaat gccctggggt
    39781 agggacgacg cgaaaagaca tgtttcctaa agtataagtt ttcatttcaa attcatattc
    39841 tgcaggcgag acaataacat cagcaatatc atcatgtcta ccgagtccag aaagattcaa
    39901 aatcggatca cctagccatt tttgctctag tgggctgaca taaactggga tttgataata
    39961 cgcgcgtaaa ggttccactg caccgatatg atcgtagtga gtatgggtaa gcaaaatcgc
    40021 taccggctgt tggtttgttt ttttgatttc ttgtttgatt cgttccgcat cttctccagg
    40081 gtcaatgatc aaaagtgctt cttcgttgta gaccaaataa cagttttctt caatcattcc
    40141 tgtttttaat ctttcgatat gcatgtgcat tcctccttga tagccaaaac ttctttcatc
    40201 cattagtata acgcattcat tgctaggaaa caaaaacagg tacttatttc tctttactta
    40261 tctgaattat cagactatat gaaaagtatt tggatattct aaagagaagg aaagtaagtt
    40321 ttcatagcat tttaatatgt ccttgttaca ttagattcat ccaaaaatcc aaattctata
    40381 tcctatatcc ctgcctaatc agcagggtgt ttttttatgt ttggaaagag agctcttttt
    40441 gctaaactag gttttttcag gtaatcttag agagaaagag gtggtggaaa tgatcaaaca
    40501 gtcaaaaata tctgtgcggc acttagtaga atttattttg cgacgtggaa gtatcgacaa
    40561 tcgtaaaaaa agtaaccata ctgcattaga aggagcgaga atccaccgca agctccaaaa
    40621 agatgcggga gaaaactatg aaaaagaagt ctttttaaaa acaacagttc aattagatga
    40681 atattacctg atcgttgaag gccgtgcaga cggtattttc aaaagagacg gtatttatta
    40741 tatagatgaa atcaaaacct ccgaaccacg atttgaagat ttagaaccag aacaagtgga
    40801 acttttcttt catcaagcaa gggtgtatgc ctatatctat agtcaagaac atgatttaca
    40861 agaaataaat ctgcaattga cttacttcca gacggcagaa gaagtgatca cgagaaaggt
    40921 tgaacatcag acaagagagc agttagaaac tttttttaaa aagctgactg aagattataa
    40981 agaatggctt gtttttcaag aaaactggcg cactgtacgc aatgcctctt tgatggcttt
    41041 gaaattcccc catgatgagt ataggaaagg tcagcgtgaa ctagcggtag ctgcttataa
    41101 aacgatccgt acgaaacaga aattgtttgt agaagcacct accggaacag gaaaaacaat
    41161 ctctacgtta ttccccgctt tgaaagctgt aggggaagaa gagggagaaa agatctttta
    41221 tctaacggct aaaacaataa ctagacaagt agctgaagat gctatgacag cattaaaaga
    41281 tacaggagct gaagtaaaaa gtgtcacttt gacggcgaaa gacaagatct gctttttaga
    41341 tgagacgacc tgtaatccag atcagtgccc atatgccaat ggttattaca atcggattaa
    41401 tgaaggttta tgggatcttt tgaatcatga gaatcagatc actcgtgaag tcatcgaaac
    41461 ttatgcaaga aagcatacac tttgtccttt cgaactatca ttagatgtca gtatctggtg
    41521 tgacgtaatc attggggatt acaattattt atttgatcca actgtttatt taagacgttt
    41581 ttttgaagat gaaaaaaacg aggactatct gtttttgatt gacgaagcac ataatttggt
    41641 gaatcgttcc cgagagatgt attcggcaga actatcttat gaaaaaacga aacgaagtaa
    41701 ggaagctata ccaaaggaat tcaaaaaact tcatcgccgt ttcaacaaac tattaaaaga
    41761 atttgattcg atccgagaaa tagcaaaaga agatcattgg gattatcatc atcaaaaagc
    41821 accggctgaa tctttagtaa aggcaggtta tcaattaagc gaaaaaatta aagaatggct
    41881 ggcagaattt cctgagcacc catcacaaga acaattgctt ccttattatt ttgatttgct
    41941 tcattttttg aaggtcagtg aattttatga tgatcactac gaaacaacag tcgaaaaaac
    42001 gtatcgcgat ctgatagtaa aagagttttg tattgatcca agcttgtttc ttgagcagtc
    42061 gttggataaa ggaaaaagca gtattctttt ttctgctagt ttttcaccac tctcttatta
    42121 tcaggaaaca ctggggggac aaaaaagtct ggcttacaaa ctgccgagtc ctttcccaga
    42181 aggaaaccag caagttttca tcgcaaatta cctagagacg acttatcgaa aaagggaaca
    42241 cagtctggca aaattagtag agacgattca tgcttttgct gatggaaagg tcgggaatta
    42301 tttcgttttc tttccttctt atcaatattt agatcttgcg gtggaagcat tcagacagca
    42361 atatcccgac agcaatgtgc taatccaaga aacggacatg aatgaggaag aacgcgaacg
    42421 atttttaaca aaatttcaaa caaatcccgc tgaaacattg attggatttt gcgtacttgg
    42481 cgggattttt tcagaaggaa tcgatttgag aggggaccga ttgatcggca gtatgatcgt
    42541 aggtgtaggt ttgccgcaga tgaatcatga gcaggaactg atcaaatcat attatgatga
    42601 gaaggaacac cttggatttg cttacgctta tcagcttcct ggaatgaaca aagtcttgca
    42661 agctggtggt cgagtgattc gtggcatggc ggatcaagga atcgttgtat tggcagatca
    42721 acgattttct agtttttctt atcgacgttt attcccacaa cattggcaaa cagcaagaga
    42781 agtacgaagt gttggggaat tggaagaagg aatacagcgt ttttggttat caaaagaagc
    42841 agtaaatcaa aaaaacgagg aatgacagac gtcagcctcg ttttttgatt aacgatagtt
    42901 agtgaattgc aactcgattg gtaagtcaag tttacgtaat aaagcaatca cagcttgtag
    42961 atcatcacgg ttcttacctg tcactctaat ctgatcttct tggatttgtg cttttacttt
    43021 cagtttttca tttttgatag ctgtagtgat tttcttcgcg ttttcacgat cgattccgtt
    43081 gaccaaatcg gcagtttgtc gagctttgcc acctagagcg tgttctgatt cggaaaaatg
    43141 gatatttttg attggcacat ttcttttgat cagcttacca aataatacat ctttgatctg
    43201 ttcgattttg aaatcatcat cgctcaatac aactagattg ctgttctcta atttgatttc
    43261 tactgtagaa tccttgaagt caaagcgatt ggacaattct tttgttgttt gctgaatcgc
    43321 attttttact tcttctgtat tgacttctga tacaatgtcg aagctagcat cttttgccat
    43381 aggaaccctc ctgatataca tataaatatt taccatacat ctatcttatc ataggattag
    43441 ggcagattct tacctgtttg ctaaaattct tctcatttga gttcgaagta ttttatttta
    43501 atagataaac tgttattctt acatttggaa taccaaagta ggggagaggt gcttgtgagt
    43561 aaaattatga ctgcgtatca taaaaatgaa agattaaaga aaatagtggt tattttgata
    43621 accggattga ccgctgcggt cgctttaaat tattttttga ttccagccaa tgtcttttct
    43681 gcagggatga atgggatcgc tcaaatcatt tcctcgcttt tatatgaatg gtttcatatc
    43741 aagatagaca caggaacatt tatttttctt ttgaatatcc ctgtgtttgt attagggttt
    43801 ctcaaagtag gaaaacgagc aacgatcctt agttttgtga atgttgtatg tgtatcggtg
    43861 atgacgatgc tgcttccagt cggacagatc actgataata ttttgatgaa tgccctagta
    43921 ggcggtgttt tattaggtct tggcgtagga ttatctttaa aaatgggctt tacaactggt
    43981 gggatggata tcatctctct tgtactttca caaacaactg gaaaaacagt cggaaactac
    44041 atgctgattc taaatggtat cattgtagtc atcgcaggct tcttctttaa ctgggaaagt
    44101 gccttgtata cgattatttc catttatgca atgactcaag taattgatgc catccacacg
    44161 agtcatcaaa aagtaacagc aatgatcgtt accgtcaagc cagaagaggt aacagaagaa
    44221 ctagcaaaac ggatggtacg tggaatgaca ttactgccgt ctgtcggtgg ctattcaaaa
    44281 aaacaaggga acatgatcat gatggtcatc acccgctacg aaatgtatga tcttgatcag
    44341 atcgttcatg aaatcgacga cgatgcattt atcaatattt taccaactca ttcagtattt
    44401 ggccggtttg caaatgaaaa cgaacaaaaa atgtatcgaa caactggcgt attgccagaa
    44461 ataaaaaatc gttcacaaaa aaaatgagga atcaggttta ttttagaata aatttaatct
    44521 attacatcta ataagaacat ttttataaat ttaaacgaga atcttacttc taaattctca
    44581 tttacaagca gctaaaaaaa gagtatgata aataaaaatc gtgttaagga gtgtcgtacg
    44641 atgagcgaag caaaccaaga acggtctgga caagaaatag aactaatcaa agaacgtatt
    44701 cttgctgcct tggaaatggt gattgatcct gaattaggaa tcgacatcgt caatttaggc
    44761 ttgatttatg atatagaatt caatcctgaa aatggagaaa cagtcattaa gatgactcta
    44821 acaactatgg gctgtccatt agcagatatt ttaacagaat ctatccatga cgcattaaaa
    44881 gaagtgccag aagtaacgaa agcagaagtc aaactagtct ggtatccagc atggacgaca
    44941 gataagatga gccgttatgc acggattgcg ttggggatac gatagaaatc ttggctgact
    45001 agctatgaga agcaaatagg aaaaacaaaa aagcctttta tcctttctag aagaaaagag
    45061 ataaaaggtt tctttgttta taaaattttg tctgctatat tataagttaa ttaaataaat
    45121 gtgttctatt gataaaggac attcaaatcc ctttcctttt ggaatccttc ttttttacat
    45181 cagtttgatt ttcgtatagt attctttctc atcttcacga taaatcgcag ccccattttt
    45241 ttgttgtgct ttaagactgc caagattttt tttatttaca gacaaaagtg cctcatcaat
    45301 gccgattttt tttgcctctt gtagtactaa tgctaatcct tgtgtggcat atcctttttt
    45361 acgataatta ggggagatgc cgtaaccaat atggccagcg ccgtttctta atgtttcgtt
    45421 cagtttatgg cgcaaattaa aaattcctac atactcatgt tcatcacttt ctaaaataaa
    45481 gacggttgcg ggaacgtaac tctcaggaag atcgattcct aaagacgttt tttcgcattt
    45541 ttttacataa tctaaaaatt cagcaaatgt atagccataa gcattgttga taaatccact
    45601 ctcatttggt ccaaagctag tttgtagctg ataagctttc tttaaatctg cttcccatag
    45661 tttgatcagt ttcatcgtat tcacctcttt aagtaacctt attcatttgt tttctactat
    45721 agcactattg ttgtaaatag ccatgcctat ttttatttat tgtgtcatat cgtattaaga
    45781 atcatcgaac aatacaaata tgaattacaa taaaaaaatt atttgaagtt cgttcattcg
    45841 ttacttatga tctctagaag aaagaaaacg tgaattactt cagcgtctag aattccagaa
    45901 ggatagtaat caacagaaga aataatcaat atccaaatga aagaagatag ctgaatcgag
    45961 aaaaagagga aaaaagaaat gaagaaaaaa ataaaatagt tactaatccc agagtcgttc
    46021 gtttaacatt gaaatcgatc atatggataa aacagatttt ttcaccaacc ctcaatagat
    46081 gatggagttg ctcggatgaa ttatttgtgt agatggcata agggagggcg tacagaaatg
    46141 aaaaagaaca aaaatatgtt cttgacaaaa aacacatgta atcctaaact aaaagatgtg
    46201 atgcggctat ggcggaattg gcagacgcgc tagcttcagg cgctagtgag ggtgacctcg
    46261 tggaagttcg agtcttctta gccgcataat ttgttttttt ataaagagaa ggtacacctg
    46321 gagcgatgag aaagaaaata acatattatc taaaagaata taggaaaaat tttaaacaac
    46381 ggaaccagat tcaaataaat caaaaaaaat ttgtgtgaag gtgggacagt ttaaaaccta
    46441 atagattgat tcaaatagta gatttataga ttctgataca agaaatactg gcaaacgata
    46501 gttaaggaat aaagactaaa cttaaactca aaaagaggtc atgcgaaaga ataaatcgca
    46561 tgacctctta ctatattttg aatgtgttta aacgttcaaa taatgaatct tagtaccaac
    46621 cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa gtaacatatt
    46681 gttctgctac tctttcttgg tttgcagcag agtagtcacc atttaagtaa gaagaatcta
    46741 attggtaacg gccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt gattcttttt
    46801 gtgcgatcca ttcttttgca gagcttgtac ttgctggagt agcagcagtt tctgttacag
    46861 caggagcagg ttgtgtttca gcaacaggcg ctgcttgtgt ttccgtttga accgtttctt
    46921 gtacaacagg ttgttcagct tgaacaggtt cagcttggac tggtgcttct tgaacggctg
    46981 cttgaggttc agcagctgct tgtgcagagc cttctgttgg gattgttaat tgttgtcctg
    47041 agta
//
LOCUS       DANKMN010000012        44824 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000012 DANKMN010000000
VERSION     DANKMN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            42..1739
                     /locus_tag="PW224_000677"
     CDS             42..1739
                     /locus_tag="PW224_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907388.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            complement(1784..2788)
                     /locus_tag="PW224_000678"
     CDS             complement(1784..2788)
                     /locus_tag="PW224_000678"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL2907389.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(2799..3539)
                     /locus_tag="PW224_000679"
     CDS             complement(2799..3539)
                     /locus_tag="PW224_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2907390.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            complement(3609..4412)
                     /locus_tag="PW224_000680"
     CDS             complement(3609..4412)
                     /locus_tag="PW224_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2907391.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            complement(4412..5494)
                     /locus_tag="PW224_000681"
     CDS             complement(4412..5494)
                     /locus_tag="PW224_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL2907392.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            complement(5635..6903)
                     /gene="dltD"
                     /locus_tag="PW224_000682"
     CDS             complement(5635..6903)
                     /gene="dltD"
                     /locus_tag="PW224_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL2907393.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            complement(6906..7139)
                     /gene="dltC"
                     /locus_tag="PW224_000683"
     CDS             complement(6906..7139)
                     /gene="dltC"
                     /locus_tag="PW224_000683"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL2907394.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            complement(7184..8389)
                     /gene="dltB"
                     /locus_tag="PW224_000684"
     CDS             complement(7184..8389)
                     /gene="dltB"
                     /locus_tag="PW224_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL2907395.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            complement(8386..9900)
                     /gene="dltA"
                     /locus_tag="PW224_000685"
     CDS             complement(8386..9900)
                     /gene="dltA"
                     /locus_tag="PW224_000685"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL2907396.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            complement(9911..10060)
                     /locus_tag="PW224_000686"
     CDS             complement(9911..10060)
                     /locus_tag="PW224_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL2907397.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            complement(10341..12383)
                     /locus_tag="PW224_000687"
     CDS             complement(10341..12383)
                     /locus_tag="PW224_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907398.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            complement(12373..13167)
                     /locus_tag="PW224_000688"
     CDS             complement(12373..13167)
                     /locus_tag="PW224_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907399.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            13466..15655
                     /gene="nrdD"
                     /locus_tag="PW224_000689"
     CDS             13466..15655
                     /gene="nrdD"
                     /locus_tag="PW224_000689"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL2907400.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15970..16572
                     /gene="nrdG"
                     /locus_tag="PW224_000690"
     CDS             15970..16572
                     /gene="nrdG"
                     /locus_tag="PW224_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL2907401.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(16626..17747)
                     /gene="dinB"
                     /locus_tag="PW224_000691"
     CDS             complement(16626..17747)
                     /gene="dinB"
                     /locus_tag="PW224_000691"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL2907402.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(17856..18728)
                     /gene="rsmI"
                     /locus_tag="PW224_000692"
     CDS             complement(17856..18728)
                     /gene="rsmI"
                     /locus_tag="PW224_000692"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL2907403.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            complement(18797..19144)
                     /locus_tag="PW224_000693"
     CDS             complement(18797..19144)
                     /locus_tag="PW224_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL2907404.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            complement(19137..19961)
                     /locus_tag="PW224_000694"
     CDS             complement(19137..19961)
                     /locus_tag="PW224_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL2907405.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            complement(19997..20935)
                     /gene="holB"
                     /locus_tag="PW224_000695"
     CDS             complement(19997..20935)
                     /gene="holB"
                     /locus_tag="PW224_000695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL2907406.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            complement(20949..21278)
                     /locus_tag="PW224_000696"
     CDS             complement(20949..21278)
                     /locus_tag="PW224_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL2907407.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            complement(21293..21937)
                     /locus_tag="PW224_000697"
     CDS             complement(21293..21937)
                     /locus_tag="PW224_000697"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL2907408.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            complement(22001..22597)
                     /gene="recR"
                     /locus_tag="PW224_000698"
     CDS             complement(22001..22597)
                     /gene="recR"
                     /locus_tag="PW224_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL2907409.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            complement(22622..22936)
                     /locus_tag="PW224_000699"
     CDS             complement(22622..22936)
                     /locus_tag="PW224_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL2907410.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            complement(22956..24701)
                     /gene="dnaX"
                     /locus_tag="PW224_000700"
     CDS             complement(22956..24701)
                     /gene="dnaX"
                     /locus_tag="PW224_000700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL2907411.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            24916..26163
                     /locus_tag="PW224_000701"
     CDS             24916..26163
                     /locus_tag="PW224_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2907412.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            26188..27372
                     /locus_tag="PW224_000702"
     CDS             26188..27372
                     /locus_tag="PW224_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2907413.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            27369..28166
                     /locus_tag="PW224_000703"
     CDS             27369..28166
                     /locus_tag="PW224_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL2907414.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(28608..29303)
                     /locus_tag="PW224_000704"
     CDS             complement(28608..29303)
                     /locus_tag="PW224_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2907415.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            29457..29735
                     /locus_tag="PW224_000705"
     CDS             29457..29735
                     /locus_tag="PW224_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907416.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            29754..30962
                     /locus_tag="PW224_000706"
     CDS             29754..30962
                     /locus_tag="PW224_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2907417.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(31110..31475)
                     /locus_tag="PW224_000707"
     CDS             complement(31110..31475)
                     /locus_tag="PW224_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL2907418.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(31622..32395)
                     /locus_tag="PW224_000708"
     CDS             complement(31622..32395)
                     /locus_tag="PW224_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2907419.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(32569..33429)
                     /locus_tag="PW224_000709"
     CDS             complement(32569..33429)
                     /locus_tag="PW224_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL2907420.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(33429..34847)
                     /locus_tag="PW224_000710"
     CDS             complement(33429..34847)
                     /locus_tag="PW224_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2907421.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(34904..35353)
                     /locus_tag="PW224_000711"
     CDS             complement(34904..35353)
                     /locus_tag="PW224_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2907422.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(35798..36061)
                     /locus_tag="PW224_000712"
     CDS             complement(35798..36061)
                     /locus_tag="PW224_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL2907423.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(36160..37455)
                     /gene="rho"
                     /locus_tag="PW224_000713"
     CDS             complement(36160..37455)
                     /gene="rho"
                     /locus_tag="PW224_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL2907424.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(37452..38723)
                     /locus_tag="PW224_000714"
     CDS             complement(37452..38723)
                     /locus_tag="PW224_000714"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2907425.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(38889..39758)
                     /locus_tag="PW224_000715"
     CDS             complement(38889..39758)
                     /locus_tag="PW224_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL2907426.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(40050..41660)
                     /locus_tag="PW224_000716"
     CDS             complement(40050..41660)
                     /locus_tag="PW224_000716"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL2907427.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(42853..44193)
                     /gene="glnA"
                     /locus_tag="PW224_000717"
     CDS             complement(42853..44193)
                     /gene="glnA"
                     /locus_tag="PW224_000717"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL2907428.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(44221..44613)
                     /locus_tag="PW224_000718"
     CDS             complement(44221..44613)
                     /locus_tag="PW224_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2907429.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT        13262 a         9338 c         7822 g        14402 t
ORIGIN      
        1 atttctttta ttttattttt tctcctgcgg acagcaatca aatgaaagaa ataaaagatt
       61 cgtgggtttt ctatacaaaa gatgatcctg attttaagtt tgacagcaag tacactcgta
      121 cgattcctac cgtggataaa gacgaaacat tcattatgga aacgacactt gaaaaaccgt
      181 tggacgaagc aaatttacta atcaaaggaa accatcaatg gattactgta tatctaggcg
      241 aaaagatttt ataccaacgg aaagattatc aggaagaaag caatcccgga ctctcattgg
      301 ctgtgataga tttaccagca aattatatcg gaaaaaaatt gacccttaca gtctcatctc
      361 cttatcagaa ctatgctgga ctgccaccga aagtttatct aggaacaact ggaccattga
      421 ttagttttat ttattcacaa tcagtgccgc aagtattgac gatgataatc gctgttttta
      481 tcgctatagg gacctttttc tatacgatct acttgatgta taaacaaaaa cgtcttgaat
      541 actcacttat tctcttaagt tgctttgctt tggcattagg atttgaagct gtatcagaag
      601 atattttaag tggtattctt tttgaaccat tcgttcattc ttttttatct catttattta
      661 ttatcttaac gtcagctttc atcatctgct actattggag ccgaatgttt tactataaaa
      721 aatggtacgg aattttttgt acgttccaac ttttgatttt tatcggcgtt ctgctttatg
      781 caacttttac tcctgcagaa cttcctgaat taatgccgat tgctaatatt gctagtgtta
      841 taagcacttt agtcacttca cttgcatgca ttggcgaagc atataagaat aaccgttttt
      901 atgtagcttg tacaccttgg atcgtcttgg tagccattat ccattgcttg atttacatcc
      961 aaacggcatt aggtatctat caaacaacga tcaattggtc tacgatttta tatattatca
     1021 ttctaatcgt tattatcagc tacaatctaa tcgatcacat cttaaatacc gacaaagatc
     1081 ggagacaagt agatttctta aaaatcaaaa atgatttgtt ggagcaacat tatgagcagc
     1141 ttcgacagca tatccaagaa gtaaatacac ttcgcctgga atttgtccaa gagatggaga
     1201 atctgcaaga tttaatgcat acggaccaaa caaaagctaa aaactatgtg gaaaagatat
     1261 tagaagaagc aaaaaatttc gaaatgctgt gttctttttg caaccaccag gtgacaaatc
     1321 ttatcttagc gcgttaccag aagttagctg ccaaacggaa tattcagata accttccaag
     1381 cagatcttcc cgaagaactt cctatcaacg atgacgatct ggctcaactt ttgatccacg
     1441 ctttagagca ttcctttaga gaaactcatg ccatcgaaaa tccactgtat cgaaaaattt
     1501 atttgtcgat acacgaacaa gaggaacatt tcgtcatttg ttgcgaacat agtgcacatt
     1561 acgatacaaa cctatttagt cgaggcatca cagaagaatt agatgatcag gaacgttttg
     1621 acttaatgat gatgaaagac gtagcggatc gttacacagg aacactaacg caggaaaaag
     1681 atactttcat tgatcggatc actgttcaac tctctcgaaa ttacccgaat cctgtataaa
     1741 aaatagcgga atccatatat gtggattccg ctatttttta ttttcaataa agtaaggtaa
     1801 gcaattcttc tggttctatc gcaccaaaat atttcttcac atcaatttgg ctgactattt
     1861 ttgatagaga tgctttatca tatttctgtc caaccaattt tttttccacg tcagagatat
     1921 ctcctagtcc gaagaaatcg ccaaagattt ttgcttcttt gatcattcct tctgagacat
     1981 tcaaatggac ttcaatcgaa ccgatagaaa aacgtttttg gcgagagaaa ttgaaatctg
     2041 gagacttccc gtaattccaa tcccaatttc gataatattc ctcagagatt cgattgattt
     2101 ttttccaatc ttcatcagtc aattcgtacg tttttacttc ttctactgac tctacgccga
     2161 aaattttcaa caagatttct tgacgaaatt cttctgttgt cattttttgc ttttcttctg
     2221 gaagaaatgg tttgatattc gttacacgag aacggatcga tttgattcct tttgattcga
     2281 ttttgtcttt ttttactttt aatgcattga ctacttcatt gatatcagag tcgaacataa
     2341 ttgttccatg agcaaacatc cgaccgttgg tagcatacat cgcgttgcca gaaaatttca
     2401 tgccatcaat gactagatca tttcggcctt tcaattccgc cccagcaaca cccaattcat
     2461 gaagtgcttg gatgattggc tgtgttactt tcgcaaagtc acggaatgaa tccccatcat
     2521 caggcatgat aaaactaaag ttcaggtttc caaaatcatg gtagacagct ccgcctccac
     2581 ttaagcgacg gacaacatga atgccatttt cttccacata atctcgattg atttcttcaa
     2641 gcgtattttg gtttctgccg atgatgattg aaggttcatt gatataaaag agcaaaatag
     2701 gttcatctaa aggcatttct tgcagcaaat attgttcaat tgctaaattg attcttgggt
     2761 cgtggttttc atttggtaca aaaatcatgt tcttcctcct aaatgttaaa cactttgtca
     2821 actgcagcaa gttctatatc aatccctttg cctgctgcct tttgcgcttc atttttcaat
     2881 tgttctaaat caccaaactg aggtaggtgt gtaagtacaa gtttctttac gttagctgat
     2941 ttcgcaagtt ctccagcttc ttttgaagtg aaatgggcat gatgatgttc atttccttca
     3001 aaaagatagg tatctgctag aaacaaatca gcatctttcg caaaatcagc gaaactttcc
     3061 aaataacctg aatcacctgt aaatacgaat actttccccg ttgtttcttc tacgatacgc
     3121 atggcgtaac atactactgg atggattgtt tccataaaag taatcagaaa aggaccaatt
     3181 tttaattctt cagcttcaaa ataaggatgt ccttttgaga cattttccaa cgtcaattct
     3241 tcaaagtgaa tggaatcttt tgtgtgtcca taaattgaaa gcaatgtctc cggatttctc
     3301 ttaggatgaa gttgccagta atactgtaat acaccaaggt cagcaatatg atcatgatga
     3361 taatggctaa ggatcacagc atctagatca agtggatcaa ggtgttcttg taatttgatc
     3421 agcgtagcac tacctgcatc gatcaacaaa ttgaacccgt cagattgaag caaataacct
     3481 gttgtccctt gatttttata aggatacgct cctaagcatc ctaaaacagt taatttcatc
     3541 gtacattccc cctcaaagat ttatcctacc acaaaagtta tttttttgat gaataaactg
     3601 cttacatgct aagtaaaata ggcgtctgga cctccttttt caatccgtgc ttgttcataa
     3661 aattctgcaa ctgacacatt cacataaaca atgacccaca aaagaccgat gaacaaagcg
     3721 attgctccta gcaaatacca gccgataaaa gacagctgca acaaaatata cgtaccaatt
     3781 ctatctttca ataaaaacca gccatatttc aaacattgaa taaccgacaa atcaggatag
     3841 tcgaactttg tccatgcagc aaattggaat aaaccgccaa caatactcat gatgagtgct
     3901 gaaacaaaaa tcatccctat catcgataaa acaaagctga tatcaagaaa atcttctggc
     3961 atgacacccg taggtaccgc atttgaactg aaaagcagct gcatataagt actcactccg
     4021 ctgattaaaa acatgggcag aaagattaaa ctgttgagta gccagttgat gactgaattg
     4081 atcaaattga tgccaagtat cgaaccaaaa tactttcttt ggaaaactga aaataaaagg
     4141 ttactttttg gtctgccacc ccgtacaact actagcgcaa tgtaaaacaa tgcatagttg
     4201 aatgcgaata aaagtaaatt atcgactaga aaactcaata agtgctgctg catgctttct
     4261 tccggaaaag gactgaaacc gtcaaaaaag ttctggatga cgctcgtaaa tattatagat
     4321 aagaaaacaa gccaagtatt gattccccat tgattttcca aagaaactcg tgcttttttc
     4381 cgatattcag aaataggtat tctattattc attattcata actccgaatt ttttcaactg
     4441 tttcactatc aagccgtttc actaattctg tgactagttt cactgtattc aaataatcat
     4501 cttcatgaat cactgatgta tgggaatgca gataacgaac aggaacggta atggctacag
     4561 acggaacacc atctcttgtc aaatgcattt gtcctgcatc agttcctccg ccagaaatca
     4621 ctgtatattg gaatggaata tccatttcct ctgctacatc aataacaaaa ttcaataatt
     4681 ttttatgcgg gatcatagat gcatcaaaaa tcaaaatctg tgggcctttt cctaaaatcg
     4741 aatctgcttc ttttggtgtc attcctggag tgtctcctgc tgtaccagta tctaaagcaa
     4801 aagcaatatc gggatttaca aggtgtgtac ttgttcgagc acctcgcaag cctacttctt
     4861 cttgcacatc gcttcctgcg aataaaacat ttgggtgtgc ttctttagat agattctcta
     4921 atactcgcag agaaacagct gtcccgatac gattatccca tgcttttgcc agtaaatatt
     4981 tactgccatt cattcgtttg tattcaatat atggagtgac catatctcca ggacgtatgc
     5041 cccactcttt ggcttcttct tggcttgatg cgccaatatc aatgaacata tcctttattt
     5101 cataaggttt atttcttact tgaggtgtta aaacatgtgg tggtttggaa ccaatcacac
     5161 cgtggacgat tttcccttca cgtgttttga tttcaacttg ctgagcaagc atgacttgcc
     5221 cccaccaacc tccaagagtt tggaattcca agaatccttt ttcggttatt tttgttacca
     5281 taaaaccaac ttcgtccata tgtccagaaa taaagacctt ggggcctccg ccagcaccat
     5341 gtttggcaat cacactgcct aaaccgtcat aaaaaatatc atcagcgaat ggtttggcat
     5401 attctctgaa tacttctctt acctcttctt catttccagg tactcctttt gcagaagtta
     5461 aatcaataag tagctgttct tcttttgtat ccaaatgttt tcccccattt ctatattcat
     5521 attgtgatta taccattgct tatgattttt gttcaggcaa acttggtatt tttatctttt
     5581 attcagaata ttcaaaaata aagaaaacaa gcgagcaaca ctcgcctgtt ttctttattt
     5641 taattgtagt ttatctggta tttgatgttg ccaagatttg ctaaaaaaag catcgtcaag
     5701 atgatactta ctagcagtaa tatgattttc ttctaaaaac ggtctgatct gttgatctgc
     5761 agccaaccag cctttccagc ctagatggat cgtgtcttcc ataaaatagt tggtacccgc
     5821 ttgattgaca aaatcagcta ttcgattgaa tccttggcta tttaattgaa atttgatctt
     5881 tttggcaaat ccttgtaaca tttcttgaga tagtccggta taatcgctcc atttctcgtt
     5941 aaccggtggg atgatgaaca atacttcgtt atggtttttt gctaattgat ccaagaccag
     6001 ctgaaaatca gaaaattctg gcgaaaaacg ataatcccaa tttttttgtg actgtttgag
     6061 ctctggctcg tgggctcgga tacgatgcgt ataaaaatcg tttttcaacc cgaatggatt
     6121 attcgtcgtt tctcgttctc caatttgatt tgctaaaatg gataattctg tttcttggta
     6181 ttgttttggt aatcgttttg actcatgatc gattttttgc tgtcgatctt tcaacccaat
     6241 attgctgaat acttcatcct ctctcttcag catgttccat tggctgcgca gctgattcaa
     6301 agattcaggt aaaggtagct tccctttttt gatcgtttgc agaatcgccg tcaacgtatc
     6361 attctctttt acttttgaga aagttaataa tcttctagct aaatagcgat cagcaggcgt
     6421 tactttcttc attgaaaaca accagtcgta tgtttgaagt tcagaataat acgtattaaa
     6481 gtaatcagtt tttacaccat ttttaacgaa ccactgagga gaaataataa agacgacttt
     6541 cttgtttttc atagcatctc ctgcagaacg catcatcata tattggctta acgattgtgt
     6601 cccgggtgct cccagcaaaa atggacgata gtttcgctgg tatttttctg ccagaacaga
     6661 cggatgaaat ggactgattc tgcttaattc acttgagcca aaaaaaggaa tatattgttt
     6721 ttcagataat gcataatttt ttatcgcatc tcctttcaac acgttcactg acatggaaga
     6781 agaggcacgt ctaatcgttt ctggattaga gatatctact tgataggagg aggtaaaaaa
     6841 cacaaacagt aagccaacag ctaaaacaac cgggccgaaa atagcaaaaa gtttcttttt
     6901 catactcaac ccttcaatgc agttgcttgt tgaatgattt tattcggtgt gcaccaatcc
     6961 tctcgctcga attcagatac agggacttgg atatccagct ctccgtctag ctcgactaat
     7021 aattgaacag atgctaaaga atcaagcaac ccttcatcaa atagatcaat atcacgattt
     7081 tttcttactt catctgttcc tgttatttct tctaagattg ataaaatttg ttcttccatt
     7141 ttccttttct cctttgttat ataaattaat atgtcactat tcgttagcta aaaaaaagtt
     7201 tatctaaaaa tcctgaaaaa atcaggaagc taaaacatac tgcttgaaat gtcaaaaata
     7261 cggctaagcc atgcgtaaat ttgttagaag gcaactgctc tttatgcttc ttcttaaatc
     7321 ggatccatgc atctgtcaga cagatcagac aagcatgata cagtccatat acgatgtagt
     7381 accacgtcaa tccatgccat atacccatta gaagaaacaa agcaaaataa ccgatatttg
     7441 acattacgat cttgctttta aaaaattttt tcttcatcat gaagaacatc aatcgcatat
     7501 atacataatc acggaaccaa aaggacagcg tcatatgcca acgattccag aattctttga
     7561 tattccatga taaaaaaggt ttattgaaat tgattggtgt ctgatatccc atcatgtagc
     7621 ttgtaccgac tgcaaataag ctatagccag caaaatcaaa aaacaaatac atactgtaca
     7681 cgtacatata acctactaat gcccatgaaa taccaccatg ctgtaaggca atgcgtccca
     7741 ctacaggaag cagtacttga ccaaaataat aaccgataat gaatttatat aaaaaaccga
     7801 caaaaatatt ttggacgcct tttcccaaaa gatcaacata tttatcacga acaggcggat
     7861 ggagctgatc tttttcaaat ctcctatagc gatcgattgg tccagaagag attgttggaa
     7921 agaacagcag aaactggata taacgaaatg gcttatatgt tttgatcatg ccatctctta
     7981 tttccatgat catttgtaca gatttaaagg ttaaataaga gattcctaaa aaaccaagta
     8041 acgatgcttt tccatgttca aaaaaaggag tcactttcgt taaaaacagt ggaattatcg
     8101 ctaaaataac ggcggcaaaa aaccatcctg tctgattttt cctttgccga taaacaaaat
     8161 agccccatac aagtatcgtc tgccacaaaa catacccaat cagcgccgct ccctgttgcc
     8221 aataattacc tccaaagctg acaaataaaa agaaaagtgt cactgctgat tgataaactg
     8281 gtaatctttt ttcccatatc aataaagata gaacgatcgg tacaagcgca atggctaaaa
     8341 gaaaaaaata aaacggctct gcataaggga tcatatgagg taacatcatt caggattcac
     8401 ctcagctgtt aatttttttc gatcgatttt tccattaact gtctgaggca gcagatcgac
     8461 ataatggaac ttctgaggaa tcatataatc cattacaact tctagtaatt gttctttgat
     8521 agatttagtc aactgaaaat ccggctgttt cgttttgttt tctaatacga caaaagcaac
     8581 cagctgctgt acttttgttc cttggtattt tggtacgacg acagcttgtt tgattcggtt
     8641 gtcttggagc agataatgtt cgatatcccc gagttcaatc cggtatccat gtaatttgac
     8701 ctgctgatcc atacgtcctt cataaaataa aagcccatat tcatctagtc tacctgcatc
     8761 ccctgtatgg taacttgcta agtcattttg acaaaagaac gcagcagctg tcttttcggg
     8821 attgttcagg taaccttttg atacgcttgg tcctgcaata atgatttcac ccgtttctcc
     8881 ttttgaaaca ggtttacctt cttggacaat agaaactttc gtgtcttttt ttacgtagcc
     8941 aatcggtagt cggtcataac tatctagtac atgctggtca atctgaatac ttgaaatagc
     9001 tacagttgct tctgttggac cgtaagtatt atatatccgt gcatttggga atcgatcaag
     9061 tagactttct gcagtttttt tcgtcaattc ttctccgcaa aataaaaaca tgtccaattc
     9121 cggcacattt tctccattga atgtaggttc catcaagcaa atatccataa atgatggagt
     9181 agaaacccac acattcaact tcaaggctgg caatgtcgcg aataattgac gaaagtcttg
     9241 aacaactgct ttatttaacg gcttcagcat tccgccagaa actaatgcag gataaataga
     9301 aaataccgat aaatcaaaag aaaatggcgc ttgggctaag aaatgcatac caggcttgat
     9361 cccaaagtct tccatcgtcc attgaacaaa gctgactaga ttatcatgac tgatttgtac
     9421 gccttttggt tctccagtag taccagaagt aaaaataata taaaaatttt ctgtaccttt
     9481 tacgctattt ctgggataaa atcctactgg tatcttcttg atttcttgaa attctttttt
     9541 tgttatgaca ggaaccgaag gaatatcttc tatccagtcc ccgatactga tgaccataga
     9601 tggtttagcc acccgaaaga tcgataggat acgctccttg ggtgtatgct cttcaatcgg
     9661 caaataggca tggccagatt tgacaacccc tagaaatgag acaatcattt caaactccaa
     9721 attgccaaaa acgacaattg gtccatcgcc ttctaccttt ttttctaaat aataagcaag
     9781 tgcgtctgat gcttgtttca attcaccaaa agtatgcgtc tcatccgcct gataagcaac
     9841 tcgatcaggt tcatttattc cccaatcatc gattgcttca ataatcgttt tgatttccat
     9901 actcttcctc ctaaaattcg ttatagataa aagttcctcc ttgaatattt ttatagtgat
     9961 acagataaat caggaacagc agtactgcac tataaaaaat tgttcgaatg atgaattttc
    10021 cccaataatt caattttgga ttagaacgcc atttgttcat tggattccct cctcatataa
    10081 cctcacacaa agtgtaacta attttgacaa aaagaaacat gtgtctagct tacagaaacc
    10141 gtttttttac taacagtttt gtcacatagc ttttataaaa atagcacatc aaatcactgc
    10201 ttttttcttt ataatttctc gtacttttaa aaaatataaa aaataaagag gttgtgacaa
    10261 aaatcgtgtc acaacctctt attccaaata aatggtattc aaaatttagt tctatttatt
    10321 atcttgtctt attccgtata tcattcctcg ataatccgac aatataactg actcgtaatg
    10381 acataaagca aaagatagat aaggatcaat aagccactca caagataagc caaccagtaa
    10441 tcggcagttt ttatatactg agtgaagaca ttgattgcaa atactgcgtg agtgatccct
    10501 aatatcatgg gtggaaagaa ggtaagtgcg ttttgctcga agatcatccg tctgatcctt
    10561 ctttgtggaa tccccagctt agtcaatagg tgataatttt ctttttcttc ctctgcttct
    10621 gctaattgcc ggactgtcaa aatactacca gtagcaataa tcacgatcat cccgacaaat
    10681 agtgtgacaa aaatattgat accgattcgc cgattaactg cacgcaattt cgaatagctg
    10741 gaagagaaat tcaaacgaga gctttcttgg ctaaattgtg cttccttcat ttggtcatcc
    10801 ggaagtttct ttgtgctaat ttctccttgt agacttccat ttacgatatc ataagaataa
    10861 taaacagcat tgagccactt tatcgacagg tcattgctca gtcgttcatc tattttttca
    10921 ttatttccac ctgatacatc tacattgact acttgataat ccacaccttt tgctttttca
    10981 aaaacggtat catctacaat cagtacagga cttagatata ccattgtttc gtcacctaat
    11041 acacttggaa gcatttgttg aatcttcaat tcttgattat ctggcagtcc aatactagat
    11101 ccatataatg aaatattccg aaaaagagtt tgcatttgat caagcaaaac agtatgacca
    11161 ggtgatttca aagaaatgtt cggcaaatct ggattaactt ttctgaaggc ttgatattgt
    11221 gtttccgtga tcagattagc agtttgaaac ataggttctt cgagattttg actatagata
    11281 ctcccaatga ctttataatg caaggtgatt tctttagtta tcttctgccc ttcctctgct
    11341 agaatcggct tgattttttt ctctgtctca acatcaaact gatagtccat cggggaggaa
    11401 actttctcct ggtaggataa aatagtagag atactagccg tgccgccaat tgtcattaaa
    11461 gaaattccga gaatgactgt aatcagagaa tttgtacgcc aacttttgag caaatgaact
    11521 tgcgtgttac ccattatcaa aaaattgata ccatagtaat aaatcttcca tttactcaat
    11581 atgatcaaaa ataaccgaaa tgtataacag aaaaacaaat aagtaccgac cacacaaacg
    11641 aaaaaaatta aaaaaggaaa aaagaaaact aatgtatcaa gataatgata actgatcatc
    11701 catgaagtga tttccctaaa atgactagct ccaaaatatc cgctcgttaa aagtagtaac
    11761 ccgatgactg cgagtaatcg gtgccttgtc ctgaagcgca ttgttgaact atctgcttgt
    11821 tctttttctc caaaacttcg aatcattgga tagcgccata ttttccatat tgaatataca
    11881 gatactgcta aaaggatgat gaaaaaagca aataaagtat ctactactga tggaatggag
    11941 ataaaaaatg gactagtaag atccatatcc atcattcgaa ccaaaatcat tgaaaagagt
    12001 ttggaaaaaa taataccgag taatatccca gaaatacagg cgaacaatcc aattatcatg
    12061 atttctaaca catatatacc tgatatctgc agtttattca taccaaaaag ctgatagatg
    12121 cctatttcct tttgccttct attcaagaaa aatcggttgg cactgaatac gaaaaaaagc
    12181 aaaacaacag taatgataaa tccaccaaaa ctcaatccac catctatcga agtatcttga
    12241 cttgctcttc tgactaaaga ttgatcgtag gtcattgcgc tgaaggaata atagatcatt
    12301 acggataaag tcaaactgaa aaaataaaca agatagcctc gtgactgccg aaaaaaactt
    12361 ttaacggcta gtttatacag cattcatttt tcctcctccg ccaatcgtcg cctgcatgtc
    12421 tattactttc tcaaagaact ctcttcttgt ccctcttctg acaacttcag aaaaaatcac
    12481 accatcttta ataaataaaa ttctatcgca gtagcttgct gtatatggat catgggtgac
    12541 taacagtatc gttgcttttt ctttttgatt catggtcttc aagtagttca acaattctgt
    12601 agccgacttc gagtccaaag agccagttgg ttcatctgca aaaatcactt gtggctgaac
    12661 aacaagtgct ctagcagctg caattcgttg tctctgtccg acagaaagtt catcgggata
    12721 ccgtttgagc agttcttcta tattcaacaa ttgagctaca tgtcttactc tcttttccat
    12781 ttcctcttta gtcatccgtt caactgctag gggcaatagg atattgtctt ttgcatttag
    12841 ggtatcgatc aaattgaact cttgaaaaat gaatcctagt ttttttcgac gaaagtcact
    12901 gagctcactg tttttcattt ttgtgatttc ttttccttgg atcgagacac tgcccatcgt
    12961 aggcaaattg atcgtggaca agatattcat cagcgttgtc tttcctgcac cacttgggcc
    13021 cataatccca acaaactcgc cttcttctac tgtgaatgag atattatcta aggctttgac
    13081 gggagaaagt ttgctgccgt agcttttact tagatgtttc acttcgatca tcttttccaa
    13141 ttcttctcct cctctttcat ttttcatttc tatttatttg catagtagca tatctacgaa
    13201 taatttgctc ttatgttttt gtcactttta cttggactga ataaaaaatt ttattcaatt
    13261 attttattca gcaaaattaa atatgcaaaa aacaaatcca ccagctacaa gttttcacct
    13321 tttttcacaa taaaatatac actacatata gtatagaaat ccaattatac agcacattga
    13381 atactatata tagccgatat ttagttgctc cttccccgat tttgttttat gatagacaag
    13441 aacaatttag caggagttga agataatgaa agtaaattat gaagaaaaaa ctgtcgttac
    13501 tcagcagcca gagacatccc agatgaaagt catcaaacgc gatggccgcc atgttgattt
    13561 cgatgaacaa aagatcaatg aagcactgat caaagcagaa aagaaaattc acggctcatt
    13621 atcaccactt acttatgaaa aaattcaaat gattgcagac ttggttgtcc aagaaatcaa
    13681 aagccggttt gctgaaaatg tcaaaatcta tgagattcaa aacatcgtgg aacatatatt
    13741 actagaaaaa aacgaatatg aattagcaga agagtacatc aattatcgta ccaaacgaga
    13801 ttttgctcgc agtaaagcaa cagatatcaa cttttcgatt gaaaagctaa tcaacaagga
    13861 ccaaactgtc gtgaacgaaa acgcaaataa agacagtaat gttttcaata cacagcgaga
    13921 tctgacagcc gggatcgttg gaaaatcaat cggactgaaa atgttgccac cacatatagc
    13981 aaatgcgcat caaaaaggcg atatccatta ccatgatttg gattatcatc cttatacacc
    14041 gatgacgaat tgctgcttga ttgatttcaa aggcatgctg aacaatgggt tcaaaatcgg
    14101 caatgccgaa gtcgaatcgc caaaatctat ccagaccgcc acagcacaaa tctcacagat
    14161 tattgccaat gtagcgtcaa gtcaatatgg tggctgttct gccgaccgaa cagatgaatt
    14221 gcttgctcct tttgcagaat tgaactataa aaaacatcta aaagacgcgg aagaatggat
    14281 cgattcgcca gaacgccaaa aagaatatgc aaaagcaaaa accaaaaaag atatcttcga
    14341 tgctatgcaa agtttggaat atgaaatcaa taccttattt acttcaaatg gacaaactcc
    14401 tttcacttca ttaggatttg gactaggaga aaactggttt gaaagagaaa ttcaaaaagc
    14461 aattttacag attcgaatca atggattagg gagtgaaaag cggacagcta tcttccctaa
    14521 actgatcttc actttgaaaa aaggtgtgaa cttaaaccct gaagatccta attacgacat
    14581 caaacaacta gcattggaat gtgcaacgaa aagaatgtat cctgatattc tgaattacga
    14641 taaaatcgtt gaattaactg gaagtttcaa agtacctatg ggctgtcgtt cattcttgca
    14701 agggtggaaa gacgaaaatg gtcaagaagt aaatgtcgga cgaatgaatt taggtgttgt
    14761 tacattgaat ttgccaagaa tcgcgatcga atcaaaagga gatcagaata aattctggca
    14821 gttactgtct gaccgcttag aaatcatgaa agatgcgctt ctctatcgtg tagaacgttg
    14881 taaagaagcc attccagcaa atgcgccaat tttatatatg tatggtgctt tcgggaaacg
    14941 tctttcacgt accgattctg tcaatgaatt attcaaaaac cgtcgagcta ctgtatcgtt
    15001 aggctacatt ggattgtatg aagttgcttc agctttcttc ggtggcgaat gggaaacaaa
    15061 cccagaagca aaagctttca cacttgatat cgtcaaagaa ttaaaagcta atgcagatgc
    15121 atggggggat gaatatggtt atcatttcag tgtctattca actccaagtg aaagtctaac
    15181 tgaccgtttc tgccgtttgg atacagaaaa attcggcatc atcgaaaata tcacagataa
    15241 agaatattat acaaacagtt tccactatga tgtgcgtaaa aacccaacgc catttgaaaa
    15301 attggaattt gagaaggatt atccaaaata ttgttcaggt ggattcatcc actactgtga
    15361 ataccctatg ctccaacaaa atccgaaagc tttagaagct gtatgggatt ttgcatatga
    15421 caaaatcggg tatcttggta cgaatacgcc aatcgatcat tgctatcaat gtggtttcga
    15481 aggtgatttc aaaccgacag aacgagggtt cgaatgtcca gaatgtggaa atcatgatcc
    15541 aaaaacatgt gatgtagtca aacgtacttg tggctattta ggaaatccgc aagctcgtcc
    15601 gatggtacat ggccgccata aagaaatttc ctcacgtgtg aaacacatga aatgaggttg
    15661 tgaaaaagct gtcctgcatc aagtaataag aatagctaaa caaaaatagt tcctcatatt
    15721 tttcgtttag ttgggcttat tactgcagat gcaagctttt gaacaccgtt tattcagaat
    15781 aagaggctat gacaagtctt ttttcaccac cctctttcga tggctttgta tctttgaacg
    15841 ccctctatca aaataagaac aagctttctt gaacaatgga tttaggtcag tttgttctta
    15901 tttatgtggt cgagtagatt ttatcttcaa tagctatatc tttgaacctt aaatgaaaga
    15961 agttttttta tgagaaatcc aaaaccaaaa gaatggttag caaaagacta cagccaaaac
    16021 tatattgctg actacaaacc gtttaatttt gtcgatggtg aaggtgtccg caacagtctt
    16081 tacgttagtg gctgcttatt tgcctgcgaa ggctgcttca acaaagctgc acaaaatttt
    16141 cgatatggca aaccattcac acctgaatta gaagaacaaa tcatcgctga cttgcgcaac
    16201 gattacgtgc aaggattaac tttacttggt ggcgaacctt ttttgaatac agatgtttgt
    16261 ctgcaagtcg tacgacgtat ccgaaaagag ttcggtacga caaaagatat ctggtcatgg
    16321 acaggctata cctttgatga attgctacaa gattcagaag ataaattaga actcctttcg
    16381 caaatcgata tccttgttga cggacgcttt gaattaagca aacgtgacct aaaactacaa
    16441 tttcgcggaa gtagcaacca gcggatcatt gacgtccaaa aatctttaga atccaatcaa
    16501 gttgtgattt gggaaaaatg tacggatgca acagaaacct atgaacaaat caaaaagcaa
    16561 gacttgatat aaaaacagct ttgggagaga aaacggattt ccggtttcaa ctctcaaagc
    16621 tgtttttata tttcttgatt ttcccataat ggaaggacga tgttttcata agtcatcggg
    16681 tccagattcg tcaaagtaat ccctaacaat cggattcctt tttctactcc caagatttcc
    16741 tcccatataa ggcttgcttg ataaaagagt gcttcttttt tatagatata ttctggcaat
    16801 gtcactcttt tggtcacagt agaatagtcc gtgtacctga ccttcaaaac tactgttttc
    16861 ccatgttttt ggactcttct cagtgcttct tctacttttt cagctagttg gcgcaactga
    16921 gtcagcactg cttcttccgt ttgcaacggc tgcccataag tatgctcttt tcccacagat
    16981 tttcgctctc ttgtaacgtt tactggagaa tcatggattc ctctgacttt acgataaaga
    17041 gaatagccca tttttccaaa atttcgaatc aacatcattt ctgtgcattc atataggtct
    17101 tttcctgtat aaatccctag ctcatgcatt ctaggtacag ttttcttccc taccccatga
    17161 aatttttcaa ttggcaaagc ttttaaaaat ggcacagcgt cttgaggggc aactaccgtg
    17221 attcctctag gtttttgata gtctgatgct aatttggcca aaaacttatt gtaacttact
    17281 cccgcagaac atgttagctg gacactctcc caaatatctg cttggatcat tcgggcgatt
    17341 ttgatggctg acttgcaatt gattttattt tcggtcacat caagatacgc ttcatcgata
    17401 ctaactggtt ccacaatatc tgtataacga tagaatactt cccgtacttg tttggaaata
    17461 tcgctgtatt tatgatggtc gccaggctta aagatggctt gcggacaaag ctcatacgct
    17521 ttttgtgcac tcattgctga atgaattccg aattttcttg cttcataatt tgctgtagtc
    17581 actactccac gcccaccagt gtcactgggg tgtctggcaa ttactaatgg atgtccgcga
    17641 aactcaggat gatctcgttc ttcaaccgaa gcgaaaaaag catccatatc aatatgaatg
    17701 atttttcttg atgtatcatt atgtaaagga aattgcagct ccatcatcct cacctcttat
    17761 tcattataca agaacattcg ttcgcgttca agagatttga aaagttagta gctgatttcc
    17821 tcattagtct ttcattatag acaacttata ctatctcagt ctaattcatg gtactgacgg
    17881 tagacttctt gttttttcag cccatttttc agcgctactg ctttgatagc agcatttggt
    17941 ttttcgcctg aagcgatctt tgcttctact tgttccttta acgttcccaa ctcttcatca
    18001 acttccgtca ctggcatttc gaacaaattc ccttctacaa gcagacaaca ttcacccttt
    18061 actggatgtt cttcgatata agcgagtagc tcttccgtac ttcctctaag gtattcttca
    18121 tggacctttg tcaattcccg acacaaaacg acttggcgat ttccaaaaat ttctaagaaa
    18181 ttcgtcaatg tagcagtgat ccgataaggt gactcataaa aaatcagcgt aactgtctgt
    18241 tcttttagct ttttcaatgt ttctttctgt tcttttttct tacgtggaag gaagccataa
    18301 aataaaaatg gttgaggcac tagccctgaa gcaatcaaag ccgtcatccc agctgtaggt
    18361 cctgggatag agatcacaga aatcccttct tttatgcagg ctaaaactag ctcatgtcca
    18421 ggatcgctga tggaaggcat tcctgcgtca ctgacttgag caatcgtttt tccttctttt
    18481 aacaaagcaa tcaattgagg gacacgttct ttataattat gttcatgtaa acttttttgg
    18541 ggtgtatgga tttcaaaatg attcaataac ttttgtgtat tacgcgtatc ctcacttgcg
    18601 ataagatcgg attcttgcag catacgaaca cttctgtagc tcatatcttc taaatttcca
    18661 attggcgtcg gtacaagata taaatcccca tataccggat ttcccttgaa acttttttga
    18721 ttatacatct gtcacaatct ccttaataaa aagactggag cagtatttct ccgctccagt
    18781 ctcctcttgt tattggtcat cgaccgcttt cgcgatagat cacttctaga caaaacgcac
    18841 aaggctcgtc attgtccctt cttgatccat ataaatcgta gcagacatgg aatccttctt
    18901 cataaatttt ttcaagattc atacgagact tagaaagttc ctgcttcaca ttttcagctg
    18961 gagcttgtgc aacttggttc aattcccgca ggtgttcccg cagacgttga ttttccatct
    19021 ccaaagttgt atttttttct acgatctcat gcaaagcatg cttcatttca acaagttctg
    19081 ttaatgtatt tctcaactct gattctaatt gattcaatcc gtcgtataat gatctcttat
    19141 ccattcatct cacctacttg gataaattct ttcaattcat cccattcata ttcgacagga
    19201 ttttcccgtc cagtcagccg aatactaacg atacggctaa gcagattcaa ccctacgact
    19261 ttccctttac catcaggtgt agcaattact tttccaaagt cagggagttc ttttttcgct
    19321 gtctcgtatt cttcgctctc atattgcaga cagcacatca agcgtccgca taaaccggaa
    19381 atttttgttg gattcaaaga taaactttga tcttttgcca ttttgattga tacaggcacg
    19441 aaatcgccta aaaaagaaga acagcataat ggacgcccac atggaccaat tccgcctatt
    19501 atctttgctt catcgcggac accaatttgt cgaagctcaa tacgagtacg aaatacagcg
    19561 gccagatctt ttaccaattc tctaaagtca attcgcccgt ccgctgtgaa agaaaaaatc
    19621 aatttatttc gatcaaatgt gtattcggct ttgaccattt tcatttccaa ctcacgcaac
    19681 tgcacttttt cttttgcgat tttcaatgca tcataagcat cttttttgtt ttttttgtct
    19741 ttttctaatt cagcttgtga cgctttatgc aagatcggac ggatagcttc aggaaggtca
    19801 ttgggatcaa tctctttttt aggaaggaca acagtagcaa tatgtaagga ttgctgagat
    19861 tcgactaata ccttgtcact ataaacatat tcagagtctc ctggtgcaaa ataatagata
    19921 tgacctgcat ctctatatcg gacacctact acttctacca tttccatcct ccttgatcat
    19981 catcctttgc ggcaagctaa aaaatcgtat gcaaaacgaa ctgttccgca acagcctgaa
    20041 aactgacatt gctgcgtagt ttctgctctg cgatcaatat ccgttcaatt atctggttta
    20101 tctgtttcac tttgccatat tcgccgtttg ctactgcttg acgctgtgct tcctgataat
    20161 aaaacatcaa gacagataat cctgtaaact gttgtgtttt ttctttaaat gattttacca
    20221 attttttttg gacatagata aaggcttgag tatcttgctt ttgcaagtaa acgaaccatt
    20281 gataaatcaa gtccttagca tcattaaacc attcatcttg agagatttca actgcttttc
    20341 ctaaactgtt tgttaagaaa gccaaaagtt ttgctttttc caatggaatc tgctcttctt
    20401 gcaatcgatg gatcagtgct tctttcgaaa gttcttgaaa atgcagaact tgacaacgag
    20461 attgaatagt agggagaatt cttcctactg catctgtttc taacaccgct aaaaaatctc
    20521 cagggggctc ttccaagaat ttcaacaaac tgtttgccgc actgctattc attttttcag
    20581 cttctttgat caaaaaaact ttttttcttg attcgtaacc gctgcgactg aattccgtct
    20641 gcaaacgtct gatctgatcg actttgatgg tttgtccttc cggttcaata accaaaacat
    20701 ctggatgttc tttcttttcg atccgctgac aattgttgca ttttccacaa ggcagattgt
    20761 ctttcatttg ggtacagaac aggtgctgtg caagccatat acctacttca tgttttcctg
    20821 ttcctttctc cccttcaaaa agataagcat gagcgatacg cccatgctca aaactccgtt
    20881 gaagctgctg atacacgatc ggttgcattt gagccaagtt tatttcttga ttcatagctc
    20941 agctcctcct aaaattgatg gaatccttca acaggtaaga cgaagactgt ggcaccgcct
    21001 acttctactt ctactggata agggacgccc ccatccatcg aaatatccag tgtaacaggt
    21061 gtagatacaa attgctttct tgattcacac gtttttttga tgatttccaa tgcttcttct
    21121 acacggtcat cttcaatacc cacgataaac gtactgtttc cagccttaag gaagccgcct
    21181 gtcgatgata gttttgtggc acggatattt gcatcaatca attcatttga taaacggttg
    21241 ctgtctttgt cttggataat cgctaaaata attttcataa acgttcacct tcctagtttt
    21301 caaaaaattg cggatactgt tcaataattg tatgataact tgtttgcagc acttcttcaa
    21361 agctttttct tgcatcaact ttgtggattc tttccggatt ctcttctgcc agctttaagt
    21421 aagcatgacg tacacgctgg tggaattcca aaccttccga atctaaacgg tcgatttgat
    21481 tttgtctgtt tttcttgatt cttcttaatc cggtgtctga atctacgtca agataaaggg
    21541 taaaatccgg cgtagtacct tctgtagcaa actcattcaa ccgagcaatc tcggtaacgc
    21601 cgatgcgtcg gcctgcacct tgataagcaa gagaactgtc gacaaatcta tcgcataaaa
    21661 cgattttccc ttttgccaga gcaggcagaa ccttttcgac tagatgttgt cttcttgctg
    21721 ctgcataaag cagtgcttcc gtacgttcat ccatccgatc attttttgga tcaaggatca
    21781 ctgctcggat ctcttcagca atcggaatgc ctcctggttc tcttgtttga atgatttctg
    21841 tttttgctac ttctttcaaa agagggtaaa gttcattcaa gatgctggtt tttcctgcac
    21901 catcaggccc ctcgattgta ataaatagtc cgttcacatt gttgccccca tctcttgtgc
    21961 tgcttttata ttgtatctga aaaaacactt aattagaaat ttataactca cgtcttcctt
    22021 cgacagcttt caataaagta acttcatctg catactcgat atcagagcct acagacagac
    22081 catgtgctag acgagtcact ttgatacctg cgggtttgat cagacgtgag agatacatag
    22141 ctgttgcttc tccctctgtt gttgcatttg ttgcgataat cacttctttt acatctgcat
    22201 catgcagccg ctgcaacaga gaagaaatat tgatgtcttc tggacctgtc ccttccatcg
    22261 gtgataacac accatggaga acatgataaa gtccgtgata ttctcgcatt ttttccattg
    22321 ccattacatc tttcggctct tcgaccacta ggataacgct tcgatcacgt gaaggatttc
    22381 tacatatttc gcagggatct tcttctgtga tgtttccgca gatactacaa aaatgcaaat
    22441 cgcgttttac acttaacaaa gatttagcaa attcatttac tacttcatct ttcatatcga
    22501 tcgtataaaa ggcgagtctt gtcgctgttt tctgaccgat tcctggaagt ttcatgtagc
    22561 tgtcgatcag tttcgcaatt ggttctggat attgcatatt cattactcct tcagtttaaa
    22621 actaaaatcc tggcaaacct tttgcgtatt tccctaattt tttctcagtt tccacatcaa
    22681 tttgtgcgat cgcatcattg actgccatta cgacaagatc ctgaagcatc tcgatatcat
    22741 caggatcaac gacttcttca ttgatttgga tatctttcat gcgacgatcg cctgtaaaaa
    22801 ccgctttgac caattcattg gtggctttac ctgtaaactc tgttttattt agcagctctt
    22861 gtgcttctgc catttctttt tgcatttttt gtacttgttt catcatgcct tgcatattgc
    22921 ccattccacg catcataatt atctactcct ttttattaat catctaccac tgtaacagct
    22981 tcggagccaa acagttcctc tgcttttact acaagtgttt cttctggtac ttcttgttct
    23041 tgaggaagca actcgatttc tccttctgag tcttcagaag caaaagagtt ttctgttccc
    23101 tcttcttgtg cctgcgtcaa ataatttctt cgaagttctg gccagctttc tcttgtaata
    23161 taaacaggat caggcgcata atctttgatc attcggctta agttgttgtg taccgaaagc
    23221 tgcaattctt cgtcatttgc tgctctttga caaacaatct cataatcaaa cgcaatcact
    23281 agtcctgtag gtccagctgc aactggttcg cttgctttaa gcattgctcg ctgagtgaca
    23341 gatagactca tcagcaagtc ttcccatact tcttttactt ggttcagatg ctgtcttgtc
    23401 gcttcccgca gaactttata tactcgttcc gtcggtacac gataggtact tgtctgtttt
    23461 ttatgatttg tttgctgtac cggctgtact tttgtattct caattcctgt attactgcgt
    23521 agttccttga tttcgtttct tagttcctta atctcttgtt gtagctgttt tactatctga
    23581 tcatctgcaa atgctacatc tgctgaagga acagaagcag acggtagctg tgcaagtttg
    23641 actgtagcga cttccaaata aatagcggca ctattggtaa aacgaatttc attttgagta
    23701 tcgctcaaaa tctttatcat tgaaaacagc tgatccgctg aaacagtttg tgccaattct
    23761 gtaaatgtac ttctgatcgt actcgattgt tcctcaacta ggttaggtgc ttgctgatac
    23821 atcagcaagt cacggcagta ttgtaacata tcctctagaa agcgacgtga ttctttgccg
    23881 gctgcgagaa tttggttcaa tgtttccaat gcttctggta cttctttttt cacgcaagca
    23941 ctcaagtaac tgtccatcat ttcgtcagtt aagctacctg tcacttgcat ggcatcatct
    24001 aaagtaattg ttccttcact aaacgagatt gcctgatcaa gaatactcaa tgcatcccgc
    24061 atcccgcctt ctgcagaacg ggcaatcaca ttcaatgcag ctggttcata gctgatagtc
    24121 gattctttca agataaattc aagatgttcg acaatatctg ccgtattgat ccgtttaaaa
    24181 tcaaatcgct gagtccgtga aatgatcgta gctggtattt tgtgaggttc ggtcgttgct
    24241 aagatgaata tgacactttc ttttggctct tccaatgttt tcaacaaggc attgaaagct
    24301 cctgttgaaa gcatatgtac ctcatcaata atgtacactt tgtacgctgc ctttgtaggc
    24361 gcataatttg cccgatcacg gataaatcgg atttcttcga ctccgttatt gctggcagca
    24421 tcgatttcga tcacatcttc ttgcgtacca gcagtaattg atcgacacat ttcgcattcg
    24481 ttgcatggct caccgtcttt actgttcgga cagttgattg cttttgcgaa aatctttgct
    24541 gcactcgtct ttccggttcc tctgggtcct gtaaacaagt aggcatgtga ggttttgtgg
    24601 gaaacaatag cattttttag tgtctgtgtc acagcttttt gtccaacgat gtcttcgaaa
    24661 cgctgcgaac gccagactcg ataaagtgct tgatatgcca taaggatccc ccttttttaa
    24721 aaaattcact atctcttatt atacgtgata ttaggtaaaa aaactactat ccttctcggc
    24781 tttttcaggt atttaaacaa tacgcaattt tccatttcaa ttccgtctct agtccgagaa
    24841 gtttcacatc ttcctatggt ataataaata aacaataatt gtttggcact agctgaatca
    24901 gaggaggaat tttttatggg tcttatcaaa gcagcaacaa gtatggttgg cggcggtctg
    24961 gcagatcaat ggctagaagt catcgaacca gacaatatga gtgatacaac ggtcatgaca
    25021 aaaggtgtca aagtccgcaa agatgacaaa cgtggctcca atcgtaaagg aacagaagat
    25081 gttttaacag acggaacagt cgtccatgtc tatccaaaca tgatgatgct tttggtcgat
    25141 ggtggaaaaa tcatcgatta taccgctgag gaaggttatt acaccattaa aaatgacgca
    25201 gctccttcca tgttcaatgg aacactgaaa gaagcaattg ctgaaacttt tgaccgattc
    25261 aaatttggcg gcgtcacacc gcagaaacaa caagtttttt atatcaatct ccaagaaata
    25321 aagggaatca aattcggtac aagcgcgccg ttgaactact ttgacaattt ctataacgca
    25381 gaattattct tacgggcaca tggcacttat tctattcatg tcgttgaccc gattttattt
    25441 tatacaaacg caatccctaa aaacaaaaca caagtggaaa tcaatgatat caatgagcag
    25501 tatttagctg aattcctaac agcgctacaa tcagcaatca atcagatgtc tgcagatggt
    25561 caacggattt cttatgtacc atctaagagt ttggaattaa gcaaatacat ggatactgca
    25621 ttggatgatt cttggcgtga attacgtggg atggagattg tttctgtggc tgtagccagc
    25681 atttcttaca ccgatgattc tgtgaaactg atcaatatgc gtaacgaagg ggcgatgcta
    25741 ggtgatccta gcgtacgtga aggctacgtg caaggttcca ttgcacgtgg tatggaagca
    25801 gccggaaaaa atgaagcagg agcaatgacc ggctttatgg gtgtaggtat gggaatgaat
    25861 gcaaatggtt cttatctttc tcaagcagct caaaataacc aagaacaaat caaacaacaa
    25921 gcagaaaaac aaaatcaaca aactgcacaa ggaaatgcgg atacttggac atgtccagtt
    25981 tgcgggacag aaaattccgg aaaattctgt tccaactgcg gagctgccaa acctgtagaa
    26041 aacgttcagc ctaagctaca aatgcgatgc agtgaatgca atgagatcgt ggatctttcc
    26101 aacggcatcc ctaaattctg tccaaactgt ggtaaaccgt ttaaaggaat ccccgttaat
    26161 taatttattt agaaaggtgt gcagctgatg gatgttctta cacataaatg tcctaattgc
    26221 ggcggtccgt tgacttttga tcccaaagac caaaaatttc attgcgaata ctgcctgaat
    26281 atctatacag aagaagaagt cagccaatat gagcaaaagc aaaaagaagc gcgtatggct
    26341 ggcgaagaaa gcaaagaaga accttcacct accgaagatt ttacttttac agcccaagaa
    26401 cagcttgccg atatgaatga agccgagcgc aaagcggttg acgaggcagg cggtttttca
    26461 gatacagcgg acgaatctgc agataatacc gcagcgatag atctttttct ttgtcccaac
    26521 tgtggtgcag agatcgtgac tgatgcgaca actgctgcga cttattgtta ctactgccat
    26581 aatcccgtcg tactatcggg acgtttaagc ggagaattct tgccaaataa agtacttcct
    26641 tttgctgtcg agaaagaaga agcaatcaac agatttctcg cttggaccaa gaaaaaatgg
    26701 tttgttccca aagctttttt caataaggat caaatcgata agttgacagg tgtgtatttc
    26761 ccttattggg ctacagatgc tgaagtagat ggtgccatgc aggcgaatgg tacagttatc
    26821 cgcatatggc ggataggtga catcgaatac acagaaacga agcaatttgc tgtttcaaga
    26881 gcaggcaagc tttcttttaa agaactagta aaaaatgcct tatcaaaaaa tacgcaacaa
    26941 aagatggtcg aaggcgtgca acctttcccg ttagataaag caattgattt taaaagccag
    27001 tatttagccg ggttccaagc agaaaaaaga gatatcgaat atgaggcaat caaatcacag
    27061 attcaatctg aattaaaaga ctattcagag aagctgttaa aagataccgc aaatggatat
    27121 acaacattga ccggtgtgcg aacttctgct tcaatcacaa acgaagtaaa taattacgta
    27181 ttactgccgg tatggcttgt tacttatcga agcaatgact caagtaaaaa agtctactac
    27241 tacgcaatga atggacaaac aggaaaggtc agcggtgtcc tgccgatcag ccagaaaaaa
    27301 ttgggactga ccgctcttgg gattttcgct gggctagcga tcctctttct gatcggaggt
    27361 tacttgatat gatgaaaaaa ttttttttat tctttctttt gctagtaagt gttggctggc
    27421 cggtggctac tcatgcagat acagctacgg taaatgatga ggctgggtta ttcacaacag
    27481 atcaaatcca atctttggaa caacaggcag aaaagctgaa tgaaaaaatc aaaggacgag
    27541 tcttgatcgt aacgactaca tctaattcag aagaaccaag agactttgct gataattact
    27601 taagagacgc agtcggaaat gatcaaaatg gctcagtatt gctattagat atgggacagc
    27661 gtgaaatcta catttcgact tctggtaata tgatcgatta tttgactgat agcagaatcg
    27721 attcgatctt agatgatgtt tatgatcaga tgacaaatgc tgcctattac aatgctgcaa
    27781 aagcttacct gtctaaatca gcaacatatg tggaagacgg agttccagga ggacattatc
    27841 gtgtagacga agaaactggc aaaattacac gctataaggt tttgacgaca gtagaaatca
    27901 tcattgctgt tgttttggct cttatcctta gtcttgtctt ttattttgtg actgtttcgc
    27961 gttatcaatt gaaatcaggc atctacaaat atccattccg agaaaaatcc agtatcaaat
    28021 taactgaaaa aaccgatcgt ctgacaaatt cttttgtcac gacaagaaga atccccaaaa
    28081 gcccacctcc tggaagcggc ggtggcggaa gtactaccca ttctagcggt ggcgggactt
    28141 ttggcggtgg cggcagaagt ttctaaaaat tgctaaatga atgttcgcaa aaaaagggct
    28201 gtgacaaaaa tcgtgtcaca gccccttttg ttgcaaataa acggcgttca aaagcttaca
    28261 tctacggtga taagtccagc caagttaaaa acataagaga ctgtttttac ttggctcttc
    28321 ttattacttg atgcagaaca gctttttcac aacctcttgg taaacggtgt tctcctacca
    28381 actcctcggt cacaagtcac aatattcatc aaacatgaaa agctgttttt tgaaattgtt
    28441 ccttgtgact cagagttaaa cggcaggctc acaacctctt ggtaaacggt gttcaatcag
    28501 ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt tccgtttgtt
    28561 tctctaatac tcaggtcaaa acgctgattt cacaacctct ttgttcatca aaagataata
    28621 ttgtcttcta acaagtccat attcaaaata acgatatgat ggtctttaac ggtgataata
    28681 ccatcttttt tcagttgttt taaaattctg tttacactgc tagcagttga gatgccacaa
    28741 aatttggcga tttcttcatt cgtgatcgaa aaatcgatca gcatcccttc ttctgtccgc
    28801 acaccgaata aatcatataa ttcgtaaagt tgcgtacaaa cagctcccag ctttccgttt
    28861 aatagcattt gctgcatttt tttcatcgaa taaagcagac gatagcggta atactctttt
    28921 acatagtgtt gtagctttgg gtcttggttg atattttgcc aaaagcgcac acgattgatt
    28981 cgataaaact ctgcatattc tgattcaact cgaatattat acggcgaatc gatgaattgt
    29041 gaatattcgt cttttaaaag agagacgatt tcaagatctt tgatatagcg aaggttaaat
    29101 tctctcccat catttgaaat aacacttgct tttaccgttc cctctttcaa gatatacgta
    29161 tgggaatctt ccatcccttc gaatgtcaaa tatttatgat atcctttgcg tataatcgga
    29221 aaatcatttt tatccaaata ttcctttaag actctactat ccaatgatgc tcccccatcc
    29281 aaactttcat ctaaaaagat catacagtac tttaaaactt tttaaagtcc aaaggtattt
    29341 tactaatgat attaacattt gttaagtacc tttttccatc acttatgcga tttttttcct
    29401 tttttttatt cgttatactg cagaagtaaa attttttaag aatagaggga aaaaacatga
    29461 attactggat gaacacaatc attaatcgat tagaaacagc atatcaaaca agatttgata
    29521 tgaaagcttc tttagtcttt ttaaatgatg cgtatcaaaa ctcgatcgaa ttgatcaaag
    29581 cagtggatga gaatccaaca aacgaatgcg aagaattttt aaatctgttt atgtctacaa
    29641 gagatttatt tattcgccaa cttgttgatc gctatccatc caattatcac gatgtagaag
    29701 ttcaaataca aaaattaaaa gcatattcag cttaagggga atgtataaca tacatggaaa
    29761 agaaattatt taataaatat tttattggaa tcacattgat caattttatt gtctacctga
    29821 tttattatct gttgatggtc atcatggcag tcattgcgca agacacctta cacgccagct
    29881 tgagccaagc aggttttgct tctgggatct atatcatcgg aacattgctg gcacgcttat
    29941 tcatggggaa acagttagaa ctatttggtc ggaagtttac cttaagaggc ggcgcgatct
    30001 tctatctcct ttcaacatta gcatatcttt atacgccaac tatcggggca ttgtatttag
    30061 ttcgattctt gaatggattc ggctacggta cagtttctac agctacaaat gctatcgtta
    30121 ctgcatatat ccctgcatct aaaaaagggg aaggcatcaa ttattatgga ttaagtacta
    30181 gtttagctgc cgctgttggg ccatttttag gaatgatttt gctgaatatg actgattttc
    30241 gtttcattat ctggttttca attgtactgg tcttctttgt tacgattgcc tgccttgtct
    30301 ttcctgtaaa aaatattcca ttatctgaag aacaaaaaat atctcttcgc tcatggaata
    30361 tcaatagttt tattgaaaag aaagcacttt tcattacagg aattgccttt ttgatgggtc
    30421 tttcttattc aagtgtttta tcatttttat cttcttatac aaaagtaatc cacttagttg
    30481 ctgctgggtc atttttcttc gtcgtatatg ctttggtcat cacatttaca cgtccattga
    30541 ctggaaggat tttcgatgtc aaaggagaac aatacgtgat gtatcctagc tatttattct
    30601 taacagcagg attattttta ctgagtgtaa cgacgaatag tgtgacattg cttatctctg
    30661 gagcattagt cggcctaggt tatggtacgt ttatgtcaaa tggacaggct gtttgtttaa
    30721 aaatcgtccg cgaacatcga atcagtattg ctttatctac ctactttatt ggtttagacc
    30781 taggcttagg cgttggtcct tatttgatgg ggatgctaaa atctcttact tcatttagag
    30841 gattgtatgt gattgccgga gtcattccat tgatatgtac agcactctat cttgttaatg
    30901 gccgaaaaca tagtcatgaa gcagagggcg aattgattga atcattgaaa aatgaaattt
    30961 gatcttgatg aatagataaa aaaaaccgtt cagatctgaa aataatctga gcggtttttt
    31021 gagtaaaaat agtttatgat tattcttaat taaacatcta tttattttta aagctttcat
    31081 ttatcgataa gaatagttta actgttgatt tattccattt tccgttcatc tggtacgatg
    31141 ccttgactta gcaaccactt gtggaagaac cattctgcca caccgatcaa tagagcagga
    31201 atcaatactt tcatccaaat atcgactgta tcattcataa aatatagata gagccacaag
    31261 ataactgcag ctgaaccagc atctgcgatc gtcgctgcga tgttccctac tttacgtaag
    31321 atcaacagat cccctagcac atagatcatg actacaataa ttaccccaac aatcgttgaa
    31381 tccataaagc taacaccgta taaacctgtc agcacgaccc acagaatcgc ccagatcatg
    31441 attgccttca ctaccaacgc cttcacatgt ttcattttct gcactccttt ctcctggata
    31501 aaaaagtaag attatctaac aagataacct tttcattatt ataccatcgg ctgagagaag
    31561 cgagcaaaga taaggtccta tctaaaaatg aggaagccac ttagaacatt cacgtaataa
    31621 tttatccgta cagacattcg atatggtgtt cctttacttt cgcatactgt tcttttgtca
    31681 gttctttact tgtcaccaga taatcgattt catgaaaagc aatggcttga tgcatgaatt
    31741 tatgtgcaat tttttgctgt gtaactaaaa cggcaagccg gtctgattgt tttctaaaag
    31801 cttttttgac cattgcctct ccttcatgag tttctgttac tccttggctg aaatctagcc
    31861 cgcgacaact cattatcgct aaatccgcat aatatgacaa tgcatcgtta taggcttttg
    31921 aaccgttgat tgttgctcgt ttcggaacaa ttgcccctcc caatacagat acttttgccg
    31981 ttgtatattc actcaacata gcagctgtga cgatgccatt cgtaatgatc tgcaaaccat
    32041 caaatcttgc taggtttctg actaagtgta aagaggttgt actggaatcc aagaataaga
    32101 ccatatcatc tcgaatcaaa gaagaagctg tatctgcaat cttacgtttt tctgtttctt
    32161 cttctaattt ggtgatcttc atcaggtaag gcacgtcaat ttttcgattg tcaataagca
    32221 tggcgccacc gcgaactcta cggatcaatc cacttttttc cagaaaaaca aggtcacgtt
    32281 taatcgtggc ttcgctataa tttactaact cgatcaaatc ggtgactctt gcaaattggt
    32341 tgttttttag ataattcaca atctcctgct gtctattctc cacttcattc aacataaaat
    32401 caccccattt actttatata gtaacaggaa aaaagaggtt gtgacagaag tgattggctt
    32461 cgagaaataa gaagaaattt ttcgaaaaaa ctacttctga acatcgtgtg tggttaagag
    32521 ggtatgacaa gactattgtc ataccctctt gaccacacat ttttattttt agtataaact
    32581 agccttgcct actgtgttca aaatttccat tttatgtttg accacttctt gtaccttttt
    32641 attagctgct ggataaattg cattcggctc gtacatcgat ggattttcac tcaatatttc
    32701 tctcaaagca ttgaaaaaga cgcttttaag atctgagctc aaattcactt tggcaactcc
    32761 gtattttact gcttcgctga cttctgcgtc cggattacct gaaccgccat gtaaaacaaa
    32821 tggaagatcg atttgttggt tcaattcttt cagtaagtcg atatttaatt ttggagttct
    32881 gtttttcgga tacaagccat gcgctgttcc aattgctaca gcaagtgtat cgattcctgt
    32941 ttctttcaca aaaatcttcg cttgatcagg atctgtataa atgattgttt cagcacctcc
    33001 ttcagcagaa ccattattcc caatcgttcc aagctcagcc tccactgaga catttaccgc
    33061 atgagctaac gcaaccactt ttttcgtaat ctgtacattg tcgtcgaatg atttcaaaga
    33121 cgcatcgatc atcacggaag aatagccgtt tcgaatcgcc cgtaagacat cttctatcgt
    33181 accgccatga tccaagtgga taacgatcgg cagtttactt gtataagcat aggctcttac
    33241 tgccgcaaca aactcatcac ttaaataggc gatttcatca ggatgaattt ctaagattac
    33301 tggtgcttct aatctctctg cttcttcgat gattgctttc atcatatcat agctgcaaat
    33361 attgaaggct ggtactgcaa attgattctc tttagccact ttcaataact ctttcattgt
    33421 atataacatt acgcttccac ctcatttgtt agattttcat ttttcgtcca cttgtcttgt
    33481 tttttctctt tcactgtttt tttcaatagt ccaagcataa aggctgtagt gactgtacct
    33541 gatgcaattg ccaagacata cataggtaaa ttgttcataa catttggaat tagcatcacc
    33601 caaatccctc catgaggtgc cattgaagta actcctactg ccatggaaat ccctgcagcg
    33661 actgctgaac ctgtcatcaa actaggtatc acgcggatcg ggtcagatac cgcaaacggt
    33721 atagcacctt ctgtaatata cgaagcgcct aaaatccagc atgattgacc tgaagtccgt
    33781 tcttctttgg taaatttatg tttgaataat aaagtggcta aagcaatacc taggggaggt
    33841 gtcattcctg cagccataca agcagcaatc ggtgcattta ctccagctga catcaaaccg
    33901 atcgagaaag tcgaagtcga tttattgatc ggcccaccca tatcagctgc catcatgatc
    33961 ccgataagta atccgaacaa tagcccggat gtatttccca agctattcag ccaatttgtc
    34021 aaagcatcca acacccatgt gatcggttgt tcaatcacaa ataccatcaa aagcccaata
    34081 attaagatcg ataaaaaggg gacgacaatc agctgataaa tagcagaaaa ttcttttttt
    34141 acgctaattt tatttgccag aaaatcacag atataccctg ctagcacgcc accaatcatt
    34201 ccgccaagaa aacctgaacc acctgtagag gccagtaatc cggcaacaat tcctggggca
    34261 aaacctgctt ttccagcaat cgatgtagca atccccgcgc ctaacgcagg aaccatcatg
    34321 gcaaacgctg tatctcctcc gatccttgaa aatgcagctg ctaacacatt atagtctgct
    34381 gagttgggat cagccgctgt aatcccaaag gcaaaactca gtgcgatgat gattccgcct
    34441 gcgatcacaa atggaagcat gtaattcaca ccattcatga aatgattata gattgtcggc
    34501 tttttctcat ttgcagctgg ttctttttca ttagacacaa cgaatacgcc aatatctctc
    34561 aatgcttcgt ctattactac ttgcggttct ttgattgctc ggcttgtaga gacacgcttt
    34621 acttttttcc cggcaaatcg gtccaattcg atttctttat ctactgcaag aatgattgca
    34681 tctgcctctt tgatttcttg ttcagataac tgattttctg tttttacacc ttgtgtttcg
    34741 aattttgctt gatggtttag atttcttgct gttacttcta atttttctgc cgacatataa
    34801 gtatgagcaa ttccagtcgg gcaagcagtt acaccaacga ttttcatgat tattttcctc
    34861 ctttttcttc actatcgata ctcggacaaa gactctacta ttttcattct tgactaatct
    34921 gtctgatgat atctgctggc gtagtggctt cttttattcc ctttctaaaa tctgcattca
    34981 tcaatcgtct ggagagtgtg ctgagaatct ctagatgcat atctgctgat ttttcaggaa
    35041 ctacgatggc aaaaatatat tcaattggca tttcatctat cccgctatag tccgttaatt
    35101 tggctgtatg aaaaaaacac agtgctggtt ttgtcacata tttgcttttt gcatggggaa
    35161 tcccaaatcc atcttctatt cctgtggaga tctcctcctc tcgtttgaat agtgccttaa
    35221 cgactttttg aacatttctt accttatttg tttttttcaa cacatcccct attcgctcaa
    35281 ataatgatcc ttttgaatct atcgatggat cgaaaacaat cagttcttct gcgatcaatt
    35341 gatcaaacct cataaaaaac ctctccccca caaaaaatgt taacgttttc ttggaactga
    35401 ggtcagtata gcatcgctag ctgtaccgtt caatattatg aatgaacaga ggaaaaagac
    35461 ccatcatccg cgtgtataag tcattaaaat gaacgatcta gctcacaaac aatcgcaagt
    35521 ctataacaaa acaaaaagcc ttccttgaaa ggaaagctta gcttctaatt ttaggtaaat
    35581 ggttttttac ttcgaaataa cccctcttca tttcgttaat ataaggtgtt tttatcaagt
    35641 ggataattaa aactatatac tagtttttca ttgttttgtg cattatgatc ctacgacttt
    35701 ttcagcatca aacgaagttt taaattggca aaaaacataa aaaaacagac tatccctatc
    35761 agatagtctg ctaaacgttg agttatcagc gtttttatta ttccgcagcg tttgcgtctt
    35821 tgagaccgta ttttttgttg aaacggtcca cacgtccgtc tgcttgtgtg aatttttgac
    35881 gtccagtgta gaatggatgt gagtcagaag ttacttcgac acggatgact gggtatgtat
    35941 taccgtcttc ccattcaact gtttcttgtg aagtttttgt tgaacctgac aagaatttga
    36001 aaccagttgt tgaatccatg aatactactg ggtggtaatc tggatggatg ttttctttca
    36061 tgattttttc gctcctttgc cctgctccat ttgctgaacc agagttgatt tgtcgattta
    36121 caacattaac atcttaacat gtttcagtca aggttgcaat tatcttttca gatttctggt
    36181 cggatttttt ttaccaaagg agacatcttg aaattctttg aaaaattgct gattgttttt
    36241 tgttttacgc aaaaagcgga tcagttgttc cgtatattct aatgaatctc cagtcatatt
    36301 ttttcgaagt ttccaaattt cttctaattc ttcacttggc atcaataatt cttcttttcg
    36361 ggtacctgat tttttgatat cgatggctgg gaagatccga cgttcagata attcacgtga
    36421 aagttgtaat tctagatttc cagttccttt gaattcttca tagatcacgt catccatacg
    36481 gcttcctgta tcgactaaag cggtcgctaa aatcgtcaaa ctgccacctt cttcgatgtt
    36541 tcttgctgcc ccgaaaaatt tcttaggttt atacaatgcg gctgggtcaa tcccgccact
    36601 caatgtacgt ccgcttggtg gaacgactaa gttataggcc cgagcaagtc tagtaatact
    36661 atccatcaag ataacgacat ctcgtttatc ttcgactaaa cgcattgcgc gttccaatac
    36721 taattcagaa actcgagtat ggttttgcgg ctgcagatca aatgtagaag ataccacgtc
    36781 gcctttcacg cttcgttcta gatccgttac ttcttccggt cgttcatcaa tcagtagaac
    36841 gattaattct acttccggat agttttcggt gataccattt gcgatttctt ttaatacaga
    36901 agttttccct gcttttggcg gtgcgacgat caatccacgc tgaccaaaac cgattggaga
    36961 aaagatatcg atcattcggg tagatagtct ttgcggtgtt gtttccagtt gaagctgtct
    37021 ttgcggataa agaggtgtta aggctggaaa atgcggacgt tcttttgctt cttccggatc
    37081 ttttccatta acgctttcca cgtgcatcaa accataataa cgttccgatt ctttaggtgg
    37141 acgggctttt cctgccactt tgtccccatt tctcaaactg aatcgtctga tttgtgaggc
    37201 agaaatatag atatcttctg cacttggtcc ataattgatt ggccgtaaaa aaccgtaacc
    37261 atcttgacca acgatatcca atattccttc catataataa aatccttgtt tctctgcttg
    37321 tgcacgaatc acagctagag ataattcctt tttattcatc ttactgtaat aaggaatttt
    37381 aaattctttg gcataactat aaacttcttt taacgttttg ttctctagtt ctgccattgt
    37441 caaataatcg ctcattcagc tcccacttcc tctatcattt caattgatgc ccctaaggct
    37501 gttagtttct cgataatatg atcataaccg cgtaagatat aatctacatt gtaaatcgtc
    37561 gttgtccctt ctgccaatag tccagcaatc actaaacaag cacctgcccg aagatcagaa
    37621 gccacgactt ctgtgccgtg aagtttattt ggaccattca cgatgatcat atttccttcc
    37681 acggtaatat cagcgcccat tcgtgctagt tccggcacat ggttcacacg tttcgcatag
    37741 atcgtatcga tgatttctga agtgcctgta gtcatcaata gtaacgcagt aagtggctgc
    37801 tgcagatctg tggcaaatcc tggataagga taggtcatga tgtttgcggg ttttaattct
    37861 ttcgaggggt aaacttcgat ttcatcttcg ctgattttca tatggacacc aatttcctgc
    37921 aattttgcta taaagctttc caaatgttca aagatcacat ttttcacttt gataccattt
    37981 cctactgcag ctgctaatgc tagataggtt cctgcttcga tgcggtcagg aatcatgaaa
    38041 tgccgacagc catgtaatgt ttcgactcct tcgatacgga tcacatctgt acccgctcct
    38101 cgaacttttg cccccatatt attcagtaaa gttgccacat ctatgatttc cggttctcgt
    38161 gcagcgtttt caattactgt ctgtcctttt gcttttacag cagctaacat cacattgatc
    38221 gttgctccga ctgaaaccac gtccatgaaa atccgattgc cacgcaatcc tttattttct
    38281 gtccgtaaat acatggcacc atgttcattt gtcacttcag cacctagagc ttcaaatcct
    38341 ttgacgtgta ggtcaatcgg tctagggcct aaataacagc cacctggcag gcctactacc
    38401 gcttcgccaa atttaccaag cagtgttccc ataaaataat aagaagcacg caaactattg
    38461 atttttccgt gcggcatcgg aatcgatacg acttctcgag gatcgatttc tagtatgttg
    38521 ttggaaaaat ggacagttgc gcccatgatc tctaaaattt caattaatga atgaacatct
    38581 tgtatgtccg gcacaccttc caagatcact ggggaatcag ctaagattgc agctggaatc
    38641 aatgcgactg cactattttt tgcaccgctg atcgttactt caccagctaa aggtcggttt
    38701 cctttgatta cgattttttt catctgtttc caccttgttt attaatcttt tttcatggat
    38761 atgcttgtat tttatcatag ataaaaaaaa agaaacaagc atggtctgcc gttttaagta
    38821 aaaaagaatg gagagcgact tcaaaagtcg ttctccattc ctatatatcg tatctgttat
    38881 gtggatgctt acgctttgtt agctgaaccg aaccagtcga tatgagattt agcatcttca
    38941 acgattgctg ctttacctgg agctaataat ttacgagggt cgaatccttt accttcttta
    39001 tctttaccag cttcgatata tttacgagta gctgcagcaa atgataattg gaattcagtg
    39061 ttaacattta cttttgagat acccattgag atagcttttt cgatttgatc ttgaggaata
    39121 cctgatccgc catgtaatac taaaggcatg tctggaccta cagcatcagc aattgcttgt
    39181 aagtggtcaa aagctaatcc tttccagttt tctggatatt gaccatggat gttaccgata
    39241 ccgcaagcta agtagtcgat acctgtagca accatttgtt tgcattcttc aatatcagct
    39301 aattcgccgt taccgatgat tccgtcttct tctccgccga ttgaaccaac ttcacattca
    39361 actgagatac cttttgcatg tgctttttca acaacgtctt tagctaattt caagttttct
    39421 tcaaatggta gatgtgaacc atcgaacatt actgaagtat agcctaattc gatacattcc
    39481 aaagcagctt cgtaatcacc atggtctaag tggatagcaa ctggaacagt gatgttcatt
    39541 gcttcaacta aatcagtgat taaatcttta cagattttgt aaccacccat gtatttagct
    39601 gcacccattg atgtttggat aagtactggt gcttgttttg cttcagccgc ttcaaggatt
    39661 gcttgagtcc attccaagtt gtttgtgttg aatccgccga cagcgtaacc gcctttacga
    39721 gcagctttta aaaattcagc tcctgatact actggcataa taaaattcct cctaagtttt
    39781 gtaaaactaa atttgttaag acaattctta accaacgacc ctattttacc aaacatttcg
    39841 gtagttttct actaaaatca atgaaaatga aaattttcaa gaagtttttt gcaaaaaata
    39901 aaatatccaa tagcgcttta atcgaaaaaa gtcttttgct ttctatcttt tttcctacaa
    39961 ataataacaa atcagaaaaa gagaaaagtg acaagaaaat tgcttatccc cttctcttgc
    40021 tacttttctc tttctatcaa gttcaaaaat tattttgttt cgtgatctgc taatgatgct
    40081 ccgacaaatg ctttgattaa tttttgcggg cgatttggac gagaaatcaa ttctgggtgg
    40141 aattggcatc ccacaaagaa ttttttctct ggcagctcta cgatttctac taaacggtta
    40201 tctggtgaga cacctgaaaa gactagccct tgttcttcaa acaattgacg atatttgtta
    40261 ttgaactcat agcggtgacg gtgacgttct tgcacaacat cggctccatc atatgcttca
    40321 gccgtcttcg ttcctttttt gattttacat gggtataatc caaggcgtaa agttccaccc
    40381 aaattctcga tgttttcctg atcagccatt aaatcaataa tattgtttgc tgtatctgga
    40441 ttcgtttcag ctgatgacgc atcttctaat ccaacgacgt tacgtgcaaa ttcgacacaa
    40501 gccatctgca taccaagaca aatacctaaa aacggaacgt cattttcgcg agcataacga
    40561 atcgcttcga ttttcccttc tactccgcga tcaccaaatc caccaggaac caagattcca
    40621 tctgcgtctt tcaaacgttc ttctacattt tctctcgtta attcttgtgc tttgatccaa
    40681 tcgatctcga tgtctgaatt ataggcgaaa ccagaatgtt tcaatgcttc aactactgag
    40741 atatatgcat ctggtaactc gacatattta ccgacaagcg caatccgtac ttttttcttc
    40801 aagttcaaga cacgttcttc taattctttc cattctgtca tgtctgcttc tggtgtatct
    40861 agttttaaat ggtcgcaaac aatttgatcc attccttgtg cttgtaaaac taatgggatg
    40921 gagtaaagtg tatctacatc tggcgactcg ataactgctt ctggttcgac atcacaaaat
    40981 tgtgccaatt tgtttttcac gctttgagaa acaggttgct ctgtacgtac caccaaaata
    41041 ttcggttgga tacctaaacc acgtagctct tttacactgt gctgcgttgg tttagttttc
    41101 atttctccag cagctttcaa gtaaggaatc aatgttgtat ggatatacat aacattatct
    41161 gtaccgacat ctgctttcat ttgacgaagt gcttcaagga atggcaaaga ttctatatcc
    41221 cctactgttc cccctacttc tgtaatgatc acatctgaat cagtcatttt agctgctcgc
    41281 atgatttttt ctttgatctc atttgtaata tgaggaataa cttgaacagt tgctcccaag
    41341 tattcgccct tacgttcttt acgcaaaact tcagaataga tttttcctgt cgtcacgttg
    41401 gagtatttgt tcaaattgat gtcaataaaa cgttcgtagt ggcccagatc caaatctgtt
    41461 tcagcaccat catctgttac gaatacttct ccgtgctgat atgggctcat cgtccccgga
    41521 tccacgttga tgtatggatc aaatttttgg atagttactt ttaagccgcg gtttttcaat
    41581 aagcgtccta aagaagctgc aacaatccct tttccgattg atgaaacgac accgccggtc
    41641 acaaaaatat attttgtcat aagttcaaaa actccttttt aatttatgtg caggaaaagt
    41701 ctcagacaat aaacaaagct ccctattctt caataagaat agggagccga aatttctgta
    41761 aacttctgac cctctaaaag ggtgcccaag gtgtataata caagctgact aagggaaagt
    41821 caagtactca aatgaatact atccgcttac aattttttct ttttcgatat cctttgagaa
    41881 attatttctt aagtccttta tgagtgcaaa aaaaatttaa cccacataat aagcttgata
    41941 taaacatagc taacgttaaa cctaagcaaa tcagaaaatc gttactgaaa aattttaagt
    42001 gaaataatag ataaaaaaat aaaacaaaca ttatgataga taatatccca cctaacaaag
    42061 aactactttt tcctacttgc agcaacagtt tatatagctt atcgtagaaa caatcttttt
    42121 ctttcaccct tatttacctc ctttacgtaa aaaacgtagt ccacagttct cggatttttg
    42181 gcatatcttt tctattaaat aatatcaaaa acaaaacaat tccactaatt ttttcaaaga
    42241 tgaccttttt ttcaacaaaa gtgccttgta gggattcatt gaaggtaacg aatcacataa
    42301 gtaaaaaata aggagatgcg acaaaagtcg tgcctcctta tttctaaata aacggtgttc
    42361 tcctgccaac tcctcggtca caagtcacaa tatccatcaa acatgagaag ctgtttttga
    42421 aattatttct tgtgattcgg agttaaatgg taggctcaca acctctggat aaacggtatt
    42481 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca
    42541 caaaattttt cttaatactc gaaactaaac gtctttttca aaacctcttg gtaaacggtg
    42601 ttcaaaagct tgcatctgcg gtaataagtt cagccaattc aaaaacaaga aaagctgttt
    42661 ttgcttggct attcttatta cttgttgcag ggcagctttt tcacaacctc tccactaaac
    42721 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
    42781 ttttccgtta gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac
    42841 ggtattcctg ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg
    42901 tctgaatgcc gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc
    42961 taatgcgtca atcattacat tatccttacg caattctttg atagcattat ggatagttga
    43021 aggtaagtcc tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg
    43081 gtcaacagct gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa
    43141 tactgccatt gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt
    43201 ggataaaccg cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc
    43261 gacataaaca ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt
    43321 gcaaacggct gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt
    43381 ttcacttagt tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa
    43441 agacatattg caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt
    43501 cgcgtgaagt ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt
    43561 atcacaagca tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac
    43621 ttcatgatgt gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg
    43681 gcggcaattt tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa
    43741 ggtcgttgtg atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc
    43801 taaattgaaa gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc
    43861 gcgaggatcg ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc
    43921 aacttttcca tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa
    43981 atacatatcg ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat
    44041 tttattttct aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc
    44101 taagatgtct gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt
    44161 gatttcttca cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat
    44221 tcagtcaaag aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc
    44281 accttgcgcc aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt
    44341 cttgtttttt gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc
    44401 catattcaat ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag
    44461 cgaatacata cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata
    44521 atagcgaatt tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac
    44581 ggacatagat cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt
    44641 ggctaaagtg taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat
    44701 gagatctcta ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg
    44761 attttcgtac cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt
    44821 tatt
//
LOCUS       DANKMN010000013        43766 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000013 DANKMN010000000
VERSION     DANKMN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            172..516
                     /locus_tag="PW224_000719"
     CDS             172..516
                     /locus_tag="PW224_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL2907430.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="PW224_000720"
     CDS             517..936
                     /locus_tag="PW224_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL2907431.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1028..1378)
                     /locus_tag="PW224_000721"
     CDS             complement(1028..1378)
                     /locus_tag="PW224_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907432.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="PW224_000722"
     CDS             complement(1344..2675)
                     /locus_tag="PW224_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL2907433.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="PW224_000723"
     CDS             2931..3497
                     /locus_tag="PW224_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2907434.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="PW224_000724"
     tRNA            3642..3729
                     /locus_tag="PW224_000724"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            complement(3781..4986)
                     /locus_tag="PW224_000725"
     CDS             complement(3781..4986)
                     /locus_tag="PW224_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2907435.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5124..6012)
                     /locus_tag="PW224_000726"
                     /pseudo
     CDS             complement(5124..6012)
                     /locus_tag="PW224_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(6099..6299)
                     /locus_tag="PW224_000727"
     CDS             complement(6099..6299)
                     /locus_tag="PW224_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907436.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(6328..6750)
                     /locus_tag="PW224_000728"
     CDS             complement(6328..6750)
                     /locus_tag="PW224_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2907437.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            complement(6767..7189)
                     /locus_tag="PW224_000729"
     CDS             complement(6767..7189)
                     /locus_tag="PW224_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2907438.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            7480..7671
                     /locus_tag="PW224_000730"
     CDS             7480..7671
                     /locus_tag="PW224_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907439.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            7682..7948
                     /locus_tag="PW224_000731"
     CDS             7682..7948
                     /locus_tag="PW224_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907440.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(8189..8668)
                     /locus_tag="PW224_000732"
     CDS             complement(8189..8668)
                     /locus_tag="PW224_000732"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907441.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            8713..9420
                     /locus_tag="PW224_000733"
     CDS             8713..9420
                     /locus_tag="PW224_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL2907442.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            9599..9940
                     /locus_tag="PW224_000734"
     CDS             9599..9940
                     /locus_tag="PW224_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907443.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            9933..10679
                     /locus_tag="PW224_000735"
     CDS             9933..10679
                     /locus_tag="PW224_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2907444.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            10685..11371
                     /locus_tag="PW224_000736"
     CDS             10685..11371
                     /locus_tag="PW224_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907445.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            11378..12211
                     /locus_tag="PW224_000737"
     CDS             11378..12211
                     /locus_tag="PW224_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL2907446.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            12228..13079
                     /locus_tag="PW224_000738"
     CDS             12228..13079
                     /locus_tag="PW224_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2907447.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            13076..13435
                     /locus_tag="PW224_000739"
     CDS             13076..13435
                     /locus_tag="PW224_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL2907448.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            13450..13611
                     /locus_tag="PW224_000740"
     CDS             13450..13611
                     /locus_tag="PW224_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2907449.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            13614..13913
                     /locus_tag="PW224_000741"
     CDS             13614..13913
                     /locus_tag="PW224_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907450.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            13913..14227
                     /locus_tag="PW224_000742"
     CDS             13913..14227
                     /locus_tag="PW224_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL2907451.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            14404..14715
                     /locus_tag="PW224_000743"
     CDS             14404..14715
                     /locus_tag="PW224_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2907452.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            14712..14930
                     /locus_tag="PW224_000744"
     CDS             14712..14930
                     /locus_tag="PW224_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HDL2907453.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            14943..15224
                     /locus_tag="PW224_000745"
     CDS             14943..15224
                     /locus_tag="PW224_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907454.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            15221..15547
                     /locus_tag="PW224_000746"
     CDS             15221..15547
                     /locus_tag="PW224_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907455.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            15544..15741
                     /locus_tag="PW224_000747"
     CDS             15544..15741
                     /locus_tag="PW224_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907456.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            15738..15947
                     /locus_tag="PW224_000748"
     CDS             15738..15947
                     /locus_tag="PW224_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907457.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            15944..16231
                     /locus_tag="PW224_000749"
     CDS             15944..16231
                     /locus_tag="PW224_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907458.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            16823..17290
                     /locus_tag="PW224_000750"
     CDS             16823..17290
                     /locus_tag="PW224_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HDL2907459.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            17589..17930
                     /locus_tag="PW224_000751"
     CDS             17589..17930
                     /locus_tag="PW224_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907460.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(17923..18114)
                     /locus_tag="PW224_000752"
     CDS             complement(17923..18114)
                     /locus_tag="PW224_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDL2907461.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDVTK"
     gene            18464..18676
                     /locus_tag="PW224_000753"
     CDS             18464..18676
                     /locus_tag="PW224_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907462.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            18683..18907
                     /locus_tag="PW224_000754"
     CDS             18683..18907
                     /locus_tag="PW224_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907463.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            18934..19467
                     /locus_tag="PW224_000755"
     CDS             18934..19467
                     /locus_tag="PW224_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL2907464.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            19454..20698
                     /locus_tag="PW224_000756"
     CDS             19454..20698
                     /locus_tag="PW224_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDL2907465.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            20711..22210
                     /locus_tag="PW224_000757"
     CDS             20711..22210
                     /locus_tag="PW224_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2907466.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            22215..23141
                     /locus_tag="PW224_000758"
     CDS             22215..23141
                     /locus_tag="PW224_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL2907467.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            23268..23870
                     /locus_tag="PW224_000759"
     CDS             23268..23870
                     /locus_tag="PW224_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDL2907468.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            23883..24806
                     /locus_tag="PW224_000760"
     CDS             23883..24806
                     /locus_tag="PW224_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2907469.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            24824..25087
                     /locus_tag="PW224_000761"
     CDS             24824..25087
                     /locus_tag="PW224_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907470.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            25099..25428
                     /locus_tag="PW224_000762"
     CDS             25099..25428
                     /locus_tag="PW224_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDL2907471.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            25425..25751
                     /locus_tag="PW224_000763"
     CDS             25425..25751
                     /locus_tag="PW224_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907472.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            25735..26073
                     /locus_tag="PW224_000764"
     CDS             25735..26073
                     /locus_tag="PW224_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2907473.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            26073..26465
                     /locus_tag="PW224_000765"
     CDS             26073..26465
                     /locus_tag="PW224_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HDL2907474.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            26476..26988
                     /locus_tag="PW224_000766"
     CDS             26476..26988
                     /locus_tag="PW224_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HDL2907475.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            27034..27402
                     /locus_tag="PW224_000767"
     CDS             27034..27402
                     /locus_tag="PW224_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HDL2907476.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            27456..27743
                     /locus_tag="PW224_000768"
     CDS             27456..27743
                     /locus_tag="PW224_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907477.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            27760..30879
                     /locus_tag="PW224_000769"
     CDS             27760..30879
                     /locus_tag="PW224_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2907478.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            30912..31679
                     /locus_tag="PW224_000770"
     CDS             30912..31679
                     /locus_tag="PW224_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDL2907479.1"
                     /translation="MYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            31676..34507
                     /locus_tag="PW224_000771"
     CDS             31676..34507
                     /locus_tag="PW224_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2907480.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            34482..36239
                     /locus_tag="PW224_000772"
     CDS             34482..36239
                     /locus_tag="PW224_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDL2907481.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            36256..37038
                     /locus_tag="PW224_000773"
     CDS             36256..37038
                     /locus_tag="PW224_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907482.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            37038..39224
                     /locus_tag="PW224_000774"
     CDS             37038..39224
                     /locus_tag="PW224_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDL2907483.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSA
                     YSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWG
                     LTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKD
                     SNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVY
                     TLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGM
                     LTGPSGTSSVSKAMYVRKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            39227..39673
                     /locus_tag="PW224_000775"
     CDS             39227..39673
                     /locus_tag="PW224_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907484.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            39675..39812
                     /locus_tag="PW224_000776"
     CDS             39675..39812
                     /locus_tag="PW224_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2907485.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            39849..40142
                     /locus_tag="PW224_000777"
     CDS             39849..40142
                     /locus_tag="PW224_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907486.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            40165..40362
                     /locus_tag="PW224_000778"
     CDS             40165..40362
                     /locus_tag="PW224_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2907487.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            40373..41392
                     /locus_tag="PW224_000779"
     CDS             40373..41392
                     /locus_tag="PW224_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2907488.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            41649..42296
                     /locus_tag="PW224_000780"
     CDS             41649..42296
                     /locus_tag="PW224_000780"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2907489.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            42579..42652
                     /locus_tag="PW224_000781"
     tRNA            42579..42652
                     /locus_tag="PW224_000781"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42613..42615,aa:Ile,seq:cat)
     gene            43198..>43766
                     /locus_tag="PW224_000782"
     CDS             43198..>43766
                     /locus_tag="PW224_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDL2907490.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT        15373 a         6996 c         9039 g        12358 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggcaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaatttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat agtagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatatcaac gattagacac gatttcgatc gtgtcttttt tgttgtatat
     3781 ttagcctaaa atgttttttg actccccttt tgactccccc tgagtactgt tcttcataaa
     3841 tgctacaaaa ttattgatga cttttttgtt ttgagtttca gttacatgag tgtaaatatt
     3901 tgaagtagtt tgaatgtctg catgccctaa acgttcctga acatctttta aagaagctcc
     3961 tgattcaaaa aggagagagg catgagtatg tcgaaaacca tgagtagtga ttcttttagt
     4021 caattctgga tgatgcttat atatgacatt aagccacgaa cgtgaaactg tagggttata
     4081 gagaagacca tcatggtgaa aaattaagtt ttccctttgg attgtaacga tcgttttgtt
     4141 ttttgtataa tactttctca ataaactgat caaatcattg tccagataaa tttttctttt
     4201 tccagatttt gatttaggag tatttacgat caatcttcct tgataacctc ttgttacagt
     4261 tttattgata ttgagtgttt tgctcttaaa gtcaatatcc gaccaggtca gagcaaaagc
     4321 ttctcctttt cttatacctg taaaagccaa tagggaaaag aataaatatc tttcatcatc
     4381 atcgtttctt attgcttcta aaaatttgat taattcttct ttagtataaa attcgatatt
     4441 tttatcttcg atatctagag cttctccacg tggaatagaa actaatttca taggattgct
     4501 agttataatt tgtaaagaag cagcgtaatc aaaaacatta gatgtgtaat tttttatttt
     4561 cttaaaaatt ttaggatggc tatcggacca agtgtttact gcttcttgac agaaaaaaac
     4621 gtctattttg tcaatgaact tatctccaaa cacttttaag atgtgtgtgt caaaaatttg
     4681 tttggtggag gaccaagtgc tttctttaac tgtcttttta taattttcaa accataagcc
     4741 gtaaacatat tcgaatttct tgctcttttg agctgtagaa gcttgcagtc ctttttcctg
     4801 tatatctgcc tccaatcttt ttaaagctct ctcagcggct aatggcgtac taaaacctcg
     4861 tctcgttgtt tttctttttt ttcctgttag aggatcaact cctaagtaca gactaaactg
     4921 atatttttct tcgccttttt ttgttaagtg ctttttaatt cgtttatcaa tttctttttt
     4981 tgccatcttt atctttcctt tcgtacgttt gttcggttgt acagcggatt tcgagatggt
     5041 aaaatagggt acaacaaata gacctacttt accgtagctc tttgcacatt tgcgttcttg
     5101 gtcgggcggc aaatgtgctt tttttatttc actcttagtt cttgtcctgg ataaagcatg
     5161 tagttgtttg gatccatacc gtttaatgca aacagttgat ctaccgtaat tccagctcgt
     5221 tcagctattt gtttggtccc ttcaccaggt tgcaaagtca aggtttcggt ttcagatgaa
     5281 gaaggtgccg ttggttgagc ttgattagct ggtacataat cttgtggtac tgattgctcc
     5341 gttgaactgg gagtgggtga aacttgttca acacttgaac taggagtagc ttctgcatag
     5401 ttttctacac tttcagcaga gtaagtacta ctagtttcag gaggcgtatt ctgcgcaata
     5461 gcagcttgtt ctacagtact agaagatgca atagtacttt ctgttgtagt cgaagatgat
     5521 tgttcgttgc tacttgttga gctagtagtt gttctagatt ctttagtttt tttccttttt
     5581 gtagggtttc aaaactaatt tctcattatc atcagatttg ttaggctttt ctggagttaa
     5641 aataatattt ttatcttctt tgctgacagt atatttggca tcattatctt tgtctttatc
     5701 atctttccaa gtcataacat cgccttcaag tgtatactca aatttgtaat tcatttggtc
     5761 gactaagctt ttagcaaatt cttctcccat agcctcccaa tcgttacttg aagttgattt
     5821 catagaactt gtgtcaacac taaaagatac gacgtgatca gaaaatgata cgatcatatt
     5881 aggttcgtca tctttggttg cttcaatcaa ccaatcatta gcttttaaat cttctgtagt
     5941 tactttttta ccacaagcag tgaaaagtaa cagagaagca aaaactaata aacttgttat
     6001 tacttttttc attttttatt cctcatttct atgatatgat ttttattggg gaatcttaga
     6061 aataaggttt cgagtccgtg ttgctgcacg ggcttttttt atttaaataa atcccaaaaa
     6121 ctgaatgtag ttttcttgta tactttattg taagcagcct tttttgggtc tttaatccag
     6181 ccgctacctt ttttcccata accaggaata actgcttttt ttacttttct ttttgctttt
     6241 ccagtggttc tagcgcttat ggattttttt atacttggtt ttctcattcc tattttcatc
     6301 tatatcctcc ttacgttgta ttataattca caagaaacga tcgaattcat ctggtaatcc
     6361 aaagcagacg agcttttcat attttgtcat atgatcaaag ccgtctaaag ttggatcaat
     6421 agtcagatga gttgcgaaat aatttgcttc tttttcaatt tttaattcag atactagcgt
     6481 ttttttagac aacataggtg tgttttcatt cggatgcaaa acacagtggc ctaattcatg
     6541 actacaagtt atccattttc ccatctcatc taaactagaa tttatgtgga tcatttttat
     6601 ctgtttgaat ttactatagt aaccataaat ttctcccaaa tcttcttcta aaactagtat
     6661 cttcaattct cgtgcaattc taaaaggatt gcgtgtttga tatagtttta ccagcttatt
     6721 aattttgtta tcaatttgag gtaaatacat aaacaacgta ctccttttaa tcgcgatatt
     6781 ttttaggtgt gtattttttc ttagcttcta ttttagctat tcgcaaagca ttctctaaag
     6841 aaataatgag agcttcacgt gtcgcttcac tcatttcatt atctttcttc gaaaaagcca
     6901 ttccatcagc attagataaa tcgtcgatta atgactgcaa ttctttttgg atatcacgct
     6961 cgtctttttc tgttaaatca tagtatcttt ttttctcagt acgtcctagc aaataatcta
     7021 cagatacgtc gaagtagtca gcaatttctt gtaacttttc ggcagttggc tgttttcctg
     7081 cgtttaaact ataaaagtaa ttctctccgt acttcaaatc tttagcgact tgttgaaggt
     7141 ttttattctt gtttttcgct agttctttta ttctttcaaa tgttgtcatt ccaacgttcc
     7201 tttccaacac gaaaaaataa tttacgaaaa agagtaaaaa tagctttaca attttacgat
     7261 atagagttat tatttatccg taagttaatt tgatagaaaa aagcaaacta aaaacacacc
     7321 ttatagcata aagtttggcg accaagtgca aataaaaggt tttgttatag gcttatttct
     7381 ctatgtattc attttactct atagtgtaaa aatgtcaatg gaaattatat tttttctatc
     7441 attttaactt acaaatttat aaaaaaagga agtgggttaa tgtcaaactt ggataacggt
     7501 cgtgcggcga tcaaagagtt tatgaaagag aatggaataa ctatggatga tttggctact
     7561 gcttatggtc acacgcgtgt acgaatgcaa caaattcttg atggacactg gagtggacca
     7621 aaggcaaaca gaactattct agagatcatc cgtgattaca gaattcgata ggaggcaagt
     7681 catgaatatt ctaagcgaag agtttttgac tcgtttaaga attgcaattg ttgaagttgt
     7741 aaaggacgca cttagtcaac tttcaaaaaa gaatttgtca gaaacacgat atttaaaaaa
     7801 gatcgaagct agaaaatatg tgggaggtgt aaacgatcaa ggttttgaga agttaatagc
     7861 tcacggttta aaagaaattc gtatagatgg ctttttaaga tatgacaaaa aggacatcga
     7921 tgaactgatg gctaaataca aaatttaaag gaggtcaaca tgggaaagtt taatcgaaca
     7981 ttagtattca gcgcgccact aattatctac gctttaggtc tatggggtag tagacaagca
     8041 ttaatcggca cgatcgttta catgctttgg attttcattg ggcttgatga agctgagtac
     8101 agagcgaaaa agccagtcgg gagggactga ctaaaaaagt ctaatgtcta taagaaaggt
     8161 ttgacaataa atgaaagaat tatttttttt aataactttc atcatttttt ctatcagtgt
     8221 tttctacatt aacctttgcg ttgctagatt tgctttgaaa agttttagga acagcaaacg
     8281 aaaatagagc tataaaagat acgaatgtag tagctacgtt aaattgctct ttagaaaacg
     8341 tcaatgtcat tggaatgata attgatatcg atgagaaaat ctttgcgata ataactatcc
     8401 atttttcaat tataaacatt tttaaattaa gcaccattag acaaaatgta gttcctatat
     8461 agaggataaa tgaagataga aataaacata ttagtccagt tttaaaaaaa gagaagtaat
     8521 ctatgtgggc aaattctgtt aatatgcttt tttgatttaa cacaatcaaa ctactgttgg
     8581 acaaaaatga cagtaaggaa agagttgctc cgattactaa ataaaaaata gttgttaaca
     8641 atattatatt atcaattttt ctgctcatgt tttcacccca ccagaattat atcaaaaagg
     8701 agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt gactacgagc
     8761 ttacaagttg cagaaagctt cgataaaaag cacagacatg ttttgacggc tattgatgaa
     8821 ctaaaagagg gggttgccga aaattgggca gacctatttt gggaagatac ttatgttcat
     8881 cctcaaaata aacaatcata ccgaattatt tatatgaata gagacggctt ctctctatta
     8941 gcaatgggct tcactggtaa gaaagcttta agttttaaac ttcaatatat tgaggccttc
     9001 aacaagatgg aaaaagaatt aaaagaccaa ttgccatcaa ttccagcaac taaacgagaa
     9061 atggcattac ttgctctagc tgcaaacgaa gaaacgaatg aaagagtaga cgctatcgag
     9121 tctgatttaa acgatttgaa gaacaatcaa cttctagctg aaccagatta ccgaacaatt
     9181 tctaatatgg ttcgcaacaa aattagagtt atttgtaatc agcagcattt aaatagcaaa
     9241 gcaaaagcag aattgtttaa agacttgaat ggcggaatta agcgaatcac tggagcagta
     9301 gctagaaatc gtatcaaggc aaaacagttc gatgatgtta tcgattttat taacaattgg
     9361 atgccttcta cagcgacgat gacaattatt aagcaaatgg agttgttaga ggatgaatag
     9421 agctgaagcg ctaaaaatag gcgtaataat tgctaatcgc tggtggagac acaataaacc
     9481 aaacatccta agccaacaac atattgataa gcaaaaagca tggcaacaaa taaaaaagtg
     9541 actcagccga ccaaagcaat gagtcacaaa gaaaatacat ctaaggagat gttaccacat
     9601 gaaaaaagaa ctttccactc tagatcaata tttgactgat cctagttggg gcaaatcgaa
     9661 tatcaaggaa acaagcaatc gaaaaatcag acgtaatctt ttgacggatg aagaactagc
     9721 atgtgatcaa gacgatttgg gaaattttgt gagtatttgg gatcatgtct atcttatcca
     9781 tttatcgagg aagtccaaaa aaactgaata tatctatgtc atcgaagatg gcttgattga
     9841 tgcgctagaa gagtatgaca gagataactt gattgatatc tcttattacg gatcaggtaa
     9901 gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aaaggaacaa cctttgaaaa
     9961 atagaagtga taatacgctt tttaatacct tatacaaaat aaatgtgaaa gatgttactg
    10021 aaaaacgaaa taacctcact tatctttcgt gggcatgggc ttgggcagaa gttagcaaag
    10081 tgtgtgaggc agtagattat gaaatctatc atgatccaga aacatatctg ccgtatgtct
    10141 ttgataagaa aacaggctat atggttttta ccagtatcac agtcaacgga gtaaagcgtg
    10201 acatgtggtt accagtcatg gatggtgcaa acaaggcaat gaaagatgag ccatatacct
    10261 acgaagtcaa tgattatcag tggaataacg aaacgaagaa aaaagagatt gttggaaaaa
    10321 tcgaaaagcg agttgaagca gcaactatgt ttgatatcaa caaaacgatc atgcgctgtc
    10381 ttgtaaaaaa tctagcaatg tttgggctag ggctatatat atttgctggc gaagatatgc
    10441 cagaagatgt ctcgatgctt gaaccagcta ctcaaagaag caaaaagcta ttcttggatg
    10501 ctttacaact ggttgctaac aagtacgaaa aatcaattga tgaagcaatt gttgcattga
    10561 ctgatgcggc ttctataacc gctgatgaca gtaaatggac caagagagac ttgggcattc
    10621 taaaacgagg cgttaactgg attgaagatc agtacagaga agaaacaaaa gagaagtgat
    10681 atgagtgttt aaaccattga tcgattcata ttcagcggtt ctgaaaaagt tcaaaggaaa
    10741 agacataggt gcaacgatca atgaagaagt gaacattgat cgactaaaga cgatgtatga
    10801 cggatatgat ggcgatcgga ttattgaagt tcgatttatt gatccacgtc gatttactgt
    10861 gcagcaacga aacttcatct atgcgctgat aggcgatatt ttcatcgata caggcatgcc
    10921 aacggacttc tggaaggaat tcttttactt ccgttttgaa ggcgtcacag ggcgcaaaat
    10981 aagcctcaaa gacgaatcga atacaactgt gagtgatgct aatgtcttag caaatatcat
    11041 cttagatttc atctttgaac atcatattcc tttcaaagaa ggctatgaga ttttaccagc
    11101 gaatcaagaa tattacttct acaaatgcat cacaaaaaga gtctgctgca tctgtggcaa
    11161 aacaggagct gacatcgatc actttgacaa agcgctagga agacgaaagc gcaaagaagt
    11221 tgatcattca gagtacacat ttgcagcact ctgcaggatt catcacacgg agaaacacaa
    11281 tataggtgtg atcaatttca aaaataaata tcaaatcaaa gggatcaagt taaaccagga
    11341 aacaattaag aaacttagaa taggagggta aaaaatattg tctgacaaca aacgctacta
    11401 ctatttaaaa ctaaaagaga attttttcga tagtgacgag atggttctct tagaaagtat
    11461 gccagatggc tatatttatt ctaatattct tctcaaactt tatttaagaa gtctaaaaca
    11521 cgaaggtaag ttgatgttta atgacaggat tccatttaac tctacaatgc tcgcaactat
    11581 tacaagacat tctgtaggag tcgtagaaaa agcggtacaa attttccgtg atttgcagct
    11641 tattgacgta ttagataacg gagcaattta tatgtctgat atacaaagtt tcattggaaa
    11701 atcctcaact gaagcagata gaaaaagaga atacagaaag aaaatagaag aggcaaaacg
    11761 gaatttaata actggaggac aagtgtcgga caaatgtcca gacaaaacta caccagagtt
    11821 agagatagag atagagaaag atatagatat agataaagaa gaaaagaaag gtaagtattc
    11881 tgacgaacac ttacgccttg ctaaaaaatt gcaaagtaat ttaactgaag attttccaaa
    11941 agaaatgaac aaagtagata tcgaaaaatg ggcagacaca atcaggttga tggaagaaag
    12001 agataaagcg tctatagaag cgattgagta tgtgatcaat tggctaccta caaatgaatt
    12061 ttggtttgga aatattagaa gtgctaagaa attgagagaa aaatttgaga agctcaaatt
    12121 cgaaatcaaa gcagacaaga ataatcataa aaagcaaagt caaaaactac agtacagcaa
    12181 tcctagtgaa tatgacgact tgccaattta aaaaggagat gcatcacatg gaaagcctag
    12241 caaatgctat ggagaaacta ataagaagag tattagtgca aagcgggaaa tgtccagaat
    12301 gtagcgaacc tttgtatagt tggcgagcta aaaataagga tggttcagaa cgttgtaaac
    12361 caacatgcat gagttgtggt tataaagcgt tacgtgtgaa agaggatata cagaccgaac
    12421 ggatatataa cgacagctta aaagcacgag cgttgagttt ttttcaaaat ggttcggtat
    12481 taacagataa aactttgttt aaatgcaaaa tggagaatta tcacgtagtg gaccaagaaa
    12541 cgaaaattgc tttagaaaga gctaaaagct ttgtaaatga tgtcttactg aaccatcctg
    12601 cacatttcat tctatcaggg aaatcaggaa gcggaaaaag ccacttgtca atggcgacag
    12661 cttgggaaat acttgagcgc tcaaattatg acaagaaaat actttttata agctatcaag
    12721 agttattaga gcaaataaag ttttcttata acaatgctga actgagaaaa gaaattgaag
    12781 gatcgcttat agccgatatc aaaacaactg atttggtggt ttttgacgat attggagctg
    12841 aattaggtag cggggtatca aatagtaggc agtttacaaa caacacgtta aacacgctct
    12901 tagaagccag acagaacaag gcaacgatca tcacaacaaa cttatccggt cctgaactaa
    12961 gagaagccta cggcgaaaga attgtttcta gaatatttaa gaattcagaa ggttatgcgc
    13021 tgaaattcca acaaacagca gacaagcgca taaaaccagt gaaaggtagt atcgcatgaa
    13081 taaataccgt aataagaaaa ctgttcatcg aggtatcaag tttgattcta tcgcggaagc
    13141 agagtattac gatttagcct tgtggcaagc agaagcgaac ggctggaaag taaaacttca
    13201 ggaaagattt gagctgatgc cgaaatttga actagatgga aaaaagtatc gcaagatcga
    13261 gtatattccc gacttcacat tttataaaaa cggcaaactt gtcaaagtcg tagatgtcaa
    13321 aggaatgcag acaaaagact ttaagatcaa ggcaaagttg ttctgtcatc aatatcaagt
    13381 gccgttgata ttagctaaaa aatatcggaa tacgttcaag gaagagcgtt tttaacgagg
    13441 tggtccatca tgacaacaga agaagtgatt caaatgcgta ttcgaagcct tcaacgtgag
    13501 attgacgatc tggaacggat aaaggcagtg atggtcaatg aaacggcgag aaaggcaatc
    13561 aatttgcaca tagtgaattt aagaagggaa attcgtagat tggaggaatg agcgtgggta
    13621 agaaagcagc aatgaaacga atcattgaac tgacacattc tgagaattgg caagaagaca
    13681 aagaaatagt tgcagaagtc caaaagctcg gtaaatcaat gtggactgaa aagcctaaac
    13741 ggaaaacgcc gagaaaaatt gcaatctggc atggtgatcg aattctagta acgggtacag
    13801 ctgaacagtt atctgaaatt actggactga gcaaaaacat catctgggat agagctagga
    13861 gcttatggat tgattcaaag gggcgacagt ttaggtatgt ggaggagaaa taatggatct
    13921 cattacacaa tacagtgata tcatcctcaa gaaaatcatg atgaagattc agaaagataa
    13981 aaaagcaaaa gaacgagcgg aattagttaa gttggaaatg gctgaaacag gagcaggagt
    14041 gcgaagtagc aggcattgga aagcagcagc aaacattgaa ttttattaca acgaaattca
    14101 aaaagggttc gatcagatgc gtgagctgga tcggcaaaca aattggagca agaaacttca
    14161 tcaggatcgt ttcaaatttg tagaaaagta tagagagata ctgaatgagt atttcgagga
    14221 ggactagatg tttaattcaa cgttagcgtc aattcacttt ttattctata taacgatatt
    14281 tgtagctata ttaattaatg cgataaaaag cggtttttta acaccaatcg gcacaatttt
    14341 aacattagct tcatttgtta caagttgtat tatccaactt aaatatataa aggagaatga
    14401 ataatggacg aactaatcac aaaagtagag cagtgggcta aagataaggg attggatcaa
    14461 gctgattcca gcaagcaaat gttaaaaacg atcgaagaga tcggggaagt tgccgcttct
    14521 ctagctagaa aagatgaaca tggtttaaga gacggaattg gagatgtagt agtaaccttg
    14581 attattttag ctatgcaaaa tgatatggat ttgtacgagt gtctgaacca agcatacaac
    14641 gaaatcaaag gacgcacagg aaaaatggta gatggtgtat tcgtgaagtc gagtgatttg
    14701 gaggacagca aatgatcagc ctaacaatat tttggtgtgt aattggcggc ttactagcga
    14761 tgttaggagc atacctcatt tacgagagtg caaaaggcaa aaaatataca gcagtaggga
    14821 ttattttgtt tgttgtcgga attttagttg cgattggtgc agaaataaat ctttatttaa
    14881 acggtacgac agaagagttg atgtggtttt tattgtggac aaggagatag gaggacagcg
    14941 aaatgggcga tatgggcgaa tactggaaag atattaaacc gcatctaaaa gaacgtagaa
    15001 aacaacatgt cagaaagatg ggcaattcag caactaaaaa tattgaaacc ttgggatttg
    15061 aatttactca ttatccaaac aatcatcaat ttgcaattaa tacccataat ggaattattg
    15121 actactgggg aactacagga acgtggattg agcgcaaaac aaaaaaacgt ggtaaaggga
    15181 tacgtagttt gaggaaatat attaatcagg aggacagcaa atgaactata cacaacaaga
    15241 actgactgat ttatgtccaa aacatgtagc tgagttcatc aataatgaag tcctgcctaa
    15301 atatgcagat ggtttaaata cagcagaaaa cgttacagat tttatgatta atgatgctat
    15361 tgatcgtttg atatttttag aaatagattg tattgcttat tatcgtctac acgcagaagt
    15421 tgctttgatt gatccgtata ttgctttaag ccaaaatcga aaaatactcg tagcctatat
    15481 tgataccaaa cagattttag aaggcagaga tgtcgagata gagatcgatg aggaggaagt
    15541 gggatgaaag catacacaat caagttttat ggaaataaaa cctatgtata catcctaagc
    15601 ggtctcagac ttgattttag aggctattca aaagaagacc ttttagaatc cttatatggt
    15661 tacggatatt taaatgaaga tgaactaata agcttagaga aatttacaaa tgcttggacg
    15721 attgaggagg aagcagaatg aagatttatg tagtaaagtt cgggaataaa ttttatagaa
    15781 gtgatgaacg ctctataggg gctaacacat tattcattgt agacatactc caaagtgcga
    15841 gatggtttga tagcctcgaa gaagctaacc aagtttcacg acgacttggt ggattaacac
    15901 aagtatatga actggtcact gtcgatcatg aggaggtaga aagatgaaat taaaagacgg
    15961 attttacgct agtagtcatg gtatcggcgg tttaatgcta gatatgccga caaagaatcc
    16021 taaaaaacgt aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg aagcagaagg
    16081 gttcatctat ccatttcgtg gatatgtaga acacgtctat aatcactcag cgatcattcg
    16141 tattgaaaac acgatggaat gtgataagtg gttagcgaaa agcaaagaga atttagctgt
    16201 agcgagattg gtggatatga aggctatata aacagcgaaa aaatgtactt ctaagagaaa
    16261 aatttatttt ttataaaaat aggaaagtat cattatgaaa aagaccacag agataaataa
    16321 acctaccaca taaaaaatga atatttcttg cgattcaata taggtatatt taacaatttg
    16381 tacaataaaa aatactatag gtaaaaaaga taacatgagg agcttatttg ttctagagtg
    16441 tttgacaaag taacaaaatg cgacgatacc aatacaaacg ggaatccaaa ataacagcat
    16501 gctccacata gtcactcaac catctcctat acgctttatt atcaatagat aatatcattt
    16561 ttcgtaataa agataaaaaa ataagtaagg cttctcatta aaatataaaa aagacagccg
    16621 accactggct gtccttagca gaatattgaa atagtgagcc atctgttttc cgccagatag
    16681 ctcaacatgt atatgggtaa cgagtgcaac ctcgctactt gaaaagctta gcataagcat
    16741 gggtataaaa aaagccggat tcctccgacc ttaattaata attctgacac aattattata
    16801 tcataaatgg aggaatcaat ggatggtact tttcgatgta aagaagtatg agacaccaga
    16861 ggcaaaagat gtggatatgg atcgcacaaa acataatgtt ggtgtttttc tttcagcata
    16921 tctgtcagct agatgtagga taggtcaacc tcgggagcct aaagtaacgg catcttactc
    16981 cttggttcca ccttctacag caaataatac atttgaagca gaacgaatga tgattgagag
    17041 agaggaagcg caagaagaat ttgagtactt gcacaaatta tttgttcgtg gctattcagc
    17101 aattcagcat ccgcataaac ctgatgtaac agaaaggcgc aagaagatat tctatgatcg
    17161 ttatatcaat ggtctgccca tttatgtaac tgctcaaagg aataatacta gcgaagaatc
    17221 ggttaaagta gaatcaaaca gaattatcat ccaatttgct tcatcgttag aactggttgc
    17281 ttttaagtag ccagttttta cactttttat accctttgat tacccatttg gtttccattt
    17341 tatacctttt ttataccaat cacttacctg gtaaatgatt tattatgata gtgtcgaaag
    17401 attagtgata ggtctgagac aaaataataa taaaaggaac atcgttttat tattgtttca
    17461 caattaagct tcgatagaca gcaacggaaa tattaagaat aaggatgtga attccaactc
    17521 cttctaaatt gttcttatta tctatcatcc gttgctgttt attaatttat gtattggagg
    17581 gaaaacgaat ggaagtcgag caaataaagt atcaaggtat ggatcgggaa cggttgattg
    17641 atattattga gcaacaacgt ttagaattga tccaaaaaga agctgtatgt aaagagtaca
    17701 aaaaacattt agaacaagta attgagtatc attcagtaga aaagtacaga tcagtggtgc
    17761 aaaaaaatag agcaacgagt cctgatcctc gtcaagatgt atcgattagt atcaagacgc
    17821 cagtatttaa ttctgttaaa tcagaaaagc tagaaagtat ctcaactgct ttggtagaaa
    17881 aagcagctga aatgaactcc ctagcaaatg aagttagtcg tcttatttag tcacatcgat
    17941 aacttgagag tctttggtga ttccgttttt tatagaatta atggtttgtt cagcggaata
    18001 tttagtcaag tatgtttcgc tagttgctac tacttcattg ttatcggact taataacgaa
    18061 gtaatattgg ccattagttg cttctcttat aacaaagtac aaatttttca ccacctttta
    18121 atttatttca gcggaccact cgctgataaa taaaattata cacttagtat ttattttcac
    18181 aatattaatt tgtcactgtg gcggaaaggg tagacgctaa gcatgtgtgt taggtcaatg
    18241 cttcggcaac catgcaaggt tcgattcctt gccagtgact ttggcaactg agggttggaa
    18301 atgggcgctc aaagtacacg agcaaggcga ggtcgatagt aatcgatgga atcggtgtag
    18361 gttgcttgat agaactataa ctgcatctcg tttatgaggt gtagttttta catattagat
    18421 cactcatcga gtggtctttt tattttggaa ggggagtaaa aaaatgaacg aaaaccaatt
    18481 aagagagttg tttaaaacga atgaagcaaa caaaacaatg gaggcgacat tctacgaaac
    18541 tcaaaaaagc ttagcgttaa tcgcaaaaca agctaagtat ttctacgatc agcttattct
    18601 gcaaggattt aatgacgcac aggctatgga atttatgatg cgaacctttt ctgccagtaa
    18661 ccaacagaaa gagtgatacg taatgaggaa ctactggtat gtatcattat caaataaata
    18721 tccgcaacca aacgcagatg atccaattag agttgtccag tcagtccaga ttaaaaagaa
    18781 gtactccatc attgaaatga ccagagaagc tacgccaaaa gagattgata agtacaatct
    18841 tcgttactgc ggccatggat attttagtga gcagaacata cagacaaata ttgagagata
    18901 ttgttaataa aattgaaagg tggtggcttg aatgtggtaa atttgacacc aaaacaaaaa
    18961 gcttttgcgg atgaatatat aaaaaatggt ggtaatgcta ctcaagccgc cattaaagca
    19021 ggttatagca aacgatcagc aagagtaata ggtaaggaaa acctaactaa acctaacata
    19081 atacagtata ttaatgaacg gctaaatcct atcgaaaaga agcgcaaatt aagtgccgag
    19141 gacgctttaa atgaattgat agatatttgg caaggagaag tacaaataag cgtgagcaag
    19201 caaatagacc gcttggagaa aaacaaggtt attaaacata tgcaatatga atatacacca
    19261 gatttagaaa gcaaattgaa agccttggat ttgtatttga agtataaatc gctgttatca
    19321 caaacgcaat tagaaaaagc tcaaacagaa ataaaattaa tgcaagcaaa attagaacaa
    19381 ttacagataa actcagagcg ttctaccgaa gaaaaacttg atgagttgtt agaaaagatt
    19441 agtggtgaat tagatggcac tagttgatat ttataaccca aagcaaatcg acgtgttaaa
    19501 taaaaccatt aagaatgatt ggtttattac tttattacat ggagcaaaac gttctggaaa
    19561 aacaaaaata aacaatgatt tgttcttgtt tgaattgcga cgtgtgcgaa agattgccga
    19621 tgaagaagac attaaggaac caatgtatat cctagcagga gtttcaagtg caacaatcca
    19681 aaagaacatc ttacaggaac tatacaacat gtacagcata gaacctaaat tcgataaaca
    19741 tggaaacttt aagctatttg gcgttaaggt cgtacaagct tatacaggaa acattggcgg
    19801 tgttggcgca attcgtggta tgacagcata tggcgcttat atcaatgagg catcgctagc
    19861 tagacaagaa gtatttgctg aaatcgtttc acgttgttca ggaacaggag cgagaatact
    19921 agctgataca aaccctgaca acccggaaca ctggctaaag aaagagtata tcgacaattc
    19981 aagcaaaaat attcaatcgt tccactttga attagatgat aatacatttt tatctgaacg
    20041 atatcgtaac aatattaaag aatcaacacc aagcggcatg ttttatgatc gtgatatcaa
    20101 aggtttatgg gtttctgcag aaggtgtcgt ttatcgtgat tttgatgcta gcaagcacta
    20161 catccagtca aaagacttac cacctttgag caacttttat tgtggtgttg actggggata
    20221 tgaacactgg ggttcaatcg tagttatagg tgaaacggat gacggaacag cttatttaat
    20281 cgaagaacat gctactcaat ttgaagagat tgattattgg gtagatgtgg caaaaggcat
    20341 tcaagagcgt tacggcttac gagtaccttt ttattgtgac tctgcgagac cggagcatgt
    20401 ggctaggttt gtaagagaaa agattaatgc taaaaatgct cataaagcac ggttatctgg
    20461 agttgaggaa gtcgccaaga gatttaaaca agataaattg tttatctgtc aagatagagt
    20521 gatgaaattt cgggatgaaa tttatcaata catttgggac aagaaaaaag gcgagccaat
    20581 aaaagaatat gatgatgtgc tagactccgt tcgatatgcg atatatactc atgagcttct
    20641 taagaaacca aaagttaatg tcaacgaaaa gattaaacgt attaaacgca tgttttaagg
    20701 agtgtgaaaa atggataagg taaacgagtt tgaatacgga gctgatatac attattctaa
    20761 tgacgtgaac acaaattatg taaagtttag cgtagactca aatcttcact atagatttag
    20821 ctcagcagaa gatttactta acgatttaga tactttagca gcaatgataa aacatcatca
    20881 tgaatatcag gtaaaaaggc taagtgtatt agatgattat tataaagcta gaaatacaaa
    20941 tatcatggat aaccgtaggc gcagagaaaa agaaaaagcg gatcaccgat cagcacataa
    21001 ctttggaaaa gttctttgta cgtttgatgt tgggtacaac acaggcaatc ctataaaagt
    21061 gcaaatcgag gacacaaatc aacaaaaaga aatcgaagag tttaatacta ataatgacat
    21121 agatgggtta aatgctgaac tctggcttga tatggataag tatgggagag cctatgagat
    21181 tatctatcga gattcagatg atacagatta tgttgatttg gctaatgtat ttgaaacgtt
    21241 tgttgtatat gatactacag taaaacgaga gcctattttg gctgtacggt atcctaagac
    21301 aagattcaac aaggatgctg ataaacagta cattcaacca atcgtataca caaaagaaaa
    21361 aagtatcact tatgatgaga cgacactaac agcaattgag ttaaagaatc cccaggatga
    21421 accgcatgaa tataaagagg tacctattac agagtattct cctaatcgtt ttcggatggg
    21481 cttgtatgaa gatgtactat ctttgattga tctatacgat gcagggcagt ctgataccgc
    21541 caattatatg actgatctaa acgatgctct tctagttatt agtggtgata ttgaagcagc
    21601 aggactatcc acagaggacg ccatcaagca gaaagaagcg aatatgcttt tgcttgagtc
    21661 tggaactgat gtgaacggta ataaaacaag tgtgactgca ggatatattt acaaacaata
    21721 tgatgtgaac ggtgtagaag catacaaata cagagtacgc aaggatattc acgaaatatc
    21781 catggttcct gatcttactg atgacaattt ttccggagtg caatcgggag aagcaatgaa
    21841 atataaatta tttggatttg aacaaatgac ggcaacaaag caaaggctat tcaaaaaagg
    21901 ccttatgcgg cgttatcgtc ttttatttag cctaaaatca agtatttctg aaatggataa
    21961 ctccgatttg aaaggcttac gtgtaatatt tacgcctaat ctacctaaag ccattctgga
    22021 agagttgaaa tctttggttg atgctggagc tgaactcagt caagagacga tcttaggact
    22081 cgcttctttt gttccagatg tacaggcaga gttgaaacga gtaaataaag aaacgcaaaa
    22141 gcagattggc atttttgatt cagatggtga agaagtaatt aacaacaaaa aagatgaaac
    22201 aggggggtga ttaaatgaac tcccaagaat attggatcaa acgggaaaag gaatggcaaa
    22261 agcaacaaat taaagatgat aaaaagcgca tggcagaaat taaaagtcgc atgcaatacg
    22321 cacaagatgc gatacaaaaa gaaatagacg cgcagtggga cagtttctcc aatggccaga
    22381 aaatcactcg tagcgaagcg atgaagcgtg ctagtgaaat ggatgtcaaa gcattcgctc
    22441 gcaaagcaaa gaagtatgtc aaagagaaag atttttctcc tacagcaaac caagaattaa
    22501 agctatacaa tcttacgatg cgtgtaaata gattagagct cttaaaagct aatatcgggc
    22561 ttgaattgat ttcactgttt aatgaattgg ataagtactt ttcgaatgaa ttaacaaaag
    22621 ctggtttagc tgaattgaag agacaagccg gtattttaga aatgactatt gcttcaagtg
    22681 gatatgcaaa gctgatagaa ctagtaataa acagctcctt tttgagtgat gacgtgtctt
    22741 ttagtgatcg cttatggatg tatcaatctg aattgaaatc agaattagat aggttgttag
    22801 tcagaagtat aacgatgggg aaaaatccca agcaacttgc atctaaattg gcagaatatt
    22861 taacagctga aggacgagaa aacactaagt tcaacactca acgtttgatg gtgactgaaa
    22921 cgactagagt tcaggtaggg attcaagaac gaagttatag agatgcaggc attacccagt
    22981 acatctatat agcagaacca acggcgtgca aactatgtat accgttaaat aatcaagttt
    23041 ttgatgttgc cgatatgcag ccaggaagta acgctcctaa tatgcatcca ttttgtcgat
    23101 gcagtacagc accttatata gaacgaatat caagccgtta acacaaatta acggcttttt
    23161 attgtgcctt cttacagctt acaggcgtta aagagaaagc tattttcggc tgaccggcgt
    23221 aactggtcaa atttatcggg tagcggcgta accgtggagg attaatcatg aaaaaacgtt
    23281 tatttatgcc aatgaactta caattttttt ctgaaccagg agatggtgga tctggtgatg
    23341 agggacaaca aggaaaccta ccagctggct cacaagagac accgcccgaa gcaaaagaag
    23401 aaaacaatac tggcaaaaca ttttctcgtg atgaagtagc gaaaatgatc gctgctgaga
    23461 cgaataaagc aaaagcagcg tgggaaaaag aactagcagc aaaaaaagaa gaagctaaaa
    23521 agctggcaaa aatgaatgcg gaagaaaaac tacagcatga gttggagcaa aaagaagctg
    23581 aaatcgctga attaaagcgt ggacaggcac tatctgaaat gacgaaagaa gcttctaaaa
    23641 tgctgacaga tgcaaattta ccacacgatg atgatttact tggtctgatt gtttctgatg
    23701 atgcagatgc cacaaaacaa gctgtagcag tcatcactaa ctttgcttct ttgattaaga
    23761 gagaaaacgc aagacaaaca ccaccaaatg aaggtggaca atttacagca tcgaaaaata
    23821 ctaaagaaac agtggctaaa ctagctgcta aaaatcgaat tatcaaatag gaggaaaact
    23881 taatgaaaaa gaaacaactt ttaccaatga acttgcaaat gtttgctcaa acatgggatc
    23941 cagataatgt cttggtatat gaaacgaaag agggaaaaat tcctgataaa tataatacgc
    24001 tcattttgag tgaagttatg gaaaattcta agatcatgca gttagcaaaa tacgaagaaa
    24061 tgactgacaa agaaaagaaa tttgaatact ttgcagaagg accaggcgca tactgggtgg
    24121 gtgaaggtga aaaaattaaa acgtctaaac ctaaatggat gcaagctacg atgactgcaa
    24181 aaaaactcgg tgtcattctt ccggtttctc gtgaatattt aaattataaa ttatcagatt
    24241 tctttgagga gatgcagcca aaaattgctg aagctttcta taaaaaattt gatgcagctg
    24301 ccttattaaa taaagaaaac ccatttcctc agtcactaga cggatcagtt attagtgcgg
    24361 ggaatgtggt tgaaggcgga ttgacttatg ataatatcct agccttagaa gacaagttag
    24421 cagaaaatga attcgaacct aatgcgttta tttcaaaccg aaaaaatcgt acagaattac
    24481 gttctgcagc tcaaacagtc gggtcaaatg ttgagtttat ttatgatcgc tctactaata
    24541 caattgacgg attaccagta gtagacctta agtctttaga taaaggaaca ctttacgctg
    24601 gagactttaa ttacatgttt tatgggatcc catataatat ttcatttaag atttctgaag
    24661 aagcccaatt gtctacttta actaatgaag atggaacccc agttaacttg tttgagcaag
    24721 aactgattgc tttgcgtgca acaatggatg ttggatttat gattgtaaaa gatgaagcat
    24781 ttgggaagat ttccccaaaa gcgtaacgcc tgctaccggt attgtgccaa atcaaaagac
    24841 atggaccggt aaagtaggcg atactaaaac atttactatt tcagctgtgc ctgcagatgc
    24901 tagcgatgca gctgctgttg ttgcagctac tacagcaact tcaagtgatg gagctatcgc
    24961 aacagtgacc aaaaatgaaa atggtggttt tgatggaacg attgcagcag aagggtcagc
    25021 aacattcaca tttacttctg gagaattcac tacttcaatc aatgtgacag gtcaacctgc
    25081 tagttaggaa gtaaaaatat gatgattgca gatgatatta aaaaacttct taaaggaaca
    25141 atagatgaaa agcttgaagt tattgagcga agaacgaatg agcgtatgaa aaccttgtta
    25201 aatacgcaag aagttcctaa agaatttgaa acagttgtat atgaagtatc gttgaaaaga
    25261 ttcaatagaa ttggtcaaga aggtatgcag tcatattctc aagaaggttt atctatggct
    25321 tttcctgact cggatttttc agagtatcaa aatgagattg atgaatttaa gcgtaaagat
    25381 caggaagagt tgtacaagcc aaagcgaggg aggtttaaat ttgtatgaga tttacagatg
    25441 aaattatatt tgttaaacgt tcatctgact ctaaatatga tccagatctc ggtgagtggg
    25501 ttgaaggcaa accagaaaga acaagaacag aggcaaacgt gacagatatt ggcactgata
    25561 gaagtgtgac tatttttggt agtgtggaag aaggggcgaa ggtcattagg acgcagcctc
    25621 ttttttctat ccctacattt gactatatcg agattgaagg aaagacttgg caacaaaaaa
    25681 cagctagaaa tccagcatat agaaatagtt taattgtgca agaggtggtt cttgatgaag
    25741 gcacaacttg aatataaagg aatcgatcag ctgatgcgac atctgaaaaa agcagcaacg
    25801 cttaatgacg ttcaaaaagt cgtgaaaagt aatactgctg aaatgactga acgaatgcaa
    25861 aaaggtgcgc cagtggatac aggttactta cgaagatcaa taaacatgaa tcttttagaa
    25921 gctggtttaa ctggtattgt aggaccgaca gcagattatg caccttatgt agaatatggc
    25981 actcgattta tgtcggcaca gccttatgtt agaccagcgt ttaattacca aaaagtcaaa
    26041 tttatggctg aaatgaaagc cttggtgaaa tgatgattaa gacaagagat caatcaattt
    26101 ttgatgaact ttttaaaata tcccaaaaca aacttggata taaaacatac gattacaaaa
    26161 ctttagagga tgttggttat ccttttgtgg aatttgagaa cactcagaca atccatgaag
    26221 taaataaaac tgacattaaa gggtctgtga ttgtggtttt atccgtttgg ggattacaga
    26281 agaaacgaaa gcagttgtca gatatggcat ctgctctttt taatgaagct agattgatag
    26341 aagccacaga aggctattat tgggctttaa attatcaagc aagtggaatt caagtgatgg
    26401 acgacacaac aacgaataca ccgctaaaac gggcggttgt cacacttgaa tttagaatta
    26461 gataggagga agaacatgga agcattaaaa ggtattgatg tcattttgct ttatcgctta
    26521 ttgaaaaaag aaactcagga agccgcttgg aaaatggcat ttcaaacaga acacgaaaat
    26581 ggtttatcaa gagattcaga ctctacagtg acaaaagatg gaaacgttca aagtttaagc
    26641 ccggttgaat atgatttttc ggctacttca atagttgcta aaggagattc tcatgtagat
    26701 gaaatgaaac aagctttatt aaatggggat atcattgaaa tttgggaaat taataaagca
    26761 gaacagggaa cagatgataa tgcaaataag tacaaagcta cttattacca agcatatgtg
    26821 tctgaattta ctccatcggc tgctgcagag gataacgttg aattaagttt atcatttgca
    26881 gtaaatggtg ttggtcaaga tggttatgca accttgacag aagatcaagc cgatgttgtt
    26941 caatatgcat tcaaagatac cgtgaaagca acttcgacag gagcataaga gggcttagat
    27001 gctctctttt ttattttagg aggatgaaaa acattgaaat taaaaattaa aggtaaagaa
    27061 tattcgttta aatttggcac taaatttgta cgtgaattag ataaagtgat gcctttcgtc
    27121 gatggaaata tggaatttgg aatgggactc tccgcaaaag tcttaccaga gctacgttct
    27181 tataacgtta acacgttatc acgagttctg gaaatagcaa atagaactga agatgaatct
    27241 attacattag atgaattgga tgattacatc gatgaagtta aagacattga aaaattgttt
    27301 gatgaagtcc taaaagaatt ggcggagtcg aacgcgggaa agttagcggt ccgaaacctg
    27361 aatcagaaat tgaaagaagc ggaaaaacaa caagtggaat agattctgca ctggcatacg
    27421 aacaaattct tatcaattct tttcgatatt tgggaatgac caatatctca gatatcgaaa
    27481 gaatgacgtt atatgaatac aacattcgta tgactgcagc ccagttatct tggcttgaca
    27541 aagaaaagtt gattcacgaa ttagcgtggg caaatcagca agtccaagcg gagaaaaaag
    27601 taggcaaaaa gacagttcct gtatatcgat cctttgaaga attcttcaat tatcaaaaaa
    27661 tcgaagattc aatcatggga gtttccgaac tttcaaaaca agataaaaaa ttccaaagct
    27721 tactaactaa agctaactct tgaggaaagg aggagaatca tggaacaatt ttctgttgaa
    27781 gccttattaa aagccacaga tagtggattt gtaaagactt ttaaagatgc acaagatgct
    27841 gttaaaactt ttgaaaagaa ttcaaatagt atgacaaccg ctgttggtaa agtgatgcaa
    27901 ggtactggtg ccgcaatgac aaagtatatt accactcctc ttataggagt aggcgtagca
    27961 gctgctaaag ttggtggcga cttcgaagca caaatgagtc gtgtaaaagc tatatcagga
    28021 gcaaccggcg acacattcga acagatgaaa cagcaagcga ttgatctagg agcaaaaact
    28081 gcttttagcg caaaagaatc agctgctgga atggaaaact tagcttctgc tggatttagc
    28141 gcacaagaaa tcatgaaagc aatgccgggt cttttagact tagcagctgt atctggaggg
    28201 gatgtggctc tagcttctga aaatactgct actgctttga gaggatttgg tttagaagca
    28261 agtgaagcag gacatgtcgc tgatgtattt gctcgtgctg ctgcggatac caatgctgaa
    28321 gttggagaca tgggagaagc attgaagtat gttgctcctg tagctaattc aatggggatt
    28381 tctttggaag aaactgcagc agctattggt attatgagtg acgcaggcat taagggttct
    28441 caagcaggta caacgttgcg aggagcattg tctaggttag caaggccaac aaaggctatg
    28501 caagatacaa tggataattt aggtgtttcg ttttatgatg ctgacggtaa aatgaaacct
    28561 ttaaaaactc aagtagaatt acttaaaaaa gcttttgaag gcctgacgcc tgaacaacaa
    28621 caaaatgctt tagtaacact atatgggcaa gaatcattat cagggatgat ggctttgatt
    28681 gataaaggac ctgattcatt gggcaaatta acaaaatctc tgaaagattc tgatggtgca
    28741 gctgacgata tggctcggac catgcaagat aatatgaatt cttccatcga gcaaatgttt
    28801 ggagcttttg agtcagcagc tattgtaatt caaaagattc tagcaccatc catcaaaaaa
    28861 gtagcagatg ccatatccgg cttagtagaa aaatttgtaa gtgctccaga atcaactcaa
    28921 aaattagtag tggccatagg agcaattgtc gctgctatag gaccgttaat ttttatgatt
    28981 ggttcagtaa ttatatggat caatagggtg aaagtagctt ttaaagcttt aagtgaaagt
    29041 tcaaaattgt ttagtggatt aagtaaagca atgggtcttc ttacaaatcc ggtttttctg
    29101 gttatagctg cggtagcact actcgttgta ggtttcatct atctttggaa tacgagtgaa
    29161 gattttagaa acttttggat tggcttatgg gagggaatca agtctgctgt aagctcggca
    29221 gtagaatgga ttcagaatgc atggaaatct acaggagaat ggtttaacaa tttatggaag
    29281 tccattaaag aaggcgcaga caatgtttgg actacagttc aagaagcccc tgggaaagcg
    29341 gcagattgga ttaaaaacaa atggactgga acaaaagagt tcttttcaaa tttatggtca
    29401 agtattgcaa actctgcttc agagatttgg aatagtttaa aagaaggtgt catatcagtt
    29461 attgatgatt tagtttcaag tgctggtgaa aaatgggaag ggtttaaaaa tactatatct
    29521 actgcatgga aaacaattac aagtaaaatc aaatctggtt ttgattttat actaaaatat
    29581 attggtccat ttgtaagtag cttttcagat gtgttctcta atatagtgaa agcaataaca
    29641 agtatatttg ctgaggttaa aaacataata gtaaatgctt gggaaatcat taagtcttta
    29701 atagctgcgc cactactgtt tattatagat ttaattactg gtgacttcga acaaatgaaa
    29761 gaggatttag atctaatctg gaacacactt gtccaatcag tggtaaatat ttggacatct
    29821 gtaaaaaata tatttacgga atatatcggt gcaatagtaa atagtgccgt tagtttatgg
    29881 actggattca tacaaagtat ttctaatatt tggaatgaag tagtttatca agcgactatg
    29941 atttggattg atttgaaact attttttact aatttatgga ttgatattaa atacagtgca
    30001 attcaaatgt ggataaatct aaaattctcc ataattcaaa cttggattga tacaaaatat
    30061 ggtgcaattg aactttggaa taatctaaaa caatggtttt tccaaacggt taataatatc
    30121 gtgcaaactc ttataaaaag ttggaacagc ttaaagcaag gaacgataga tttattcaac
    30181 aatacagttc aaggtgctaa agacatttgg acttcattca aatcttggat tggtgattta
    30241 attactggaa ccaaagataa catcattcag ggatgggaaa acctaaaaca aggtactata
    30301 gatactttca acaatttagt aaatggtgct caagaggcat gggataattt agtaaatgct
    30361 gttagtgaca cggttgatag agtaactggc tggtttgata acttgaaaaa tatcgattta
    30421 ctagcagccg gaaaagctat catggatagt tttctagaag ggttacaaaa tgcatggaaa
    30481 tctgtgcaag attttgttgg aggtattggt gattggattc gtgaacacaa aggacctatc
    30541 caatacgata gaaagctatt gattccagct ggtcaggcta ttatgaacgg tctgaataaa
    30601 ggtctgacag gaggattcaa tgacgtacag aatactgttg gaagtatggc agactttatc
    30661 gcggaacttt tcaatgcaaa ttctgatgta gatatagctg caaatttgaa aaatgcaaat
    30721 aaaaacattg gtgcacaagt tgaacataaa ataaatatgg gtggctctac taaaccagct
    30781 gtatttaaaa tcaatcttgg aagacaatcg tttagattgt ttatggacga tatttcacaa
    30841 gctatgggcg aaggtgcaga tattaatctg gaattttagg agggaatatt ttggatcaac
    30901 gagaaaataa aatgtactca ttcaaagata caaccattaa tctcactagt tctaaacgat
    30961 tccttccgac gtctgccatg atgtacgatg gaatgtattt agaagatttg attgatggtt
    31021 atcaaacact tacggtggaa ggtagagaaa tgctttctgt agaagttgaa cagcaagaga
    31081 tacaaattgg ttcaatcatt acaaatcaga aaataccttc aagaacacta aaaataacat
    31141 ataagctgga agatagagat ccagaaaaac tacagtttaa attcaaagaa ctgttgaatt
    31201 atttataccg gaatgaagac gtggaaatta ggtttcatga tgaattagat tattattact
    31261 acggtcgcta tacatcaact gatactgttc caggagactc caactcgatt atttcgagtt
    31321 ttaatgtatt ctgtgcggat ccactaaagt atacgaaaga gtgtgttagt gatggctata
    31381 ttggaaatcc gatacagttt cctataacac caagaaaaat tgaagttact ttatccatga
    31441 ataattcaat caaaattaca aacggagaac aaaatatcac gataactgac gcggcaataa
    31501 aaacaggaga cgtgttggtt tttgattttt ccgatgagca ggtaactgta aacggagaag
    31561 attgtacttc tatgattgat ttagaaagtg attttgagaa cttttatctt aagcaaggtc
    31621 agaagataac tagcaataat gggaagctta aaatattcta taggggggcg acaatttgag
    31681 tgagacagtt tatttctttg atcacttgca aaaacttatt aaaagaaaaa atacaagaag
    31741 tttgattgaa gtctcccaag aaaaagaaat tagttctgat aagagtgatc taatgaaaga
    31801 tactctttac gttacgacaa aatatgataa agaaatagag gatgcaagat atatggcgat
    31861 tcgtgaaaac gagtcgtctt tttcgttgta tcgaattact aaagttagcg acccatttga
    31921 aacattagag tttacagggt taggatttgc gacaaatgaa ttagatgctt acatcatcaa
    31981 agatattagg ccgagtgggc agcccttaaa aaatgttctt gatcgattga ttgaatttac
    32041 tgaaggaaat tggcgcgttg gtcacgtaga agcaatgtta ccaacagtaa ctgcaacttt
    32101 ttactatgtc tctgtaaaag aagcgttgaa agaattgcaa accttaggta tggaatttgt
    32161 ctttaggtgt tctttgaatt ctgatggaat aaaggataaa tggatcgaag tatatgaaca
    32221 aattggcgaa gaatcgaata cacgtttcgt atatggtagt aaagcattaa cagttgtaag
    32281 agagatagat agaagctcaa tctcaacttc aatgataggt cgtgggcgag gcgaagaggt
    32341 tggtgacggt tacggtagaa gaattgaatt cactgatgtt gaatggaaaa agtcgaatgg
    32401 tgatccttta gataagccta aaggccaaaa ttggcttgaa gatccggaat caactcaaaa
    32461 gtatgggata ccacaaaaag atggatcaat gagaaaacga gaaaccgtag tagtgtttga
    32521 tgatatagat gatccaacag atttacttaa aaatacttat tcaaccttaa tcgattctgc
    32581 tagaccgtta gtacaattca aagctgaagt cactggagga gatgtgatag gaaatacagt
    32641 gactattcac agatacgata aaggttatca ctataaaact cgtatttata aaactacatt
    32701 caatcggctt accggtcaaa cgaatatcga actaggggat aatttaacac aagatgttag
    32761 aaaacaaacg gcttctattg tcaataatat taatagttta gaatctagca aaatgacatt
    32821 ttacgaatcg acagagattg gaaaatatca agatgacatt atgcgaggtg caggagataa
    32881 tggcggttct atttattggg taaatggaat tgaagctggt gttagtgata gtagagaaat
    32941 ctatgaaact gtttatatgg atggacctaa cattcctaga tcacgctttt ttatggtcca
    33001 aaataactca gggatatctt tcaaacaatg taaaaaaggt gagtggcaaa caattcaaga
    33061 tgtacacaat ggtgatagca cgaccgcttg gacgttggat ggaactttca atgctaattt
    33121 tattcgcgca ggcattctgt ctggtatcct cgtgcaaggg gtagctttaa aaacatttga
    33181 cgataaagat ttccaattag tggcagaggg gggagaactt tcttttgaaa aacaagtcaa
    33241 gtctactgga ctagatgatg tacatggaga acgcttgggc tcaattgtat ctacttatgg
    33301 ttctaaaggg attaacggct ttgctgtctg gaaagagcca aactatattt tttccattaa
    33361 tgctggggac ggtggcgatc gagggaatcc tatttttcaa attccagcag atgttactgc
    33421 tgataagcgc aaatataatc tttatggtga cggcaatttt tcagaaggaa atataaccat
    33481 agatggacgg ctagatgtaa aagaactcta tgttaacggt acaaaaattg acactaatgg
    33541 aggaggcaat tccggaggga acgataacgg ttggaacgga caatacccac cagaagtaac
    33601 tactgatcgg gataaacgat attggcaaat ttgggcaacg gcaataggtg ccggttttac
    33661 acaacaagct gctgcagcct tacttggaaa tgcacaagga gaatcagatg ctaatccaac
    33721 cgctgatgag ggcaatggcg caccagggtt cggttatggt gtatggcaat ggaccgattc
    33781 ttctggcgca actagcggac gtgtctacat gatcaattta atgacaaagg ctggcatcag
    33841 tgatgatcca gacacaatca cggcacagtt taaattgttg atgtggcatg caccaaatgg
    33901 tcaatggatc gcaactagcg cttatcctta cacatggaca caattcatga atctgaccga
    33961 tatcaacaca gcagcacaag cattcgtggc taactttgaa cgtccacgtg atccacatcc
    34021 agaacggacg gcatggtcac aagaatggta tgacaagttc aaagatttgg aaattcctgt
    34081 atcaaaagga tatatgaagc cgattgcgga tccaatcaca gtgacgagcg aatttggctg
    34141 gcgcacttct ccaatcacag gagcacaaga atttcataac ggtattgacc ttgtaaatgg
    34201 aaatcctaat acacctattt ttgcttcagc ggatggcgaa gtgattgttg caggtgatgc
    34261 aaattacttt gactggtatg gaaattggac agtgattaaa cacgctgatg gaatgtatac
    34321 aggatacgcg catcaaagcc gagtagacgt ctcaaaaggt cagaaggtaa ctgctggtca
    34381 gcaaattgga ctgatgggga caacaggacc atccactgga gaacatcttc attttcaatt
    34441 tatggatgag ttttatccat cttcttcagg tcatttccac aatgcaagag actacatcga
    34501 tttctaaagg agggatagtc atggcagaaa cgcagcataa aatggtccta tccaccaccg
    34561 aaccaaacaa cggaataaat ttggttcgaa ttcggcaagg ggatgtttta acgcaaaagt
    34621 tcgttgttga agtggtggaa catggcaaac taaaaacatt cgagggccta gtgccgtttt
    34681 ttattaatac aacaaaattt ggcgaaaacc aacctgttga acaaaaagta caagaataca
    34741 gtccagcgca agcaaggctt gtttacacct taagtgagcc tgactggcaa tggggtggtg
    34801 aaaacaccgc acatttcagt ttccgatcac ttaatggtga tggaacttgg agtgaacaat
    34861 ttagcacaca ggattttacc tatcgagtca tttctggaat atctagaagc cagttacgtg
    34921 actctggcta tgtgtggaca tttgaggatt tgctaagaaa attcaaagat tacatggatc
    34981 agggcaaaaa tgactgggag cagtggttag aagataatcg tgaaatactg gaaaatatcg
    35041 atccaggtgg tatgatcatt aacattctga atgaagctaa aggcgactac gaatcattag
    35101 ctgatcgttt aaaccaaaaa taccaagtgc cagtcggcag ctcacaaatt agagaaacaa
    35161 cacgcttttt tgattacgac acgatgaaat acgttgacct agtgccgcgc aatttgaata
    35221 cggttgtcaa cagtgttaat aacagcaaat ttaacttttc tttcattaca gacattcacg
    35281 cagataatca caacttgcgt atagatggcg tcggttacaa agatgcttct tatttaagac
    35341 attggcgcgc aatccctcaa tttcaaaaat tagggaacaa aacagatgtg atgatttacg
    35401 gcggagacaa tatcgacggc ggacttggtt cactcggcag cgatataggc attattgatg
    35461 aatggagcgc gcgacattcc atgttaggca cgctcaaacg cttcactaac gcagcggtag
    35521 caggacaaga aaaaccggtt attatctgca aagggaatca cgacgcttgt ttcgaccccg
    35581 cgtggcgcaa gcgaaaggga atgttatgca acgctgactt cgaagagtat tggaacggtt
    35641 tgtacggtgg cgtgttgttc ccagataaaa acgcagcaat ttaccgtttc gatacttgtg
    35701 atttttatga aggcggcaca ggcgacaagt acgtggacgg ttacagcgat acagcgccgg
    35761 gagctttcag tgccaaacaa attaacgctt tcggagaatg gttagtgaac gttccgagaa
    35821 actatcatgt ggcgttagta ggacatacgc ctttaggtct cagcaagttt cccgttcgca
    35881 atgaaaacat gattagcacg ttgatagaag ggtttaaatc tggtagtccg gtaactatcg
    35941 attggtctaa attaggacaa ccgaatgacg gttcgttcgg cggattaaag accttcgcaa
    36001 tgaatacaaa aggcgccggc gttgtagtag gttatttctg cgggcattgg cacgaacaag
    36061 tcgaaggggc gtttggaaca gtaaaaatga ttctttgcga cgtcggcttc tctcatacag
    36121 ctagccaagt tgatacaccg gatgaattag cgttttataa aatagaagtt gacacagcaa
    36181 caagaaaagt gacaagcaaa ggcgtagggc gcgcgagaga cttcacttat aactattaag
    36241 gcggtgaaca attaaatgtt agattttcaa agcaaaccaa atatttttga agaaatgagt
    36301 tatgaagagg ctgtgaaatg gttattgcgt caagcggcta tccattacga cggctcggat
    36361 cacgacgcgc acgtcctagc tactgaaagc aacgcgggct ttgctacacc ggaaacagtt
    36421 atgcaagcgc gtgggcgttg gttacgcgat tataagctac cacaaaaata tccgaacatt
    36481 ttagacattc cgcccggcaa atacgcaacc aaagccggat ggggcgcaga caaccctgac
    36541 gggattgaag atgacagttt tgttgaaatg atggtattcg cggatcacga ttcgagaaaa
    36601 ttaatcgtcg cttttgctcg ttatagcggc gaaatttaca tcaaaatgac acataacagc
    36661 gaaccggtag agggttataa ctcgttaggc tggcggcgcg tttacactac ttctgttctt
    36721 tttgaaggcg aattaagaaa aggacaatca gtcaatctgc cagatgatac tttccgttac
    36781 caaacgcttc gtatccacta cacagacggt gacggagact ttgtagaaga agtgaaacgt
    36841 caaagatacg cgcgaattac aaaagcgaat ttatggaatg gagacgcggg agtgacgttc
    36901 atcgaatttg agctgacaat cgaagcgcga aagataacga tgtcaaacgg cagagcttta
    36961 gatatttcat ccggcaatgt ttccaatccc gcaatgagca acaacgtgaa aataactaag
    37021 attgaaggtg tgaaataatg gcgcatgtca tcaaaaaagg ctctatcaaa gtacctacac
    37081 agccgaaaga ctacgatttg caagcaacgg ggcttgtatt taaatcatac gataatcaaa
    37141 tagcgttaga gttcaacgtc gaacaacaga acggcacacc ggcggacttg ctaggagcta
    37201 acttgcgctt gttgatgttt atctatgatg aagtagatgg aatgatcacg aaagagccaa
    37261 tcccttttat cacgaaaaac ctcatcactg aaagcttctt gaatggacag gtcgtatata
    37321 tcttgccaga agcgatgaaa gcttataacg gtatggtgga agcttatgtt tacatcgagt
    37381 atccagacgg atcaacaagt gataacttag gcttcacctt ccgtatgaag cgttcagcaa
    37441 tcgacggact agcgcaagat aaagcagact actttattgc agactttcaa caattacttg
    37501 atggagtcaa acaagaagca acagatgctg ttaacgcagc attaacaaat gtggattcta
    37561 cgattgaaaa ggcaaaccaa caaataaatg aatttgtaga gggagccaca caagcaattg
    37621 atcaaactgt tgacgaggta acagagcaac tacaagctac tcaaactaag attgataccg
    37681 tttctcaaaa cgttacatcg gcacaaaaca atcttaaagc agttgaagac aagatgaatc
    37741 aaaccaatca gcaaatcggc gacctcggca agctgaaaaa gatgtacagt aacagcatcg
    37801 acttcgggaa ttatgattat agtgggaatc cgaatttaat gtccaaacta aaatcgagcg
    37861 attttaacgt tggttaccac gggtcactaa cttcggataa cgaaaagcta cattttactt
    37921 ctgatggtac aggaagcatt attatgttta cgcgtattaa tacacctcag cttgctagtg
    37981 ggaaaaccta tactctgagt gcgaaagttc gatttgatga aggaactaca ggagctattg
    38041 ataaattacg tttggtgtat cgtacatcac caggagaaaa gatattattg gaagcaaata
    38101 gtacaaatat tacaacagat gatgtaggga aagaaataac aatcaaaggt acagctaacg
    38161 ttaattatca aatcacaaat ttagatcgat tttatatgag tattagcttt gttgacaggg
    38221 ataaaataaa tggcggattt aagttgtacg acatcaaaat cgaagaaggc tcaacagcca
    38281 ccccatatca gccaaactta cttgatgacc cttactggct aggtaaagcg cctttgggtg
    38341 agaatattgc taataagtct gttacgtttc caatcaaatc tagcgcctac agcctatacc
    38401 aagctaacat ggaagaagaa tttgttttag gtcaaaccta tacaattacc atgaaggcaa
    38461 ccaaaccacc tatccaaacg ttcattgttt acaacgagga tagtggagat tatagatatg
    38521 gtaacttaga gccagtagaa gggttagttg atacatgggg tctgactttt acaccgcaga
    38581 aggttggagt taactacccc aaacggttaa ctattatcca gtatccccaa tcaacaacag
    38641 gtgcatgtca aattgactgg ctcaagatcg aaaaaggcga cacacgaacc ccgaatatta
    38701 gtcagtttaa atactttggt gaaggcttga aagacagtaa caatcccaat gattacagtt
    38761 gggatgtcac acctgaatat actgaaaaag gcttgaatga tgctgttaat gtgtatgatc
    38821 ctcagagagt tgaaggtttg aaaaactttg ccgatggtat tcaaatagca ggagataaag
    38881 tgatcagtga aaatgattgc actgtttata cattaactaa agacaacagt caatcgttta
    38941 tcgatgggta tgcaacattt attaaacacg gaaaagaagt tattgtaaat ggtacagtaa
    39001 agttcaaaaa agcttatgct tttggtgtac cacttgatga tgaagtccca gatgaatttg
    39061 tcgcaaaaat tgttcatggc atgcttacag gtccgtcagg aacaagtagt gtatcaaaag
    39121 cgatgtacgt gcgaaaagac ttaggaacaa ttattacgaa tagcgaattt gctgcaaatg
    39181 aatggtttac attccatggt aattattggg taggggagga ataaaaatga aaaatatttg
    39241 gaaatacgga cgaacaggtg gagagtatgc tggacaagtg cttgatgata tggttatgac
    39301 tgttccattt accgatgttc caccactcga aggaattcgt gctgatgggg aaccactaac
    39361 gattgctgat cagatgtttg atcctaaact gaaccaatgg attgttttag tgaatgtact
    39421 agatcacaac gatttaaaca atctcaaagc gatgtatgag tcgttagaaa atgagaacgg
    39481 cgatttaaaa cagctcaacg ccaaactcat gctaagcgat gtagcgatta aacaggaaaa
    39541 tactgcattg aaagaaaaag cggatggttt agcacaaatc aattcaaaaa cgatgcttgc
    39601 ttcgttccaa aatagcaaag acattgcaga aattaaagag caactaaatc cagcttcaaa
    39661 gggaggtgag tagtatgttt agttttagcg atgtgaaaat gatgtatgat tggggctgtt
    39721 ttactgacga tcaagttcga ctattcgttc cactatgcat tacagacgaa gaagcagaaa
    39781 aaatcattaa taaagataag agcgcatctt aagtgatgcg tttttattta aggtaaagga
    39841 gttgtcacat gattaattta ggagaatggg gaacaatcgc aggatcaatc actgcgattg
    39901 tttctttgat tttattagta ataagaccag tcactgcatc tttctcgaag attactgaaa
    39961 ctctttcaaa agtaagtcac aatttagatt tgctgactaa agatttagaa tcgagcaaat
    40021 cagatcgatt gatgattcat gaagaactaa agaaacacga tgaaagatta gatacacata
    40081 cagaaaaatt ggtagaacac acacaacaaa tcaaaacttt atttagagaa agatctcggt
    40141 aaaaaagaaa ggagttaaga agaaatgatt ttacccgata agtattatca agtcattaaa
    40201 tggacagttt taacagtatt gccagctgca tctgtattag ttgcaacact aggcaaagcg
    40261 tatggatgga atggaacaga tatgacagta ctcactatca atgcagtagc aacattttta
    40321 ggcgttatca ctggcgtgtc ggcttataat ttgaaaaaat aggaggaaac aaatgaaaaa
    40381 gaaaattact attactgcga tgagcctatt aacggctctt tttttattgc caattaatgg
    40441 gtttgcctat acgattaaca atgaatttaa tttaggccca aacgaaggta gctcacaagt
    40501 agcaaataat cagtacattt tactgcatga aacggctaat gaaacagcaa caggacgcaa
    40561 tgaagcgcag tatatgcaac gttcatggac tagcgcttat actgcttata ttgtgggaga
    40621 cggcggaatt gtttatcaag tcggtcaacc tggttatgta cagtacggtg ctggttcgta
    40681 tgctaatgca aatagtcctg tgcagattga gttacaacac acacatgata aagcaacgtt
    40741 tgagaaaaac tacaaggcat acgttgaatt agctagagat tcagcaatga aatatggtat
    40801 tccacttaca ctagacacac cgtataatca accagggatc aaatcgcatt tatgggtaac
    40861 acaaaatatt tggggcgatc atacagatcc ttacggttat ctttctgaaa tgggcgtaag
    40921 taaagaaaaa ttagcctatg atttggctca tggatttacc gatgaaaatc cgacaacttc
    40981 agatgataaa ccagtcattg atccaactcg agcaggtgct gcaaatccta ccctgacaga
    41041 tggaacaaat tacgcccaca ttgatcagtt cggagaaatc gaaaacgcaa acttacatgt
    41101 ggctggttgg cacatcgcta actataaata cgagtatatc ttcattatgg attacaatac
    41161 tgggaaagaa ttagctcgag taaaagctga tggaatatat agaccagatg taaatcaagc
    41221 ttataatact ttaggaaatg ttggctatca tgtatctttt aacatgcgca acttccctaa
    41281 taagaaagtc tatgtaatga tgcgggcaac gaatgatcca gaagggaaca ctaaaggcgg
    41341 agcacaagat tttcatgata aacgctggta tttaaatatt ccgcaacgat aaaaatagcc
    41401 cctcgttttt gaggggaagt acataatgca aatttgtcca taacttattg attatcttgt
    41461 aaaaatgcgg taatttaata gttagtgaga agaggtgctt aattatagtt aaaaaagagg
    41521 aaatagaagc agcatgtaac caagcaaatg ttaggtttat tggagaagct ttatctggat
    41581 atcctaagta taaatactta cacaactggt tacaaagtaa aagtttttat cttaataaag
    41641 aaagatgcat gcctaataaa aattataggg tttataaaag aggaactatt gtatatgttg
    41701 attttggggt gaacgtgggt tatgagttat ctggtaatca ttttgctatt gttttaaata
    41761 ataaagataa ttcgaaaaat ggtgttgtat gtgtagtacc aatctcttca aaaaagaaaa
    41821 gtaattatgt atctgtagga aaaatattag aagttgctag tattaaacaa tttataagcc
    41881 aagctgataa attaaaagag aaacttagga taattatttt actttcatta aatagaggca
    41941 tcattgatga aagcgatttg aatagtttta ttaaagatat tgaagctaaa ggtgaaaagc
    42001 tttcagtgga agaagtagaa aaacgagctg taaaacataa tttggtttgc gagacaaggg
    42061 aacaagtaat taaagaaata aaatcattat ctgaagatat ggtaaaatat cagaaggtgt
    42121 ttaattgcta caagaaatat acacaagaat cctatgtaat gccacttaat atccaaacga
    42181 ttagtaaaaa tagaattaga agaattaaca agtttgatcc ttctggaaat atgaaggcac
    42241 ctggaaatgt cctcaatgaa gtagatgaaa gtttaaaaaa atattttaca aagtaaacga
    42301 tatttttgtt gacatttttc tgttcgttag ttaccatgta gttatagaag gacgtaagcg
    42361 cctattttta atgtacacat cgggacgtat gcgcccacat agaaacatag ttgagagaca
    42421 tctatatggt gtctcttttt ttattaatat gcaaaaaagt ctagtttttt caataatatt
    42481 tatgtaataa taaattagcc accacaacaa agaatgaaac ccattattat ctagtctatg
    42541 tccattcttt ttgtttgcag tagttgtgat ggctttccgt actcttagct cagttggtta
    42601 gagcagacgg ctcataaccg tccggtcgta ggctcgagtc ctacagggta catactctaa
    42661 gtgatttaaa ttgcgaataa aatataaaaa tgactcccct tttgactccc ccaagcagta
    42721 tatggatgtc tctaaaagtt gtttttttag ataataaaaa gctgataata agcgaaaaac
    42781 acttaaaatc agctttaaac cctattctcg aaatccggcg aaccggctaa taccggcgcg
    42841 caggttcaaa tcctgtacct tccttatatc aacgattaga ggttgtgaag aagctgtcct
    42901 gcatcaagta ataagaacgg ccaaacaaaa atagcttttc ctattttttc gtttgattgg
    42961 gcttattacc gcagatgcaa gcttttgaac accgtttgtt cggaataagg ggctgtgaca
    43021 agacttttgt cacagcccct tattgttgta cttttaacct acaatatttt ttgattcccc
    43081 ctgagtacta ttttttataa atgctagtaa atcaatcttc tttggccttt ttttgataaa
    43141 aaaaatacta gatgtaagat aaaagtgtaa aaacgatttg ttttaggagg ggaaacgatg
    43201 tgtaccgcaa tcacttatgc aacgaaagac cattattttg gaaggaattt tgattatgag
    43261 atgtcttata atgaagtggt cactattact ccaagaaatt atcgtttcga ttttagaaag
    43321 gtaaaaaatt tagataaaca ttatgcaatg ataggaatag cagctggggt atccaactat
    43381 ccactctatt atgaggctac aaatgaaaaa gggttgagta tggcaggact gaattttcct
    43441 ggcaatgctg attataaaga attacaagaa ggaaaagata acgttgcacc atttgagttt
    43501 attccatgga tactagggca atgctcaacc atagacgaag ctaaagaatt attggctact
    43561 atcaatctag tcaatatcga ttttagtgaa aaactaccct tgtcaccttt acactggtta
    43621 ttagctgata aagaaaaatc gatagtaatt gaaagtatga aagatggact tcatttatat
    43681 gataatcctg tcggtgtgtt gacaaataat ccgccatttg attatcaatt atttaatctg
    43741 aacaattatc gctcgctatc aaatgg
//
LOCUS       DANKMN010000014        42309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000014 DANKMN010000000
VERSION     DANKMN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..1563)
                     /locus_tag="PW224_000783"
     CDS             complement(<1..1563)
                     /locus_tag="PW224_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2907491.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDH"
     gene            complement(1567..1977)
                     /locus_tag="PW224_000784"
     CDS             complement(1567..1977)
                     /locus_tag="PW224_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2907492.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(1974..2897)
                     /locus_tag="PW224_000785"
     CDS             complement(1974..2897)
                     /locus_tag="PW224_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL2907493.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2894..3772)
                     /locus_tag="PW224_000786"
     CDS             complement(2894..3772)
                     /locus_tag="PW224_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL2907494.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3759..5096)
                     /locus_tag="PW224_000787"
     CDS             complement(3759..5096)
                     /locus_tag="PW224_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2907495.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5099..6202)
                     /locus_tag="PW224_000788"
     CDS             complement(5099..6202)
                     /locus_tag="PW224_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2907496.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6276..7784)
                     /locus_tag="PW224_000789"
     CDS             complement(6276..7784)
                     /locus_tag="PW224_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2907497.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7750..8442)
                     /locus_tag="PW224_000790"
     CDS             complement(7750..8442)
                     /locus_tag="PW224_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2907498.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8464..9438)
                     /locus_tag="PW224_000791"
     CDS             complement(8464..9438)
                     /locus_tag="PW224_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2907499.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9738..10610
                     /locus_tag="PW224_000792"
     CDS             9738..10610
                     /locus_tag="PW224_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2907500.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11068..13266)
                     /locus_tag="PW224_000793"
     CDS             complement(11068..13266)
                     /locus_tag="PW224_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2907501.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13498..14490
                     /locus_tag="PW224_000794"
     CDS             13498..14490
                     /locus_tag="PW224_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2907502.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14550..15971
                     /locus_tag="PW224_000795"
     CDS             14550..15971
                     /locus_tag="PW224_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL2907503.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            15964..17463
                     /locus_tag="PW224_000796"
     CDS             15964..17463
                     /locus_tag="PW224_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL2907504.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17502..18896
                     /gene="uxaC"
                     /locus_tag="PW224_000797"
     CDS             17502..18896
                     /gene="uxaC"
                     /locus_tag="PW224_000797"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2907505.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19055..19738
                     /locus_tag="PW224_000798"
     CDS             19055..19738
                     /locus_tag="PW224_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL2907506.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19740..20576
                     /locus_tag="PW224_000799"
     CDS             19740..20576
                     /locus_tag="PW224_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2907507.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20587..22341
                     /locus_tag="PW224_000800"
     CDS             20587..22341
                     /locus_tag="PW224_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907508.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22617..22958)
                     /locus_tag="PW224_000801"
     CDS             complement(22617..22958)
                     /locus_tag="PW224_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2907509.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23026..25197)
                     /locus_tag="PW224_000802"
     CDS             complement(23026..25197)
                     /locus_tag="PW224_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2907510.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25366..26265
                     /locus_tag="PW224_000803"
     CDS             25366..26265
                     /locus_tag="PW224_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2907511.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(26336..27193)
                     /locus_tag="PW224_000804"
     CDS             complement(26336..27193)
                     /locus_tag="PW224_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2907512.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27178..29880)
                     /locus_tag="PW224_000805"
     CDS             complement(27178..29880)
                     /locus_tag="PW224_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2907513.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29893..31182)
                     /locus_tag="PW224_000806"
     CDS             complement(29893..31182)
                     /locus_tag="PW224_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL2907514.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31332..32378
                     /locus_tag="PW224_000807"
     CDS             31332..32378
                     /locus_tag="PW224_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2907515.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32380..34533
                     /locus_tag="PW224_000808"
     CDS             32380..34533
                     /locus_tag="PW224_000808"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL2907516.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(35006..36457)
                     /locus_tag="PW224_000809"
     CDS             complement(35006..36457)
                     /locus_tag="PW224_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2907517.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(36454..38178)
                     /locus_tag="PW224_000810"
     CDS             complement(36454..38178)
                     /locus_tag="PW224_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2907518.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(38171..38785)
                     /locus_tag="PW224_000811"
     CDS             complement(38171..38785)
                     /locus_tag="PW224_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2907519.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(39130..40587)
                     /locus_tag="PW224_000812"
     CDS             complement(39130..40587)
                     /locus_tag="PW224_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2907520.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(40628..41548)
                     /locus_tag="PW224_000813"
     CDS             complement(40628..41548)
                     /locus_tag="PW224_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2907521.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(41560..>42309)
                     /locus_tag="PW224_000814"
     CDS             complement(41560..>42309)
                     /locus_tag="PW224_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2907522.1"
                     /translation="GWENFKFLFSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMS
                     ELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNE
                     PKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQL
                     IPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTA
                     AAGLYQSVVGFVLLMITNTIARRFDSDSALF"
BASE COUNT        12409 a         8660 c         7526 g        13714 t
ORIGIN      
        1 atgatctttt ccatcatact tgaaaaactc aaaatcagca taacttcctc tttttttatt
       61 tagatcatgc actgctatcc cgataaaatt tccagtaaac cctcctgaga gtatacacat
      121 gtttttttgc ggacctactt tcttccattc ttcagttgat acgtctttca aatgaaactg
      181 tgctgtctct ttctttacag tgactttcaa aaaagtcttc ttttctgtga gtggaatcgt
      241 ccaatcttct atttgtgcta ctccgttttc tgattctatc atacgtaggc attttccctt
      301 gtcctcattc caagtaacat aacaataaat ataattggat tcatttaagt acagtaccag
      361 tccagccatc tgattaaaat tattaggcat gtattcaaat gctgtctccg catcaaaagt
      421 aaaatccgtt tgccgaatag ctaacaaatg atgttcgaag gtagattgaa gagattcacc
      481 ggaaatcaga cgtaaaaaac ctggtctaca ttttgtatca caccaatcag catttggcaa
      541 aatgcgtctg gcattccagt tcttctttag ctctccttca aatgtatcca taaactgatg
      601 atctttttct tgttgtacct cttcatttgt ctcaatcatt atttcttcca tcggagttgt
      661 ctggctatct ttaagtctta accaatcgtc ggacgtccaa tacacttctt gaatagcggt
      721 ttctcttccc aaaatagccg caccgtttaa tggtctggta caaagatatg tcatgtacca
      781 tcttcctgac ggggtttgta ctaagctccc atgtcctgta cattgcaatg tagaagcagg
      841 tttgtcacta gctgtcagca taggaaaacc aggatcaagc tcgtaaggac cagtaatatt
      901 ctttgctcgg caaactgtaa ctgaatgtcc tcttcctgta ccgccttctg ccgtcatcaa
      961 ataataatac ccgttacgaa aatacaaatg tggtgcctcc gttttcgcta attctgtacc
     1021 tgcaaatatt ttatagatgg gaccatcaag cttcttcttc tctgcatcat attcttgtaa
     1081 tacgataccg ctggatttat tcggtgtgtc cagacgataa tcccatagtt cattgacaag
     1141 ccattttttt cccgtttcaa gatcatggaa caaagaagga tcaaagccgt tactatttaa
     1201 ataaattggt tcggaccaag gacctgtgat ctcagatgca gtgatcaaat aattgtggac
     1261 atctttaaac ggacggcctg cacttttgac atctgtgtaa acaagataaa acaatccatc
     1321 agcataactg atttgtggcg cccatatact tccatttgta ggatttcctt gcaaagatac
     1381 ttgagtggtc aaaatatccg ttgtatgtga ccagttgact aaatttttcg actcatatac
     1441 tcggattcct ggaagccatt caaaagagga gacaataatg taataccgct ctttcactct
     1501 taatatatta ggatctggat tgaatcctct taaaatagga tttttcactt tgatagtctg
     1561 catgattcag gctccttttc tcattcttac tcgatataaa ctgaatgccc cagctgcgaa
     1621 ataaaaaaat gcaaaaggaa acgcaataaa ctgagtgcca atcaacaata tgaatcctgt
     1681 tataaaatac aaaccagtgg cagttgttaa gtattttcct ttagcagtca aaaacaatgc
     1741 tactgctaaa aaagcaagtg aaataaacaa cacaacggca aacagaccag tcatgtcctg
     1801 taataatcca aatgtatttt ctaaagacaa agttgaatga tctacagcaa gcgactgata
     1861 acttttttta aactctgacg cagacatatt acttaaagta actgctaacc cgccaaagaa
     1921 aatcaaagat agactgccac cgaccattcc tagcaatagt tcccattttc ttttcatttc
     1981 tgaaccccct gcttttctaa atgtatccat tcaggtcttt tgatcgatgt ttcataattg
     2041 ttattacgtt cagagaattt gacagtctgt tcatttttct tgtcatccca attatcccaa
     2101 ccagttgggt gaacatgccc acctatctta cacgcttcaa ataagacatt tgcatacgga
     2161 cgccacggtc ttcctaaata gacgtttcta gtatcaggtt ctgcagtcaa ggtacagctt
     2221 tcaaaacgat accctgtttt ttgatttttc ggcgtagatg ctgctgtgat gtatccagga
     2281 tttttactgg ctaagctatg gatatgacac tgatgaaaaa cggcatctgc cccaccaaat
     2341 atgaaatcca ccgttccttc aatccagcaa tattcatagg tctgacgaca atactcagga
     2401 ttggcagaag ccggtgtaca aaaaggagta ccatcttttt gcaaatcagg caacggaccc
     2461 gtgcataagg tatcctgata accagatagt cgacagtttt ttaaagatac gcgatctcct
     2521 gaattgaata aagcaacagc ctgtcctacc ttttctcctg gacctgctgt attttcgatg
     2581 tgaagatttt ctaatacgac attcgatcct tctaagaaaa aagtagcact tcggaaagtc
     2641 cctaactggc taccgtcaag atactcctgt tttgcaaaac gattaccgga gataactact
     2701 gttcctgctc ccacaagttt aaaattggat aatctgcttt ctatatattc atcatatctt
     2761 ccttctttta ctatcaagac ctttggatgg ctatccggca ggcctgccaa ataatcgatt
     2821 ccttcttgga tagtttcaaa atccgtcggg gtatctatag cattctttcc aattatcagt
     2881 ttgtctttca tcctcataaa tgctccctta tgaattgttt ccaaatattt ctcatttctt
     2941 gtgtttccat atgaccgcct gttaaagaaa aagaagaaaa gttttctgga ctacccattg
     3001 attgatactt tttgtccagc ccttttctca gcttcagtac gccttcgtcc aagcacatcg
     3061 tatcgttttt tccaactaag ctaagacgag ggcgcggagc gattttttct tggattgcta
     3121 acgttgtata agctgtcaat agatttggca catagtaata aaacccatga tgatctaggc
     3181 ggcgatgtag caaaagtatt tcgatgtcca cctgtgccgc tagatcaata caaactttga
     3241 cacgagaatc caatgctgtc acccaccagc ttaataagcc tcccatagaa agaccaatcg
     3301 ttgcgagccg ttgagtatct gtgtcttccc ttgtctccaa ataatcgatc aagctgatca
     3361 catcataaat acgcattccc cataacgttt ttcctgttaa taaaaattgt ttgaacaact
     3421 cgctttcaga aatacctccg cgttcttcaa atccccaagc atcaatcgac caaacagcat
     3481 atcccattcc tgtcagtact tctgcaaaag atggagaagc taagtaagga gcaccatgga
     3541 tcaattcact tttcccgact gaaaaatctc cgccgtgaga atgcaggtaa aggaccggcg
     3601 gaaacggtcc ttctttttct ggataagcat agagggctgg tactgtttcc aattcattta
     3661 agtacaattg atagtattca agcataaaac cgttttgttt ttctcggtat aatcgttttc
     3721 cactaggggt atgtctgcta gatggagcac ctaaaaaatc attcaactgc ataagcaaag
     3781 tttttacgaa caaccagttc atccatattt tctgattgat ttgattggaa tttctcaata
     3841 ctgatttctt tagtattttc taatagaaat gctggtccct cgtgattttc aatcgacatc
     3901 tggttaaagg aaaccttacc cgcaaagcca atataaaatc cttgtctgct catttcctct
     3961 attcctgtca tcatagcagg aacaccggga actgcatctt gggccatcga gacatcgata
     4021 ttgtcaaatg tgatttcttg tatgaattgt tccgctaatc cataaataaa acctgcagaa
     4081 gcatggacat tccttgccgt gatattcgaa aaatggatcc ttctgaatgc tggtgtccgt
     4141 tcatctatcg gataagcctt tttttcccat acatacggtt ctttccctcg cggaccacag
     4201 aaataataaa gattcagtat aaatggacac atcacattat ccatgactat attggatact
     4261 cgaatatctt caacgatccc gcctcgacct cgtctagatt tcaatcgtat tcctcgatca
     4321 gtttcttgga agatacaatt tgaaatggta atattacgaa tactaccact catttcactt
     4381 cctaatacga caccgccatg tccatggacc atcgtgcaat ttgtgatcgt gatattttcg
     4441 caagcgatcc tttcgtacgt atcttcagtc cctgccttga ttgcaataca gtcatctccc
     4501 acgtcaatat ggcaattact aatacgcaca tttttgcatg attctggatc aattccgtct
     4561 gtatttggtg aatccgctgg gttcaagatc gtcaagttat caaacgttgc attactgcaa
     4621 agaatcgggt tgatcgtcca acttggtgac tggatcaatt taatatcttt cacagtaatt
     4681 cgatgacagt tatggaaact catcaattta ggacgaggat aagcgagatt atctggttca
     4741 tttctaaaag tatgccacca tttttttcca tttccatcta atgtaccgaa accagtgaca
     4801 gagatgttct ctacattttg agcatagata caggaagcat aaacttttcg atggactcct
     4861 tcccaacgtg aatgaacaac aggataatct tttggatcgt cagagaattt cagaaccgct
     4921 cctgcgctta aatgaagttc gacattgctt tttaaaaata aagctcctgt tagaaattca
     4981 cctgctggaa caactacggt cccgcctccg tcacttgctg cagcatcaat ggcttgttgt
     5041 attgcttcag tattcaattc atcaattgat gcgccaaatg tcaaaatatc atacatattt
     5101 ataatgcctc cacttcaatc aatgcttgca agaatgcacc aacgcctttt tgatcattac
     5161 aaataattgg ttcactgatg tagtaagcat atgaaccatc tcgctgatct gctccgccta
     5221 accctgctac ttgacagttt ttattcaagt tcagccagcc ttcttttgtt tccagaacaa
     5281 attcatcaag taatccttga tgtgatttct tcacttgtgg aagccattct tctttgttca
     5341 gcagtcccaa acgtattcct ttagccattg cgtaaacaaa catactactg ccggaagctt
     5401 ccagataatt tcctttccgt tttgtttctg ttgttacttg ataccatgtc ccgctgtttt
     5461 cttcttgata tggaagtaat gcagccagtg tcttttctag aatttctttc agcaaagaac
     5521 aatctgtttc ttcatgaagt aattcatagg tatctgctgc agccatcaaa taccagccaa
     5581 tcgatcggct ccaaaaatga tgggatagac ctgtttttgg atcagcccat ggttggacct
     5641 tcttttcatc ccatgcatga atcagcaaat ttgtttttgg atcgaataga tgtttataac
     5701 tgatttcaaa ctgtctcaaa acatcttggt aatctttttc ttcactaaaa ttctggataa
     5761 attcagcata aaaaggagaa cccatatata agccatccaa ccaaatttga taaggataaa
     5821 tttctttatg ccaaaaagct ccttcggatg tgcgtggatg gtttgccaac tgctgggaaa
     5881 ggagttctgc tgctttttta tatttttctt cgcctgtttc tttgtacaat aaaaagaaaa
     5941 gctttcctgt atttacatgg tcgatattgt attcttcaac gctgtagcca cggatcgttc
     6001 catcttcttg cacgaaatag tcgatattgt cttttatgaa atcaaaataa attttttctt
     6061 gggttcgttc ccaaagtaat tggaaccctt taagtacgac gccatagtca taagaccatt
     6121 ttcctttata ccacttttct tcatataagc gaggtgttct ttccatgata ctttttgcta
     6181 atttctttga ccagttttcc attatttgct ctcctttaaa aattaaaata ggatttggga
     6241 caaataccaa gaagtatttg tcccaatcgt ctatattact gtgccgctgc tttgtatgct
     6301 tcctcgtact cttgtgtgat tcgatcgcct ccttgtgctt tccagtcttc tacagctttt
     6361 ttgaatccat cttcgtcaag ctgtcccatg atgtattgca ccgtagcatc aatgacgatt
     6421 ttttcaattt ctgatcctac ttcattgttc gtcggtgaat ccaatggcac agaaggatcc
     6481 attacagcaa attcttcatt ttctttgatc aattgattag agaattcttt ttcagggtct
     6541 gtatctttga aagagaacgt cacttcacta ggacgagagc ttgataacgg ttgcacctct
     6601 tgttgccaaa gatccatatc gataaattcg atagaaccat catcattcaa ttcataatgc
     6661 gttccttcga taccgccagt catcaattta tacacatctt cttctaaaag atcatcaaca
     6721 aattgaagta atttcttcaa ttgtgcttcg tcttttactt gagatttggg aaatgctagc
     6781 aagccgccaa taccatttcc ttctgaccaa acatgataac cgccgccttc accatctgag
     6841 atcttgttta caggcactaa ttccatatca tcttgaatac cttgacccat cgtcttcaga
     6901 ttcttgatat ccaccattcc agtgtagatt ccagttcttc cttgtgcaaa ttgttcttgc
     6961 tgatcagtct tttgggtaac tgcaaagtct tgtgccaaat agccattctc aaatagatct
     7021 cttgaaaaat tcaaagcttt catatactct tctgtatcaa attctggagt gaatttgcca
     7081 ttatcgtcta cagcccagcc atttggtgct ccaaaataag aagtgaaaag tttaaagctg
     7141 gtataacgaa tatcactagg cgcacgatcg ccaaaaccaa ctgtgtcgtc cacaccattt
     7201 ccatcaggat cgttttctgt aaattgttta gctacttcat aaagctcatc catggtagtt
     7261 ggaacatcta gtcctagatt atccaaccag tcttttcgaa tgactaagcc agcacgagcg
     7321 tagtcttttt gtatcggtac gccgtacaag gcaccgtcga tactcgcagc ttcgattcta
     7381 tcttcagaaa tttgacttaa atttttatag tcttttaaat attctgatac atcccagaat
     7441 acgcctgatt ttaacgcatt gcggacagaa gagtttttca acatcgcaat ttgaagtgta
     7501 acgatatctc ctagttcatc agaagccaat gcagttgtta atctttcttc ttttgaagca
     7561 tcaggaatcc agttgaatgt cagttccgta tcggtttttt cttcgatcaa gttcacaatc
     7621 gtgtctgtag gcggtgaagc atgatgcaaa atatttaacc aagtgatttt agcttttcca
     7681 tcttctgtct ctgaagaact gttacttgaa ctgctgccgc atgcggcaag tgctagaatt
     7741 cctgcaaaac taagacctaa ttttacgagc gttgcttttt tcataaattt tctccttcac
     7801 tgttttaata atatttttgt tttcttctgt tttttctgta tatgagttct tccattccaa
     7861 atggcaaaat atagaatttg ctgtccacgt gactgcggaa aagaagagac tgccaccgat
     7921 aaaggttaaa acacctggca ttgtttggat gatcatcaga taaatcccta ccatacttaa
     7981 tatcaacact aatgagtaat gaagatgtga gaatccgaat aaaaatgcca ttttcatttt
     8041 gaaaaaatat cctttccaat cataatgagc catgattggg aaaatataga gtgctgagag
     8101 cacatatatg aacgcagcaa tatacaaagc gatcctaaga taccaattgt gtatgaaaga
     8161 aagatccaca agtaaaacaa tcccgatggc gccatatatc cagctcatca gcaaggtttc
     8221 acgatagttt tctttaaaat atgtgaagta cgttttcgtc aaaggaaacg tttcttttga
     8281 gcgcagccat tttctaatca ctgcaaccat cgcatacgat gcaggaccga aagtaaaaac
     8341 tcccaaacct gcgatgacta aaccgcccca cagaaaattc aaataaacca actgtaaaat
     8401 acgatttagc cttccgttta ttttatctaa tgactgcagc atatctttcc ctctctttct
     8461 atcttagtaa acgccgtctt ctcctagctt tttggcaatc ttattagcta aaattaccat
     8521 aaacaatcct acgacccctt taaacaatcc cattgctgta ctgaaactta actgtccatt
     8581 cttcaatcca gctgtataaa tataggtgtc gaaaatttct gcaacgcttc gattcaatga
     8641 attcaataac aagtacatat gctcgaaccc taagtctaat gtctgaccga tttttagaat
     8701 cagcaaggta atgattactg gacgaattgc cggcaatgta acatgccatg ttttgcgtaa
     8761 gcgtcctgct ccatccattt ctgcagcttc atacaactgt gtatcgactg cagttatcgc
     8821 tgaaaggtag atgatcgtag accagcccaa ctctttccag atcacttgtc cgatatatac
     8881 tgtgcgtgtc cattcagggg atgttagaaa actgattttt tcaaatccca ttgttgcaag
     8941 gaattcattc aatacccctc cgtcaacatt caaaaataca taagtaatcg aaacaatgat
     9001 tacccaagac atgaagtgag ggatgtaaat gatcgtttga atcccgtttt tcaatcgttt
     9061 gttcgttact tcatttaata gtaaagacaa aatgattggc atcggaaaaa acacgaagat
     9121 atttaatccg aacaagatca acgtatttct taataatgta aagaatgtcg gttctgtaaa
     9181 caaacgaatg aaatgtttga aaccgaccca aggacttccc attattccta aaaatggttg
     9241 ataatcttga aaagcaataa cgagtccccc cataggaaga tacctaaaaa tcaagaaata
     9301 aaggactccc ggcagaatca tcacatataa aaatttattc gttttgattc ctgcaattat
     9361 tttttttcgt cttctgagct tttcttcttt tttcgatagc gtcagttgaa atggttcttg
     9421 tttctttaca ctttccataa gcccctcctt agttttgtga atgtttgctg cttatgactt
     9481 catgatagat aactatctga attacgtata taatcatttt taccgtaagt aaaaacgctt
     9541 acatagcaat gatttttgtg ctatacaaaa cgataaagtg tattttttca caaataaact
     9601 acgcatagca aaccttctat ttttttctat ctgacactta gcataggaaa tgattatata
     9661 cgtaatcttt caccgatgat aaattgaaca tatagaaaca gaaacattta atttattttg
     9721 aggaggagcc tatctatatg aaaaagaaag tctctatagg agaaagaagt tttacacttt
     9781 tcaacaatac gtttctaatt ttgctagctt tgatctgtat cgttcctttt ttaaatatca
     9841 tcgctacatc ttttgcttct acacaagaag tcgtcgcgaa aaagtttatc ctttttccta
     9901 ctaccttttc tttagacgcg tatcgctaca ttctttctac gccgaccatt tttagagcac
     9961 ttgctgtttc gattggtgtt acaggtgtcg gaacgatcgt cagtatgtgt gcaacttcac
    10021 tgatggccta cggtctttcc agaaagtatt tgtttggtcg aggtttcgtc aactttcttg
    10081 ttgtattttc tatgttgttc agcgggggga tgattccaac gttcttagtt gttcgttctc
    10141 tagggctggt caattcttac tgggccatga tcctgcctgt ggctgttaat gcaatgaata
    10201 tgatcattat gagaaacttt ttccaagcct tgccggatag tttggaagaa tcagcaaaaa
    10261 tggatggttg cacagatttt ggtgtatttt tcaaaattat gctgccatta gcgttaccgt
    10321 ctattgctac tatctcgctt ttttatgctg ttacttactg gaacacttat atgactgcta
    10381 ttttgtatat caacgattca tctaaatggc cgatacagat tcttttacgc caaatcgtca
    10441 ttgtatcaag tgggatgcaa gcagaaagta gtgccgttga tgtgattcca ccagcacaaa
    10501 cagttaaaat ggctgttatc gttattgcaa cagtgcctat gctgattgtt tatccttttg
    10561 ttcagaaata ttttgtcaaa ggagcactcg taggttctgt caaagggtga ttatgtactg
    10621 aacgcaaaat ggtagttgtg tcagaagtgg tcagcttcaa gaaataagaa ggcatttacg
    10681 aaaattgctt cttaaatttt tgtgaaattt cggcttattt ttcgaagaat ctactcctgg
    10741 aacaccgtgt atcagaggtt gtgaaagaga cgttcagcct tgagcgatga taaaaactct
    10801 cacaaacatt gcgacgagta acccacagga gcaataagaa gcgattttga aaaatagctt
    10861 cccatatttc acaaaattat gccttattgt cgatagcctg tctcttgaac gccatttatt
    10921 cgtaaaaaag aactgtgaca agacttttgt cacagttctt tttgcgtgct tagccttgaa
    10981 atttaactat ccataaatac aaatactagg gttattctaa cttcttttat tgcttgaatc
    11041 agtccacaag aaaaacagcc tcttgattca caaatatttt tttcgatatt ctcccggcgt
    11101 aatcgattcc ttctttttaa agaaacgaat aaaattttgc ggatttcgat attgcaatct
    11161 ttctgcaatc tcttttacgg agaggttagt ctctttcaac catttttttg ctacctctaa
    11221 acgatgattt tgaacaaaat caccaaattt ttctcctgtt tcctttttaa atacattact
    11281 taaatagtta gggttataat gcaacctatc agcgatcaca tctaaagaca gatcacgatc
    11341 atattctgtg tagacgatat gcataatatt ctccgacaaa tttttaaatt catgatcagt
    11401 gattgcttga gagcattctg tgattggcag aactaattgt tctaaaacaa gtttttctag
    11461 ttctttggga tgataattgt tcagtgcttt tacataaatt tgtttcatgt tatcaaagtt
    11521 ttttgaatct actcccaata actgtcctaa ttgaacgagt tcattgatca agcggaccga
    11581 aactacttcc cgactcaaag gatttttatt ttgtgcaaac aatgcatcaa tcaattcatg
    11641 tactaaattc ttcacttcct gatttccaga acgaatggct tcaaacaatc gattttgttc
    11701 ttctatcgga tatctaataa gtgttttttc atgagcagca ggaacgattt cctgataaaa
    11761 aataattgaa cttggaccag tatttacacg ataatacagg gcttcttttg cccgatcaac
    11821 cgattgcttg ctttcattca gcgtttcgaa ccggttactg atgcctatac tgatagttaa
    11881 attcaagtat tcttttacac gtttttgaat gagtcggcaa tactccatga cttttttgct
    11941 tgcttctgca tcatctttat ccattcgata aatagtggct tgcatttctt cattcaaaac
    12001 gatcggcagc attcgatttt cctttggcac aatttccgtg atcatgttgt tgatcgctaa
    12061 aagtagcaaa tcacgctccc caccgtgact atcttctaac agatcgattt gaaccaaagc
    12121 tgtataatag cagtcatttt gagaactata accaaattgc tgcaaccgct gatctagttc
    12181 tcgcctttct actcgatttc taaataaact aagtacaaat agcgtttcca gctgtggctt
    12241 ttgggtaact aagtgtgcac ttaacgcttc tttttctcct atgatccgac tgactgattg
    12301 agcgaccaaa tctagttctt tcccactgaa tccttttgat ttaagattca gacgttcctg
    12361 tatttgagaa atcggacggg caaatctgtc cgaaaataag taggaaagaa gaccaatgag
    12421 tcctaccagt gttaaagaca ctgcaactag cccgattctc agattgcgga tcattgcgcc
    12481 aattgcacta gagtcaactt ctaatctgta aatccaatta ttataatctg atttcagtac
    12541 tatatacgtt ttatcttttt ctcttatcac tttcagctga ttgtaatcta gatccacgtc
    12601 cgttaataca gttggttgct ctttattttt tactgtctga gtagtaaaaa ggttgccaac
    12661 ttcgttttca atagaaagta tgtcttctcc actttcacca acaacttgtt ctattgtatg
    12721 tttctctatc gtagcaattc ccaatgcata tttttctttt tcaaacattg gcaaagaaat
    12781 caccatttcg attcctttgt ttttcttttg ccaaaataaa ttatttttgt tatttatgta
    12841 cttctgttga tacttttcaa cttcctcttc ggtcagttga ctcaacgatc cattgattac
    12901 tccccattta ccagccaaac tgactaaatc ataattatta tcatccataa caatcgtttc
    12961 tataaaattt agttctttcc tgatttcaga ataagtggag taatctgtat atgtcagcgg
    13021 atgcttgaat atgtccgaga aatttttggt cgaaccatac gtagaaaacg aatattcaat
    13081 cgttctaatt ttccattcaa cgttagtttt aacttgcctg agcaaattct ttttgtctgt
    13141 taaataactc gattcgatcg attcataggt attctggtaa atgaataaac taaatcctat
    13201 gacaagtacg gctcctaata aaaacatcgg taaaaatatt ttataaaata ctgtactttt
    13261 cttcattttc ttcctctcct ccttttaaaa taaaaggctt tcatttttct gtaaaattga
    13321 tactcatcta attaaatgag cattccccgc tttcaagaat agcataatta aaaataaaaa
    13381 tccaagtaat ttttaacgtg tgcaatcttt tcatatcaaa gatctattga cttatcgtag
    13441 ttatcgtaat atgatgaatg cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg
    13501 gtaacgatca aagatatcgc taaagcagct ggcgtttctc atacgacagt ttctcgtgct
    13561 ttgaataaca gcagtttaat aaaaaatgaa acaaaacaaa aaatacgaca actagctaag
    13621 gaaatgaatt attcgccaaa ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta
    13681 atcggactat ttttctcaag tttgcataaa ggaacttcat caaatttcct agcagattcc
    13741 attgcaggca tccattcagt attagacaca aattacagtt tatctgttga aagtatcgat
    13801 gttgttgatt taacagaaat caacctgcaa cgctacgatg gactgatcgt tatgagtcaa
    13861 agtgatgatg atcagccttt catcgactat ctcaaagaaa aagaatttcc tttcgttgtt
    13921 gtcaatcgtc acatcaatga tccagcggtt atcaacgtca ctgccgatga tgcggccggt
    13981 gttatgcagg cagtagatta cggtattgga tgcggacata cgaagatcgc ttatattggc
    14041 ggaaagaaaa attttcgttc gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa
    14101 aaagcgagca tccctgtcaa tccagcctat tttttaacag gagattacag cttagaaagc
    14161 ggcctccaaa acatggaata cctattatca ctttctgacc tgcctactct ggttttttgc
    14221 ggcaacgacg acatagcgat tggtgcattg agagctgtac atttagctgg cctccgtgtt
    14281 ccccagaaca tttctctgat cgggttcgat gattctccag tcgtctccta tctcaatccg
    14341 ccgcttacca ccgtacgtaa gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta
    14401 ctagatttga tcgaaggcag gacgatcgaa caatctaaaa ttgagctctc caccttgttg
    14461 atccttcgtg agtcgatcaa ttacttgtaa actacaagta attttatata aaaatgttaa
    14521 cgtgtgcata acacagaaag gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa
    14581 aaaacgaagc acctgtaaaa gttcttcagt ttggtgaagg caattttatg cgtggattta
    14641 ttgattggca gattcagcag ctgaataacc aacaattgtt taaaggtaat gttgcaatca
    14701 ttcaaccttt agaacaaggt cttggaaata tgatgaacca gcaagataat ttatatacgg
    14761 tcattttaca aggactcagc aatggcgaaa tcgttgatac ttccgagatt atcaccgtag
    14821 tagaacaaat gatcaatcct tatgatgaat gggatgctta tctatctcta gcagaaaatg
    14881 acgacttggc ctatatcgtc tccaatacaa cagaggcagg aatccaattc gtggagactg
    14941 ataatttaac agatactccc ccacagagct tcccgggcaa actgacagcc tttttatacc
    15001 gacgtttcaa attaaacaaa gctggcttta ccatcattcc ttgcgaactg attgacagaa
    15061 acggcgaacg tctaaaagaa atcattttac gctacgcaga aaaatgggat ttagaacctg
    15121 cttttgttga gtggttagaa aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg
    15181 tcccaggtta tcctcgcgaa gatgcaggtg ttttcaatca acggcataac tatgaagatc
    15241 aattgatggt caaagcagaa ccattcatgc tttgggtgat cgaagggcct aaagaattgg
    15301 agaatcaact accattcaaa caggctggct tgaatgtcat catcacagat gatatgactc
    15361 cttatcgtga acgtaaggtg catttgctta atggaccgca tacagcgatg gtccctcttg
    15421 ctttgttggc ggaattggaa actgtagaag aagtaatgaa agatccttta ttctccgcat
    15481 atgttgatca gttattcaat ctagaattga ttcctatgct ttctttacct aaagatgaat
    15541 tagccattta tgctgaccaa atcaaagaac gtttcttgaa tccttttgcc catcacaaat
    15601 tagaagcgat ttcattaaat agtgtttcaa agttcagcac ccgtctgctt ccagtgttca
    15661 agaagtatat cgaagaacaa aatcaggtac caccgttaat cactgtttca ctagctgcgc
    15721 tacttcttat gtatcgaggc gatcaagtaa agccacatga tgatgaaaag acgataagcg
    15781 aatttactga tgcttggtct gataatggaa cagctattcc tcgattattg caaaacgctg
    15841 cgctttgggg agaagatttg tctcaaatac cgaatgtgac agatacagtt caagaaatag
    15901 ctgcaaagat cgatcaaaaa ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg
    15961 tccatgcttg aaaccctgca accaacaatg aaattaaatc gacgagacag tgtggtcgtc
    16021 gcattaaccc ctattccaaa acaaacacaa ctgacgattg atcagcaaac gatcaccact
    16081 cttgaagata tcccgcaagg tcataagatt gcattgggtg atttgaaagc tggagacaac
    16141 gtgatcaaat atggctatcc aatcggtcat gtgacgacag acgtaactgc cggtcaatgg
    16201 ctgcacactc ataatgtaaa aaccaatctt tctggtgaat tggattatcg atatgaaaaa
    16261 gatgtccatc caagtcatta cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa
    16321 aatggaaaag tagggattcg taatgatttg tttttagtac ctaccgttgg gtgtgtgaat
    16381 ggcattgctg aactgatcgt caaacagttc aaggaaaaac atccagatct gggacagttt
    16441 gaccatatta cgattttaaa gcatccttat ggctgttcgc agcttggaaa agatcatcaa
    16501 aatactcgag aaattttagc ggacgcagta aatcatccca atgccggagg agttttagta
    16561 tttggattag gttgtgagaa caatacagtt cctgaattca aaaaaatttt aggtgaatat
    16621 gatgaagaac gagtgaaatt tctagtggct caagatgttt acgatgagat cgaacaaggt
    16681 gtggctctat tagaagagct tttaacagcg gcggaaaatg accagcgcat atcagtcccg
    16741 ctctctaagt tgaatgtcgg tttgaaatgc ggcggttctg atggtctatc gggaataact
    16801 gctaatcctt tacttggtgc attttctgac tacttgattg ctcaaggagg cagcacgatc
    16861 ttaacagaag tgccagaaat gtttggcgca gaacaagtat tgatggcacg agcggaaaat
    16921 gaagaagttt ttgactcaat cgttgacttg atcaatgact tcaaacagta tttcttgtct
    16981 tacggggaac cagtttacga aaacccatct cctggtaata aagcaggtgg gatcactaca
    17041 ttagaagata agtctttagg ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg
    17101 ctacaatatg gcgaaaaaat cagaaaacca gggcttagtt tattgcaagc tcccgggaat
    17161 gatcttgtag ctgcctctgc tcttgcttca tccgattgcc agttggtctt gtttaccaca
    17221 ggaagaggga ctccgtttgg ctcatatgtc ccaacggtca aggtatctac gaatacaact
    17281 ctttttgaac gcaaaggtca ttggatggat tttaatgcag gcgtcttatt agaacaacca
    17341 atggaagtaa tcttagaatc gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa
    17401 acgaaaaatg aacaaaatga cgttcgagaa attgccattt ttaaaaacgg agtaacactt
    17461 tgattttcat aaaacagtat aaaaagaaag aaggaagatt tatgttttta tctgatgatt
    17521 ttttattaaa agacgattgg gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca
    17581 ttattgatta tcactgtcac ttagatccaa aagaaattta cgaaaacaac aatttcagca
    17641 atctgacaga agcttggtta ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg
    17701 gcgtgcctga agaaaaaatc acaggaaatg cttctgattt tgaaaaattt ttagcttggt
    17761 gtcaaaccgt accaaaaatt atcggaaatc cgctttacag ttggtcacat cttgaattga
    17821 agcgtttctt cggtattgac ttgttgataa atgaagaaaa tgcagagaaa atttggacac
    17881 aagcaaatga aattttggca gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga
    17941 atgtagaagt gatttgtaca acagatgatc ccgcagacga cttgcactat catcagttgc
    18001 ttgctcatga agaaccagat ttgaaggttc taccttcatt ccgtccagat aaagcactca
    18061 atattgaaaa agaaggattt aatgcatggg tcacactact ggaagaagca gctgaccaaa
    18121 cgatcaccac ctatcaagaa ctgatagaag tcttagggca gcggatagaa tacttccatc
    18181 aacatggatg tcgtgtttcc gaccacgctt tagacatctt acgctataaa gaagcggatg
    18241 aaacagtatt agaagagatt tttcaaaaag cacgagctaa caaactatta tcttcagatg
    18301 aaatcgatgc ttatcgtaca gaaacactga tacatttgac ccacttctat cattctcata
    18361 attggacgat gcagctgcac atccatgctt atcgtaactg taatacacaa atgttggaaa
    18421 aattagggcc cgataccggc tatgatggaa tgaatgatct ttctttgact attcctctgc
    18481 aaaaattatt gaaccgtgca gaagaaacgg atcagttgcc aaagacgatc ctttattcat
    18541 tgaatccgaa tgactatcct gcgattttag ctttgatggg ctgtttccaa aaagaacaga
    18601 atggaaaact gcagttaggt tctggatggt ggtacaacga tactcgagct ggtatgcgcg
    18661 atcaattgac acaatttgct gatggtagtg ctttggggaa ttttgtcggc atgctgacag
    18721 attcaagaag tttcctgtcc tacactagac atgaatattt ccgtcgtgtt ctttgtgaat
    18781 ttttaggcga aatggtcatg cgaggagaag cacctgaaga tgaacaactt ttaggaagtc
    18841 tagttcaagc gattagttat acaaatgcta aaaattactt tggctttttt gcctaattga
    18901 ttaatagtaa cagtaacgaa tcaaaaacag gatagtgaaa taacggaatc cgtttatcca
    18961 cgttttagag aagaagacgg accaaatttg ctccgtcttc ttctcttgct gttctgcttc
    19021 attgcctata aacctttatg aaagaagaga taacatgcct actatccata ttgccggtga
    19081 ttctactgcg gcagaaaaag atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat
    19141 ctctgcttat ttttcacccg agatccagat caataaccaa gcaaagaatg gacgcagcac
    19201 caaatcattt atccaggaag gaaggttagc ccgtctagct tcacagtttc agccgaatga
    19261 tttcttattt gtacaatttg gacataacga ccagaaaatc caagaagaaa gaggtacgct
    19321 tcccttcggt agctatttaa aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa
    19381 cgtccaaatg gtcttactga catctgtgac gcgacgagat tacttagaaa acggaacatt
    19441 gaatccagat atacttggag actatccaaa agcgatgcgt gcatttgctg agaaacacca
    19501 tattcctctt ttagatgtgt tctcaagatc acaagaattg ttccagacct tctcaaaaga
    19561 agagacaaaa aaattctacc tgcatcttat acctgatact tgtaaaaatt acccagaagg
    19621 actaaaagac aatacccact ttagtccaga aggcgctgat aaagttgccc gaataattgt
    19681 tgaattgata aaagaatccc gtctgcctct tgctaattat ttacaaaaag gagtttgaga
    19741 tgtttccatt aaatttaggc atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa
    19801 tcgttcaaaa agtatcacag cttcacttgt cccatatcca atttgcacca caaaaagcat
    19861 ttcctgaaca acttaaatta actgacatga cacttgggag tgctgcctat tttggtgaat
    19921 acttccaaaa aaacaatatc gaactttcca ttttaggatg ctatatcaac ctatccagca
    19981 aaaatcaaga agttcgtgca caagcattgg caactttcaa acagcaaatt gctctgtgct
    20041 ctgcctatca agccaagatg attgctacgg aaaccggtag tgtctctgtc ggttacacaa
    20101 aagaaaattt tactgaagaa gcttatcaga tcgccagaaa ttctatcatt gaattagtcg
    20161 ctcatgcaga aaattttggc gtaactgttg ctgttgaagc agggatcaat catccaatct
    20221 ataactatca gttagctaaa cgtttaatcg atgaagtagc ttcaccaaat ttaaaaatca
    20281 ttttagattg tgcgaatttg atgcataaag aaaatcatgc tgatcaggaa aaagtgatcc
    20341 gaggtgcctt ggacaattta catggttata ttacagcgct tcatctcaaa gactatatca
    20401 tggaaaatgg aaagatccgc atggtacctg ttggcaaggg ctgtttagat ttccgcccgg
    20461 tactgcatta tatcaaatat gagcgtcctc taatgtacgc cactcttgaa gctactcctg
    20521 agatttatgt tcctgaagca atagaacact tgcagtttat ttaccaagat tgttaaggag
    20581 aagattatgg aggaacgaat acagaaaagt tatcaaaaat atcatgcaaa tgcttttcag
    20641 acattgctgc atctttatca gggaaattat tggcgttttg ccatgtcagg actctttttt
    20701 attatcaaac atcttccatc ttggttaatg ccgatcgcta ttgccaatgt aatcaatgca
    20761 gcttcatcga aagattcttc tcagttgcag gccatttatt tcaacgcttt tttgatggct
    20821 tgtctgattg gtcaaaatat attgacgaat tattttcatg taaaatacca tagcacttcg
    20881 atccgtcaag tagaagctgg actaagagcg acccttatca agaaaatcca agaactatct
    20941 attccttatc aaaaagaatt gcaatctgga cgaatccagt caaagatcat ccgtgatgta
    21001 gaagctatcc aaacgttgtc ctctcaagta tttgtcagcg gattaaatat cgctgtcaat
    21061 ttattcgtag ctttaggtat cacgctttat aaaagtcctg ttgttttcat gtttttctta
    21121 ttgactgttc ctctcgccgc attcgttact ctctttttcc gtaaaaggat cagacagtcc
    21181 aactcagact tcaggcaaga aatggaagag acttctgcaa aagtgatcga agtgatcgaa
    21241 ttagcaccga ttgctcgagc tcatgggtta gaaaaacacg aaacaactcg cttaaataag
    21301 cattttactc aaatctatga aaaaggattc cacttagata tcctgcaatc tgttttcgga
    21361 tcagtcagct gggctagttt tcagatattt cagctgattt gtttgatttt cactgggata
    21421 ttagctttac aagggcgcat ccaacccggc gatgtcgtga tgtatcagac gtatttcacg
    21481 acgatcgtca attccgtttc tggaatgatc acgctgattc ctacgatttc aaaaggcatg
    21541 gaatctatca cttctattgg cgaactgttg attgctggcg atgtagaaac ttatcacggt
    21601 aagaaaaaaa tccaacattt ggatggaaat tttcgttttg aggatgtttc ttttctttat
    21661 ccggactcac tcaaaccgat tatcgatcat ttttctctag aggtgaaagc tggagaaaca
    21721 attgcttttg ttgggccatc tggctctgga aaatctacac tccttaactt attgattgga
    21781 tttatccagc cgacttcagg aaaaattttt ctagatgatc agcctttgga tgaactggat
    21841 atgcgaagtg tgcgaaatta tttagccgtt gttcctcaaa ccactttgtt gttctccgct
    21901 tcgatcaaag agaacatcac ttatggcttg aaaaacgtat caaaagaacg tttagatgaa
    21961 gtcatcgaag ctgcgcaact ttcttcactg attgaccagc ttcctgatgg tctggatacc
    22021 ttagtaggcg aacatggcaa caagctgtct ggtggccaaa agcaacgtat ctccattgca
    22081 cgagcgttga tacgtcagcc aaaaatcatt ttgctagatg aagcaacctc agctttagat
    22141 aaccagtcag agaaaaaaat ccagcaagcc ctgaattacc tgacagaaac acccactact
    22201 tttattgtgg ctcatcggct ttcaacgatc aaagaggcag ataaaatcgt tgtgatcgat
    22261 gaaggaaaaa tcgttgaaat aggtacttac gaaaaactgt tagctcaaaa aggctatttc
    22321 taccgcttga aaaatgaatg acaaaaaata ggagctatga caagactttt gtcgtagctc
    22381 ctatttctga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatagtcat
    22441 caaacatgag aagctgtttt ttgaaattgt tccttgtaac tcagagttaa acggtaggct
    22501 cacgacctct tggtaaatgg tgttcaagag acagcctatc ggcaataaga agcaattttg
    22561 tcatcgctca aggccgaacg tctctttcac accctctgtt gtttcataaa atagttttat
    22621 tcgctgtaaa ggtcgcgaac tggtgtcatg atgatacggt tcaagtcatt gatgattgta
    22681 ctgaagcctt gctctttttg catcaagtca ttgattactt cttctgattg gattttcaca
    22741 gccatttctt gtgctttcat tgcatcttcg tcgctgaact cttgtccact catttgtttt
    22801 tcttgtaatt cgatttgcat cgcttggaag tctttgaaca gtttgtgtgc ttcttcgttt
    22861 gcttgtactt tctcgtacgc ttctttcaac gctttgaatt ctggtaattc acggatctca
    22921 cgttcgattt gattcgcgct atcgtagatg tttgacatgt tatgccctct tttcttttag
    22981 tagttactct ctattgtaac atggggttat tcgttttcaa aggggctact ctccttgata
    23041 tttccgccag aggtctttag ccgcttcacc aattccctga acgccttctt tcaccatatc
    23101 gccgaagttt ttcagccctt cttttgcttg ttcaccatat tgattcaagt tatcttgcca
    23161 atttccagac tctccattag aatttcctgt gtttccaact tgatctgcag ctaccacttg
    23221 tcctcctgtg gcataggcat ctgctacact aaatcctgtt tttgaagtat acggaaggat
    23281 gccttctgcc gctgatttga agactttccc gacaacattt ccacttgtcc cttccaaata
    23341 gtgcagcttg ctggattctt cgtaacccat ccatgtcgaa atcactacgt ctggcgtgta
    23401 accgatcatc cattggtcat ttgcttttgt ggcatcgaaa ttagtttctg tggttcctgt
    23461 tttccctgct atcgtataac ctgcaggatt cgcagcagcc gctgttccat ttgaaaatgt
    23521 accaagaagc atgctggtca tttcatcggc tgtttccttt gatatgacct gttttggttt
    23581 tttgtttgtg ttatcgacga tcaccgctcc agtcgaatca acgattttcg tgataagatg
    23641 cggcgtatac atctgaccat cattggcaaa tgcactgtaa gcacccgcca tgatcaatgg
    23701 agatactcct ttttccagtc caccaagggc taagccataa tacttatcag attttgctaa
    23761 aggcaggccg aattcttctg ctttatcata tcctttttgt aaaccgatct catgcaaaag
    23821 ccatacagta ggcaaattca aactttgtgc aacagcttga tacatcggta cttctccttg
    23881 gtatgtcccg tcaaaattat gcgcatcgta ataactttgc ggttcgtctt tcaagatact
    23941 ggaaggcttg tatccagctt ccagtgctgg tgtatatacg cttaatggtt tgatcgttga
    24001 tcctggtgaa cgacgcatct gtgtagcaaa gctgaatcct ctaaagacat gatctcctct
    24061 tcgtcctact agtgcttgta caccacctgt attcggatct aaagctactg aagcagattg
    24121 gaccatggca ccatctgaag catttgctgg gaataatgca ctgttttgat aagtagcttc
    24181 taattgcttt tgataatcct gatctaaagc tgtatagatt ttatagcctt tattcatgac
    24241 atcttcttca tcaagaccat actcgttaac tgcttcatcg atcactgcat caaaataata
    24301 aggataacga taaccagaat ctgaattatc gtatgtgtca ttaagatacg cgctgatatc
    24361 tgtctggctt tctgcttgtt cttgttcttt tgtgattttt ccgttttctt ccattacact
    24421 tagtactgtg tttttccgat cattcgcatt ttttgggtga tcgatcggat tataaatacc
    24481 aggacctttc agcattcctg ccaaagtagc agcctcagac agcgtaacat tcgttgcatc
    24541 tactccaaaa tactttctgg aagcgtcttg gacaccccat accccgttgc caaaataggc
    24601 attgttaaga tacatggtca agatttcttc tttgctgtac ttcttttcga tctcgattgc
    24661 caaaaataat tccttcgctt ttcgatcaaa cgtttggtct aatgtcagat acgcattttt
    24721 ggcaagctgc tgagtgatcg tacttcctcc gccccctgat gttccgccag acaagaccat
    24781 tcggactgct gcacgggcaa tccctttgat atcaaacccg tgatgctcgt aaaaattcct
    24841 atcttctgtc gagattactg catcttggat atagggagaa atcgcgttta attctacata
    24901 tgttcctttt tgggcgtaca gtgtgccagc ttcttcgttt ttcttatcat aaatgactgt
    24961 cgattggctt aatcctgatt ttaacgtttc gacatttgct tgttttgcta aaataaagag
    25021 gtagatactt gtcactaaga cacagatcaa accaatcagc aaaatcaatt tgttcacatg
    25081 atattttttc cagatgcgct ttcgccatcg atggaattgt ccgagatatg gtctgatcca
    25141 acgccaaaaa ctgatcaaag cagcttttat cttttctaaa atgctttgaa aatccatttg
    25201 cttcttctcc tgtctatttg attcaatcgt cttattttac catagtgaaa gagtgttttc
    25261 ttcctgctta agttgtagtt caatatgttt ttttacgtta gtgcaagtag agtaacataa
    25321 gaatgaacag aaaaatgtaa gttttttctt aaatatgttt taaaaatggt acaattagtc
    25381 tgcgattgca ctgaaaggag tacacttgtg aaaatcacga tcactttacc tatgaaccat
    25441 cctgaaacaa caattcgcga cttgcttgaa aaggaatggt tagttcctag aaaggttcga
    25501 cactttttgc gtatacgtaa aaacgtttgg atcaatcaac aacctgcttt gttccatcat
    25561 gaagtaaagg gcaatgacca gatcacattg ttattcgaag aaacagatta taattatcaa
    25621 gaagttcaat taggagatgc gaagcacatc cgtgttttat atgaagatga gcatttgatt
    25681 atagtggata agcctgcagg aatgaagact catccaaatg aaccgacaga aaacagtacc
    25741 ttactcaatc atttagctgc ttatctcgac actaaacagc aacgacctta cgttgtccac
    25801 cgcttggaca aagaaacaag cggtgccgtt ttatttgcaa aaaatccatt tgtcttgccg
    25861 atacttggaa gaatgttgga acaaaaaatg atctatcgga gatatcaagc catcgtatgg
    25921 ggagaaattg ccaaagatat gaccatcacc aataaaattg gacgagatcg ccatgatcgg
    25981 agaaaaaggg tcgtagatga taaaaatggt cagagcgcct tgacacatgt cactgtcaac
    26041 aaagtacttt ctgaacaaac acaagtctat tgtgttttgg aaaccggacg tacacaccag
    26101 atcagagttc atttagctca taagggacat ccgatcgttg gagaccctct ctaccaaaat
    26161 agaacagctg aacgactgat gctccatgca ttagaactgc acatgatcca tccatttact
    26221 caagaaaaaa tagtcggtaa agctttacct ggattatggg aatagaaata aaaaagaaca
    26281 gagtcttatc agcagataat gataagactc tgttcttttt gtgtaatttg tctgcttact
    26341 tttgatgttc gtgttctgct aacagcaaac taccgataat cccactttca ttcggcagtc
    26401 cccaacgaac gatatattca cttacttgag gtgtttccat ataaccaccc atcaattcaa
    26461 caaattgctg acggattttt tgaaggagat gatcctgatt cattacccca ccgcctaaaa
    26521 taatcttttc aggtgctaac gtcaacgtat agttgatcag tgcttgagca atataataag
    26581 cctgaatagc ccaaacagga tgatcttgcg gcagatcttg ccctttgatc cctgtgcgtg
    26641 cctcaataga cggtcctgct gctaatcctt ctaaacaatc tttatgataa ggacaagtac
    26701 cttcataatg gtcatctgga tggcgtttca cccaaatatg tcccatttca ggatgtgcga
    26761 tccctgaaaa aatttcttca ttgatcacga cacctgcacc gattcctgtt cccacggtaa
    26821 ggtaaataca actatttttt ccttgtgctg ctccttgctt caattcccca tatgcagcag
    26881 cattgacatc cgttgtccaa gcagcaggaa tattgtagcg ttccttgatt gtgccaagaa
    26941 aatcgaattg tgcccaccct tttttgggag ttgccaaaac gtatccgtaa ttatcttttg
    27001 ttttgtcgat accaatcggg ccgaatgagc caatcccgat tcccttcaat tcataacgat
    27061 caaaaaattc aaatacctga tccaatgttt cttctggtgt tgtggtaggg atacttaatt
    27121 tttctaaaat ttctccacca tctgaaaccg cacacacaaa ttttgttccg cctgcttcta
    27181 ttccgccata cattcgtttt cctcctcgaa tccgattgtc cgaatctcat aaggttggat
    27241 tattgggaca accgcttgct tcgttggttc ttcaagcaga ttcaatatga cagtttttcc
    27301 attgtcttta gttatttcca atttttctaa atgactagac atattgaaac ctcttactac
    27361 tactttatca ctaaacttgc tgcgtttcaa agctgtaatt gcaaacgttt cactttttat
    27421 cgttaaatat tgttgttttg aaatcaatgt tccttcatga tgtttgattt gttgagtgga
    27481 aaaaggaatc tgtgctgcgt atgcatgcaa ataagtagag aatttttctt ctggtccatg
    27541 tagttcaatg ctgtaattga accgatgctc acctagacac tgtgcttctg gtgttggaaa
    27601 atatccccaa tcgcctagtt ctcccacact gcgaagtagc gtcactgcga tttgtccgtc
    27661 ttctgttact tcatattcat tcaaaccaaa gtttccgact gttacaccgt actcttcatc
    27721 atgaatattg acaaatgctt gctgatgttg cggattcgtg gggttttccc aacttttaga
    27781 cacatggttt gggcgcttca ccacttcaaa aatactatct gcttcgtgat gttccacatg
    27841 taactttgta ggaaacaaga cacgtaagcg atgatctttc atctgattgt cgagagtcgt
    27901 ttcaaagtcg atttttttac tatcttttct gattgtcatg attgttttta tttgaaggat
    27961 tcgtttttct ttggatcgtt ctgcttttcg ataacgaaat tccacaacca tctgctgttc
    28021 tttttctaaa cgctcgtctg cagaaacagg gatttccatt tcctgtagaa tacttatttt
    28081 tgctatttcc ggtgtatcta ctaatacttt tacttcagag ttttctttgt tactggatag
    28141 gattggctga tcacagaaag gttgtttaaa gatatactca tttcctatat cgccgatatc
    28201 ttcaaaaata tgcagattat tcaactttcg tttgtttttg cgatcttcca ttgtaatggt
    28261 accatttttt tcaatcgtca aatgtagagg gccgttttcg atgattcgtc cactctgatg
    28321 aatcattgtt tccttttctt ctgctttcgc ttccccttct acaagcgcga atgtttccca
    28381 agaaaatgct ggcatgtttt ctaatggtaa agtcactttg atcattcttg ccatatatgg
    28441 cacacggaaa cggtctttcg gcaaatcata accaaattgg acagtttctt cagataaaat
    28501 agctggtata gtggccccac tttttgtttc tacatgatac ttacgtttag gcaaattttc
    28561 taactcttga tatagttgtt caggtattcc ttcagcgaat ctttttcttt ctaattcgat
    28621 ggttatttct gcttcaccag ttttatccat tcctgcagta tttacaatga caaatggaaa
    28681 gctgtctttt gggaatccgg tcgtatcgat tgctgcggtc agctgttcta aggaatcttg
    28741 tgcaagatac tttccaactt catctgcttt ttcaaaacga ggtatcattt cccgatggac
    28801 agaatcgact gaacaaccgc aaatactgtc gtgaggatga ttttgcatta atgtcttcca
    28861 agcataatct aattgatcat ggggatagtt tccactaact tcataagcca tcgtcgcaag
    28921 tggttctgta atattttcta gctgtcgctg gaccttggta ttccattgtt ttaagtaaac
    28981 ccgagcagat gctgtattgg ctaaagtata ccagccatcc gtttcttgac ttgtcaattc
    29041 tccttctact gagcctaagt cttctggcac atctttttga actgcttcca gatagtcagt
    29101 aaaattagaa tgaataaact cgtaatctgg aaacagttca tttgcgagat ggattgcttt
    29161 tgatatatct ttttgtaccg gttgatgatc aacaccattc atcatcaata agtggttcgt
    29221 agaggcatat tgttctgcat ccgctaattt ttgcttccaa aaggcaagag ctgcttcttt
    29281 ttctactgga atctcattcc cgttgctata ccaattggca aatagcagac caaagattgc
    29341 tgtttgatca ggtcctttcc accacatctc tgagtattgg gaagaatagt tttctgcttc
    29401 caaaacttga ttatcaaagc caattggttt tacaccacgg ccaaacgctg ctgctgaaat
    29461 accagcttgt ttcattaatt gaggcgtctg tcccatattt ccaaatgtat cgggaaaata
    29521 gcctaacatg acaggggttc cccatttacg gctttcttcc atgccaatca gcatatttct
    29581 cacgttcgat tccgagctga taagaaaatc atcttgcaag atataaaatg gtccaattct
    29641 caattttcct tcattgattg cttgttggat tgcctgtctt ttttctggcc ttacttgaag
    29701 atagtcatct aaaataatcg tttgcccatc caaatgaaaa ctgttgaagg acggatcaac
    29761 ttcaaatagc tctagtagat cgtctatcaa ttcaatcagt cgcatgtgat gctgttcgta
    29821 agccatgtac cattcacgat cccagtggct atgagaaatg atatatactt tctttttcat
    29881 ttacgatacc tcctactttt ctactctaat atcaaagtaa tccatgacta attcgcaaaa
    29941 catcatattt gcccaagaaa accattctct cgtataattt tcaggattgt tcacatcgaa
    30001 tccttcgtgc ataaggtgtg ttcctgcgtc agttgctaca agtaaattca atatccgctc
    30061 tttttcagat ttgtctgacg ttgtcattcc ttccatagct aatgcaatcg gccaaacgta
    30121 tttttctggc gtatgggagg atccaattcc ttttgcgtac tttccttcat agaaatatgg
    30181 attttcttta ctcaacaatg ttcttcttgt ttgcagataa tcttcctctt gttcagaaca
    30241 atatcccaag taaggtgcgg cgatcagatt gggcacatta ctgtcatcca tgactgtggc
    30301 gtttcccatt ccatctactt catatgcata aatcgtttgc cctttttgat taaccgttcg
    30361 tccgtgattc tgaatcccct cttggatctc tttcttcaat ttctgtgttt gctgtacaat
    30421 ttctttatca tcgagtatcg tttgaaaaat ttcttccaca taacctaaca cgaccaccgc
    30481 aaacatattg gatggtacca gatatccata acggcaagta tcatcgctag gacggaaacc
    30541 agaccatgtc attcctgtaa aagctgtttt tgcacctcgc ccgtcattga tcagcgtatc
    30601 ttcttttctt gtcgtgtctc gtacaaatgt ataaggagag tgttcatgat cttgctctgt
    30661 tttgaacacg tccaagattt ttttgatacc ttcaataaaa gattgattga attgatcagt
    30721 ttttcctgta tttttgtaaa gaagataggc caactgtatt gggtagcata aagaatcaat
    30781 ttcatatttt ctttcccaga tccaatcgtt catttttgta tgatctgttt ggtggccagc
    30841 accgttagct tcttcgttga aggcattcgc atatggatcg atattgatat aaaaaaattg
    30901 ctttttgact aacccgctga tcatgtcagc taattcctca tcttcttttg caatcactaa
    30961 ataaggacga acttgtgcag tagagtctct taaccacatc gctggaatgt ctcccgtcaa
    31021 taaaaaggtg gttccgtcat tttgcttttt gactgttgtc aacagagtat tggcaaatgc
    31081 tgctgtaaaa ttctttgccc agtccgcatg gatatcccca catttttgtg tgatatcttc
    31141 cataaatttc tgcaccgatt ttggtatatc tttataagcc actaataaaa ccttctttct
    31201 atttgatata tctttaaaga ttatacttca tacagccaat tatgtctacg ctttcaaaaa
    31261 atatcttttt atttactttt tcatccatta tggtattatt tgaatatgat atatcattaa
    31321 gaggtgttta tatgaatcaa ccattgtacc agacaatatt taatgatttg caaacggcta
    31381 ttttaactgg tgaattacct gtagacagcc aactaccaac tgaaaaagaa ctttcacgta
    31441 cctataaagt aagcagaatc acttccaaac gtgcattgac tgaattagag caattaggac
    31501 ttatttatcg cgttcgcggg aaaggcagtt tcgttaaatc cgcttctccg gaacagattc
    31561 ttgcttctcc tacaaaaaca cgacggattc tgtttcttct tccatatgtc gctgatttgt
    31621 ctgttggtga ttttagcaag ggattgaacc ctgtgatgca agataaacag attgatgtca
    31681 tgatgacgac gcttgacttt ctacaaaata aaacagcaaa agagctaatc aatgaatttg
    31741 acggattgat ttattatgct cttgatacag aacaacatct tgacttactt tttgagcttt
    31801 cattgaaaga attccctgtc gtcatattag ataaaaaaat ctatgagtta ccttttccta
    31861 ccgttctttc cgataatttc caaggcggtg cgcttgctgc ctcttatttg atcgaacagg
    31921 gacattcaaa aatcgcttat ttgtttggta cgcagactca tccacaatcc gttcgacagc
    31981 gttaccttgg ttaccttgaa gcattggaca aagctggttt gactttccac acttcactgg
    32041 atgacaaaag cgcgacatta gatgaattgc tttcttatat cactaagtat gaagtaactg
    32101 cttttgtttg tgaaaacgat gttgtcgcta tccagaccat gaatctcttg agacagcatg
    32161 gttatcatat ccctgaagat ttttcaatta tcggatttga tgatatccaa gcagcagctc
    32221 ttgttgaccc gccactgaca accatcgcac aagattttga acagttaggt aagcttgcgg
    32281 gagatgcttt ggtcacttgg ttagagacaa aagatcgacc atccgatagt aaatttccag
    32341 tttcattcat caaaagacaa tcaacaaagg agaaataaaa tgaatatcgg tcaaatagat
    32401 actagacatg caacagcgaa tcagccaaat ttctctaatg ggaactgcca gccttatact
    32461 ggcgttcctt ttgggatgaa ttattttgca ccgcaaacta cggatcaaaa cggcagctgg
    32521 tggtttcatc ctgatgaccg cgtttttcaa ggctaccgtc ttacccatca accaagtcct
    32581 tggatgggtg atttcagtca tatgctgttt acgccgatca atggaaaact acaggagaac
    32641 acccttttcc atgctcaaag ttcttaccgc ccagaagaaa gtatattttg cccgactcat
    32701 ctgtcgatct gtcaattacg ttatggcatc tgttctacat tgatacctag catgtatggc
    32761 ggaatcttat ctattgatta cagcaaaaat gacagcggtt tacttttatc ctttccggga
    32821 cgccatcagc tctttgttat agatccttac acagtggaag gaaaaatcac acattttgct
    32881 ggttcagaag atcctgattt ttctttttat tttatattga gatttgaata tcctttaaca
    32941 attgatgagt tagctgtttc caacaaaaat agcgactcga tcccgttgta ttttgaacag
    33001 acaaaaaaac aaactatccg atttgggaca tcttttatca gctcggaaca agcagcttat
    33061 aacctggaac tagaacaaaa ccaaagcaaa gagacttacc ttgctcacag tcaagaacaa
    33121 tggcagtcct attttgatcg tatacagctc acacaccatg atcaacaaca attgcggacg
    33181 ttttatcata atttatatcg ggtgttttta tttccacaaa cattttatga aattgatcct
    33241 agtgggcaaa aaatccacta tgatacgact agtcgatcta taaaacctgg tgttctctat
    33301 acaaataatg ggttttggga tacgtataaa acggtttatc ctctttattc tttgattgct
    33361 gtagaaaaat acgaagaaat gctggaaggt tttttgaaca gctatagaga aagtggctat
    33421 ttgccaaaat ggctgtctcc tgatgaacgt ggacttatgc ctgggacatt gatcgatgcg
    33481 gtcattgcag atgctgctgt aaaagatatc cgacgagact tgatgcctga atttctaacg
    33541 gcaatggaaa aaggtgctac ggttcaaagc aaacatccaa attatggacg ccaaggaaca
    33601 gaagattact tgacatatgg ctatgttcct aatcactacc acgaatcagt caatcacaca
    33661 ttagactatg cttatagcga tttttgtatt agccaagttg ccaaggtact agaagaagag
    33721 gaaatgacga agaaatatca gcagcaatca ttgaactatc aaaatatctt tgactatgag
    33781 tcaggattta tgcgttcaaa agataaagat ggcaagttca gagaaccatt ttcagctaat
    33841 cgttggggac aagattatgc agaaggaagt gcttggcaaa gcagctttgc tgtttatcac
    33901 gatttttcag gactgatcgg aaaatatggt ggcagtgaga gatataaaga aaaactcatt
    33961 gagctgtgca atcagccccc aacgtttgac ccaggaggat atggttttga gatccacgaa
    34021 atgagtgaga tggccgcgat agaatttgga caattagcta tttcaaacca gccaagtttt
    34081 cactatcctt acttgttcag ttatatagga caacctgaaa tggcacagcc actgcttaaa
    34141 cagttgatga ctcagacctt cgacgattct ccaatgggtt accctggaga tgaggacaat
    34201 ggaagtatgg ccgcttggta tatcttcaat agtctgggat tttatcctgt cacgcctgga
    34261 accggtgaat atgttatcgg tatgccatta attgacaagg ctgttttgct tttgtcaaat
    34321 ggaaaggaat tgacgattga agcaacgcct aatcggccgc agcaacaatt tatccataaa
    34381 atcaaacgga tggacaaaga atatacgaaa ttatatttta cccatcaaga tttgctgaaa
    34441 ggcggcacga tctcttacca attagggatc atccctcacc ctaagaaata tcaagcaaaa
    34501 gatttgcctt tttccttatc aaactataat taacaagagg ttgtgaaaaa gctgtcctgc
    34561 atcaagtaat aagaacagcc aagcaaaaat agatcctcat atttttcgtt tggttggtct
    34621 tattaccgca gatgcaagct tttgaacacc gtttattgag aggttgtgaa atcagcgttc
    34681 tgacctgagt attagagaga caaatggcaa aatagttcct catattttgc cattttttga
    34741 gctaataccg agggtcagct gattgaacac cgtttagtgg agaggtcgtg aaaaagctgt
    34801 ttagtttcga gtattaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
    34861 tggcttaata ccgagaaact ggcttttgaa ctccgtttat cgagaggtca tgaaaaagct
    34921 gccttgcctc aagtaataag aacagcccaa aattagattt ctagacaatt ttttataagt
    34981 tatttcatta cattggcttt ttcttttatg attctccact gttttctcgg tactctttag
    35041 gggatctgcc gacgattttc ttgaataatt tagagaaata cgtggtgttg ttgtagccaa
    35101 tctcacttgc tatctcattg atgttttgat tactgtgcaa aagcagttgt ttcgcttttt
    35161 ccatacgtaa atggttcaaa taagctgaaa aacttttttt cgtttctttt ttgaacagct
    35221 gtcctagata catgacattt aaatgcaggt tttctgacac ttctttgagt gtaagtggtt
    35281 cttgataatg ctggtgcagg attacgatta ctttttccac attgtcactg tactttttct
    35341 gtctctgatg ctttttgatg aatgacagaa gtagctgctg aagttcttgc accgtttttg
    35401 cctgatggat tttttcgatt ccttgtaaat attcctcatc ttccagatca atcaattccc
    35461 gttgaatctc cataaacagt aaaaaactgg aatatcgtat atcttccggc atcatggctg
    35521 ctaactgaaa ctgttcaaaa aaatcagtga tcattttttg agcgacttcc aattgtttat
    35581 tttgcagcac gcgtctgaaa cttgaaaaat cgatggtttg atcttttgtt tctgcttgta
    35641 ctgcgtaaaa aacatgctcg tctttatctc cataaaattg atgcaactgt agattgtctt
    35701 tcgcttgttg gaatgtttca gggattttgt ccaaagaatc tgtctcttcc ccgatactga
    35761 tcagccattc ctgatcagga aagttttctt gaacaaaaca acagaagcca tcaacttcat
    35821 ctgcctccaa caacaacgtg atcaatacac ggtctccgta atttcgttga tagtaaaaga
    35881 tttgttgccg ttcttgcaac catctattga taagagtatc atgagttctt gccagttgaa
    35941 tcaatactat cctatgctgc ttctcactta tttgtcctat tatttcttct tcacttattt
    36001 catccaattc ttcattcaac cattgactga ataacagttc ttgatagatt tcttgttgat
    36061 ttttttgttc attttgctga tctaaccgtg ttttgatttt cttgaggctt tcaaccaatt
    36121 cgaacttatt cacgggtttc atcaaataat tcacggcgcc taattgcata cctcctttca
    36181 aataatcgaa ttcttggtat cctgataaaa tcataaattc aaaattatga cgttcttttt
    36241 gagccgcttc aataaattcc agtccattta tttctggcat tgtcacatcc gttactacta
    36301 aatcaatctg tcgctgttca agtacagcta gtccttcttt ggcattctct gctgttgcta
    36361 ctatttgaaa gcctaaactt tgccaatcaa ttatcttttt taacccattc aaaatcatgt
    36421 attcatcatc taccaataac acttgataca cgctcatccc tccttgaatg taatagaaac
    36481 ggtcagtcca cctgtctcat tattctcaag cgacatataa taactgctgc cgaagctggc
    36541 acgtaaacgt tcattcacgt tttgcagacc gatagaaccg tgaagttcca ctttaggatg
    36601 agacagtttt tcttcaatct gttttagccg tttttcagtc atcccttttc cattatcttt
    36661 gatgatgatc cttacccgtt tgccttcctg caagactttg acacttagcg cattatcgaa
    36721 tcgagtaaaa tcaataccgt gtttaaaata attttcaact aatggctgga tcacaaattt
    36781 aggtacttcg atcttttcca aatcaggatc gatcataaaa tgataggcta ctcgattagg
    36841 ataacgcatt tgatacaaat agacatattt ttcgcaaaag tccagttctt cttttaaagt
    36901 gatcgttttc tcttgattcg tattgtttcg aagcaaagcg gaaaacgcat acacaacatc
    36961 tgctaattct tcacttcctt cgctgattgc atacattcgg atatattcta atgtattata
    37021 taaaaagtgt ggattgatct gtgcttgaag cgcccgcata tgtgcgtctt gctgtttgat
    37081 ttctaatttg tagatgtctt ctacaaactg attgatgcta tccaacattt cgttgatacc
    37141 gattgaaagt tccttcagtt catattccgt gtttgttatg tcgatccgcg tctctaaatt
    37201 ccctgctgct gtttgatcca tggcctgcat gacgatagat acttgctgtg aatatcgttt
    37261 gaatgtttta taaagaaaga taagcagtat attgtctaaa ataatgatac caatgatcag
    37321 cggggcaaga ttttttcccg tttcgatttt tacacttttt ttatctagta cagctaatat
    37381 acgataatca tcagacagtt cttggtgttg aacaaaattg tttttttcca gatcgtcaat
    37441 cggcaattgt gaatttttct tcagactctc tgtgatgatc atttcttgtc tttcaaacgt
    37501 ttctttagct attttattat catggaatgt gtatagtcga tttgtcgttc cagaaatcat
    37561 gtataaagag agtccattga atgacgttag attttctaaa atcatatcta gttctttctt
    37621 ttgaaaacca ataaaaatac ttcctgactc agctcctcct tgtgtgatag gcaacttgat
    37681 ataaaaggcg tctagtaatt ttggtgtccc cttttttatt tccccacctt tgttttctct
    37741 agaagattca taatactccg gtagttgatt caacacgata tatatcgcac ttagttcttc
    37801 ataatttgcg tagagatctt tgatttgatt tgggaaagaa actaattcat cggtactgtt
    37861 ttgctgttcg tatacataca tcagatactg atctatgggt tttgttaagt aggtcgtcac
    37921 attttcgatc ttttcggaat tccctacaag ctggtttttg atgatcctac tcaaggttgt
    37981 tttatcattc agcattctgc ttgtgctttg aacggcatca cttgtcatgt tttctgcctg
    38041 tttttgattt tgttcccaag tatggatact aaaaataacg gtagcagtca tcgttacacc
    38101 gatcatgata accgcgtatt tccgcaacag acgatttagt aaggttcctt ttgaaaatag
    38161 aatccgaaac ttattcataa ctttccaact gtttatctaa ttcttcacgc caatgtgttg
    38221 tcatcgtacc attccaaacc gtcaaaagac caatgacacc aaacaggatc aatcctttga
    38281 attgccaagt cacgcctaaa attataccgc taccaataat tatttttaga aaagacgaaa
    38341 agctggaaaa aaccgaaata aaacttagct ttagcagatt aggaacagag atcgtatatt
    38401 cactatctaa aatcatactg tattgataag tagcaaatag gcagagcaag gcaaaaagta
    38461 aaataaaatc aatagctaaa aaaatgagcc ctttgatttg aacagataaa tataaattgt
    38521 aactcaataa agcagtacca gataaaaata aactaaatag cagattgcct cttaaaaaat
    38581 ttcttttata tatattccag cctcttttta ttgtgatttc tttatactcg aatccgtaca
    38641 aataaaatag ttcattgact gttttccatg ccgcacctaa tccaaagatg atcccgccgg
    38701 caaatgtata tatccagaaa tacaaggtta gttttataat gacccagatc cggataaata
    38761 actcttccaa aaccttccct aacatatctt ctcctccaaa cattaagttt atactttctc
    38821 attatacaaa aaaaggttgg gcaatcagca ccttgacctg attattagag aaacaaacgg
    38881 aaaaatagtt cctcatattt tgtcgttttg tgagcgaata ccgagggtta gctgattgaa
    38941 caccgtttac caagagtttg tgaaatagcc gcttagtttc gagtagtaag aaaaattttg
    39001 tgaatatggc tttccacatt tttacatata tttagcttaa taccgagaaa ctggcttttg
    39061 aacaccgttt attcggaaca agaggttgtg acacgtcttt tgtcacaacc cctttgtctt
    39121 tattcattat tattcttgtc ggaactcatc atattgtttt tgcatttctt tcaatacctt
    39181 atcatagcca gccttgtcca aagcgtcttt caatttcggt aatgtttcat ctggatcaac
    39241 agtccctgta ttcaatccat ctaaatattg attcatgaca ttagaaatat tacttaattc
    39301 tgttttgaca ctgtcagtgt tgaagctgaa tcctaaaatc ggtgaagttt ctgcatcagc
    39361 aatcgattga tcacgtttgg caatcatgtc atccgtaata gactcttgtg tatatagaat
    39421 cttgttgttt cctgtattcc aagcagacat atgtgtattt ggctgataac catctagcaa
    39481 tttgatttta tcatctgaac catcgacttt ttcccatgct tctccctcga tcccccaaac
    39541 aagcccattc aataattctg gatcactgtt aagcagccct agaagttcta ctgatttttc
    39601 tttatttttt gaagtatttg atacgacaaa atttgccatt tgtgcttgag ctgtagattt
    39661 tagtggaaca gtaattgctt ttgagacaag ttcttgtttt gctgcatttg tcaaaattgt
    39721 atcgccataa tcatacggtc cttgtgtttc tccacgcatt agccaagtat ttccttctag
    39781 tggataatcc ttattgctcg ttgcagcgtc acttggaatg agtccttgtt tgtaccagct
    39841 atgcattgta cgtaacagtc cgatatacgt ttcattatcg tattggttga tgattttcga
    39901 atcatctcca tccagatcaa tagcaaatgg taacgtattc cctaaaggat aatcaaagtt
    39961 cccttggact ttgaagcctt gtccaatggc aaaagctgcc gtgtttggtt ctttttcatg
    40021 gaattccttc aagacttttt ctgcatcagc gtaagattgg attccatcaa ttgaaagatc
    40081 gtatttatct agtaaagctt tgttaaaagt aagtacttgt tgagcaaaga catttccatt
    40141 gactgggaaa gcatataatt ttccattgac cagatttcct ttaatatatg cttcatctaa
    40201 atcattataa gctttttcag cgtacttagg cgctaattca gtcaaatcag caaaggctcc
    40261 tttttgggca ttggaaacat agttgttcgc aaaggcgata tcataatttt ccccggaaga
    40321 aatgatgacg ttcattttct tttcgtaatc gccccagcca atgtaattga tattgacttt
    40381 tacaccgatc tctttttcga ttcgtttgtt tgctacttcc atcaattgat cgaaattatc
    40441 tggtttatct ccgatttgat acatctgcaa agtgtccgaa ttgctagctt ctgcctggtc
    40501 tttagaacca gaacctccgc atgctgctaa tgttcccaac aacaacgctg aaacacctgt
    40561 aaaggctagt ttcttccata acttcatttt tcttcctcct aattttatga tttatatttt
    40621 tgtttcttta ttcttttact ccgccgattg ttagaccttt gacaaaatgc ttctggaaaa
    40681 atggatagct gatcgcaata ggcaacgtcg agatgactac gatcgccatt ctggcagatt
    40741 ctcctggaac tgaggctaat ccgcctgtca attggcttcc tgctcctgaa ttttgtgtca
    40801 gatattgaat attactttgg attttcatca aaagatactg tagtggaact agcttatcat
    40861 cttggatgta tagtaaagca ttgaaccagt cattccagta acccaatgcg gcaaataaac
    40921 tgattgtcgc aatacctgga acagctaacg gtaaaacgat ctgaacaaat attcgcagtt
    40981 cgctagctcc atcgatccgt gccgattcaa taatcgaatc agagaccgaa cgtttaaaga
    41041 atgtgcgcat cacaataata ttgaagggac tgacagccat tggcaagatc aatgcccaaa
    41101 tcgtatcttt taactgaaga agatttgtca taacaagata attggccacc atccctggtg
    41161 agaacaacat cgtaatcaaa caaaaaacgg taaaaaaacg acggaatgga aagtttgacc
    41221 gtgaaatcgc ataagcatat aaagaagtag ccgtgctatt gatcagtgta cctaaaacag
    41281 taacaagtac agtgacgaaa agcgcttgga aaattttatc ctgcatctgg tcgaacaagt
    41341 acgtataacc aaatatactg aacttttctg gccaaaaact gtacccgtga gtcgacaata
    41401 tggactcatc ggtaaaagag atggcgataa caaatataaa tggcaaaata caggaaatag
    41461 caaagagtgc aatcattata ttagagaaaa aattagccgt cttattgaaa gagcggactt
    41521 ggactctctc cactttcttt tttttcattt ttttcctcct tagaataatg cagaatcgct
    41581 atcaaaacgt cgggcgatcg tatttgtgat catcaacaag acaaagccga caactgattg
    41641 gtaaagtcct gcagcagccg tcataccaaa atctcctgtg gatgtcaggc cattgtagat
    41701 atacgtatcc aaaacttgag tcacatcata taatgcaccg gaattcctag gaatgttata
    41761 aaacaggcca aaatcagctc ggaaaatgtt tcctacggct aaaatcgtta aaatcgtcat
    41821 tagtggaata agttgtggta tcgtcacatg tttgatctgc tgccatttgc tagctccatc
    41881 aaccatagca gcttcataat aagtcggatc gattcccata acagatgcaa agtaaataat
    41941 gctgttataa ccaatccctt tccatgtacc aatgaacacc aagataaacg gccaatattt
    42001 cggttcatta taccaattga cagcttctcc tccatgtcca gtgatccact gattgaatat
    42061 tcctttgtca ggacttaaaa aagcgtaaac aaagtaactg ataatgaccc aagatagaaa
    42121 ataaggtagt aaggacatcg tttgataaac tttaacgagt cgtttatttc tcaactcgct
    42181 cataatgatt gcaaatacga ctgagatcag cagattcaaa aggataaatg ctacattata
    42241 taaaattgta tttttagtga tgagaaatgc ttgatccgaa gaaaataaaa atttgaaatt
    42301 ttcccaacc
//
LOCUS       DANKMN010000015        41515 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000015 DANKMN010000000
VERSION     DANKMN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..756)
                     /locus_tag="PW224_000815"
     CDS             complement(<1..756)
                     /locus_tag="PW224_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2907523.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
     gene            complement(1014..2291)
                     /locus_tag="PW224_000816"
     CDS             complement(1014..2291)
                     /locus_tag="PW224_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907524.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(2370..3014)
                     /gene="pcp"
                     /locus_tag="PW224_000817"
     CDS             complement(2370..3014)
                     /gene="pcp"
                     /locus_tag="PW224_000817"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL2907525.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(3213..4157)
                     /locus_tag="PW224_000818"
     CDS             complement(3213..4157)
                     /locus_tag="PW224_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL2907526.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(4159..4845)
                     /locus_tag="PW224_000819"
     CDS             complement(4159..4845)
                     /locus_tag="PW224_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL2907527.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            5319..5639
                     /locus_tag="PW224_000820"
     CDS             5319..5639
                     /locus_tag="PW224_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL2907528.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            5793..6698
                     /locus_tag="PW224_000821"
     CDS             5793..6698
                     /locus_tag="PW224_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2907529.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            6695..7468
                     /locus_tag="PW224_000822"
     CDS             6695..7468
                     /locus_tag="PW224_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2907530.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7649..9073)
                     /locus_tag="PW224_000823"
     CDS             complement(7649..9073)
                     /locus_tag="PW224_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2907531.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            9284..9730
                     /gene="nrdI"
                     /locus_tag="PW224_000824"
     CDS             9284..9730
                     /gene="nrdI"
                     /locus_tag="PW224_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2907532.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(9795..11777)
                     /locus_tag="PW224_000825"
     CDS             complement(9795..11777)
                     /locus_tag="PW224_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2907533.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11791..12687)
                     /gene="murQ"
                     /locus_tag="PW224_000826"
     CDS             complement(11791..12687)
                     /gene="murQ"
                     /locus_tag="PW224_000826"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2907534.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(12690..13751)
                     /locus_tag="PW224_000827"
     CDS             complement(12690..13751)
                     /locus_tag="PW224_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2907535.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(13962..14528)
                     /locus_tag="PW224_000828"
     CDS             complement(13962..14528)
                     /locus_tag="PW224_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2907536.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(14556..16058)
                     /gene="eat(A)"
                     /locus_tag="PW224_000829"
     CDS             complement(14556..16058)
                     /gene="eat(A)"
                     /locus_tag="PW224_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2907537.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(16316..17437)
                     /locus_tag="PW224_000830"
     CDS             complement(16316..17437)
                     /locus_tag="PW224_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2907538.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            17563..18504
                     /locus_tag="PW224_000831"
     CDS             17563..18504
                     /locus_tag="PW224_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2907539.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(18654..19550)
                     /locus_tag="PW224_000832"
     CDS             complement(18654..19550)
                     /locus_tag="PW224_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2907540.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            19819..21159
                     /locus_tag="PW224_000833"
     CDS             19819..21159
                     /locus_tag="PW224_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2907541.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            21377..21820
                     /locus_tag="PW224_000834"
     CDS             21377..21820
                     /locus_tag="PW224_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2907542.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(22324..23742)
                     /gene="nhaC"
                     /locus_tag="PW224_000835"
     CDS             complement(22324..23742)
                     /gene="nhaC"
                     /locus_tag="PW224_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2907543.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(24145..25368)
                     /locus_tag="PW224_000836"
     CDS             complement(24145..25368)
                     /locus_tag="PW224_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL2907544.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(25399..26238)
                     /locus_tag="PW224_000837"
     CDS             complement(25399..26238)
                     /locus_tag="PW224_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2907545.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(26255..26935)
                     /locus_tag="PW224_000838"
     CDS             complement(26255..26935)
                     /locus_tag="PW224_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907546.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(26937..28010)
                     /locus_tag="PW224_000839"
     CDS             complement(26937..28010)
                     /locus_tag="PW224_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2907547.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(28066..28304)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(28347..28580)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28657..29328)
                     /locus_tag="PW224_000840"
     CDS             complement(28657..29328)
                     /locus_tag="PW224_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2907548.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(29415..32093)
                     /gene="mgtA"
                     /locus_tag="PW224_000841"
     CDS             complement(29415..32093)
                     /gene="mgtA"
                     /locus_tag="PW224_000841"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL2907549.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            32752..33189
                     /gene="ndk"
                     /locus_tag="PW224_000842"
     CDS             32752..33189
                     /gene="ndk"
                     /locus_tag="PW224_000842"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL2907550.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(33211..34077)
                     /locus_tag="PW224_000843"
     CDS             complement(33211..34077)
                     /locus_tag="PW224_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2907551.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(34237..36330)
                     /locus_tag="PW224_000844"
     CDS             complement(34237..36330)
                     /locus_tag="PW224_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2907552.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(37079..37702)
                     /locus_tag="PW224_000845"
     CDS             complement(37079..37702)
                     /locus_tag="PW224_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907553.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(37861..39258)
                     /locus_tag="PW224_000846"
     CDS             complement(37861..39258)
                     /locus_tag="PW224_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2907554.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(39290..40261)
                     /locus_tag="PW224_000847"
     CDS             complement(39290..40261)
                     /locus_tag="PW224_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2907555.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            40635..41060
                     /locus_tag="PW224_000848"
     CDS             40635..41060
                     /locus_tag="PW224_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907556.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        11983 a         8667 c         7293 g        13572 t
ORIGIN      
        1 tttttgttcg agtaaatctt ggagaagttc ggcgtaatag atcgtttttt gacagtggat
       61 actgtctttt tcaacagctg gataggcaca ctgtgcatag tggatgattt gatctgcctt
      121 ttgctcggca cgattcgcgg agattttttt ccgtgtttcg ttgatcagtt tattttttat
      181 ttttgtccga gtagatgcca atacaaaatc tggatgggga aaaagacgga tcaatgtcaa
      241 tgcgtaaggg gtcagcctgt ttgaaaagaa ctgttcaagc tctggaaaag tcaattgaag
      301 gcagttgtgt aaatccatgc gcagacgagt gattttcttc tcaatttctt gataaaaacg
      361 ggctaaatcg cgcatctcta agtaaaaatt gctttgtact tcttttggct gacgttgatt
      421 tttaagatga gtctgtgcga gccggtgggc atccgattta tctgttttcc aactgcgtaa
      481 ggtgctttct tccatctgtt ttttcgcctc taacgggttt aacaaacagt aagaaaagtg
      541 atgatcttga aagaactttt cgactggtct ggaataaatc cccgttgctt caaaaacaat
      601 caatggacgc tcaggcaagt gctggatctc ttcgagtaat gcatgaaatc ctctttgagt
      661 gtgttcaatc tcgtcttcaa ataagcaggt gtcgtctttg tataggacac tgtagctgtg
      721 tcccattgaa acatctaatg caagaatgta aggcatttgt tttcctcctt tttaagtttt
      781 gaaagaactt tactgcttca tttcgattcc aatttcctat acacgggctc atggccccaa
      841 catacgtaaa cctgattcta aaatgacagt aaagaagttc ggtttttgat agggactcgt
      901 agtcctcaag gctttgttcg aactttcttt cactcttcac tataatctaa aaatagaaaa
      961 tagaggagaa catcattcgt caatgatgtt cccctctaat cttagtatgt tttttatagt
     1021 aattccttca gatactttct tgcctcatca ttgaaacgca gtgattcttc taacttctga
     1081 taatattcca acgcttgttt ctgagcttca gtaaaacgat aatgttttcc tttccatttg
     1141 tcgattgtct cttgcacatt tccagattgg atatctttca ctactagttg ccatttttct
     1201 tttgagactg tacggtcttt cttatcagca gattgtatag cttctcgttc tttacgcaat
     1261 acttttaaaa aaccatttct gactatccga ggaatgctgt cgaagaaccc ttctgtaaaa
     1321 ccatagcgac aatattgctt ccaaataaat agatagattt ttggttggat gaatcgttgg
     1381 tttttcgcaa aatattccat ctgagcagtg acttctggtg ttcgttgacg ttttgtcttt
     1441 ctcaacacgg aaataaatgt ttcaaaataa gcattaggat gtactggcac ttcttcatat
     1501 acgatggatt gccaatcttt acgtaaaaat gcgcgatagt tgatcaagaa ttcttctaca
     1561 tcctttacta attcttctgc aaagtcgtca aatagttctt tttttatctg tcgctttttt
     1621 gactgcccat tgatgtgttc acttattttc ttgatttcat tttccaatgc ttgataatcc
     1681 gctatgtaaa tcggcatttc tacttggcga aattctgcga gccgctgagc taattttata
     1741 tccggtaaat atggctgttt catccgacga ttttggtaca agacaaaaca gtatttttgc
     1801 catagtcctt caggaaaatc gatattcttc tgcttaagcc ataataattc gtctaaggcc
     1861 agatcaactt ttgtcattcc ctgatggatc gaagctgcca ctgtcggaga gacatgcagg
     1921 tgatcggcaa agtggttgat gcctagtttt aggatctttc cggtttttgg cgatttaacg
     1981 atgtattgca cttttaattc tctaccacac tcacaataca atttttgctg gcttcgtttt
     2041 gttcgaaaaa acggattgat cttgaagtca gaaaacaccc aatctgttgc agctaaataa
     2101 tgttcttcca taaaacgaga gctgattaga tatcggcggt gcttatcgac tagttcccgc
     2161 tgttttttcg ttaattttgc atagatctcc caacgttgac tgcttgtgta ttgtttagaa
     2221 attttgatgg gatagctttt gctgatttct tttggaaacc attcttcaaa gacggattgc
     2281 cattcttcca cggctgtttc tttcctttct aattacaaaa agctggctac taacataacg
     2341 ttagcaacca gccagtgttt cttgctaaat tagtgagttg ttccggcatt tccggaaata
     2401 tcttctttat tttctactgc tgcttcaact gcataagtca aagaagaagc aatgtcttct
     2461 aaagacatag aaggcgtacc cactggacga tcgatcactt gatcgggtga gaatggcacg
     2521 tggatgaacc cacctcgaat tgccgggaat tgtgtttcga tcaaatgaag caaacgatac
     2581 atgatatcat tgcagacaaa tgttcctgct gtataagaga tgtacgctgg tagaccatga
     2641 tcttgtacat tttttgtcat tgctttgatt ggcaatgagg taaagtaagc ggctggaccg
     2701 ttttcttcta aagccgtatc gaatggctga tttccttcat tatcagggat tcttgcttct
     2761 gctagattga ttgcgacacg ttcgaacgag actgctgacc gcccacctgc ttgtccgaca
     2821 cagatcacga catctggttg gtgctgttgg atggcttctt tgactacttg actgcttttt
     2881 tcgaatacgg tcggtacttc cactttgatg atttcagcac cgctgatttc atctggcatt
     2941 ttttttactg cttcataggc tggattcact ttgtccccac caaatggatc aaaacctgtt
     3001 actagtactt tcatctgttg ttcctccgtc cgattgattt aacatatggt tatcttttat
     3061 cataccattt tccttgaaca gcgtaaataa aggaaacaat agtttaaata acataacaga
     3121 tcaaacttaa tatattctta taaaaaaata gtgttccact acaatgttag aagaggttgc
     3181 gacaattatt ctgtcacaac ccctctcttc tattatttaa atagaatcat ataagcaatt
     3241 tggaaaacta gcataaataa cgcaataaac aactggtttt tgatcactcc gtatttgtct
     3301 ttcatctcca gcatcgctac aggcacgata ttgaagttag cagccatcgg tgtcagcagg
     3361 gtcccacaaa aaccgcaagt caatgcaacc attcctatta acgctggatt cgctccatga
     3421 ctaaatacga atggtgcacc gatcccaacc gtcatcacgg tgatggccgc aaatgcattc
     3481 cccatgatta ccgtaaatat caccatgcca agagcgtaaa tgatgatgcc gatgttcaca
     3541 tttccttctg gaacgatcgt acctactgca ttggaaatca ctgtcccaac tccagcgctt
     3601 gtaaataccg cacctagaga agctaaaagc attggcaaca tgcttaacgg ccctactgtg
     3661 cccagcatat ctgctgcatc atctaaaaat gtacttggtc gattatctcg agaaaagaac
     3721 atcaagatta gcatggctgc aaaaacaccg acgcccacgc cgactaaagc acctaaattg
     3781 gtaaatagag caaacaagat cgcaaagaca ccgatactca aagctggaat aaaaattttc
     3841 attcctagtt tgtctgacat tttttccata tattcttttg atggaagccg gctttctcct
     3901 ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa agatcaagcc accgttcacg
     3961 attgccggaa gccatcttcc tcctgcaata actaccccta agaccgtcca gaaaaaggct
     4021 gacccataac gatgaggatt ggttttatct gttaaatttc ttactccagt gtaaatcaat
     4081 acgagtccca tcacgatata aaggatctcc agtaatttat cgcctaataa aatatcagga
     4141 tttgtaaaaa agtccatctt attgcccctt tcctggataa tatttcttcg ctaatttctt
     4201 ttctttaaga tagaagtaga cacttgccac aactaaagcc gtgatagcca ctggaacttc
     4261 cactaaagca agctgtccca atgttacgtc atatcctaga tctttcagtg ttgattggac
     4321 tagcaacgcc cctgctcctc cgatgaatag tacttggcca aagaaccagc agacattttc
     4381 catagctgaa gccatacctt ttagttcttc ttcatgctta ggatggactt ctaacccttt
     4441 tgattcgatg gtccccaatg ccatcggtaa aatgattgga cgaacaaatc cagcaactcc
     4501 accaaaactc acgttaaaag ccgcaaagat cattcgaaag atcccataaa tatctacaat
     4561 cataccagca gatactttct tgaatcgctt gatcaaggaa gccgctgatt ctttcagacc
     4621 atttctttca agcgttcctg ttgccaacat aatgatgata aaaatcgcca tcccgcgatt
     4681 gtttacaaaa cttgtaccta aaagttctaa cattccttga atgccaagac cgccagtcaa
     4741 agctgtaaca actaaagcaa ccattacgat caatatcgag tctaatttaa atgcaaaccc
     4801 gagaataaca atcaagacgc ctaaaaggct aagataattt tccatatatt ttcctccata
     4861 aaatgacaat atttttttaa ttataaagca aaacacataa aaaatatagt tgttttctca
     4921 cgaaatagac actattttta aatatttttc gttatttttt tatcacaaaa attatcttac
     4981 ttattgtaag gtaaagcttt ttttaattta ttgcccgata atatatagaa ataaggtcta
     5041 gttactgttt tttttaaatg attaataaat aacaacttct ttctatgaaa ttgcaaagca
     5101 ttcatcttct tttccgctaa cgatatatcc gtcagtgctt ggtacacttt actaaaaagt
     5161 atgagattgc cgatatctgg ataggtgtgc tatactacag aaaaagatat acaatctgcc
     5221 accgggtagg ataaagagga tgatcattcc attaggtctt tgaagggatg attttcctct
     5281 ttttgtgtat ttttttatag ttaaggagga atgtaagtat ggcatggatg gaattaatca
     5341 tagcaggaat tttggaagta ttctggtcaa cgatgatgaa atggagtgat ggtttttcta
     5401 aaatcaatta ttctttttac acagtgattg gcatgattgc tagtttttac tttttgtcaa
     5461 aggcaatcaa aagtttgccg atgagtctct cttatcctat ctggacaggg attggcgcag
     5521 ttggttcggt cataattggt gtggtcttgt ttcatgataa gctgaatctt tccacttggt
     5581 tctttgtggg gatgctactc atcagtatca ttggcatcaa gattactagc ggccactaga
     5641 cacttggaat aagtactgat cagaaaaaat aagccttttt atttaatatt caattttttt
     5701 atgaaaaaat aagaaataag tccaatgacc tagcgacttc tccactcatc aggtgtatgc
     5761 ttgtactatc tatagaaaag gagtgaatat ttttgaaaag tctttcggtc caagaattat
     5821 ccaagaactt tggtcacaaa caagccctca gcgatgtcag ttttagctta tcccctggca
     5881 aaatcgttgg attagtaggt cctaatggcg ctggaaaaac aacgatcatg aaagctattt
     5941 taggactgat ccattactca gatggaacaa tctgtattgg tgaaaatgag atttctccct
     6001 ctagtcataa agggttggaa caagtaggag cattgatcga atacccgggt atctatccat
     6061 tcttaacagg ctatgatcat ctgaagttat tctctgaaac caacgatgta tctgctatcg
     6121 acactatcgt gaatcagctc aagatggaaa agtacattca caaaaaagcg aaatcctact
     6181 cgttgggaat gaaacaaaaa ttgggaattg ctcttgcctt actgaaccat ccgtctatcg
     6241 ttattttaga tgagccgatg aacggtttgg atccacaagc aacaagagat gtgcgaaaaa
     6301 tcattaccga cttggctact cagggcacct ctttcttgat ctccagtcac ttactcagcg
     6361 agcttgaaaa aatggtcgat gagctgatcc tgatcaatca aggaaagatc atcaaacaat
     6421 gtacaatggc tgaattgaac caaacagcgc aagatttcat cgttatctct acaacaaatg
     6481 atctagctgc tcgaacgatc ttgacagaag ctggatatac tttggaagat cctgataaaa
     6541 tcaagctttt aaaagaaaac gaagaattat taggaaccgt gatccagcta ttgtctgcac
     6601 aaaatatcca tgtgacagac gtccagcatc tgacgaatga tttggaatct tctctattgg
     6661 aaattctgga ttcagtaaag ggggaagaag aaaaatgaca ttgcttgtaa aacaagaatt
     6721 atttaaattg atcaagaaaa agtcaactgc tgttttatct gttctattag ttgttttgct
     6781 cataggaaca gcacttctag ccaaaaaata cacgacgatc attgatccag ttgaaatgac
     6841 tgctcagctt tttagtgcca catcttggat tgtctttatc atgattgcag ctgcaagcac
     6901 gatcatttcg atggaagcac aatatggtac attgaaaaat ctcctttatc gaaagtattc
     6961 tcgaggagaa atattagtaa gcaaatggat cacattagtt atttattctg tttatttata
     7021 ccttttagct attattgtca ctgtgttgat gaaactgatt cttttccctt cgatttcctt
     7081 cacggagaag gtttctactg gtcaaacact gatccaaagc ttgttcactt atactttggg
     7141 tagttatatc ggtttatggc tgattttaag tttagtgttg atgattgctt gctttatcaa
     7201 tagttccggc gcatccatct cagcaggaat cgtcttttat tttgcttcgt caatcatatc
     7261 tggtatcctt attgcactca tccaaaaatg ggagtggatc aaatggaatc cgatcagtat
     7321 gttgaatctg caaaaccaaa ttggaaacga agagatcatg aagcaattga ctcatctttc
     7381 tactaatcaa atgctattcg gtaatttagc ttacatcgtt ttgttcttag cattaggata
     7441 tctcgtattt aagcgaaaaa atgtatgatg actcatcaaa aaagaggctg tgaaagagac
     7501 gttcagcctt attgccgatt ggctgtctct taaacaccgt ttattctgaa aaaggagctg
     7561 tgacaacatt tgtgtcacag ctcctttcct agtatgtttt tgaataaacg acaggctcac
     7621 aacctctctt tatacttctt ttatacttct atacgtgaag catcccagtg gcaaataaaa
     7681 ccattgcaat cactccgacc atcgtcacac ctataagtaa aatcttatct aagtggatat
     7741 gttgtacagg ctcgttttgc tccttacgtg ctttgaggta aaaataaatg cctggcaaga
     7801 aaagaacaag aacaagcatt acttcattaa aggctacgac tagcatagcc catacttgaa
     7861 aggcacttgc taaaagccct actaaaaatt ggacccattc tttccgctga taagaaagtt
     7921 tcatttggta caatcctaag aaaagccacg tgaagaaaat agctgctgta ccaagtgtgt
     7981 aagcaaagtt ataagcactg tcagtgaata ataatgaaag taaaaagaca ttcgtacata
     8041 acgtcgtcaa tacgatagca taagttggtg ccccattttt gttgagtttt ccccatgctt
     8101 taggcaaaag tccgtcttca gccatgtttc ccattgtttc aatcggtaag atcgtccatg
     8161 agagccaaac accaactaaa gaaatgatca aaccgccgtt gatcacgact gctccccatt
     8221 taccaaccat catttcaaac acatagccca tagcaggctg cgccttgatc gtttgcagct
     8281 gattcatcgt cattgtccca tatggaagca tggaaaccaa gacgtaaata ataatcaagc
     8341 cgaatatccc tagaatcgtt gctttcccaa catctgattg tttttttgct cgtgaaccta
     8401 cgacagaggc tccttcaata ccaacaaata cccaaagcat caccatgatg caactcaata
     8461 cttgactgga aaacgggatc ggctgtccat tcgcattcaa tataatgtcg ctagaaaagt
     8521 tatcaatgaa ttgttggatc gaaaacgtgc tgatagctac tacaataaat aaaaataatg
     8581 gcactaattt acagaatgtg acgatagcat tgacgaaact agcattttcg actccccgat
     8641 tcactagcca tgcatatccc cataaaaaga ctgtactcat cacgatcgat gccagatttt
     8701 gcccaccttc aaatactggg aagaaatatc ctaatgcgct catcaacaat gttgcaaaag
     8761 ctacgttgcc taaccagcat gtgagccagt aaccccaacc gctgataaaa ccagcgaaac
     8821 ggccaaaacc ttcttctgcg taactaaaga tcccgccttt taaatctgga cgttttttat
     8881 tgatattatt gatcgacaag gagagcatca acattgctgt tcctacgagt acccaactga
     8941 aaatagctgg gccgccagcc gatccggaga tgtccgtcat aataccaaat acgccacctc
     9001 caatcgctga accaattact aaggcagcaa gtggaattaa accaattcct tttttctgtt
     9061 gctctgtatc catgtaagca tcctccaaaa taaaaccgct tttatcaaaa agtataacaa
     9121 acctttttta tttttactta attaataaaa actttaatag aaatataatt ctaccggctt
     9181 ttttctcatt atacaagcaa agcgcctagt ccctttccaa attacaccac caaaaaactt
     9241 aaaaaagatg ctatactaaa aggaaagaat tggaggatga tccatgaaca ttttatatat
     9301 atcgatttca ggaaatacgc gtgcctttgc caaacatcta gcagaatatg ctgaaaaaat
     9361 gcatgcagaa gatcctgtca atcctgaagt tactttaaaa gaaatccatg aaaatagtga
     9421 ttttacgaaa gaaacagaac cattttttac tttcgttcct acttatttag acggcggaaa
     9481 tggtttagat aacggtgata cagaaatctt gaccgaaacg atgcgtgaat atctggaaca
     9541 tgaagacaat cataaacttt gtcttggtgt agtcggtagc ggaaataaaa acttcaataa
     9601 tcagtattgt ctaacagcca aacaatatgc acaaacattt ggctttcctt tcttggcaga
     9661 ttatgaatta cggggaacac catctgatgt gacacgaatt tatcagattt tagcagaaaa
     9721 caataaataa taaaaaagaa gaagctgaaa aatagatttc agtttcttct ttcgtttcta
     9781 ttgtttctat catattaagc tttgacatcc atcacaggtg cttttgcttt tgctttacct
     9841 gttttttcca agacaaagtt ttttacacga tccatattgg ttaccaagac gatcatggat
     9901 gtttctttgc ctgctgcttt gatcgcttct aagtcagctt ctgctacaag cgtatcaggt
     9961 gtcacttgct gcccttctgt tactttcaca tcaaatggcg taccgcctaa gtcaactgtg
    10021 ttgattccca tatgaagcaa gatttctaaa ccatttggcg ttgtgatccc aatagcgtgt
    10081 ttcgttggga agacagagct gactacccca gtaactggag aatagatatg tccgttttct
    10141 ggttctacaa agtagccttc acccatcatc ttttgtgcaa aaactggatc acttgcttta
    10201 tctaaagaaa caactgttcc gtcagcaact gatgagagga tagtcacact ttcagctgaa
    10261 gtttgaggta ctactttttc aggcacttca tttactgtgg ctgtttcagt cgctaattct
    10321 tctttttcta aacggtcttt aggaataccg aagaagaatg tagcaacgaa gccaccagca
    10381 taagctgcca ataatccaag gatgtagcct aaccatttac cgtcagagat caatgggatc
    10441 aaagcggctc ctgaaggtcc aatagcgata gccccgacat ttccgatcat tccgatcact
    10501 gccccgccga tacctccacc gatacaagca gtaataaatg ggcgtcctag aggtaatgtt
    10561 actccgtaaa tcaatggttc gccgattcct aggatcccga ctggtaaagc tcctttgatc
    10621 atttctgtta attctttatc ttttttgcag cgaatccata atgccaatgc tgcacccact
    10681 tgtcctgcac cagccatcgc aagaattgga agcaatagcg tacgaccagt ttcatcaatc
    10741 atttgcacat ggattggagt caaaatttga tgcagtccga acataaccat cggcaagaat
    10801 aatgtgccta gaacgaagcc ggcaaatgct ccaccaacgc caagtaccca gttgatccca
    10861 ccgaccatgc catcagaaat gaaacctgct aaaggcatga tgaagaaaat ttcaatcaga
    10921 ccgatggcaa tcaatgaaag agttggtgta acgatgatat ctaccgcatc aggtactact
    10981 ttatgcagtt ttttctcaac gattgacaat agccaaacag cgaagatgac acctaggatc
    11041 ccaccttgtc cagctgcaag agcttcacca gtaaataagt ttttcagtgg tgcttccgga
    11101 ttcatccctg tcagcatcac aacggccccg ataactccac caagagttgg tgttgcacca
    11161 aaaacttgtg cagcattgat acctgtatag ataactaggt agctaaacat cccgtttttc
    11221 aagatgttca tcacatcgat atattgttgc catgttgctg catcaagtgt tccggctgtg
    11281 actaagtttg ctaaaactgc ggcaatccct gccacgatcc cagtaccgac aaatgcagga
    11341 atcattggca caaagatatt agaaatgtct tttaataccg atttgatttt agatggtttt
    11401 tgtttcgatt tttgtgccgc tttcatttct tgtgcccgtt cgtttacacg gtcttttcca
    11461 gatgctgatg aatttgctgt agctgtactt acagaagaaa gtttttctcc taatttcacg
    11521 cctgccaaat caaccatttg ctgtgctact ttgttcactt tcccaggtcc gatgatgact
    11581 tgaagctgtt catcttctac tacaccaagc actcctggaa tcgcttttaa ttcagagaca
    11641 tttacaagat catcattgat gatcttcata cgcacacgag tcatgcagtg gacaatgctt
    11701 tctacattgc ccatgccgcc tgccccttta taaatttgtt ttgccagttc ttccaaagtc
    11761 agttcgtttt ttgccatggt cttttccccc tcataaggtc ccttttacaa atccgtttgc
    11821 ttcagctaat tttgcttgag cttcctcttt atcactattt gtcaaaatca tgacgatagc
    11881 cagcttaaca ttttgatctg cttcttcaaa gtacttttca gctgtttcgt aatcagcttc
    11941 tgtcgcttgc atgatgattc gttttgagcg ttcgattagt ttttcatttg ttggtttgac
    12001 atcgaccatc aagttattga atactttacc tataccaatc atactgatag tagaaatcat
    12061 attcaagata agtttttgtg ctgttcctga cttcaatcga gtagagcctg tcaaaaattc
    12121 tggacctgca tcgacttcga ttgggaaatc cgcgtgttta ctgatttcgg cttgtttatt
    12181 gcaagaaatc gttcctgtga cagcaccgat cgattttgcg taatctaatc caccgatcac
    12241 atatggtgta cgaccgctag ctgcgatacc aataactata tcattttttg tcaaattcag
    12301 cttgattaga tcttcttgcc ctagctcata agaatcttcg gccccttcga cagctacagt
    12361 cattgcttct tctccaccag caattagacc tatgaccatt tccggttcta caccgaatgt
    12421 cggtacgcat tcagcagcat ctaacacacc tagtcttcca cttgttccgg ctcccatata
    12481 aatcagacgt ccaccttttt taaaggactc aatcgtgtta gcaatcactg gttcgatact
    12541 tgtcagttct ttttcaaccg cagcggccac tttctgatct tctttgttca ttagttgaac
    12601 ggcttgagaa atactcatct catctaatcc aaaagtttct tggtttctgc gttccgttgt
    12661 taggttctct aaattgattt tgctcatttt tatcttcctt cttttcttat aaattgatag
    12721 ttcataccag ctttgatttg gttgatcaat ggtagctctt ctttgataac tttagctact
    12781 acattcactt tttcatccgc tggcaaatca tatttggtta attggatctc acccatatag
    12841 cgcaaatact tttcgttatc taaagtaata gaccctttca tacgcgtagc tgtatttctt
    12901 gctgggatag gcggaatctc tcgaaaacgt gcatctacgc ttcgtatgac atctcttgca
    12961 tcgtcttgcc gattcgtatg cttccctaat atatattcgt aaaattcttc atctacaggt
    13021 tccgtatgga gcaagactgt ctgttctttt tggaaagaag caaattgctc ctgaacgttt
    13081 tctgacagac ctgcatctcc gatgtaaaca atatccgtgt tacttgctaa tagatctaga
    13141 gcgcctgcaa gaggatggat ccctcgatgt tcttccaaag ttggcaatcc ttgatatagt
    13201 gggcctctca actctgtatc tcctggtaca aaaccttgga caaggagacc ttgaattttg
    13261 agccattgat tttttttctg ataccattct ttgtccagcc ctgtttcggg acgtggataa
    13321 taattatgcc aagcttcaat ctgtgagaaa tcagcttctg cttctttcaa ttcatcgata
    13381 tctttaggcg tgatcgtgct agcattcaaa ctgattttca attgatgtga ccagtcggca
    13441 atctgctgat tggaaatatg ataatccatt cttaaacctg ttacaccaat tgctttcaaa
    13501 ggcgttagat cttctaatga aaaacccgct cgttccaatg cctctcctga aatatcgacc
    13561 atcaattcta gtttattctt tttcgcttcg cttcctagtt cgatcaatcg cttttgatat
    13621 gccgaaacat catcttctgg tatatgcatg gaagtgaaaa taccgacaaa gccattggat
    13681 gccatttggc ggatatactg tttcttctct tctgacaaag aatcgttcat aaatattgaa
    13741 aagccaaaca tattatccct ctttttctgt atccgtcttc atttctatga ctagagtata
    13801 tatctaaaga aacaaaattt caatactttt attaaaataa ttaaatttca tttctaaagg
    13861 ttgagtttat attatcaaaa gaattgtcat aacctaaaaa aggcactgct aagcaaaagc
    13921 agtgccttga aatattttat ttaaataaca caaaaacata attactcata agaaaccaat
    13981 tctgtattat ctaccatgaa tgtaaagaac ccctcattag gcatcggatg aaaataagca
    14041 ttgatcactc ttatcaaggc attatggcaa actaacagat agacattttc ttcgtcttcg
    14101 atgcattctt tcaataaagg aacgatccgt gcatacacat ctaagactga ctccccattt
    14161 gggaatcgta cagcgaacgc taaacgtgct ttttgaaagt tctcatcttt gcttggcagt
    14221 ccatcataat ccccaaaatc catttctacc aatcgttcat caacggtcag cggcaagctg
    14281 agacgatcag caactgcttg tgctgtatct cttgctcgtt gaagagggga atggatgatt
    14341 ttcgtgattg gaacaggcaa atctactacc ttttctgcta gtttctgtgc ctgcatctgt
    14401 cctttttcag tcagcggcac atctgcccgt ccgcaaacta atccttgtgc gttccaagtt
    14461 gtttctccgt gtctcgttac ataaagcatg tgattctctc cttcgttggc aaagttcccc
    14521 ttaaccatac cttgttgttc ttgagattac aagggttaga ttctctcaag agaaataatc
    14581 tcagtagata ctttatccag gaaggtttga tcatgttcca ctagtaacat ggcaggtttc
    14641 acgttcaaga tcagttgttc taattgttct tgattgaaga catccaaata attcaatggt
    14701 tcatcccatg tatatagttc agctggctgt gataaagatt tagccaattc cacttttttc
    14761 cgttggccca tactcatctg ctcgatcttg ttatgaaaaa catctctttc catcccaagc
    14821 tttcggaggt tgttcaaaaa tgcttggtag tctacttggt ttttctctgc aaattccgcc
    14881 aacgttcctc gattgtcttc ataattttgg cttgcataac taatgctcag atgttttggc
    14941 tggtattttt ctcctataac cttgccgttg aatgccccca gaagataatg gataatggat
    15001 gactttcctg caccgttagg acctgaaatc gccacccgct gatgaggctc gattgtaaaa
    15061 tgaattggct cgaataacag attttcatac cctaattgaa gatcttctac gcttaaaagt
    15121 cgcttatggt gagacgcttg gctattcatc gtcaaagaat cgatatattc gatatctttt
    15181 aatagttttt ctttctcgct gatctgggtc tccatccgat tcacgattgc tttggatcgt
    15241 ttcatcgtcc gtgcagcatc agcacccact gctcctttat tcactcgtct agattcagta
    15301 tcgatgaatc cgacttgttt ctttgtttta tctccttctc gggaacgaga ccattcggct
    15361 ttttcagctg ctgttttctt tagcctactg acttcttttt tcaatttttc attttgagcc
    15421 atttcaaatt catcacgaag ttttttctgt tcttcataga tagagaaatt cccttgataa
    15481 agttccagtt gacttttttc gattgctaaa acatggtcca ctacttcatc gataaatccc
    15541 cggtcatggc tgaccacgat gaagccttgt tttttctttt tcaaataagc cgctacttgt
    15601 tttctaccag agatatccaa atgattcgtt ggttcatcga ttaacgggaa atgagtgtca
    15661 tccacaaata aaagtgccag taggactttt gtcttctctc cccccgatag tgtgctgaat
    15721 tctctccata agatttcagg atctgtttgc atcaattgga gctctctttc gatttcccat
    15781 atctcaaaat ccgtaatatc atttaacacg taataggtta aacgttcttt atcttttgtc
    15841 tgttgcggga aataggcaaa ttcttgctga tggattactt gcccttgata aggtagtttg
    15901 ttttgtagaa tattcagtaa agttgtcttt cctcgaccgt ttcgtccgat aagtcctagt
    15961 ttccattgtg tgtcaaaatt tagatttgct tgttcaaata ataatgtgcc ttggctgtcg
    16021 tagccgaatg tcagattttt tatttcgatt ttagacattt tccatcacct cgtaaaaaaa
    16081 gaccagtcct attttcagaa ctggctaaag aggatgcaag ggttggatac acacaaaaag
    16141 acccatacat cgaaaaaacc aagccaatcc cgaaaatctg cacactgctt ccttgtctcc
    16201 acaaggtaca gtctgaatgc agctttcatc aatcgactta atttttcaat gcacggatcc
    16261 ctcactttgt cctatatttg aaattacgat agcataaaga aaatgatttt gtcaactact
    16321 tcgggaggat ggtgaaaaat ctgctaagtg tctgtcctaa gatttttgtt ttttctaatg
    16381 tttgcctgtg ttccattgct tcttctaaac ctattggacc caaatgaata cttaagacaa
    16441 ttgggagaaa atcagaaagt ccttctagct cttggtctct acttccgcaa atagcgatta
    16501 ttggttttcg atattttttt gcaagcactg ctacaccgta tggaactttt ccgtggatcg
    16561 tttgctgatc gatccgccct tctcctgtaa tgatgacatc tgcttgttcg atcttttttt
    16621 ccaatccagt gatttggctg actaaatcga aaccagaaac catcttcgct cccaacaaaa
    16681 aaagggctcc accaataccg cctgctgctc cacttcctgg aatatcatct aaatccacct
    16741 cccagaaatc ttttatttgc tgagcaatct ttcctgcttg tttatctaag aaagctagcg
    16801 tcgtatctat tcctcctttt tgtcttccaa attgccaagc agctccttgt tgacctgtat
    16861 atggattggt aacatctgca gcgataatca attttacttt agaaacaaga acgtttcttc
    16921 gatcaaaatg aagtttttta gtagataata aaggatttcc ttctgtgtac ccttctaaag
    16981 aagcacccaa ggattggagt aatcctagac caccatcgct acacccgctt cctcctaaag
    17041 tgacaataat ttgttctact tcttgaagca gtgcatcttt aataagttct cctaaggcat
    17101 aactggaagc aagtcggata gtttgatccg ttggtttatc aagtagatct aaccccaatg
    17161 cttgtgcaga ttcgatgact gcggtgtttt ttccttgcca agttgtcaat aaatagctga
    17221 tttttcgctg acggaataag agatccatcc catcaatggt acgccatgtt cccctgagcg
    17281 cgtaatagat tgcttctgca gtattttctc cgccatctgc aatcggcaca ttgatttttc
    17341 ttcctttacg atcttcccag ccatttagaa ctgcctgatt tagttcaaat gaagaagcac
    17401 ttcctttaaa tgaatcaatt gccgccaaaa tatccatctg tatccctcac tttcttctta
    17461 agtaagacac tttttttcat ttttttcaaa caaaacaatg ctattttctt ccatttttct
    17521 atatactaaa agggaactca ttgaataaag gagagaaaaa acatgaaaat cgcaatagct
    17581 ggggccggag caatgggcag ccgaatcgga ttgatgctac atcaaagcgg aaatgaggtc
    17641 ctcttgatcg atcgttggcc tgctcacatt gaagccattc gtacaaatgg gttgattgct
    17701 gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga agaaatcatc
    17761 gaaagcaacg aacacgtgga cttgatcgta gcattaacaa aagccaatca attagatgat
    17821 atgttttgtt cgatccaatc cattattact gacaatactt atgttttgtg tttactaaat
    17881 ggattaggtc atgaagatgt actggaaaaa tatgtgccaa agaaaaatat cttatttggg
    17941 atcacgatgt ggacagctgg tctagctggt cccggaaaag tcacattgtt aggtgatgga
    18001 gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa agttgtagaa
    18061 gtctttcaag aagcaaattt aaatccgatc tatagccata acgttcgtta ttccatttgg
    18121 cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca cgattttgga ttgtaatatt
    18181 gctgaactag gcgcgcaaaa agcggcagag agcatggttc gaacaatcgt atctgaattt
    18241 gcttctatcg cagcaaaaga aggaatcata ctagatcaag aagaagtgta ccaacatatt
    18301 gcttctactt atgatcctga caacatcggg ttgcattacc catctatgta ccaagacctg
    18361 atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg gtgccatttg gcgaaaagga
    18421 caaaaatatg atattgctac accatattgt gcctttttga cccaattagt ccatgcaaaa
    18481 gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag aatcagctga atcagcagct
    18541 ggttctcttt ttttaagccc aataaaagat caaaaagaaa acacaactct catgatgcca
    18601 taagaattgt gcttgtttgt ttcagtacat aatctcttca aaatgtaggt tttttatttg
    18661 ttttcaacag aggagaggtc atattcgtat tgctcataaa tagtggaatc gttagtgata
    18721 aatcgttttg cccaattttc tgcatcataa aattccaata gctctgatag gattggctta
    18781 aatggtgtat gcagaaattc cggtaattca tctaaatcac gttctcgaag caaaaaaata
    18841 ttttcaggac gataaaaatg ataatgagca atcgcttgat tatccgtcac tagtttcttt
    18901 tgaaaataca tggattcaaa tgtccgcaaa gtcactcctt gctgaccctc ccgcaacagt
    18961 tccagaatcc cgcgcgattc ccctgttttt ttcaggtact cttcataagt aagatatcca
    19021 ggttgttcat tccctcgatg tggaagaatg aaatagttgg tcgtaacatc tctttttata
    19081 aattcctgct tatagctttc tgctcgttct tttctcccat tattcgttgc accaaaaaat
    19141 agatccgtct ttggttcgct agaagaaaaa ttcatatgtt tcgaataaaa tgaactatta
    19201 tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat gataaatctc atcgattgcc
    19261 ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg attccaaaaa
    19321 aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc catatcaaat
    19381 ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtctagc tgatttaatg
    19441 aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat ggaaggcaaa
    19501 aaataaagtg atttattttt tgcaactagc agtacgtcat ttttgttcat cttttccgcc
    19561 tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa
    19621 cgtttcctat atttgtatga aaatttaaca tttgtatgaa aatatgatga aataaatcat
    19681 tcttctatta aaattcgcct aaaagttttc tgaatagttg taattgtctg aaaatttgtt
    19741 atcattagtc attgttaaat attgctattt attcttaata taaattttga ttgtgaattt
    19801 aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag ctccagacat
    19861 atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa
    19921 gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggtgg gctgatttta
    19981 tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac gcatcacgga
    20041 ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac tgattgggtc
    20101 tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc ttcctttttg
    20161 cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc tgttttaact
    20221 acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg gctggctttt
    20281 gctaaaatca gtgtcattat cttactgatc atttttgcta cttatcttgt cggtcagcaa
    20341 atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg tgggtttact
    20401 cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc ttacggcggt
    20461 tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa
    20521 gcaatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt gtttttgctt
    20581 ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt tgtgatggta
    20641 tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgtgat tgtccttgct
    20701 ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg
    20761 aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca agtaccgcaa
    20821 cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc ttattttgtc
    20881 ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt attgatctgg
    20941 atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt atgggataaa
    21001 ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat cctgttcacg
    21061 aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta tttcagttat
    21121 caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa aattttattt
    21181 tatttatgtg gaatagatgg agctattgaa gaaaggagtt gtgacaaaag tcttgttgca
    21241 actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc aaaaaccctc
    21301 tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag gaatgaaaaa
    21361 aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac
    21421 ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag
    21481 tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca tcatgctgtt
    21541 gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag ccgctagtct
    21601 tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca acgacacagc
    21661 ttggatgaag gccatgattc ctcatcattc tatcgctatc ctggcaagta aacgagctga
    21721 tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac aagaagaaga
    21781 gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg tccaaataaa
    21841 aaaagaggat gttccagaag ttatcagctt caagaaataa gaagaagcta cttctggaac
    21901 gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
    21961 aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga
    22021 acaccgttta ttaagaggtt gtaaaatcag cgttctgacc tgagtattag agagacaaat
    22081 ggaaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt cagctgattg
    22141 aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta agaaaaattt
    22201 tgtgaaatgg ctttccacat ttttgtataa atttggctta ataccgagaa actggctttt
    22261 gaataccgtt tattcgtaaa agggattgtg acaaaagtct tgtcacaatc cctttttatg
    22321 tttttattgt tctaattgcg ctgtcttgct tactgcttct tgcggtttca caaatttttt
    22381 tgagattccc caacctgtga aggcactgac catcgaaaac acaggagaaa gcaaactgaa
    22441 aaatacgaat ggcaagtaag tgagtgtagg aataccaaat gtacttgcta cgaaacttcc
    22501 ggcaattccc caaggcacta agtagttgat tactgttcca ccatcttcta gcgttctacc
    22561 taatacagtt ggatcaaatc ctgctttttc ataagtttct ttaaatgcat taccaggcag
    22621 aataacagat aggaattgtt caccaataaa aatatttact ccgatacttg tcatcaatgt
    22681 tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat actcgttcca tcacggttga
    22741 gatcaagcca aattcgatca acaatccacc taatgacaaa gttagaataa tcaatgacac
    22801 agtaggcatc atactttcga ttcctccgcg gctaagcagt gaatcgattt gttggtttcc
    22861 agtatttgaa acaaaccctg attcgatgac cccagccaac ttcgttaagg aagtttgtgg
    22921 gttttgaata aaaatcattc ctactgctac agcgatattc aacaaaatcg ttaaaattgc
    22981 cggcaccttt ttccatgcac acagtaacat taaaaataaa ggaatcagtg accagattga
    23041 aatagtaaag tttgcttcta gaacatcagt cacttcacga atagatgaaa gacttgcggc
    23101 tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg ccgggacagt
    23161 tgaccacatc atatgcttga tgtgtttgaa caaatcggca tcaacaatag ctgctgctag
    23221 atttgttgat tcggataatg gagacatctt atctccaaaa acggcacctg aaacaatagc
    23281 tccaacaacc agcgctggat ttaagcctag tgttgtccca ataccgaaaa atgcaatccc
    23341 aatcgttgac ataacagtaa aggcactccc tacagcgcta cctacgattg cacacacaac
    23401 aaatacagaa gggacaaacc actggacact gatcaatttg aacccaatga ccattagagt
    23461 tggaatgatc cctgcttgta tccatacagc aatcaatgaa ccgactaaaa taaaaataaa
    23521 gattgggatg attcctggtt tgatgccatt gatgattcct tcgtgcagcc aatctgtcgg
    23581 gtatcttttt atcactgcat acaacatgac cattccgatc gctgctaaga tcgtgatatt
    23641 aggtgagacc tttaatccga caacgccaat cgcaatgcct aaaacaataa ccattaatac
    23701 aagtgttgct tctaaaaaac ttggtgttcc tttttttccc atgataatct ctcctttttt
    23761 agttgttctt atgttctaaa aaagttaaga tatcggatta ctccgccgca attcatccgc
    23821 gattcctcct ttataaaaat aaaaagacct gttttaaact aaggacgaat gactccgcgg
    23881 taccacctta attaaaaaca gatctgtttt tcactttcaa acttttattc aaatgtatgg
    23941 actttccatg taattcgtca attaggcttg atcggttcac agcgaccacc gactttctga
    24001 acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt acgaagttta ccttccataa
    24061 gatcatttgt caatgaaaaa gaaaatattt tttcattttt ctgaaaatta tttttataac
    24121 tttcacacac aagattattt tttactagaa agtagcgccc tcttgttttt ttcttgctac
    24181 tatttcttga tgttcttgct tgattctttc taatagcttt ggctctttca acagatcaag
    24241 agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt
    24301 agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt
    24361 aggctggatt gttggtatga cttggctgac atttcctaca tcagaagaac cgagacttgt
    24421 tctttgttcc gtttctattt cttcatctgg aacacctgct tcttttgcat gctggaagaa
    24481 taagtcatca aaagaaggag taacgatgac atcatcaact gcattttgga acaatccaaa
    24541 ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg atattttcca ctttttcata
    24601 tactttatct aatgtttgcc gatttcctgc acgtaaatag aaacgtccag cagcgtaatc
    24661 tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc ccatggatat tgacatcttt
    24721 tggcaattgc aagcgtagag cgttgattcc attaaacact tggatcaatg cttccaaagc
    24781 atttactcct ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc
    24841 gactggatca ttcgctaaag attctgtcgt tttcccataa cgaaaagctg gatggacgca
    24901 taacgctaca tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc
    24961 gttctcgccc ccttcttccc ctggggtgcc atatacacgg atttctccac cagtttcatc
    25021 aatgaattgc ttcactatgc tggcggctaa tacggagtaa gtaccaaaca aattatgccc
    25081 gcatgcatga ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc
    25141 gggtttattg gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc
    25201 tacccaaaac ctttcttttt ctaattgttt aatcaacgct gctgaggaat aaaactcata
    25261 gttacttact tcaggatgtt cgtggatatc tagagcaagt gcttgatatt ctggtaaacg
    25321 atattggatt agatccttca attggtttgt tgagattgtt gttgtcataa aaaattcctc
    25381 ctatgctagt catttttctt attttctccc gaatgtttcc catgcaggca cgcttgatcc
    25441 ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt tgataagcgt ctactatttt
    25501 ttgatacgtt ttattgtttt catcttcttt tcttgcaacg atgatattca cataaggacg
    25561 tgcatggtca tccactggtt ctaagaagat ggcatcagaa gcaggattaa atccagcatc
    25621 tacagctaca ccgctattga tcactgaaat gtctacatct gacaaagcac gtgctgtttg
    25681 ggaagcatct aattcagaaa tgtctagatt ttttctattc tcggtaatat cagaaacagt
    25741 gggcgcttgt ccttttgcct tatccacttt gatcaatcca gcagtttgca ataacagaag
    25801 agctctgccg ccatttgtaa catcattagg gattgctact tttgctccgt ctttgatatc
    25861 agctacatcc ttcaaagtgt tagaataaat tcccagcggt gcattgacag tatttccgat
    25921 cgatactaaa tcagttccgt gttctttatt gtaattgtct aaaaagaatt gatgttggaa
    25981 agaattcaaa tcaatcgatc catctgctaa agccacattt ggttgattgt aatctgtaaa
    26041 tttcacgtat tccaaattaa tgttctcctt tttcaaacgg tctttcacgt tgtcccatac
    26101 atctgtatct tctccaataa tccctagttt tacgatagtt tcttttttgt cagttgtatc
    26161 tgcttttcct tggctacacc ctgccaacca aataattgct acaaatgtaa tgactacaaa
    26221 acgaataatt tttttcattt tttctcctcc actttcaatg tgtaattttt ttaatcagtt
    26281 gattactgat aaattgactt aaaaaaacta agattagaat caataatgta gccacaatcg
    26341 ttacatctgt ttggaatcga ttataccctc tcgatatagc aagattccct aatccgcctg
    26401 cacctactgc tccagccatg gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga
    26461 cccgaatgat accaggcaaa ccttctaaca aatacacacg taaaatgata ccgattggac
    26521 tggttcccat cgcttctgcc gcttcgatca ccccaggatc aacttctagc agtgcgttct
    26581 cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa gctgctttcg
    26641 caccaatcgt cgttccaact aagaaacgag tagtcaatgc taaaagagaa agcaagataa
    26701 tgaaagggat ggaacgaatg atattgatca gcttatcaag tatttcaaat agcagacggt
    26761 tagctagtaa tccattaggg cgtgtcacta ctaacgtcac acctaagatg attccaatca
    26821 tcccgccaat gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa
    26881 attcatccgg aatcaaataa acattgggca aatatgtttt aaagaaatca gtcattttat
    26941 cttcctcctt cgatcacttt gatacgacta tctaaatcgt ttttttgtaa aatatttatt
    27001 tttacaccag atttgatcaa ataatccaat gcttttttcc gctgtttggt ttctccagct
    27061 aaagtaacga tcaaactccc taacggcgtt tcttgtaata actctacatt gccatataaa
    27121 atattcgctg ttacttgata tttgctgtac agatgagcga ttagaggttc gttcgtctga
    27181 tttccaatat aacttaattc caccagccat ttattggcga tctgatcggc aaaagcagaa
    27241 tgggcaatga tcgtttctaa tgcttggtca agatgagtag ctgttcgaat aaaatctttg
    27301 gtcaattctt cttcaggatt gctgaagatc tggatgctgc tacctttttc aacgattctt
    27361 ccatcttcca taaccgctac cttgttgcag atttctttga caacttgcat ctcatgagtg
    27421 atcaggacga ttgttaaacc taactggcga ttcaattttt ttaacaatgc aaggatctga
    27481 agggtcgttt tcggatcaag tgcactggtt gcttcatcac ataataatac cttaggttca
    27541 ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag ttgtttagga
    27601 taagcagatg ctttttcttc caaaccgaca agttccagta gttcattcac tttttgacga
    27661 cgttcctgtt tcgattttcc agaatacttt aaagaaaaat caacgttgtc aaaaatagtt
    27721 cggctttcca ttagattgaa atgctgaaag atcataccga tggcctttct ttcttttcgc
    27781 aattctttgg gagagagctg actcagattc gtctgattga caatgacttc tcctgctgtt
    27841 ggtttctgca gcaaattcat cacacgaact aacgtacttt tccctgcacc tgagtaaccg
    27901 acgattccat aaacatcgcc tttttcaatc gttaaatcta cagaatctac tgcctgtatc
    27961 tctttttctt tttgtttgaa acatacagaa acatctcgca attcaatcat taaaattcct
    28021 ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt
    28081 ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac aactcctctt caagtacgat
    28141 catactctag caagatatcg gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa
    28201 ttgctcgatg gccattttcc aatagtcgtt ccttgcttct tctcagctac cgaagctctc
    28261 tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa ataatattca aaaaaaaagt
    28321 ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgcggtac cacctttttt
    28381 cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt tcgttttaac
    28441 ggtcgaacag ttccgaattt gcctacttat cgacaaatta ttctccaagg ccattttcag
    28501 cttttcttac ttgactcttc tcaccagcca gagcctctct gcaaagtttc caaacttact
    28561 ttccttttca acgaaatcga tattttgttg ttgggattaa tctacatgag gaaaactgaa
    28621 aagtcaagta cgaaattcaa attatttttt ctatatttat ttttctgttt ttttatttaa
    28681 catttcaata acagttcgaa gatcttttcc ttccgcaaga agtttcaatt tttccttttc
    28741 ttcgacggta taatttcgga caaaactgga atcaggtatc aaaacaacgc ctaatcctgc
    28801 tcgtgaagca gctgttgctc ctactaaaga atcttccact gcaaaggctg aactagcttc
    28861 taatcctgtt cgttccaaag ctgtcagata aggaatcggt gaaggcttgt tctccgacac
    28921 ttcgtctccg aatgtaaacg tgtccaagta atcagctaat ttaaagtgat ccaagatatc
    28981 tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac gttcttttaa
    29041 gtctgccaat atttcttttg catatggttt taagtggatg gctccgcttg tcagctgttg
    29101 ataaaaatat tcttcacgct tcgctcggac ttcctgtact ttttcagggc tacctgctgc
    29161 tttcactaaa atatcaaaac tttgtttcca gttttttccg ccccaagcgt caacgatttc
    29221 ttttgggata tggatatcaa attgctgaaa tccccaaagc cagccatcac gatacgtttt
    29281 ttctgtgtca actaacaaac catctaaatc aaatattgca cctttcatct atacattaat
    29341 cctttccaaa acaatacaca tagtttagca cgactggtcc aaaaagaaca gaagcaaaga
    29401 accagccttc tcgtttataa gagttcttgg aatcgacgga tatagatttt cttcacagct
    29461 gtcacaagcg ccagataagc gatgactgta gcaaataacc ataaccagta attgccagga
    29521 agtggcatca agcctaattc agcaccaaaa cttgtaaaag gaaggacaga accgacaata
    29581 attcctaatg tagtgataac aaacatggca aatgttgcgt tgctttggat aaatggtact
    29641 ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc atagtgattc gacaaaccat
    29701 cctgcatgga ataatgcgat gaaaatcact cgctgtgaac tatccaatgt atggaaactt
    29761 ccgcccacta cagcaggaca aataacaaaa tacatcaaca agtaagtagt aatatcaaat
    29821 atcgaacttg ttggaccaaa gtaaaccatg aattccccaa tactagatgc attccatttt
    29881 tttggctcgt ccaaatattc tttatccatc cgatcccatg gcaaggaaat gcaggaaaca
    29941 tcatagatca gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac
    30001 gtacttgcaa tcagcacgga aaacatattg ccaaaattag aacttgctgt agccttgata
    30061 tatttcatga tattcccaaa agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc
    30121 aaatcttttt ctaaaaggat cacgtctgca gattctttcg caatatccac agctgtatct
    30181 acagaaatac cgatatcagc cactttcatt gcaggagcgt cattgatacc atctcctaaa
    30241 aatccgacag tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga
    30301 ttcaatttta cgaatacttc atggtcttca gctacttgtg ctaattcatg atcactcatt
    30361 tccgagattt tctcaccggt aaccaattca ttgatctcta gacctacttg acggcacact
    30421 gaacgtgtaa ctaattcgtt gtccccagtc agaactttta cagctacacc atgttctttc
    30481 agagctttca acgcttgttt cgttgtttct tttggtggat caaggaaagc aagataacca
    30541 atcaaaacca tgtctgactc atctttgact gaaaattctc caacgacgct tggattggtc
    30601 ttttgtgcga caccaatcac tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt
    30661 acggcttctt ttcgttcttt tgttaatggt gtaacagtac cgccaagatc aataaattta
    30721 gaaatgttca gcatttcttc aatcgcacct tttgtgatca tttgtgtttt accagctgga
    30781 tcagcaacca caacactcat acgacgacgt tcaaaatcaa aaggaatttc atccactttt
    30841 tgataattga tcttatctgt atctaaagtt tctgttgctt catcaatgat agccacatct
    30901 aacaaatttt tcagccctgt ttgatagtag ctatttagat acgcatggcg caaaacacga
    30961 tcatcttcat ttccttcaca atccatatga tattcaagaa tgattttatc ttgtgtcaat
    31021 gtccccgttt tatccgtaca taacacatcg attgcaccaa agttctggat ggcattcaaa
    31081 tttttgatca ctgttccttt tttggccatc acacttgctc ctttgaccaa attcgtcgta
    31141 acgatcattg gaagcatttc aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa
    31201 gcttctagcc aatccccttt tgtaagccca ttgatgacga taactgctgg agccatcaat
    31261 gacataaatt tgatcagtag catggatgtt ttatggatgc ctaattcaaa gcttgattga
    31321 attggttttt cagatagcgt ttctgctaca tgaccaaata gtgtttcatt tccaacagaa
    31381 acaatgattc cttccgcact tccactgatg acattacttc ccataaagac aagattttcg
    31441 taacttgttt cgctttgctg atcgtcttca catactttcg catgcttttc tactggatag
    31501 ctttcccctg tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa taaacgcata
    31561 tccgctggta tcatatcccc tgctgaaagc cgaacaagat ctccacagac gatttcttcc
    31621 ataggaatct ctgtgtactt tccattacgt tttacagctg ccgttacttt cacaagtgat
    31681 ttcagttttt cagccgcttg attggaacgt acggactgaa tcaacgtcat tgtcccgctg
    31741 acgataacca tagtgaagat aatggctaca ccgaaaagat ctttatctcc aggagctgct
    31801 aatacatatt ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa tgtaaatggc
    31861 gtgatgtatg ctttcaatat ttctacaatg aatggtgttt ttttgccata tgaaatcgca
    31921 ttttcaccgt aattttctct tgaacgttcg atttgttctt ctgtcaatcc atttgctgat
    31981 gcatggaata gttcaaataa ttcttgtttc gtttttccag caaaagctgc atagattttt
    32041 tgtgtatctt tctttttttc catctcaatc tctcctttgt gtccaaaaga cagctgttct
    32101 cgctgctttt ggtctttagc tattcatcgg atcgatagag aaaaaactga aaggggctga
    32161 gaacagatag ataatagccc tggcctttcc tctagatcct tgtttttcga atgactgtca
    32221 tccatttgtt ctcatctcct ttactaagta attttaattc atgacttcca atcatttttc
    32281 gaatagttat ttttccaatt agaccacctc ctttttattt ttcaccgatg gggatttgcg
    32341 gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa
    32401 agcttctcag atctcatcgt tgagttttga cactgtacaa cgtaaaaagc agatacctgc
    32461 ttttagctac cttatgaaaa gccttatctc gacaattcct gttctaccca ttggcgtctc
    32521 tcgacgtttt tgggcagtag cctatgtttg tataggagcc tcacctaaca aatcttattc
    32581 ggttttcctt cgttaatata acaactaaaa aagaggatgt caaatcaatt ttccatgttt
    32641 ttcatacttt ttttaaaata cacacgttat attcctgtct cacgcggttt tatttgtctc
    32701 attttccacc tcccgctata atagaaatat agtaggaaac gaggtgctgt tatggaaaga
    32761 acacttgtta ttatcaagcc agacggcgtg agacgccatt tagtcggaag catcatccaa
    32821 cgttttgaag caaagggact ggccattgct gaaatgaagt ttgaaactat gactcctgaa
    32881 ttagcaaaag aacattatca gcacttgact gagcgcagtt tttttgatga actgatcgat
    32941 tacatgactt ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc
    33001 gtccgtaaga tggttggagc aacaaaagct gccgatgctg ttccaggtac gatccgagga
    33061 gactacgcgc tgccggggac tgaaaacatc attcacgctt ctgattcccg tgatgctgcg
    33121 gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg
    33181 gcgaagtgat acaaattcta aactgctggt ctagttgctg ggttcagcaa tggccagcag
    33241 ttttttctct aaggacctcc gttgattctt tcctaacata tattcgagca ctgtgaagaa
    33301 ttcttggtag tctttcgtct tttcttcccc taaagcagca gaaatcaatc tttgataata
    33361 atctgccatc aatgtttcat agatcactgt acttctttct ttatgtgcta agctccgttt
    33421 catatcttca ttcacactac gtgccttttt caaccgacca ttttctatat aaaacgaagt
    33481 caggttcatt aaaaaagtaa agagatactg ctcatgattg ttcaactgtg ctaatcgtcg
    33541 cattcttttt ttgattttca aataattgtt ctcaatgaac cgatcactga atatccaagg
    33601 aaaatcagct agaatagata gttcaaattg accatagtct ggtatctgca tcaagtattc
    33661 ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct gtctcagata cttctgtttt
    33721 ttcgaccatg tgaagattca aaaccaaata atacagatct tctgttttct taaaatgcct
    33781 acggttattg ctgatctctt tataaggatc aaagctgtct tcttgtaccc gatctagcat
    33841 tcgtcttttt ttctttaact ttattggttc atccagctgg tttcgcatcc attcatattc
    33901 tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat gttacaaatg acattttttt
    33961 cactgaattt gtctcaatcc cgactagaga agtcttaaaa cagattcctt ccgctaattg
    34021 ggactgtgtc aacttcttgt tgactcgaag tttcttgatc aattcaccat catacataat
    34081 agttctcctt tttaatttaa atttttccac attcaataaa tagatacgtt aaaaaggaaa
    34141 aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa ataccaacct tcaagcatta
    34201 gaatgttgtc taactactga aggttggttt gattgtttat tgattatttt tttgctgttc
    34261 aacgtctgtt cttgttactg gatataagtt ggttgttgtt ttattaccat tttttgaaaa
    34321 gacacttgtc gaatgttcgc ctttttgctt ttcgattgct tcattacgtt ccgcttcatt
    34381 tttatagtca tattttttcg gatcaatcgt ctcaaatccg tttggcacat aaaaccgcaa
    34441 gagattttcc tgattcagct tatctgaaag acttaacgca gtcttgactt tttcctgatc
    34501 tttggagatt tcctcatcga ttcctgctgc tttcgtatcg atgatttctc ctgtctgatt
    34561 attgtaagct tttcctccta ggacggtgta tttgggtgtc acaaagttcc catttcgaaa
    34621 agcaactact tgatcatgct gagaagataa taagtctgta ccaaaatgaa gataatcttt
    34681 atcatcgatt cccaatagat gtagtaatgt cggcaaaaca tcgatttctc caccgtattc
    34741 atgattgact gtccctttag tataaccagg catatggatc atcaatggca ctcgttgcat
    34801 ctgtgcgttg tcaaattcat cccatgtatc tggatcttta ccaagagcgc ttgctaaatc
    34861 tttattatct gtattcgaga tcccaaaatg atccccgtaa atcacaaaga ttgaattttg
    34921 gtaaatacca gattttttca aatacgtaaa aaattgttcc aatgattgat ccagataatg
    34981 tgccgttcga acataattat ctactgtgct attgccagta ttcaaagatg gaaagtcgaa
    35041 attcttgtca tccgtataat aaggcgtgtg gttggttacg gaaagaaatt tcgtgtaaaa
    35101 cggctgttgt agatgctcaa gatattgggc agattctctg aataaatctt tatctaatat
    35161 cccataacct aacgtttcgt cggattcatc aaaataagat cgatcaaaga aattgtcata
    35221 acctaagttt ttatatacat gatctctatt ccaaaaacta gctacattcc catggaaaac
    35281 agcgcttgta tagccttgct tttgtccaag gatcgctggt gcggcttgaa agacattgtc
    35341 tgacccaagt tctgtgaata atgagccttg aggtaaacca aatgtgccag tttccaacat
    35401 attttcagca tcacttgtct ttccctgtcc tacttcatga aagaaattgt caaagctgat
    35461 cgttgcttga ttctggaaaa tactatttaa aaacggtgtg acttcttgtc cgtctacctt
    35521 catattaatc aaaaactgct ggaaactttc taaatggaga acgatcacat ttttcccttt
    35581 agcgatccca aatgtttcgg gatttggctc tgcataatgt ttctttgtat aatctaaaac
    35641 ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat
    35701 tccgtcataa atagtatacg catcgattcc tagatatttg acaatatatg aacggtcaaa
    35761 tgttcgctgc agcaattgtg gacggttggc ttcgctcagc gctagatttc ctgaaaaaat
    35821 aagccctgaa agacaggtaa cagcgatagc catcggtttg ccgacagggt tgcttttgat
    35881 aggtattttc ttgaccttca accaaaccag tagcccaatg aacacagcga tatccagcca
    35941 gtagatgaca tcatggggct tcatcaaaga aaaggagctt cctgacaatc cttgcgacac
    36001 tttcgaaaag cctaatacag atttgactgt aatgaaatct gtgaattccc gataaaaaat
    36061 aatattcaag tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa tagctaacgc
    36121 tggctttggc tttttaatat acaaagaaag gccaaaaaac aataaggtag tcgcaattgg
    36181 gttgatccac aagataaagt actggatcgt accttccaca cctaatgaaa aatctgcata
    36241 gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg
    36301 attttggcac aattttttaa gttgtgtcac gtatatcact tctctctatt cacatttttt
    36361 cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta agattttttt
    36421 attctttcta aagattattc cgtccacttt tcacagcata aaaccgctgc gatgtaagcg
    36481 attaaactaa tgaagagata aaagtcttgt ggtgaccgct tcactccacg gtattcaaaa
    36541 gccagtttct cggtattcgt acaaaaaaag aggctgtgac aaaattcttg tcgcagctcc
    36601 ttatttcttg atgcaggaca gctttttcac aacttcttcc ttaaacggtg ttctcctgcc
    36661 aactcctcgg ccacaagtca caatatttat aaaacatgag aagctgtttt ttgaaattgt
    36721 tccttgtggc tcagagttaa acggcaggct caagacctct tgatacacgg tgttcaatca
    36781 gctgaccctc ggtattagat tatgtcagga cgctaatttt acaacctctt ggtaaacggt
    36841 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatata aggagctatt
    36901 ttttgaaatt gctccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac
    36961 ggtgttcaat cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
    37021 tttgccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctgtttttc
    37081 attgtccata ggattgatat tgtccttgtg atgactcatt cgtaataatt gaggcttgat
    37141 tttcttcact tcccgttatc gtttgtccac tgccagaatc tgaaggactt gttcctgtcg
    37201 actcgttttt ttgagaagag acagttgttt tcttgacttg ttcatttgtc tttttccatt
    37261 ccgctaaacg atcggcgacc acttttttgt ctactttttc agaataagcg gtgatcactt
    37321 ttccattttt caaacgggct actttgtcgc catcatgagt caaaagcaaa acggtcaaat
    37381 gcttgtcttt aaattgatat tgtctgagag aaatcgcact aagtacgtca tcgatattaa
    37441 taaattgatt ttcttctttt ccttttggca aataaatgtc caattcgcct ttttcgtaat
    37501 tggcagttat tttatactcc gcccaccaat aagttgagaa ttcttttttg aaattatcta
    37561 ctgattcttt gcttgctacg attttttctg tttctttctg agcaagatct gctgtcattt
    37621 ttcctgaaaa tgctgcggat aaaataagca gtaagaaaaa accgaataac caaaagagaa
    37681 gaaacggtct ttttttcctc atgctttcct ccactcctat ttaattttga ctattctatt
    37741 atatacaaaa aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa taattgaact
    37801 tctttttcac aaaatggtaa gaatggagaa tttcacgcat ttgctctcaa ctattctttc
    37861 ttaagcctca aatccgtttt tatctgatac gtctttgaac caatgacccg attttttgat
    37921 tgttcttttt ccatcttgtg ctaagtctac tgaaataaag ccatatcgat tcttatatgc
    37981 attggtccac gaccagttat ccatacatgt ccacatatga tagcctaaac aattggcacc
    38041 ttcttgaatg gcttgatgga catatttcag atgagattgg acaaattcga tccgataatc
    38101 atcgtggatc atgccatctt ctttgataaa tcgtgcttcg ttttctacac ccataccgtt
    38161 ttcagaaata aagcagtcga tatttccata atgatctcgt acattgatca aaatatcata
    38221 gattccttgt tcatagatct cccatccgcg ataaggattc attttacgac caggcatttc
    38281 ataattgtcg aaataatcct caggcattgg accgttttta gtatcgattg gtgtttcttt
    38341 tgcttttacc cggcgtggct ggtaataatt gatacctaaa agatctactg tattttcttt
    38401 gataaccgtc aaatcctctg catggatcac aggcatgaga tcaagttctt ttactatctc
    38461 aatcaattca cttggaaact cgccttttac agctggatca aggaatgaac gattgaagaa
    38521 cgcatctgct atttgcgctg ctttaacatc ttcagggttt gattcgtctc ttggatagct
    38581 tggtgtcaga ttcaagatga taccaataac accaccttgc tccatttcat ggtaagattt
    38641 gatggctaac gctgaagcca acgcttcatg atagccaact tggatcgctt ttttcatatc
    38701 tgactcttct ggataatgcc atcgatataa ataacctgct tcgactggaa cgattggttc
    38761 attatgagta aaccattttt ttactttcga accaaacaat tcaaaacaac ttttggcata
    38821 tttttcatag gcttcaaccg tttctcggtt caaccatccg cctttttctt gcagatacat
    38881 cggcatatcg aaatgataca gattcacaaa gggttcgata ccgttttcaa tcaactcatc
    38941 aaaataacga tgatagaaac gaacggcttc ttcgttgatt gttccagatt catcaggtat
    39001 caaacggctc cattggatag atgttcgaaa tgaattatgc ccagtctgtt tcatcaactg
    39061 tacatcctct tgatacttcg tataaacttg cgaggttttt tcaggtccta cttgatcaaa
    39121 gaacttttgc ggttcttttt tgtaccaata atcaaaaatg ttctctcctt ttccatcgcc
    39181 tgaaatcctt ccttctgttt gtgggccact tgctgcagaa ccccaccaaa aatttgctgg
    39241 aaattgatat tttttcatca aagcacctct ttttcttttt tagttcgttt tagttgattc
    39301 catgtatgat attttccatc acaatcactt tttcattttc ttcctcttct tttttgattc
    39361 gttttaacag ctgttctgct gctcgttccc cgattttatc tgttggtggc gtgatcgtga
    39421 ttttcggaag gatactcaga tcgtttttat ctaaataatc atagtgactg aacgcaatat
    39481 ccttcccgat tctgacacct tcttgattga aacattcgat ggcaccaatc gacatattgt
    39541 aattgctgac aaaaatagca gttgcccctt tttctaataa tgcttttgcc ccgtaatagc
    39601 cgctttttct agaatagtct cctgtaaaga cctctaattg gatcgatgga tcattttgga
    39661 ccgcttcttt ggctcctgct aaacgttctc ttgctacgta gtctgtttga gtggcagcaa
    39721 taaatccgat ttttttatgt ccttgttcac catgatgagc gatcactttt gctacacttt
    39781 ctcgatcatt cactaatatt gtatcaatac ctgctggtcc ttctggacaa ttcaatgcga
    39841 taactggaat actcattttt gtgagcattt caaatccaga ccactcttct tcggataaga
    39901 agacaatcag cccatcgatc acatgcttca tcaggtattc aattttttgt tcaatttttt
    39961 ctggctgatc ggcaaacccg cttaataata agctataccc attttcttca gctactcttt
    40021 cgatccctga gacaacttcc gacccaaaac ggctggaaat cccgtttatc aaaagtccaa
    40081 tagagaagct tcgattattc ttcaaccctt tagcaatgat gttttcttta tagtccaacg
    40141 ctgaaatcgc ttctgctatt tttttcatgt tcttttcctt cagttgctga ccattcaagt
    40201 aacgggaaac tgttcccact gaaacaccag ccatcttagc cacatcacgg atcgatgcca
    40261 tacattctca ctcctgccag ttacatttga tttctctttc ttgttgttcg attatgatga
    40321 ccgcattctt tgcatagtgt tgcttgtcat caatatccat ataactaaag tcttcagtag
    40381 agcaacttcc tctcactatt aattatactt aaacaagtat ttgaaccggt tcatttttaa
    40441 tatagcattt ttttaaaaga ttgcaacact tttttaaaac gctttctgaa agtggcggat
    40501 tttttgcctt tttaaaattt tattctaaaa tttactctat ttgtgggtgt tttgtgcgtt
    40561 tttctcttcc atccgtttga agttttacat tccaatggta taatttcttt atcctatcaa
    40621 atgggggaag tattttgaag aaattaatcg tactatttat tttgtctgcg ggacttttga
    40681 cattgactgg ctgtgatttg tttggcggct ctgaccaatc ctcggacgaa acaaccgatt
    40741 cttctgtctt aaaagcggaa aaagcagata ccaccaacag tgtatatacg ctaaaagtac
    40801 gtaaagatat ggatttatcc gcgggacagt tgacatttac agataaaaaa atggaatgga
    40861 cacgcacgta tcagacacaa ggaactgatt cttctgaatc atctgaagaa aaaacagtgc
    40921 tgacagatat caagatcact accaaagatg acgaatatat tatttcagga aaagaaaata
    40981 aaaaagaaac aacagtcata tttacaaaaa tcggtaatta ccgtttgaag gatgaggatg
    41041 gcaacatcta cagtctatag tgccgtctgt tggatcttct tttgtttctc cccttctttt
    41101 ctgctatagt aaagaagcaa gatgattgta aaggagacga aatggatgat cgatagtcct
    41161 gattattgta catgtaataa caacaaaaat atccatacag aatttaatga ccaaggaagc
    41221 ttgagcatat gcgacaactg tcacaaacta gtcaaagatt catttaaaac atttgaatct
    41281 tatcctgttc aaaattatgt aaaaagtaga agaagataaa caaatccaag caaaactcac
    41341 tatggctgat ttttatgaac ttcaaactca gcttcttcct ttgatcaagc ttcatcagta
    41401 aaacaaacag tggatcattt cagtttacaa atcaaagagt aaagatttat ttatttcaaa
    41461 tatttgaata taaaataacc aaacgataga aaggcttatt ctacatcacg tagta
//
LOCUS       DANKMN010000016        40815 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000016 DANKMN010000000
VERSION     DANKMN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            23..646
                     /locus_tag="PW224_000849"
     CDS             23..646
                     /locus_tag="PW224_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907557.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="PW224_000850"
     CDS             1096..2145
                     /locus_tag="PW224_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2907558.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="PW224_000851"
     CDS             2142..3023
                     /locus_tag="PW224_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907559.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="PW224_000852"
     CDS             3026..3796
                     /locus_tag="PW224_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2907560.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="PW224_000853"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="PW224_000853"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL2907561.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="PW224_000854"
     CDS             5107..6270
                     /locus_tag="PW224_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2907562.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="PW224_000855"
     CDS             6387..6797
                     /locus_tag="PW224_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2907563.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="PW224_000856"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="PW224_000856"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2907564.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="PW224_000857"
     CDS             9370..9654
                     /locus_tag="PW224_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907565.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="PW224_000858"
     CDS             9814..10050
                     /locus_tag="PW224_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2907566.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="PW224_000859"
     CDS             10047..10445
                     /locus_tag="PW224_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2907567.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="PW224_000860"
     CDS             complement(11112..12056)
                     /locus_tag="PW224_000860"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2907568.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="PW224_000861"
     CDS             complement(12352..13296)
                     /locus_tag="PW224_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2907569.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="PW224_000862"
     CDS             13517..14731
                     /locus_tag="PW224_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2907570.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="PW224_000863"
     CDS             14753..16768
                     /locus_tag="PW224_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL2907571.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="PW224_000864"
     CDS             complement(16851..18224)
                     /locus_tag="PW224_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2907572.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="PW224_000865"
     CDS             complement(18250..18534)
                     /locus_tag="PW224_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2907573.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="PW224_000866"
     CDS             complement(18536..20551)
                     /locus_tag="PW224_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2907574.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="PW224_000867"
     CDS             complement(20730..21035)
                     /locus_tag="PW224_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL2907575.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="PW224_000868"
     CDS             complement(21124..22155)
                     /locus_tag="PW224_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2907576.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="PW224_000869"
     CDS             22362..23237
                     /locus_tag="PW224_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2907577.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="PW224_000870"
     CDS             complement(23389..24231)
                     /locus_tag="PW224_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2907578.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="PW224_000871"
     CDS             complement(24323..26200)
                     /locus_tag="PW224_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2907579.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="PW224_000872"
     CDS             complement(26197..27618)
                     /locus_tag="PW224_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2907580.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="PW224_000873"
     CDS             complement(27621..31010)
                     /locus_tag="PW224_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2907581.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="PW224_000874"
     CDS             31267..32658
                     /locus_tag="PW224_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2907582.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="PW224_000875"
     CDS             complement(32731..33378)
                     /locus_tag="PW224_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2907583.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="PW224_000876"
     CDS             complement(33386..34072)
                     /locus_tag="PW224_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907584.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="PW224_000877"
     CDS             complement(34078..35151)
                     /locus_tag="PW224_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907585.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="PW224_000878"
     CDS             35373..36047
                     /locus_tag="PW224_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2907586.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="PW224_000879"
     CDS             36052..37392
                     /locus_tag="PW224_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2907587.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="PW224_000880"
     CDS             complement(37541..38527)
                     /locus_tag="PW224_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL2907588.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39251..40282
                     /locus_tag="PW224_000881"
     CDS             39251..40282
                     /locus_tag="PW224_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2907589.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40391..>40815
                     /locus_tag="PW224_000882"
     CDS             40391..>40815
                     /locus_tag="PW224_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2907590.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT        12670 a         7414 c         7660 g        13071 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga attaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    38641 tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata aaatgtcatt
    38701 tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg tgatggttta
    38761 atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat tctcaatata
    38821 attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac tatttctaat
    38881 acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa taaatggaga
    38941 gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa gagcctcctg
    39001 ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag ctaaaaagag
    39061 gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta aaaatgtatc
    39121 ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta agatcaagac
    39181 aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac actattggag
    39241 ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa agcaacgtac
    39301 acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt ccctttcatt
    39361 aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt tgagaaaagt
    39421 attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag attatcagaa
    39481 tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga accaagaaac
    39541 ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt cattaaatca
    39601 ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc ttttgggtac
    39661 aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac ttttcaaaga
    39721 ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag ctagctttga
    39781 agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt gcggattaaa
    39841 gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc agattcttaa
    39901 tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag cgatgccatt
    39961 tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt ttgtttttcg
    40021 ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga gcaaaattac
    40081 agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt atgcagaact
    40141 atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc tctcatccca
    40201 ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat tagcggcatt
    40261 cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat aaactatgtt
    40321 attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa caaaagataa
    40381 tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta cgaattttca
    40441 agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac tttcagcttt
    40501 cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc
    40561 ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt ataagagttc
    40621 cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat
    40681 gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt tagaagaccc
    40741 tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa
    40801 atcaaaacag attcc
//
LOCUS       DANKMN010000017        39055 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000017 DANKMN010000000
VERSION     DANKMN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(324..1106)
                     /locus_tag="PW224_000883"
     CDS             complement(324..1106)
                     /locus_tag="PW224_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907591.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1171..2007)
                     /locus_tag="PW224_000884"
     CDS             complement(1171..2007)
                     /locus_tag="PW224_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2907592.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2025..3434)
                     /locus_tag="PW224_000885"
     CDS             complement(2025..3434)
                     /locus_tag="PW224_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2907593.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3448..5271)
                     /locus_tag="PW224_000886"
     CDS             complement(3448..5271)
                     /locus_tag="PW224_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2907594.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6114..6440)
                     /locus_tag="PW224_000887"
     CDS             complement(6114..6440)
                     /locus_tag="PW224_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907595.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6486..6848)
                     /locus_tag="PW224_000888"
     CDS             complement(6486..6848)
                     /locus_tag="PW224_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDL2907596.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6885..7099)
                     /locus_tag="PW224_000889"
                     /pseudo
     CDS             complement(6885..7099)
                     /locus_tag="PW224_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7227..7562
                     /locus_tag="PW224_000890"
     CDS             7227..7562
                     /locus_tag="PW224_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2907597.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7638..9635)
                     /locus_tag="PW224_000891"
     CDS             complement(7638..9635)
                     /locus_tag="PW224_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907598.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9733..10008)
                     /locus_tag="PW224_000892"
     CDS             complement(9733..10008)
                     /locus_tag="PW224_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2907599.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10240..10974
                     /locus_tag="PW224_000893"
     CDS             10240..10974
                     /locus_tag="PW224_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2907600.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11013..12155
                     /locus_tag="PW224_000894"
     CDS             11013..12155
                     /locus_tag="PW224_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2907601.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12250..13641)
                     /gene="sufB"
                     /locus_tag="PW224_000895"
     CDS             complement(12250..13641)
                     /gene="sufB"
                     /locus_tag="PW224_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL2907602.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13646..14116)
                     /locus_tag="PW224_000896"
     CDS             complement(13646..14116)
                     /locus_tag="PW224_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL2907603.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14103..15338)
                     /locus_tag="PW224_000897"
     CDS             complement(14103..15338)
                     /locus_tag="PW224_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2907604.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15335..16621)
                     /gene="sufD"
                     /locus_tag="PW224_000898"
     CDS             complement(15335..16621)
                     /gene="sufD"
                     /locus_tag="PW224_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL2907605.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16634..17407)
                     /gene="sufC"
                     /locus_tag="PW224_000899"
     CDS             complement(16634..17407)
                     /gene="sufC"
                     /locus_tag="PW224_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL2907606.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17604..18452)
                     /locus_tag="PW224_000900"
     CDS             complement(17604..18452)
                     /locus_tag="PW224_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2907607.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18476..19162)
                     /locus_tag="PW224_000901"
     CDS             complement(18476..19162)
                     /locus_tag="PW224_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907608.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19155..20189)
                     /locus_tag="PW224_000902"
     CDS             complement(19155..20189)
                     /locus_tag="PW224_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2907609.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20252..20508)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20584..20922)
                     /locus_tag="PW224_000903"
     CDS             complement(20584..20922)
                     /locus_tag="PW224_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL2907610.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20924..21280)
                     /locus_tag="PW224_000904"
     CDS             complement(20924..21280)
                     /locus_tag="PW224_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL2907611.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21286..22473)
                     /locus_tag="PW224_000905"
     CDS             complement(21286..22473)
                     /locus_tag="PW224_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2907612.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22664..23509)
                     /locus_tag="PW224_000906"
     CDS             complement(22664..23509)
                     /locus_tag="PW224_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2907613.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23743..24708
                     /locus_tag="PW224_000907"
     CDS             23743..24708
                     /locus_tag="PW224_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL2907614.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25085..26128
                     /locus_tag="PW224_000908"
     CDS             25085..26128
                     /locus_tag="PW224_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL2907615.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26190..26654)
                     /locus_tag="PW224_000909"
     CDS             complement(26190..26654)
                     /locus_tag="PW224_000909"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL2907616.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            26900..27847
                     /locus_tag="PW224_000910"
     CDS             26900..27847
                     /locus_tag="PW224_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2907617.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28058..30130)
                     /locus_tag="PW224_000911"
     CDS             complement(28058..30130)
                     /locus_tag="PW224_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2907618.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30063..30440)
                     /locus_tag="PW224_000912"
     CDS             complement(30063..30440)
                     /locus_tag="PW224_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL2907619.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30569..31147)
                     /locus_tag="PW224_000913"
     CDS             complement(30569..31147)
                     /locus_tag="PW224_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2907620.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31279..32571
                     /locus_tag="PW224_000914"
     CDS             31279..32571
                     /locus_tag="PW224_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2907621.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32631..33749)
                     /locus_tag="PW224_000915"
     CDS             complement(32631..33749)
                     /locus_tag="PW224_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL2907622.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33763..35184)
                     /gene="arcD"
                     /locus_tag="PW224_000916"
     CDS             complement(33763..35184)
                     /gene="arcD"
                     /locus_tag="PW224_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL2907623.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35430..36551)
                     /gene="mnmA"
                     /locus_tag="PW224_000917"
     CDS             complement(35430..36551)
                     /gene="mnmA"
                     /locus_tag="PW224_000917"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL2907624.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            36955..37554
                     /locus_tag="PW224_000918"
     CDS             36955..37554
                     /locus_tag="PW224_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL2907625.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37692..38978)
                     /gene="efmA"
                     /locus_tag="PW224_000919"
     CDS             complement(37692..38978)
                     /gene="efmA"
                     /locus_tag="PW224_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL2907626.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
BASE COUNT        11264 a         8220 c         6832 g        12739 t
ORIGIN      
        1 gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa
       61 aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat
      121 cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt
      181 catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag
      241 cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat
      301 aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca
      361 gttacctgtc cgttcttaaa taccacagaa taatcttttt tcccattttc aagtaaccga
      421 agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct
      481 aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt
      541 cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat
      601 gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat
      661 gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg
      721 gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt
      781 cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat
      841 ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct
      901 acataataat cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct
      961 gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc
     1021 aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc
     1081 tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt
     1141 tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag
     1201 ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat
     1261 tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc
     1321 atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt
     1381 cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc
     1441 tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc
     1501 ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa
     1561 ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt
     1621 tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact
     1681 gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt
     1741 aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc
     1801 cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa
     1861 tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc
     1921 aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact
     1981 gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt
     2041 cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta
     2101 agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg
     2161 gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt
     2221 aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta
     2281 attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata
     2341 ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact
     2401 acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct
     2461 tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg
     2521 ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag
     2581 gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag
     2641 ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc
     2701 aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata
     2761 gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga
     2821 attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa
     2881 ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc
     2941 tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca
     3001 tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa
     3061 taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca
     3121 tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc
     3181 ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc
     3241 tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct
     3301 tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc
     3361 tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc
     3421 tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt
     3481 taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat
     3541 cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg
     3601 tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt
     3661 cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat
     3721 cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt
     3781 ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt
     3841 cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc
     3901 tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat
     3961 aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt
     4021 ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa
     4081 accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg
     4141 ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt
     4201 gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc
     4261 aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc
     4321 agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt
     4381 aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc
     4441 gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat
     4501 gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt
     4561 aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt
     4621 aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa
     4681 agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc
     4741 aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag
     4801 tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact
     4861 actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc
     4921 acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga
     4981 aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt
     5041 ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc
     5101 gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct
     5161 tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc
     5221 ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc
     5281 tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag
     5341 tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc
     5401 caataatagc acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt
     5461 tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact
     5521 tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc
     5581 agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt
     5641 caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag
     5701 cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca
     5761 atagccaatt tcaatcattc cgagatataa aggtatcgat agaatgattt gggctagtat
     5821 tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca tgtagtaagt
     5881 atttcaattc caccaattat caataaaaat accaaacata tccataataa ttttttcttt
     5941 ttcatttctt ttatattgtt ataacagtga ataacataaa aattcccgac aatcattgta
     6001 atattttttt gtcacctgta ttagtattta atttatagga aactataaaa actccctagt
     6061 gaatttacac ctttgggagt tcctgtttgg acggtatgca taatacattt aaatcaattt
     6121 aacattaaca cctcagcata tggtatctcc acatatatac ggtctttctt tggatagtac
     6181 tcaatattta tgttgatagc acttccattg tctaatatag ctttagcccg gtatgtgtta
     6241 tctccatttt catgagtaat cgtcacatca tcacatgatc gatttaagtt gtactgttct
     6301 tgtaaaatct gtgttaccaa tggtttcgat gacttctcta atccagatgt acctgtcatt
     6361 tttgaaaaaa tgatacctat aatagccaag acaacgataa ctataatgat aaacttcttg
     6421 ttcatattta ctttattcaa ctttttatct cctatctcca aaataatttg ctcttatccc
     6481 tcttatcagt acattgacat ccctaaaagt acaccaaaga ttgctccagc tacccctgct
     6541 atcatcaaaa tcgtacccat ttgttcatct ttatctcgat ataagtagcc cataataaca
     6601 gcaacagcac caaatacaac aggaataaat gctaacgcta ctaccataga aatccaacca
     6661 ataatcaaat acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta
     6721 attgtctgct cagtactttt ctttactgcg tctgctccag ttgtctcatc tgtttcttgt
     6781 agtgctccgc aaaactggca atacttactt tcatctgtta tttctttgcc acattttcta
     6841 caatacatgt cttctcctcc taaaaacatt caattgttta ttagttaact gtttacttta
     6901 ttaaatacat cagaaaacag ctcattcatt ggtacactta atatctcaga aagcatcttc
     6961 atttgatagg gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta
     7021 acattcattt ttttgctaac tccgtttgag aaagtccttt ttcttttctg tatcggctga
     7081 gattacttac ttttttcatg attctcccct cctgttttat tatatacaac aatatgatgt
     7141 atttcaactc ataaaatata aaaaaacttc aaataccatt acaaatacaa caacttgttg
     7201 tactatactg agatgggggt gtattaatga aacagaaaca atatgcaaag gtcattagag
     7261 aaaagcgaaa agctctcggg tggacacaaa aagaattagc tgaaaaaatc ttctctacac
     7321 aacaggcagt agcaagatgg gaaaattctg taacagagcc taacctcgat agcttaaccg
     7381 ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt gtcgtagatt
     7441 atgaggaaga gtttttggct ctctatcgtt ctcttagcac tgaagatgca gttcgaacaa
     7501 ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt
     7561 gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa taagattaag
     7621 agctatggat aacctgtcta gtttaataca attgggtctt ttctcttcct tactctccgt
     7681 ccatcatctc ttgttacact tgtaaattga taaagaggaa ctaattcaac tcctgataat
     7741 atcaattctt ttattacttc atctatccct gctccttttg ctaggtagac taaaaaacta
     7801 ctacttgctt tcacattgta tagtctctta atatccttag aagctcgttc aagagtgaat
     7861 ccttgtttaa caaagtagcc tctgttttct tttaaagcat tttccacgct gttattccca
     7921 acattacgta gacgttcaca aacagtgtta gtaagttctt tctctgtcat tagatatgtt
     7981 ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc
     8041 ttgctaagtt ctgatcggga attttgagga aatactcttt tagcctcttt ttcgttaaaa
     8101 catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaaattt ttccagaagt
     8161 ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac gtctaatcga
     8221 tagacattgc ttctcttatg attaaagttt cctaacgctt tctcttcctt taacctattt
     8281 aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat caatcctata
     8341 aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt accatagtaa
     8401 aaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact ttccttacta
     8461 tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat ttcctctaga
     8521 taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc aaaaagtact
     8581 tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt ctctaatagc
     8641 caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac tccaataaaa
     8701 ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc agttttactg
     8761 gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa gtttttactt
     8821 gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc attaaaagac
     8881 atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc tttgtcgtca
     8941 ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct ccctgaatca
     9001 ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt aatatctaac
     9061 atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa aaacattcta
     9121 ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta ttcgtaaaca
     9181 gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat cttgtatttt
     9241 tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt atgaactaaa
     9301 ttgatagata aaaccttttc tatgcttaag tatctactat catctttact caaatctact
     9361 accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac tctggtttcc
     9421 acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa tgattgtgat
     9481 gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc taaacaccta
     9541 tctcttacat cagttttttc tttttttgta actggcttct ctaataattt ttctttatct
     9601 acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt ataaaattct
     9661 gttaaagact aaaacataca gataataaaa acaaactctt tattactata tgttttagtc
     9721 ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag attttttaaa
     9781 tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat
     9841 cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat actctagagt
     9901 gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg
     9961 ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc
    10021 tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa
    10081 aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac
    10141 acataatacc tcgataaaaa agaggtgtca atatctttgt aaaatttatt tacaacaccc
    10201 ctttttcgag gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata
    10261 aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa
    10321 aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaaa
    10381 actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt
    10441 gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt
    10501 ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa
    10561 aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg
    10621 ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt
    10681 aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc
    10741 atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa
    10801 gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat
    10861 cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca
    10921 tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa
    10981 gtactccttg taaactaaga ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga
    11041 aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg
    11101 aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg
    11161 acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg
    11221 aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg
    11281 caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat
    11341 cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa
    11401 gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat
    11461 atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa
    11521 atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg
    11581 gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg
    11641 aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata
    11701 aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt
    11761 ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac
    11821 agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag caacatgttt
    11881 ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata
    11941 ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta
    12001 gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa
    12061 atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag
    12121 gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt
    12181 tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt tctagctcct
    12241 ataacaggtt tacccaacac tgccttccat ctcataactg atcaatcgat tgagttcgac
    12301 tgcatattcc attggaagtt ctttggtgaa tggttcaaca aagcccatga cgatcatttc
    12361 agttgcttca gattcggtca ggcctcggct catcagatag tagagttgtt cttctgagat
    12421 ttttgagact ttggcttcgt gttctaaaga gacttggctg ttgtggatct cattgaatgg
    12481 gatcgtatcg gatttcgacc attcgtccat gatgatcgtg tcacattcga tatgagagat
    12541 cgaacccgca ctgtttttgg caaaggttac ttgtcctcga tagtttactt ctccgccgtc
    12601 ttttgaaatt gatttagaga cgatagagct agatgtattt ggtgcattgt ggatcatttt
    12661 tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg atggagagca tggtgcctcg
    12721 tgcacctttt ccatctaagt aaacacttgg gtatttcatc gttgtttttg cacctaggtt
    12781 tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt gctcgtttgg ttactaagtt
    12841 gtacacattg tctgaccagt tttggatcgt tgtgtaacgg caatacgcat cttttcttgt
    12901 aaagatttct acgatggcgg catgcaggct gttacttgaa taagtaggtg ccgtacaacc
    12961 ttcgacatag tggatgcttg ctccttcgtc aacaatgatc agcgtacgtt cgaattgccc
    13021 cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga acatcgactt tgacgccttt
    13081 tggcacgtaa ataaaggtcc cacctgacca tacggctgaa ttcagcgctg ctaatttgtt
    13141 gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat aattcagggt attctttcaa
    13201 agcagagtca gtgtcagtaa agacgattcc taacttttcg aattcatctt tcatattatg
    13261 gtacacgact tctgattcat attgtgcgga agcaccggct agataagctc gttcagcttc
    13321 tggtatacca atcttttcaa atgtatcttt gattttttct ggtacgtctt cccagtcacg
    13381 agcgggtttg tcacttggtt tttggtaata tttgatggca tcgaaatcga tgtctgaaag
    13441 gtcggggccc catgtttgca ttggcatttt gttaaatgtt tctagagatt tcagacggaa
    13501 ttcaagcatc cattctggtt cgcctttgat tcttgacatc tcacggatca cttcttctgt
    13561 caagccttct cctgtactga aaaccggttc gacatcatcg tgaaacccga atttatattc
    13621 ttctaattct ggtacgccca tgttctcact cctcttcctt gtggtaatga tggaaagctg
    13681 cggtaccatc gttttccatt gcttttccta gtgctttcca ggcaagtgtc gcacatttga
    13741 tccgagcggg aaatttagct acaccaccaa gcatagcagc atcgcctaat tggtcgggat
    13801 ctttgacttc ttttccttgg acaagctgta gaaagtctac agttaaatgt tctgcttctt
    13861 ctattgattt tccgatgacg gcgtctgtca tcatcgaggc acttgccgta ctgatggagc
    13921 agccactgcc gtcaaaggcg atatttttga tgattccgtc ttttacatcg acttgcaatt
    13981 cgatcacatc tccacatgtg gggttgttca attcgatctt gcttgtcgag tcagtcaagc
    14041 ttccacgatg atgcggatgg gaagaatgat ccaagatcac ttgacggtat aaattgtcta
    14101 atttagatag tcccatgttg gaaaaactcc tttgttgcca tgattgcttc aactaaacgg
    14161 tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc gagcagtcgc tgatacttca
    14221 aggtacttca atagcggttg cgcacaatga tgacctgctc tgacggctac cccttccatg
    14281 tctaaagctg tcgcgacatc atggggatgt aacccatcca aattgaatgc aacgactcct
    14341 gtccgaaaat tcgggtcttg tggaccataa acagtcaatc catcaattgc ttgtaatttt
    14401 ggcaacacat aagcggctag ttcttcttca tatcgatgga tattgtccat accgagtttt
    14461 tgcaaataat cgattgcatg gcctaatgcg atagcgccgg cgatatttgg tgtccctgct
    14521 tcgaatttcc aaggtagttc tttccatgta ctgtcgtata attcgacaaa atcgatcatt
    14581 tcgccaccaa attcaacagg ttccatttct tctaaccatt ttcgtttacc atataaaaca
    14641 ccaatacctg ttggtccgca cattttgtgt ccgctgaaag caaagaaatc acagtctagt
    14701 gcttggacat caatggccat gtgcggggca gattgagcac catctactac gatgaccccg
    14761 ccgttttcat ggacaagttc agctaattct tgaacaggat tgaagacacc taagacattg
    14821 gatacatgag caatggagac gattttggtt ttgtccgtaa tctttttcgc tgcatcctcc
    14881 atatccagga agccttcttc tgttagttcg atatatttca agacagcacc tgttcgtttt
    14941 gccaactgct gccacgggat aacattagaa tgatgttcca tgtaagagag cactatctcg
    15001 tctcctgctt tgatatagcg gtcgccgaag gttctggcta cccaattcaa gcttgtcgtc
    15061 gtaccacgag taaacagcac ttctgctgtt tcttttgcat ggatgaacgc ccgtactttt
    15121 tctcttgctt gttcataatc atgggtcgcg cgttctgcta acgtatggac accgcgatga
    15181 acattggcgt tatcgtgttc gtaatagtac gctaatgcct ggagtacttg ttttggcttt
    15241 tgggtcgtcg ctgcattatc cagataaact aaaggttcat cattgacgtc ttgaaataag
    15301 ataggaaaat cattgcgtag ctttttagca tcgatcatcc attcaacttc ccttcgatta
    15361 cttcgactaa ttctttccga acttgttcta cagggattgc tgttaagaca gaaccgagga
    15421 atcctcgaat cactaagcgc tctgcttctt ctttatgcaa tcctctactc atcagatagt
    15481 acatttcttc tggatcaact cgtcctacac tggcagcatg tccagcagtt acttcgtttt
    15541 catcgatcaa aagaatagga ttcgcatcac cacgtgcttt atcagaaagc atcaagacac
    15601 gactttcttg ttgggcatct gcaccttttg cccctttcaa gatgtggccg ataccattaa
    15661 aggtcaatgt tcctttttct cggatcactc cgtgttgcaa aatatgcccg atcgtatgag
    15721 gggctttatt ggttactcgt gtatcgattc cttggatttg tctgcccgag ctgattgcca
    15781 cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc tgagtcaaag tcagcgatca
    15841 catgaccgtc attcatgaca ccaattgccc agtcgacaga agcatctcgt aaaatgtgcc
    15901 cacggcgatt catataagaa gtaatatttt ctcctaattg atctacggct gaatatttga
    15961 tttttgaacc agctttagcg atgacttcca cgatgatatt acctgatgat tttgctactt
    16021 gttctttggt tgtctggaaa cgttccagat aagaaaattc actatgttca tctgccacga
    16081 tcaacacatg cttgaagaag tgctcatcgg atgcgccatc ttgaatgaat aaggactcga
    16141 gtggttcatc gatcacgaca tttttaggca catacaggaa aataccgctg ttcataaaag
    16201 ctacatggag tgcagtcaat tggtcttcgt tcatgtttac tgcttttttc atataatatt
    16261 cttggactag ttctggatat tcgatcattg cagtgaacaa gtcggtaaaa atgactcctt
    16321 tttctgctaa ttccacaggc agttgttcaa aaatcgtcca tgaaccttgt tggacaagca
    16381 caggattgtc tttcattgca tcaaatgccg gaatcgtgcc tgtttcactg atggtttgtg
    16441 tttctttcac atcaaataac gaccagcgat ggaatttcac ccgatcgata tgcggcaatg
    16501 gcagttcatc tgctttcgct agtgcgtctt gacggaaggt tctcatccaa tcaggttcgc
    16561 ctttacttaa agaaaaatca tttaccgcgt caagataatc gagccagttt ttttctttca
    16621 ttcttgctcc cccttacgct tcttcttctt tatagtcgat tcctaattct ttactgatac
    16681 cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc attccctgtc atgactactc
    16741 ggccttccat catgatgtga acgacatcag gtgtgatata gttcaataat cgttgatagt
    16801 gggtgatgat caatgcaccg aaattgtctc cgcgcatttc atttactcct ttagcaacga
    16861 ctttcaaagc atcgatatcc agacctgaat cgatttcgtc taaaattgca aatgtaggtt
    16921 ctaacatcaa caattgcagg atctcattcc gtttcttttc tccgccagaa aaaccttcgt
    16981 tcagataacg ttcagccatt tcttctggca tatttagtaa agccattttt tcatctaact
    17041 ttttcaaaaa tgccataaca gaaattttgt cgttttcatc acgtttcgca ttgatcgctg
    17101 cacgcatgaa ttccgcattc gtgatacctg gtatctcact tggatattgc atagctaaaa
    17161 ataagccaag tcgcgcacgt tcatctactt ccaagtctaa aacatttttc ccgtcaaaca
    17221 aaatttcgcc ttctgttact tcatagtttg gattgcccat gatagcggca gataaggtag
    17281 attttcctgt gccgttaggt cccatgatgg catggatttc tccggttttc attgttaggt
    17341 ttacgccttt aaggatttct ttttcctcga tcgacacatg tagatttttt atttctaaga
    17401 cagacatgca ttataccccc aaattctctt tcgttctcac tcattttaga ctaattgaga
    17461 atgaagcgca atgaccttcc ttactaaaat caaatatctt ttatttatta ttaaacaatt
    17521 ttgatttcta aattagaata ggtataaaaa agatgagaaa taagccatta cagacttatt
    17581 tctcatcttt tttgttccat accttatttt gcatcagcct ttactggttt aacagagccg
    17641 ccccatttgt ctgtgatcca ttcttggact tctttgcggt gaagcacttc aatcagtttt
    17701 ttgattttgt cactgtcttc atcaccttta cgagtcgcaa taatattcac gtaaggagag
    17761 ttgtcctttt caagcaatac ggcatcttct aatggattca accctgcgcc gtaagcgaag
    17821 tttgcgttga ttgcaactag gtcaccttca tcattttggt acgtagaagc taaaagtgat
    17881 gggtcgatcg tatggttgaa cttcaagttc ttagggtttt cttcaatatc atcgaatgtt
    17941 gctgtttcca gatctactcc atctttgact ttgactaagc cagcatcttg tagaattgtg
    18001 atgatgcgtc cccagtctga ttcagaattt gaagtaatga ttgttgcacc atcttttaat
    18061 tcggaaacat ctttgacttt tttagagtac aagcccattg gttcgatgtg gacagcaccg
    18121 gcattgacaa agtcatatcc tttttctttg atttctttgt tcaaataagg aatatgctgg
    18181 aagtaattgg catcaatgtc gccttcttcc aaagattggt tcggtaatac atagtcatcg
    18241 aatttgacga tttcaagatc gattccttca tctttcaata atggtttcac atgttccaaa
    18301 atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg tcgtgtcgtc tgctttattt
    18361 gctgaatctt tgctgtcgct gttgttaccg catgctgcta atcctacagt cagcaaaagc
    18421 gctgctgcaa atccaaaaat tttctttttc atatcctaca tccccctata tcctattatc
    18481 ttttgtctgt tttcttagtt aagaaatcac cgattccttg aacgataaat acgattagta
    18541 aaataattaa agtcgcaacg aatgtcactg ttagattacc acgctggtag ccttcctgcc
    18601 aagcaagacc tccaagtcca cctgcgccga tcgcgccggc catcgcagta aatccgatca
    18661 tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg aatactttca ggtatcaaga
    18721 ccttccagat gatttcccag taacttgctc ccattgcatc tgctgcttcc agtacaccag
    18781 ggctgacttc acgaaaagca atctctacta gacgggcata aaatggtgct gcggataaaa
    18841 caagagctgg aatggctgct tgcgcaccta gcatagtgcc tacgattgct ttagtaaatg
    18901 gaatgatcaa aaccatcagg atcataaatg gcgttgaccg gaaaatatta ctgatgatcg
    18961 atacgattcc ataaatgatt cgagcatacg gtttgttttt gcgtccaata gagtaaagga
    19021 tcaatcccaa aactaatccg atgatcgtca ctaagaccat tgagattaat gtcatatata
    19081 gtgtatcaag gatagcctga ttcatcgttt ccgggttgat ttgtttaaaa tctagatatg
    19141 tttctgaaaa gcttttatcc acgatggatc acctcaattc ctactccttg ttctttgatg
    19201 aacacttctg cttgatctaa ttctttggtt tcaccagtca tcaagacagt caatgaacca
    19261 aaggaacctt ctttcacttg tttgatcttt ccatacacaa cgttgacatc gatgttatag
    19321 cgtcgaatcg cttgggaaat gactggctcg ttggcatttc tttcgctaaa ggtcaaagtt
    19381 gccaaacggc cgcttggatt ttctttgatg agttcagcta atagttctgt cgaatcagct
    19441 tctggttcga tgtcttgttg tacaaagcgt ttggttactt cttttttcgg ttgtctgaag
    19501 acagtcaata cgtctccttc ttctacgact ttccccaact ccatcacagc gactttgttg
    19561 cagattttgc ggatcacatt catttcatgt gtgatcaaga cgatggtcaa attcaatttc
    19621 ttattgatcg ctaacaataa gtctaatact tcttctgttg tttgcgggtc caatgcgcta
    19681 gtagcctcat cacaaagcag gatctcagga tcatttgcca atgctcgtgc gataccgacg
    19741 cgttgtttct gtccgccgga aagttgtgaa ggatatgctt gtccgcgtcc ttctaacccg
    19801 actaaacgca acagttcttc tgcccgttct ttacgtttgc tctttggtac gccagctaat
    19861 tccagcggca gctggatatt ttcaagtaca gttcttgacc ataacaggtt gaaatgctgg
    19921 aagatcatac cgattttctt tcggaatgta cgtaattctt tgttttttaa ttttgtaata
    19981 tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct caagtccatt caggagacgg
    20041 actaatgtac tttttcctgc gccagaatag ccgacgattc cataaatatc ccctttttca
    20101 acgtttagtg aaacgtcatt gacggcttgg atgtttccgt ttttcccatt gaatgtcttt
    20161 ttgacattcg ttagttggat taatgacatg ccattcacat ctttctctta taatttttca
    20221 aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac gaaagcttga acttccgtgt
    20281 taccaccttg atttaccgtt acttcgcaat aacagcctct tccagtactt ggacttagct
    20341 tctttccgta tactgtcgcg ctgtaacggg cactcccgta gtaagtttgc ttacgctcgc
    20401 ttactctgct ctaagaccat cttccatctc attttcgtta cccattttca gcaaacaggg
    20461 ctctcttcaa acgtccgtga catgtactct tcttttcaaa gctttctcgt ttcatatagg
    20521 attgcttatg attatagcaa ctggaagaat cagtgtcaac ttttttcaag tgattttctg
    20581 attttataat tcgtcaaatt cttttggatc aacatctttg aaacttaaga tccacgcatt
    20641 catttcatct gaatcattca agatattgat atcttcaccg attttatcat taacagaaga
    20701 aactgtaccc gttagcgggc tggcaaattc attgaccgct ttttccgctt caacttcgat
    20761 cagaggttca tcttttttta gcgattgtcc tgcttttggc agattggcaa acgtgatgtc
    20821 tccaagttct tcttgcgctt catttgtcaa accaacacag tattcttttc cattaaataa
    20881 gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc attttatatc agttctcctt
    20941 cataaatttt ttcattgaat ccaattgcaa caagctggtc ctctcgtaag atcagtggtc
    21001 gcttgatcag cataccatct gtcgataaga cttggctcgc ttttgaagcc gtaaactcgc
    21061 ctacttgatc tttcagtcca agttcccgat atttcatccc gcttgtgttg aacaggcgac
    21121 ggattggtac ttgactcgtt tctagccaag tctcaatcgt tgaggccttc ggtacttctt
    21181 tgatcatatc aacggtttga taactaacgc catgctggtc taaccacgct ttggcttttt
    21241 tgcatgttga acatttcggg taccagtaaa aggtgtacaa ttttcttacc tccttgaaga
    21301 tcttgcaggt gcttcatttt ggtatctcat cgataatatc aacccgatac cgatcatgtt
    21361 ccctaaaatc gaggatcctc cctgactaat gaaaggcaat gggatccctg ttaaaggaag
    21421 taatccgata tttgctccaa tattctcaaa gacatggaac agcagcatca tgatcaaacc
    21481 gcttgcgata taggcgtaaa attcattatt cgtatcgaaa cacacccgaa tcatacgata
    21541 gattaaaata aaatacaaga aaatgacgaa tgcaccaccg ataaagccga agttttcacc
    21601 gataacggta aaaatcatat cggactcacg aacaggcaca tagatattgc ttacgttgaa
    21661 tcctttacca aacaagccac cggtaccaat tgccaaaagt ccttgggccg gctgataact
    21721 cattcctgac gtatcatgga aagggtccag ccatgaatca atacgtgcaa actgataaga
    21781 tttgaatcca actttataaa gcaagtctct tccgccttca gtcgtaacca agaagatcgt
    21841 tcctgctcca acgactactg ccaagattac tacaggtgcg atgatgcgcc aagagattcc
    21901 agacatcagg aaaatcccgc caaagatcgc aagaaagaca agcatcgttc caaagtcatc
    21961 ttgggccatg atcaatgcta aaaccggtat tgtcacgatc aagagcttcc caatcagcca
    22021 aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta tgtttagtta cgatcagtgc
    22081 catcatcaag atgtacgcaa tcttcatcaa ctctgctggc tggaaagtaa acgcaccgaa
    22141 tcgaaaccag tttttagacc ctgttgaagc agctaaattc tgatcataga atttgagcaa
    22201 gagtcccatt accactaacc ccaaaccata aaggtaagga gtcagtttcc agatccattt
    22261 ggaattcaaa tgcatgatga caacgattgc tgccgctcca actaaatacc agacaacttg
    22321 catccctacg cctttcaaga ctgctggatt gttcggatca tggctaagtg ctacatataa
    22381 tgagagcaga ccaatcaaac ataacagaaa gactggtaaa atcacgccat aatcaatacg
    22441 attatcgttc gaagctttta attttttttc catgtttcat cccttttctt aaaaaccata
    22501 ctgctgatta ttatagcggt ccagttagat aatggaaagt ctttccatag acatttggga
    22561 aaaattaact ttccgtttgt gcagttgctt gagcaaatgc agtacaaaag cctactgctt
    22621 atctttagtg tatcatagaa ctttttttga tacatgcaat tatctaccag ctgagattct
    22681 cctcgctgtg agaagtttct gtgctttcat tgtatcgaag gatctcttcg gcaatctcta
    22741 gcagctgatc agaaaccaga ttggttcgtt ggatgaaata aaaatatcga tggaaattca
    22801 acggagtcat tggcacattt tctggaacag agcgcttaga caaaatcgag cgaccgattc
    22861 cttccttcag caacgcaaca attacatcgt tacttttcac gatcatcttc ttatcttgga
    22921 tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac accagagttt gcttctctta
    22981 caagccacaa tttgcttttt gggtccccag cgatgactag ctggtcttcc atcaaagagt
    23041 atctttgtac gccggaagtc tctaatggct tttcgataaa accaaaatcg gattcatgtt
    23101 tcgctacttg ttctaatgat tcatgagaat tcgtgatttc gatctcaaaa tgcactttag
    23161 gaaacctttt gatcagatgt ttcatcaact gaggcaaaag atagacagca aaggtgtggg
    23221 aagcaacgat ccgacatact tcttcttcat tttgttcatt gagcaatttt tgctgagttt
    23281 cttcccattc ttctaacaaa accagtgtcc gttcatataa taagtcggct tgcttagttg
    23341 gaagcatctc ttttcttccg ttacgttgaa aaagatcgat attcaattcc cgctccaact
    23401 gtttgatttg attcgagact gctggttgag ataaaaacag tacttctgct gctctggaaa
    23461 aatttcttgt ttcatatact gctcgaaaag tcgttaataa cttaaacatg gcaccacttc
    23521 cataactttt tatgataatt actataaaaa ctatttgatt taaatataca aaaaaccatc
    23581 tataatagaa atgtacaaca aaacagttgc tgatccaact agtaacttag atgaagacaa
    23641 tcagctgtct gcttgctcgt aatcatacat tcctattcat tttacaatgt attcgttcat
    23701 cttggtacta aaatctatta aataaaaagg agaatattat atatgaagaa ctttttgaaa
    23761 attattccga tcccgatctg cgggttgatc cttgggttag cttcccttgg aaatctgttt
    23821 aaagactatc accatgttgc tttaggcaat ctaactggcg gtatctctat gattcttatg
    23881 atattcattt ttgttaaatt gtttatctta ttcgaacaca cgaaacaaac gttgcatgat
    23941 ccaatcatcg cctccgtttc accaactttt acgatgtcac tcatggttat ttgtacttat
    24001 tttgtttcat atgaaagtat tgcaccaatt gtcaaattca tttggctgtg cgctgttatc
    24061 ttccaagtga tccttgtctt ttatttcaat tatcaccatg ttatcaaagc tgatctttca
    24121 attgaagcaa tttatccaag ctggtttatc atttacgttg gatttggggt catcactgta
    24181 actgccggga acttcttccc acttattgga aaaattttct tttggatttc attgatctgc
    24241 tatgcgttct tattgccgat catcatcaaa cgtgtctttt tcgtaagaaa tatggctcat
    24301 ccgacacgtc cgctgattac gatcgttgca gcacctggtt ctctttgcct aacaggttat
    24361 cttaaaaact ttgctgaacc taatttttat cttgtcgtag cattgttcgc tttatctcaa
    24421 gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt tcaagcttcg tttttatcca
    24481 agctatgcag cctttacatt cccgcttgtt atctctgcaa cagctatatt tactactgtt
    24541 cattacttca acagtttagg tatctatagt agcgtattgg atttcttaaa agttgcggaa
    24601 tgtctgtttg caagtgtagt tgtcttctac gttcttgctc attacgtacg tttcttaatt
    24661 aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa tcgcttaata atcgattcaa
    24721 taggttgtga aaaagctgtt ctgcatcaag taataagaac ggccaaacaa aaacagctgt
    24781 tcatgttttt gtactggttg ggcttattac cgcagatgca agcttttgaa caccgtttac
    24841 caagaggtcg taaaagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattgc
    24901 ttctcaaatt tttgtgaaat ttcggcttat ttccgtagaa gctacttctg gaacaccgtt
    24961 tattcgaaac aagaggtcgt acaagacttt tgtcacgacc tcttgttctt ttgtcaaaat
    25021 actggtttag tgctactatg ttcgagtatg cttttagtat ttattgacga aaggaaccga
    25081 tcaaatgaaa tcattgtcac atccaataaa atctgtaaag gattcagcgg tccgcgaaat
    25141 cttacccggt ttactgcttt cttttgccat cgctgccttc agcaggcttc ttgctattta
    25201 tctgccacag ttaggtggag cgactattgc tattttattg ggtattttat taggaaatac
    25261 tttttttaaa cagcctgttc tagcaatcgg cacaaaattt gctgaaagca aactattaga
    25321 attctctgtt tttttactag gtgcaactgt cacagctcaa acgattgctc aaattggtgt
    25381 aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact atcatcggtg cttacttgat
    25441 tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg atggctggag gaaacgctgt
    25501 ttgtggttct tctgctattg cttcaatcgc cccttccatc catgcgaaag aggaagaaaa
    25561 aggacagatc atcacactag tcaatctact gggtactgtg atgatgttga ctctcccttt
    25621 tcttggatta cagttgtttg gtggaaatct cattgaaaaa agtgcacttt tgggtggtac
    25681 actccaatct gttggtcaag tagtcgctgg ggcaagctta cttgacaatt ctgttgtcga
    25741 attcgccatg ctatttaaga ttctacgtat tatgatgctc gttgttgttg tctatctgtt
    25801 tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa acgccaactg atacgcagaa
    25861 aaaacgaaaa ttaccgcttc cttggtatgt attagggttt gtgatctttt gtttggtcaa
    25921 cagcttgttt tctttacctg tagttattga ccaatcggca cattttatca gcagctggtt
    25981 tgagatcact gctttagcgg caattggtct gcgtttagac tttgcaaaat tcatgaaaga
    26041 aggtacacgc ttcttatctt atggattagc tgtcgggctt ctccaaacag tgatggcttt
    26101 actatttatt tggctgatca aagtttagaa tgaacatgaa aaaacagttg tgattctcgc
    26161 atggaacaat gtccgaatca caactgtttt taattttgca gatatccttc taacaacaac
    26221 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
    26281 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
    26341 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
    26401 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
    26461 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
    26521 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
    26581 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
    26641 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
    26701 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
    26761 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
    26821 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
    26881 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
    26941 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
    27001 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
    27061 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
    27121 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
    27181 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
    27241 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
    27301 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
    27361 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
    27421 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
    27481 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
    27541 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
    27601 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
    27661 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
    27721 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
    27781 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
    27841 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
    27901 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
    27961 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
    28021 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
    28081 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
    28141 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
    28201 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
    28261 tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
    28321 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
    28381 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
    28441 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
    28501 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
    28561 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
    28621 gcagtccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
    28681 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
    28741 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
    28801 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
    28861 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
    28921 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
    28981 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
    29041 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
    29101 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
    29161 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
    29221 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
    29281 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
    29341 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
    29401 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
    29461 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
    29521 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
    29581 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
    29641 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
    29701 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
    29761 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
    29821 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
    29881 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
    29941 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
    30001 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
    30061 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
    30121 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
    30181 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
    30241 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
    30301 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
    30361 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
    30421 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
    30481 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
    30541 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
    30601 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
    30661 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
    30721 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
    30781 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
    30841 gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct tcctgaccac
    30901 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
    30961 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
    31021 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
    31081 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
    31141 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
    31201 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
    31261 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
    31321 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
    31381 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
    31441 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
    31501 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
    31561 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
    31621 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
    31681 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
    31741 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
    31801 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
    31861 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
    31921 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
    31981 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
    32041 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
    32101 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
    32161 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
    32221 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
    32281 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
    32341 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
    32401 tccagtgcat ttggttatcg cggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
    32461 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
    32521 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
    32581 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
    32641 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
    32701 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
    32761 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
    32821 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
    32881 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
    32941 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
    33001 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
    33061 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
    33121 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
    33181 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
    33241 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
    33301 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
    33361 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
    33421 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
    33481 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
    33541 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
    33601 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgtcaat
    33661 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
    33721 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
    33781 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
    33841 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
    33901 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
    33961 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
    34021 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
    34081 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
    34141 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
    34201 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
    34261 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
    34321 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
    34381 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
    34441 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
    34501 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
    34561 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
    34621 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
    34681 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
    34741 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
    34801 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
    34861 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
    34921 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
    34981 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
    35041 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
    35101 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
    35161 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
    35221 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
    35281 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
    35341 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
    35401 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
    35461 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
    35521 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
    35581 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
    35641 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
    35701 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
    35761 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
    35821 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
    35881 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
    35941 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
    36001 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
    36061 agctgttctt gtgataattg gctaaggaag taagtttgat ctttattatt gtctactcca
    36121 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
    36181 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    36241 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    36301 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    36361 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    36421 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    36481 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    36541 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    36601 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    36661 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    36721 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    36781 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    36841 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    36901 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    36961 tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
    37021 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    37081 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    37141 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    37201 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    37261 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    37321 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    37381 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    37441 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    37501 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    37561 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    37621 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    37681 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    37741 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    37801 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    37861 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
    37921 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    37981 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    38041 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    38101 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    38161 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    38221 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    38281 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    38341 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    38401 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    38461 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    38521 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    38581 ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat aaacaaagac
    38641 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    38701 gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc ccagcgatcc
    38761 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    38821 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    38881 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    38941 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    39001 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaat
//
LOCUS       DANKMN010000018        37392 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000018 DANKMN010000000
VERSION     DANKMN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..616
                     /locus_tag="PW224_000920"
     CDS             <1..616
                     /locus_tag="PW224_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2907627.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            613..1386
                     /locus_tag="PW224_000921"
     CDS             613..1386
                     /locus_tag="PW224_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2907628.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1383..1781
                     /locus_tag="PW224_000922"
     CDS             1383..1781
                     /locus_tag="PW224_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL2907629.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1966..2637
                     /locus_tag="PW224_000923"
     CDS             1966..2637
                     /locus_tag="PW224_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907630.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2696..3136
                     /locus_tag="PW224_000924"
     CDS             2696..3136
                     /locus_tag="PW224_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907631.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3148..3816
                     /locus_tag="PW224_000925"
     CDS             3148..3816
                     /locus_tag="PW224_000925"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2907632.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3880..4503
                     /locus_tag="PW224_000926"
     CDS             3880..4503
                     /locus_tag="PW224_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL2907633.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4559..4945
                     /locus_tag="PW224_000927"
     CDS             4559..4945
                     /locus_tag="PW224_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL2907634.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5158..6549
                     /locus_tag="PW224_000928"
     CDS             5158..6549
                     /locus_tag="PW224_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL2907635.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7160..7528
                     /locus_tag="PW224_000929"
     CDS             7160..7528
                     /locus_tag="PW224_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL2907636.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7708..8649
                     /locus_tag="PW224_000930"
     CDS             7708..8649
                     /locus_tag="PW224_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL2907637.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8718..9449
                     /locus_tag="PW224_000931"
     CDS             8718..9449
                     /locus_tag="PW224_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL2907638.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9593..11773
                     /locus_tag="PW224_000932"
     CDS             9593..11773
                     /locus_tag="PW224_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL2907639.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11793..12275
                     /locus_tag="PW224_000933"
     CDS             11793..12275
                     /locus_tag="PW224_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL2907640.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12291..13052
                     /locus_tag="PW224_000934"
     CDS             12291..13052
                     /locus_tag="PW224_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2907641.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13183..14517
                     /locus_tag="PW224_000935"
     CDS             13183..14517
                     /locus_tag="PW224_000935"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL2907642.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14824..15309
                     /locus_tag="PW224_000936"
     CDS             14824..15309
                     /locus_tag="PW224_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL2907643.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            15302..15985
                     /locus_tag="PW224_000937"
     CDS             15302..15985
                     /locus_tag="PW224_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL2907644.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            16111..18756
                     /gene="polA"
                     /locus_tag="PW224_000938"
     CDS             16111..18756
                     /gene="polA"
                     /locus_tag="PW224_000938"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL2907645.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18801..19637
                     /gene="mutM"
                     /locus_tag="PW224_000939"
     CDS             18801..19637
                     /gene="mutM"
                     /locus_tag="PW224_000939"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL2907646.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            19601..20230
                     /locus_tag="PW224_000940"
     CDS             19601..20230
                     /locus_tag="PW224_000940"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL2907647.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            20282..20614
                     /locus_tag="PW224_000941"
     CDS             20282..20614
                     /locus_tag="PW224_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2907648.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20926..21417
                     /gene="nrdR"
                     /locus_tag="PW224_000942"
     CDS             20926..21417
                     /gene="nrdR"
                     /locus_tag="PW224_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL2907649.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            21440..22813
                     /locus_tag="PW224_000943"
     CDS             21440..22813
                     /locus_tag="PW224_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2907650.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22813..23739
                     /gene="dnaI"
                     /locus_tag="PW224_000944"
     CDS             22813..23739
                     /gene="dnaI"
                     /locus_tag="PW224_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL2907651.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23868..24308
                     /locus_tag="PW224_000945"
     CDS             23868..24308
                     /locus_tag="PW224_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2907652.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            24452..25153
                     /locus_tag="PW224_000946"
     CDS             24452..25153
                     /locus_tag="PW224_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL2907653.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            25150..26271
                     /locus_tag="PW224_000947"
     CDS             25150..26271
                     /locus_tag="PW224_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL2907654.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            26286..27518
                     /gene="pepT"
                     /locus_tag="PW224_000948"
     CDS             26286..27518
                     /gene="pepT"
                     /locus_tag="PW224_000948"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL2907655.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            27650..28558
                     /locus_tag="PW224_000949"
     CDS             27650..28558
                     /locus_tag="PW224_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL2907656.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            28593..28865
                     /locus_tag="PW224_000950"
     CDS             28593..28865
                     /locus_tag="PW224_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL2907657.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            28876..29922
                     /locus_tag="PW224_000951"
     CDS             28876..29922
                     /locus_tag="PW224_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL2907658.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            30173..32782
                     /gene="clpB"
                     /locus_tag="PW224_000952"
     CDS             30173..32782
                     /gene="clpB"
                     /locus_tag="PW224_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL2907659.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(32933..33619)
                     /locus_tag="PW224_000953"
     CDS             complement(32933..33619)
                     /locus_tag="PW224_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907660.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(33597..34091)
                     /locus_tag="PW224_000954"
     CDS             complement(33597..34091)
                     /locus_tag="PW224_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2907661.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(34111..35331)
                     /locus_tag="PW224_000955"
     CDS             complement(34111..35331)
                     /locus_tag="PW224_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2907662.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            35478..37313
                     /gene="lepA"
                     /locus_tag="PW224_000956"
     CDS             35478..37313
                     /gene="lepA"
                     /locus_tag="PW224_000956"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL2907663.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        12417 a         6550 c         8102 g        10323 t
ORIGIN      
        1 gtttgttttt gcaccgttag aaggagaaga cctcgcttca tttctggttg gtgcggcaat
       61 catgatgatc ggaatgacgc tctttttgtt tggagcagat tattctatga tggaagtcgg
      121 agatctagta gggaaatata tgatcaaaaa gaaaagtttg accgttttgg tcagtttggg
      181 atttgctatc gggatcgtga tcacgattgc tgaaccttcc gtgcaagttt tgggacaaca
      241 agtatatgat atatctgacg gagagattgg cagagtgttg ctgattggga tcgtcagcgt
      301 cggcactgga gtatttctag cctttgcgct gcttcgggtc gtattcaagc tatcctatta
      361 tcaattgatg gctataggct acataggcgt tttgatcgcc tcatttttca ccagttccga
      421 atttatgccg attgcttttg attcgggggg agtgactact ggtccagtaa ctgttccatt
      481 tatcttggcc cttgccggag ggatgactag tatgatcaaa caaaagaaaa atgaaaatga
      541 cagttttgga atggtcggta ttgcttcgtt agggccgatt cttgccgtga tgattttggg
      601 ggtgatcttc caatgagttt ctggcagcaa gtgatagatg gaatcgaaat cgttaccatg
      661 gaagtcattt tggcagtggc acctatcgta atcctatttg ttttaatgaa tatcattagt
      721 tttcgtatgc gcaaaaagcg tttctttgac attatgaaag gtttcttgat aacgactatt
      781 gggttgatcc ttttcctgca cggagtcaat atggcttatg taccggtagg acagcattta
      841 ggcagtgcta ttgcgggatt gccttacaat tatatactgg ttccccttgg ctttttaatg
      901 gggttcttag tagggtttgc tgagccggcg attcatgtaa tgatcaaaca ggttgaagaa
      961 ttgagcgagg ggagaatccg atcgaaaatc atgctgatgg taatttcaat tgggatcggc
     1021 gcagcagtag gattgtctat gtggcgtttg ctcgtcggat tctctcttta ttatttcttg
     1081 atcccaggat acgggctagc atttatttta ggacgaaaag tggacaagat ctttttagca
     1141 atggcttttg ataacggtgg agtagcaacg ggcccgatgt gttcgacatt tattttatca
     1201 atgagcgtgg caatcgctgc acaaattgaa ggaagagatc cgatcattga cgggtttggt
     1261 gtggttgcaa tgattgcatt gacaccaata ttatccactt tagtactagg atatatttat
     1321 aaacgaaaag atgaaaaaga cagaatcgaa aaagaaagat tgcaaaaatt gggggaaagc
     1381 aaatgaaaga tcttgtagca ttgaatcttc acatgattgt gacgatcgtt gacggaggaa
     1441 tgggcgaaga tgttatcgac ttttcaaaag aagcaggtgc agcaggcgga acgatcttac
     1501 atggaagagg ttctggtgta catgattcag ggaaattttt aggtttggac atttcgccag
     1561 aaaaagatat tgtgttgaca ttagtacccg atagtttgac gaataaagta atggatgcga
     1621 ttggtcaagg catcaacatc gatattccag gtaatggtat ttgttttagt attgatgtag
     1681 acaaagtcat tggtatcact aagattcatc aatatcgaga aattatcgga atgcaagaac
     1741 ttttagatga aaataacgat agagaaaagt ctgaatcata gacaagcgtt tgaagaggaa
     1801 ataaacacaa acaagaaggc ggatgagcgt tatttccgct tttttgtttg tatttttctc
     1861 gtgaatactt atgaaatagt aaatttttat gaaattctac tctaagcatc agactttctt
     1921 ttcaaaatat gctattttaa aaatgatgaa aaaaggggga gaaaaatgaa ttttttagtt
     1981 gttttaaaag atactctgat tgagcgattt acatttctat atgttcaatt gcaggaaaat
     2041 aaaacattgc tgcatttttc accagagttc catctagagg ggctaacatt aggagaaatc
     2101 tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt ggaattacca
     2161 gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt tgaactgttt
     2221 ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc agcagaactg
     2281 caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt tagacgtcag
     2341 caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg
     2401 acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg
     2461 attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt
     2521 ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt
     2581 gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc
     2641 tttttctttt tggatttttt tgtcataata aatattagat gatggaggag ataatatgat
     2701 gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg
     2761 aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag
     2821 tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg
     2881 atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg
     2941 ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca
     3001 agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca
     3061 gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt
     3121 cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc
     3181 gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga
     3241 aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc
     3301 gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg
     3361 ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat
     3421 cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca
     3481 tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga
     3541 tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat
     3601 cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta
     3661 ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct
     3721 gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta tacattagca
     3781 gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag
     3841 agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac
     3901 tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta
     3961 aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct
     4021 actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac
     4081 cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg
     4141 gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca
     4201 acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc
     4261 gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt
     4321 catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg
     4381 aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg
     4441 gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag
     4501 tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat
     4561 ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa
     4621 ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa
     4681 ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac
     4741 atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg
     4801 tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt
     4861 acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa
     4921 ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat
     4981 cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc
     5041 gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc
     5101 tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg
     5161 acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat
     5221 cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt
     5281 atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa
     5341 tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt
     5401 atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag
     5461 ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca
     5521 ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga
     5581 gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta
     5641 tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt
     5701 agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat
     5761 ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt
     5821 ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta
     5881 tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt
     5941 aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt
     6001 ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc
     6061 tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca
     6121 aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg tttgtctatt
     6181 ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt cgcaccagca
     6241 gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat tgtaacaatc
     6301 agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact gatcgtttta
     6361 ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga accattaatc
     6421 gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt
     6481 gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg
     6541 aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat aagaagaagt
     6601 ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga gaagctactt
     6661 ctgaaacacc gtgaataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     6721 ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag
     6781 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tttcactctg agtcacaaag
     6841 agcaatttca aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg accgaggagt
     6901 tgtcaggaga acaccgttta ccagaggttg tgacttgttt ttagtcacga cctctttttt
     6961 tatttattcc atataaatac acattattta ttccgaaaag tgaaagcgca aataacaaaa
     7021 acaaccaact tatttattat taaagaaaag cgtatttaag taggacgatt ctgttgagag
     7081 ctttcgtaga ttttcccatt ttcctatggt aaactgacaa agttgagaat gttagaacag
     7141 atcgtttagg aggaaacaga tgtttacgaa tgaaataaaa atcatgcttt atgtggacga
     7201 tgtagaaaaa aatgcaacgt tttggagagc agttggcttt gcggaaaaag atcgtcaaga
     7261 aatggatggg acgcttataa ttgaagtagg agttgaggac agtcaaacct catttctgtt
     7321 atatgatcga gcatttgttg agtcccattc acctggcaca gctactagtg ccccatcttt
     7381 gatgttttac agtaaagata tctttggact atataaaaaa atgcagacgc aatcagtcac
     7441 attaggtgat atggtacagc ttggagaaga atatgtcttt aactttgctg atcccgatgg
     7501 caactacttt gcggttacag gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa
     7561 caggaaagcg aagtcttttt aattcaaata ataatattat gtaaacaaaa aactttccat
     7621 gcgtaattat acacaagcaa ataaatcgtc aaatgcgaac gccttcttta tactgtttag
     7681 gaacgaaaag aaggaaggaa gaaatacatg gattatcaag tactgcttta ttacaattat
     7741 acaaaaatcg aagaaccaga ggaatttgcc aaaaatcatt tagccttttg caaatctttg
     7801 gatcttaaag gtcgtattct agtagctgaa gaaggaataa atggtactgt gtctggaacc
     7861 gttgaaaaca cgactgccta tatgaaagca atgaaagctg atcctcgatt tgctgatacg
     7921 atgttcaaga tcgatccaag cgaaggacat gcctttaaga agatgtttgt aagataccgt
     7981 ccagagcttg taagtttaaa gctagaagag gacattgatc caaaccaagt gacaggtgcc
     8041 tatctttcac cgcaagaatt taaagaagct attttagatg aacagacagt ggtgattgat
     8101 gcacggaatg attatgaata tgacttggga cattttcgag gggctgtacg cccagatata
     8161 cgcagttttc gagagcttcc agactggatc cgcgagcaca aagaagaatt tatggaaaaa
     8221 cgggtagtga cctactgtac tggtggcatt cgttgcgaga agttttctgg ttggcttgtc
     8281 cgtgaaggat tcaaagatgt cggacagctg catggcggta ttgctactta tggtaaggat
     8341 cctgaagtcc aaggagaatt atgggacggc aagatgtatg tgtttgatga acggatcgct
     8401 gtcgatatca accaagtaga taaacaaatc gtcggtcgcg actggtttga cggaacacca
     8461 tgtgaacgat atatcaattg tagcaatcct gagtgcaatc gtcagattct tgcttccatt
     8521 gaaaatgaag aaaaacattt aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat
     8581 cgctatgttc aagaccatgg aatctctcca gaagaagcag aactgcggtt tacagcttta
     8641 attaaataga aaacaatagt tttgattgtc gaccatttcc aatatgcgct atactagcgt
     8701 tgaggatggt gatccacatg aaatcattga tttatgctat tggggatgtc catggcaaac
     8761 atgagctttt tgaaaaactt attcactatt atgatccaaa gatccatcag cttgttttat
     8821 taggggatct caatgatcga ggtcctcgta caaaagaatg ttttttatta ggaaagcaat
     8881 tagtcgaaga aacaggagcc atttacctcc ggggaaacca tgaagaatat tttttgcaat
     8941 ttctgcaatc accggaggac tggtatgcgc cttatatgag aaatggtgga aaagaaacgt
     9001 ttgaaagcct gattcaccca ggggcatcgg aggaatactc cccaactgaa atctcgatga
     9061 tgatccaatc gagatattca gaactgatta aatttttagt ccagcgcccc ttgtattatg
     9121 aatgggaaaa ttatcttttt gtccatgccg gtgtggattt gacaaagaaa gactggcgta
     9181 agacaagtct aagagacttt atttggataa gggaaccatt tcatgaaggg aaaaacaata
     9241 caggtaagac aattgtattt ggacatacga ttacaccaat gctccatgga gatatgcaga
     9301 caacagacct ctggattcag gatcataaaa tcggaatgga cggtggggct gtattcggag
     9361 ggtcattgca tggggttgtt tttgacaagg aaggtattct tcaagatatt gaatatcaga
     9421 acaacgaagg accttggcat ggaatctgac taatggaaag aatgaaggag ctgtgacttt
     9481 gttgcggctc tttttatttt tatagagaat attgtttctt tcagtagttt aacccaggat
     9541 aaaaagtatg atagaataaa gaagcaattt ttagcagaaa agaggcattt taatgacgga
     9601 aaatttgaac cagacgatca ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa
     9661 gcagttgact actgtactta atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg
     9721 ttaccgaaaa gaaatgactg gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg
     9781 gtatgcctat cttgaaaact tagaaaaacg taaaactgaa gtgattcggc taatcgatga
     9841 acaagggaaa ctgactcccg agttagaagc agaaatcacc caagccgtga aaatgcaaca
     9901 agtggaagat ttgtatcgtc catacaaaca aaaacgacgg acaaaagcaa cgattgctaa
     9961 agaaaaagga cttgaaccac tcgcgaagtg gctgatgcag cttacagatg gagaggtgca
    10021 atctgaagca gaaaaatata ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt
    10081 gcacggtgct catgagatta ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg
    10141 gattcggtct tatacgtata acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc
    10201 agatgaaaaa ggcgtatatg aaatgtatta tgactttgca gaacccgtcc ataaaatggt
    10261 ttctcatcga atattagcta ctaatcgtgg agaaaaagaa gatattctga aagtattctt
    10321 tcaagtagat gaagcagcaa ttttagctta cttggatcgc caaatagtca aaaatcctgc
    10381 ctctccttct tcttcattcg tcagagaagc ctatcaggat agttataaac gctttattca
    10441 gccagcaatc gagcgagaac tgcgcaatga attgactgaa aaggcagatg agcaagcaat
    10501 cgctattttc ggcgaaaacc tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt
    10561 tgtcctggga tttgatccgg cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac
    10621 agggaaagtt ttagcaatcg aagttattta tcctcataaa cctgcagcgc aagcaaaaag
    10681 agaagctgct ggaccagctt ttatccaatt aattaataaa tatcaagtag atatggtagc
    10741 tatcggaaat ggtacagcca gtcgggaatc tgaacttttt gtggctgagc agttgaaatc
    10801 cgccgaccat aaaacctatt acgctatcgt caacgaagca ggcgcttctg tttactccgc
    10861 tagcgaaatt gcacgaaaag aatttccgca tctacaagtt gaagaaagaa gtgctgtttc
    10921 tattgcccgt cgcctgcaag atcctttagc tgaattagtc aaaatcgatc ctaaagctgt
    10981 cggagtaggc cagtaccaac atgacgtttc tcaaaaacgt ttggctgaac aattggattt
    11041 tgttgtggaa acagcggtta accaagtagg tgtggatgtg aatacagcaa gcccgcagct
    11101 cttgcagcat atatcaggat tgaataaaac aactgcccaa aacattgtca tttatcgtga
    11161 agaaaacgga gaattcaccg cacgaactca attgaaaaaa gtgccacgct taggaccaaa
    11221 agcatatgaa caagcaatcg gattcttgcg agtccctggt gggaaaaaca ttttggacaa
    11281 cacaggtatc catcccgaaa gttattctat tgcaaaagaa atattaactt cagtccaact
    11341 ttcagaaaaa gagcttggta cagaagaagc agtagaaaaa ttgacacgat tatccgtaga
    11401 gaaactggct gaaagcctat cagtcggaga agaaacgtta gccgatattt tagctgggct
    11461 gacacaacct ggacgagata tgcgtgatga gatgccagcg cccttattac gtacagatgt
    11521 attgagtatg gaagacttga aaccagggat ggaattgact ggaacggtcc gtaatgtcat
    11581 tgacttcggc gcatttgttg atatcggtgt gaagcaagac ggacttgtcc atatatcgaa
    11641 actcagcaaa aaattcgtca agcatcctac agatgtcgtg tcagtaggag atatcgtaac
    11701 agtttggata gaacaagtcg atacgaaaaa aggcagaatc agtttgacga tgctttcccc
    11761 ttatgaagag taacaatgag gagtaacaaa aattgacaca agaagaactg cagcagttag
    11821 tagaagagat atcattaaaa agcttccagc ttcctttcaa gcaccaagca atgttcaatc
    11881 cacgcttacg tacaactggc gggcgctatc atttaaacga tcatcattta gattttaatg
    11941 cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt
    12001 gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt
    12061 atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa
    12121 agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg
    12181 atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa
    12241 gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa
    12301 gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag
    12361 agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta
    12421 tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg
    12481 tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc
    12541 attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg
    12601 atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca
    12661 ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga
    12721 atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac
    12781 aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga
    12841 tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag
    12901 tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag
    12961 attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag
    13021 agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc
    13081 aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta
    13141 atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa
    13201 ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat
    13261 gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat
    13321 ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg
    13381 acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa
    13441 ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg
    13501 agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta
    13561 ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg
    13621 caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca
    13681 ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc
    13741 gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg
    13801 tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt
    13861 gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct
    13921 tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa
    13981 cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg
    14041 aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag
    14101 atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca
    14161 acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc
    14221 catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct
    14281 gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag
    14341 attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta
    14401 tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa
    14461 aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac
    14521 aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat cttgccaagc
    14581 cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    14641 caatcaaatc aaaaacatga gaagctgttt tttcttggct gttcttatta tttgatgcag
    14701 gacagctttt tcacgacctc tgtaaactat aggaatctgt taaatcaagg gagcagaact
    14761 tgccttttac gctacttttg gtacaataat gtatgattaa aaataaactg gagggtttct
    14821 atcttgaaca taggtaagcc gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc
    14881 gactcgttat ccttaacaga atctattgaa gataaccagc ttttgctgta tttgggattg
    14941 acgaatgatg caaatccttt gtatatccaa catgactttg cacaaaaaac agaatatcaa
    15001 cagccaatcg ttccatcagt gatgctgatg gggatcatca caagtgcaat ttcaaagcat
    15061 cttcctggac caggatcaca tgttgtcaat ttttctgtca attttattga accggttttc
    15121 cattatgaaa cactgacttt tcagtttgag gtcataaaag tagataagat gaaggatgtg
    15181 gtgacgattt ctgtagaagc ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt
    15241 atggtccaac cgccacaagt tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg
    15301 aatgaataat ccaagtacat caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg
    15361 atttttaggt atgggacttg cccttagtgc agtcacttct tacttggctc tttgggtatt
    15421 tcctttgcaa ttagcaactt ttatcaataa ttttccacta ggctttgtgg gtctgtggat
    15481 cgttgaactg attttagtaa tggttctagg cgtaaaagca caaaagaatc cgagcttagc
    15541 aatcggtgga tttattgcct actctctttt gaatggcata acattagcgg ttactcttgc
    15601 atactacgat attggaacag taacgcaagc tttcttgaca gctactttga tgttcatggg
    15661 gatgtctgtt gtcggagtaa caacaaaaag agatcttagc ggggttggaa gagcaggaca
    15721 ggcagcttta atcggagtga tcattgcact agtcttgaac atcttcattt tgagaagtag
    15781 cgtagttgag ctgttcatct ccctactgtt agtcgtcatc tttgcaggga ttacagctta
    15841 tgataaccaa agcatccgta atatctatgt ccagacaaac ggaacagcac atagtggaat
    15901 tgctgtattc atggcattga agctgtattt ggactttatc aacttgttct tggcgtttct
    15961 gagaatcttt ggacggaaca actaaaaaag aaaaaaattc atcagttggg gctataccga
    16021 attatttggt atggtcccgc tttttctttt cgcattccgc aggattcttt gttagaatga
    16081 ataagaactt tacagtaagg agttaagaag atgacaaaaa ataaattatt actcgttgat
    16141 gggaacagtg tggcttttcg cgcattcttt gcgcttcaca attctcttga acgtttcaaa
    16201 aatagaaacg gtcttcatac aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt
    16261 atgcaaaaag agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa aacgactttt
    16321 cggacagaaa tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa
    16381 gaacaaatgc catatatacg tgatttgttg acaggactag gggtacagta ttacgaattg
    16441 cccaattatg aagcagatga tattatcggt actttggcag aaaaagtaga caaagatcaa
    16501 tttgatgtcg tcgtgctttc aggtgatcgg gatttgactc aattagctag taaagaagta
    16561 aaagtggata tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc agaacatgta
    16621 gcggaaaaat atgatgggtt gacgccaaaa cagattattg acatgaaggg actagccgga
    16681 gatgcctctg acaatatccc cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta
    16741 ttgaaacaat atggctcagt cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc
    16801 aaaatgaaag aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg
    16861 accatcaata cttcagcgcc tgtggatgtc tctatcgact cattgaagta tgaagggaaa
    16921 aatctcgata aactcgttcc gttttataaa gaaatggatt tcaatcagtt cctttctaaa
    16981 ttgaatatcg tagaagaaga agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa
    17041 ttcaaagagg aaatgtttac tacggatatg gcgttgtacg tggaaatgat gggcgacaat
    17101 tatcatacag aggaaatcgt cggagtagcg tgggggacag aaaagaaaat ttatgtcacg
    17161 aacgatctat ctgtattcga aaaccgagcg ttccattctt ggatcactga tccgactcgg
    17221 ctgaaaaaag tatatgatgc gaaacgaact tatgttgctt taaaccgcta tactggaaaa
    17281 acgaaaggaa tcgattttga tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa
    17341 agcacagata tcgaaggaat agctgctcat tacggataca acgatatcca atcagatgaa
    17401 gctgtatacg gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact cttttttgct
    17461 catttagcgc gaaaagtagc tgcaatcaat gcgttgacac caaaattgag ccaagaactg
    17521 gcagaaaaaa accaagaaga tcttttccgg aaaatggaac tgccattgtc tatcattttg
    17581 gcagagatgg aaataagcgg gatcaaagta gatgcgactc gcttacaaga aatgaagggc
    17641 gagttctctg ctcgcttaag agagatcgaa caaaaaatct acgaagaagc cggcgaagaa
    17701 ttcaatttga attcaccaaa gcaattagga gtgatcttat ttgaaaaaat gggattaccc
    17761 gttatcaaaa aaacaaaaac tggatattcc acagccgtag atgtactgga acagcttcgt
    17821 gaacaagcac caatcgtaga ggatatcctc acctatcgtc aaattgcaaa aattcaatct
    17881 acctatgtcg agggactgct gaaagtgatc caatcagatg gaaaagtgca tacccgttat
    17941 gtccagacat tgacccaaac aggacgatta agctctgttg atcctaattt gcagaacatc
    18001 ccgattcgat tagatgaagg gagaaagata cgtcaggcat ttgttcctag agaaaaagac
    18061 tggctgattt attcttctga ctattcacaa atcgagttac gcgtacttgc gcatatctca
    18121 aatgatgaac atttgaaagc tgccttccta gaaggtcaag atatccatgc cagcacagcg
    18181 atgcgtgtat ttggaatcga aaaggcagag gatgtcacac caaatatgcg taggcaggca
    18241 aaagcagtga attttggtat cgtgtatggc atctctgatt acggactttc acaaaatcta
    18301 ggaatcagcc gaaaagcagc acagcaatac atcgatacgt actttgaaaa atacccaggt
    18361 gtcaaagaat atatggaaag aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg
    18421 ctgtatcacc gccgtcgata tctgccagac atcaattcaa gaaattacaa tatccgttct
    18481 tttgcggaac gaacagctat caatacacca atccaaggaa gtgctgctga tattttgaaa
    18541 atcgcgatga tcgaattgga taaacgatta aaagaaacag gtcttcaagc tacgatgctg
    18601 ttacaagttc atgatgaact tgtcttcgag gtaccggaaa aagaacttga atcattagat
    18661 aaattggtga aggaagttat ggaacaagct gtttctcttc atgtaccact gatcactgac
    18721 agcagttggg gaaaaacgtg gtacgaagca aaataaacat tgtttaagac agagcaatat
    18781 aatgaaggtg aaaagaaaaa atgccagaat taccagaagt agaaaccgtt cgtaaaggat
    18841 tggaacggtt agtagtagga aaaacgattc aaaaagttca ggtcttgtgg cccaagatca
    18901 tcgagcaacc agagacaccc atctttgagg cttctcttgt tggcgaaacc atccaatcca
    18961 ttggacgtag agggaagttt ttaatttttc atctcgatca ttgtgaactg atctcacact
    19021 tgcgaatgga aggaaaatat caatttacga aagaaaatac accaatcgat aaacatacgc
    19081 atgtactttt cttttttgag gatggaagcc aacttcgcta caacgatgta cgaaaatttg
    19141 gtcgtatgac aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg
    19201 gacctgagcc tttgccagat agcttcttat tagcagattt cgcaaatgga ctgaaaaagt
    19261 cccacaaagc aatcaagcca ttgctgctag accaacgtct agtgactggt ctaggtaata
    19321 tttatgtaga tgaagcatta tgggaagcga agatccatcc agaacaaccg gcgaacacac
    19381 tgcgccccaa agaggtagaa caattacgtc tatcgatcat tgatgttttg gatcgtgcaa
    19441 tagaagccgg aggtaccacg attcgcagtt atttgaatgc attaggagaa tcaggcggat
    19501 ttcaagtagc actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc
    19561 cgatcgtgaa aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc
    19621 taaaaatcgc aaagtaggat ttgttttagg tttgacgggt ggaatagcta ccggtaaaag
    19681 tacggcagcc aaagtctttc agtctcatgg atttcctttg atcgatggcg atgtgattgc
    19741 cagagaaact gttgagcctg gaacgcttgc tctgaggaaa attgtatcag tgtttggcca
    19801 agaggtattg cagcctgatg ggcaactaga tcgagggaaa cttgggatga ttgtttttcc
    19861 atcaaaggaa aaacggcaaa aacttgatca gctattagat ccgtttatcc gaaaagcgat
    19921 aaaagatcaa atcgcctctc taagttccaa tcatccttta gtaattgtag atatcccatt
    19981 gctctacgaa ggtcattatg accactatat ggatgcagta gctgtcgttt atactacgcc
    20041 agaaactcag cttcagcgtt tgatgaaacg taatcaactg acgctagaac aagcgcagca
    20101 gagaattagt agtcaacttc caatcgaaga aaaaaagcag cgtgcggata ttttgttcga
    20161 caacaatgga acaaaagaaa aacttgtggg acaaatcgaa aattggcttt ttgctaatcg
    20221 ctttctttga gtatctgtgt caattttgct atcctgagta tatagaaagt gagggatagc
    20281 gatgggatca ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg
    20341 cgagatgctc aataaagaaa atctaatcga tgaaggtcgt acgcaatcag aaacagcaga
    20401 agaaaaagaa aagcagtacc aagcaagaat cgatgaaata gaagaccagc aggcagaaga
    20461 agaagcgaag cggcaagatt tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa
    20521 aacaccgctc ccagaaagtg accacattcc cgatgagcaa aaaatcgagg aacagcgaat
    20581 gaaacactat acaggaatcg aagagaagtt ataaaatagt aaaaaatcga gatgtgactt
    20641 gaagcagatg agaaggtgtt catctgcttc aagttttttg tgctagatga ctaaatcagc
    20701 ttttgtgtaa gttatttctc agtaatcaag gtgatcagta ttggaaaatt gaaatttagt
    20761 aaggacaggg gacacaatta cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt
    20821 ttgggtagga cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga
    20881 aagacacatc ctttatataa aatgcgatgc aagaggtgag acaaaatgag atgtcccaga
    20941 tgtcaacata ataattcacg agtaatcgac agccgtcaag cagatgatgg acgtgcgatc
    21001 cgtcgccgtc gcgaatgtga gaattgttcg ttccgattca ctacatttga acgaattgaa
    21061 gctgctccgt tattagtaat caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa
    21121 attttaagag gactcatccg ttcagcagaa aaaagacctg tagctatgga acaaatggaa
    21181 caaatcgtca accatgtcga aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg
    21241 aatctaattg gtgaatatgt gatggaagat ttagttgatt tagacgaaat cgcttatatt
    21301 cgttttgcaa gtgtttatcg ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa
    21361 gatatcgtgg ataaagcaaa agaaaataat aaagagaata ataaagaaaa caattaagaa
    21421 acaaaaagga ggcatttcct tggaaaatgc ttggaaagag cttcaaccaa aaaatattta
    21481 ccaaggaaga aaagcggctc cgctgagaga agacagcaag aacgccttgc tgtatcttta
    21541 tcagccacta atcggcggag aagccttgag tatctatttc accctgttat cagaaatctc
    21601 tcaagaatct ggcaaaggtc ctgaagggat gcatgcagat ctgcttagta gtctaggctg
    21661 cggtatcccg caattttatg atgcaagaaa aaagctagaa ggaatcggct tactggatgt
    21721 ttattttaaa gaagatagtg atctgggacc tcgttttatt tatgagctgt tagaaccact
    21781 cattcctaaa gcatttttca aagactctgt tctggctttt cttcttctgg aaaaagttgg
    21841 tgaacgacga ttccagaagc ttacccaatt ctttgaacct aaaaggcata cagcagaggg
    21901 atatcaaaaa atcacaaaaa aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc
    21961 tgctaatcaa gaaaacatcg aatcgctgaa taaatctttt gaagaatctg aatcagtagc
    22021 attacttgaa gcagattcga cattcgattg gcagtttttg aatgattggc tggcaaaaaa
    22081 acatattgca agtcttgatg atccaatcat caaacaagtc gacatgtatc atcagcttta
    22141 cggtttcgat gaattgacac ttggcgaact gatcagtcaa tcttatgact ttagtgaaca
    22201 aaaagtatct gaaaaagaac tgcaaaaagc cattttggca atgcagagac gtccggtgaa
    22261 gcaaacaaaa gaaacagttg cggtttctga aaacagtaat gaaccctcgt caaacgaaca
    22321 tttgacaaac gcagcgcttc agctgataaa agaagcacag agcattccac cgatgaaata
    22381 tctggaagca atcaagtcag aaaaaggcgg atatgtttct aaacaagaaa actggttact
    22441 gcaagatctt gtttctcaat caggtctttc cagtagcgtg atcaatgttt tgctgaatta
    22501 tgtactagtg atcaagaata atgctagctt aaatgccagt tatgtaaata cagtggcaaa
    22561 tgaatgggca caaaagaaaa tcgccacagc agaagaagcg atccagcaca ttcggcaaat
    22621 aagtaatcaa gcaaaacaat caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg
    22681 aaatgttcga cgggaaaagc ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat
    22741 cagcagcgag aaaaaagcag agattgaccg ccgttttaaa gaatacctgg caaaaaagga
    22801 gggtgatatc tagtggaaga cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat
    22861 tatcaagaaa agtatgctga aatgatcgct gaagtcttaa atgatgcgga tgttcaagct
    22921 tttctaagcg cacaccaaga agaattgaca caagcggata ttgaaaaaag ttacgctaaa
    22981 ttgtatgaat tcgtgcaaga aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct
    23041 cccggctatg agccgaagct gacgttgaat tttcattatg tggatgtcac ttatgtacca
    23101 acggaagcat tgttagcacg gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg
    23161 gatatgccaa aagatattca atcggcttct tttgaaaact ttgaacgtac agatggacga
    23221 ggatatgcga gcttagaagc cctagatttt gtagagaaat atactgctga tccaaaaggg
    23281 tatcataaag gactgtatct agtaggaagt tttggtattg gaaaaacata tttattagga
    23341 gcaacagcga gagaattagc gattgctgga ttcaccacga cgctagtcca ttttccgaca
    23401 tttgccgttg agatgaaaca ggcaatcgga aaagatcaag tggcagaaaa gttagatgca
    23461 gtgaaacgtt cacctatttt gatgatcgat gacattggtg cagatgcgat gagtagttgg
    23521 atacgagacg aagtattcgg tgttatcctt caatatcgga tgcaggaaca gttaccaacc
    23581 ttcttctcat cgaattttac aatggatgaa ttggaacagc atttgtccgt cactcaacgt
    23641 ggagatgaag agccgctaaa agcgaaacgg ataatggaac gcatacgtta tttgacaaaa
    23701 gaaatcgaaa tgactggtcg caaccgacgc aatggttgaa cagttgacaa gtccaactga
    23761 catttgacct agtagtaaaa gcaggtcagc gaatcattat atggacagaa atgaccggag
    23821 acaatcaccg caacaagtaa tgcagaaact taaacggagg attttttatg agggctattt
    23881 tttctagtat atctgtagtt ggtctatttt ttcttttagc aggctgcggt agcgcagata
    23941 gcaacacagg ttctgttgat tcgattgatg cgggtcaaac agtaaaaaca agcagtacga
    24001 aaacggagag acaaagcata tttgtcggaa cgattgaaag tatcactcaa gtagaagcag
    24061 aagaagaaac gcttcagttg aaactcgtcg atgtcatggc caaagaagat ccagcgaata
    24121 tgggaacgtc attcagcaat gacggagtga tcttaaatgc tactgctgaa aaaataagcg
    24181 tggacataaa tgaattgaaa atcggtgaca aagtccaatt tacattagtt gagatgccga
    24241 tcatgacgat gtctatccca ccgcaagttc ctggagattc gattattgat gtttcattga
    24301 acaaataacc aataacattt tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg
    24361 tatatttttt aaggagatat gggaattttt ttattcctag tctccttatt ttatttggaa
    24421 aatgctataa ttatctagaa ggagtgaagc tatgaaccat ttagatttat ctcagcgctt
    24481 agccgcagtg ggtgactttg tccctgaatc cgctcgttta gcagacattg gatcagatca
    24541 cgcttactta cccgttgcat tgatattaag aggaaaaatc gaatatgcag ttgctggtga
    24601 agtcgttcaa ggtccttatg aatcagcacg taagcaagtc aaaaaagatg gattagaaga
    24661 ccgtatcacc gtacgccttg caaatggtct agatgctatc cagccacaag atggtattac
    24721 agctgtcacg attgcgggta tgggaggttc actgatccga gatatcctag aagctggcaa
    24781 gttgaatcaa cgcttaagcg gacaagaacg tttgatcctt cagccaaaca tcggagagcg
    24841 tacattacga tcttggctgc aagaaaatca gtataaaatc caatcagaaa aaatacttga
    24901 agaaaataaa aaaatatatg aaatcattgt agccgaaaaa gaacaaaaac cagttactta
    24961 caatgaaatg gagctgatgt tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa
    25021 atcaaaatgg acaagagagc ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc
    25081 agctaatcaa gaggcacgtc aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt
    25141 tttaaagcca tgacaatcag cggacgcact ttcatccaaa agtttgaaag ctattgtcct
    25201 cagtggctag ctgaagaagg agacccagtc ggactgcata tcggaacatt ggataagcca
    25261 atccaacgag taatgatgac attagacgtt cgtccagaag tggtcgaaga agcaatcaag
    25321 aaaaaaatcg atttgctgat tgctaaacat cctccaatct ttcgtcctgt aaaacggctg
    25381 gttactgatc agccacaaga aaaaatgtat gcagatttgt tgaaacatga tattgccgtc
    25441 tatgcagcac acacgaacat ggatattatt tgggatggac tgaatgactg gttttgtgaa
    25501 cttctcggta ttgaagtgga gagttattta gtaaagactc atgagattca ttataaaaaa
    25561 ctggccgttt atgtacctgt ggatcatgca cagaaaatgc gtgaagtatt agctgcagct
    25621 ggtgctggta cacaaggtga ttataccggt acaagtttta cctcgattgg acacggtcga
    25681 tttactccgg aagctggtgc acaaccagcg attgggaaag tagggaagac agaacaagtt
    25741 caagaggcaa aagttgaggt tattttgccg gaaactatcg aaaaacaagt gattcaagcc
    25801 atgcggtcag ctcatcctta tgaggaacct gcttatgatc ttttcgctat agatgaacca
    25861 gtcgaaatgt ttgggttagg acgagtaggt gaattgccgc aagaaatttc tatagaggca
    25921 tttgtagaac aagtaaaaga agcgttccaa ctagatggac tgagaatagt ccagccaaaa
    25981 aatgcaaaat cctctgtgaa gcgcattgcg atttgcggtg gaagcggtga aaaattttat
    26041 ccgcaagcaa tcgcccaaag agcagatgtc tatatcacgg gagatatcta ttatcatacg
    26101 gctcatgata tgcaaagcgc tgggttgatt gcaattgatc ccggtcatta tattgaatcg
    26161 ttatgcaagc aacgatttat cgagaaattt gaatcttgga aacaagaaga aaattgggat
    26221 attaactttt tcgtttctga aacaaataca aacccatttc aatttaatta aaaggagaga
    26281 atactatgta cgaaaatctt ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct
    26341 ccgatgcaac aagcaaaacg actccatcga ctcagacaca agttgctttt gcgcaagaat
    26401 tgaaaaaaga attagaagag ttaggcttag ctgatgtttt ctataatgaa gataacggat
    26461 tcgttatggg aacattacca agcaatgttg atcatgaagt gcgttccatt ggatttattt
    26521 cccacatgga tacagcagat tttaatgcag taggagtcaa tccacaaatc attgaaaact
    26581 atgatggcga gtctacgatc gtactagatg cggaagaacg ctatacattg aatacaaaag
    26641 attttccaaa tttgaaaaat tatgaaggcc acacattgat cacaacagat ggcagtacat
    26701 tactgggagc agatgacaaa tcagggattg cggaaatcat gactgcaatg gagatcttga
    26761 tcaataatcc ttctatccct cacggtacca tcaaagtggc ttttggtcct gatgaagaaa
    26821 tcggtgtcgg agcggataaa ttcgatgttg agcaattcga tgtagatttt gcttatacag
    26881 tcgatggtgg tcctttaggt gagttgcagt atgaaacgtt tagtgctgct caagcaaata
    26941 ttacgatcca agggaaaaat gtacatccag ggacggcaaa agatacaatg atcaatgctc
    27001 tacagttagc aatcgatttt cataatcagc ttccagccga tgaagttcca gaaaaaacgg
    27061 aaggatacga aggtttcttc catttgatgg cattggatgg gaatcctgaa gaagcttcta
    27121 tgagttatat cattcgcgat catgatcgtg aaaaattcga agcacgcaaa gcacaaatca
    27181 cacatatcca agaagagttg aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt
    27241 atgatcaata ttacaacatg aaagaaatca ttgaaaaaga tatgagcatc gtggaattag
    27301 cgaaacaagc aatgatcgag cttggtatct ctccagtgat cgaaccagtt cgtggaggaa
    27361 cagatggttc aaaaatctct tacatgggta ttccgacgcc aaatctattt gccggaggag
    27421 aaaatatgca cggacgattc gaatttgttt ctttacaagt aatggaaaaa gcaacagacg
    27481 tgattgtaaa aattgcagaa ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg
    27541 acaaggcgtt cgtcccaatg atggaatgtc atctgtctaa ggctaaaggg caggacaatg
    27601 gaattggttg tcccgccctc ttttttgaag agtaaatagg aggtaaagaa tggaaaagct
    27661 gctagttttt tataacaaaa gttcagggaa agatgagggt gagaaattag cacgctggtt
    27721 tcaagattat gtaaaagaaa agcagccaga attatatgta gaactagctg aaactggccc
    27781 atcgattgat gatgcggaat tagtcaagaa agcagaagcc atagaagctg atactttagt
    27841 aattataggt ggagacggca cgattcatca tatcgtccaa gcgttccaaa aaacattatc
    27901 tcaataccat gtcggattac ttccaggtgg aactgtcaac aaccttgcac gtgttcttga
    27961 gattcccctc aaaaaagaag aagctgctga cgtgatcctg gaaggcaatg tacggaaaat
    28021 agactatggt caagtcaatg atgaagtcat tatctctaca ctgacaatcg gaatcttagc
    28081 agatacagca gtttggatca gtcaagaaga aaaacagcgt tacggctcgt ggatctttat
    28141 caagaagttt tttaagttat tgctgaagaa gaaaaaatat catttggatt tagaaacaga
    28201 cgaggagcat tggaaaggaa aatcccaact gttgacagtc acgatgtcta actccgtggg
    28261 cggttttaca aattttgacg actcagcgac cctagatgat ggaaaatttc atgtaacgat
    28321 cgtaccttcc ttagatattt tccgatttat tttttatttg cctagaatca tgagaggcaa
    28381 attctatacg atccccggca ttacttattt cacagccaaa cgcatgaaaa tccaagcgca
    28441 ggaaaaatct gtgggcactc gaacggatgg agacacgaca gatgatcttc cagttgaatt
    28501 atctgttgta tcaaaaggat taacagttta ttcaccaaag aaagaagaaa aagcttagaa
    28561 atagtagtta acagaaagtt ggaagaagag taatggatgt attgttacac aatcctttgt
    28621 ggttttacct tttcattgtg aatttttact tattttgtct gatggggtat gataaacatc
    28681 aggctagaac tagcggctgg cgtgttcctg agaataattt gatatttgtc agcctgttag
    28741 gcggtggttt gggaggattg atctctcgaa aagttttcca tcataaaact cggaaaaaga
    28801 agtttactat aggattcgct gcaggtgtac ttgttgattt gattctcatt tattttttcc
    28861 attaaggagc aggagatgtc gtctaaagga acgaaaacta aaatcattct gaatctgctt
    28921 ttgctgattg tgatttttgc cattatttat tatttaatca atcaatcatt tgcagatatc
    28981 tttgccgaat tgctgtctac cagtgttgtt gttttggcag cggctgttat attagggaca
    29041 gtttaccaaa tcgctgaagg gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat
    29101 tttacaacga aagatgggtt cttgacctct agctatattg cgttttaccg aatcgtttcg
    29161 tttggtacag gaacgctgat ctcggagatt tatttttata acaaaaaagg aatgaagcta
    29221 tcccagggag caggggtgac agcactccat atgattatgt acaaactggc agtgatcact
    29281 tatgcattag ctggcttggt catccaattc tctctttttt attctaatgg tccatcaatg
    29341 atctggttta tcatcgctgg tatccttttg acgatcgtga ttgttgcctt tttattggcg
    29401 atgtccgtca gcttgaatgt tcaaatattt tttgtcagct tatcgaataa gttatttaaa
    29461 agtgagcgtt tgcggaaatg ggtggataca tgcaatcagc agatctactc tttaagggaa
    29521 acggtccaaa ccattatcca agacaggact gcgctattgc ggatttatct atggaatatg
    29581 ttaaaacttg tattttggta tgtacttcct tatttgattc tgattgaaaa ccatcctaaa
    29641 atagattttc tactgaccat ttcgtttatc agctttgcag tagtgctttc aggggttatt
    29701 cctacgcctg ctggtattgg ttcctttgaa tttgtctatt tgttattatt cagaccagtt
    29761 gttggaacag ttgacgcagt ttcttcgctt ttactgtatc gtttcagttc ctttatcttg
    29821 ccatttctct atggatttgt gtatgtgatt gctgaacggc gaagagtgat caaagaagaa
    29881 ataatagaag taaaaaaaga gaataaagaa aatcaagggt aggtctttta taagatctac
    29941 cctttttatc aaatcaaaga aaaacgttat ggataagata attttacgtt tgtttggctc
    30001 tttttttata tacatacaat caattttgtt tttttaagat ttatataagt cgagaaaaaa
    30061 agtcaaaaaa ctatgtgtat attttgacca aaattgacca aagcgatata atagtttttg
    30121 taaagatcaa agaaataagt gatcttacct ttaattagga gggtatacct gtatgaatat
    30181 tgagaaaatg actacgacgc ttcaagaagc gattgctgaa gctcaacaga ttgcggtcac
    30241 tcgaaaacat caagatatcg acattgccca tgtatggaaa atctttttac aatctaatca
    30301 ttttggtcga aatttctata cggatgccgg attggatgtc gaatcttttg aacatgaaat
    30361 tgatcgtcta ctggatgagt atccagttgt ttctggtgga aacgtccaat atggacaaaa
    30421 cctcagccag aatttctttc gattattgaa tgaagcagac caaatacgtg aaagcttcgg
    30481 cgacgagttt ctttcaacgg aagtcgttat tttagcgttg atgaaattaa aaaattatcc
    30541 gttaactgtt tatttgaata aaaacggatt atctgaaaaa gaattgcgta aaaacatcga
    30601 agagatgaga ggaggagaaa gagtgacctc acaaaaccaa gaagaacaat acaaagcatt
    30661 agaaaaatac ggcgttgatt tagtacagca agtcaaaagc ggaaaaatgg atccaatcat
    30721 cggtcgtgat gaagaaatcc gtgatgtcat ccgtattctt tcacgaaaaa caaaaaataa
    30781 tcctgttctg ataggagaac ccggtgtagg gaaaacggca atcgttgaag ggcttgctca
    30841 acgtatcgta cgcaaagatg tgcctgaaaa tttaaaagat aaaacgattt tttcattaga
    30901 tatgggtgca ttgatcgctg gagcaaaatt ccgtggtgaa tttgaagaaa gactaaaagc
    30961 agtcttgaaa gaagtcaaaa aaagtgatgg acgtatcctc ttgttcatcg atgaaatcca
    31021 caatatcgta ggggcaggaa aaacagaagg aagtatggat gccggaaatc tgttgaaacc
    31081 aatgcttgct cgcggtgaat tacatttgat cggtgccaca actttagatg agtaccgtca
    31141 atatatggaa aaagacaaag cgttggaacg tcgctttcaa aaggtattag tcaaagaacc
    31201 aacagtggaa gatacgatca gtattctgcg tggattaaaa gaacgttttg agatccacca
    31261 tggtgttaac atccatgaca acgcattagt agcagcagct actttatctg accgttatat
    31321 tactgatcgg ttcttaccgg acaaagcaat cgatttgatc gatgaagcca gtgcaacgat
    31381 tcgggtagaa atgaactcta tgccaacgga attagatcaa gttactagac gtttgatgca
    31441 gttggaaatc gaagaagctg cactgaaaaa agaatcagat gatgcaagta aaaaacgctt
    31501 gaaaaatttg caagaagaat tagctgaact tcgtgaagaa gcaaatgcga tgaagatgca
    31561 gtgggaaaca gaaaaagaag aagtcaattc tgtttcagca aaacgtgctg aaatcgataa
    31621 agcaaaacac gaattagaag atgcagaaaa taactatgat ttagaacgcg ctgctgtatt
    31681 gcgtcatggt acgatacctc aattagaaaa agaattgaag gaattagaag ctaaagcaaa
    31741 agacagcgag attaagatgg ttcaagagtc cgttaccgaa aatgagattg ctcaagtcgt
    31801 tggcagatta acgggtatcc ccgtaacgaa acttgtagaa ggtgaacgag aaaaactaat
    31861 taaattgaat gaaacacttc ataaacgagt gattggtcaa gatgaagcag ttgatgcggt
    31921 cagtgacgcg gtaattcgtt cacgggctgg attgcaagac ccaaatcgtc ctctaggttc
    31981 tttccttttc ctaggaccaa ctggtgtcgg aaaaaccgaa ttagcgaaag cgttagctga
    32041 gaacttgttt gactcagaag atcatatggt acggatcgat atgagtgaat atatggaaaa
    32101 acatgctgtg tctcgtctag tcggagctcc tccaggctat gtaggttacg aagaaggtgg
    32161 tcaattaacg gaagctgtca gacgaaatcc ttatacaatc gtcttgttgg atgaaatcga
    32221 aaaagcgcat cccgatgtct tcaatatctt gttacaagtc ctagatgacg gacgcttgac
    32281 tgattcaaaa gggcgagtag tagatttcaa aaatacggtt ctgatcatga ccagcaatat
    32341 cggttcacag ttactgctgg aaggcgtgac tgctgatgga acgattccag aagcagtggc
    32401 tgaacaagtc aacacgctat tacgtggaaa cttcaaacca gaattcttga accgaatcga
    32461 cgacacgatt ttatttacac cattgagcct agacaacgtg aaaggaatcg ttgacaaaat
    32521 ggtggcgcaa ttggcacaac gtctggaaca tcaagaaatc ctgctgacaa tcagtgatga
    32581 agccaaaacg tggattgctg aaaatgctta tgaaccagca tatggtgcaa gaccattgaa
    32641 acgatttatc actaaggaag tagaaacacc actagctaaa gaaatcgttg caggacatgt
    32701 tatgcctaaa tcaaaagtaa cgattacttt acttgatgga caacttcatt tcaaaacaga
    32761 agagctagaa gaaatcgtct aatcttccat tgagactata aaagaaaaaa ggggtctgac
    32821 acaactagtg tcagacctcc ttttttcaaa taaacagtgt tccagaagta gtttcttcga
    32881 aaaataagcc gaaatttctt gaagctgccc gcttctgtca caacctcttt tattaggtat
    32941 ttttcacttt ttctaattta taactttcaa agtaacgctc atctttttta ctttgagacc
    33001 atgcaaacaa tcccattaag gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat
    33061 ccatcaatgc tgctcgtccg acaaacagtg tgccatcaga agcagcaaaa cgttgaagat
    33121 tcatgcctaa gaactggtcg cataagaatt cccagaactc ccaaaacacg ccgcaaagtc
    33181 cagcaaacgc aaagccgaat aagaggaaca accaaggact ggtttttgat atctcagcat
    33241 ctttcatcaa gtaaccaatc aatccatagc caagagctgt caggaccatt ggactcaccg
    33301 catgtaagat tttgtcccaa aaagagatga tactgatcaa atgcatccct gtgcctaaaa
    33361 agacagagat gaacaagaaa aaccagtaaa acaaaacgat tgctgctggt attttaagtt
    33421 tgaaaacttt caaaatgact tgtggcatga aaatcaatgc aatccctaat aaagattcca
    33481 cgataaatgt gattccttga gaagtcgaat atttttgtgt gataaattca taaaaattat
    33541 agcagacaac gaatacacca aaagcaagta aagctatttg cacctttttg atttctttaa
    33601 tcactgaaag tccctccatc taaaaatctt tttacttctg aaagcaactc aatttctgaa
    33661 gaaaccactt ttccgttcaa tttgatcaaa cctattgtat ataagttcag gtagtgaaat
    33721 tgattttcag ctgtttcttg tagtgcagcg atttttttcg ggtgggttcc gccttgctgt
    33781 cgagtatcag tatataagcc gatcactggg atgcctttcg cataagccac accgatttcc
    33841 gatgctaccc cggcatcgat cgtcagccca tctagtaaag caatcattaa atcactagcc
    33901 aacacttttt ctgtgtcagc aagagcaatc attcgactgt ctgcataagc agttttgtca
    33961 ttgattgctt cattttcttg aggaagatag atcgaaagct cagaagagag ctctctgatt
    34021 tctttaacta actgttggtt atataacaaa tcacttttag caaacattgg tgcggcaaaa
    34081 taaattttca tctaaacatc atccttttta tcaaaaataa tcgctaaagt aagcttccat
    34141 gtttggaatg atacgctcta gtgacttcag tgtttcttca tctgtttcta accgttcgat
    34201 tcttgccaaa taagaagcac ggcgataatt catcatcaag caagtcaatg tttggatatc
    34261 cagtcgaacg gattttccta taggttcatt taagacacgg acttgtccct gttcatccca
    34321 tgttaaagcg aagacaccat tattccaagg agcgacagga tcttcaatga tgaaatgaaa
    34381 agcatcagct gttccaacaa acggaaaccg ttgaaggaat tcttttacat caacgatccg
    34441 agccataaaa tatggttcga tctgctcttt gatctgacta tcttcaatca aaaatgctaa
    34501 aggctcattt ttataaatat ctccatgtac ccagtagatc atggaaaaat gtgcggaaat
    34561 aaagttccat aagccatttc gagcttcttg atcaagatag aacatttctt ttacgtgaaa
    34621 tatttcttca gctacccagt agaacagaac acctcttggt tcatggttgc tatcgtagta
    34681 gacagcggct gtgcgctcat cttcattttc gaaacgccaa tactcttccc aatgaaaatc
    34741 actgcggaat aaagcaccat ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc
    34801 tggatgatca actggtaatc gctcaaccat accacttact gggatttgtt taggtagctg
    34861 tgtatctctg attttaaagg aaagtttatc agacatgatt tcccatcctt tacgccgata
    34921 gtaggggata ctgtaaggat acaaataaga gatccattgc ttattcttgc gcatattatc
    34981 caatgcaacg ataattagat cttgcatcaa tccatggtta gcatattctg gataagttcc
    35041 aactccagtc acaccaccca ttttataacg tgcaccgtga atattgactt cacaaggata
    35101 gatagcgatt tgagagatca gtttattttc gtggaaccat ccgaacactt tcgatacttc
    35161 caaaatcggt tttttggaac ggataaaagc tcgtttattc tcaaacccgc tttcctcgat
    35221 gtctgcttca gtcacttgga acacatagga aagcaattca ttgaattgat cgatatgttc
    35281 ttgagtgacc ggttttaatt caagattttc tctaaaatct ttttgatcca ttttttcatc
    35341 tccatttatt agcttgataa cagtatagca aaattctaca aaaaaaactt ctatagctga
    35401 ctaattaaaa actctttaaa ttactgcttt tcgttggtat aatggaaagt gctgattata
    35461 tgaaaagagg agacgctatg gatataaaga aaatgaaaga acgccaagag cagattcgta
    35521 atttttcgat catcgctcat atcgaccatg ggaaatctac attggccgat cgtattttag
    35581 aaatgacgca cactgtgacg tctcgcgaaa tgcaagatca gctgttagac tcgatggatt
    35641 tggaacgtga acgtggaatc acgattaaat taaatgctgt agaactcaat tacacagcga
    35701 aaaatggaga aacatatatc tttcacttga ttgacacccc agggcacgtc gacttcacct
    35761 atgaagtctc tcgaagcctt gctgcctgtg agggtgccgt tttagtggtc gatgcagcac
    35821 aaggaatcga agcacaaaca ttggccaatg tttatttagc gttagacaat gatttggaga
    35881 tcctgccagt catcaataaa atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag
    35941 aaatcgaaga cgtaatcgga atcgatgcta gtgaagctgt attagccagt gccaaatcag
    36001 ggattgggat tgaagatatt ctcgagcaga tcgtggaata tgttcctgca ccaagtggag
    36061 acttagatgc accgctaaaa gctttgatct ttgactctat ttatgatagt taccgcggtg
    36121 ttgttctaaa tgtccggatc acggacggta tggtcaaacc cggagacaaa atcaaactga
    36181 tgagtaatgg caaaacattt gatgttacag aggtcggggt cttctcgcca aaagcagttg
    36241 ctagagactt tttgatggtc ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc
    36301 aagatacacg agtaggggat acagtaacgt tagcagacaa tcctgccgct gaagctttac
    36361 caggttaccg aaaaatgaat ccgatggttt attgtggtct ttatccaatt gatacttctc
    36421 gttacaacga tttgcgggaa gctttagaaa aactgcaatt gaatgatgct gctttacaat
    36481 tcgaaccaga aacctctcaa gcattaggtt ttgggttccg ctgtggattc ctcggacttc
    36541 ttcatatgga tgtcgtccaa gaacgcttgg aacgggaatt caatctagaa ttgatcacga
    36601 ctgcaccatc cgttatttac catgttaata aaacggatgg tacgactgct acagtagata
    36661 acccggcaga ttttcctgaa cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag
    36721 cacagatcat ggtcccaaat gattttgtcg gtgcagtaat ggaactttct caacgaaaac
    36781 ggggagaatt cattacgatg gattatttag atgattaccg agtaaatgtc gtctataata
    36841 ttccgctttc tgaaattgtc tttgactttt tcgacaaatt gaaatcaagc acgaaaggct
    36901 atgcctcatt ggattatgaa atgtctggtt atcaaaaaag caagttagtg aagatggaca
    36961 tcttgctgaa tggagaaaaa gtcgatgcat taagctttat cgttcaccga gactttgcct
    37021 atgaacgtgg aaaagcaatc gttgaaaaac tgaaaaaact aatcccacgc caacaatttg
    37081 aggtgccaat ccaagcagct attggacaaa aaatcgttgc ccgttcagac atcaaagcct
    37141 tgcgtaaaaa cgtcttggct aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct
    37201 tagagaaaca aaaagaaggg aagaagcgga tgaaacaaat cggctccgtc gaagttccgc
    37261 aagaagcctt catggccgtt ctgaaaatgg acgaggacga accgaagaaa tagcaagggt
    37321 ttgtttgaaa acagtgtaaa aaataaacca tgtttacatg gatttgccac cggtttgcca
    37381 ccggtggttt tt
//
LOCUS       DANKMN010000019        36947 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000019 DANKMN010000000
VERSION     DANKMN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            44..1411
                     /gene="nhaC"
                     /locus_tag="PW224_000957"
     CDS             44..1411
                     /gene="nhaC"
                     /locus_tag="PW224_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2907664.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1692..2711)
                     /locus_tag="PW224_000958"
     CDS             complement(1692..2711)
                     /locus_tag="PW224_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2907665.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2733..3740)
                     /locus_tag="PW224_000959"
     CDS             complement(2733..3740)
                     /locus_tag="PW224_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2907666.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="PW224_000960"
     CDS             complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="PW224_000960"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2907667.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4391..6685)
                     /locus_tag="PW224_000961"
     CDS             complement(4391..6685)
                     /locus_tag="PW224_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2907668.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6945..9110
                     /locus_tag="PW224_000962"
     CDS             6945..9110
                     /locus_tag="PW224_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2907669.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9414..10247
                     /locus_tag="PW224_000963"
     CDS             9414..10247
                     /locus_tag="PW224_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL2907670.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10349..12049)
                     /locus_tag="PW224_000964"
     CDS             complement(10349..12049)
                     /locus_tag="PW224_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907671.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12556..13716
                     /locus_tag="PW224_000965"
     CDS             12556..13716
                     /locus_tag="PW224_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2907672.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13732..14175
                     /locus_tag="PW224_000966"
     CDS             13732..14175
                     /locus_tag="PW224_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2907673.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14658..15188)
                     /locus_tag="PW224_000967"
     CDS             complement(14658..15188)
                     /locus_tag="PW224_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL2907674.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15293..15844
                     /locus_tag="PW224_000968"
     CDS             15293..15844
                     /locus_tag="PW224_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2907675.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15997..16470)
                     /locus_tag="PW224_000969"
     CDS             complement(15997..16470)
                     /locus_tag="PW224_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2907676.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16608..18080
                     /locus_tag="PW224_000970"
     CDS             16608..18080
                     /locus_tag="PW224_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2907677.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18077..18787
                     /locus_tag="PW224_000971"
     CDS             18077..18787
                     /locus_tag="PW224_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL2907678.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            19139..19423
                     /locus_tag="PW224_000972"
     CDS             19139..19423
                     /locus_tag="PW224_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL2907679.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(19740..20123)
                     /locus_tag="PW224_000973"
     CDS             complement(19740..20123)
                     /locus_tag="PW224_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL2907680.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(20125..20709)
                     /locus_tag="PW224_000974"
     CDS             complement(20125..20709)
                     /locus_tag="PW224_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL2907681.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(20756..22231)
                     /locus_tag="PW224_000975"
     CDS             complement(20756..22231)
                     /locus_tag="PW224_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL2907682.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            22412..22723
                     /locus_tag="PW224_000976"
     CDS             22412..22723
                     /locus_tag="PW224_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907683.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(22838..25174)
                     /locus_tag="PW224_000977"
     CDS             complement(22838..25174)
                     /locus_tag="PW224_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2907684.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            25316..25984
                     /locus_tag="PW224_000978"
     CDS             25316..25984
                     /locus_tag="PW224_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2907685.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            25968..27029
                     /locus_tag="PW224_000979"
     CDS             25968..27029
                     /locus_tag="PW224_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2907686.1"
                     /translation="MRRLFKDEKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRH
                     SISPAESAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFS
                     IRMDEQQKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLL
                     QAPNLSDEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREV
                     IVFFQPKWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFE
                     TATEIVLEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRI
                     DYSSIEGVGTTAMIRLSKQ"
     gene            complement(27266..28147)
                     /locus_tag="PW224_000980"
     CDS             complement(27266..28147)
                     /locus_tag="PW224_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2907687.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            28306..29034
                     /locus_tag="PW224_000981"
     CDS             28306..29034
                     /locus_tag="PW224_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL2907688.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            29190..29507
                     /locus_tag="PW224_000982"
     CDS             29190..29507
                     /locus_tag="PW224_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2907689.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            29500..30108
                     /locus_tag="PW224_000983"
     CDS             29500..30108
                     /locus_tag="PW224_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL2907690.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            30163..30954
                     /locus_tag="PW224_000984"
     CDS             30163..30954
                     /locus_tag="PW224_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL2907691.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            31092..31580
                     /locus_tag="PW224_000985"
     CDS             31092..31580
                     /locus_tag="PW224_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2907692.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            31631..32503
                     /locus_tag="PW224_000986"
     CDS             31631..32503
                     /locus_tag="PW224_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2907693.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            32679..33041
                     /locus_tag="PW224_000987"
     CDS             32679..33041
                     /locus_tag="PW224_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2907694.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            33050..33802
                     /locus_tag="PW224_000988"
     CDS             33050..33802
                     /locus_tag="PW224_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907695.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            33816..35618
                     /locus_tag="PW224_000989"
     CDS             33816..35618
                     /locus_tag="PW224_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907696.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            35895..36935
                     /locus_tag="PW224_000990"
     CDS             35895..36935
                     /locus_tag="PW224_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2907697.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT        11455 a         6547 c         7633 g        11312 t
ORIGIN      
        1 tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata aaaatatttc
       61 tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag tactgatcat
      121 cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt tgttgtttta
      181 tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca aaggaatcac
      241 tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg cttcatggat
      301 tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt ctgtgcgttt
      361 ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg gcagttcatt
      421 tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac ttggattcaa
      481 tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata actgctcgcc
      541 tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat tcgaacatat
      601 cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt tttatacgat
      661 attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga tccaaaacat
      721 gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt tatttggggg
      781 ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag ctgctctcgt
      841 tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca tcttgatgaa
      901 tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc gaggcgggat
      961 catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag gtgggttact
     1021 gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga tggaccgacc
     1081 ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga tcgttggaga
     1141 acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg atgacgttgg
     1201 cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg tcaattctct
     1261 cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta gcgcgttgca
     1321 ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat taggcggttt
     1381 tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct gggacagaag
     1441 cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaat ttttgtgaat
     1501 ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
     1561 agacaaaagt gttctttact tttgtcccac gctctttttt acagataaac aatgtgttag
     1621 aagtagtatc ttcttttaca taaaaagctg accataggat aaatggtcag cgtatttagt
     1681 gagtcagcta tttagtatgg gttttctaaa ggaacgactg tctctctttt gatcaagcga
     1741 tgagggatga ccaaacgtac gccactgctc aattcttcat ggatcaattc gcgcagcttt
     1801 tgcatagcga cacgtcctaa ttcggagata tcgatatcga tacttgtaag gtaagggtgt
     1861 gttaatgttg agaagatcga gttgttgaag ctaatgacgg agagtgtctc tggaatgaca
     1921 tagccataca tttgcgccaa ctgcatcgtg cgaagggcga agatatcgtc aatgaccacc
     1981 aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg acagataatc ctccggatgc
     2041 tctagtacta cactggataa cgcaggaaga ccagccatca tcattgcttt ttgataaccg
     2101 aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg tcacaaacag gatattctta
     2161 tgtccattct ggatcaaaaa ttctgttgct tgtttaccta gaagctggtt atcattatct
     2221 acataaacga tttcgttctc atgatgataa ggctgtccga tcaaagtgaa agggatgtgg
     2281 ttttggtata aataatcaat gacagtatct tgactatctg aatacactag aataaaacca
     2341 tctacttgtt tttgtaaatg catacgctgg acgttctcta acagcaattc aaaattttta
     2401 gcagtagcga ttgctgtagt catatcataa ttccgcgctt cttcgttgat tgcttccatg
     2461 atctcaagat aaaatgggtt accgatccgc tcttttgaat caagaggggg taagattacg
     2521 ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag tcatgttagg aacataacct
     2581 agttcatcca taatttttcg gactttcttc tttgttgtct cggaaatact aggatgatcg
     2641 ttgatgacac gagagacggt cgaggtggca acaccggctt ttttcgccac atcttttacg
     2701 gtaatagcca tgataaaaac acctttctct tgttattgtg cagagaaccg ataaatcgtc
     2761 tgactgatcc gttcttgacc aggatggagg ttgatcgatc caaatttggg gaaatgaacg
     2821 atatcaggga tttcttgcgg ttcgattgct aatccgtagt ttgaatgcat ctgttttcca
     2881 ttgacagaga aatcagcttc gaatccagtc gtagaaaata ataccatact tttattagaa
     2941 gttgctatag tcatttggcg accagaatgc ttctcgaata attgtaagga agttttggaa
     3001 agcctaggat gatgcaaaag aaatacatca tctagtccat ctggataaca tgcaaggatc
     3061 tcttgcagtg ttggggattt tctaaagtcg attggcagtt tttctttttt tgtccaattt
     3121 ccagtaggca gtttttcttg atctaattca agcacaccat ctaaaaagac cgttaactga
     3181 tgttcataga tgtctcttgt tccatttcct gaaaggttga agtaggcatg gttggtcgga
     3241 ttgcagatgg tttcaacaag gctactagca gaggttgtca tctctaattc gttttttgtt
     3301 aaacggtaag tgatcgtagc agtgattggt cctggatagg tagaaaattc atcttttaag
     3361 taaaagacca cacctattga tgatggagtt ttgaaaactt ctacttccca aaattggaaa
     3421 gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc agtttttctc caatctatgc
     3481 tcaccccaag aagcatcccg gatccttccg gctacaggtc cgacagttgc tccaaaaaag
     3541 cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca ataaaacatt ttctgatttt
     3601 ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac cgtaattgag aagaacgact
     3661 tctaagtagt cgttcttcaa tgtgattttc tttatttcag tctctggaac tggtacgacg
     3721 gagatctctg gattatccat ggtcatgcca cttttctttc aagtaatcga tcgtaagaaa
     3781 agtcgtatca gggacaagcg ggatctcctc tccaaggtct tcagcacgtc ccacgccaat
     3841 tggttgagcg ccacttgaga ggatggcttg gataccggct tgcgcgtctt cgatcccgat
     3901 acattcttct gcttttagtc cgacagcttt tgctgcaagg aggaagatat ctggcgcagg
     3961 ttttccgttt gttgcttctg ccgggtcggc aatagcatca aagtaaggtg taagacccat
     4021 tttttcaagt aggaatggcc cgtttttact tgcagaagca agagcaatct tgattttttc
     4081 ttcttttaaa gaatccagta aaggcacgac cccggggaag acatcttttg gttcgatcgt
     4141 ctggatcatc tctaaataat aatcgttttt tcttttcgct aattctgcaa attcttcctt
     4201 tgaaaaagag tgttcttttc cgccataagc taaaagcaaa gagagtgagt cttctcggct
     4261 gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta atgccgattt cctgaccaag
     4321 ctttcgccaa gcacgataat ggaattcagc agtatcggta atgacaccgt ctaaatcaaa
     4381 caatacccct ttaaacattt gcttcctcct ttcttagcgt tatttctaga cgatccgcta
     4441 aagaataaga ctgttcatag acgtaaattt ctagaggaga accttctaat aactcgaaag
     4501 taacttgttc atctgtgact gagatgaaca ataaacgtcc gcgataattg atatggaaag
     4561 aataagcttt ccaagttttt ggcagaaatg gtgcgaatct caactgatca tgggcagttt
     4621 tcatttgagc aaatccttgg acaatcgaca accagctacc agtcatggaa gtgatgtgca
     4681 agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa gcgagctgtt cgctggtaca
     4741 tttctacagc tttgctttcc attcctaatt ctgctgctaa aatcgaatgg atacttggcg
     4801 aaagggaaga ctcatggaca gtcattggtt catagaaatc aaagtttttt tgcttttctt
     4861 cttttgtaaa agtatcattg aagaaataaa tcccctgtag tacatctgcc tgcttgatga
     4921 agcaagatct aaggattttg tcccaagacc aatgctgatt cagaggaaga tcgcttgtgg
     4981 gaatatcgga tacaggcatg agatctttat ctaagaatgt gtcatgctgg acaaagattc
     5041 ctaattcttc gtcaactggg aagtacatgt tctctgcgat atctttccat ttggctagtt
     5101 cttcttcgct gatagtgata gcagcttctt tttgataagt taaataattt tctcgcgtgt
     5161 aacgcaagac ccaagctgcg atcgtatttg tataccaatt gttattgata ttgttctcat
     5221 attcgtttgg gccggtcaca ccgtggatca tgtatttatt tgctcgtttt gaaaaatgga
     5281 cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc cagtccttca tttttcaaat
     5341 attccgtatc gccggtgtaa ttcgtataat tgtagattgc ataggcaatc gcgccatttc
     5401 ggtgaatctc ctcgaatgtg atttcccact cgttatggca ttcgacacca gtgaaggtga
     5461 ccataggata aagcgcgcct tttaatcctt gttgctgagc gttgtggatt gcttgcggca
     5521 gttgattatg ccgatatttc agcagattct tagttacttc aggtttggct aaagcaagat
     5581 aaagagggac agcatacgct tctgtatccc agtaagtggc tccgccgtat ttttctcctg
     5641 tgaatccttt ggggccgata ttcaatcgtt catcttcgcc gtagtaagta gagaatagtt
     5701 ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc tccttcgatg accacatcgg
     5761 caagcttcca tcgttctgcc caagccgctg tttgctcttc ttttgcttgg atataattgt
     5821 ttgtctggtt cgttaattct gagacagctt tttctgcttg ctgcgcttct ggaatatctc
     5881 tactagtaag aacgataatt tctttttcca ctgaccatgt ctcaccttga gataattctg
     5941 cagacaagca accatgtaat gctaaagtat cttcgtgata gtcagggatg actgattttc
     6001 cattaacgaa atggcgcatt gctgcagtaa cagtaaattg ttcaatccca aagttgtttg
     6061 gaatagtttt agtcgtcaaa tagccgattt cttctatagt tccacgatca atctcattcc
     6121 aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt tccatccaat tttgatacga
     6181 gtttgactgt tccgctacct tcaagcattt ctatagttaa atagatataa gctgattctt
     6241 ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac tttattagta gaagtagtga
     6301 ttgtgaatgt acgagataat acaccatttt tcatatctaa ttcccagtaa aagtcttctg
     6361 gttccaaaga agccaagtcg atcagctgat cgttgatcga aatatctatc gcgataaagt
     6421 tcaaggcgtt gatcactttt ccaaagtatt ctggataacc atttttccac cagccgacac
     6481 gtgtcttatc gggataccag acacccgcca aataagtacc ttgatggtgg tcccctgagt
     6541 aagtttcttc aaagttcccg cgcatcccca tataaccgtt tccaatactt gttaaagatt
     6601 cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct gattttccat ggatcaatag
     6661 caaatagtcg tttgatctgt ttcatttttc tcctcctaat gagctgtttt atacgttcat
     6721 aataaacgca accgattgca caagtcaata gacaaaagaa aatatttcat agaaaaaagc
     6781 ttaaaaaaat aaatttttag gagaaaatta tagaagggaa cacacgtatt ataggtttta
     6841 tagaatggta tagacagaaa aataaaaaat aaagcattta caaaaaagaa aacgcacgct
     6901 ataatacgtt taacgaaacc gattgcgtaa aggagctaaa cggaatgaaa aagatattta
     6961 gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt tgtggtagca gttatgccgg
     7021 cagctgggtt gatgatcagt atagggaaat caattccctt gatcaatcct gattggacgc
     7081 cgcttgtcac aacgggcgga gtaattgaaa atattggttg ggcaattatt ggtaatcttc
     7141 atattttatt tgctttagca ataggaggaa gttgggcaaa agaaagagca ggcggagcat
     7201 ttgcagctgg gatctcattt attttaatca atcggatcac tggggctatc tttggtgtca
     7261 caagtgacat gcttgcgaat gaagatgcat ttacccacac tctgttcggt acgaagatca
     7321 tggttaaagg attttttaca agtgtattgg aagcaccagc tttgaatatg ggagtattcg
     7381 tagggatcat cgctggattt gtcggggcaa tggcttataa taagtactat aattatagaa
     7441 aattgccgga tgctttgtca tttttcaatg gaaagcgctt tgttccgttt gttgttatct
     7501 tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg gccaaacgta caggctggaa
     7561 tcaataattt tggtttatgg attgcagaat cacaagaaag cgcaccgatt ctagcaccgt
     7621 ttttatttgg aacattagaa cgtttactat tgccatttgg attgcatcat atgctgacga
     7681 ttcctatcaa ctatacacag cttggcggat cgtatgagat tttatcaggc gcacaggcgg
     7741 ggacacaagt atttgggcaa gatccgttat ggttggcttg ggccactgat ttagtgaatc
     7801 ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt gcataactgg acgccggctc
     7861 gtttcaaagt aggacaaatg attggttctt caggtatctt gatgggaatg gcttttgcga
     7921 tgtatcgtaa tgtcgatcct gataagaaag caagatataa atcaatgtat ttttcagcag
     7981 cgttggctgt ctttctaaca ggtgtaacag aaccattgga gttcatgttt atgtttgctg
     8041 ctgtgccatt atatgtcatt tattcagtga tccaaggggc tgcatttgcg atggcagata
     8101 tcttgccatt gcgggtccat tcatttggga atatcgaatt gctgactcgg acaccgttag
     8161 ctatcaaagc gggtctaggt ggagatctta tcaacttcgt gttgtgtgtg atcgcttttg
     8221 gtgttgttac gtatttctta gcaaatttct tgatcaaaaa attcaacttt gctacaccgg
     8281 gacgtaacgg aaattacgac aatgacagtg aagaaaccgt tgtatctaac agcggaacag
     8341 gaacagcaga tcagcaagtg gtccagatca ttcatttgct gggaggaaaa gaaaatatca
     8401 aagatgtcga tgcttgtatg actcggttac gcgtaagtgt aaatgatcga gaaaaagtag
     8461 ggactgaaga agcctggaaa cgagcagggg cgatgggatt gatcgtcaaa gacaatggag
     8521 tccaagcagt ttatggacca aaagcagatg tcttgaaatc agatatcgaa gatctgctgc
     8581 aatcaggagc agagattcca gagccagaaa tggtttctac aggaaatgga caagcaggta
     8641 gtaagcaata cttaggtata gaacaagaac tagtttccgc agcttctgga gaagtgattc
     8701 ctttatctga agtaaaagat ccggtattct cacaaaaaat gatgggtgac ggctttgctg
     8761 ttattccaac atcaagagaa gttgttgcgc cgatcgctgg aaaagtgaca agtattttcc
     8821 ctagcaaaca tgcaattggg ctggaaacga aagatggtat cgaagttttg atccatatgg
     8881 gaatcgatac agtccaaatg aatcagccag cttttgagat tgctgtcaaa gaaggccaag
     8941 aagtaggaac aggaacgaaa ttagctgaga tggatcttga tgtgatccaa aacgaagaaa
     9001 aagatccaac gatcatgatc gtatttacaa acgataaagt agaagaagta gtgatcaagc
     9061 aattaggtac agtgacagct ggtaaagtga tcggctccat taaaatgtaa ggagaggttg
     9121 tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc tcctcctatt
     9181 tttcgtttgg ttgggcttat taccgagaag cagcagtttg aatactgttt agtggagagg
     9241 tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc aaacaaaaaa tagctcctgg
     9301 tccattgagc ttattactga ggagttagca ggagaacact atttgctgga gaggttgtga
     9361 caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac aaggagaaaa ccaatgaaat
     9421 tcatgacact caacacgcat agctggcttg agccagaacc agagaaaaaa ttgcaagaat
     9481 tagcagataa gatcctgtta gaagattatg aagtgattgc tttacaagaa atcaaccaat
     9541 tactggaatc tgaagaagta gaacctgcga agctaatgaa gttctgtccg gtaaagaatc
     9601 aagtacctat ccgtaaagat aattttgcct accgtttagt tcagctttta aaggaacacg
     9661 gaaaagagta tttttggagt tgggaaatga gccacatcgg ttatgataaa tatgaagaag
     9721 gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt ccttaccgtc tcccaatttc
     9781 aaaagaaaga agattatcag acacgcaaga tcttgattgg gaaaacaaag atagatgatc
     9841 aggacatttt tgtctcgagc tgccactttt cttggtggac agataaaaaa agtggctttt
     9901 attttgagtg gaaaaacttg gaaaactact ttttggaaac tcgcgtacca ctttttttcc
     9961 taggtgattt caataatcca gtagactctc aaggttatta tactgtaaga gaaagttgcc
    10021 ttcttcttca agattcgtat gttgtcgcga atgaaaaagg aaaagcagct actgtggaaa
    10081 agaaaataga tggttgggaa caaaatacag aaaaattgcg tatagatttt attttcgtcc
    10141 ctgaaggaat gcaggttaaa aaatatcaac ggatttttga tgggatcgat agtccaatca
    10201 tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa cgaataaaaa atataacata
    10261 taacataaaa aaactgatta aaataaaact ctccaaacga ttataccgtt tggagagttt
    10321 tttgatcgga tgtatttatc ccttttgcct aagcacgtaa aagcttgtat ctatgataaa
    10381 taaataaaca tagcaaggta aaccagccaa tcaaagtaag agaaagaatg aaatagaaat
    10441 agattggcgt ttgataatga agcgtgagct cgttgattcc tttttgctgc ttcacgattg
    10501 gggtcccgat atctgttact tcataatcag tcaatgtttt tccattttgc tgtagcacag
    10561 tccgatcgta gacgataaca gggatatgaa cagttttgtc cccagtgttt ttccacttga
    10621 ttgtcaaaag accgcctgga tgtgttttta taaaagttgt ttcgttttct agtatttctt
    10681 cgttataccg gtcgtattta ttgtcttttg tctccttata gattggcaga tagtcaggtg
    10741 tggttttttg gaaaacgaac aagctggtag acatatcttt gctgaagaat gttttccttg
    10801 cttcttccgg cgtatcgaat agataagtat gtcgactgac aaatgtttca ttttcccact
    10861 tttccagatg ctggtataag ttctggatcg tctgtgctga aaaaataaga atgccaaata
    10921 tacttaaaag acggaaccac ttttgttcag accactggtt cagtgaaagg gcaaggtaaa
    10981 gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg aaactggatc aaactaataa
    11041 aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa gctagtagaa agaaaaaaga
    11101 aaacaagact tgtgtacaac acgatttttt ggaataaggt gttcttttgt tgaaagaaaa
    11161 taaaaagcaa aaaacctgat aatatcaata taggaaaggc cttagggtaa tggagccaat
    11221 aactgccatc cagatctatc gccttttgac tcatttctct gttaacaaag ggaggaagaa
    11281 tcttattccc actgtaaaca atagctaatc ctgcccaaat atttgctgtc agaacaaaat
    11341 aaagcaagac cgacttaatc agttgaagca gtgcttttcc tttttcagtc tggttaaaaa
    11401 agtaataaag ataaaaaggg atatacatca aagctaacat gatcgaagac agtaaatgga
    11461 tctgtgtcat aagtcccact gagacagcga ctttgataac agggaacttt ttatgaagga
    11521 cgaaatcagc aatagggatc atacataaag gataaaaaca agtcccccag ctagtgaatc
    11581 cttggttgat agaccagtat tgaatagaat acgtactcat gaataaaacg gctactggta
    11641 cacttaccca atttcgaaca ttgacatatc tcagtaaaat cagcatagaa aatcctgaaa
    11701 taagataaag aatgaaattt gaaagaatct gataatgaaa ccagctacct gcaagcaaca
    11761 cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac ggcattcacg atcctgccac
    11821 tttgctggaa tccataaagg gaaggaaaat aatggaaatc cccatattta atttgttgcg
    11881 cggcatcata gaaccgattg taatggaatg gggcatcagc acccaaaatt agtttatgtg
    11941 tgaataaatg aggcatgaga aatgccaaag caatgaaaaa aattgctaaa aatgggccat
    12001 attttttcaa ttgtcgaact cctttgcaaa ctttagataa attcatcata tatcctatgc
    12061 gagaggtagg caagaaatta gaaaattcat gaaaatattt atgaatttta caagaaaaac
    12121 ataagtgaaa gttctaagga gagaggatgc gacataagtt ttgttgcatc tccttgtttt
    12181 gaaacagaat ttttcttaat actcgaaaca aagtagcttt ttcatgacct ctcggtaaac
    12241 ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata tgagaagcta
    12301 ttttctgact agcgctctta ttactcaaag cataacgcag ttttcacaac ctcttcctta
    12361 aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa aatatgagta
    12421 gctatttttt tgggctgtac ttattacttg atgcaggaca gctttttcac aacctcatcg
    12481 gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca aaaaaacttg
    12541 caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact tttgtttcgt
    12601 ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc attttatgtg
    12661 gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa acggaccgca
    12721 gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt aactgaccaa
    12781 ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag tacaacatcc
    12841 agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca attaaatatt
    12901 tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt cttgcaagga
    12961 acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca cagcggttta
    13021 cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac gtttattttg
    13081 actgttttgg gggagaaatt ggcttatcaa gttaaaaata tacaagtggt actgccagat
    13141 gacacgtctg ttctaacgat ttcagaagga gaagacttag ttaccttgct tacatgtacg
    13201 ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc ttataacgaa
    13261 tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca atggtttatt
    13321 cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg actaatctat
    13381 cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga ttcgtcaggg
    13441 aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact tactagaaat
    13501 ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga tcaattgcct
    13561 ggtggaactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc ttttggtatt
    13621 agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt aaaagagctt
    13681 caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa aatgatccgc
    13741 acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg gctgcatgct
    13801 aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt agaatctgtt
    13861 aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga aattattggt
    13921 tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga ataccggcaa
    13981 caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac actgactttg
    14041 acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg ttttacaatt
    14101 caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa atggaatcaa
    14161 aacgctgatt tttgatgaac agaataacga aataatgctc ccagcctaat tttttgctgg
    14221 gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt tgttacaatc
    14281 tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
    14341 gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    14401 tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag cctagaaaat
    14461 atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct gatttcacaa
    14521 cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
    14581 catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc tgatttcaca
    14641 acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta ttttccgcta
    14701 cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc attgtctata
    14761 cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt tggataagtt
    14821 tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa agtaacagtg
    14881 atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag tttcttttca
    14941 tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt gattttgaat
    15001 acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg gcctttgacc
    15061 attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca ttcatattcc
    15121 caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa ttctttaaat
    15181 tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac atgttttcga
    15241 ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac tgatggcaag
    15301 aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga tgacaggata
    15361 tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa aacatagtca
    15421 gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc aagtagagat
    15481 cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga aggaacaatt
    15541 ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg aatttgttct
    15601 gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta ttcagcagca
    15661 gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa cgatctttcc
    15721 tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc atgcgatcaa
    15781 tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta accgaaaaaa
    15841 ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa acgcagatca
    15901 ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt agcatctact
    15961 tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta acaactgttc
    16021 gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg tctcgttgtc
    16081 atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta aaatcgtata
    16141 gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac ggacatggtg
    16201 cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa atccgtcatc
    16261 taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt ttctaggaca
    16321 attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc ttaattgacg
    16381 tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt gatcattttc
    16441 caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc tcttgcttcc
    16501 cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg aatttaggac
    16561 taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg agtaacaacc
    16621 aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt gtcttattaa
    16681 ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt cctacattga
    16741 tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt ttcttgctag
    16801 tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt tcaagaaatt
    16861 tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt acagcaccta
    16921 attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga gtatcgatgc
    16981 cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga tcagcaatgg
    17041 ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta tctggttata
    17101 tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc gttgttcttg
    17161 tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat cctagcattg
    17221 atattttatc cgctatttta tcaacgattg gattcggtag tttactttac ggattttcta
    17281 gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt gttggtgtga
    17341 ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc ttggaacttc
    17401 gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta acaaatatgg
    17461 cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt ggagaatcag
    17521 cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct atgatgcctg
    17581 tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca gggatgttca
    17641 ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca gttttatata
    17701 tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg ccggtaacga
    17761 cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg gtaaataata
    17821 cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg ttgacgaatg
    17881 tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta gcttataaag
    17941 atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta gcgatcatct
    18001 ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct gttctaatga
    18061 aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg cttttgcttt
    18121 aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt tcggactaat
    18181 cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg gaatggaaaa
    18241 agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag gagcagatat
    18301 gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc gaacaaatga
    18361 aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag tagaaaagac
    18421 agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa acgacatgta
    18481 taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag tgaatacatt
    18541 caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt tagctgaatt
    18601 agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc aagacggtat
    18661 ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac aacgtacgaa
    18721 agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag aagcaaaagg
    18781 cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa gctgctctgc atcaagcagt
    18841 aagaacaatc agataaaaat agcactccat atttttcatc tgattgggct tagtgccgta
    18901 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt taactctgag
    18961 tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgactattgt gacttgtgac
    19021 cgaggagttg gcaggagaac accgtttatt cgaaaaaagg aactgcgaca agacttttgt
    19081 cacagttcct tctttttttt gctatgctat actagatgta aaaaatagga gaaataaaat
    19141 gagggaaatt tattcttttg aatcaaaaat ctatgcttcc gaagtcggca agggcggggc
    19201 atatattatt tttccgtatg atataagaaa agaattcggt gctggaagag ttaaagtcca
    19261 agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg aatatgggcg tgaaaaatcc
    19321 agatggttca gtctgctaca tccttggact acgaaaagat attcgtaaaa acatcggaaa
    19381 agaaatcggt gatattgtag cagttacagt gaagcaaaaa taaaagaggt tgtgacaaaa
    19441 gacgtgtcac aacctcttga taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa
    19501 atttatgcaa aaatgtggaa acccatgttt acaaaatttt tcttactact cgaaacgaag
    19561 cggctttatc acaacctctt gataaacggc gttcaatcag ctgtccctcg gtattagctc
    19621 aaaaacggca aaatatgaga agctattttt ccattttttt ctctcatact caggtcataa
    19681 cgctgatttc acaacctctt ctttcataat ggtttttcac catctttata ataaactggt
    19741 tatttgatat ggaaatctat tttttccaac caatctaaaa tcttgtaacc taactcttct
    19801 gacatcggaa aagaggcgtt gatttgtgca ttggagatat ggtcaacatg gataccggaa
    19861 gtgatcgtac aacttcccgg aacggcagtt ttgatgatca cagctatttt ttcggctaag
    19921 acatcgtctt tatggaaccg accatcgtgg ctagggaaac gtacagtttg catctctgta
    19981 tcacgagaaa atgtagtgac tgttccgata tgtggtgtat ctccgcctgt taaagtgatc
    20041 aataagtctt taccgattac tttaacagca gcttggatcg tgaaacctag tttggttact
    20101 tcaactatcg tttctttttc catattaatc accctgccaa cgttttgaat gctcatttac
    20161 aatttcaaat tgatccaaaa gtttcattgt atggtgatta acaagatctt ggatcgtttg
    20221 aggctgatta taaaaagcag gaattggtgg tatgatttgc acacctagtc gtgctaattt
    20281 agtcaagttt tccaagtgaa tggcatttaa tggcgtttca cgaggaacaa tgactaactt
    20341 tcgttgctcc ttcattgtta catcagcagc acgtgcaatc aggttgtcgc ctaaaccaac
    20401 agagatacct gcgactgttt tcatacttgc cggtacgatg atcattccat cggtcaaaaa
    20461 tgatccgctg gcgacagcag cacctaaatc tttgtttgaa taatggtaat caaacaggct
    20521 ttcgaattca gttagtgata agtcggattc cagctttaag ttctgttttg cccatggact
    20581 gatgattccg tgtgtttcta tcatcggata ttcttttagt ttcttcacta ggttgatggc
    20641 ataaatggtg cctgaagcgc cacttacacc aatcacgatt tttttcttcc gttgtgccaa
    20701 ttgttccatt ttccgcactc ctttttatga tgtcaaaaca gttgcagatc attcattatt
    20761 tcaaatattt ttcccaatct tctacttctt ggaattttgc acgtacaaaa tgttctttca
    20821 tatcgaaagg aactgtaccg tcaaagatag tttttgcact tatcccacgg aaacgaatag
    20881 agccaggatt atattctggt aattctgatg ggtccaatgg atggttcctc attcctggga
    20941 tcacgatgat atctttatct ccttggaaac gagtgttgaa agtccaaact acatcgttca
    21001 tgtcgaaaat atcaacatct tcatcgacta aaatgactgt cttcaattct ttgaaggctg
    21061 ataaagcaag aatagctgct tgacgctgaa taccttcatc cgcttcgttt tctttacgga
    21121 tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg attgtagaca tttactactt
    21181 ttcctggaat agctttatcg accaaggcaa gaatacttgc ttctgtagga ataccggcca
    21241 tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat gattggattg tcttttcggt
    21301 gagtaactgc tttgactttc actacttgta cagctggatt agcatcccca gtatagccag
    21361 gaaattctgg cattgctttt cctgagttcg tgttgatatc ttcttgcatt gtttcatttg
    21421 gcatgatttc tgcttcaatg atgaattcag cacgagcaat acccacttca tcgactgcta
    21481 taccatcaac gatttgaata ggttcgttac gcaaagctcc tgcacaccat agttcattgt
    21541 agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat ggcaattgct ggatctaaac
    21601 caatattgat agtgataggc attggtttat tcaaggcttc gaattgtttt tggaagtgac
    21661 caatgtggcg tccgccaggc atgatgtaca taccgatcgt atctttatct tctagaacca
    21721 tacgatggat cgttacatcg ctcattgttt tttctggatc tgatcctaag actacaccca
    21781 ttgtgatgta tggaccagca tcttcaggag tatttgtagg tgcaggcaaa atgttgcgaa
    21841 tatcgaagtc tgcatcggta gctagatgaa cgacttcttg tgctggagcg tctgctttgt
    21901 ctactttcac aggtttgatt ggattttcta cagcgtcttt caagaaacga ccaagtgttc
    21961 gataatcatg gtgcaaaatt tcacctacac gtttacgact agccattgtt ccgatgttca
    22021 cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat agctggtcct tcttgagtcg
    22081 gacgttttac tgtaccgcca gcaccgatat aacggtaaac accagataat tctgcttcag
    22141 gatcgatttc aatatctgtt tcgtgatatt gacctggaag tgtttttagt tcttcgatga
    22201 cttttcttaa atcatacggt tgatctgtca tgagagattc ctccttagtt gtctttacct
    22261 atctatagta aaaggtgaaa aagaggaaaa caattcaact tgttcggtaa agtattctac
    22321 aatggaatgg atgcgttttt ttgataatag aataaactat gatatactta tcactaaaag
    22381 aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa atggaggagt catttcctaa
    22441 agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt acatttgaaa acggagaagt
    22501 aaaatacgtg aaatctcatt ggacagaaga gataaccgat gccttgcaat ttggaaaaaa
    22561 aggaagagga aaaagaaaaa atcttctcgc actttctcgt aatatgtgga ttgggactga
    22621 agtaacgatt gaagcagatg gaacagtatt cataaatgga aaagacaggt atacgccaga
    22681 agagttgtgg tacaaaggaa agaaaagtat accagaatta taaaaaataa ttaaaaaaaa
    22741 cagcgcgaaa caaggaacat attacttcct cgtttcttgc tgttttctac tatttcagcg
    22801 ctcttatgac tcaaatctaa atgtggttgt cacaacctta ttctgcacgc aaagcgatag
    22861 cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc gccaagcatc gtcaatatag
    22921 tagagacgat cactagaata atcgcatgga caggatttag ttgagcaaca ttttttagat
    22981 ccgtcatgtt gtataagatg ctattgatag ggaacgtagc tagccatgca ataaacacac
    23041 ctaatatccc agaagaaata cctaaaatac acgtttctgc gtcaaatacg cgagtgatgt
    23101 cttttttacg tgcacctaaa gcttttagta caccgatttc ttttgtccgt tcaatcacag
    23161 atgtataagt aatgatactg atcatgatca tacttgttac taaagagatc cctgcaaaag
    23221 caataaggac ataagtgata gcatccatca atccgccagt cagttcagtc atcgttccag
    23281 ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc tttattgtaa tcatctagat
    23341 aatccaagat cttttctttg tcttcaaaat tgttcggata aatcatgata cttgaaggca
    23401 agctgtctcc acctaaataa ctaagcaatg tttgtttggc attttcgtca actttttcag
    23461 tagttaagac atttacacca ctgtctcttt gagctttgac gatttctgat tctttgtttt
    23521 cgttgacgat ttgagtcgtt aattgatcgc tataagcgat tcctggagat aagagattca
    23581 ttgtagaaga agatttcacg cgtaggatcc ccgagatctt caattcatca gaagcattct
    23641 gatacatcgc atcataatct gtattaggga taaaattccc agttggaagt tttgtatagt
    23701 aagtattgtt gtttactaat ttgaatgttg taccaacgat atcatcaaaa tcaagttttt
    23761 cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt gatattagta ttattattgc
    23821 cgtctacgat cagcacgaca tctgttgctg aggctggata acttccggca agtaaagaat
    23881 aattgtcttt taaaaagttt tctttgctag tgtcaagctg tgtagggaaa cttgacactc
    23941 ccacacctgt cattgctgac atcgtactag ataaagaaag agattccgta tcaggatttt
    24001 gattcgagaa gctgacaggc tgtactttgc catttacatc acgtaaaaga ttgattcctg
    24061 tagtacgggt aaatccgata ttattactta agttaggatc gatgtctgtt acgtaatcga
    24121 cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc ttcttcgctg acttttgcag
    24181 taatcgtttt gctgtctgga taatccgctt tgcttgcacc caatccggca tcgtcacgag
    24241 tctggttagt cgttacctta gaaatagtaa tcggaaattt cgccatcgtt tcagcttgcg
    24301 tgttatcgat ttgtttttgg aatccagaag aaagggacag aacgatcgcg attccgatga
    24361 tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc tttttttgtt cggatattgt
    24421 tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt tctgcgtaag ttaaattgat
    24481 catcttttgg tcgttcgatg tgaggatggg aatctgttaa gatcttacca tcagaaaatt
    24541 caatgattcg atctgcgtat tgatgggcca gttcaggatt atgtgtgacc atgatcacta
    24601 atttttcttt cgataactct tggatcaatt ccatgatctg gatacttgtt tctgtatcta
    24661 acgctccggt tggttcatcg cacaaaagga tatctgggtc attggctagt gctcgagcga
    24721 tggccactcg ctgcatttgt ccaccagata actgatttgg ctttttatgc atatgatttg
    24781 tcaagccgac tcggtgcaat gcatcttctg cccgttttcg tttttcgtct ttagaaacac
    24841 cacttaatgt catcccaagt tcaacgtttt caataatgcc tagatgacca atcaagttgt
    24901 aactttgaaa aatgaaaccg atagagttgt ttcggtaggc atcccagtca ctgtccttaa
    24961 agtctttagt agactttccg ttgattacca tatcaccgga atcataatta tctagtccac
    25021 caatcacatt cagcatagtc gttttaccag aaccactagg acctaaaatc gctacgaatt
    25081 ctttttgtct gaaagcgaca gacacaccat ccaatgcttt ggtggtggtt tcgccgactt
    25141 tataatattt ttttatatct tttaattgaa gcataatcac ccttctcccg tattgaaata
    25201 ttcggtatac taataagtat atcagaacaa tgatcgtagt atacgaaata aatgttaact
    25261 gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa aacagaggga gatcaatgaa
    25321 tcgtatttta gtagtagaag atgatccgca attacagttg ctttaccgtt ctgtgttgga
    25381 acgagcgggc ttttcagtca tcaccgcatt gaatggagag gatgcgctga aaaaactaga
    25441 agcgacacgt atagaattaa tcattacaga tatcatgatg ccgaaaatgg atggctatga
    25501 cttgctagac tcattacgta gcaccagatt tgacacacct gttctaatga ttacggcaaa
    25561 ggccgatttt gaggataaga aacgcgggtt caaattagga gcagatgatt atatgacaaa
    25621 acccatcgat gtcaatgaga tgatcctacg tgtggaagca ttgcttcgac gttcaaagat
    25681 caattacagc agtagcttga taatcggaca aacaaaatta gagctagatg cttatacagt
    25741 gttacaagat cagcattcgt tgattctgcc tcaaaaagaa tttctgcttc tgtataaatt
    25801 actgtcttat cccaacaaga tcttcactcg ccaacagttg atggatgata tttggggatt
    25861 agatacgaat acagaagaac gaacgatcga tgtacatatc aaacgtttaa ggacccgttt
    25921 tgaaaagacg gaagattttc aaatcatcac agtacgggga ctaggctata aggcggttat
    25981 ttaaagatga aaaaaagcta ttttcacaat tatggatcta ttttgctagt attgtcttca
    26041 tttcgatctt agtgacactg ctgtgttttc tcggttttat tttcttattg tctcgacatt
    26101 ctatctcgcc tgctgaatcg gctggaaaac caacgttata cccgttattc gtctttgcgg
    26161 ggttcagtat ggtcgtgggt acaggaatat cgatttttgt tgggcggcgt atcctgcatc
    26221 caatcagtgc gctagggaca aatatgagtc tggtagcaac gggagatttt tcgattcgaa
    26281 tggatgaaca gcagaaagta gcagaagtac agcaactgta taaagatttc aacgttatgg
    26341 tccaagaact caatagtatc gagacactga ggaatgactt tgtgtccagt gtatcccatg
    26401 aattcaaaac accactagca acaatccaag gatatgttca attgttacaa gccccaaatc
    26461 tctctgacga agaacgccaa atatttttat atcggattat cgaaagtatc acgcagcttt
    26521 cacaattaac agagaacatc ttaaaattga acaaactgga aaaccagcgt atccaactgg
    26581 aaaagaaaga gtatcgatta gatgaacaaa tcagagaagt aatcgttttt tttcagccta
    26641 aatgggagaa agaacagcta gaattggata tcgaacttgc tgcagtaaat tacacaggca
    26701 atgaagagtt tttgtaccaa gtgtggctga atatcatgga taacgccata aagtacaatc
    26761 aaatcaatgg acagattcat atcaaactat ttgagacagc cacagagatt gtactggaag
    26821 tgaccgattc aggtgtagga atgaatgaag agaccagaga tcgaatgttt gaaaaatttt
    26881 atcaaggaga tacaagtcgt caaatttctg ggaatgggct aggactttca ctggttaaaa
    26941 aaattctaga actgcatgat ggacgtattg attatagcag tatcgaagga gtaggaacga
    27001 cagcaatgat acggttaagt aaacaatagg aaaaagaata aaacccctaa aaattctatt
    27061 gaaggttttt aggggttttt agtcgttttt ttgtgattag ggaaatgttc ttacggttct
    27121 tttggcataa aaatcgctgc tttcttattg aactacgaga aatgttagag gtgtcattga
    27181 aacaaggaca tgatgcaaga aagagagcga aagtcatgcg tcgctctctt tttttgggtg
    27241 gatagatgga cagaaaatgt agggcttagt tttcttcttg atttaaatac tcccaaaatg
    27301 tatctgaagc caaagaatgc tcaccgactt tacgagtaaa tgcaagataa ctctctgttg
    27361 ttggagaaat agggagaatc acagtatttt cttttaatag attcttgact gttttttcca
    27421 taacaatagc aatccccatg tcatttgcaa tcatattgat gatcaagtcg attcttgctc
    27481 ctttataaga gatttttggt tcaaatccag cttcgcgaca cagatctagt acaggttgat
    27541 acaagttcgt agtggggcca aggagcaaaa agttctcctt tttcagttcg cttagatcca
    27601 atttcttttt actagctaaa ggatgatttt taggtagaac tgctacaaaa tcgtctgttt
    27661 cagtaggcaa ccactcaatc gaacttggtg gggcctggaa acttctagca aagtggatcg
    27721 tgtcactatg ttcattggcg tcattgtaag ttaattcgac actttccact tctttcaact
    27781 ggactgtaaa ttcaggatgg cgtttcaaaa actcggtgat tttggaaaaa cctttataat
    27841 tgatcattgt cggtatcgtt aagatattta atgaaagatt gttgtctttc gtataggttt
    27901 caagcacttc ttgcatagca tggtattgtt ctacgacatt ttttacatat gggagcaata
    27961 agcttccagc ttcggtcaat tcgatttttc tatgggcgcg ttcgaataat ttgacgtcta
    28021 attctgtttc taacgaaaga atctgtttag aaatatttcc ttgggtggtg aattgtcgat
    28081 cagcagtttt ggtgaaattc attgtttcgg ctaaatcaag aaaaacttcg aatttatgga
    28141 tatccatcat attcctccta cttattccaa taaagaatac tttaacacaa aaaatgaatt
    28201 ggtgtaatct tctctgtctg ttacaatctt tcttgcagcg aaagcaataa ttacagaaat
    28261 ttttctgatt tttttcgctt cgtagaaaaa gagaggagtt ttttgatgcc aactggagtt
    28321 ttgatcaatg taggttccgt tttgtttggc ggactaattg gcggattagt aggaaataaa
    28381 ttaagtgaac attttaaagc acagctgacg atggtttttg gtgtgtgttc gatggggatg
    28441 ggaatctatt ccattgcgcc gatgaaaaat atgccggctg ttatttttgc tttagtgata
    28501 ggaacagcga ttggtttgat tgttcattta ggtaatggga taaataaggg ggctgcgctg
    28561 atgcaagtac ctatttctaa aatcttccct agtgaaaagc ttggtatgac ccacgatgaa
    28621 tttatcagca cattagtgac ggtcattgtt ctattttgtg caagtggtac ggggatctat
    28681 ggaagtttag attctgggat gacaggagac agtacgattt tgatttctaa atctgtcctt
    28741 gattttttca cagcagctat ttttgcgtgt aatttagggt atgtcgtttc tgtgatagca
    28801 attccccaat tcattatttt ttatattttg ttcttattgg caaaatttat ttatccattg
    28861 actacaccag acatgatctt agattttaaa gcttgcggcg gattcttgat ggtcgctacc
    28921 ggttttagaa tgatcaacgt gaaaatgttc ccagtagctg acatgatacc tgcaatgatc
    28981 gttatcatgc cactgagctg gatgtggaca aattggatta tgccactgtt ataataaaaa
    29041 tcaaagagaa gccatttttt gctgactaaa aaggatcaaa tcttttagtc agtttttttg
    29101 tatttgacaa actgattttg aaactatatt atatgttgta taatatagat tgaaataaaa
    29161 tattacactt gagaatagaa ggtgacgtaa tgcacatcca gattccaact gttcttcttg
    29221 atggtactgt gctagcagca ttaagcaagg aagatatgta tgggtatgcc ttaacaaaaa
    29281 tcgtacaaga aagtctctct gtcagtgaat ctacactata tccggttttg agaagattga
    29341 aaaaagaagg atatcttgag acctatgatg aaccttttga aggcagaaac cgtcgatatt
    29401 actcgttgac accaatggga agtcgtcatt tagaagaaat tattgatgaa tggattgttt
    29461 ttcgagattc tttagaccgg ttattggagg agagcacaaa tgaatgaata tttgaatgaa
    29521 gttgtggatc atcttcaaga aattcctgaa gacgatcgtt ttgatttgat ccagtattat
    29581 gaggaatatt ttttagattc tggaaaaaca ttagatcaga tcattgatga atatggtaca
    29641 ccaaagcagt ttgccttaaa attaaaaatc agttactttt cagaagaaga acaagcagaa
    29701 aacgagttag tgaaaaatcc aaaaagacaa attcgcctga tttggttaat cattatcggt
    29761 ttatttgctt ctcctgtcct cattccacta gcactggggt ttatattggt tattggggga
    29821 ctattggttg ctttactagc gctcattttt agtatctatg ttttatgtat cagtatctta
    29881 ggtgttggcc tattcatgat catctcagga atcagtgtgt ttggacaatc gattctgagt
    29941 ggattattct ttatcggtgt cggtctgttc gccacaggaa gcgcagtatt tatttcgcca
    30001 ctgttgctga aaggaacaaa agggcttttt tatgtcatga tgaactttgt taaatggatg
    30061 ggacgacgtt ttattacaaa aagagatctg cattcagcgg gcgtttgaag gaggacacat
    30121 aaattgagat tgaaacattt tttgattacc ggtattggtc tgatgcttac tggtggcctc
    30181 ctagcaggtg caacttatac attaggcgct caaaagagtc tgacttggga taatggtcct
    30241 cggttgatcg atatggttac tgagacaaaa aaaatcaagg atgcaaacaa aatcgtcatc
    30301 catgcagaaa atcaaacagt ttcggtcata cgcggattgg attttgagat aaaaacgact
    30361 tatgataaga cagaaaagcc agaaataaag gagaaaaatg gcgtgttgac tgtgaatgca
    30421 gtcaaaaaat caacaggtgc aatcattagt ttagaagaca caagtgcaca aatcactatt
    30481 gttgtccctg cagatcatga attatctgaa ttgacaatcg aagggaataa tagtgcaatt
    30541 gatatcaacg aagtatcaat caaaaaagtg aatactaaga cacatgatgg ctgggtatcg
    30601 ttcaatgaat ttacaggagg aacaataact gcgaaagatg aattaggctc acttcgatta
    30661 gatgaggcca atgtggataa cttgatcgtc acaggtgaaa acagcaactt ccatctagaa
    30721 gacagtttga tcagcaaaga aggaaaagtc atcctaaacg gtggcaattt atctttgcat
    30781 gagatggaaa acagtggcta tactatccaa acgtctggtg aatcttatat tgaaaaagac
    30841 taccagccaa taacaaatac tttgttagaa caaggacaga aaaagctaaa gatcactgct
    30901 aaagacacat cgatcaatat tacgacggat caagaggaca atgaaacaga ttgatcctgt
    30961 actcaactgg atattttaag aaaaaacagc aaagatcctt ccaatctttg ctgtttttca
    31021 tcgcttaacg cacataacta tagtaaaata agaaagaaaa tatctaaaaa gaaaacaggg
    31081 tgaaagagta tatgaaggta gaagaaggtc aaagagaaaa gttaaaaaca gaaatggaaa
    31141 gattacatac ttatatcact caactaagcc aaaccttcta tgatccagac aaagaaaaag
    31201 tgatggttaa ttacccgaac aattcagaag gtcgccaatt agagcaagtg taccatgaag
    31261 ttttcaaaca tttattgacg gtgaagaaag agctggatta ttattcttta ccgattatcg
    31321 atacaggaat attgaagtat gatgggaaga aggaacgatt tatctttaaa tctgttcggg
    31381 aagatttgcc tctttcagca gggatggatc tagaggtctt ggtagaagac tattttacag
    31441 aagaaaagca ctgggttcga acaaaattgg attatttacc gcaggcagcc ggagggacac
    31501 aggcaagcgg ttggtacatc actgaagaca aagagttaga actagaaggt gtaatggcgc
    31561 gtattcgaaa aaaaaattaa aaaagtaaca ttagatattt attgaaggat aaaggagcgc
    31621 aggaagtagc atgagagaaa aaagagcatt ttatatcaat ggttatgcag ggattgtcgg
    31681 attggtcgta ttagctttag ttggattgtt tttcttctat ttaggtatgt ggcaagcgaa
    31741 agtttgggca ctttttttaa gtatttttct ttggttgatc acgttattgt tgttaagttc
    31801 ggcaactgta gtcagtccta atcaagcaaa agtaattttg ttttttggac aatatttagg
    31861 tacgattcga gaaaatggct ttttccttac gatacctttg gcacaaaaaa tgactgtttc
    31921 gttgaaagtt cggaatttca atagttctgt tttaaaagtc aatgatttgg atggaaaccc
    31981 catcgaaatc tcagcagtag tagtctttaa agtcgttgat acagcaaagg cattattcga
    32041 tgtagcgtat tatcaagatt ttgtagaaat ccaaagtgag acagccattc ggcatatcgc
    32101 tagccaatac ccgtatgata cattcaatga gaatgatttg acgcttcgag gaaacacgac
    32161 ggcagtgtct gacgagttgc aaaaagagct gcaagagcgt ttagcagtag caggtgtcga
    32221 agtaatcgaa acacgcttga accatttagc ctatgcaaca gaaatcgcta gtgccatgct
    32281 ccaacgtcaa caagcaaaag ccattttgtc tgctcgacaa accattgttg aaggagctgt
    32341 cacaatcact caaatggcct tagagcaaat cgaagatgga caagaaatca atttcaccga
    32401 tgatcggaaa gtccaattga tcaataattt acttgtctca atcattacag acaaaggaac
    32461 gcaacctgtt atcaatacag gcgacatgaa agaccgtaag tgacatactt tttgtaagat
    32521 aggaagaagg agtttaggac ggattattgt cttgaactct ttttttagtt gttttaaaga
    32581 aaatgacaaa attgaaagaa gaatgatagt tgattcgaag gcagaattgt tcgctttttt
    32641 ctataatcag tatataaagt aagagagaag gggaaaaagt gaaaaaactt ctgatcggtc
    32701 tacttgtttt catcgttgtg gtacttgcag gggtgaaaat cgcagattac gtagttatgg
    32761 gtggagaaga atactatgta caaattacga cagatggtac aaaaacagtt tcaaaatcag
    32821 actctggaca agaaacagtc aactatagat atatcctgcc tggctatacg aaagagggta
    32881 aggaaaaaga actggacttc aagggacaat tgactcgacc gcttcgtaaa ggagcatatt
    32941 taaaaatcac ctggaacaaa aataaaggtg tcacttcgta tgaagaagtc gaaaaaaaag
    33001 acgtgccgaa aacagcactg gaaaaactaa ttaaagggta gggaaagaaa tgaacaaaat
    33061 agtcgaagta cagaatgtaa caaaagtata cggaaaaaac aatgagaaaa agacgcaagc
    33121 attaagcggc atcagcttcg atgtagaaaa aggcgaattt attgggatca tgggcgcttc
    33181 tggttctggt aagtcgacac ttttgaatat tttatccaca ttagataaac caacagatgg
    33241 gcatatccga atcaatcaaa aagatgtgac cacattaaaa ggaaatcaac tagcagattt
    33301 tcgtgcgaat gagattggct ttattttcca agatttcaat ttattagaaa gcttgacagc
    33361 acaagaaaat atcgctgttc ctctttcttt acaaggcgtc agaccaaaag aaatcaagca
    33421 acgtgtccaa aaaattgcag aacgtttatc catctctcat attttggaaa gctatccttc
    33481 tgagatttcc ggtggacaaa aacaacgggt cgccgccgca cgtgcattga tcacacaacc
    33541 aacgattcta ttaggggatg agcctacagg tgcgctggat tccaaaagtg cccgggatct
    33601 tttagatacg atggacgaat tgaatacaaa agatcatgta tcgattcttc ttgtgacgca
    33661 tgatccgttt tctgcaagtt attgccaacg tattttattc atcaaggatg gcggtatcca
    33721 tcaagaagtg aaacgtggag atcaaagccg tgattccttc tatcgagaaa tactatctat
    33781 tcttggaaac ttggaacagt agggggagat tgactatgct ttggaaatta tcacttactg
    33841 ggatcaagag ccgtttaaga gactacttgg tgttattctc aggattagtt atggcatctg
    33901 ctatttttta catgtttgaa tcaatggcaa gcaacgaagc ttttttgaaa agtaatacga
    33961 tcgttgcggc tacagttatc attttccgat tcggttctgt tttactaggg atcatcacat
    34021 tcgtttatat tttatatgca aattcctttt tgatgacgat gcggcaaaaa gactatgcaa
    34081 tgtttatgat gctgggagca aaaggcagaa aaatcgcaca gatgattttt tctgaaacat
    34141 tcttagtagg gatcgcagct actttagtgg gttcagctgt cggtgtagga ttgacaagta
    34201 tcgtccaccg attattagtg gatcagttag atctcaatat ttcgcatttt acacctttta
    34261 gtattcacgg gttgttgatc actgttttgt tttttgctgt tttgttttta ttagctgctg
    34321 ttgtcaatgc tttttcgatt gtcaaaaaac cgattttagc attgattcgt gcggatcaga
    34381 caccaacaag aatcaagcag cataaattct tgtttttcct tgaagtagta ctgggaattg
    34441 cttttctagg ctgcggttac tatatgatga aacacgtggg gacctatcaa gtctttggta
    34501 taggggtagc cttagttacg atcgtattag gaacttattt cattttccat tctgtgatca
    34561 ttttcttttt atcattacta aagaaatcag aacagatttc attgaaaaaa ttgaacaatt
    34621 tcacattgtc acagttaaac tttcgtattc gtgaatatac acaaatgctc tcaatggtcg
    34681 ctatgctgtt tgccttggca ctgggcgctt tgacggtagg attaggtttt aaaaatgaaa
    34741 ttgcagactt gacagaaaac aattctggat atgatctagt gatgaataat cctcgggcag
    34801 aagatcagca aaaaatcaaa gaactttcac caacactttc tgcttcttat actcaaaaag
    34861 aagatgcggg aacgatttac tataatgcaa gtgaatttgc ggctcaacca ttgatcatga
    34921 taaaacatga agatggaaca ccgccgaaga tcacgaaaga aaaagttcct gctaaaaaaa
    34981 tgaatgaact gactgtacaa gaggaacttc gaagctatga attgccggaa cagcaagaaa
    35041 aagaaatcaa ggtagtcagt caatcagaat ttgatcaact gaacttgcca caatccactt
    35101 tacagctagt tcaggtaaaa gacttccaag caaacctgaa accaatcgaa acacttgttg
    35161 ctcaaaataa aatgaacaat ccatctttgc aaaaagtaga gtacagcaac cagaaatatg
    35221 aaatgtatga agtgtataat ggtctattct ctggttttga atttatggga ttcttcctag
    35281 gtttagcttt cttgaccatg cttgctagct gtttgatgtt caagatcttg tcaggaagca
    35341 agagtgacat tgctcgttat gaaatgcttg aaaagatagg aacacgacgc gggttgctta
    35401 aacaatcgat ccgtagagaa gtaggcgtgt tattcttagc accaggaatc ttaggtgtag
    35461 tccatgtctt attcggatta caaatgtttg gtctattcat gcaaaatcca tacaaagata
    35521 tttgggttcc gtttacgata ttcttcgtat tgtatttcat ctactatgta ctgacgacat
    35581 ggttatatac tggaatcgta ttgaaaaaga gattgtgaaa aagctgtcct gcatcaagta
    35641 ataagaacag ccaagcaaaa atagctcctc atatttttcg tttggttggg cttattaccg
    35701 cagatgcaag cttttgaaca ccgtttgttc ggaaaaagag gttgtgacaa tatttttgtc
    35761 acaacctttt tttcacggtc ttttattaga atatagtaaa ttttttctcg ttactcacgg
    35821 aataagctgt taagaaaacg gtttccatgt atactggaat cagtgcaaat gaaagaaaag
    35881 agggtgcaag gaacatggaa atcaatgaac aaaattttgg cgaaaactat cacttgatta
    35941 cgatcacgaa tcaaaatggg acatctttgt ctgtcagtga tcttggggca cgggttgttc
    36001 attttcaaac aataatcgat aatgaaaaac gagagttgat cttaggtttt gatgaagcaa
    36061 aagaatatct agaaaaagac ccttatatcg gtgcttcaat cggacgtatg gctggacgga
    36121 ttacgaatgg cacattcaaa ttagatgatg aaacgttcga agtaaaaaca gacccggaca
    36181 ctggtcattg tttacatggt ggtgcaccta gctttgaagc caagcagtgg cattatacag
    36241 tcatcaatgg tgaaaacgaa gcatcggtca ttttctatca gactagccca gatatggaaa
    36301 acggatttcc tggaacttta gatgtagaag tacggtatac gctgacgaac gatgatacat
    36361 ggcgagtgac ggtccaaggg atcagtgacc gcaaaacagt attcaatcct acaaatcatg
    36421 tctatttcaa tttaaccggt gatccagcac agtcaatcga cgaacatata ctttggttga
    36481 atagccgatt atttgctacg ctcaataaag atacaacgcc tacaggagaa gtagtttcag
    36541 taagtggcac agcatttgac ttccaacgtc ctaaaaaatt aggtgaggtc tttgccagtg
    36601 attttcgcca atcaatgata gtgggcggca tcgatcatcc atttttgttg aaaaaacaat
    36661 ctggtttact aaatgctgcg gaattgacaa gcccagatga aaaagtatcg atccaagtaa
    36721 aaacagatgc ttcaggaatc gtgattttta cagcaaattt tggagaagat ggaccggaaa
    36781 tgcgcgggaa taaattagta aatcatggcg gtatcacatt tgagactcaa gaactgccag
    36841 gagcggagcg acttgaaagt tttggagatg tcactctgga acctaaccaa gtacgcgagt
    36901 ttgtaacgga atacaaaata aatataaaga aatagaataa attaaaa
//
LOCUS       DANKMN010000020        36575 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000020 DANKMN010000000
VERSION     DANKMN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PW224_000991"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PW224_000991"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL2907698.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PW224_000992"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PW224_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL2907699.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PW224_000993"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PW224_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL2907700.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PW224_000994"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PW224_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL2907701.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PW224_000995"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PW224_000995"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL2907702.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PW224_000996"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PW224_000996"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL2907703.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="PW224_000997"
     CDS             complement(8053..8523)
                     /locus_tag="PW224_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL2907704.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="PW224_000998"
     CDS             complement(8569..8994)
                     /locus_tag="PW224_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL2907705.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="PW224_000999"
     CDS             complement(9061..9330)
                     /locus_tag="PW224_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL2907706.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="PW224_001000"
     CDS             complement(9341..10936)
                     /locus_tag="PW224_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2907707.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALCRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PW224_001001"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PW224_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL2907708.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="PW224_001002"
     CDS             complement(14489..15049)
                     /locus_tag="PW224_001002"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL2907709.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="PW224_001003"
     CDS             15402..16376
                     /locus_tag="PW224_001003"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2907710.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="PW224_001004"
     CDS             16546..17946
                     /locus_tag="PW224_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL2907711.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="PW224_001005"
     CDS             complement(18024..18746)
                     /locus_tag="PW224_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2907712.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="PW224_001006"
     CDS             complement(18825..19331)
                     /locus_tag="PW224_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2907713.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="PW224_001007"
     CDS             19454..21253
                     /locus_tag="PW224_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL2907714.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="PW224_001008"
     CDS             complement(21321..22895)
                     /locus_tag="PW224_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2907715.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="PW224_001009"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="PW224_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL2907716.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="PW224_001010"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="PW224_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL2907717.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="PW224_001011"
     CDS             complement(24672..25310)
                     /locus_tag="PW224_001011"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL2907718.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="PW224_001012"
     CDS             complement(25311..25961)
                     /locus_tag="PW224_001012"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL2907719.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="PW224_001013"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="PW224_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL2907720.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29113)
                     /gene="pknB"
                     /locus_tag="PW224_001014"
     CDS             complement(27038..29113)
                     /gene="pknB"
                     /locus_tag="PW224_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL2907721.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYNYSDTVPSGSIISQDPASGEVVAEET
                     YINFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVP
                     GAGTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSS
                     SASTSESSK"
     gene            complement(29110..29850)
                     /locus_tag="PW224_001015"
     CDS             complement(29110..29850)
                     /locus_tag="PW224_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL2907722.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29863..31221)
                     /gene="rsmB"
                     /locus_tag="PW224_001016"
     CDS             complement(29863..31221)
                     /gene="rsmB"
                     /locus_tag="PW224_001016"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL2907723.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31221..32159)
                     /locus_tag="PW224_001017"
     CDS             complement(31221..32159)
                     /locus_tag="PW224_001017"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL2907724.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32152..32643)
                     /gene="def"
                     /locus_tag="PW224_001018"
     CDS             complement(32152..32643)
                     /gene="def"
                     /locus_tag="PW224_001018"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2907725.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32658..35066)
                     /gene="priA"
                     /locus_tag="PW224_001019"
     CDS             complement(32658..35066)
                     /gene="priA"
                     /locus_tag="PW224_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL2907726.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35319..35624)
                     /locus_tag="PW224_001020"
     CDS             complement(35319..35624)
                     /locus_tag="PW224_001020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2907727.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35629..36243)
                     /gene="gmk"
                     /locus_tag="PW224_001021"
     CDS             complement(35629..36243)
                     /gene="gmk"
                     /locus_tag="PW224_001021"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL2907728.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9888 a         7990 c         6706 g        11991 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggcaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gttatagtca taagtagcac taatattatt gatattgaat ccttgtgtaa
    27541 caagccaatt acgaacagtt tcgtagtttt ctcctacatg actactcaaa gtcactgttt
    27601 ctggtccttt gctgactgtg aaagtaatcg tgtctgtttt aggatcgact tctgtatctt
    27661 ctgatggaga ttgcgaaatg atcattccgc tttctacctc tgaactgaat tcctcttctt
    27721 tttgaatcaa gtcagcagaa aaccctaatt ctttcaaact tgcttcggca tcttcataac
    27781 ttttaccact gtaatcatct aatttgattt ttttcgtgcc gctactaata tacagagtga
    27841 cttcgcgttt ttgcttgact gtcgttcccg ctgccgggtc tgtcttcact acattacctt
    27901 cttcgatttt gtcatctgca atttcttctg tttttgttgc tacttctaat ttcgcttttg
    27961 cgagtgtttc tctagcagaa gcttcagaaa gatcagaaac atcaggaaca acaacttctc
    28021 ctcgcccgcc agaagcaaag aatacagctg ttccgattcc taaaacagca agaatagcta
    28081 ataggatcag ccaccatttt ttctgtcctt tctttttagg atcaggcaga acttcttcct
    28141 cctctggcaa ggtttcgttt tctggcttgt cttttggcag aggcatagat ttgaatgaat
    28201 caggcattgg tgcatcttcc gtaaggggtg tgataacttt tgtttcatta tccatcacat
    28261 gaggctgcca tttttcttcg tttgcacgag ctggtgataa tactgtagaa agatcatcag
    28321 acatttcctc ggctgactta taacggtctg caggttcttt agccgttgcc cgtaaaatca
    28381 cgttttccag tgcttgtggc acatttggat caagtgcttt tatagatgga agatcatctt
    28441 gaaaatgttt taaagcaatt gttacagcag attcaccatc aaacggtacg ctacccgtca
    28501 acatctcata aaggatgatc cctaaagcat aaatatctga ctgcttcgtt gccatacttc
    28561 ctctggcctg ctcaggggac agataatgaa cagatcccag catcgtattc gtctgcgtga
    28621 tcgatgtttc agataaagcg atagcaatcc cgaaatccgt gatttttaca acaccttcat
    28681 tatcgatcag tacgttttgg ggtttcaaat ctcggtggat gatttgatgg ttatgagcta
    28741 aagaaatagc agaaaggatc tgctgcataa tatttactgc tgtttcataa ggaacaggat
    28801 aatgtgtttg gatatatcgt ttcaagtcca ttcctttgac gtattccatc accagatact
    28861 gcatgttgtc ttcttcacca acatcgtaaa cactaacaat gtttggatga acgagttctg
    28921 ttgctgctaa agcttctctc tgaaatcgtc gaatcgcagt ctggtcattt tgaaagtcaa
    28981 atcgtaagac tttaactgct acatcacgat ccaagattaa atcgtgcgcc aaaaatacat
    29041 ttgccatacc accgctaccg atatttccag tgatttgata acggccattt aattttttgc
    29101 ccaactcgat catacactgt cctccttata ttcgataaga agaacggtga tattatccgc
    29161 tccgcctgct tcattggcac gatcaatcag ctcagttact ttatgagaca atgggccttc
    29221 ttgattgatg atcgttccta tttcttcttc agacagcatg ttcgtcaagc catctgaaca
    29281 taacagcagt cgatctttta gttgccaaag atgagtagaa acatctactt cgactgttcc
    29341 aggcatgccg acagaacgtg ttaaaatatt tttccttgga tggttcactg ccatttcttc
    29401 gctgatttct cctgatttga ctagttcatt gactaaagaa tgatcctccg tcaattgcat
    29461 gattttatct tcacgaatca aataagctct gctatcccca acattggcaa tcgtgaacca
    29521 gtcatctgac agagcggccg ctacgattgt tgttcccata ccgttatatt ccggctgttc
    29581 ttgtccacgc tgataaatca gcatattttc ttcttgaatc gattgaatga accattgggc
    29641 gattttttca tcgtcaacag cttcttgttt ttcccaagct tcgcctagat tcgttaccgc
    29701 catctgactg gcaatgtctc cggcacgatg accacccatc ccgtctgcta atacagcaag
    29761 cttgagacct gaacggctgg taaatacact ggcatagtct tgatttgtgt tacgtcgtct
    29821 tcctacatct gattgatatt caatctgcat tttttcacct cattactttt tgcgcaaaca
    29881 acaaatgaaa aatccatctg tgtaaaactg atgcggatac aaagtcaaca ttttctcttc
    29941 gattgatgat tgtagcatag gattggtcaa aacatcaatc ctctcaaaat cagggtgtct
    30001 ttccaagaaa gcagaaacaa cgtcttggtt ttcttctttt aagatcgtac aagtgctata
    30061 agtcagaata ccggaagact ttaaagttgg tgcgcagctt tctaaaattt ccaattggat
    30121 ttttggtaaa ttcgctaatt catccggctg ttttttataa cgaatatctg gttttcgccg
    30181 aagcagccca agacctgaac aaggcgcgtc tactaaaatc cgatcaaact gctctgcttc
    30241 gaactgttta ctgacttctc tagcatccat cttttgagcg tagacagcag aagagacacc
    30301 tagacgtgct gcattatctt caatgagttt gactttgtgt tcgtgtacat ctaaagcgac
    30361 cacttctcca ccgcatgacg gatctaaaaa agaagcaata tgcgtcgtct tacctccggg
    30421 tgctgcacag gcatctaaga catgatgttc aggctcgatt tgcatagacg gcgctacaag
    30481 catcgaactt tcatcttgga tcgtcatgac gccatcgtgg aacaatgagc tagaagctaa
    30541 atgccctttt ttcgctacta taccgtatgg actgacttgg cttttttcag ctgtgatctc
    30601 ttcttcttgc aaaacagtga tggcctgatc tctggaaagt cggcgtgtat ctactcgggc
    30661 actagcatgg ctaggctgat atagcgataa accgagcttt tcagtttctt cgtatccgat
    30721 ttgatccacc agtcgtttcg tcatccatag aggcaggctg attttgacag aaagtctttc
    30781 gataggatca gcaatgcctt gcagatctgg tgctccttga cgctgataat tcctcaagac
    30841 accatttaca aatttccctg tcccaggatt tccttttact tttgcgattt cgaccgcctc
    30901 gtttaaaata gcatgtgcag ggactttatc taaaaattct aattggtata cagaaagata
    30961 aagaagcgtc ttgacccagt catccacttt ttttgcttta gcgatcaatg gtgcaacgta
    31021 aaattccaat agtcgctgat ggctaatcgt accatagatc aattctgtaa ataaacccac
    31081 atctttttga tttagctcgc tttgtttgat catctcattc aacaaaagat tcgagtaggc
    31141 gccacccttg ttcactcgct ctaatgtcag taatgccata tatcgtactg attttttcat
    31201 ttttttaggg atttttttca ttatttcacc tgctgaccaa tctgaacttg ctgaccgctt
    31261 ccgttaagga aatcatgtac ggcctgtttt cctttaccag ctggctgtaa ttcattgatg
    31321 gctaagacgg tctgtttacc gcaagcaatc catagatttt ttttgtcttt ccggatgatc
    31381 gtaccaggtg ctttgtctgt agtttctgcc aatacttcaa ccgataataa tttccagcgt
    31441 atgccctcat aggtcgtgaa tgcaattggc caaggacgca ttccgcgtac ttggttgtca
    31501 atttcttctg cagttttatg ccaatcgatt gcttcttgtt cccgtgtgat gtttggcgaa
    31561 aaagttgctt ctgactcgtc ttgaggtact ggtttttgtc catccaaaat attgggcaat
    31621 gtttctagca atagctttct tcctaaaagg ctaagctttt caaacatcgt ccctacatca
    31681 tcttgtttag tgattggtat gctttcttgc gcatagattc cgcctgcatc catttttttg
    31741 atcatttcca tgatcgttac accagtttct ttctccccat tcatgatggc ataatgaaca
    31801 ggcgcacctc cccgatattt aggaagtaaa gaagcatgga cattgattgc ccctaatttt
    31861 ggtacttgca ataacttttc aggcaagaat tggccaaatg ccgcagtgac gatcacatca
    31921 ggcaccaatt caatgatttc ttccatttct ggcgaaccgg agattttctc tggctgcagc
    31981 acacggatac cgtgcttcag agccgcttct tttaccggtg tcggcgtaat tactcgtttc
    32041 cgcccaactg gacggtcggg ctgtgttacc actgcctgga tctcgtaacc tgattctatc
    32101 agcccttcaa gaatcggcac agaaaaagca ggtgtgccca taaatactag cttagtcatc
    32161 aagatgttcc tccatatatt cttcaagttc ttcttccggg atctgctgta tcattttctc
    32221 aatgaacaag ataccgtcta agtgatcgat ttcatgctgg aaggcacggg ctaaatagcc
    32281 aaaagccgtc acttctatct cttctccgtc tcgatcataa taacgcacgg tcacttcatc
    32341 tgctcgcttg accgttccat agacatgcgg aatactgaga caaccttcta cgtcaaggct
    32401 ttcacctttt gcttcgatga tttctgggtt aatcagttca aatttctcgc cttcatctac
    32461 ttcgatcaca gcaattctct tgttttgtcc tacttgagga gcagcgatcc ctattccgtc
    32521 attcgcaatc attgtctcat ataaattgtc taataaagcg attgtctcgt ccgtgattac
    32581 atcgattggc tgagcagttc tttttaattt atcattaggg tggatcaata taggatagcg
    32641 cataatttgg ccctttctta aataaagttt aatggttcgt tgtcaattgc cacaaaaaga
    32701 ccttgtcgct gttctttctg gcttccattc aaaatttcat ccaaaacatg aggaagattc
    32761 ggttcatgtt tatatttgat gattagttga taataatatc ggtttttcac tctcaaaatc
    32821 atgctgggcg ttggccctaa tagcaagctt tgagggctta acacttcttt tagctgattg
    32881 gcaatctgaa agattttctt tgccgctact tgttcttcag gatggctggc ggttattttc
    32941 acagtaaagt aataaggagg atagcctcca cgatgccgta catgcatttc ctgctgataa
    33001 aactgctcat aattttgttt tttagctaat tctatggcgt aatgttgagg attgaatgtt
    33061 tggatcacga cttcgcctgc tttttccgct cgccctgctc gtcctgaaac ttgggtaagc
    33121 aattggaatg tatgctcact agaacgaaaa tccggtaaat tcagggaggt atcagcattc
    33181 aacaccccaa ctaatgtcac ttctggaaag tccaaacctt tagcaatcat ttgtgtccct
    33241 aataatatat ctgcttcttt tgcaccgaat ttttgtaaaa tctgttcgtg tgcccctttt
    33301 cttcgagtgg tatctacatc catacgcaaa atcctagctt gcggataaag ttctcggagt
    33361 tcctcttcta ctttttgagt acctgttcca tagtagcgga ttttattgcc gccacaatta
    33421 ggacagcgat tgggaatagg ttcttcatgt ccgcaataat ggcagcgcat tgaacgtgta
    33481 tccatatgca gtgtaagaga gatatcgcaa ttaggacatg gaagaacaaa tccacagtcc
    33541 ctacacatca caaaagacga atatcctcga cgattgagca gtaggacagt ttgttctttt
    33601 ttgtctaatc gattttgtat tttttcctgc aaattagcag aaaaagtaga ggtccgatga
    33661 ttctgaaact cttcacgcat atctacgatc tcaatagctg gaagctgtgc agctgctttg
    33721 gctcggtggt ttaactgcaa taactgataa acgccttttt gagctcgtgc tcttgattct
    33781 aaagagggcg tcgcgcttcc tagaactatt gggcaatgat gatatttact gcgccaaatt
    33841 gctagatctc tcgcatgata acgcggtgtc tcatcttgtt tgtaacttga ttcatgttct
    33901 tcatcgataa tgatcagccc gatatttttc agcggggcaa aaatagcaga tcgtgcgcct
    33961 actactactt gcgcttcttc ccgctcaatc ttgcgccatt catcatattt ttcaccttgg
    34021 gaaagtccgc tatgtaagac cgcaactgat tctccgaagc gtcctttgaa tcgctcgacc
    34081 atttgcggag tcaatgcgat ttcaggcact aacataatag ccgttttatt ttctgacaaa
    34141 acatcagcga ttgcctgaag atatacttct gttttcccgc tgccagtgat tccctccagc
    34201 aaaaagacct gatctttttg ctgctgacct gctgtgatga tttgttcgac agcatttttt
    34261 tgttctgcat tcagttccaa tgccgtcgtt tggtcaaata ctcggtcttt ataagggtcg
    34321 cgataacgtt ctgattctat aaactccagc cagccctttt cttttccttg attcaagaca
    34381 gcagtactga ttcctttatt tttgaaataa gcaattggtt cttcttggct cagttcagat
    34441 aaacacaaaa gaagttcttt ttgtttcttg gcattttgcc gtaattttgc ccattcttct
    34501 tcgatttgct tttcagttaa caaagaacgg accaaacgaa tcgtttttac tttatttcgt
    34561 gtatggactt catatttgat atctactaat tgctcttggc gccagcgaat caattgagaa
    34621 aggctgcctt cttcttctgc ttccttccag gaaatcactt ctcgctgatc aaacaatacc
    34681 tgaacttctg gtgtgtctgc caaaggatag atcaatttat catattctgc tttcatcaca
    34741 ctcggaagca tcgtttgtag acaagtgatt ttgaatgcgt aagtaatttt tttcatataa
    34801 tcagctaatg aaagtaattc ttcattgacc accggagcaa ggtctagcaa acgaataact
    34861 gccttcaaag atggattagc tgattcttct gatttttgga tagccataac aaatccttgg
    34921 acatgacgat tgccattacc aaaagggact tctactcgca tcccgacttg caaagctgtt
    34981 tccatttcag agggaaccaa ataagtgaat ggctgatcgg tctgcatagt aggtacgtcc
    35041 acaatgactt cagctgttaa tgtcatggtt cgtttcctcc tttttttgtc ttttcttatc
    35101 aagcgacaaa aagttccttg cctattttac tagagatcga tggatttgtc ttatcttctg
    35161 ccaaatccta acaaaaaatt aacagcagat aagaaaaaaa agaactgtgt caaaaagttc
    35221 agacacagcc ttttctacca gacaataagt ggcgaatgtt tgaaaaggaa acagctgggt
    35281 ttgcttttct ttttagatgt tcgctctatt tttctttatt acattttttg ctcttcacgg
    35341 atacgtgatt ccaattcgtg ttgttcacgc tctttttgtg cttgacgttc ctcttcttcc
    35401 atcttcaaac gttcacgttt taattcagga tgtggatcat tgactacatt tcctgcatcg
    35461 atttcttcta aagcttgacc gacgcttttt actgaatcaa aagaatctag agtaggctgt
    35521 gcacttgcat ccaattcatg cgcgcgttta cttgctaaaa tcactagaga atattttgaa
    35581 tttacacgat ctaatagtga gtcaattgaa ggttttaaca tcataagact acatctcctt
    35641 taacatttta atatatttac cgattacgcg atctactcga aaatgttcac tggcaataat
    35701 gtttttgatt cgttcaactg ctagcggcac ttcatcattt acaacagcat agtcgtaaag
    35761 agccatcatt tcgatttctt cttttgcaac tttcattcgt tcttcgatta cttcatctgc
    35821 atctgttcct cggcctacga tccgtgattt taattccgct aaatctggcg gagtcaaaaa
    35881 gatgaataca ccatcgggca ctttttcttt gacttgtttt gctccttgga cttcaatctc
    35941 caagaaaaca tcttttcctt catccaatgt cttgttgata tatgacaaag gagttccata
    36001 ataattcccg acatattctg cgtattccag catctctcca gcttcgatca ttgattcaaa
    36061 ctcttctttg cttcggaaga aataatcaac gccttcgacc tccccttcac gcattttacg
    36121 ggtagtcatt gagatcgaat actgaaaatc attatcctca ctctcaaaaa ttgcttttcg
    36181 tactgtgcct tttcctacac cggatggacc agacaacacg attaataatc cacgctctga
    36241 catgacgacg tcctttcaac tagtcttttt gtacttatcg tttattttgc actttttttc
    36301 tgcaactttc aaggttatct ttaattatag ctagaaattg gtaaaaaagc taccctgtac
    36361 caagaggtcg taaaaaagct gtcctgcatc aagtaataag aacagccaaa cataaacagc
    36421 ttctcatgtt tttcgtttgg ttggggttat tatcgcagat gcaagctttt gaacgccgtg
    36481 tataagaggt cgtgaagaag ccgattagtt tcgagtatga agaaaaattt tgtgaaaata
    36541 gtttctcaca tttttgcata aatttggctt aatac
//
LOCUS       DANKMN010000021        35539 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000021 DANKMN010000000
VERSION     DANKMN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            219..1985
                     /locus_tag="PW224_001022"
     CDS             219..1985
                     /locus_tag="PW224_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL2907729.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2087..3346
                     /locus_tag="PW224_001023"
     CDS             2087..3346
                     /locus_tag="PW224_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2907730.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFQKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3493..4794
                     /locus_tag="PW224_001024"
     CDS             3493..4794
                     /locus_tag="PW224_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2907731.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4797..5648
                     /locus_tag="PW224_001025"
     CDS             4797..5648
                     /locus_tag="PW224_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2907732.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5661..6476
                     /locus_tag="PW224_001026"
     CDS             5661..6476
                     /locus_tag="PW224_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL2907733.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6627..7793
                     /locus_tag="PW224_001027"
     CDS             6627..7793
                     /locus_tag="PW224_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2907734.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8023..8670
                     /locus_tag="PW224_001028"
     CDS             8023..8670
                     /locus_tag="PW224_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2907735.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8754..9698
                     /locus_tag="PW224_001029"
     CDS             8754..9698
                     /locus_tag="PW224_001029"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL2907736.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9833..11284
                     /locus_tag="PW224_001030"
     CDS             9833..11284
                     /locus_tag="PW224_001030"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL2907737.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11305..12141
                     /locus_tag="PW224_001031"
     CDS             11305..12141
                     /locus_tag="PW224_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2907738.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12141..12800
                     /locus_tag="PW224_001032"
     CDS             12141..12800
                     /locus_tag="PW224_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2907739.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12926..13615
                     /locus_tag="PW224_001033"
     CDS             12926..13615
                     /locus_tag="PW224_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907740.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13621..14577
                     /locus_tag="PW224_001034"
     CDS             13621..14577
                     /locus_tag="PW224_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL2907741.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14621..15133
                     /locus_tag="PW224_001035"
     CDS             14621..15133
                     /locus_tag="PW224_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2907742.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15389..15937)
                     /locus_tag="PW224_001036"
     CDS             complement(15389..15937)
                     /locus_tag="PW224_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2907743.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16026..17207)
                     /locus_tag="PW224_001037"
     CDS             complement(16026..17207)
                     /locus_tag="PW224_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL2907744.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17443..18951)
                     /locus_tag="PW224_001038"
     CDS             complement(17443..18951)
                     /locus_tag="PW224_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL2907745.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19110..19301
                     /locus_tag="PW224_001039"
     CDS             19110..19301
                     /locus_tag="PW224_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907746.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19504..20421
                     /locus_tag="PW224_001040"
     CDS             19504..20421
                     /locus_tag="PW224_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL2907747.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20414..21487
                     /locus_tag="PW224_001041"
     CDS             20414..21487
                     /locus_tag="PW224_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907748.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21635..22153
                     /locus_tag="PW224_001042"
     CDS             21635..22153
                     /locus_tag="PW224_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907749.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22134..22526
                     /locus_tag="PW224_001043"
     CDS             22134..22526
                     /locus_tag="PW224_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL2907750.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22602..23537
                     /locus_tag="PW224_001044"
     CDS             22602..23537
                     /locus_tag="PW224_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL2907751.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23670..24449
                     /locus_tag="PW224_001045"
     CDS             23670..24449
                     /locus_tag="PW224_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2907752.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24442..24759
                     /locus_tag="PW224_001046"
     CDS             24442..24759
                     /locus_tag="PW224_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2907753.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25003..26613
                     /locus_tag="PW224_001047"
     CDS             25003..26613
                     /locus_tag="PW224_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2907754.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26770..27618
                     /locus_tag="PW224_001048"
     CDS             26770..27618
                     /locus_tag="PW224_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2907755.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27849..28328
                     /locus_tag="PW224_001049"
     CDS             27849..28328
                     /locus_tag="PW224_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907756.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28361..30589
                     /locus_tag="PW224_001050"
     CDS             28361..30589
                     /locus_tag="PW224_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2907757.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30718..32361
                     /locus_tag="PW224_001051"
     CDS             30718..32361
                     /locus_tag="PW224_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2907758.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32434..32979)
                     /locus_tag="PW224_001052"
     CDS             complement(32434..32979)
                     /locus_tag="PW224_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL2907759.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33002..33487)
                     /locus_tag="PW224_001053"
     CDS             complement(33002..33487)
                     /locus_tag="PW224_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2907760.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33477..34289)
                     /locus_tag="PW224_001054"
     CDS             complement(33477..34289)
                     /locus_tag="PW224_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL2907761.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34428..>35539)
                     /locus_tag="PW224_001055"
     CDS             complement(34428..>35539)
                     /locus_tag="PW224_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2907762.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLQSLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11626 a         6395 c         7109 g        10409 t
ORIGIN      
        1 attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag gaagtagact
       61 aaaactttta aaagttttaa gtctatctat ttggcaagtt taaaaaataa aaattcggta
      121 tcaaaaaact gttaccgata acatttttga aagcgttgtc ttttgcttct ctatcactta
      181 tagtgatgac gtcatattaa aacacaagga ggaaatcaat ggatacagct gcaatttatc
      241 atcgaccgga aagtgaatat gcatttctat atacgaatac aagatttcgt attcggcttc
      301 gtactaggaa aaatgatatc aaaaaagtat atgtgctatg tggagatcca tataccataa
      361 caactgaaaa atggtatcaa aaacaacgac caatgaaaaa atgtctaagt accaatgttc
      421 atgattattg ggaaattgaa gtgacgagcg aaacaagtcg cttacagtat gcgttccatg
      481 ttgttgggga agataacacg gactgttttt acggtgatca aggaattttt ccttatcgag
      541 aaaatgtgat tactgaacca aatttttatt ttcgaatacc ttattttcat caaatcgatc
      601 gattcactat tccagaatgg gtaaaagaaa cagtatggta tcaaattttt cccgaaagat
      661 ttgcaaacgg ggacaaagca aatgaccccg aacacacttt accttgggga agtaaagatc
      721 cagaccgaga agactttttc gggggagatt tgcaaggtgt gatggatcat ttggattact
      781 tgacggacct tggcatcaat ggcatctatt tttgtcccat atttaaagcc acatccaatc
      841 ataaatacga tacgattgat tattatgagg tggatcccgc ttttggcgat aaaaaacttt
      901 taaaaaactt gattgatgaa gcacataaaa gagggatacg tatcatgttc gatgctgtat
      961 tcaatcatat gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat
     1021 cgatctataa agactggttc cacatccgtt ttttcccagt agattcttat cagatgactg
     1081 acttaccaga aactgctgaa aatatcccat atgatacttt tgcttttact ccattcatgc
     1141 cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt gctggatatc gcaacttatt
     1201 ggataaaaga attcgatatt gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc
     1261 atttttggaa aaaattcaga gaagcagtta cagaaatcaa accagacatc tatatccttg
     1321 gagagatttg gcattcctct caagcttggc tgcaagggga tgaattccat gcagtgatga
     1381 attatgcatt tacagattcg attaaagatt attttgcaaa aaagaaaatc acagcttcac
     1441 aaatggtcag tggaatgaat catcagcaaa tgctttacag agatcaggtg aacgaaggaa
     1501 cattcaactt attagattct catgatactg ccaggatctt gaccctttgc caaggtaaca
     1561 aagaattgat gaagtctgtg atggccttta tgtttcttca aaaagggtct ccttgtatct
     1621 attacgggac agaaataggg atgactggtg aagatgaccc ggattgccgc aaatgtatga
     1681 tatgggagaa ggaggaacaa gatctagagc tattcggttt tataaaagaa cttgtttctc
     1741 taaggaaaca gttgtctaag attatttctg aaggttctac tcagtggcta atagtaaatg
     1801 atcgtgaaga taagctctat ttcacacgga aactagaggg acagatcatc tatgtttact
     1861 ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa
     1921 attgtcagtt gttagaagac gggaaagcag agatacgaca atacggattt ttgatcatgt
     1981 gttgaaaact gttaccggta acaacaagaa agcgtttgca aatgaagatt tgtgcccatt
     2041 ataataaggg tgtaacagca cgttgaatac aaaggaggaa atgaagatga aaaaaagaag
     2101 tgtgaaacag ttgggtttag ggttaatctc tttaggaatc ttaggcggat tagcggcttg
     2161 tggcaatggt aactcttcag cagaagggga agccgatgaa ggaaaaacgt taacagtttc
     2221 cgttgattct ggatataaag attatataaa cgaaataaaa aacactttcc aaaaagaaaa
     2281 cgatgtgaaa atcaagctag tagaaaagga catgttcgat cagttggaat cattagcgtt
     2341 agatgggcct gctggaaagg gacctgacgt tatgatggca gcatatgacc ggatcggtgc
     2401 gttaggacag caaggacatc tggcggaagt aaaactggga aatgaagcag cttacgatga
     2461 aacagacaaa gcccaagtga cttatgatgg aaaaatctac ggagaacctg cagtaatcga
     2521 gacccttgtt ttgtactata acaaagattt agtcgataca gcaccatcaa cgtttaaaga
     2581 tttggagaat ctatccaaag acagtcgttt tgcttttgag tcagaagcag gaaaaaatac
     2641 aggattttta gctaaatgga ctgactttta ttattcctat ggtttaattg caggttacgg
     2701 tggctatgta tttggtgatg atggcacaaa tccttcagat atcggtttga ataatgcagg
     2761 tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa aatgtttggc caaagggaat
     2821 gcaagacata aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca ataaaactgc
     2881 agcaatcatt gacggtccat ggcaagcgca aacatacaaa gaaaacaata tcaattatgg
     2941 tgtagccaaa attcctactc taaataatgg acagccttat caaccgtttg gcggaggaaa
     3001 aggatgggta gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat ggcttgatta
     3061 tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa
     3121 ccaacaagct agggaaacag caaaggctaa aaacgatgaa ttaacaacag ctgtgattga
     3181 acaatatgaa acagctcaag cgatgccaaa catcccagaa atgggcgaag tttggacagg
     3241 tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag aatccgcaga
     3301 tgaagcagta aaaacaatca gtgaagccat agaacaaaaa tactgattaa aatgataacc
     3361 gtccattttt ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga gcgagatgga
     3421 aaattatctt gctcttgttg ccattatctt aaaagcagtt tgcggtttag caagtttaga
     3481 gggggcattc agatgcagca agataaaaca agcaatatca acgaaaaaaa aatcacgctt
     3541 tgttcactta ttccagggat ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt
     3601 tttttcgcca tattcgtcct attcattggt cagatgcttc tgggcggatt tcatgcattg
     3661 gtaggattgg ttactttggg aagtatacca atggaagatc attcattatt tattttgatt
     3721 gaaggcactt tgcaattcat cattacaatc attttcttga ttttttacgg tttgaatctt
     3781 ttagacgctt atcgagtagc gaagatgcgt caagagggca aaaaaatcaa tcggacgatg
     3841 aaggaagtca tacataatat aggtgatgaa ggttttcctt atctattgac acttcccgct
     3901 tatcttctca tgatttttac gatcattttc ccagtcctag ttacattgtt cactgcattt
     3961 actaattatg acttcaatca tattccacca gccagtttaa ttgattgggt aggggctaaa
     4021 aactttttta gtatcttctt tttaagctca tatcgtaata ctttttttgc agtcttttcg
     4081 tggaccgtga tttggacttt atgtgcaaca actttgcaaa tcacccttgg tattctcaca
     4141 gcggttgttg ctcaccagaa atttattaaa ttcaaacgta ttttcggtat cattttctta
     4201 ttaccatggg cggtcccagc ttttattact atcatgagtt tttcgaatat attcaacgat
     4261 agcgtaggtg ccatcaatac tcaagtgatt cctttcataa accagcttcc ttttgtagat
     4321 atcagtgcaa tctcttggaa aacggacccg aactggacga aagtagcaat tatcttagtg
     4381 cagggctggt taggatttcc ctatatctat gtgatggtta caggaatctt acaatcgatt
     4441 tcagaagact tatatgaagc agcaaaaatc gacggagcaa atgcatggca gcgcttttca
     4501 aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa catttattac acagtatacg
     4561 gggaatttca ataatttctc catgatctat ctgtttaaca atggcggtcc cggaagcgta
     4621 ggcggaggag ctggttctac tgatattctt atttcctgga tctataaact tacgactgga
     4681 acttctccgc agtattctat ggcttcagca atcacgttga ttatctccgt catcgttatc
     4741 tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca atatggggga ttaacaatga
     4801 aaaaatttca ttcgagtgct cgattccaaa aacgactttc tcactttttt acttactttt
     4861 ttcttttaat actgtcagtc attatcatct atccgctttt gatcacagca agtactgcct
     4921 tcaagtcagg aaatattgca gcttttagtt tagatctttc aagtgactgg actttaggaa
     4981 actttactcg gctattcagt gaaacacttt acgggacttg gtataaaaat acattgatca
     5041 tttctgtcct aacaatgatc gtacaagtga ctgtagtaac tttggcgggc tatacttata
     5101 gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt ctttttagtg attcagatgg
     5161 taccgactat ggcagcattg actgcctttt atgtcatggc tttcttatta gaagcgctcg
     5221 ataagtactg gttcttgaca atgatctata taggcggtgg aattccaatg aatacttggc
     5281 tgatgaaagg atactttgac actgtaccaa tagatttaga tgaatctgct aaacttgatg
     5341 gggcagggca tttcaggatt tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg
     5401 ccgttcaagc attatgggct tttatgggac cttttgggga tttcatgtta gctaagttcc
     5461 tgcttcgaac acctgaaaat cagactgttg ctgtcggctt acaaacattt atttctgatg
     5521 tgacaaacca aagagtctct ctttttgctg ctggggcaat tttgattgca ctaccgattt
     5581 gtctattatt cttcttttta caaaagaact ttgttaccgg cctgacatct ggcggaacaa
     5641 aaggatagga gcagagtatc atgcaaacat ttccgttaaa ctatttttcc gcattacgta
     5701 cgccaactca agtgtttgcg ggacgcaaac tattatcatg gcctaaattt ttcttgatat
     5761 ttgtattttt agtttccttg atggtcatgc ctgtgactct tttttatgcc aatcagatcc
     5821 aagcgattcc attagaacaa tttctatccg ttcattcgct gatcgatgaa caaggaaccc
     5881 aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa caaacgatag
     5941 ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg
     6001 gaacttttat tgattttgag aaggaacaat gggtgatcca acaaaaggat aaatctggta
     6061 tacgccgcta tacgatgaat tatagcccat ctttccaacc agattctgta cgtacgcccg
     6121 aagatttcca acgattttta gaaagagaat tttatgccag taatcgtcca acaatcattt
     6181 tatcttatag tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc attttattcg
     6241 gggcttcctt tttcttgtgg atgacaagaa aaagccaact ttcatccatt catacattca
     6301 aagagagtgc aaatttgatg ctgaatgtta tgggaatagg ttccatggca gcagcagttg
     6361 tagggtttat ccactttgac tttatcttga tgctaagtgt ccaaacaacg ataactgtct
     6421 tgctgctttt atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag
     6481 tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa
     6541 aatttctgaa aaaattaaac ttcttagttg attcttttct tttttagtat taacgtaata
     6601 gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat caattatttt
     6661 cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg aaacaatacg
     6721 gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag
     6781 agattacaag taacgcttct ttggcactag gagaagccta tatggatcat cgaatcgaga
     6841 tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac agctttttac
     6901 gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca
     6961 aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt tggctagatc
     7021 caactttgac ttattcttgt gcttatttca atacacctga agatacgttg gaacaagcac
     7081 aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc gaaacgttgc
     7141 ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag tataatgtca
     7201 aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa agaataaaag
     7261 aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg
     7321 aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg
     7381 aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct ttgatccatg
     7441 gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat attttccctg
     7501 gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac gatttgcaac
     7561 tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat tggacaaaga
     7621 attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc tatcgaatgt
     7681 gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc gatgtgattc
     7741 aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca taattgaggc
     7801 aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct ccagaagtga
     7861 acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc tttatatttt
     7921 cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt cttcgttata
     7981 cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa aactgttatt
     8041 tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac caatcaaggc
     8101 acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc ggactcctca
     8161 acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc aagtactcag
     8221 tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa atacccagat
     8281 actgcaatta ctagtttgga attagatagt gattggggat cttacttcta caagatagaa
     8341 ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga aaaaatggag
     8401 acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat
     8461 ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa
     8521 gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac tacttattgg
     8581 gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag ccaaacaggc
     8641 gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag agttgatttt
     8701 tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt tacttgagtt
     8761 tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca tctggtgtcc
     8821 actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga gcatttatca
     8881 ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat tttgatgttc
     8941 ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat tatctagagt
     9001 atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt ttttccattg
     9061 ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa agtgggttta
     9121 atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca caactagctt
     9181 acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt tctaagccgc
     9241 taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg gctgatatct
     9301 tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata
     9361 tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc ggcattggcg
     9421 gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca cgtttgaaac
     9481 cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca tttgaacatc
     9541 tgctatgtgg cgcaagcatg ttacagattg gtaccgagct tcataaagaa gggccggaaa
     9601 ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga tatacgtcaa
     9661 ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa ggtcatgatt
     9721 gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat
     9781 tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata atatgaatga
     9841 tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag aactagttac
     9901 ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga
     9961 accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag ggccattggc
    10021 tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc tagctacttc
    10081 aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg tgcggcaagc
    10141 agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa
    10201 tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg aatgtacacc
    10261 gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc ctttagctgg
    10321 tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat tgattgggct
    10381 gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat ggcaaggagc
    10441 tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat tttatgggat
    10501 gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc aaacacatcc
    10561 aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact cacagattag
    10621 tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag aagggcatga
    10681 tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt attatcagat
    10741 gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc agcgagctgt
    10801 cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc agaagctctc
    10861 tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa
    10921 cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg cccctaaaat
    10981 cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca gtgattgttc
    11041 tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga agatcacacc
    11101 ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta ctcatttgac
    11161 aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag aagatttgct
    11221 atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa atccatggaa
    11281 atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct aataactagt
    11341 gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga taacagctgg
    11401 agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact gtttcccgca
    11461 tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc aaaagaggtg
    11521 gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg acaaatcact
    11581 tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac aaaagaaaga
    11641 gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat gatcgtacga
    11701 aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa agcagctggc
    11761 gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt agtcacatat
    11821 gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac agatgttatt
    11881 ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat ccctgtaaaa
    11941 atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa agaaggaaat
    12001 gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca
    12061 aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg
    12121 gctgagtcgg tgagtgagtg atgtactttc ctctaattga tttttccctc atgtggactt
    12181 cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc gtgtcgtttc
    12241 ttttaggaaa tctggtaggg ttgcttttga cagttttagg tcttttggat tggctgcctc
    12301 tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca ctagtcctgc
    12361 tgtttctgtt atattttggg ttaccttatc aattaagtgc cttaacggct tcagtcatct
    12421 gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca ctagcaggcg
    12481 tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt ggggtattga
    12541 aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt aacgtagcta
    12601 tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa atatttcaaa
    12661 aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt ttagttgcac
    12721 tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta gaaaaacgtt
    12781 ctatgcatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca ccatttctat
    12841 tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc
    12901 gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa tagaatcctc
    12961 gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca cgatgcctga
    13021 tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa gccgagaatg
    13081 gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca aactgtcatt
    13141 tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag taaaaactga
    13201 aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag aggagatgcc
    13261 tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga
    13321 agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa aagcaaagca
    13381 aatggagcga ggcgtaaaat attttccaac agatcaattg tacaaagaat tgaaaagacg
    13441 ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt tagaggaaat
    13501 agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc atgattatga
    13561 atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg agtgaaaaat
    13621 atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa agaagcactg
    13681 tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg tgattccgct
    13741 acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc tgtgatccaa
    13801 aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg gacagtcaaa
    13861 gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag tgaagtactg
    13921 gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga tacagaggtg
    13981 tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag tcgctttcgc
    14041 atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt
    14101 gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt tttccacgca
    14161 gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt cgataaaggg
    14221 ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg caatatacgc
    14281 gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt gaagtttctt
    14341 gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact cttgattact
    14401 tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg actgggagtg
    14461 ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct agatcatcca
    14521 aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat ccaatgatta
    14581 gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc ggcaaacaag
    14641 gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt tcattgcttc
    14701 gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga ttttgaacga
    14761 tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg gaacagctgc
    14821 attagtctca agcgtggatc ccgtttacac aaaaatagat ggtagttgga aagaatcaac
    14881 tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact
    14941 agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata tccaagatat
    15001 ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt ctgctggatt
    15061 cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag catatcaaat
    15121 aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca ctactgaaaa
    15181 aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa aaaacaagaa
    15241 agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt acgaaatagc
    15301 tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac gacctctcgg
    15361 cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt attttatctg
    15421 cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata gctttcaaac
    15481 gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc tgttcagatg
    15541 aatctccacg ttttttcagg cgcgattgca atgtttcttt agagatcgta ataaagatag
    15601 gacggactac cgtcccgaaa tactcatata aatgccaaaa accatttgct gtcaaaacgg
    15661 cgtaacaatt tcctactgtc agtttctttt taatttctgc tttactggaa ccatatttat
    15721 gttgagcata ttggtcccac tctgcaaact catttccctt gatcatctga ttgaatgtat
    15781 tgtcagaaac aaaataataa tcctcttgat ctttttcccc aggtcttggt gatcgattcg
    15841 tgtaagtaat gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa gtttttccag
    15901 aaccagaagg accgataaat acatagcaat aattcattgt catttctcct ttgatgattg
    15961 aacaagtgaa aaagctttgg aaaatatttc caaagctttt aaaagtaact ttgctttttt
    16021 gtatattata ccagcaagcc agtcacaata gcacttaaaa agctgactaa agtcgctgtt
    16081 agcaaaattt tcaatccgtg ttttgcgaca agatttcctt tttcttcatt taatcccttc
    16141 atcgcaccag aaatgatccc gatagaagag aagttggcaa atgaaacgag gaagacagat
    16201 ataattgctg ttgtacgctc agtaaaatgg aaattaccat ttgctaattc tgtcatagca
    16261 acaaattcat ttgtaaccag tttagtcgcc atgatactac cggcatccac tgcttctttc
    16321 catgggattc cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa
    16381 gtgatgccga atatcccgtg gaagatagca ttgatcatgg caatcaaagc aacaaaaccg
    16441 ataagcatag cagcaaccgt gatcgccaca tggaaaccat caagaatgta ttctcccaac
    16501 atttggaaaa atgtctgttc tttgttttct tcaatgatca gctcatcttc tttttcttct
    16561 aatacatagg gatttacaat tgaagccaga atataaccgc caaataagtt caaaacaagg
    16621 gcagtgatca catatttaga atcgatcagt gccatataag atccgacgat cgacatagaa
    16681 actgtagaca tagccgatac actcatggtg aacaaacgtt tctcagatag gaagggtaat
    16741 tcctttttta tggaaataaa aacttccgat tgtcctaaaa tagcagaagc tactccatta
    16801 taagattcca atttgcccat cccgttgatt ttacttaatc ctagaccaac agctttcata
    16861 aataatggaa gaatcttgat atagcgtaga ataccgataa tagcagaaat aaatacaatt
    16921 ggcatcaata cggaaagaaa gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca
    16981 aagtcaacac cttgcccagc aaaagcaaga agatgatcaa acatttttgc caatacagaa
    17041 acaaccgctg taccaaaagt tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg
    17101 aacatcacaa ttagtggttt caatttgatt gcttttttgt tactgctgat caaaaaagca
    17161 agccctaatg tgacaaataa accaataata cctaataaat aactcaaata atcactccta
    17221 ttccaaattc cgttttattt tacctgtttt gcaaataaaa tactaatctt tagtgaaatt
    17281 ttcattactt ttttagagtg aaacgcattc attttccatt agaaaataaa gcgaacaaaa
    17341 taaaaggttt aaaaagtgta acgtcttagt aaagtctata aaaaaacgaa actatcgatt
    17401 aagctaatag tttcgttttc aacatttata agtaaacttt ttctattggt aatatagatt
    17461 ttcggaaggg tagacaggtg cgcaggtaat atcgattcct agttttttca atgtttgttc
    17521 atcattttta ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt cagctagttt
    17581 atcataagca acttgagcga ttggattcgt tactgcactg attgataaag cgatcaagac
    17641 ttcattgatg cttagtgcat ccaaagagga atgaagtgtc tttgatttca atccttgtat
    17701 tgtttcaaga actaatggtg acaataaagg aatgtcatct gcgatatggg ctaaatgttt
    17761 tactgcattt aagataactg ccgctgacgc atccatcaat gaacttgtac gcccggtaat
    17821 gatgacttca tctggtaatt cgattgcaat cacagcagaa ggatcttttg taggactttg
    17881 cgcttgaatc ttatctgcat agtcatgggc agggactacc acgtttcggt cttcaggacg
    17941 taaaccgact tcttccataa tcagtttgat tcgattaact gcttcttcgt cgatcaatcc
    18001 ttttttgaag tcgcattctg tttggaaata acgacggatg atttcttgtt tagaggcttc
    18061 ttggacaacg gtatcatcca cgatcccaaa tcctacacga ttcacaccca tgtcagtagg
    18121 agattgataa acagattctt taccagtaat tttttcaata atacgtttga ttacaggaaa
    18181 tgtctcaaca tcccgattat agttcacagc tactttattg taggcatcaa agtggaagga
    18241 atcgatcata ttgacatctt ttagatcgac tgtggctgct tcataagcaa tgttcagcgg
    18301 atgtttcaac ggaacattcc atactgggaa agtttcgaac ttggagtaac cagctgcatg
    18361 tcctttttgg ctttcatgat acagttggtt caaggaagta gcgagctttc cacttcctgg
    18421 gccaggagct gtcactacaa cgattggttt agttgtttca atatactcat tttttccaaa
    18481 accatcttct gagacgattt tttctacgtt gactggatag tcttcgattt ctgagtgttt
    18541 atagaccttg atgtttcgtc tttctagttt attgataaag actttcgtgg caggctgtcc
    18601 actatagcga gtgataacga cactgttgat taataagcca taaccacgta attcatcgat
    18661 taaccgcaag atatccatat cataagtaat cccataatca ccacggatct tattacgttc
    18721 aatatctcca gcatagacgc aaatcagtat ttcagcttga tctttcaatt tttgcagcaa
    18781 tttgattttc gcatcttctt caaatcctgg aagtacacgt ttcgcatgct tgtcgtcaac
    18841 tagcttccca ccaaattcta aatataattt gtcatagtgg ttcactcgtt ccaaaatata
    18901 cgctgactgc tcttcaatat atttttctga atcaaaacca atttttttca ctaggttccc
    18961 tccttaagta ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg aagagcgaaa
    19021 ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt ctagatagtt attcattggt
    19081 atgatagttt ttgataagga aggtgctaca tgaaaaaaag acagaaaaag aaaaacgcat
    19141 ataaacacta tatccgttcg atttttaccg gttatgaaag aatgcttgaa gatcccgaat
    19201 tggaacaatt gacgtttact tatctaaacg aagaaacgca gctaactcgt gatgagcatc
    19261 agcgcattca tttcacaaca agagatctgc ctagtaaata aaaaataaga taaaggtcat
    19321 cggattcgat tctgtcctat cagatggaaa gactagttga tcgaaaaaaa ggctgtacca
    19381 aattttggtg cagcctgttt tatggagcat tgcccgctct tttttataac aattttttac
    19441 agaaaatggt actattgaag tattaataag tgaaaaacag atgagtaaga ggaggcaagt
    19501 agtatgaaag agccagatac aaatgcaaat tatgagccag acgaactaga ggagacgaat
    19561 gcggatcagg atacaggaca agaatcgtct tctttttacg atatcaaaga aaaagcaagc
    19621 atgaatcttt cccaattatt agaagactta caggcttttg aaaaagctgt ccgcgaagaa
    19681 gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt aaaaagaagc
    19741 tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga tcgtttttta
    19801 caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag ctattacaaa
    19861 atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct gccagaaatt
    19921 tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga gtcaacggac
    19981 tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac agaaattgaa
    20041 cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa cgacaataaa
    20101 ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa gaagaagcaa
    20161 gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga aacaatccag
    20221 cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc agccatcgta
    20281 gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt cggtcaagca
    20341 atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca atctaatgaa
    20401 ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg aagaacaaac
    20461 agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc ctctggatga
    20521 cacagaagaa atccctatgc tagaggaaac aaaaatacac gaaaaagaaa aagtaactcc
    20581 gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa atcccgggta
    20641 tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc
    20701 agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc cacaattatt
    20761 agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg gaaagacaag
    20821 cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg gtctggaacg
    20881 gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt atacgttgaa
    20941 tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac gataccaagt
    21001 ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg tccaagatga
    21061 ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg cagggcagat
    21121 cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac ttggttttac
    21181 cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc agagcgaatt
    21241 tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa atccatccta
    21301 cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag gacagtcttt
    21361 aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg gagactaccc
    21421 acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa tggtgagaaa
    21481 gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt ccaaaggact
    21541 tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa atggacgtga
    21601 ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa tagcacagct
    21661 tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta ttccgaattt
    21721 ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa aagatgcgtt
    21781 aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg gaccatgggt
    21841 catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg tctgcttgag
    21901 aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg cgggcatggt
    21961 ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc aaccttggat
    22021 ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg ttgtgttctt
    22081 tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa
    22141 aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca agttgcagta
    22201 caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa
    22261 caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtgttc ttcaaaaaag
    22321 gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat aacggcttca
    22381 tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg atgtttactg
    22441 tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat ggacaatcgg
    22501 tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg cgaatattgc
    22561 cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa
    22621 gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct atcagcaaat
    22681 ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg
    22741 ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat aaaggacaga
    22801 gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca atcgtcgcaa
    22861 tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa aagttgacgc
    22921 atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta tttggtttgg
    22981 taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa
    23041 aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg attattttta
    23101 cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca atattgactt
    23161 atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt ttcttgctga
    23221 tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt gaaagcgtga
    23281 cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc agtcggctga
    23341 tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca aaagcagata
    23401 tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa aaaaatatgc
    23461 gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa tatgacccaa
    23521 attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga aaaatataat
    23581 cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga gtccgctgtt
    23641 tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg ccatggcttc
    23701 aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga ttagcttgcg
    23761 gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta tcgattctgg
    23821 tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa gcatcatttg
    23881 caacgacatt attgatggtt ttctttttag aattacgcta tacattatta ggagcaagtc
    23941 ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct caatcactga
    24001 atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc tggaataaaa
    24061 gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc aatatgatcg
    24121 gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt gctttgacgg
    24181 ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc tttacaggtt
    24241 tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact ttcggattga
    24301 ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca ttcaaaaaag
    24361 aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc ttcaacttcc
    24421 cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt tgcttgtctg
    24481 ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca tcgaaaagtg
    24541 ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc ggcgttagct
    24601 tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat caaaatagct
    24661 gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt gtcagttttg
    24721 acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt ttattaaaaa
    24781 cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg caacagtcaa
    24841 ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta tgatgcaagc
    24901 tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt gctataatag
    24961 ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa aaaattgatg
    25021 caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg ggtagtctat
    25081 ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc tacgacattg
    25141 tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt cggatttcca
    25201 aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc tgcttgtcgg
    25261 ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc tgctgcactg
    25321 atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt taataatggg
    25381 atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct tagtgcaatg
    25441 agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt gattatcgaa
    25501 caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt attgactgac
    25561 ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg tgggttagct
    25621 gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact taaagatttt
    25681 ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc tatcattcgg
    25741 gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat gatcgaccaa
    25801 gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca gcaactggaa
    25861 ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct gatctccatg
    25921 gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa aaaagatcgg
    25981 atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat tcttcttcct
    26041 tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt tggctatgat
    26101 cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt ctctttattc
    26161 acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat ccgattaact
    26221 gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt agttggcgga
    26281 tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg cggctatcac
    26341 tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt tttcttaaaa
    26401 actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt tttgctgaat
    26461 cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc tgtaggaagt
    26521 attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg aatgttgaaa
    26581 aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt tgctgtcact
    26641 tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa ttttagtttc
    26701 atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg aaacgaggaa
    26761 attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc ggaacgtatc
    26821 aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg gaaaatttat
    26881 aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa cagtaaagat
    26941 tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga ggaattgaaa
    27001 gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc tggctttgtc
    27061 aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc ttatgactct
    27121 aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt aaatgatgaa
    27181 ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa tacctacgct
    27241 tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac aaatggtgca
    27301 gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga agggaaaatt
    27361 gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga agaaattatt
    27421 ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca tgcaaataat
    27481 ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa tgcagaaatc
    27541 gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc catcggcatt
    27601 gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaatat tccaacatca
    27661 tcttggactt tctacatgtt actaatgagc gtgtaaaaga acgagatggt gtttttattt
    27721 ctatcaatga agctcttttt ctttgcaatc ctcacttcaa tcctctataa tgtcgcatat
    27781 agtttagtaa atgagtaaac tactaattta cttatttact aaaaataaaa ggatgaggag
    27841 ttaaaataat ggaaatacca acaaatttaa aacataaacc agtaatcgtt gtagaagact
    27901 atgataaagt tgatggaaga aatgcattaa atacggatgc aaaagggctt tcactgggcc
    27961 ttgctcagtg gaatgatcgt ggaaaagtcg atatctcagg caaggtatgg cgacatactg
    28021 gagaaaaatg gtcacgacag tcggaagaat taccaatcac acgtatttta gatttagcga
    28081 tcttagctgc acaaggaagt ttatacttcc aagatgctta tcgttatgaa aaattttatg
    28141 atccagaaaa tccagtcgta gacatcattg gattacaagg gaaccgaatg acggtagaag
    28201 tagataccaa aaatccaaag atcgatgaag acatcatttt gtattacgat acactacaaa
    28261 aagacggaga actgattggg gaacgattcc gtattttaaa acgtctttta gatgagctag
    28321 gatattaata gacagagatg atcaaaaggg gaagagaagc atggagaatt tctcactaac
    28381 agaaaaccga accttttcac ggctagaaaa gcaaatgatc tggaagaaac cgaaaagcca
    28441 tcggacaagt gcagaagaat ggcggatcgc ctccgaaatc aaagggaatt ggcaagatcc
    28501 tgaaatgaaa atcagtaacg tacttttgga aggagatgcc gggtcgggga agacacaatt
    28561 agccaaagca ctatctgctg atctgcaact tccctataca aaagtgacct gttttgcaga
    28621 tatggacaaa tcagatgttt tcggagcctt attacctgta gtagattcta ataaggaaca
    28681 agaccaagaa ttactagaag ctatttacca aacagataca cttgaagctg tactgacact
    28741 gatccagaac cattacggca ttgatccttt ttctgctaaa gaaaaattag cagagctagt
    28801 tcaaaggatc gaagctgatt atccagatgc aatccattac agctattacc cttctgaaat
    28861 cgttcgagcg attgaaaaag gatatttatt agagattcaa gagccgactg tcatccgaga
    28921 tgcctctgtt cttgtggcac ttaactcagc gctcgaacca aatgggatgt tgaatttacc
    28981 gacaggaatc attcgtcgcc atccagattg cgtcattgtg atcactacaa atcgaaatta
    29041 ccaaggaaat cgcccattaa atgaatcttt aagagatcgg atgcaacatg cggaaaaaat
    29101 ggatcttcct gccttagaag tcatggcaga acgtgcgatg gcgaaaacta tgatccaaaa
    29161 acaagaactt ctcttaaaga tggcagagat tatccgtttg ttggatgaaa ctgcaaaagc
    29221 taacgccatc aaaggtgtag caggaatgcg ttcttatttt tattgggcaa atacctataa
    29281 gcaaggacaa gatttactcc agtcgattta tccaaaagtt ttgtataaat tatccactga
    29341 ttcagatgaa ttggcaattc ttgaacaagc tttgtctgac agtggattgc tactggaact
    29401 tcaggacata ttgagaaaag aaaaatgggg tcatcctcaa tctgagcata caaaaggaag
    29461 aaccattagc gccgaagaag caaatgaacg aaacatcact gaaaatgctg aagaaattct
    29521 gcgcttagat gaaaagaaat cgttgtctgc tgaagaacca aatgagggag aagagaaaca
    29581 agaagaaaca atagaaaaaa acgagattcc tcctcaaatg caaaaagaac gcagtagcga
    29641 tatcgaagga aaaaatcaaa aagaacaaca aacaggagat gattctcaag aaatgtctgc
    29701 ttctgacatg gaagcacatg ataaggaaat caagaagcaa ctgaataaag aagcgcgaca
    29761 aatcatgaag gaaacgattc atgaaaaaga aggtctcatc gttcaccgac cagctttcca
    29821 aggaaaaacg ccggaagcac gtgagctaca catgcaagta atgccaatag tagaatcact
    29881 tagccggcaa attttagagt tactggataa tgaacaaaca gagacgtacc aaaaaggaaa
    29941 atacgaaggt cagcggttca atgcaagcaa agtagcatat ggtgatttga gaaattttga
    30001 caaaaagaac ccgccacatg agcaaccttc tttagcggta gctgtacgga tcgatgaatc
    30061 tgggtcaatg atacgggatg atcggatcga agcggcaaaa aaagcagcga ttgccattgc
    30121 cgaattttct aagaaagtcg atatcccatt gttgatttac ggagatacag cagaccgttc
    30181 gtcaagagaa aagacttctt tgttttctta taaagaattt gaagacggat ttcaatggtt
    30241 aaatgaaaaa ttagtcacta tgaaaccaag gcaaaacaat cgtgatggtg ctgcacttcg
    30301 tttagcagca gaaaaattaa acagacaatc cgcaacaacc aaactgttgc ttaatattag
    30361 tgacggtcag ccaaaagcgt tgccggatta tacaggcaaa aaagcaaaag aagatatcca
    30421 gtctgttttg aaagaatatg aacgtcaagg aatcctattt gtctccgcag ctattggcca
    30481 agacaaagaa gaaatcaagt cgatctacgg tgaaagtcgt tttgtagata ttactgactt
    30541 gaatgaattt cccaagcaaa tgattcaact gattgcccgc tatctatgat aaaataaagc
    30601 tcaacgaacg cacttagcag attcgttgag cttttcgttt ctagttaccg gtaacaagga
    30661 tacttctaaa atagaatcgg ttataatcaa gagggaccgt taaaggagtg aaacgatatg
    30721 atacaagaac aaaaatggtg gaaagaagca gtaggctatc aaatctatcc tgctagtttc
    30781 aaagatagca atggcgatgg tcaaggagac atcaatggga tccgtgaaaa acttaattat
    30841 ttgaaagatc ttggtatcgg ctttatctgg atcaatccga tctatcaatc tccatttgtc
    30901 gataatggtt atgacgtaag tgactatcaa gatatagaag agagattcgg aacgatggaa
    30961 gattttgatt tattgctaaa agaagcacat gaattaggga tcaaagtgat tatggatctt
    31021 gtcatcaacc atagttcgga tcagcatcaa tggtttgaag aatcgaaaaa atctaaagaa
    31081 agcccgtatc gcgattatta catttgggtc gacggggtag atggcaaaga accgaataat
    31141 tggacatcga ttttcggcgg ttctgcttgg gagtattctc atgaaactgg acagtattat
    31201 ctgcatgttt ttgcaaaaga acaaccagac ttaaattggg aaagcgagaa attgaaagaa
    31261 gaactattca atatgatccg atggtggttg gataaaggga tagatggatt tcgtttagat
    31321 gcgatttctc atgtaaaaaa agatgaatat tcagtaaaag ccacagagaa ccctttctca
    31381 ccctttcaaa atgtctcagg aatcgaagaa catttgaccg atttgaaaca tgtttttgaa
    31441 gagtacgata tcatgactgt cggagaagca agtggtgtca ctgcagaaga aggaccacaa
    31501 tgggtaggga aagacggtta tttcgatatg atattcgaat ttgatcatat ccatatctgg
    31561 cagcaagaaa aagaagggca attagatgtc cttaaattga aacttgcatt aagcgcttgg
    31621 caaacttcat tggatggtat cggatggaac gctttatata tggaaaacca tgatgttcct
    31681 cgtgccgttt cagtgtttgg agatactcgt cccgattttt gggctatgtc tgcaaaagca
    31741 atcgctatga tgtatttctt tttgcaaggt acgccattta tttatcaagg acaggaaatc
    31801 ggaatgacga atatgccctt tgaatcgatc gatcaagttg atgcagtaga ttcgaaaaga
    31861 ctttataaaa ggctgcttgc ggaaggtaag acaagagaag aagcattgga tatcattcgt
    31921 gaaacaacga gagacaacag caggacaccc atgcaatgga cttcggaaca atatgctggt
    31981 ttttctacac atgaaccttg gctgatcact aatccaaata ccaaaacgat caatgtagaa
    32041 caacaagaat atgaaccaga gtcggtcctg caattctata agaacatgat ccgcattaga
    32101 caaaccaata aaggtctgat atacggttct tataaagaat atcttcatga acatccacaa
    32161 ctttatgtct atgaaagata tttagaagat gaacaatatc ttattatggt aaacttaaca
    32221 gaatcacttg ctgactatga acttccaaaa gaagcagatc agtcctggac attactcttg
    32281 tcaaattcct ctagcggaga atttgaagct aaaggcatac tagcacctta tgaagctcgg
    32341 ttatataaaa caaacaagta aaacttcctc gttaaagttc attagttgga ctttgcgagg
    32401 aagtttttca gattgctctg tcaattactc atttcaataa ttcgctctag gaccaccgat
    32461 atatttagga cgagagcgaa gggctgtcac gtgagcttgt ccaagggttt tcaaacgagg
    32521 tctgacaggt acttggacat gtttgatatg cataccgatt gatgtgtcac cgatatcgat
    32581 accggcttgt ccaactacgt gttccacttc gacaggatca ttgaactggt caaaagcagc
    32641 aacagaacaa gcacctccag catgtagtgc aggaacaaca gagacaattt cgaaattttt
    32701 tgcttctgcc actgttcgtt ctactactaa tgctcggttc aaatgttcac aaccttggac
    32761 agctagcgca atttctttag gatccaacaa ctcttttaac gtacggatga tccattgacc
    32821 aacttccgca ctggaatttt ttccaatatg tcctccaacg acttcgcttg tgctgcatcc
    32881 taaaacaaaa agatcacctt ttttcaagtg tgcctcagcc aatatgtcat taactatttc
    32941 agtcaattgg tcttttaatt ctttttctga caacttcatt ttctaaaacc ttctttttca
    33001 attaatattg cttccgtgct tgaatacgtt gaagacttgc agttatagga aaggcaagtc
    33061 ccattccaat cagattttgg aagatgtttc ctggtataga agccactcca gccggccaat
    33121 catataaaat ccaaccggcg ataccatagc cgatgatcat aactaagctt cctaataaaa
    33181 gccctaatcc ccacatacga gtagaagatt tgctgaaaga accagcaaca tatccttgta
    33241 acccgtggat cactaatgaa aaaactgccc actgcggata tcctgaaatc aaatcgatca
    33301 atagaccact ggaagcacca acaactaatc cacccgttgg accataaaga aaagcagcgg
    33361 tatagatccc ggcctcgcat aatgtcacaa atccattcgt agccggtaca gggatcaaaa
    33421 ttgttaatga aagggcaact gtcaacgcaa tcagcatagc atagatcgtt aattgcttag
    33481 ttttcatttc tcattcctcc tttttgaata ccgccagacc agacacttcc ggtatgatcg
    33541 ttcatcagca cgccattaga aatacaatgg gcaacaaatt ttttactttc tgctactgct
    33601 ttggtaaaat ctgcccctaa cacaagattc gctgttattg cagaagaaaa ggcacaaccg
    33661 gccccgttta ctgttgtagt gtcgattttt ttatgagaaa atacctcata atgatcaatt
    33721 gaatagagaa gatccacagc ttcatctcca gccatccttg tgccgccttt gatcacaatg
    33781 ttttttgttc ctgaagcagt gagttcttta gcgagatgga tcatttctgt taacgaagag
    33841 ggaagagctt gaccagaaag taaagcagct tctgccaaat taggcgtaat catcgtaaca
    33901 agtggaaaaa gttcttcgat gagctgcgaa atatacaatt gattgatcac agcttcagtt
    33961 tctttgaatg ccaaaacagg gtctaaaatg attgggatat tttgttttgt ttttatgaag
    34021 tctcgaacaa ccatgattgc ttcacttgaa ttcaataacc caattttgat accatctaat
    34081 gaagtcattt tatcaatcga aactaactgt cgtttaagca attccaaaga tatagattcc
    34141 agcataaatc gctcattaat atctagtgcc cctatagaag tcaaggcgct tagtccgaat
    34201 agaccaaaat tctcaaaagt ttttaaatct gtttggattc ctccaccagc aaatggatca
    34261 gaaccgccaa ttgtcaagat tcttttcata acattttcct ccatgaataa gagtataacg
    34321 aaattttcaa aaaagaaaaa cagccaattg gcatattttt atctcatcca atttaaaaaa
    34381 acaaaaagaa aagaaagacc aaaatggatc tttctttcct taaatagtta ggacttttgt
    34441 gattttaatt ctttctcgat ttgaagccgt tgcactttca ttgtagctgt tcgcgggatt
    34501 gcatcaaaat cacggatgat agggacgttc aagcgaggaa gttcatggac ttgctcccac
    34561 catcgattcc aatccatttc tttatcagga gctaaagcaa taatcggttg tggagaatga
    34621 tttttatccc ttacaatcac tacttctgcc aaaaaatcta acgaatctaa taaaaagtct
    34681 tcaatagcta atgtactatt gatcgtttcg atcaaatcca cttgccgatc tttcaaaaat
    34741 aggtggccat gcttatccat aaaaccgtaa tctccgctat cccaccaatc gccataaaca
    34801 ttttcctgga accgtgcatc ttccttgtaa taagtcaaag cccgtccttt agacagtaat
    34861 tgtatatgac catctgtgtt ttcaggtagg acattcccaa ctgaatctgt aatccgagcg
    34921 cttgtcatgt cttccagtcc aacgcccata tctcttgcat cacttgtttt caaactctgt
    34981 agtgtatgag ccttaagaat cattggtccg cattcacttt ggccatatac ttgaagaaat
    35041 accgcatcct gtgccatcga cgttcgcaaa aatgtcttca ttgtctggtt gtttatagca
    35101 tcaaaagtgg agtgatagta tcggataccc gaaaaagctt ctggatgctc cttagcagta
    35161 aaacgccact gaacaaagtt attcggatgg gtctctagtg caattggccg gtgagcttcc
    35221 aacatatgaa caacttttga actttgtgcg tttgctaaag gcatcatagg aaatcccatg
    35281 gacatcaaag aagagatacc aatattgaat ctggaatgca ctggagagat gtgaaaagcg
    35341 attaatttct tttccgaaat ttttgtgaaa actgtttttt gccatttcgt ccgccatccc
    35401 atagaatgag cagaatgaca aataagttta gggataccag ttgtaccaga agtatgggtc
    35461 atataagaaa tttcattttt gtctaaaaat gattgagaaa cagacaaagc aggttgttcc
    35521 attaaataaa gaataggaa
//
LOCUS       DANKMN010000022        34962 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000022 DANKMN010000000
VERSION     DANKMN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="PW224_001056"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="PW224_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL2907763.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="PW224_001057"
     CDS             complement(2020..2922)
                     /locus_tag="PW224_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2907764.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="PW224_001058"
     CDS             complement(2919..3611)
                     /locus_tag="PW224_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL2907765.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="PW224_001059"
     CDS             complement(3654..4295)
                     /locus_tag="PW224_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2907766.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="PW224_001060"
     CDS             complement(4333..4647)
                     /locus_tag="PW224_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907767.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="PW224_001061"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="PW224_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="PW224_001062"
     CDS             complement(5984..6916)
                     /locus_tag="PW224_001062"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL2907768.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="PW224_001063"
     CDS             complement(6913..7689)
                     /locus_tag="PW224_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL2907769.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PW224_001064"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PW224_001064"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL2907770.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PW224_001065"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PW224_001065"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL2907771.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="PW224_001066"
     CDS             complement(12014..13294)
                     /locus_tag="PW224_001066"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL2907772.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="PW224_001067"
     CDS             complement(13313..14239)
                     /locus_tag="PW224_001067"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL2907773.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="PW224_001068"
     CDS             complement(14252..15535)
                     /locus_tag="PW224_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL2907774.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PW224_001069"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PW224_001069"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL2907775.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="PW224_001070"
     CDS             complement(16323..17234)
                     /locus_tag="PW224_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2907776.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="PW224_001071"
     CDS             complement(17231..17695)
                     /locus_tag="PW224_001071"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL2907777.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="PW224_001072"
     CDS             complement(17700..18398)
                     /locus_tag="PW224_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2907778.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="PW224_001073"
     CDS             complement(18385..18771)
                     /locus_tag="PW224_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907779.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="PW224_001074"
     CDS             complement(18918..20588)
                     /locus_tag="PW224_001074"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL2907780.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="PW224_001075"
     CDS             20771..21004
                     /locus_tag="PW224_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL2907781.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="PW224_001076"
     CDS             complement(21103..21534)
                     /locus_tag="PW224_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL2907782.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="PW224_001077"
     CDS             21613..22026
                     /locus_tag="PW224_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL2907783.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="PW224_001078"
     CDS             complement(22064..23212)
                     /locus_tag="PW224_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2907784.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="PW224_001079"
     CDS             complement(23334..23930)
                     /locus_tag="PW224_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2907785.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="PW224_001080"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="PW224_001080"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL2907786.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="PW224_001081"
     CDS             27160..27591
                     /locus_tag="PW224_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2907787.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="PW224_001082"
     CDS             complement(27636..28985)
                     /locus_tag="PW224_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2907788.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="PW224_001083"
     CDS             complement(29055..30218)
                     /locus_tag="PW224_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2907789.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="PW224_001084"
     CDS             30347..30670
                     /locus_tag="PW224_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907790.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="PW224_001085"
     CDS             complement(30853..32202)
                     /locus_tag="PW224_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2907791.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="PW224_001086"
     CDS             complement(32271..33224)
                     /locus_tag="PW224_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL2907792.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="PW224_001087"
     CDS             complement(33326..34516)
                     /locus_tag="PW224_001087"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL2907793.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9952 a         7642 c         6120 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DANKMN010000023        34771 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000023 DANKMN010000000
VERSION     DANKMN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(227..1114)
                     /locus_tag="PW224_001088"
     CDS             complement(227..1114)
                     /locus_tag="PW224_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2907794.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="PW224_001089"
     CDS             complement(1111..1980)
                     /locus_tag="PW224_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2907795.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="PW224_001090"
     CDS             complement(2117..3403)
                     /locus_tag="PW224_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2907796.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="PW224_001091"
     CDS             3676..4254
                     /locus_tag="PW224_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2907797.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="PW224_001092"
     CDS             4254..5183
                     /locus_tag="PW224_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2907798.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="PW224_001093"
     CDS             5180..6916
                     /locus_tag="PW224_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2907799.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="PW224_001094"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="PW224_001094"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL2907800.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="PW224_001095"
     CDS             complement(7812..8321)
                     /locus_tag="PW224_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL2907801.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="PW224_001096"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="PW224_001096"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2907802.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="PW224_001097"
     CDS             complement(9622..10713)
                     /locus_tag="PW224_001097"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL2907803.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="PW224_001098"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="PW224_001098"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL2907804.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="PW224_001099"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="PW224_001099"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL2907805.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="PW224_001100"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="PW224_001100"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL2907806.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="PW224_001101"
     CDS             complement(14676..15650)
                     /locus_tag="PW224_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL2907807.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="PW224_001102"
     CDS             complement(15631..17514)
                     /locus_tag="PW224_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL2907808.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="PW224_001103"
     CDS             complement(17532..19757)
                     /locus_tag="PW224_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2907809.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="PW224_001104"
     CDS             19872..20906
                     /locus_tag="PW224_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2907810.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20973..21986)
                     /locus_tag="PW224_001105"
     CDS             complement(20973..21986)
                     /locus_tag="PW224_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2907811.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22064..22252)
                     /locus_tag="PW224_001106"
     CDS             complement(22064..22252)
                     /locus_tag="PW224_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL2907812.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(22692..22820)
                     /locus_tag="PW224_001107"
     CDS             complement(22692..22820)
                     /locus_tag="PW224_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2907813.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(22823..24196)
                     /gene="rlmD"
                     /locus_tag="PW224_001108"
     CDS             complement(22823..24196)
                     /gene="rlmD"
                     /locus_tag="PW224_001108"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2907814.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(24307..25362)
                     /locus_tag="PW224_001109"
     CDS             complement(24307..25362)
                     /locus_tag="PW224_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2907815.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(25372..26802)
                     /gene="gatB"
                     /locus_tag="PW224_001110"
     CDS             complement(25372..26802)
                     /gene="gatB"
                     /locus_tag="PW224_001110"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL2907816.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(26799..28271)
                     /gene="gatA"
                     /locus_tag="PW224_001111"
     CDS             complement(26799..28271)
                     /gene="gatA"
                     /locus_tag="PW224_001111"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL2907817.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(28271..28576)
                     /gene="gatC"
                     /locus_tag="PW224_001112"
     CDS             complement(28271..28576)
                     /gene="gatC"
                     /locus_tag="PW224_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL2907818.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(28682..30718)
                     /gene="ligA"
                     /locus_tag="PW224_001113"
     CDS             complement(28682..30718)
                     /gene="ligA"
                     /locus_tag="PW224_001113"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL2907819.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(30735..32969)
                     /gene="pcrA"
                     /locus_tag="PW224_001114"
     CDS             complement(30735..32969)
                     /gene="pcrA"
                     /locus_tag="PW224_001114"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL2907820.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            33124..34683
                     /locus_tag="PW224_001115"
     CDS             33124..34683
                     /locus_tag="PW224_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL2907821.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         9518 a         7494 c         6336 g        11423 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
    20941 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
    21001 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
    21061 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
    21121 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
    21181 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
    21241 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
    21301 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
    21361 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
    21421 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
    21481 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
    21541 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
    21601 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
    21661 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
    21721 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
    21781 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
    21841 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
    21901 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
    21961 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
    22021 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
    22081 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
    22141 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
    22201 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
    22261 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
    22321 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
    22381 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
    22441 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
    22501 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
    22561 attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat
    22621 aatgtagact aactaaaaag actaagaaac aataacaaaa gttaacactt cttagtcttt
    22681 ctttagcacg tctatttatt aaagatttta gggccttttc tcatgttttt acgatcattc
    22741 ttaggtgttt ccatttgttt ggatcggcct ccaccttgtt tctttttgat caattctttc
    22801 attttttctt tttggtccat ttttattctc ctttaacgaa tgaagcgact aattcgatat
    22861 gatgggtttg cgggaaaaga tctacaggct gaactttctt cacggcataa cctgcttctg
    22921 caaataactt agcatctctt gcaaaagttg ccggattaca tgagatatag acgattcgtt
    22981 ctggtgacat ttcgattgct gattcaataa aatgatgatc taatcctttt cgtggagggt
    23041 caactacgat cacagatggt ttgatccctt tttcttgcca ttttttcata actttttctg
    23101 cttttccgac ttcataatga acattttcga ttttattatt taaagcattg aactgtgcat
    23161 cttcaattgc ttctggaata acttccatcc catacacctt atccactctg tctgctaaag
    23221 acaagccaat cgtacctatt cctgaataag cgtcgatcac aatgtctttt ttatctaact
    23281 cggcaaactc gatggctgtt tggtacagat actctgcttg ttgagtattt acttgataaa
    23341 atgactttgc tgagattcga taggtttttc ctaatagctg atcttcgata tagtctctgc
    23401 catataaaac tttttctttg tctcctaaaa tcacatttgt tttttcttca ttgatatttt
    23461 ggatgaccga aaccacttct ggcaattctt catgaatagc ttcagcaatt tccttggatt
    23521 taaagaattt ttctttacga gtaactaaaa cgaccatcat ttcatgggaa taatgaccac
    23581 gacggataat gatgtgacga agctgtcctt catttttttc ttcattatat cctcgtactt
    23641 ggaaccgttg caagatatct ctgactactt tgattgcttg atcgatgaca ggatcttgga
    23701 tataaaaatc ttcgataggg actagatcat ggctattctt tttgtaaaaa ccggtggcta
    23761 actgtccatc gatacggcga acagggatct gtgctttatt acggtagccg aatggttctg
    23821 ccattccgac cgttggaagt acaggaactt ctggcatctt agcaatcttc gccattacat
    23881 gctctacttg tttttgtttg aagacaagct gttgttcata cttcaaatgg gctaaaggtg
    23941 cgatacctat gcgaagtaat gcattgtttt ctacaggctg acgatcaggt gattgtgtaa
    24001 taaatgtttc gacttttcca tatccaaact tattgcctgc ttttactact agtatttcta
    24061 cttcttctcc agggagtgca ttttcgataa ataatggata accatcgatt tttgctaccc
    24121 ccattccttc ataactcaag tcgacgattt cgactgtata tcgttcgttt ttctttacta
    24181 tttgctgttc tgtcatattt tgctccttta agcctatttt tcaatcctac tgaactctac
    24241 tataaatcag ctaattacac aactaaaacc ggcactgacc ttactacgtt cagcaccggc
    24301 ttttgattaa ttcttttgat taggtgtttc gtctgctatt tttccgtcac cgtcgatatc
    24361 ttcatccgtt aaacgttcga cttcttttac gaattctttg ctgacaacct ctaattcttc
    24421 ttcatctgta cctgtgattg cttcatcagg aatctgatcg gtattggcaa agaactcaat
    24481 gtgttgatga agattttgga aatgcatcgg tgcttccccg ccgtattcac catctaagtt
    24541 gatcattact ggtcgattga tatcatcttc tgtttcaacg aacaaactgc ttgttttggt
    24601 atagattatt cttggatcat cgacatgtcg accgccattc agcatgagtg ctgcaatatg
    24661 caagatctca aagatatttg ctgtcttcac aatgattaaa gaaaacttcc catcatccaa
    24721 tttggcatct ggcgcgatct gttcgaatcc tccaacagag ttcgtcagcc ctaggaaaaa
    24781 catcgaagca tttccgatat attccccttc atcatacttc aagcgcattt tgattggctt
    24841 tactcgaggg agcatctctg ctcctttcgc aagataagca agataaccaa aaatactctt
    24901 tagttctgat ggaacttcat aagtcaattc tgtcaaatag ccacctgcag cgatattgat
    24961 gaagtaactg ttatcagaaa ggccgatgtc catttttacg gtttggtgtt tttttatcac
    25021 ttctgctgct gctttaatat tatctctagg aatttttaat gctcgagcat agtcgttcgt
    25081 tgtaccagct gggatgatgg ccattttagg acggtgttca agcggagcga ttccattaac
    25141 cacttcgttg attgtgccat cgccaccagc tgccactact agatcaaaac ctagttctgc
    25201 gactctacat gcttcattct tggctgaatc tggttctggt gtagttgcat aggcgctggc
    25261 ttcaaatcct gcttcttcta atgcctgtag gatatccgcc aaattttttt tcaataattc
    25321 ttttcccgag gtggggttat aaattacgcg tgcttttttc attttcagcc tctatcgttt
    25381 cgctaattca tctttaagta gcttattcac gacaccagga tttgcttgtc ctttagttgc
    25441 tttcatgatt tgaccgacta ggaagccaac cgcacgatct tttccatttt tgaaatcatc
    25501 gacagattgt tggttgttat ccaaaatttc attaatgatt ggcagtagtt tgttcggatc
    25561 agataattgc acccaaccat ttttttcaac tacttcttgg gcttctccgc catttgtgat
    25621 caattcacgg aagacttttt tggcgatttt tgagctgatc gttccatcgg cgatcaaacg
    25681 aatcattcct gctaagttct caggtgtcaa ttttgtctca gctaattcca gtttctcact
    25741 gttcaagtaa gcagaaactt cacccatcaa ccagttagat gcttgcttgg catctgcacc
    25801 attttccaaa acagcttcaa aaaaatcaga catttctttt gtaagtgtca ataccattgc
    25861 gtcatactct ggaagtccaa actcacggat atagcgttcg cgacgagaag ctggcatctc
    25921 aggcaaacta gcttttactt tttgaatcca ttcgtcatcg atcacgaatt tcgggatatc
    25981 aggctctggg aagtaacgat aatcactaga cccttctttg acacgcatca aaactgtttt
    26041 attcgttgtt tcgtcaaaac ggcgtgtttc ttgttggatt tctcctcctg aaagaagaac
    26101 ttttgcttga cgtttttctt caaaagctaa accttttttc acgaaagata aagagttcaa
    26161 gtttttcaat tctgctttcg taccgaactc ttcttgtcca taagggcgaa gagaaatatt
    26221 ggcatcacag cgcatagagc cttcttccat tttcacatcg ctgacttctg taaattggat
    26281 aattgatcgc aatgcttcta gataagcgta tgcttcttct ggtgaacgca tatcagcttc
    26341 agaaacgatt tcgatcaaag gcgtgccttg tcggttcaaa tcaacgtaag aataaccgcc
    26401 aataccatgc atgttttttc cagcatcttc ttctaaatgg acacgttcga tccggatttt
    26461 tttcttttta ccttctactt cgatttcgat ccatccgtca tgaccgattg gttgatcaaa
    26521 ttgggaaatt tgatatgctt ttggattatc tgggtaaaag taatttttcc ggtcaaaatg
    26581 agtatcttta gagatttcac agtttaatgc caaagctgct ttcatcccga attccagcgc
    26641 agctttattc attactggca gcacgcctgg ataaccccag tcgatcacgt ttgtattgct
    26701 gtttggttct gcaccaaaat gagctggcgc tggagagaaa atttttgagt tggtttttaa
    26761 ctcgacgtgg acttctagtc caatgactgt ttcaaaattc attcgtcttt ccctcctaaa
    26821 atagctggtt gttgtttatg gaattctgtt gcttgttcaa acgcatatgc cgcttgatac
    26881 atcgtttttt catcaaaata attaccgatg atctgcaatc caactggtaa tccttctgat
    26941 aaacctgccg gtacagacat acctggaagt ccagccaagt tgacagggat cgttaagatg
    27001 tcactcatgt acattgtgat tggatcgtta atgttctcac cgattccgaa tgcaactgtt
    27061 ggtgaagatg gcccgatgat caagtcataa tctgcaaaga ctttttcaaa atctcgtttg
    27121 atcaatgtac ggacttgtcc tgcttttttg aagtaagcgt cataataacc cgcgcttaag
    27181 gagaatgtac ctaacatgat acggcgtttt acttcttcac cgaatccttc tgaacgtgag
    27241 ttgacatata catcatctaa tgatttcaca ttttcagagc gataaccgta gcggatacca
    27301 tcgaatcgtt gcaaattgga acttgcttct gatgaggcaa tgatataata aacagccaca
    27361 ccgtattttg aatgaggcaa gctgacttct tccacaacag cacctaatga acggaatgtt
    27421 tctgctgctt tcaatacagc ttcttttacg ccaggggcca caccttcgcc taagtattct
    27481 ttaggtaaag caatcttcat tcctttgatg tcgcctgtca gtccctctgc aaaatcagga
    27541 actgaaacgc ctgaagaagt accgtctttt tcgtcataac cgctgatagc tgtcaatgct
    27601 aatgcattat ctttcaccgt acgggtcatt ggtccgattt ggtcaagaga tgaagcaaag
    27661 gcaatcaaac caaaacgtga gacacggcca taagtaggtt tcatccctac gatgccgtta
    27721 aaagctgctg gttggcggat acttccccca gtatcactac ctaaagaaac tggaatttgt
    27781 ccagctgcaa ctgctgctgc agaaccacca gaagagccac caggtacttt tgttgggtcc
    27841 caagcatttt ttgttttctt gaaataagaa gtttctgtac ttccacccat agcaaattca
    27901 tccatgttta gcttaccaac agggatcata tctgcttgat agattttatc catgaccgtt
    27961 gcatcataaa ttggttcaaa attgtaaagg atcttagatg cagcggttgt caagatatct
    28021 tttgttacga tattatcctt gataccaatt ggaataccag caaggacgtt gctttcatca
    28081 atcccttttt catctaaagc ttttgcctgt tccaatgctt tttcttcgtt caatgtaatg
    28141 aatgcctcga cctcaggctc tgtttctttg attcgttcaa atgttgtttt tgtcaaatct
    28201 tgtacagtga tttctttcga tacaagcata tgatgcagtt cttctagaga acgattgtgt
    28261 aattctgtca ttatgcgcca gcctccccat tatcgatgat tgcgggtact ttgataaagc
    28321 cgtcttctgt ttccggcaca tttttcaata attcatcgcg gttccatcca gcttgcgccc
    28381 gatcttgtcg catcacgttt actgtttcaa ccacattcga cgtaaatgga acaccttccg
    28441 tatctacttc ttccagcatt tcaaccatat cgatgatttt acctaattgg tcggtaaaac
    28501 ttgcaagttc ttcatctgcg aacgcaagtt ttgctaattt ggcaacgtgt ttcacttgtt
    28561 cttcactaat cgccatacta ccccttcttt ctaaacgagt taaaaacttt gctctctatt
    28621 ttaacacaag agtgccgtgt ctgaaattgt ttattgaaat ttgtctgaaa aaactcattt
    28681 ttcattcttc tactgagcga cttttagcca atgcatctgc catttgatct tctgtccaaa
    28741 cttcgattcc taattcttgt gctttggtca gcttgctgcc agcatcttct cctgctacca
    28801 cgatatccgt ttttttcgat acgctaccag tcactttccc gccaagattt tcgatcgtct
    28861 cttttgcttc ttcacgagta aatcgtgtta gtttaccagt aaggactact gttttttctt
    28921 taaaaggaga ctcgacttct tgcaattggg catttcgaag ccctttgtat tcgaaattga
    28981 cgcctgcttg ttttaattca ttcatcaatt catggacttc ttcgttactg aaataagtga
    29041 ctacactgtc ggcaatgata tcaccgatcg tatcaagcgc aataatcgac tcatagtcac
    29101 ttttactcaa tgtttctaga tcaccgaaat gttctgctaa aatttttgct gctttcgctc
    29161 cgacatgtcg gattcctaac ccgaaaatca accgctcgac cgaattatct ttgctgttgt
    29221 cgattgctgt caaaatatta tttgctgatt tttcttttat tttgtccaac gtcaataact
    29281 cttcttctgt caatttgtaa agatcagcaa catctgctac tagtttttta tcatacatct
    29341 gttctagtac gcgcggcccc aacccatcga tattcatcgc atttcttgaa acaaagtggt
    29401 tcaaaccttc tttcatttga gcagggcatt taggattgat gcaccgtaag gctacttctt
    29461 catccaaatg gacaagttca cttccacaaa ctggacaatg tgtaggcaat tggtattctt
    29521 cgctgtcttt aggtcgttta tctaacacga cttgcgaaac ttctgggatg atgtctcccg
    29581 ctttataaat cagaactgta tctttcaagc ggatatcctt caacttaata taatcaccgt
    29641 tatgcaggct ggcacggctt actgtcgttc ctgctactcg gactggtgtc atgatagctg
    29701 ttggcgttac tacccctgtt cgaccgactg tccattcgat gttttcgatt agcgtttcaa
    29761 cttcttctgg cggaaacttg taagctgctg cccatctcgg tgctttgata gtaaaaccaa
    29821 gttgatcttg tagcgagaat tcattcactt tgatgacgat tccatcgatt tcatagggca
    29881 aatccacacg tttgtcatga tattcttcaa tgtatgacca cacttcatca atcgaatgac
    29941 atagacgttt ttcccgattc gtgtggaatc caattttttc tagctcttct agcgcatcat
    30001 actgggtttt tgcttgcata ggaccgaaat cagctactgt gtataaaaaa gtgtccagat
    30061 ttcttttcgc tgtgatttta gaatctaact gacgcaaaga accagctgcg gcgttccgag
    30121 gattcgcaaa gatatctttt ccctctgctt ctctgtcttg gttcaattgg acaaatgacc
    30181 gttttggcat gaaacattcg cctcttactt cgatgttcat tggttctttt aatttgattg
    30241 ggaccgaccg gacagttttt aggttttcag tgatgttttc tcctacagtt ccatccccgc
    30301 gagtcgcacc tctcacgaaa tttccatctt cataacgcaa agaaacggaa agtccatcta
    30361 tttttagttc acagcaatag tcaactacac gtcccactgc tttttgtaca cgttgatcaa
    30421 aagctatcag ctcttctttg ctgaaaacgt cgttcaaact gtaaagcgga atatcatgag
    30481 tgactttttc aaaaccttct aatacttttc cgcctactcg ttgagtggga gaatcagacg
    30541 tgatcaaatc aggatattgt tcctcgattg ctactaattc tgcgtattct ttatcataaa
    30601 catagtcttc cactgttggt ttatcttcta cgtagtattc gcgagaccat tggttcagtc
    30661 ttgtacgaag ttcttctgct cgtttagcag cttcgtcaat cgtcatttca tgttccatca
    30721 tttaccctcc actactaagc tttttcaatt ggcgcaaagg cagccaaaag acgttttact
    30781 ccttgacttg ggaaagccac atctagttct agatccttcg acgtcccgct tactcggacg
    30841 actgttccta gaccccattt tttgtgtttg actttatctc caggtttcca gctgttattt
    30901 tcccctccag tagcactttt agagggaaca accgctgtct ctggctgttt atagctatat
    30961 gctgtttttt tagcgaatac aggcgttttt ttaggcgtag gacgcatgcc ttcgatttcc
    31021 agtaactcct gatcgatttc ttctacaaaa cgagaaggtc ggttatattg cgtccgtcca
    31081 tataatgttc tggagaaagc atttgttaaa tacaacgctt cttctgctcg cgtgatcccc
    31141 acatatgcga gacggcgttc ttcctccagt tcgctttctt ccatcaatgc tcgagataaa
    31201 ggaaaaaccc cctcttctag accaatcagg aatacaactg gaaattctaa tcctttggct
    31261 gcatgcaaag tcatcaatgt cacttgggaa gcatcttctt ctaaattgtc aatgtcggaa
    31321 acaagtgcca gatcatttaa gaagaccgtt agtttttctt ctggtgcatc agcgtcttct
    31381 tcattttgct gttcgaattg tttgtcgaat tcttgtgtga ccgttaaaaa ttcctccaag
    31441 ttttcgatgc gtgcttgtgc ttctaatgta ttttggattt ttaagtcttc tagatatccg
    31501 ctacgatcca aaacctcttt cgtcaattct gtcactgtca agtatgggat catctgagtg
    31561 acttcttgga tcatctctcc aaatgcacct aattgctgtc ctgcttttcc accgatattt
    31621 gccagatcca cattttgcgc tgcttcaagc agtggccatt catgaagaga agcaaaactg
    31681 cgtagttttt cgatagatcc tggtccgatg cctcgtttgg gcgaattaac gatacgctca
    31741 aaactgattg aatcttgagg atttaccagc acattcaaat acgctaaaat atctttgatt
    31801 tctttacgat cgtagaactt gtgccctcca accattttat aaggaatatt tgctttcaac
    31861 agagtttctt ccattacacg agattgtgcg ttcgtccggt acaagatggc aaagtcgccg
    31921 taattccgat gattgcttcg aatctcttct tgcatccggt ctacgataaa acgggtttcg
    31981 tctcgttcat tatctgcacg gtagtaagtg atcttgtttc cttctttgtt ttctgtccac
    32041 aagttttttg gttttcgatt gctattgttt tcaatgactt ggtttgctgc gctaaggatg
    32101 tttttcgtgg agcgatagtt ttgttctagc aaaattactg cagcatctgg ataatctttt
    32161 tcaaaatcca agatgttctg catatctgca ccgcgccagc cgtaaatact ttgatctgca
    32221 tctcccacga cgcacaggtt cctaaatcta cctgccaata aattgactag cgtatactgt
    32281 gcgtgatttg tatcttggta ctcatctaca tgaatataat ggaatttgtt ttgataatac
    32341 gtgagtgaat caggatgttc ttcaaataat cgaatcgtat tcatgatcag gtcgtcaaag
    32401 tccatacatt gattatattg aagctctttt tggtaagcag cataacattt tgccgcaatc
    32461 tcttcgtaat aactgccttg catttttgca aaatcctgag gggtctgcag gctgtttttt
    32521 gcttgagaga ttgttcctaa tatacttcga ggatcatatt ttttcggatc aatgttcagt
    32581 tcttttagta tccgtttcat caaggtcagc tgttcagaag aatcgatgat tgtaaagttc
    32641 cggttatacc cgataaaatc aacatctctt cttaaaatac gcacacacat cgagtgaaaa
    32701 gtcgataccc agacatcttc gcctccagat gcgagaatcg cattgacacg ttctttcatt
    32761 tcttttgcag ccttattggt aaaagtaatc gctaggatgt tccaaggatt gacttctttt
    32821 tcctcaatca aataggcaat gcggtgtgtc agcactctcg tctttccact tcctgcacct
    32881 gccattagca gtaatggtcc gtcagtgtga agaacagctt ctttctgacg ggggttcatc
    32941 ccgtttaata attcagcttt ggatgacatt aacaacatcc tttcttttat caatcttgtt
    33001 aattatagca ttttcttggc tttcattaaa ctagctagtt caaaatcatt tgtttctttt
    33061 tatggatttt tccttgtcaa ttcgttgatt tcgcgctaaa ttaaaagaaa gcgaggcgtt
    33121 tgaatggaaa aaaaaggaaa aatcaagaaa ttagataaga ctcagatata ttttattcta
    33181 aaaggaatat ttgttggact gctgacagga atcgtcgtta gcctgtttcg attatctatc
    33241 gaaaagctaa gcgatttagt tcgaagtatc tatgaaatga gtcgggaaca gccgatttac
    33301 ttaattggcc tagctatttg ctgcatcctt gcagcatttt tcgtgggtta tctggtcaaa
    33361 aatgaaccgg atatcaaggg aagcgggatt ccacaagtag agggacagct tcgcggggaa
    33421 ttatcaatga actggttctc tgttttatgg aaaaagttca ttggcggtgt actttcagtt
    33481 ggtgcgggcc tttttctagg tagagaagga ccttctatac agcttggcgc atccattgga
    33541 caaggagctg gacaattgtt tcggagtcca tcttcagaag aaaagatttt aatttctagc
    33601 ggtgcaagta gcggacttgc agcagcattc aatgctccta tcgcaggatt actctttgtt
    33661 ttagaagaaa tccatcactc cttttctcca cttgtttggc tgacatcatt tgcttcagca
    33721 atcactgcta acttcgtctc tctttacttt ttcggcttga gacctgtttt atatctaggt
    33781 gaccttcctt ctttgccgtt gaaatattat ggttccttag tgatcttagg agtacttcta
    33841 ggcatcttag gttttattta tcagaatgtc ttgctggctt tgccaaaatt ttacaaccga
    33901 ttgcctttac cagcttattt tcacgggttc gttcctttct tgctgatttt gccaattggt
    33961 tatttatggc ctagtactct aggcggaggc aatcaaatcg ttttagcttt tggacatgct
    34021 tccttgccaa tcattatgat tatcggtttg tttgttcttc gattcgtttt ttccatgatt
    34081 tcttacggtg ggggtcttcc aggggggatt ttccttccga tattgacatt gggggcttta
    34141 atcggtagct tttatggaaa cattatggtt gatttcggga tggatccaat ctacatcaag
    34201 gattttgtcg ttattgccat ggctggttat ttcacagcga ttggaaaagc accgctgaca
    34261 gcaattattt tagtgacaga aatggttggc tctcttaatc atctgatgcc tttaggattg
    34321 gctgctcttg ttgcttatat cgtcaatgac cttctcggcg gtaatccaat ttacgaatca
    34381 ttattggaac gtctacttgg aaatactcag accgcctcac taaaaggtgc aaggacaact
    34441 tttgattttc cagtaacggc agagagttct ttagacggat taatggtacg agacttcaat
    34501 tggcctaaaa atatgctgct aatctctata cgcagaggaa atcaggaaat cttgactcac
    34561 ggtgatacga tcatgaatgt aggagatatt ttggttatct tgactgatga agcaaacttg
    34621 ccaagaatca agcaggaaat cgaggctaaa tcttctatca caaatttgaa gcaatcattt
    34681 taacgaacat ggaaaaacta ttttacaaca actattcatc tacactactt gattctatat
    34741 gattctataa aaaaagaaga ggctgggaaa a
//
LOCUS       DANKMN010000024        34650 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000024 DANKMN010000000
VERSION     DANKMN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            598..1287
                     /locus_tag="PW224_001116"
     CDS             598..1287
                     /locus_tag="PW224_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2907822.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="PW224_001117"
     CDS             1274..2482
                     /locus_tag="PW224_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2907823.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="PW224_001118"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="PW224_001118"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL2907824.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="PW224_001119"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="PW224_001119"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL2907825.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="PW224_001120"
     CDS             complement(6585..7280)
                     /locus_tag="PW224_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL2907826.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="PW224_001121"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="PW224_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL2907827.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="PW224_001122"
     CDS             complement(8479..8670)
                     /locus_tag="PW224_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL2907828.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="PW224_001123"
     CDS             complement(8691..9581)
                     /locus_tag="PW224_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL2907829.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="PW224_001124"
     CDS             complement(9568..10335)
                     /locus_tag="PW224_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2907830.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="PW224_001125"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="PW224_001125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL2907831.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="PW224_001126"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="PW224_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL2907832.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="PW224_001127"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="PW224_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL2907833.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14906..15289
                     /locus_tag="PW224_001128"
     CDS             14906..15289
                     /locus_tag="PW224_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907834.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="PW224_001129"
     CDS             complement(15457..16215)
                     /locus_tag="PW224_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL2907835.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="PW224_001130"
     CDS             complement(16233..17051)
                     /locus_tag="PW224_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2907836.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="PW224_001131"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="PW224_001131"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL2907837.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="PW224_001132"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="PW224_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL2907838.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18180..19514
                     /gene="dnaA"
                     /locus_tag="PW224_001133"
     CDS             18180..19514
                     /gene="dnaA"
                     /locus_tag="PW224_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL2907839.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19712..20842
                     /gene="dnaN"
                     /locus_tag="PW224_001134"
     CDS             19712..20842
                     /gene="dnaN"
                     /locus_tag="PW224_001134"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL2907840.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21071..21313
                     /gene="yaaA"
                     /locus_tag="PW224_001135"
     CDS             21071..21313
                     /gene="yaaA"
                     /locus_tag="PW224_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL2907841.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21300..22424
                     /gene="recF"
                     /locus_tag="PW224_001136"
     CDS             21300..22424
                     /gene="recF"
                     /locus_tag="PW224_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL2907842.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22421..24367
                     /gene="gyrB"
                     /locus_tag="PW224_001137"
     CDS             22421..24367
                     /gene="gyrB"
                     /locus_tag="PW224_001137"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL2907843.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24387..26858
                     /gene="gyrA"
                     /locus_tag="PW224_001138"
     CDS             24387..26858
                     /gene="gyrA"
                     /locus_tag="PW224_001138"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL2907844.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27094..27393
                     /locus_tag="PW224_001139"
     CDS             27094..27393
                     /locus_tag="PW224_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL2907845.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27443..27955
                     /gene="ssb"
                     /locus_tag="PW224_001140"
     CDS             27443..27955
                     /gene="ssb"
                     /locus_tag="PW224_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2907846.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27981..28217
                     /locus_tag="PW224_001141"
     CDS             27981..28217
                     /locus_tag="PW224_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL2907847.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28360..30333
                     /locus_tag="PW224_001142"
     CDS             28360..30333
                     /locus_tag="PW224_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL2907848.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30339..30791
                     /gene="rplI"
                     /locus_tag="PW224_001143"
     CDS             30339..30791
                     /gene="rplI"
                     /locus_tag="PW224_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL2907849.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31065..32432
                     /gene="dnaB"
                     /locus_tag="PW224_001144"
     CDS             31065..32432
                     /gene="dnaB"
                     /locus_tag="PW224_001144"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL2907850.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33212..34504
                     /locus_tag="PW224_001145"
     CDS             33212..34504
                     /locus_tag="PW224_001145"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL2907851.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10551 a         6728 c         6852 g        10519 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgcggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggag tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaatgg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg tgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tatatttttt atccacagat gcaagaggaa atctattgat ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa cttttttttg acaactgtgt ttttctcttt tttctttttg
    18061 tcgattgtgt ggataattgt aattaaaaaa tttttttctt cggtttctgt ggaaaactat
    18121 tgaaaaaaat gctaaattag tactagttat tcacttttta atgaaggagg aggccttata
    18181 tggtatccct cgatgcttta tggaatgaat taaaagcaac ataccaaaag gatttatcgc
    18241 ctgctagcta taatacatgg atcgaaacag cgaaccctcg aacccttgat caaaaccagc
    18301 tcgttgttga ggttcctagc aagattcata aagaatattg ggaaaaaaac ctggcgacaa
    18361 aaatcgttga agtcggttat atgttatcag gaaatgagat tatccctcgc tttattactg
    18421 gcgaagaagc agagcaagaa gaagttatag aagaaaaaaa tccaaaagtt gtggcaccta
    18481 gtccgctaaa aaaagccatg ctgaacccta aatatacctt tgatacgttt gtcattggta
    18541 aaggaaacca gatggctcat gcagcagcgc tcgttgtagc agaagatcca ggttctattt
    18601 ataacccgct cttcttttat ggaggcgtgg gacttggtaa gactcacttg atgcatgcaa
    18661 ttggtcatca aatgctgcaa agtcagccta atgccaaagt aaaatatgta agcagtgaaa
    18721 cttttgctaa tgatttcatc aattcgatcc aaaacaaaac agcagaagag ttccgtcaag
    18781 aatatcgaaa tgttgattta ctgctagtgg atgacattca attttttgca gaaaaagaag
    18841 ctacgcaaga agagtttttc catactttca atgccttgta caatgaaggg aaacaaatcg
    18901 tactaaccag tgatcggttg ccaaacgaga ttccgaaact gcaagaacgc ctcgtttctc
    18961 gctttgcatg ggggctgtct gttgatatta caccgccgga tcttgaaaca agaacagcga
    19021 ttttacgtaa aaaagcggcg gctgaacgtc tagagatccc agatgacaca ctaagctaca
    19081 tcgcaggaca aattgattct aacattcgtg aattagaagg tgcgcttgtt cgagtgcaag
    19141 cttttgctgc gatgaatagt gaagatattt cgactagtct agctgcagac gcactgaaaa
    19201 cgttaaaatc aggaaaagga catcctcaat tgtcaatcct gcagatccaa gaagaagtgg
    19261 caaagtatta tcacatccaa ttgaaagata tcaaaggaaa gaaacgcgtc aaatctattg
    19321 ttgttcctag acaaatcgct atgtatttgg cacgtgagct cacagataat tctttaccga
    19381 aaatcggagc tgaattcggt ggcaaggatc atacaacggt tatccatgct catgaaaaaa
    19441 tccagcagct gatggattct agtgtcagta tgcaaaatga agtatcagaa atcaaaaact
    19501 ttttactaaa ttaatttgtc gattctatca actgaaaata gcatgtggat aaaagaaaaa
    19561 aacgttcaaa agttatgcac actttttcca cagctgtttt ttcgtttctc tcttacgttt
    19621 tttgagtttt ccacagaatc aacatgccct attactttta ttattctttt taactaataa
    19681 taaataaaca aacattcaaa ggagatacat catgaaagtt actttaaacc gagctagctt
    19741 tatgcaggaa ttgcaaactg ttcaacgagc tatttcaagc aaaaccacga tccctatttt
    19801 gacaggtgta aaaatcacac tgacacaaga aggtttgact ttgacgggga gcaacgctga
    19861 tatatcaatt gaaacttttt tgtctgttga aaacgaaaaa gcaaatatgc aaatcgaatc
    19921 tactggttcc attgttttac aagcacgttt ctttagcgaa atcattcgga gacttcctga
    19981 agaaacattt actttagaag ttttagaaaa taaacaagta gcgattactt ctggaaaagc
    20041 gaattttatc gtaaatggat tagatgcaga taactatcct catcttcctg ttgtcgaaag
    20101 ccataaccag atgaaattac ctgtacacgt attgactaaa ctaatcaacg aaacagtttt
    20161 tgctgtctca caacatgaga gtcgtccaat cttgacaggt gtgcatttca ttttatctga
    20221 taattcttta ttggctgtag ctactgattc tcaccgtcta agtcaacgcg tgattccagt
    20281 agaacaagcg gctgatcatt ttgatattgt tattcctgga aaaagtttga tcgaattatc
    20341 tcgctcatta acaaatgaag aagaaatcgt cgaaatcagc attatggaaa accaagtgtt
    20401 gttcaaaaca gaaacgatgt atttctattc ccgtttgcta gaaggaaact atcctgatac
    20461 caatcgtttg attccttcaa gttttaatac agaggttgaa ttttctgttc ctagcttttt
    20521 agcggcgatc gaacgggctt ctttactttc tcatgaaggc cgtaacaaca tcgttcgttt
    20581 atctattcgt ccagatgcgg ttgttttata cggaaattcg ccagaaatcg ggaaagtaga
    20641 agaaagcttg agttatacag caagcagtgg cgatccatta gatatttctt tcaatccgga
    20701 ttatatgaaa gcagcgttgc gtgcatttgg agatatgagc atcaaagtga aatttatctc
    20761 tgctattcgt ccatttacat tggaaccaac ggaagatggc gttcaattta ttcagctgat
    20821 tactcctgta cgtacaaatt aacaatgatt ttcattgttc ttgaaaattt aattgaaaag
    20881 aaatagaaaa aataaaattt taaacagaag gccctaaaat ctcttttaag gctttctgtt
    20941 tatttttgtt taaatcacct tttttagatt aaatcgtctt atatggaaaa atatatggat
    21001 aaatttgttt tattgcgaaa aaaaaggtat aatagagtat acttttaata atgaaaatga
    21061 gggatgggca ttgaaacaac aagtcattct aaatacggaa ttcatgacgt tgggccaaat
    21121 gttgaaagaa gtcacagtta tcggtagtgg cggacaagca aaatggtatt tggcagaaaa
    21181 cactgtgtta gtcgatggcg aaccagaaaa tcgtcgtgga cgcaaacttt atcccggcat
    21241 gatggttgaa gttccagaag taggaacttt ttttatggta aaaaaaggaa acgacgataa
    21301 tgaggctgaa taagctgtat ttaaaaaatt atcgaaatta cgaagagctg aacatcgaat
    21361 tttccaagaa cctcgtcatc tttttaggag aaaatgcgca aggaaaaacg aatcttttgg
    21421 aaagtatcta cgtattagct atgacgcgaa gtcatcggac aagtagtgag caggaattga
    21481 ttggctggac tgacgatcag gcgatgattc aaggagagat cacaaaaggt tcatccactc
    21541 ttccattaga aatcttatta tctaaaaaag gccgaaaaac caagatcaac catatcgaac
    21601 aaaaaaagtt aagtacttac gtgggacaaa tgaatgtcat tttatttgca ccagaagatc
    21661 tttctttagt aaaaggcagc ccgcagatca ggcggaagtt tttagatatg gaattaggcc
    21721 agatcaatcc aatctatctt tatgatcttg tccaatacca agcaattttg aaacaaagaa
    21781 accagtattt aaagcaattg aatgaaaaaa aacaaactga tttgctttat ttggatattt
    21841 tgtccgaaca actagcagcg tttggcagca aagtattgaa agcaagagca cagttcgtca
    21901 agcgattgga gttttgggct aattctttgc atcagcaaat cacccatcaa aaagagcagc
    21961 tggaaatcga gtatctcacc gcagtcgatt ctttagaaac acatacgcaa gagcaaatcc
    22021 aagaacaatt tctcgcactt ttgaaccaaa ataaaaagaa agaccttttt cggggaacaa
    22081 cgacagttgg tcctcatcgt gacgatcttt ccttttttat taatcagaaa aatgtgcaga
    22141 cttatggatc gcaaggacag caaagaacaa ctgcactaag tgtcaaatta gcagaaatcg
    22201 atttgattaa agaagagacg ggggaatatc caatcctttt gttagatgat gtgatgagcg
    22261 agctggatga tagtcgtcaa ctccatctgc tagaaacaat cgaaggaaaa gttcaaacgt
    22321 tcttaacaac aacgacactt gaccatgtaa aaaataaaat gactgttgaa cctgagattt
    22381 tttatgttca acaagggaaa ctaaaaggag aagaaactga atgacagaag aaagaagttt
    22441 agtagaacgc gctaaagagt atgatgccag tcagattcaa gtacttgaag gacttgaagc
    22501 cgttcgtaaa cgacctggca tgtatatcgg ttcgactagt tctgagggtc tgcatcacct
    22561 tgtttgggaa attgtcgata attcgatcga tgaagtatta gcaggttttg caacgaaaat
    22621 ccatgtgatt attgaaaaag acaacagtat caccgttatt gacgacggtc gggggatacc
    22681 tgtagatatc caggcaaaaa ctggacgtcc tgcagttgaa acggtcttta ctgttttaca
    22741 tgctggagga aaattcggcg gtggtgggta caaagtttct ggtggacttc acggagtagg
    22801 atcttctgtt gttaacgcgc tttctacaca gcttgatgtc aaagtataca aagaaggaaa
    22861 tgtgtactat caagaatatc gtcgtggagc tgtagtggac gacttaaaga ttatcgaaca
    22921 aacggatcgc catggaacaa cggttcactt tacgccagac cctgaaattt ttacagagac
    22981 aactgagttt gatttttcaa aattggctac ccggatccgt gaacttgctt tcttgaaccg
    23041 cgggatgcgt atatcgattg aagacaaacg agaagaagaa ccagtcatca atgaatacca
    23101 ttatgacggc gggattaaaa gttatgtcga atacttgaat gcaaacaaaa ccgtgatttt
    23161 tccagaacct gtatatttgg aaggtgaaca gcaagatatc gcagttgaag tctctatgca
    23221 atatacagac ggctaccatt ccaatatcat gagttttgcc aacaatatcc atacgtatga
    23281 aggcggaaca catgaatcag gattcaagac tgctttaact cgtgtgataa atgattatgc
    23341 ccgaaaacaa aagctgatga aagaaaatga cgacaacttg accggtgaag acgtgcgaga
    23401 aggattgaca gctgttattt ctatcaagca tcctgaccct caattcgaag gacaaacaaa
    23461 aacaaaattg ggcaattccg aagtacggac agtcactgat cggttattta gcgaacattt
    23521 catgaaattc ttgctggaaa acccaagtgt gggccgtcaa attgttgaaa aaggattact
    23581 agcttccaga gctcgtctag cagcgaaacg tgcgcgtgaa gtaacgagac gtaaaggtgc
    23641 tttagagatt agtaatctgc caggaaaatt agcggactgc tctagcaacg atcctgaaaa
    23701 atgcgaattg ttcatcgtcg aaggagattc ggccggcgga tcagctaagc aaggtcgtaa
    23761 tcgagagttt caagccatct tgcctattcg tgggaaaatc ttgaatgttg aaaaagcaag
    23821 catggacaag atcttggcaa atgaagagat ccggtcttta tttactgcta tgggtacagg
    23881 gtttggagaa gattttgatg tatcgaaagc tcgctaccac aaattagtta tcatgactga
    23941 tgccgatgtc gacggcgcgc atatccgtac attgctgctg actctttttt atcgttacat
    24001 gcgtcctatt gttgaagctg gatacgtcta tattgctcag ccacctttat acggggtaaa
    24061 acaagggaag aatattacgt atgtacaacc aggaaaaaat gcagaagaag agttgaaaca
    24121 agtcgttgct tcacttcctg cctcaccaaa accaagcgtt cagcgttata aaggacttgg
    24181 agaaatggat gatcatcaac tatgggaaac aactatggac ccaagcaatc ggatgatggc
    24241 ccgcgtcagt gtggacgatg ctattgcagc agatcagata tttgaaatgt tgatggggga
    24301 tcgagtagaa ccccgtcgtg cattcatcga ggaaaatgca cactatgtga agaacttaga
    24361 tatttaatca tgaaggagga aaaagaatga gtgaagaaat cagagaaaac atccatgatg
    24421 tcaatctaac cagcgaaatg aaagactcct tcatcgatta tgcgatgagt gttattgtcg
    24481 cacgtgcact tcctgatgtc agagatggtc tgaaacccgt tcatcgccgt attttatacg
    24541 gcatgaacga attaggtgta acaccggata aagcccataa aaaatctgcc agaatcgtcg
    24601 gagatgtcat gggtaaatac catcctcatg gagactatgc gatctatgag tcaatggtac
    24661 ggatggcaca gccattcagc taccgttata tgctagtgga tggtcatgga aactttggtt
    24721 ctgttgacgg tgacggtgcc gccgctatgc gttataccga agcacggatg agcaaaatcg
    24781 ctacagaaat gctgcgtgat atcaataaga acactgttga tttccaaagc aactatgatg
    24841 atacagaaaa agaaccagtt gtactacctg ctcgcttccc gaatctttta gtgaacggaa
    24901 caacggggat cgccgtaggt atggccacga atatcccacc tcataacttg tctgaggtag
    24961 tagcggctat cgatttgctg atggataatc ctgatgtgac gaccaatgaa ttgatggaag
    25021 tattacctgg accagatttt ccaacaggtg gtttggttat gggaaaatca ggaatccgaa
    25081 gagcgtatga aacaggtaag ggttctatta ccgtacgtgc aaaagttgag attactgaga
    25141 tgccaaatgg taaagaacgg atcatcgtga cagaattgcc ttatatggtc aataaagcaa
    25201 aattgatcga acggatttct gaactgcaca gagacaagcg tatcgaaggg atcacagatt
    25261 tgcgggacga atcttcccgt gaaggtatgc ggatcgtcat tgacatccgt cgtgacgcca
    25321 gcgcatctgt tatcttgaat aacttatata aattgacatc cttgcaaaat tcatttggat
    25381 tcaatatgtt ggcgatcgaa aaaggtgtgc cgaaagtttt gagcttgaaa caaattcttg
    25441 aaaactatat cgaacatcag cgcgaagtca tcacaagacg gacagtcttt gaaaaagaaa
    25501 aagcagaagc aagggcacac attttagaag gtttgcgtat tgctttggat catatcgatg
    25561 aaatcattgc gattatccgc gggtctaaat cagatgacga agcaaaagcg acgatgatcg
    25621 aacggttcga tttatccgat cgccagtctc aagctatttt agatatgcgt ttacgccgat
    25681 tgaccggttt ggaacgggaa aaaatcgaaa atgaatacca agaactctta aaactgattg
    25741 aagatttaac tgacattctg gcaagaccag aacgtgttac agaaatcatc aagaatgaat
    25801 tagcagactt gaatcaacga tttggtgata aacgacgcac agaattacta gtcggtgagg
    25861 tcttaagtct ggaagatgaa gatttaatcg aagaagaaga aatcgttatt acattgacaa
    25921 acaatggtta catcaaacgt ttggcaaata atgaattcag agctcaacga aggggtggac
    25981 gtggtgtcca aggaatgggt atccacaatg acgacttcgt caaaaatctg gtttcttgtt
    26041 ctacccatga tacattgtta ttctttacga ataacggaaa agtgtatcgg gcaaaaggat
    26101 acgaaattcc agaatacggg cgcacagcca aaggaatacc aattatcaat ttactaggta
    26161 tcgattcgag tgaaagcatc caggcaatca ttgcggtgac gggagaagca gaagaaggcc
    26221 actatctctt ctttacgacg cgtcaaggta cagtaaaacg tacttctgtc actgcttttg
    26281 ctaacatccg aagtaatggt ctgatcgcaa ttggtttgaa agaagacgat gagctcgtca
    26341 atgtgctact gacagatggt caatctaata tcattattgg aacacataac ggctactctg
    26401 tcactttcaa agaagaagca gtccgcgata tgggacgtac agcttccggg gttagaggaa
    26461 tacgcctgcg tgaggaagat tatgtcgttg gggcagcctt aatgaaggaa gatcaagaag
    26521 tattagtgat cacagaaaaa ggttacggaa aacggacaaa agtatcggaa tatccagtga
    26581 aaggccgcgg aggtaaaggg atcaaaacgg cgaacatcac tgaaaaaaat ggtccactag
    26641 ctggattgac cactgtttct ggagatgaag acatcatgct gattacagat aaaggtgtca
    26701 ttatccgttt caacgtatcg actgtttctc aaactggacg ttcaacatta ggcgtacgtt
    26761 tgatgaagat ggaagctgat acgaaagttg tcacgatggc agcagtcgaa ccagaagctg
    26821 cagaacaaac agttgaagaa caaggatcag aagaatagcc atcaagacta attgtgtaag
    26881 ctagcaagaa tgaccgatta aaaaatctgg ttattcctgc tggctttttt gattagttga
    26941 atttctctta aaatattcga aactgttcat tctgtattgc ttttgaccaa aatagagtgt
    27001 ataatcagag cttgtgagta gtctaattaa ttagataact ctccttgctc ttaggaacct
    27061 aagagccgaa agtccacagg gaggtgaata tcaatggaaa acacgaaata tgaaatcatg
    27121 tacattattc gtccaaacat tgatgaagaa gcaaaaactg ctttaatcga acgtttcgat
    27181 gcaatcttga aagataacgg agcagaagtt atcgaatcaa aagattggga aaaacgccgt
    27241 ttcgcttatg aaatgaacgg ttaccgtgaa ggtatctatc acatcgttaa agtatcttct
    27301 ccatcatcag caaatgctgt caatgaattt gaccgtcttg ctaaaatcaa cgacgatatc
    27361 attcgtcaca tgattgttaa agaagaagct taattgttgt ttcatgtgaa acaatagaaa
    27421 acgatgagag gagatgacta ttttgattaa taatgttgta ttagttggac gtctgacgaa
    27481 agatccagat ttgcgttaca cagcaagtgg tacggcagtc gcaacattta ctttggctgt
    27541 gaatcgtaat tttacaaacc aaagcggcaa tcgcgaggct gattttatca actgtgtgat
    27601 ttggcgcaaa tcagcagaaa ctttagcaaa ctatgctcgt aaaggaacac ttttaggtgt
    27661 tactggacgt atccaaactc gttcttatga taatcagcaa ggacaacgtg tatacgtgac
    27721 agaagtagta gctgacaact tccaattgct ggaaagtcgt tctgcatctg aaaatcgcca
    27781 acaaagcggc ggattccaaa gttctggtca atcagctggc ggatttggcg gaaacaataa
    27841 ctctaatcaa acatctcaat catccaacgg tatgcctgat ttcgaccgtg atacttctga
    27901 cccattcggc tcaagttcaa cgatcgacat ttcagacgat gatttaccat tctaaacgaa
    27961 tttgatagga gggaaataga atggcacaac aaagaagagg cggacgtaaa cgtcgtaaag
    28021 tagactacat tgctgctaac catattgaat atatcgatta taaagatatc gaattactaa
    28081 aaagatttat ttcagaacgc ggtaaaatct tgccacgtcg tgtaactggt acaggcgcta
    28141 aaaaccaacg taaaattact gttgcaatca aacgcgctcg tatcatgggc ttgctacctt
    28201 tcgttagcga agactaataa atcctaggaa ttgaatgact gatccttttg atcagctatt
    28261 caattccttt ttttctgtaa aaataacctc tattcgttta gtcgctcgaa tgacgaaagt
    28321 tatggtaaaa tcaaattagt catatatggg aggaaaatta tggaaaataa aaacaatcat
    28381 ttatccccga aacctctctt ccttatcctg tttatattaa tcgaagtatt ggtagtctta
    28441 attttaccat ttcgttttgt tagcgcttcg gtcattctat tgatgaattt atggggtgtt
    28501 cgctattggt tgaaaatgaa acgttggctt attttaaata aacgtgaaaa aatccagcag
    28561 gcttcgactg tagctgaaac caatctagct tatgtctcag aagtcatgcc tgtaggagtg
    28621 attgcttatc agccatcaag ccaaaaaatc gaatggctca atccattagc attgaaaatc
    28681 aaagatcaaa gtggattaac agaggaagaa ttgctagata ctttttttac tgcaaaagaa
    28741 aaaaagaaaa accaagtcta tctagcagat aaaacatttc agtttgatat ggatacagta
    28801 aagggtgttg tttattttaa tgatattacc caaaaccagt tgttaaaact ccatcagacg
    28861 aacagtcagc cggtgattgg tattttatct attgataact atgcagatgc cattgataaa
    28921 ttggatgaca aagagatttc ttatttgaac tctttcgtca ctacactagt ttctgactgg
    28981 atgaatgaat accatgtctt tttcaaaaga atcaacgcag aacggttttt cttcgtagca
    29041 cagatgatcg atctgaaaaa gatgatggat aaaaaatttg atttgctgga tcgctttcga
    29101 gcagaagctg aaaaacagag ctcgcctatc acccttagta tggggattgc ttatggctca
    29161 agaaccctcg atgaaatcgg tggagaagct cagaataatt tagatgttgc tttagtccgt
    29221 ggaggcgacc aagttgtcgt gaaagaggta aaagaaggag caaaacctgt ttattatggg
    29281 gggaaatctg ctaagaatgt caaacgcacg cgtgttcgtt ctcgagcgat gagtaccgct
    29341 ttgcgtcgga tttttgatga aacaggcgat gtgtttatca tggggcacaa gtttccggat
    29401 atggatgctt taggctctgc ttttggtgtc agttgtctgg cacgcttcaa ccagaaaaaa
    29461 gcttacatcg ttatcaacga gaacgagatc attccagatg tagaacggtg tcttgaagaa
    29521 atccataagc atcctgaatt agaaatgcaa ctaatctctc cagaacgtgc catagagctg
    29581 aaaaaagaag aggacctcct cgtgatggtg gattaccata aaccttctct atcgatctct
    29641 cagggcctat atgagcaatt tgataagatt gtgatcattg atcaccatcg tcgtggggaa
    29701 gagtttccaa gtaaacctct gctgacttat atcgaatctt cagcttcttc agcttcagag
    29761 ctagtttctg agctgatcca ataccaaagc agcaaaacaa atcgcttaga tcgttttgag
    29821 gccacattat tgcttgcagg gatggttgta gatacaaata gtttcaatgt ccgtacaact
    29881 gcacgaactt ttgacgttgc cagctattta aggtcttgtg gagcagacgc atccttagta
    29941 caatatttat tgagctcgga tttagcgaca tacttggaga tcagcaattt gattgctgga
    30001 agcgactatg tgacttctga tattgttgtg gcggcaggga cagacgaaaa agagtataat
    30061 agtgttatag cagcaaaaac agcagataca ttgctttcga tgatcaatat caatgcagct
    30121 ttcgtcatta caaagcggac agacggcttg attgggatca gtgccagaag taccggcacg
    30181 atcaacgtcc aaatcatcat ggaatcctta ggtggcggag gacacttcac aaacgcagct
    30241 actcaactgg aaaatacgac agttgaggaa acaaaacaga agctatttgc tgcaatcaaa
    30301 gaaaatatga atcaaatata tgataaggag taatccaaat gaaagtaatt tttttacaag
    30361 atgtaaaagg aaaaggtaaa aaaggggaca tcaaagaagt tccagcaggt tatgcacaaa
    30421 attatctaat caaaaataac ttggcaaaag aagcaacgaa aagcagtatc agtcagttag
    30481 caggacaaaa gaaagcacaa gaaaaacacg aagctgaaat cttggaagaa gctaaaaagt
    30541 taaaagaatt cttggaaaaa gaagatactg tagtcgaatt gaaggcgaaa gcaggggaag
    30601 acagccgttt gtttggttcg atcccttcaa aacaaattgc tgaagggtta aacaaacaat
    30661 acaaaatcaa attagataaa cgaaaaatcg aattagaaaa tccgatccgt tcactaggct
    30721 acacaaacgt acctgttaag cttcatcatg aggtgacggc tacgatcaaa gttcatgtcg
    30781 tacaagaata acaaatcttg atagcgatgt tcagctggta agtataacca gaaggaaaaa
    30841 agacaaaccg aaatttatct gagaaaacag ctgcagcaat gaaagcaaaa gctgcaaatc
    30901 gatttcagat tgatttcggt ttcttttttg ggaacagcaa aatagcataa agaaaacgag
    30961 tataataaaa gagaactatt tttttattac actttcggta cgaatgatga cttttctctc
    31021 attttcttgt aaaatgaagc aattgaagac aaggagcgat acgtatgaat gaagtgtggc
    31081 aggatcgaat cccaccgcaa aatattgaag ctgaacaagc ggttttaggc tctgtttttc
    31141 ttgacgcaga aacgatcatt gatgccatgg agtacataga gccaaaagac ttttatcgtc
    31201 gtggccatca aattattttt gagacaatga tcgagttaaa tgatcgtaat gaagcgattg
    31261 acgtggttac tgtcaaagac cggctggaac aagccaattt gttagaagat gccggtggtt
    31321 taagttatct ctcagacttg gcattagcag ttccgacagc agcaaatatc gtctactatg
    31381 cgaaaatcgt tgaagaaaaa tcattgctgc gtacattgat ccagacagca acaggaatcg
    31441 ttacgaaagg attcgagcaa ggagaagaag ttcaaacaat tttagatgat gccgaacgca
    31501 gtatcttgga ggtttcggaa aaaagaaatc gaagcggctt tttatctatt gctgaagtac
    31561 tgaactccac gattgaaaat atcgatcgac tagctcaaaa caatgaagaa atcacaggtc
    31621 taccaactgg ctatcaaggt ctggataaaa tgacagcagg tttccagccc gaagaactaa
    31681 tcattcttgc tgcacgtcca gcggtaggta aaacagcgtt tgcattgaat attgctcaaa
    31741 atgtaggaac aaaaacagat cgttctgttg cgatatttag tttggaaatg ggggcagaat
    31801 ctcttgtaaa tcggatgctt tgtgcagaag gttcgattga ggccagccat ttacgtacag
    31861 gtcagctttc ggaagaagaa tggtcgaatc tgatcgttgc gatggggagt ctttctcgag
    31921 caaatatttt tatcgatgat acaccaggga tcaaaatctc tgaaatccgt gcgaaatgtc
    31981 ggaaactagc gcaagaaaaa ggcaatctag gcttgatatt gatcgattat ttgcagttga
    32041 ttgaaggtaa cggtaaagag aaccgtcaac aagaagtatc agatatctct cgccaactga
    32101 aaaaactggc gaaagagctg aaagtacctg tcattgctct ttcacagtta tctcgtggag
    32161 tagaacagcg acaagacaaa cgaccagtgc taagtgatat tcgtgaatcc ggatcaattg
    32221 aacaagatgc ggacatcgta gccttcttgt atcgagatga ttattatgaa agagaaggcg
    32281 gagatgacga tgatggaaac gagccaaaaa atgacaacgt catcgaagtc atcatcgaga
    32341 agaaccgaag cggtgcacga ggaacagttg aactactgtt tatcaaagaa tacaataagt
    32401 tttcgtcgct ttcgccaaga gaagaatttt aaagaagagg ttgtgagcct tgagcaatcc
    32461 cgagagctct cgtaagaaga tgcaaggagt aaccgcagga acgactagag tacttaattt
    32521 aaacagatct ccaaagggat tgtgacaaaa gtcttgtcac agtccctttt tccgaataaa
    32581 cggtgttcaa tcagctgacc cttggtatta gctcagcaaa cgtcaaaata tgaggaacta
    32641 ttttgacgtt tgtttctcta atactcaggt caaaacgctg atcttacgac ctcttttcct
    32701 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatcatca aacatgagaa
    32761 gcagtttttt gaaattgctc cttgtgactc agagttaaac ggcaggctca caacctcttt
    32821 cttaaacggt gttcaaaagc ttgcatctgc ggtaatacgc ccaatcaaac gaaaaatatg
    32881 agaagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt ttcacaacct
    32941 ctgattcacg gtgttccaaa agtagcttct tcgaaaaata agtcgaaatt tcacaaaaat
    33001 ttgagaagca attttcgtac attccttctt atttcttgaa gctgaccact tctgttacaa
    33061 cctctttttt aataaccatt ctaaaaagat aaagttcgtt ttttaagcgt ttttataaaa
    33121 taaaaataaa aatcattcga ttttatttga aatatgtatc tattattgat acaatggttc
    33181 aggattaaat aatatagaac gaggtgttca catgtcatct gtagtagtag taggtacgca
    33241 atggggcgat gaagggaaag gaaaaatcac agactttctc agtgaaaatg ctgaagtgat
    33301 tgcacggtac caaggtggcg acaatgcagg gcacacaatt aagtttaacg gtgtgactta
    33361 taaactgcat ttgattcctt ctgggatttt ttataaagac aagatcagtg tgatcggaaa
    33421 cggcgttgta gtcaatccta aatcattggt taaagaatta gcttatttac atgaaaaagg
    33481 tgtagaaaca agtaatttgc gtatttctga ccgcgcgcac gtaatcttgc cttatcatat
    33541 caaattggac caattacaag aagatgctaa gggcgaaaat aagattggta caacgattaa
    33601 aggaatcgga cctgcctata tggataaagc agctcgtgta ggtatccgta ttgcagactt
    33661 attagataaa gaaatttttg aagaacgtct tcgtttgaat ctagatgaca aaaaccgtca
    33721 atttgtaaaa atgtttgatt cagaaccact tgattttgac gatatttttg aagaatatta
    33781 tgaatacgga caacaaatca aacaatacgt cacagatact tccgttatct tgaatgatgc
    33841 tttggatgca ggcaaacgcg tgttgtttga aggcgcacaa ggtgtaatgc tggatatcga
    33901 ccagggaact tatccttttg ttacttcttc taacccagta gccggtgggg taacaatcgg
    33961 aagcggtgta gggccttcaa aaatcaataa agttgtcggc gtctgcaaag cctatacgtc
    34021 acgtgtcggt gatggaccat ttccaactga attatttgat gaaacaggag aacaaatccg
    34081 cgaagtagga cgagaatacg gtacgacaac tggtcgtccg cgtcgtgtag gctggtttga
    34141 ttctgtagtt atgcgccatt ctaaacgagt ttcaggtatt accaacctat ctttaaactc
    34201 gatcgatgta ttaagtggat tggaaacagt aaaaatttgt acggcttatg aattagatgg
    34261 cgaactaatc taccactatc cagcaagctt gaaagagttg aaccgctgca aaccaatcta
    34321 tgaagaattg ccaggctggt cagaagacat tacaggatgc aaaacacttg cagaactgcc
    34381 agaaaacgca cgtaactatg tacgtcgtat ctcagagctt gttggtgtac gtatctcaac
    34441 attttctgtc ggacctgata gaacacaaac aaacatctta gaaagcgtct gggcgcaaat
    34501 ctaaggataa aaacgacgac tgaatcagga cgttcttacc agagcgagtc gaagtgttta
    34561 tctatacgat aataataact tgaagaggta gtgaaagaga cgtttagcct tattgccgat
    34621 aggctgtctc ttgaacactg tttattcaga
//
LOCUS       DANKMN010000025        34393 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000025 DANKMN010000000
VERSION     DANKMN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(44..1936)
                     /locus_tag="PW224_001146"
     CDS             complement(44..1936)
                     /locus_tag="PW224_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907852.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1933..3660)
                     /locus_tag="PW224_001147"
     CDS             complement(1933..3660)
                     /locus_tag="PW224_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907853.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3676..4326)
                     /locus_tag="PW224_001148"
     CDS             complement(3676..4326)
                     /locus_tag="PW224_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2907854.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4530..4826
                     /locus_tag="PW224_001149"
     CDS             4530..4826
                     /locus_tag="PW224_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2907855.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5018..5638
                     /locus_tag="PW224_001150"
     CDS             5018..5638
                     /locus_tag="PW224_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL2907856.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5730..6896)
                     /locus_tag="PW224_001151"
     CDS             complement(5730..6896)
                     /locus_tag="PW224_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2907857.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6908..7261)
                     /locus_tag="PW224_001152"
     CDS             complement(6908..7261)
                     /locus_tag="PW224_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2907858.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7389..8411
                     /locus_tag="PW224_001153"
     CDS             7389..8411
                     /locus_tag="PW224_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL2907859.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8430..8591
                     /locus_tag="PW224_001154"
     CDS             8430..8591
                     /locus_tag="PW224_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907860.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8850..9284)
                     /locus_tag="PW224_001155"
     CDS             complement(8850..9284)
                     /locus_tag="PW224_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907861.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9394..11253)
                     /locus_tag="PW224_001156"
     CDS             complement(9394..11253)
                     /locus_tag="PW224_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2907862.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11371..12531)
                     /locus_tag="PW224_001157"
     CDS             complement(11371..12531)
                     /locus_tag="PW224_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL2907863.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12838..14262)
                     /gene="araA"
                     /locus_tag="PW224_001158"
     CDS             complement(12838..14262)
                     /gene="araA"
                     /locus_tag="PW224_001158"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL2907864.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14278..14976)
                     /locus_tag="PW224_001159"
     CDS             complement(14278..14976)
                     /locus_tag="PW224_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2907865.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14933..16594)
                     /locus_tag="PW224_001160"
     CDS             complement(14933..16594)
                     /locus_tag="PW224_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2907866.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16812..18212
                     /locus_tag="PW224_001161"
     CDS             16812..18212
                     /locus_tag="PW224_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL2907867.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18464..19543)
                     /locus_tag="PW224_001162"
     CDS             complement(18464..19543)
                     /locus_tag="PW224_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2907868.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19629..20588)
                     /locus_tag="PW224_001163"
     CDS             complement(19629..20588)
                     /locus_tag="PW224_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2907869.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20600..22111)
                     /locus_tag="PW224_001164"
     CDS             complement(20600..22111)
                     /locus_tag="PW224_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2907870.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLKSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22133..22697)
                     /locus_tag="PW224_001165"
                     /pseudo
     CDS             complement(22133..22697)
                     /locus_tag="PW224_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(22703..23566)
                     /locus_tag="PW224_001166"
     CDS             complement(22703..23566)
                     /locus_tag="PW224_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2907871.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23580..24476)
                     /locus_tag="PW224_001167"
     CDS             complement(23580..24476)
                     /locus_tag="PW224_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2907872.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24651..25940)
                     /locus_tag="PW224_001168"
     CDS             complement(24651..25940)
                     /locus_tag="PW224_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2907873.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(25912..27429)
                     /locus_tag="PW224_001169"
     CDS             complement(25912..27429)
                     /locus_tag="PW224_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2907874.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27627..28517
                     /locus_tag="PW224_001170"
     CDS             27627..28517
                     /locus_tag="PW224_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL2907875.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28590..29414)
                     /gene="nadE"
                     /locus_tag="PW224_001171"
     CDS             complement(28590..29414)
                     /gene="nadE"
                     /locus_tag="PW224_001171"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL2907876.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29426..30898)
                     /locus_tag="PW224_001172"
     CDS             complement(29426..30898)
                     /locus_tag="PW224_001172"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL2907877.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31535..31897)
                     /locus_tag="PW224_001173"
     CDS             complement(31535..31897)
                     /locus_tag="PW224_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2907878.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32120..32872
                     /locus_tag="PW224_001174"
     CDS             32120..32872
                     /locus_tag="PW224_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2907879.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33012..33935)
                     /locus_tag="PW224_001175"
     CDS             complement(33012..33935)
                     /locus_tag="PW224_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2907880.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(33950..34222)
                     /locus_tag="PW224_001176"
     CDS             complement(33950..34222)
                     /locus_tag="PW224_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907881.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10148 a         7165 c         5911 g        11169 t
ORIGIN      
        1 cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa gcttcttctt
       61 cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc aatgtatcgt
      121 ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct gcatcgcgta
      181 ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg cggttcatcg
      241 ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca tcaaggatca
      301 ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc tgcccttgcg
      361 aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat ttacgaacaa
      421 aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt
      481 tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct tgcagtacca
      541 ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac
      601 gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc
      661 ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc aagttaaaat
      721 cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt
      781 atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct tcttcatcca
      841 acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc atcaaacttg
      901 caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct tgaacatccc
      961 ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg aacacatagc
     1021 ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat tgagctttcc
     1081 aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct ttttcagtat
     1141 gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg ttattcaata
     1201 aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct acgatcatca
     1261 cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc cagctgattg
     1321 aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat tgagtcaaat
     1381 tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac atgatatccc
     1441 catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct tccaaatcat
     1501 gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt
     1561 tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc gcgattttat
     1621 caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga gcgccagcca
     1681 ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt tggatttgga
     1741 agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata cgtttagaca
     1801 tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca ccttttacac
     1861 cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga gagggacgtg
     1921 tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga tttcttgata
     1981 agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt ttccttcatc
     2041 tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa ctaaaacggt
     2101 gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc
     2161 caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta atgcccgtgc
     2221 aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt ccacatggct
     2281 atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt
     2341 ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc ggatcgttcc
     2401 agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat tatattgagt
     2461 catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt catataatcg
     2521 agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga ttgcgacgat
     2581 ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg ctccatgata
     2641 tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt
     2701 aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa tacggtcagc
     2761 tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa agctgatcaa
     2821 gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag tcatatctgc
     2881 tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga tcaatgtcat
     2941 cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg tataatcttt
     3001 attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct
     3061 gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct tttgcatact
     3121 tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca ttactgggat
     3181 gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta tgctcgcgcc
     3241 aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa ccatttggat
     3301 ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt tatcaaacgc
     3361 atcttttgtc agactctctg attttttata gacatccgta cgcaattgct tacccagttt
     3421 ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac tgatcaaaga
     3481 aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt gcgctacccc
     3541 tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta cttggatcac
     3601 catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga tttttagcat
     3661 ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt tcaaattgaa
     3721 ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg
     3781 cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca attcatgatc
     3841 attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg catcttgttt
     3901 gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag cgacttttct
     3961 tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat tctcgccttc
     4021 caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata gatcaccatc
     4081 ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg tctcaaagta
     4141 ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc tagggatatc
     4201 cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag aaaactcgat
     4261 tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt
     4321 cggcatatgg attctccttt ctttcaagat gacacattgt catatgacgc tatgtcatta
     4381 taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat catccgatac
     4441 attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc ccattaaaat
     4501 agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc caagacttca
     4561 ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc ttagattaca
     4621 caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca agacaattaa
     4681 aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg cgatcagaag
     4741 cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg
     4801 aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga ggctgtgaca
     4861 aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta ttcttcaacc
     4921 ttatcaatat gacttgatag ccttttcttt ttcgagtaaa ctgattttgt tagtcatagt
     4981 tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa
     5041 aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac
     5101 aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga
     5161 tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga
     5221 attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga
     5281 tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc
     5341 aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac
     5401 agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg
     5461 cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt
     5521 aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc
     5581 agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa
     5641 attgagataa atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtgcc
     5701 ttggatattt tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct
     5761 tcataaagag aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct
     5821 gctgcataaa caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc
     5881 agcagaacta ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga
     5941 gagatcaagc gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc
     6001 atgaatggac ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct
     6061 aagtaaggga tcaggtttaa aaaacccgca ataatcccca acgtgacagc atactctaaa
     6121 ccaatgaccg caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt
     6181 cctcttatat aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt
     6241 tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat
     6301 agataaaata agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg
     6361 acaaaacttc caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc
     6421 gaattcatga acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg
     6481 tcccgcaaaa tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg
     6541 atgaagctca ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt
     6601 agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt
     6661 tcaaagtaat caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc
     6721 agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat
     6781 aaaaagacga tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt
     6841 aaaaaccagc gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt
     6901 cctcctgtta gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat
     6961 gaagatagat tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata
     7021 cgtgagctac tgtctttaaa ttcccattgg ccatcggacc gacataatcg atcgtatata
     7081 cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata
     7141 cttcttgaat caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca
     7201 gcatgggttc ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca
     7261 ttgcactctc tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg
     7321 tgtggaataa ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat
     7381 cagctcatat gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa
     7441 tctatacgat cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg
     7501 aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta
     7561 gtgtggatgg attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg
     7621 taacagagtc tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat
     7681 acggggcgaa ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac
     7741 tgaaagcagc agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc
     7801 atgcgcatgt ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg
     7861 gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta
     7921 ccttcactgc agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat
     7981 tcgcttattt atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa
     8041 caggtgcttt tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa
     8101 atggaggggc agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag
     8161 gacataactc gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc
     8221 aaatcatctc aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt
     8281 ttgtcgtgtg tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa
     8341 caggattact ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt
     8401 ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac
     8461 gggaagaact aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag
     8521 aggaatatca gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg
     8581 tagaaaacta atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt
     8641 ctatcctgat gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa
     8701 aggagtattt actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt
     8761 atcataaagg attttgatca ctctatcata aaggtaatat tacatttata ttagataact
     8821 ctagttttta ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt
     8881 tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat
     8941 agatcttcat ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct
     9001 acatatgaaa gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga
     9061 cgcaaagtac tgactttcat aagcattgcc cgatattttt ttgttccttc ttcaaataac
     9121 aggatgatct cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg
     9181 tttctgatca cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg
     9241 atttgatcga tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt
     9301 tctccattgt tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa
     9361 aaaatttaat ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc
     9421 taaactatat tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat
     9481 ctcttgttgc gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag
     9541 ttctcgttca aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt
     9601 tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc
     9661 atcgtgagtc gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc
     9721 atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg
     9781 atccccccaa aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg
     9841 cttcagtgcc gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc
     9901 acttccatct gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat
     9961 atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt
    10021 cattacttct ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt
    10081 ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc
    10141 tcttttatac atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt
    10201 taacgctgaa acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt
    10261 ggtgagttgc gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt
    10321 tgctgttcca ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac
    10381 ttcataataa tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc
    10441 tgagaaactt tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt
    10501 tttttcatcg gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata
    10561 tccttcaggt tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc
    10621 agtgttatag tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc
    10681 ttttcgcaac aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc
    10741 cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa
    10801 catgtaccag caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag
    10861 catagtgccg gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg
    10921 ataaatcggt tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat
    10981 ggctacgaca tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat
    11041 aagaataatc agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc
    11101 aaatttcaac gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact
    11161 aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg
    11221 tttggtcgaa agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata
    11281 aaagaatgtt gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa
    11341 gaattaatga aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg
    11401 gacctttcgt actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa
    11461 atcagtactg tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat
    11521 atctgcgatc gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt
    11581 tttcttttga taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg
    11641 ataattgacc gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc
    11701 caaacgtccg ctgtctgcca aagcttcctg agtcgatccc cctagatgag gcaacataag
    11761 tatacggtca ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg
    11821 ttgtttttcc aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt
    11881 gataagtaca gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt
    11941 atcttctgtc aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa
    12001 atctgcttgc cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat
    12061 ccctaagcta tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc
    12121 gatggttttg ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc
    12181 taaaatatta ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga
    12241 agataagagc aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt
    12301 attcatgacg atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac
    12361 accagctctt gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact
    12421 acgaataact attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa
    12481 cttttctatc tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc
    12541 tccttactaa caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc
    12601 ctagaagcaa aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac
    12661 aagttactgc cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata
    12721 aatggtgttc cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca
    12781 aatttcgtca atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta
    12841 tctaataacc actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt
    12901 tagatttagc gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc
    12961 ttcatggaaa ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc
    13021 agtcgttggt tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc
    13081 tgccatcgat acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc
    13141 ttctctatct cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt
    13201 cggatctact tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta
    13261 agtataatct tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc
    13321 aatcgctgca gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg
    13381 ttgtacagct agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa
    13441 agcgttatag cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac
    13501 ttttacatgt tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc
    13561 cttgtactct tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac
    13621 aagatctccg ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt
    13681 gtctccttct gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc
    13741 aaaactttca ttataggcta ctgcaacatc catccaatca gcaatctctt tttggatctc
    13801 aggtcgtttc cagtagccaa cgacgatttt attttgttta tttaatcgag cattgatgaa
    13861 cccatattcg cggtcaccat gagcggattg atttaaattc atgaaatcca tatcgatcgt
    13921 tttccaagga atgctttcgt tatattgcgt agctaaatga agcaatggtt tttgtagaag
    13981 ctttgtcccg cggatccaca tttttgctgg tgagaaagta tgcatccaag tgatcacacc
    14041 tgctacttct tctcgataat tgacctcttt catgattttt gtgatcgtat cagcagtaac
    14101 tgctaattct tgaaggcgga ttggataagg caattggcca ttttcattca gttcatcgac
    14161 catttcttgt gcatgtttct ttacttctcg cagtgcttct tccccatata aatgctgtga
    14221 tcctacaaca aaccaaaatt ctttttctcc aatattcaac ataattagcc actcctttta
    14281 tttgttttgt ccgtaatacg cattttttcc gtgttttcgt aaataatgtt tatctaaaat
    14341 ccgttgaggc agctcttctg aaaatgaatt gagctgtctg gtaaataaat tcatcttgca
    14401 tacttcatct agtacgactg cgttcattac cgcactttgc gcatctttcc cccaagtaaa
    14461 tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt tgcgttcttt
    14521 gaatgtttct atgataactt ttcctgtttc atattcgtac ccgctgtcga tttcctgttg
    14581 tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat
    14641 cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt gagtatggac
    14701 gattcctccg atttgaggaa atgaacgata taaaacagca tgagtaggcg tatccgaaga
    14761 gggattgagt ttaccttcaa tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc
    14821 tgtcatctct tcataactga ctccgctagg tttgataacg aaatatcgtg tttcaggatc
    14881 aaaagcgctc acatttcccc atgtatattt gacgagtccg tgtttaggca agtcaagatt
    14941 tgcttggtat acttcttctt tcagttgttc cagcattttc tatcactcct ttttcttgca
    15001 attgatgaat cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc ataaatatct
    15061 gatagcctgc tatttcttct gctgaaggtg tgatggtgat tccttggtct tgtccaaaca
    15121 cgtcttctgc caagaagttt tctaaagact tcccatgtcg atccatcata taggctgcta
    15181 ataacgcgat tccccacgct ccaccttctc cggctgtgtc catcactgta actggtgcct
    15241 ccatagcaga tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc
    15301 ccactaaacg atcaatttgg acattctcag atttcaatat atccattccg attctcattg
    15361 caccaaaagc actggctaaa tggatccgca tgaaattcgc taaatcaaaa cggctacctg
    15421 gtgtacgtac aaataatggc cttccttcat ttactcctgt aatgttttca ccggaaaaat
    15481 aaccataaga aagtaatccc ccgcaatccg tatcgccttc tagcgctttg ttgaataata
    15541 ccgtgaataa tttatctctg cttatctcca tacccaaaga attggtaaat tcttcaaaaa
    15601 tactgaccca agcattgata tccgaagagc agttattcgt atgaaccatg cctactagtt
    15661 ctcctgaagg tgtagtgacc atgtcgattt ccggatggac attttctagt tctttttcta
    15721 atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccct ttggctacac
    15781 tgttcgtagc gaccataccg gttcctgcat ccccttctgg cggacaaacc gggattcctg
    15841 gtctcaaatt tcccgtaggg tcgatcagat aagcccctga ttcgcttaac ttgccagctt
    15901 cttctcctgc tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt
    15961 tttcgattaa ctgaccaaaa atatttttca tttcttgatg gtaggttttc gttgaaggat
    16021 cgataggaaa catgcctgat gcatctccta cgcctagtac tttctcacca gtaagctgcc
    16081 aatggacata acctgcaagc gttgtcaaaa aattaatttc tttcacatgt tcttctttat
    16141 tcaaaataga ttgatacaaa tgagcgatac tccatctctg cggaatattg tattgaaatt
    16201 tttccgttaa tattcgttct gcttcttcag tgattgtatt tcgccaagta cgaaatggta
    16261 ctagtaactg ttccttgcta tcaaatgcta gataaccgtg catcattgca ctgaacccaa
    16321 tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa tacttctgct gccatttttc
    16381 ggtaactgac gcgcagtcct ttccaaatat cttctaatga ataggtccag ataccatttt
    16441 ctagtttgtt ttcccactca taactaccag cggcaatagg agtaaaagat gaatcaatca
    16501 atacagcttt gatcctagtt gatccaaact caatccctaa agatgtacga ccatctatga
    16561 catctaacat actttgcgca tttacttcct tcataatgat ttaactccct ccttctcgtc
    16621 aaacctgatc aaaccgtctt tttagtagcg cttacaatga ttattgtata cttttgtacg
    16681 tacatgtcaa gcagttattt acaagaatac aataaaaaac acttgttttg tacgtacata
    16741 agttataaaa tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg
    16801 agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat ttttttggcg
    16861 catttggcgg cattctattt ggatatgata ttggtgttat gaccggcgcc ttaccttttt
    16921 tacagcatga ttggggtttg gcaggtaaag cttctttgat aggatggatc acgtcttcgg
    16981 tcatgttagg tgcgatcctt ggcggctctt tatctggtct tctttctgat aagcttggac
    17041 gacgaaaaat gattcttctt tctgcactta tttttatggc tggttctgtc ctttctgcat
    17101 ctgcccctca caatggttct tattttctga ttgcagcaag gattcttcta ggtttagctg
    17161 ttggcgctgc atctgcccta gttcctgctt atatgtctga aatggctcct gctagactaa
    17221 ggggaagatt gtcagggatc aatcaagtga tgattgcttc tggcatgctc ctttcctatg
    17281 ttgcagatta tttgctaaaa gggttgcctg aaacgatggc ttggcgcgtg atgttagggc
    17341 tcgctgctgt ccccgcgctg attttatttt tcggtgtttt agctctacca gagtcaccgc
    17401 gttttcttat gcaatctggc cgcttggaag aggcaaaaag agtattgaac tatattcgga
    17461 caccaaaaga agctgaacag gaattcgaac aaatccagct gaatgtcaaa caagaaaaga
    17521 caaccggaac ttcttggcat actttgtttt tagaaaaata tcgttcctta gtcattgccg
    17581 gtattggtgt agcagtattc cagcaatttc aaggagctaa tgctattttt tactatattc
    17641 cgctgatcgt agaaaaagcg actgggcatg cagcgagcga tgcattgatg tggccgatca
    17701 tccaaggaat cattttagtt gctggttctt tgctcttttt ggttattgca gacaagttca
    17761 atcggcgaac tttattgaaa ataggcggta gcgtgatggg actttctttc atcctaccag
    17821 ctgttttagg aactgtactg gatgctcata caaattcgtt gttgattctc ttgtttttat
    17881 gtatttatgt cgcattttat tcttgcacat gggctccact gacatgggta atcgtaggcg
    17941 aaatattccc actagctgtc cgcgggcgag catctggcct agcctcttct tttaattgga
    18001 ttggttcttt cctagttggc ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa
    18061 tcgttttcgg catttttggg atcatctgct ttttaggggt tttatttatt caagagatcg
    18121 taccagaaac tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct aaaaaaactt
    18181 atcctaaacg tatttctatc catcattctt aaagaggttg tgacagaagt ggtcagcttc
    18241 aagaaataag aaggaaccgc ttagtttcca gtcacaagga acaatttcaa aaaacagctt
    18301 ctcatgtttg atgaatattg tgacttgtga ccagttggta ggagaacacc gtttagtgga
    18361 gaggtggtga aagaaacgtt cagccttgag cgatgacaaa agctctcaca aacattgcga
    18421 cgagtaaccg taggagcaat aagaggcggt aaaaacccgt ctcttatttt tcgttttctt
    18481 tcactgtttc ccgttcaact agtacaggtt catagagaat atttggctgt gcctgtcctt
    18541 tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc caacttttct tttggatgcg
    18601 tcaatgttgt caatttttta ttccctaaat ggcttaaaga agaatcgtct gttcctacga
    18661 tcgataggtc atctggaata tgatagccat tttctgtcaa cttatctagc attaaactag
    18721 ctatttgatc gttatagcaa atgatgccat ctaaagcttg ttcttctttc aaccgttgta
    18781 ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata tcttccgtca
    18841 aaaaacgcac attatactgt tcacacgcgc tgataaaacc tttcatcctg tattttcctt
    18901 gaagatcgtc gatttttgtg atgaacaaca gacgctgatg tccttttttt atcaaatatt
    18961 ctgctgccat aaaaccgact ttcacatcat ctaaacaaat ataagaggca tttaactctt
    19021 cataagcagc attgatcatc accatcggaa tgccttgttc tctcaaagca acataaagtg
    19081 ctaagtttgg gttatactga ttacttttcg taggctcaac gatcaaacca tctaccccat
    19141 aagcaatcat tttcttcaaa cactctttct ccaattcgtg atcgttattc gtactggcta
    19201 aaagcaatga atacccagat tctcgtaatt cattttcgat tcctcggata atcgacggaa
    19261 aaatataatc agagagatat gtggtgatga caccgatcgt ttttgttctg ttcaatggtg
    19321 ttttttcttt cttatagctg tcatctacat atgtgcccga ccctttttct ttacgcagat
    19381 agccctcatt taccaacaca gcgattgctt gccgaatcgt atgtctgctg acgtcatata
    19441 tttcttgcag cttgaattct gagggaatgg ttgtatttac ttcgtattcc tcacgaagga
    19501 tcttttgttt tatctcttct gcaatatctt gatattttgc catttgcctt gccccttttt
    19561 atttgtacgt acatatatca taacgagtct gcaaggttta ttcaagcaaa aatcaaggtt
    19621 tcttgtgctc ataaggcata cggaacaggt tcaccaaatt ctggaaatcc ttgctcattc
    19681 cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc ccctttttgg
    19741 ttcttctctg gtcgagcatg atagatcaaa atatcaaagc acccgtcttc tgaaaccgta
    19801 aacgagttgt gaccaggacc aaattgctta tttttttcag aagaaacgaa tactggctcc
    19861 gataatttat gccatgattc ttttaataaa agattcttgt gctcatcagc ccacagcagg
    19921 cccatggcat agttttcatc tgttgcacta ccagaatacg taatgaatat tttcccattg
    19981 cgagtaagga ctgctggacc ttcatttact gaaaaacccc gtttttccca ttcatactca
    20041 ggtatagaaa ggaggatctg cttccccttt aacgtccaag gattatccat ttcggataga
    20101 taaagattag aattcccagt aatttcagga tcttgttggg cccacacata atatagcttt
    20161 ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt ttttatttgt
    20221 cccttttcta tccaatctgt attcattgga tctatatgct gattttcaag tacgaacatg
    20281 cggtgatgat gagattcatc acgtatagtt ttatgattgc ttgcagcaaa gtaaatatac
    20341 cattttccat tgatataatg gacttcaggt gcccagatca attcactcat cggtccttct
    20401 tcatgagcct gccatacaat caggcttgca gcatgttcta attcattcag tgactttgcc
    20461 cttcttaatt caatcacttg gtagcctgga accgatcctg taaaataata ataaccatct
    20521 gtatgtttat atacccaagg atctgcccgt tgccgaacga taggattatt ataaagtgtc
    20581 ttcatcataa caaatttcct cacattttct ttattctaat tacattccaa gagtattttt
    20641 ctatccctac atttaattcg tcatgttttt ctattgaaaa atagtttatt tcaggtttta
    20701 ttttttctgg ttcttcacgt gtatttttgt ccaacagatc gctactggtt aatgtgtagt
    20761 gctctccttg atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt tgttcatttg
    20821 tgcgattgat caagaaaata gtcagctgat tgccgttatc tacgatcaca ctgtccacat
    20881 aaggaacatt ttttatcttt tcacaatcat aaacagggca aatgatttgt tgtttcaata
    20941 cttgaccttt agcatatttc gataagtgtt gcatcacata ataagttgga ttcctccatg
    21001 cttgtccttt agcatctgtc agtattaaag gtattgtgtt tactaacaat gattgacaac
    21061 taatctttac gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca aataataatg
    21121 tatcttccat agtaaagtaa caccaatcga tgggactacc gacttcaaaa tccctatgtc
    21181 ttttttccgg ttgggcatgt acattccatt catcaaacgc tagatacatt gttttctcac
    21241 ttttcaaata tccttttaca taatcacaag tagcaacgat ttcttcaatt tgacgatcga
    21301 atcgataagc ttttgcaagg tatgtcgctg tatcatcacg ctcattttta ggaggtttag
    21361 tcaagtcttc ttcatcatac ttatcaatat agttatgcaa agctaaataa tctacattct
    21421 cataagcttc ttctaatgcg atacgatccc attcagggta cgatggtaaa cgtggcgtag
    21481 agcttccaac taagattgtt tcaattgaat catccactaa cttcatgact tttgaggctt
    21541 catttgctaa cctaccatat tcataagcac tcttagcccc cacttgccat ggaccgtcta
    21601 gctcattccc taaacaccat aatttaacgc caaaaggctt tttttgccca ttttttattc
    21661 ttaaatcact ccagaaggtt ccttttggaa aattacaata ttctactaaa ttttttgctt
    21721 catcaattcc ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt acatgactga
    21781 tccatttaaa aaattcgtgg agtccaaaag aatttggttc tatacctcgc catgctaaat
    21841 caattttttt tggtcgttgt tctttcctac ctacagtatc cacccaatca taaccagatg
    21901 taaagtttcc tccaggatag cgaatgacac ctaattctaa ttcctttaca taagagagga
    21961 catcttgtct aaacccgtca ccatcagctg tttcatggcc cggttcataa atccctgtat
    22021 agacgacact tcccatatgc tcaacaaatg agccaaacag ccgagaactg atctctccta
    22081 ccgtaaaatc ctctctgatt tttactttca cttcttcgtc caacagcttt ccctaccttt
    22141 catttcctat agtcaagata atagatacaa gtttccatac gccaccagca aagataaaaa
    22201 taaaaggagg gaaaagaaat atgagcagta caaatcctaa taacaatacg caacaaagaa
    22261 tatttctcaa aaaatcaaaa aatactttcc atgcactttg ttcaaaaatt tgtaaaaatg
    22321 tgatatcttt gttttcagaa atcacatata tccaggataa aagaaacatg gaataaataa
    22381 gtaaaactac atatccacta attgaaagta cgattgtctg ttcttctaat aattgattaa
    22441 caagaaaata aataatgatc gttaacagac taaggaacaa gttcttacca aaatgttgct
    22501 ttattccttg aaagaaaaaa taaatgatat ttcctgatcc ctcacctttc cattgcgcca
    22561 ttacataaaa aaacagcaca tgtagcaggc acgatagtaa taacaggcaa acacacgatt
    22621 acccagagaa tacctaataa gatgtaatca gaaaccacaa tcaactgtcc ataaagttta
    22681 gtctcaacta ttttcatctt cctcactctt tcattccaga catagctgtt ccttgaacaa
    22741 aatatttctg tgcaaaaata taaaccacta gtaatggcag cattgaaata accgttccag
    22801 ccattacagg tccataaaaa gaaatgttct gccctgtaaa taaagctaac cctgctggta
    22861 aagttctcat ttttgaacta cttgtcaata tcaatggata aagcaagtca ttccaactat
    22921 tcatcagcgt gaaaatccct aatgataaaa gagtaggttt gctcaatggc aacatgattt
    22981 tcaaaaaaat attaaactca cttaatccat caatgcgtgc tgcctcttcc aaagaatctg
    23041 gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc atacgaggaa
    23101 tgatcaaacc tgcataagta tcaagcatgc caagcaaatt gacttcaata aatagaggaa
    23161 tcatgaagac ttggaatggg atcatcattg tcagcaaaac gaaatagaac aaaactgatt
    23221 ttcctttgaa acgcatacga gcaaatgcat accccgcaag agaatcaaag aatacagaag
    23281 tgatcactgt ccctccagca aaaatcactg tatttttgat gtaatctaat aaagggatcg
    23341 ttgtccaaac cttaatatag ttatctaggg tatagctatc tgcaaatatt ctaggcggaa
    23401 atgagataat gtctttttcg tgtttaaaag aagaaataat cagccacaaa aaaggaaacg
    23461 caataaccaa caatataact agcatactaa tcgttcgaat gattttttta ccactaactg
    23521 cttttcgact attcacagta actgtaatcg agctttctgg tgccatacag gtttccctcc
    23581 tatttcattt gtttttcttt ccgtgacatg tacacattag tggagatagc tatagttgca
    23641 acaatgataa acaaaaccga agacagtgct gatgcatagc ccaattcata aggtgctgta
    23701 aatcctcgac tgtaaatata ttgaacagct gtttctgtct taaattgtgg gccaccagcg
    23761 gttgcaacat atacttggtc aaacacttgc atcgaaccaa tcagattcgt taaaatacaa
    23821 aaacctaagg agggaacgat ctgaggtagc gtaatgttaa aaaactgctg tcgatttgtt
    23881 gcaccatcca tttgagcaga ttcatacaaa gagggagaga ttccctgaat agcagtcaag
    23941 agaatcgtca tagtaacacc aaaattcttc caaacagtca tgatggctac cgtcggcata
    24001 gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga aaacaagtaa
    24061 ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa taatcgcaat tgacgtcaat
    24121 gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct ggtattttta
    24181 gataatgcta ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc gagtacggtg
    24241 aaatataaag aatgaatcaa cgaattaact gctcgttcat cactaaatat acgtgtgaaa
    24301 ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt aaaactaata
    24361 gcaaatgttc ctaaaagcgg tatgacagta aataataata aaactaaaaa agatggagtt
    24421 aaaaacaaat acgcagcacg atcaggacga ttccaatatt tggtgaatcc gttcatatag
    24481 tccacctcat ctttcatttt gataaaatat acaatcttgg tttaaacgaa tctgaataag
    24541 atatccgaac aatcgctgtg atttttgcaa taacacctat ctgcaaatac taggtcgatc
    24601 attcagatat ctgtatcttg ccaacagtct atcctgtata aagttctgta ctatttttgc
    24661 agtaattgat tgatggcatc atctgcatcg ttcatgagtg tctcaggatc ttctcctgct
    24721 aataattttt caatcagtgg attgatgata tcgttattga tcgcagagat ttgggtaaaa
    24781 cctggtaaat atggctctgc atagtccatt tgtttcgata attcagatac aataggatca
    24841 ttttttactt cttcatcttt tgctactgaa tccaagtagg caggaaaacc attttcgata
    24901 ctccattttt taccaatttc agttgtattc caatatttca aaaactcata gacagcttct
    24961 tttttattat tatctgtact tgccggaatg gcatatccta cgccatctaa aatagctgtt
    25021 tttttgtctg aatcactccc ttgtggtaag gtagtcacat tataatcaat ctcgtttgct
    25081 ttcaatccgt tatttaacca tggcccattg atttcaatgg ctagttgacc agcattcatt
    25141 aaattgtcca tttcggcgcc agaaatcgat tctggaccag tcttgtctac atggatcatt
    25201 ttttggatcg cttttaacgt tttaaggttc tcaggagacg caaattcaga cttagtataa
    25261 tcatcgttca ctaatttacc gccatttgct aacatccagt tatataacgg tgcagttcca
    25321 tccttattga agacaaaacc tgatacattt ttactactat cagttaattt tggtgccatt
    25381 tctgtcaatt cttcccaagt agtaggtggt tgttcagtat ctaatccagc ttgtttaaac
    25441 aaatcattat tccaatacat atacatgcct tgtacctgca tggggacaaa atactgttta
    25501 ccttctactt cacctaattc aatagccgtt tctttaaaat cagatttatc tactccatca
    25561 tattcccaga aatcgtctaa tggttgtacc gctccgtttt ctacatactg tgcaaaatct
    25621 ggataattta atgcaataaa gtcaggtgct tttttagaag aaatcgctgt aggaagtttt
    25681 tcattcaaat tggcccaagt catgatatcc atttcaattt tgatgttttt atcatttgtt
    25741 ttattaaatt cattaacgat ttcttgcaat atttcaccat cagatgcagt aaacccattc
    25801 caaaaagtca aagtcacttc tttatcagaa tcagattctg ctgaacttcc gccaccgcaa
    25861 gctcctaaaa tacctgttgc agctaccaac aaagagccaa ttaaccattt tttacctttc
    25921 attttttgtt cctccttcat ctgataaacg acatctgacc acattccaag atagcggttt
    25981 caataaacaa ttgattttcc catcttcagc ttttacattt gttgaatgac ttggttttac
    26041 ttgttcactt ttaggaagat tagttgcttt tgtatcaaat ccactcattt cagaaaaatc
    26101 aatcactccc tctaatgata attcagacaa gtgaacagtt agattcattt cttcttctcc
    26161 acggttaact gcaaatatga caagttcttt gtcatttatt acggctatac tttctaaata
    26221 cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg ggcttaacac
    26281 ctctccttgt ccatacacag cagcttgcat aaatggataa tagatcgtct gtctccaagc
    26341 ttctccgttt gtttctgtcc tgattggtgc aataacattt accaattgag caagacaagc
    26401 aatctttact ctatctgcat gtttcaataa agtgattaat agacagccta atagtaatgc
    26461 atcttcaaaa ttatagttat cttctagtaa aggaggagca atctgccaag gcgctgcttg
    26521 ctcatcttgt tccgctgaat gataccaaat attccattca tcaaaggaca ggttgatctg
    26581 cttcttacta tgtttcttcg ccttgactgt atcgcacatc gctgtcactt cagaaatgaa
    26641 ccgatccatg tctaaggaac gtgcaagata atgagaaaga tcattttttt ggttgccata
    26701 atattgatgc agagacaaat aatctatttg atcgtacgct tgatcaagaa ctgttaactc
    26761 ccaatcacca aatgtaggca tttgccgatt cgaacttccg cataaaacca cctcaattga
    26821 atcatctacg agtttcatcg ccttagccgt ttcctctgct aaccgtccat attcttccgc
    26881 tgttttatgt ccaatctgcc aaggaccgtc catttcattc cctaaacacc atgtcttaat
    26941 tccaaaaggg ctctcttgac cattcgaacg ccttaaatca ctccaataag ttcctttagg
    27001 aaaattacaa tattcgacta aattacgagc tgcatctact ccacgagtac ccagattgac
    27061 agccatattg atttcggccc ccacatgctg acaccagtta taaaattcat gaatcccaac
    27121 ttcattcgtt tcaattgtcc gccaagctaa atccaacctt ctaggtctat ttgctatggg
    27181 cccaatactg tcttcccaac gaaatcctga cacaaaattt ccaccaggat atctaatcat
    27241 gggaatgttc aattctttta ccaagtgaat gacgtcttgg cgaaatccac ttttattagc
    27301 tgatggatga tctggctgat aaatcccttc atatactgct cgtcccatat gttcaataaa
    27361 tgaactgtac aaccgcggat caactttgcc tacacgcttg acactactaa tcaacattgt
    27421 ggcttccata actgacctcc tttaatttat acatgaataa tacacctcgt tgaaagcgtt
    27481 gtcaataata taaatgttaa acttacttat tttttatcga ttttaattaa ccaaaatatt
    27541 aataatgatt aaaacaaata atttaatata acttttatta acaaatatgt taatatataa
    27601 gaaaaacctt ttaagaggtg atatatatgc aactacaaat aaacgaagaa tccttaccgg
    27661 tatatgaagc attagctagt aaaacaagga tcaagatcat ccagctactt tccaaaaaaa
    27721 agatgaatgt caaagatttg gcaaaagaac ttggagtgag cagtgctatc acgacgatgc
    27781 acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga aaaagtagga caacaaaaaa
    27841 tttcgagcct tcgtgtcgat aaaatcgata tcagttttcc agaaaaaata ttcaatgctt
    27901 ttgatacgaa agaaacatcg atacctattg gtcactatac aaattatgca attgaaccaa
    27961 catgcggttt agctacgatc catgatttta tcgggaaagt ggatgaacca cgctatttta
    28021 tggatcctcg aaggatggat gcccgcattc tctggtttac cagcggattt gtcgagtatc
    28081 aggcacctaa ttttttaaat acagaggata cattggagat gttggaagtt tctgtggaaa
    28141 tttcttctga atttcctttt tcaaatgaca attggccttc agatatcact ttcagcctaa
    28201 atggtgtgga gctaggaaca tggacgagcc ctggagattt tgctgatatc cgcggtaaat
    28261 atacgcctga ttggtatcca gataatctta atcaatacgg cttgctgaag acgatccgaa
    28321 ttaccaaaca cttgacgaat atgaatggag aacccttgtc aaacatcacg atcaacgaca
    28381 tccctaaaga gcaagacact tggcatttgc gaatcgaagt gaaagacgat gccaaacatg
    28441 tggggggctg tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa
    28501 aagtttacta cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct
    28561 atgaagggac ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt
    28621 ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga
    28681 gcgggaactt cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg
    28741 acttcatctg caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat
    28801 aatttttccg gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg
    28861 aataatggca aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt
    28921 tctgcagcat gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc
    28981 gtaatcatac gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct
    29041 gcttcgctta atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta
    29101 acatcgggct ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca
    29161 taaggaagac gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct
    29221 tccatcgtta gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct
    29281 agtacaaaag ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt
    29341 ttacgtattt cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct
    29401 gtctgcaggc tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt
    29461 tcattcgttc catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt
    29521 atttttgcgg attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg
    29581 tatattcttt gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga
    29641 tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca
    29701 ctggatggaa catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt
    29761 agtctccttc agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg
    29821 gagtagttac tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat
    29881 catcttcgat cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga
    29941 tcaattttgt cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa
    30001 tcgttgcttc atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat
    30061 ctaattgttc ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc
    30121 cttggaagtt gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg
    30181 atttcaacgt atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg
    30241 ccataaatgc ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc
    30301 tagctggtat cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga
    30361 tatatgccgc tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa
    30421 attccaatag tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca
    30481 acgtttggaa attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag
    30541 gcccttccac ttggattaat ggttcatttg caaaaactag ttctccttca cgagcagaac
    30601 gaactgttgc tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt
    30661 ctaatccacg caagtaagca atatcggaat cattgaacgt cagattctct aaataattga
    30721 ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt
    30781 aacattcaaa aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat
    30841 tgatttgata tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct
    30901 tgtattactc ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag
    30961 gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa
    31021 aatgaactcc atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga
    31081 atacttattt tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta
    31141 cttgataaaa taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga
    31201 acaaatataa ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat
    31261 acgtctttcc ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga
    31321 atttttttgg aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa
    31381 aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa
    31441 caaaaatggc gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg
    31501 agcaggttac cataaagatg attattagtc gttattaaat agaatccttc gtattttttt
    31561 gttctatcac tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct
    31621 cctgtttcac tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa
    31681 tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt attgatcatc acgacttgct
    31741 ttttatcata tgtgtctacc aatagcagtt gtgttcgatc taacgaaaat aatttgtaat
    31801 aattaatagc cgtattccca aaaaagaatt ccactacttt aggataaaat gtcttaatat
    31861 ttaaattact attcacaggt gtttcgatta gcttcatctc attcctcttt tctatatagt
    31921 cgatttagat ccaatcttaa gttatgttac catctttcct agcattaagg aatcggttac
    31981 aattgaacct ttaaagacaa aaggatttac cttttatcac ttaatagtat aacataattt
    32041 cgtgagaaaa aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca
    32101 tttgtcagga ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt
    32161 cagtccgaga accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa
    32221 ttcttcaggt tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt
    32281 ttatcgaaca atggcattca agttggatga tatccaagag gtcttagaca atccatcttt
    32341 tgaccaccag aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat
    32401 cgataccctc ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat
    32461 gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc
    32521 ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa
    32581 atggatgaat atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt
    32641 atttgatctc actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt
    32701 ccaagctcat aagaagtggt tctcttacag ctggcctgaa tatcagccaa aagcgcataa
    32761 aggactagga atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag
    32821 tggtcatggt gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac
    32881 atgatccaac aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt
    32941 tagattttta tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac
    33001 tttaaatata ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct
    33061 gtaagaaaca attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg
    33121 aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct
    33181 tgcagatagc tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca
    33241 actccaagct ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc
    33301 caaaaaacaa gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt
    33361 tttgagttat aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct
    33421 tgctgcttca aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag
    33481 cttgcctgtt ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc
    33541 aataaatcat ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct
    33601 gcaaattctt caagatacct tcgctctttt tctttatgat agcccgtcgg taaaagtgtc
    33661 actttgtaat cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac
    33721 ggatgattgg tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt
    33781 ctggcatttg cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt
    33841 ttttcctcat ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa
    33901 cctgtgagct ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa
    33961 gattttcttg taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc
    34021 cttcatgcga aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga
    34081 tttcattcat tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg
    34141 ttaatacgga tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg
    34201 ttatctctac ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg
    34261 aaacgcgtct catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt
    34321 attaccaaag tctcttattt taaattgaat acattataat gatactgtaa atatatcttt
    34381 attattagtg aat
//
LOCUS       DANKMN010000026        34092 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000026 DANKMN010000000
VERSION     DANKMN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(29..1261)
                     /locus_tag="PW224_001177"
     CDS             complement(29..1261)
                     /locus_tag="PW224_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL2907882.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="PW224_001178"
     CDS             1516..2085
                     /locus_tag="PW224_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2907883.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="PW224_001179"
     CDS             complement(2263..2703)
                     /locus_tag="PW224_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL2907884.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="PW224_001180"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="PW224_001180"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2907885.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="PW224_001181"
     CDS             3657..4580
                     /locus_tag="PW224_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL2907886.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="PW224_001182"
     CDS             complement(4805..5044)
                     /locus_tag="PW224_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2907887.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="PW224_001183"
     CDS             complement(5049..5468)
                     /locus_tag="PW224_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2907888.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /locus_tag="PW224_001184"
     CDS             complement(5695..6933)
                     /locus_tag="PW224_001184"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2907889.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="PW224_001185"
     CDS             complement(7040..7675)
                     /locus_tag="PW224_001185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2907890.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(8017..8652)
                     /locus_tag="PW224_001186"
     CDS             complement(8017..8652)
                     /locus_tag="PW224_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2907891.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="PW224_001187"
     CDS             9164..10306
                     /locus_tag="PW224_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2907892.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="PW224_001188"
     CDS             10299..10505
                     /locus_tag="PW224_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL2907893.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="PW224_001189"
     CDS             10584..11777
                     /locus_tag="PW224_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2907894.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="PW224_001190"
     CDS             11982..13121
                     /locus_tag="PW224_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL2907895.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="PW224_001191"
     CDS             13114..13914
                     /locus_tag="PW224_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2907896.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="PW224_001192"
     CDS             13914..14741
                     /locus_tag="PW224_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2907897.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14719..15453
                     /locus_tag="PW224_001193"
     CDS             14719..15453
                     /locus_tag="PW224_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2907898.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="PW224_001194"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="PW224_001194"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL2907899.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="PW224_001195"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="PW224_001195"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL2907900.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="PW224_001196"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="PW224_001196"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL2907901.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="PW224_001197"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="PW224_001197"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL2907902.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="PW224_001198"
     CDS             19038..21071
                     /locus_tag="PW224_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907903.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="PW224_001199"
     CDS             21115..22395
                     /locus_tag="PW224_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL2907904.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="PW224_001200"
     CDS             22605..23411
                     /locus_tag="PW224_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907905.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="PW224_001201"
     CDS             23423..24643
                     /locus_tag="PW224_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907906.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="PW224_001202"
     CDS             24633..26219
                     /locus_tag="PW224_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2907907.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="PW224_001203"
     CDS             26258..28396
                     /locus_tag="PW224_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2907908.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="PW224_001204"
     CDS             complement(28764..29747)
                     /locus_tag="PW224_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL2907909.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30125..31516
                     /locus_tag="PW224_001205"
     CDS             30125..31516
                     /locus_tag="PW224_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2907910.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31532..32572
                     /locus_tag="PW224_001206"
     CDS             31532..32572
                     /locus_tag="PW224_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2907911.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32591..33676
                     /locus_tag="PW224_001207"
     CDS             32591..33676
                     /locus_tag="PW224_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2907912.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33709..>34092
                     /locus_tag="PW224_001208"
     CDS             33709..>34092
                     /locus_tag="PW224_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2907913.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIK"
BASE COUNT        11057 a         5832 c         6716 g        10487 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt
    30121 atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga
    30181 tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat
    30241 cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat
    30301 tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt
    30361 gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc
    30421 gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct
    30481 tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa
    30541 aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc
    30601 aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat
    30661 caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga
    30721 aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac
    30781 atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat
    30841 tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat
    30901 catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt
    30961 agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca
    31021 aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg
    31081 accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc
    31141 tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt
    31201 aggaccacgt cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta
    31261 ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata
    31321 tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt
    31381 gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc
    31441 tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga
    31501 aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt
    31561 accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga
    31621 ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat
    31681 cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg
    31741 gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt
    31801 cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa
    31861 gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag
    31921 aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt
    31981 tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg
    32041 ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata atagttgtga
    32101 ggatcgaata tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa
    32161 agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa
    32221 caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag acgatattaa
    32281 taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta
    32341 tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga
    32401 tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac
    32461 aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt
    32521 cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga
    32581 ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga
    32641 gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt
    32701 gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa gattaggagg
    32761 ccgtattttt gttgtaccta atcctaagaa aaatattttt aaatatttaa tagaagtgaa
    32821 taagttacta aaaaaagaat ctttttctat agttcacaat caggtttatt ttggcggggg
    32881 aattaattta tggttagcca aaaagaatgg aatcagacag agaattgctc atagccatgc
    32941 aactgaagat ggaaaatctc aaaatattgt aatgaatgta ttgcgtaaat ttttaaccaa
    33001 attgttgtta caaaatgcaa cagactacct tgccgttagt caagaagctg gtgaaagtct
    33061 gtttcaaaac cacccctttg agatagttca taatggtatt gatttagagc tttatagtaa
    33121 aaattcagaa gctaaggtta ataagagaaa agaattggat atctcaatga gtacttttgt
    33181 tgtgggaaac attggacgat tagagaaaca aaaaaatcaa tcttatttgt tagagatttt
    33241 tttagaaata ctaaaaatga attctgattc ttacttgatt ttagtaggtg aagggtcact
    33301 tagaaataaa ctgaaagcca aagcagtaga attggatata gagaaaagag taaaattttt
    33361 aggagaaaga aacgatattc ccgagttatt atctgtgatg gatgtttttg taatgccttc
    33421 tctttatgaa ggtctgccaa tggttggatt agaagcacaa gcagcaaaat taaaattagt
    33481 cttagcagat actatttcca aagatacaaa gttaacaaat aatgtggatt ttgtatcatt
    33541 agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa gagccgtatg ctaataaaat
    33601 aacacaagaa ctaaagagtt atgatgtttc gtacactact gaacaaatgg agagtatata
    33661 tagtagagat cattaaaaat gttcaagata ttttaggagt gattattttt gaaaatatgt
    33721 tatttgatat tggcatacaa tgatattggc aacttaaaga gattggtcaa tcgattgaac
    33781 gaaaatgcag tttttattat tcatatagat aaaaaaactg atatagaacc atttattgaa
    33841 gcatttaaat catatgataa tgttgaattt ttatgtaata aaaaccgtgt taaaatttat
    33901 tggggtggtt tttccattgt acaagcagaa atcaatttag taaagagagc attacaaaat
    33961 gagaaatatt taaaatatgt attattatct ggtgctgatt atccaatcaa agataatgag
    34021 tatatttata attattttaa aaagaatagt tcagtggagt ttattcgtgg aatagatcta
    34081 gatcaaatta aa
//
LOCUS       DANKMN010000027        33979 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000027 DANKMN010000000
VERSION     DANKMN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..513)
                     /locus_tag="PW224_001209"
     CDS             complement(<1..513)
                     /locus_tag="PW224_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2907914.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFP"
     gene            623..1111
                     /locus_tag="PW224_001210"
                     /pseudo
     CDS             623..1111
                     /locus_tag="PW224_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1200..1592)
                     /gene="rpsI"
                     /locus_tag="PW224_001211"
     CDS             complement(1200..1592)
                     /gene="rpsI"
                     /locus_tag="PW224_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2907915.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1608..2051)
                     /gene="rplM"
                     /locus_tag="PW224_001212"
     CDS             complement(1608..2051)
                     /gene="rplM"
                     /locus_tag="PW224_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL2907916.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2570..3037)
                     /locus_tag="PW224_001213"
     CDS             complement(2570..3037)
                     /locus_tag="PW224_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2907917.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3157..4695)
                     /locus_tag="PW224_001214"
     CDS             complement(3157..4695)
                     /locus_tag="PW224_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL2907918.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4775..5461
                     /locus_tag="PW224_001215"
     CDS             4775..5461
                     /locus_tag="PW224_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL2907919.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5662..6189)
                     /gene="msrA"
                     /locus_tag="PW224_001216"
     CDS             complement(5662..6189)
                     /gene="msrA"
                     /locus_tag="PW224_001216"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2907920.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6273..6950)
                     /gene="pgmB"
                     /locus_tag="PW224_001217"
     CDS             complement(6273..6950)
                     /gene="pgmB"
                     /locus_tag="PW224_001217"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2907921.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6947..8075)
                     /locus_tag="PW224_001218"
                     /pseudo
     CDS             complement(6947..8075)
                     /locus_tag="PW224_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(8075..10258)
                     /locus_tag="PW224_001219"
     CDS             complement(8075..10258)
                     /locus_tag="PW224_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2907922.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10255..11280)
                     /locus_tag="PW224_001220"
     CDS             complement(10255..11280)
                     /locus_tag="PW224_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2907923.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11325..12152)
                     /locus_tag="PW224_001221"
     CDS             complement(11325..12152)
                     /locus_tag="PW224_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2907924.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12149..13216)
                     /locus_tag="PW224_001222"
     CDS             complement(12149..13216)
                     /locus_tag="PW224_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL2907925.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13242..14078)
                     /locus_tag="PW224_001223"
     CDS             complement(13242..14078)
                     /locus_tag="PW224_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2907926.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14075..14944)
                     /locus_tag="PW224_001224"
     CDS             complement(14075..14944)
                     /locus_tag="PW224_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2907927.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15014..16297)
                     /locus_tag="PW224_001225"
     CDS             complement(15014..16297)
                     /locus_tag="PW224_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2907928.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16311..17996)
                     /locus_tag="PW224_001226"
     CDS             complement(16311..17996)
                     /locus_tag="PW224_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL2907929.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18271..19296
                     /locus_tag="PW224_001227"
     CDS             18271..19296
                     /locus_tag="PW224_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2907930.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19891..23565)
                     /gene="rpoC"
                     /locus_tag="PW224_001228"
     CDS             complement(19891..23565)
                     /gene="rpoC"
                     /locus_tag="PW224_001228"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL2907931.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(23623..27240)
                     /gene="rpoB"
                     /locus_tag="PW224_001229"
     CDS             complement(23623..27240)
                     /gene="rpoB"
                     /locus_tag="PW224_001229"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2907932.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27968..28936
                     /locus_tag="PW224_001230"
     CDS             27968..28936
                     /locus_tag="PW224_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2907933.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28954..29859
                     /locus_tag="PW224_001231"
     CDS             28954..29859
                     /locus_tag="PW224_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907934.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29856..30662
                     /locus_tag="PW224_001232"
     CDS             29856..30662
                     /locus_tag="PW224_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2907935.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30798..31787
                     /locus_tag="PW224_001233"
     CDS             30798..31787
                     /locus_tag="PW224_001233"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL2907936.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32104..32628)
                     /locus_tag="PW224_001234"
     CDS             complement(32104..32628)
                     /locus_tag="PW224_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL2907937.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32915..>33979
                     /gene="scm"
                     /locus_tag="PW224_001235"
     CDS             32915..>33979
                     /gene="scm"
                     /locus_tag="PW224_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2907938.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         9599 a         7264 c         6376 g        10740 t
ORIGIN      
        1 cggaaagcca gtcttagtaa cgatcatatg aattttaaga ccataaaaat aagatttttt
       61 tgttgagtta tagccaactt tggctatttg attcaatagt tttgcttgtc tatttctaac
      121 tggtttacat aatggactag gaaaactatc tataattccg actagttcac ctttggtgag
      181 cttcttgata aaaaagtatc gaataatctt gatagtgtaa gctaagtttg aacttaagcg
      241 agtgaatcga ctcctactag gaaagtcacc attaggaaat aagacggaac aaaccgctct
      301 atacgtggct ctttggctag tatagccatt aattatgccc catataacac aagcaataat
      361 cactgtatcg cgttgcttca gttgatcagt atttcttcga tttttaatct tatctggtac
      421 acaattgtgg tatatgttag aaacagttct cataatttcc ttgaatgtta taaaagtatc
      481 tgtataatgt tcagtatatt ttgatgaacg catatgtagt cctcatttct tgggagtttt
      541 gagactacta tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg
      601 aaaaaattaa ctagcaccac gggttgattt taataaaaat acggttcgtc tcaaatatgc
      661 tttagataaa tatcaaagaa agacacatac aaaaggttgg aaagacaccg ttattcaggc
      721 accagaagtt gtgatgacat tattaagaga atggaaatca gctcaggctg aacaattgtt
      781 gaaactaaaa attatacaaa cacctgacca gtacctcttt acctattcca aaccttctgg
      841 agaagtaaat tgtcctgttc acgctgacta cttaaactat cgaatcaatg caataaaaaa
      901 aaggcatcct gaattggctc aattatctcc acacaaactt cgtcatacct atgcaactat
      961 agctagggaa ggtggagcaa atatgaatca aatctctaat gctttaacgc actcggatat
     1021 ttctactaca aaaatatacg tgaacacacc tgacgtagtt gataaatctg tttttgaagc
     1081 atttcaaaaa ggattgaata agtgtgatta acatgtgttc tcaaatgtgt tcttattata
     1141 aaaaaagaca ctttccaaat aattgaaaag tgtcctgaaa tattgatata aaaagaatat
     1201 taacgttttg agaattgtga agctttacga gcttttttaa gacctggttt tttacgttcg
     1261 accatacgtg cgtcacgagt aagtaagcca gcacgtttaa gagctaaacg gaagtcagga
     1321 tcaacttcta gcaatgcacg agcgatacca tgacggatag ctcctgattg tccagcgtat
     1381 cctccgccgt ttacgttaac aaatacgtca tatgcgcctt ttgtttcagt aacagcgaaa
     1441 ggttgattga ttacttcacg caagtcagca tgtggaatgt attcttcaac atctttttta
     1501 tttacagtaa ttttgccagt tcctggtact agacgtacgc gagcaactgc gtttttacga
     1561 cggcctgtgc cgatatattg aacttgtgcc aatgaaattc cctcctatta gattaagttt
     1621 gtgatgtcta aaacttctgg ttgttgtgct gcatgtggat gttctgctcc accgtatacg
     1681 ttcaattttg taaattgttt gcggcctaaa gtgtttttag gaagcatacc ttttacagaa
     1741 gtttcgatca aacgacgaga atttttagca cgcaattcgc cagcagtgat tgatttcaat
     1801 cctcctgggt agttgctgtg acggtaataa attttgtcag tcgctttttt acctgttaat
     1861 ttcactttgt ctgcattgat gacgatgacg aaatcgccag tatccaaatg aggtgtgaaa
     1921 gttggtttat ttttaccacg tagtacagat gcaacaactg ttgaaagacg tcccaatgga
     1981 acatcagttg cgtctactac ataccattta cgttctactt cgcctggttt ggccatatat
     2041 gttgtacgca cgttattttc ctccaattta agttctgatg tttgacatac acttcgagtt
     2101 tccggggctc tttgtggggc aaacaatacc atttaacata ttacccgcta atctagtcaa
     2161 tgtcaacgtt tttctgcttg attgaaaaat ttatatgaaa ctttacttct tttctttctt
     2221 ttatataaaa gggtcgtttc ctgtcaaaat gaaaaacaaa aagaggctgt gacaaaagtc
     2281 ttgtcacaac cccttatttt gaataaacgg tgttctcctg ccaactcctc ggtcacaagt
     2341 cacaatatcc ataaaacata agaagctgtt ttttaaaatt gtttcttgtg actcggagct
     2401 gaacggcagg cttacaacct cttggtaaac ggtgttcaat cagctgaccc tcggtattag
     2461 ctcaaaaaac ggcaaaatat gaggaactat tttgacgttt gtttctctaa tactcaggtc
     2521 aaaacgctga tttcacaacc tctttttcgg tcaagttcaa caacagtttt tacgcgtcga
     2581 tctctggcgc tttgcccaaa tgagcttgga tcatccagat acgtttttct gtggctgttt
     2641 tgaattcgat aaagatatct tgtgttggat cgtcgccttc ttctccagaa acttcgattc
     2701 ctttttggta aagatctgct agataacgat agcctgcaac aagtgtttcc aaacgttctg
     2761 gaatagtacg atcccagcgg ccaatttcat cagggatttt tgtgttatct gcaaattcac
     2821 gcaatgtaga ataaggtgaa ccgtctaatg tgatcaagcg ttcggatact tcatctaact
     2881 gagcgttcaa atcatccatg aactcatcca tcattgggtg caatgtaagg aaacctggtc
     2941 ctctcatgta ccaatgtgtt tggtgaacaa caacagacat ttggcttaga tctgcaacta
     3001 attgatttaa tacttctttg gtttgttgga atttcatctt tcttgcctcc taacattttc
     3061 ttacacttaa aataatagta gactttcaat gaaaagtaca acaaaatcat ttacctgaga
     3121 acgattttca gttcgctttt ttatccgtaa agccaatcat ttttcagaat cagctaattc
     3181 ttctcgcagt tgagcaaatt gctgataagt ctgtgctaaa tgcttttgtg tcgaaacaaa
     3241 taatggatag attttctgat attttttatg atcagtttct tgtactccaa cttgctcttt
     3301 caattctatc aattcctgtg cttcactgat actttggatc tttccgatgc ttctccagcc
     3361 taaaatcaca gctcctaatg cggatgcttc tgtgacagta gggaaaacaa gcgggtaacc
     3421 cagtacatca gccatcatct gacgaaaaat cggagattga gcaaacccgc ctgtagcacg
     3481 gatctctgtg agctgatttt cctggcaaac tccttttaaa atccgataaa ggttgagaca
     3541 gatcccttct agaatcgcct tcatcatttc tttttctgta tgttgccgct gtaaaccgat
     3601 aaaactgcca aacgcatctg cgtcccaaag cggtgctcgc tcaccaagta aatagggatg
     3661 aaaaatcaga ccattggcac ccgctggcac aaaagcgata gccgaaagca gtgcatcata
     3721 tggattttct cctttttctt tagcagattg aacgacttcc tgtaaaaatg tatgccctgc
     3781 ccaatcaaag atgccagcac cattgctagt cgctcctccc actacccaat gtttctgatc
     3841 cagaacataa caaaacgtct ccatttctgg atggaactga ggctgagtag taataaaacg
     3901 aagtgctccg ctcgttccta cggtcaaagc ggcgatcgta tcaccaaaag cacctaaccc
     3961 tagattagaa agcggcccgt ctgcccctcc caaaacaaat gatatatcat ttggcaaacc
     4021 tagttctgct gctgtttttg atggtagacc tgttacttga taggtggaag gaaagacttg
     4081 cggcaattgc tctattcgaa tgttcaagta gtcaagcact tcttctgccc attggaattc
     4141 atggatatca aaatatccag ttcctgaagc tgttccgtag tcgctgatca gttcgccaaa
     4201 caatctataa aagatatagt ctttgatacc gacaaaataa gcagtttttt tgaataattc
     4261 aggttggctg ttttgcagcc acttgatttt gttcaatggt gtcattggat gaatcggcgt
     4321 cccagtctgc cgataaaatt ctttgccaag cacggtatat ttcagttttt ctgcttcgtc
     4381 cgctgctcgg ttatctgccc aagtgatcat tcgagataac ggtgttttct cttcatccaa
     4441 caatatcagg ccatgcatcg cagaggagaa ggagacaaat tgaatgattg tgtctgaagg
     4501 caactgttta attacttctt ttataacggt gcagacacct tgaaagatca attcctgatc
     4561 ttgttctgcc atcccttgtg tttcttggat caaagggtag cgaaaataag cggatgctgc
     4621 ttcttgggct tcatctgtga aagcaactgc tttggtttgt gtggttccga tatctaatcc
     4681 gattgaaacg ttcatttttt cacctcaaat tttatgtatg tacaaaaatt tagtttaagc
     4741 ttgcgtatct tggttttgaa aagggggaga tggcgtgtta ctttatttta tcattggttg
     4801 ctgtatcgga tcatttttat gtttaaccgc ccagcggatt ccgttaggtc actctattat
     4861 ttatcctaga tctcattgtg tcaaatgctg tcgttcgctt tcttggtatg aattgattcc
     4921 gattctctcg atcatcgtcc aacgatttcg ctgccgctat tgcaggtgtc ggttgccctt
     4981 ctactatttg ttggcagaag ccttatgtgg tggtcttttt gcctggttct ttactttttc
     5041 ttccgcaaga aatttttcga cgttcatatg gatgctttcg gctttgctat tttcgttgat
     5101 ggatttattt tacttcatgg tccattctca cacgctattc tgttcttggg caattttatg
     5161 gattttttgg ttgcaggccg gcgtcttcca atggcaaagt gtgctgttaa cgtttatcgt
     5221 aggcggtgtg tgccttcgct atggtcaatc ctatctcgga gctggcgata ctctgttgat
     5281 gctttcttgg agtggtggct tgtctctaga agagttgctt cagatcttgt ttcttgctag
     5341 tctgtttgga atatccgttt tttctgttta ctttatctgt cataaaaaaa agttggaaaa
     5401 gctgcctttc attccttttt tgagcagtgc gttgctgatt gtccttcatc ttaggtattg
     5461 aaaaaaggtt gtgacaaacg acttgtcaca acctctgata tatagtgttc tcttaccaaa
     5521 tccttgtgac ttggagctag gcgacaggtt cacaacctct ccactaaatg gtgttcaatc
     5581 agctgaccct cagtattagc tcaaaatgct ggttttacaa cctctgatat acggtgttcc
     5641 ataagtagct ttttcggctt cttatttctt gacgccccaa tgtcgacgaa ggaaacctgc
     5701 acgtccagaa ccttctcgat aaagattgta atggagcttg tttttcttgt aataatcctg
     5761 atggtaatct tctgcaggat aaaattcgct tcttggttcg atttttgtca cgattggctt
     5821 atcgaatcgc ccgctgtctt gcaatgcttg ttttgaacgc tctgcaatat ttcgttcttc
     5881 ttcatcatta tagtagatca ctggacgata agaatcaccg cgatcagcga actgtcctaa
     5941 tgcgtcagtc ggatcggttt gctgccaata gatttccacc aactgatcat aagaaataac
     6001 ttctggatca tattcgatct ctactgcttc tgtatgccct gttgtgcctg ttgttacttg
     6061 ttcataagtt ggatttggta catgcccgcc agtataaccg gatgtcacag aaatgatccc
     6121 tggctgctct tcaaatggtt tgaccataca ccaaaagcat ccgcctgcga aaactgcttt
     6181 ttttgtcata taaaatcccc cttttttctc atcatacctt gatttccaaa aggaggataa
     6241 cgttttgctt gtgacagaaa aaacggaaac tattatatac gtgatacggc ttctgataaa
     6301 tgccaaaacg aacgtacttg atcaaaaacg gttaaatcag gaatcacata ttcagcagaa
     6361 ggaagattta tgttttcacc tattcccaat acatgcatgt taccagccaa agctgcttga
     6421 caaccagctt ctgaatcttc aatgaccaag catgcatttg gcggtacatt caattgctgt
     6481 gctcctttga gaaaaacttc tggatcaggt tttgcttttg atacttgcgt gccgtcaata
     6541 aggacatcaa aataacttgt caaattcgtt ttttctaaaa tgaggcgtgc atttttgctt
     6601 gctgatccca atccgatctt gatgtttgtt tttttcaagt actcaagaac agctattgca
     6661 ccaggcaaaa cggatgtttc atccattttt tgaatcgctt gaagatatag attgtttttt
     6721 tgctcagcaa aggctgattt ctctttttct gagaatttct gttccatttt tccaaatgac
     6781 agcaatcgct ctagtgactc ggttctggat attcctttca gttgctcgtt ttctttctca
     6841 gtgaatggaa tggacagctg gttagctaaa accagccacg cttcgtaatg ataacgagct
     6901 gtgtctacaa gaacgccatc taaatcgaat agtgctgctt tgattttcat cgattacctc
     6961 cttgaaaatg aacgacttga agtaaagatg cgatgttttt agctgtgcga tgaagattat
     7021 cagtggcttg ctgtaaagct tcttgtaaag gcacagctgc aggaaggatc ggaaaaacgg
     7081 ccgaaatgcc aacatctgct gctttttcgc ttcctttacc aacacttccg caaatagcta
     7141 tcacaggaac gccctctggt actcttctag caatcccaac tggcgctttt ccttttagac
     7201 tttgtccatc tagacgtcct tcgcctacga ttacgaggtc tgcttgctgt acccgtatcg
     7261 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
     7321 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
     7381 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
     7441 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
     7501 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
     7561 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
     7621 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
     7681 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
     7741 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
     7801 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
     7861 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
     7921 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
     7981 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
     8041 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
     8101 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
     8161 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
     8221 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
     8281 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
     8341 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
     8401 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
     8461 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
     8521 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
     8581 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
     8641 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
     8701 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
     8761 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
     8821 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
     8881 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
     8941 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
     9001 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
     9061 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
     9121 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
     9181 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
     9241 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
     9301 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
     9361 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
     9421 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
     9481 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
     9541 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg
     9601 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
     9661 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
     9721 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
     9781 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
     9841 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
     9901 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
     9961 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
    10021 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
    10081 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
    10141 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
    10201 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
    10261 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
    10321 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    10381 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    10441 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    10501 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    10561 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    10621 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    10681 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    10741 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    10801 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    10861 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    10921 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    10981 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    11041 ttcgaagtga aagcgattgg ctgtacagac actcacgata tcgggagaaa tctcattcag
    11101 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    11161 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    11221 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    11281 ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    11341 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    11401 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    11461 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    11521 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    11581 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    11641 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    11701 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    11761 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    11821 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    11881 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    11941 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    12001 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    12061 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    12121 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    12181 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    12241 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    12301 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    12361 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    12421 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    12481 ctcttaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    12541 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    12601 tgtaatcggc accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    12661 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    12721 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    12781 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    12841 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    12901 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    12961 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    13021 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    13081 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    13141 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    13201 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    13261 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    13321 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    13381 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    13441 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    13501 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    13561 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    13621 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    13681 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    13741 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    13801 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    13861 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    13921 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    13981 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    14041 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    14101 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    14161 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    14221 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    14281 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    14341 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    14401 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    14461 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    14521 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    14581 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    14641 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    14701 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    14761 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    14821 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    14881 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    14941 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    15001 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    15061 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    15121 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    15181 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    15241 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    15301 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    15361 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    15421 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    15481 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    15541 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    15601 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    15661 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    15721 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    15781 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    15841 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    15901 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    15961 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    16021 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    16081 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    16141 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    16201 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    16261 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    16321 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    16381 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    16441 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    16501 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    16561 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    16621 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    16681 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    16741 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    16801 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    16861 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc ctctttcttc
    16921 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    16981 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    17041 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    17101 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    17161 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    17221 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    17281 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    17341 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    17401 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    17461 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    17521 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    17581 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    17641 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    17701 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    17761 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    17821 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    17881 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    17941 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    18001 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    18061 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    18121 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    18181 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    18241 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    18301 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    18361 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    18421 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    18481 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    18541 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    18601 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    18661 acgatggatg aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc
    18721 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    18781 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    18841 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    18901 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    18961 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    19021 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    19081 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    19141 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    19201 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    19261 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    19321 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    19381 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    19441 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    19501 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    19561 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    19621 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    19681 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    19741 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    19801 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    19861 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    19921 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    19981 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    20041 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    20101 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    20161 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    20221 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    20281 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    20341 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    20401 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    20461 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    20521 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    20581 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    20641 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    20701 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    20761 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    20821 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    20881 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    20941 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    21001 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    21061 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    21121 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    21181 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    21241 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    21301 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    21361 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    21421 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    21481 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    21541 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    21601 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tcttttactt tgaatcgttt
    21661 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    21721 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    21781 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    21841 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    21901 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    21961 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    22021 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag aaggagtaac
    22081 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    22141 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    22201 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    22261 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    22321 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    22381 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    22441 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
    22501 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    22561 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    22621 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    22681 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    22741 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    22801 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    22861 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    22921 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    22981 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    23041 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    23101 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    23161 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    23221 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    23281 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    23341 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    23401 acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    23461 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    23521 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    23581 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    23641 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    23701 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    23761 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    23821 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    23881 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    23941 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    24001 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    24061 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    24121 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    24181 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    24241 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    24301 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    24361 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    24421 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    24481 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    24541 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    24601 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    24661 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    24721 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    24781 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    24841 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    24901 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    24961 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    25021 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    25081 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    25141 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac catcatgttt
    25201 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    25261 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    25321 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    25381 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    25441 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    25501 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    25561 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    25621 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    25681 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    25741 acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt ttgtgtgtca attcgccaag
    25801 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    25861 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    25921 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    25981 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    26041 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    26101 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    26161 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    26221 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    26281 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    26341 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    26401 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    26461 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    26521 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    26581 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    26641 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    26701 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    26761 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    26821 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    26881 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    26941 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    27001 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    27061 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
    27121 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    27181 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    27241 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    27301 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    27361 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    27421 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    27481 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    27541 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    27601 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    27661 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    27721 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    27781 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    27841 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    27901 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    27961 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    28021 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    28081 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    28141 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    28201 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    28261 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    28321 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    28381 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    28441 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    28501 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    28561 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    28621 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    28681 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    28741 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat
    28801 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    28861 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    28921 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    28981 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    29041 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    29101 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    29161 gggagtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    29221 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    29281 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    29341 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    29401 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    29461 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    29521 atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa cggctttgct
    29581 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    29641 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    29701 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    29761 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    29821 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    29881 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    29941 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    30001 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    30061 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    30121 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    30181 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    30241 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    30301 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    30361 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    30421 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    30481 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    30541 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    30601 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    30661 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    30721 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    30781 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    30841 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    30901 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    30961 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    31021 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg atgacttctg
    31081 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    31141 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    31201 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    31261 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    31321 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    31381 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    31441 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    31501 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    31561 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    31621 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    31681 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    31741 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    31801 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    31861 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    31921 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    31981 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    32041 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    32101 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    32161 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    32221 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    32281 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    32341 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    32401 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    32461 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    32521 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    32581 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    32641 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    32701 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    32761 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    32821 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    32881 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    32941 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    33001 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    33061 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    33121 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    33181 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    33241 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    33301 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    33361 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    33421 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    33481 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    33541 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    33601 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    33661 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    33721 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    33781 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    33841 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    33901 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    33961 tgatagctca gattcatca
//
LOCUS       DANKMN010000028        33263 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000028 DANKMN010000000
VERSION     DANKMN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(41..229)
                     /locus_tag="PW224_001236"
     CDS             complement(41..229)
                     /locus_tag="PW224_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL2907939.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="PW224_001237"
     CDS             499..861
                     /locus_tag="PW224_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2907940.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="PW224_001238"
     CDS             913..2595
                     /locus_tag="PW224_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL2907941.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDDIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="PW224_001239"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="PW224_001239"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL2907942.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="PW224_001240"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="PW224_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL2907943.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="PW224_001241"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="PW224_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2907944.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6430..7434
                     /locus_tag="PW224_001242"
     CDS             6430..7434
                     /locus_tag="PW224_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907945.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7435..8379
                     /locus_tag="PW224_001243"
     CDS             7435..8379
                     /locus_tag="PW224_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907946.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8379..9341
                     /locus_tag="PW224_001244"
     CDS             8379..9341
                     /locus_tag="PW224_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907947.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9368..10282
                     /locus_tag="PW224_001245"
     CDS             9368..10282
                     /locus_tag="PW224_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2907948.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10308..12089
                     /locus_tag="PW224_001246"
     CDS             10308..12089
                     /locus_tag="PW224_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2907949.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12437..13123
                     /locus_tag="PW224_001247"
     CDS             12437..13123
                     /locus_tag="PW224_001247"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL2907950.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13143..16724
                     /gene="smc"
                     /locus_tag="PW224_001248"
     CDS             13143..16724
                     /gene="smc"
                     /locus_tag="PW224_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL2907951.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16733..17542
                     /locus_tag="PW224_001249"
     CDS             16733..17542
                     /locus_tag="PW224_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2907952.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17553..18551
                     /gene="ftsY"
                     /locus_tag="PW224_001250"
     CDS             17553..18551
                     /gene="ftsY"
                     /locus_tag="PW224_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL2907953.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18738..21005
                     /gene="gshAB"
                     /locus_tag="PW224_001251"
     CDS             18738..21005
                     /gene="gshAB"
                     /locus_tag="PW224_001251"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL2907954.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21094..21546)
                     /locus_tag="PW224_001252"
     CDS             complement(21094..21546)
                     /locus_tag="PW224_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL2907955.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21696..22646
                     /locus_tag="PW224_001253"
     CDS             21696..22646
                     /locus_tag="PW224_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2907956.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22639..23598
                     /locus_tag="PW224_001254"
     CDS             22639..23598
                     /locus_tag="PW224_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2907957.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23595..24350
                     /locus_tag="PW224_001255"
     CDS             23595..24350
                     /locus_tag="PW224_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2907958.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24373..25326
                     /locus_tag="PW224_001256"
     CDS             24373..25326
                     /locus_tag="PW224_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2907959.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25970..26266)
                     /locus_tag="PW224_001257"
     CDS             complement(25970..26266)
                     /locus_tag="PW224_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDL2907960.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26621..26977
                     /locus_tag="PW224_001258"
     CDS             26621..26977
                     /locus_tag="PW224_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2907961.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26987..27886
                     /locus_tag="PW224_001259"
     CDS             26987..27886
                     /locus_tag="PW224_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL2907962.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28403..29134
                     /locus_tag="PW224_001260"
     CDS             28403..29134
                     /locus_tag="PW224_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2907963.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29279..31306
                     /locus_tag="PW224_001261"
     CDS             29279..31306
                     /locus_tag="PW224_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2907964.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31414..32511
                     /locus_tag="PW224_001262"
     CDS             31414..32511
                     /locus_tag="PW224_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2907965.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32818..>33263
                     /locus_tag="PW224_001263"
     CDS             32818..>33263
                     /locus_tag="PW224_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2907966.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        11139 a         5762 c         7122 g         9240 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tgatatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcgataa acagaaatta tgttgcaaag taaagcgatt
     6301 ttctatataa ttattaataa ttcattacac aaagataaga ataacctttg ttataaaacg
     6361 ctttattctg tgtaatgtcc ttttttatta agaaaaactt ccatttagga agcagagagg
     6421 ggtattcgat tgtccgataa ccaattatta gaagttcaga acctacatac aggattccga
     6481 ctcaaagatg actattacga tgcagtagat gacgtttcat ttactttaga caaaaacgag
     6541 attttagcaa tcgttgggga atctgggtgc gggaaaagta ccttggctac tacgattatg
     6601 ggattacttg atcctaataa cacaaaaatt acaggtgaga ttttatataa cgatctgaat
     6661 ttaatcgacc tgaacgaaac gctctataat aaaatccgcg gaaatgatat cggaatgatt
     6721 ttccaagatc cgttgtctgc attaaatcct ctcatgagaa tcgaagacca gatcaaagaa
     6781 ggactttcct atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc actagaattg
     6841 ctagaacaag tagggattcc taatcctgcg agagttggac gccaatatcc ccatgaacta
     6901 tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc
     6961 gtcattgcag atgaaccgac gacagcccta gatgtaacca tccaagcaca gattcttgat
     7021 ttactgaaag accttcagga agaaacaaaa acagggatca tcttgatcac tcatgactta
     7081 ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa
     7141 gtagcaggtg taaaagaact gtttgatcat cctaagcatc cttatacacg ctctttactg
     7201 aactctatac cacaagaagg aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt
     7261 ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat tccatggata
     7321 ccagcaagcg atcatgaaga agagcctatc cttcatgaag tcgcaccgaa ccacttggta
     7381 cgttgctcct gctataagca tttccacttt agagacgaaa aaggagaggt gtagatgaca
     7441 gaattaatca caatcaaaga cctgaaagtt cattacccta ttcgcagtgg attcttcaat
     7501 cgagtaacgg atcatgtata tgctgtagat ggtgtggatt ttattatcga aaaaggaaag
     7561 acatatggtt tagtgggaga gtctggctct ggaaaatcta ctacgggaaa agccgttgtg
     7621 ggattagaaa aggtaacagc cggacaaatc atgtatgaag gaaaagatgt aacgaagcga
     7681 agcaaccgca aaaaaatggg ttataacaag gatgtgcaga tgatcttcca agattcaatg
     7741 tccagtttga atcctaaaaa gcgagtgttg gatatcattg ctgaacccat ccgaaatttt
     7801 gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga tatcgttggg
     7861 atgccggaag atgcgctata taaatatcct catgaatttt caggcggaca acgtcaacgt
     7921 ctaggagtag cccgtgctgt tgctaccaat ccaaaactga tcgtagccga tgaacctgta
     7981 tctgctctgg atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa
     8041 gaatacggcc tcagctattt attcatttca catgatttag gtgtagtaaa acacatgtgc
     8101 gacaatatcg caattatgta caaaggtcga tttgtagaaa tcggtacaag agaagacatt
     8161 tataatgacc cgcgacatat ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat
     8221 gtagaaaata gggaaagcca caaagaaaat cgacgaagag tagaacaaga gtatatccaa
     8281 aaccaaaaag aatactatga tgcgactggt cgagtctacg atttgcgtac gatcacaact
     8341 acacacaaag tggcgttgaa agatggaggt gcaagctaat gtggaaaacg attctgcgac
     8401 gtgttttatt gatgatcccg caagttatta ttttaagtgt tctgatcttc ttattagccc
     8461 aagcgatgcc gggggatcca tttaccggtt tgatcaatcc aaatcaggat ccggcagtga
     8521 tcgaacagat gcgacaagca gctggcttga atgacccatg gtatgtccag tacttccgtt
     8581 ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa cgctctgttg
     8641 ctgacttgat cggcgaacgg atcatcaaca ctttatatct atcgatcttg acagttgtcc
     8701 ttacttattt gattgctgtg cctctgggga tgcttgctgg ccggtatcaa aattcaatct
     8761 tggataagat tgtcgtaatc tacaacttct ttagttttgc tgttccttta tttatcttcg
     8821 gcttgatcat gctatttgtt tttggttatc gtttaggctg gttcccaaca agtggatcac
     8881 aaacactagg tttgcaaggt ggatttatca ctcaatggat agaccgtttg aaatatattc
     8941 tgttaccatc aattacccaa gcatttttgg cgactgccgt cacaatccaa tatctgcgaa
     9001 gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac
     9061 cgacgaataa agtattcagt cgacatattt tccgaaatgc agcgttgccg atggcttctc
     9121 aaatggggta tgaaatcact tcgttgattg ctggttctgt agttatcgaa aagatttttg
     9181 gttatccggg agtcggaaaa ctatttattg attccatcgg ccaacgggac tataccgtga
     9241 tcacatcctt agtattgatt ttaggaatcg cgacgctagt ggggacatta ctttcagata
     9301 tcatcatgag tatcgttgac ccgcgtattc ggattcaata aaaccaatca gaaaggagaa
     9361 tggatcgatg agtgaaaaaa acaatacagc agttcaagaa agtattcctc ctatggggat
     9421 acgaatgatt gctcgagaat ttaaaaaaga taaaattgct attttttcat taggtttgct
     9481 agtggtttta ttattagtga tctttatcgg tgcgatgctg accgatcaag acaaagtgat
     9541 gtatgttagt atcttcgaca aatacgctgc accaggaaca gtttcgacac aaggacaaaa
     9601 gttcattctg ggagcagatg aaggcggacg tgatgtatta ggacaattga tcataggtgc
     9661 tagaaactct gttacgatcg gatttgcgat cacgatcctt acttcgatca tcggggttgg
     9721 attagggatc atctctggct actacggagg gatcattgac aacatcttga tgcggatcgt
     9781 tgattttatc atgattctac cgatcatgct gatcattatc gtcttcgtat cgattatttc
     9841 cagatacaat gtctggtcct ttatattcat catgagtgca ttttattggg tagctaaagc
     9901 ccgattattc cgaagcaaga cattatctga agcaagaaga gattatgtca gtgcctcaaa
     9961 aacaatggga accaatgatt ttaaaatcat gttccgcgaa atcatgccga atctaagttc
    10021 attaatcatt actaatttga cgatgaactt tgcggcaaac atcggtatcg agacaacttt
    10081 gactttctta ggtttcggct tgccggcaaa tgtacctagt ttaggtacat tgatcggtta
    10141 tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc tggttacccg catcaatcct
    10201 tattttagtt ttgatgttga gtatcaatta tgttggacaa gcattcaagc gttctgcaga
    10261 tgcgcgccaa cggttaggct agtatagaag cgaaggggga aataggaatg aagaaaacga
    10321 tttttggatt tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg ggcggaggta
    10381 cagatacctc tggaaacaac tctggaggca acggcaataa caaagaggaa ttaacttttc
    10441 caattgcaac aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg
    10501 caacggacag tcagtttaaa ggattgttcc agtgggagtt ttaccaagat tcatatgatg
    10561 cagcttttat ggctccttct catgaaccgc tatttgcaaa cgatgacgat ttcagaatca
    10621 cagacgacgg agctgcaacg cttgatctag atgaagatac gaaaaaagcg acaatcaaag
    10681 taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat gtgattttcc
    10741 cttacgaaat cattgggaat aaagattaca ctggtatccg ttatgacgac actttccgta
    10801 acatcgtagg tatggaagaa tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa
    10861 aaatagacga caaaacagtt gaaatcgaat ataaagaagt aaacccaagc atgcttcaat
    10921 cgggtggtgg tatctggtca tatgcagcac caaaacatac gttagaaggt gtagcaatca
    10981 aagatatgga atcaagcgat caagtacgta aaaatccagt gacttttggc ccttattaca
    11041 tgagtaatat cgttgcagga gaatcagtag aatacttgcc aaacgagcac tactggaatg
    11101 gtacgcctaa attgaaaaag atcacgatga aatctgtacc gatggcaagt gcaactgaag
    11161 cattgaactc taaattatat gatatgattt tccaaatgcc aacagatact tacgatacct
    11221 ataaagaaat tgctggctat acaaacttag gccgtgaaca aacttcttat acttatctag
    11281 gattcaagtt aggtaaatgg aatgcagata caaagagtgt ggaatacaat ccagattcaa
    11341 aaatggctga taaatcatta cgtcaagcaa tgggctatgc attagataat aatgcagtag
    11401 gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc attgattcct ccagtattcc
    11461 agtcatacca tgatgatgag atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac
    11521 tattagatga tgctggctac aaagatgtgg atgacgatgg tttaagagaa gacaaagatg
    11581 gaaataaact aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg caaccattgg
    11641 cagattacta tatccaacaa tggaaagaaa ttggtttgga tgtccaatac acaacaggtc
    11701 gtctgatcga attcaattct ttctatgata aattagaaaa cgatgatcct gaaattgatg
    11761 tttaccaagc tgcttggaat acaggaactg atccaaatcc aagtggacta tggggaccaa
    11821 atgctgcgtt caactacact cgttatgaat cagaagaaaa tacaaaattg atcaacgata
    11881 tcagttcaag caaagcattt gatgaagagt accaaaaaga agcatatcaa aaatggcaac
    11941 aatatgcatt tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa gtattgccag
    12001 tcaacgaccg tgtcaaagac tgggattggt cttatgatgt accaaaccca tgggcaaatg
    12061 tttctgttac agcagattca cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc
    12121 ctttttcggt tttttgatat ttttctgttt atttctctaa tattcaggtc aaggcgctga
    12181 tttcatgact tctccaataa acggtgttca aagtccagtt tctcggtatt aagtcaaatt
    12241 tatgcaaaaa tatggaaagc cattttcaca aaattttttc ttcatactcg aaactaaacg
    12301 aatttttcac aacttctcgt caatcactag gattattaaa gctttactta ccttttgacc
    12361 gaaccatttc aaatttgaca aaaaaccagt ataataagga agtatcgaaa aaatatccat
    12421 aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa actataacat
    12481 cgtttttcac gatctaaatt tgcttgaaca agcttttacc cattcatcct atgtgaatga
    12541 gcatcgcaat ctgcaattat cggataatga gcgacttgaa tttttaggag atgccgtttt
    12601 agaactgatg gtttctcagt atttattccg tttatatcct catttgccag aaggaaaatt
    12661 gacaaaaaca cgagctgcga tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg
    12721 tcactttgat caatatgttt tgttgggcaa gggagaagag aattcaggag gacgtacgcg
    12781 accagcattg ctttgcgacc tattcgaagc ttttttaggc gcgttatatc ttgaccaggg
    12841 attcgacacg acccatgcat tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc
    12901 tttttcacat gagatggatc ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga
    12961 tgtatcaatc gaataccgat tgatcaatga agaaggtcca gctcatgaac gagtattttg
    13021 gatcgaagtt tatgtagatg atcaattgat tggcactgga caagggaaat cgaaaaaatt
    13081 agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag tgagggaaag
    13141 ctttgtattt gaaacgaatt gaaatagccg gttttaaatc atttgcagac aagacagtca
    13201 ttgattttga aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga aaaagcaata
    13261 ttacagaggc tatccgctgg gtattaggag aacaatctgc taaaagtctg cgaggcggaa
    13321 aaatgccgga tatcatcttt gccggttccg atacccgtaa gcagctgaat attgcagaag
    13381 tcacagtgat tttagacaat agtgatcatt atctgccatt ggagtacaat gagatcagtg
    13441 taacccgccg ttatcgacgg actggggaaa gcgagttttt catcaataaa caaagctgca
    13501 gattaaaaga tatccaagaa ttgttcttag attctggatt aggaaaagaa tcgttctcaa
    13561 tcatttctca aggaaaagta gaagcgattt tcagtagtaa accggaagac cgtcgtggga
    13621 tctttgaaga agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga
    13681 agctttttga aacagaagac aacctcagcc gcgtccaaga catcatccac gaattggaag
    13741 aacagttaac tcctttggcc gcccagagtg aggcagcaaa ggagttttta cggctaaaag
    13801 aaacactgac tcaaaccgat gtctctttga tggttgctga aatcaagaca gcaaaaaaag
    13861 attgggacaa caaacaagca cagcttgcga aattcaatct agaattaggc aaactatctg
    13921 agtcgatcca agaacaagaa tccattttgg cgaagcagcg taaagaaaat gcgcaagccg
    13981 atcgtctgat cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa ctgaagcaga
    14041 cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag
    14101 aatatcaaac ttcattagca gaagcacaaa aaaaggttaa acattttgaa aagcttcaag
    14161 aaagcttgat gaaagcagca gcagaaaaag aaacagagat ccaaaaagct gaagcaaatc
    14221 tcataaaaac acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa ttattggcag
    14281 aattaagaga tcaatatgtt gatttgatgc aagaacaagc agctgtagga aacgaactga
    14341 agtatctaga acgtcaatat atacaagaaa cagcaaaaag caagcagacg ttagccaagc
    14401 aatcggaagt agaagcatca gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa
    14461 agcaggcgca gctaaaatca tctttaactg aaacaaaaga gaagttagaa atgatccaac
    14521 aaaacgggaa aaagttccaa gaagcattag caaaagaaca gcctaaaatg tatcaattaa
    14581 tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc caagaaaact
    14641 attttggttt ttaccaaggg gttcgattgg tcttgcagca taaacagcag ctttcgggta
    14701 ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta gctatcgaag
    14761 cagctttagg tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag
    14821 ccatcacgta tttgaaacag caaagaggag gaagagcaac attccttcca ctgacgacaa
    14881 tcaaaccaag acaattaccg gctcatatat tgacacaagc agcagctgta gaagggttta
    14941 tagggattgc cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc
    15001 tgctaggaac gatcctttta gccaaagact taactagtgc caatgcaatc gcgcaaacga
    15061 tccgctatca atatcgagtg gtttccttag aaggtgatgt gatgaatgcc ggaggatcca
    15121 tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat
    15181 tgaaacagtt gactagtgaa tttgaagaag cagataaaca actccaagca caagaaaaaa
    15241 aagttcagga gctgcaacaa gaaacagctc gtttagccga agaacaagaa gttttgcgta
    15301 cacgaggcga acaattacgt tttgaagaac aagaagcgac aaatcagcta caaaacatca
    15361 taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag acacgtgaac
    15421 tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga attgaaagac aaacaaacag
    15481 atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg
    15541 accagatgga agccagaaga gcacaagtac aaagtcaaaa agcacaagaa caagcagatc
    15601 ttgctgtctt gaaagagcag tttaatcatc tgcagatcca gctccgcggt gcccgagtcc
    15661 aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg acattgacag
    15721 cagacttttc tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt aacgaattat
    15781 ctgctcagcg agaaacatta aaagccgagt tggcaaaagc caaagagcag cgagatagaa
    15841 ggcaaaagga gatcgataag cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag
    15901 cacgactgtc ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa atgattttag
    15961 ataatcattt gacctatctg cagtcagaat atcagctgac atttgagaaa gcttcccaag
    16021 actatcaaga aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac
    16081 agattgaaaa actgggacca gtaaacttaa attcaatcga acaatacgaa caagtcagtg
    16141 aaagacatac attcttagca acacagcgag atgatctgtt agcagcgaaa aaccaattgt
    16201 ttgaaacgat ggatgagatg gatgatgaag tgcggacaag attcaaagaa gtatttgaag
    16261 ctatccgtca agaattcaaa attgtctttc caaatatgtt tggcggcggt aaagctgaat
    16321 tagtactgac ggaaccaact gatttgctga aaacaggtat tgagattgaa gtacaacctc
    16381 ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg agaacgagca cttacagcca
    16441 tcgcgttgct cttttccatt atccgtgtct gtcctgttcc gttttgtatc ttagatgagg
    16501 tagaagcagc gttagatgaa gccaacgtga aacgttttgg tcgttacttg agcgatttcc
    16561 aagatgacac acaatttatc gtagtcacac accgcaaagg aacgatggtc gcagcagatg
    16621 tactgtacgg agtgacgatg caggaatccg gtgtatcaaa aatcgtttct gtacggatgg
    16681 aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa
    16741 attagtagca atcgatttgg acggtacatt attaaatgaa caaaagctga tttcagatga
    16801 gaataaacaa gcattagcac aagcaaaaca gcaaggggtc aagatcgttc tatgtacagg
    16861 acgcccctta gctgcgatgg cgcattatct gcaagaatta ggactggtag atgaaggtga
    16921 ctacagcatc actttcaacg gcggtttggt acaaaaaaat gatactggtg aaatcatgga
    16981 aaagaaagtg atggaagtat cagatatcca acgcttatac aaactggctc agcaattgga
    17041 tctgccacta gatgttttgt ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa
    17101 gcaatccttg tacaatgtgt tgaataagct gctgcaattc caaccagcgg atataagcga
    17161 tattacagat gaatttgtgc ttaataaggc agtaatcgct tatgaccaag aagaattaga
    17221 tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg tatgagatca tcaaaacaag
    17281 aagtatgctt ttggaattta tgccaaaagg tgttacaaaa gcttatggta tttctttatt
    17341 ggcaaaagat cttggtttga aacaagaaga aatcatggcg atcggtgatg aggaaaatga
    17401 tttaccgatg atccaatatg ctggactagg cgtagcgatg gagaatgctg tgcctttcgt
    17461 caaagaagca gcagatttcg tgacagccag caatgaagaa aatggcgtag ccaaagtgat
    17521 ccaaaaattt gtattagagt agggggagtc aaatgggatt ttttgataaa atcaagcgtg
    17581 cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac gacaagggtt
    17641 tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc aattttcgaa
    17701 cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca gatgtaggtt
    17761 ttgaaacagc gatacgaatc accgaagcat tacgtcaaga agtgaaactc agaaatgcga
    17821 aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc tatgaagcgg
    17881 aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca gttattttgt
    17941 ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc catcagttca
    18001 aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct ggagcaatcg
    18061 accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggag
    18121 gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagacagag caggcagatg
    18181 ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg aacgaattag
    18241 aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa acacttcttg
    18301 tattagatgc aaccactggt caaaacgcga tggttcaagc aaaacaattc aaagaaacaa
    18361 cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc ggtatcgtac
    18421 ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt gaaggaatcg
    18481 atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa
    18541 aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag ctccttgttt
    18601 gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac ttatttgtaa
    18661 gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt agattcatat
    18721 gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc aatgcgcgcc
    18781 cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt gatcttgcag
    18841 gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat cacccctata
    18901 tccaaacaga ttttagcgaa acacaaacag agatgatcac ccctgttacc gattctattc
    18961 ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct ataccgaaag
    19021 aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac gaagagatca
    19081 ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta gcaaaaaaat
    19141 acgggaagcg aaaacagatg gtaagcggta ttcatttcaa ttttgaattt ggcgatgagt
    19201 tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa ttcaaaacag
    19261 aactttattt gaaaacagcg agaaacttta tgcgctatcg ctggatgatc acttatttat
    19321 tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat ccgcaagaac
    19381 ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt
    19441 cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa gaagggaaac
    19501 tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag
    19561 atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg aatccttatg
    19621 caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg atgtacatgt
    19681 tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg actcgaaata
    19741 acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa
    19801 ttcttgaagg aatgaagcaa atgctggttg aattggattg gttggattct ctttatttag
    19861 tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg aaactctatc
    19921 aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat caatattata
    19981 aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa ctttctactc
    20041 agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta gatgagtcgg
    20101 atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat gcaaacatga
    20161 caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta acgaaaaaag
    20221 tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc atggaagaag
    20281 cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct aaatcaacca
    20341 attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat tatcaagcag
    20401 gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt atgccgggaa
    20461 cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg cgagtccctg
    20521 ctaatgttat tggtgacagt atccgaacag tgaaagaact tgttgaagaa aaaaatagtg
    20581 accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta ggtgaattag
    20641 aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa gccaatcaaa
    20701 ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc gatatgactg
    20761 atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta
    20821 agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca actgataaaa
    20881 aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat gtttatcctt
    20941 ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat cctgaagtat
    21001 tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat caaaagcttt
    21061 ttttgcatgt aaaaatttat gtgttttttc gttttaaaat aaacgatccc agaagctttt
    21121 tttctttttt ggtttttctt tttcagctgg cgaagagttt tcttctgggt attttttttc
    21181 gatatcaatc acaggttcat ttactgcttc ttttgctgtt aagagcaatc cgaattcctc
    21241 aggatttgca gtgacgtaat cgttgactac agtgaaacgg ctattgtttt tcgttgctag
    21301 ctggatatat tggttttgga cagaaagggg tagagcccca ttcaagagta tggaagcatc
    21361 tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt tttggttgga tcatttcagc
    21421 aacagtcata ctaagatagc agcgttccct gaatgtccct aaatatttat gctgttcttc
    21481 agggttcact aaaggtgtac cataaagtcc cttatccaaa tggcttttta catcatcttg
    21541 tgccataaaa aataccctcc ttcttctctt tgtctttctt acgattatag catattccca
    21601 ataataccta aaacatatga aaaagcggtt gcttttgttc aacgattcac ttaaaataag
    21661 aatgaaaatc attctcaatt agaaaggtgg attggatgcg ctacggacga ctagtagcat
    21721 tattagttat tttaagtagt atttcattgt tcatcggagt atcgaatgta caattgtcag
    21781 aactgctcca gttagatggg caaagcttac aaatcttgct agtgactcgt ttgccacgaa
    21841 cgatttgttt gatccttgtg ggagcgacca gtagtatttg tgggttgatc atgcaacatt
    21901 tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac gatggatagt gcgcgtttag
    21961 gtattttagt ggctatgatt ttcctacctg gtgcatcctt gattgtccga tccttgacag
    22021 cattttgctt tgcttttgca ggaacattgc tttttttatc ccttacgcga ttgttcctcc
    22081 agaaaaatca attgatgctt ccgttgattg gagtcatgtt agggaatatc attggttcag
    22141 ttgctacttt ttttgcttat caattccagt tggtacaaaa tatgacttca tggcttcaag
    22201 ggaattttgc gactgtgata cgaggaaatt atgagttatt gtatgctgtt gttccattgt
    22261 tgctcttggt ttggctattt gcttaccgat ttacgatcat tggtctgggc gaatcgtttg
    22321 cagtaaatct gggaatagat taccagaaga tgcagttttt aggcattggc ttagtatcat
    22381 tggctagtgc agtcatgttg atcatggtag gaagcattcc ttttatcgga gtgatcattc
    22441 cgaatcttgt ctctagaata tatggagatc aagtgcacaa aacaattggc gtaactgctt
    22501 tagcaggtag tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt
    22561 atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc ctttatcttc
    22621 tgataagggg acgacgatat gcttaaaaaa aagccatcta tttttattgg tatgcttgtt
    22681 cttgttttat ttgttacagg aatgtacttg acgatccata catatggaaa ctggcatttt
    22741 gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct agctgtcagc
    22801 agtgccacaa tcagctttca gacaattacg cataaccagt ttttgactcc ttcagtattg
    22861 ggacttgagt cgttatatgt ccttgtccaa acggtccttt tcttctttat cggaggagtg
    22921 acgatgcttc agcagacagg tacagttttt ttcttattga acgttgggct gatggtcggg
    22981 ctgagtctat tgttattttt ctttcttttg aaaaaaatga gagcgaatct ttttttatta
    23041 ctgatgtcag ggatgatttt aggtacgctt tttggtagtg tcagtacctt tttacaagtc
    23101 atcatggatc cgaatgaata tgatttgctt caaggcagat tatttgcaag ctttggcaat
    23161 atccagagtc agtatctttt gcctgcttct ttgatcatta tcggctgctg gtacctgtta
    23221 tttcgatatc gacatgtgct agacgttttc catctcggaa atgaacaagc agtaaacctc
    23281 gggatagaca cagatcgttt tgaatttttg attttatgtt gtgtcagtct tttgacaggg
    23341 acatcaacgg ctcttgtggg tccagttact tttttagggt ttattgtcgc aaatatcagt
    23401 tatcgtgtct tttctactta tcgtcatggt cagctattta tcggaagctt tctggtagct
    23461 atgctgtttc tgacaggagg acagctcgca gtagagcaga ttttcggctg gaatacaaca
    23521 gtgagtgtcg tcgttgaatt tgtcggagga atttattttg tcacaaaatt gattcgcgaa
    23581 aggaagagta tcctatgatt gatttacaaa aggtcaataa actgtaccat ggaaaatcag
    23641 tggttaaaga tgtacagtta acgattcctg aaaagaagtt gactgctttt atcggtccaa
    23701 atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca aaagatacag
    23761 gtgaaatcta tttagaccat aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa
    23821 aattatcgat cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag
    23881 tagaatttgg acgatttcct tatacgaaag gaaaattgac gacaaaagac gaggaaatcg
    23941 ttcaggaatc actggaacac cttgggttag aagaaatagc ggatcaatcg attgacactc
    24001 tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt
    24061 atattttact ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc
    24121 agattttaaa aaatttagta caagaaatcg ggaaaacaat tgttattgtt cttcatgata
    24181 tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga cagttattta
    24241 cgcaaggaac gacccaagaa atgataaaaa aagatgtatt ggatgaatta tacgaaatgg
    24301 acatacggat ttgtgagatt gaaggccgca gattctgtct ttatttctaa aactaacatt
    24361 aggagagaag agatgaaaaa attagtagta ggtatcttga ttctagcatc atttggttta
    24421 gcagcatgtg gaaatagcaa caacaccagt caggcagaca caaaaaaaag cagcacacaa
    24481 actgaaacta cattgacgat aacagatagt aatggagacc aaatcgaggt gccaaataat
    24541 ccagaaaaag tcgtcgtatt cgacaatggt tctttagata cgatggatgc tcttggtgtt
    24601 ggagataagg tagttggagc agctacaagc agccttcctg agtatctttc atcctataaa
    24661 aaagtagaat cagcaggtgg aataaaagaa ccagacttag aaaaaattaa tcagcttcag
    24721 ccagatctaa tcatcatttc tggccgacaa agagattttc aaaaagatct aagtcaaatt
    24781 gctccgacca ttttcctttc tttagatgcg aaaaatccat gggaatcttt ccagcaaaat
    24841 gtcactgctt taggggagat ctttggtaag caagaagaag ctaaaactca attagaggaa
    24901 ttatctagtg caatcgatca gacgaaaaag aaagcagaag ctactgacaa aaaagcacta
    24961 gtcacacttg tgaatgaagg gcaattatca gcctacggat ctggttctag attcggtttc
    25021 atccatgatt tatttggctt tgaacaagca gatgatcaaa tcgaagcatc cactcacggt
    25081 caaagtgttt cttatgaata tgtgctggaa aaaaatccgg atatcttatt tgtagtagac
    25141 agaacaaaag ctattggtgg agacgacagc aaagatgata tttctgccaa tgaactggta
    25201 gcacaaacga atgctggtaa aaatcagcag atcatctccc ttgaaccaga tgtctggtat
    25261 ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt
    25321 aaatagcata aataacagag gattaaactc tcatactatg taacaataat gtttcttaca
    25381 ttcttaaata gcaagagcct atcctcaaag aagttatcaa aaaccatccg taaaatcgta
    25441 ttgatgattt tacggatggt ttttatttat ttgagaaatc ttttttcaac gtgaattcat
    25501 tatctacatc atacgggttg attctagaat ggggagtggc aaaaaatagt gtgatccatt
    25561 ttttcagcat caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg aaacttagat
    25621 aaaacttaaa tttagatatt tttattttgt accgatctct tctttaaacg atgttccatc
    25681 tgctgcttct cggcaataag tccactagac tagaaaatat gagaagctat tttctgtcca
    25741 gcgttcttat tactcaaaac aaatcgcagt tgtcacaatc tcttctaaac gatgttccat
    25801 ctgctgcttc tcggcaataa gcccactaga ctagaaaata tgagaagcta ttttctgtcc
    25861 agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa
    25921 tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg
    25981 tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct
    26041 tactccaccg ttttagtaat tgatagaaat aactctcttc tgctttgatt ttatactcgc
    26101 ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg
    26161 ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat
    26221 tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa
    26281 agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa
    26341 aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta
    26401 tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca
    26461 agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa
    26521 cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct
    26581 gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa
    26641 agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat
    26701 cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt
    26761 tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc
    26821 tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc
    26881 atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt
    26941 gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc
    27001 gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct
    27061 ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa
    27121 taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac
    27181 atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt
    27241 tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt
    27301 agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt
    27361 acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga
    27421 ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa
    27481 acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc
    27541 tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga
    27601 attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt
    27661 tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga
    27721 atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat
    27781 gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca
    27841 tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa
    27901 tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat
    27961 cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat
    28021 ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa
    28081 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
    28141 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
    28201 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
    28261 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
    28321 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
    28381 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
    28441 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
    28501 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
    28561 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
    28621 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
    28681 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
    28741 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
    28801 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
    28861 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
    28921 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
    28981 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
    29041 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
    29101 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
    29161 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
    29221 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
    29281 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
    29341 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
    29401 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
    29461 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
    29521 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
    29581 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
    29641 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
    29701 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
    29761 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
    29821 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
    29881 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
    29941 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
    30001 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
    30061 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
    30121 gcaaagaagt cttacttatg atttggaagg tgtcggtcgc tcttctcgga ttatacctga
    30181 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
    30241 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
    30301 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
    30361 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
    30421 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
    30481 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
    30541 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
    30601 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
    30661 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
    30721 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
    30781 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
    30841 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
    30901 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
    30961 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
    31021 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
    31081 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
    31141 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
    31201 tacagctcat cgtggaatca gaataaatat tcctgtagaa aaccaaagaa tcggaacctt
    31261 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
    31321 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
    31381 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
    31441 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
    31501 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
    31561 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
    31621 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
    31681 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
    31741 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
    31801 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
    31861 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
    31921 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
    31981 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
    32041 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
    32101 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
    32161 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
    32221 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
    32281 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
    32341 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
    32401 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
    32461 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
    32521 aacaaatagt aaatttttat tattgtagga tggttgggct ctttttataa caatataaaa
    32581 tataccattt tattaataaa aatgcatact agtaaatgat ataattctaa taacaggttt
    32641 gatcgcgaga aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga caggacgatg
    32701 tatattatac aaacaagaat cgctgacaac agtgactcgg atccaggaaa tcagttttat
    32761 ccccagctag ttggtgaatg catggaaagt aatatacgta gaaagagctg atgactaatg
    32821 aattatgagt tttttttaag caaagaagat cacagaagtt attcattact taaatattta
    32881 gaagcaagct caaatttatc agagtcaata tcgaatgttc aagaagagtt gtcgctatcg
    32941 acatttctct tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa gttcgatcta
    33001 gagaaaaatt tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat taatggaaaa
    33061 tgttctagca aagctctgtt atctcgttat ataacggatt ctttatctat gaaaatggta
    33121 ttggctttct ttcaggaaaa atatatctca gtagaaaatt ttgctaaaca gcatcacgtg
    33181 agttattctg tggcttataa agtattacaa agactaaaaa gaaatctaaa aaaatatcaa
    33241 attttttttg aaaagagaaa gtt
//
LOCUS       DANKMN010000029        32940 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000029 DANKMN010000000
VERSION     DANKMN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(510..1076)
                     /locus_tag="PW224_001264"
     CDS             complement(510..1076)
                     /locus_tag="PW224_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2907967.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="PW224_001265"
     CDS             complement(1076..2032)
                     /locus_tag="PW224_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL2907968.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="PW224_001266"
     CDS             complement(2032..2688)
                     /locus_tag="PW224_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2907969.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="PW224_001267"
     CDS             3058..5076
                     /locus_tag="PW224_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2907970.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5146..5352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5503..7917
                     /locus_tag="PW224_001268"
     CDS             5503..7917
                     /locus_tag="PW224_001268"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL2907971.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8265..9818
                     /locus_tag="PW224_001269"
     CDS             8265..9818
                     /locus_tag="PW224_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2907972.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10199..11983)
                     /locus_tag="PW224_001270"
     CDS             complement(10199..11983)
                     /locus_tag="PW224_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907973.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11998..13719)
                     /locus_tag="PW224_001271"
     CDS             complement(11998..13719)
                     /locus_tag="PW224_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2907974.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13819..14580
                     /locus_tag="PW224_001272"
     CDS             13819..14580
                     /locus_tag="PW224_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2907975.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15547..15815
                     /locus_tag="PW224_001273"
                     /pseudo
     CDS             15547..15815
                     /locus_tag="PW224_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16487..19888
                     /locus_tag="PW224_001274"
     CDS             16487..19888
                     /locus_tag="PW224_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2907976.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(20312..20482)
                     /locus_tag="PW224_001275"
     CDS             complement(20312..20482)
                     /locus_tag="PW224_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2907977.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(20676..20855)
                     /locus_tag="PW224_001276"
     CDS             complement(20676..20855)
                     /locus_tag="PW224_001276"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907978.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            21242..22354
                     /locus_tag="PW224_001277"
     CDS             21242..22354
                     /locus_tag="PW224_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2907979.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(22432..22725)
                     /locus_tag="PW224_001278"
     CDS             complement(22432..22725)
                     /locus_tag="PW224_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL2907980.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            22893..23207
                     /locus_tag="PW224_001279"
     CDS             22893..23207
                     /locus_tag="PW224_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL2907981.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            23228..25207
                     /locus_tag="PW224_001280"
     CDS             23228..25207
                     /locus_tag="PW224_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2907982.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            25220..25546
                     /locus_tag="PW224_001281"
     CDS             25220..25546
                     /locus_tag="PW224_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL2907983.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            25562..26080
                     /locus_tag="PW224_001282"
     CDS             25562..26080
                     /locus_tag="PW224_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907984.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            26080..27432
                     /gene="celB"
                     /locus_tag="PW224_001283"
     CDS             26080..27432
                     /gene="celB"
                     /locus_tag="PW224_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2907985.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(27516..29021)
                     /locus_tag="PW224_001284"
     CDS             complement(27516..29021)
                     /locus_tag="PW224_001284"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL2907986.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(29113..29703)
                     /locus_tag="PW224_001285"
     CDS             complement(29113..29703)
                     /locus_tag="PW224_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2907987.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            29882..31528
                     /locus_tag="PW224_001286"
     CDS             29882..31528
                     /locus_tag="PW224_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2907988.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            31577..32296
                     /locus_tag="PW224_001287"
     CDS             31577..32296
                     /locus_tag="PW224_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2907989.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            32775..>32940
                     /locus_tag="PW224_001288"
     CDS             32775..>32940
                     /locus_tag="PW224_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2907990.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADG"
BASE COUNT        10612 a         5765 c         6691 g         9872 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtagcc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaactcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
     8101 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
     8161 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
     8221 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
     8281 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
     8341 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
     8401 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
     8461 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
     8521 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
     8581 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
     8641 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
     8701 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
     8761 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
     8821 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
     8881 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
     8941 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
     9001 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
     9061 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
     9121 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
     9181 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
     9241 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
     9301 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
     9361 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
     9421 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
     9481 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
     9541 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
     9601 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
     9661 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
     9721 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
     9781 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
     9841 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
     9901 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
     9961 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    10021 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    10081 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    10141 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    10201 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    10261 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    10321 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    10381 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    10441 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    10501 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    10561 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    10621 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    10681 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    10741 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    10801 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    10861 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    10921 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    10981 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    11041 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    11101 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    11161 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    11221 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    11281 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    11341 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    11401 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    11461 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    11521 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    11581 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    11641 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    11701 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    11761 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    11821 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    11881 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    11941 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    12001 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    12061 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    12121 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    12181 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    12241 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    12301 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    12361 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    12421 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    12481 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    12541 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    12601 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    12661 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    12721 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    12781 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    12841 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    12901 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    12961 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    13021 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    13081 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    13141 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    13201 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    13261 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    13321 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    13381 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    13441 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    13501 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    13561 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    13621 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    13681 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    13741 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    13801 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    13861 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    13921 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    13981 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    14041 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    14101 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    14161 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    14221 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    14281 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    14341 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    14401 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    14461 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    14521 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    14581 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    14641 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    14701 tttttagagg aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga
    14761 tgtaattgtc ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc
    14821 atggtgaact gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag
    14881 atgagaatga taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt
    14941 acattattac aatatgttaa taaggcaaaa ataaatagat ggattgaata aacatatttt
    15001 ttaatcaaat agtccctact gtgactttcc tcagctatcg gtgatactac cgatagcatt
    15061 tttttgctta taaaataatt ataagtattt agagattttt cttctaacat tcttcaaaca
    15121 agaaaaatca caaaggttat tgttattttt atatattctt tttaagaatg attgtttttt
    15181 tatagaaatt ttaaaaaata aaacacgtta ttgatggata gttagtttgt tatttggtaa
    15241 tattatagaa gttgactggt agtgatttat ttttcgtcgt tttataaaaa tgttattttt
    15301 tggaaaaatt cacaaatatg taacgaatgt ttttctggaa tttgctgtgt taaaaagcag
    15361 tatgactaat ataaatacta ttgtcattta tgcaattttt tatggatagt aattttagag
    15421 gggaatcaat gaaggaagaa tagaagggaa aaatagttgc aaatacgcca gttagcaaaa
    15481 atagcaatac atcttcaagc tttaaaattc gagagaatca tcttggtagt ggaatcaact
    15541 acaacaaaag gattaaacca attaatagat agaattacca atggaaaaat agaaaaggta
    15601 gttattctat acaaaagttg tttaattcgc ttcggttatg aacttagtga aaccctttgc
    15661 aatatatggt acagaatata gaaattattg acattacaga aaatgatgaa gatttaatac
    15721 agattgtcac tatttgtaga taccttttac aaggaaaccg agccaataaa gctaagaaga
    15781 tgattaagga gttgatggaa gacgatacaa gttaagaaag tgaaactacg cccaaataaa
    15841 gagcaaaaag agtaattgga cgtggaaaat gcaagaaatg aaccatcgat tcggtggaca
    15901 gtttattagt aatgacggtt tgagaacatc catgacgaga atgaaggaac atcctaaatg
    15961 cgtttagttg aaccaagtgc caaataatat ggccaaataa gcgatcagtt atcgatgaat
    16021 cccaagtatt ctaaactata tattcatttt gatgctaagc attgatatat ttttttggga
    16081 ttgatgaagg ttaaccaata cagggactca ctgatgaaat tctagagtct gaccttggta
    16141 tcaacgattt agtgattgtt tctaatggaa aagtataaaa gtatataagt acattaacaa
    16201 aataactatt agggttacgc tccagcttgt gcaaggcact ggcaggagga atcgtgaaat
    16261 cataattatg gttaagccac acggacaact aaaagtcata caaagctatc gaatcaatca
    16321 acagaagaca atcggcaatc attagctttg tttacagctt acattgcaac gcttgagttg
    16381 aaccgaaaat acgagggagg taacgcagcc attttgagtt ggggaatctt tttagaaaat
    16441 tatcgggtac cttgttgttt agtcaaaatt ataaggggga gtaatagtga ggaacaaatt
    16501 tcataaactg ttaagtatgt ttctagttat gcttattgtg atcaatcaat ttgggaacat
    16561 aacggttcta gctgagtctt tagcagaaca gccagagcgt tcgctgacga tcgaagttga
    16621 tcaagaggag gctgctttgg gtgagcagtt ccagttgcga gtcagaggaa caccagacct
    16681 gctaaatgca ttggtcattg ataagagtga ggatttgatt gaaggggaag tgaagcaact
    16741 caatgattac gaacgtctga ttacttattc ttcccagcat tcaggtaaat acgaattgac
    16801 cgcaagaagc ggtgatattc aatcaaatac ggtctcaata gaaatcagtg taccagaaga
    16861 aagcacaagt gagtctttta atgtaccaga agaaagcaca agtgaatctt ttaatatacc
    16921 agaagaaagc ttaagtgaat cttttaatga agccagatca tctgagccat ctataacgca
    16981 ggaatctcag attgaaagac aagaggagct cttgaattat gacatcggca tctctgctga
    17041 gcgccccact ttgccgagca ggcatacaca aacgttaaag ggatacattc aaggaggcgc
    17101 tggcgatatt cttgaagaat atggggagat ggtcgtacga gttcctagag catcgattgg
    17161 cttaattgag ttcattagag tggaggtcgc tgatgattca cctttaaagc cactggcatt
    17221 ttaccaagaa ggcacagacg ttgtttttcc atttaccttt gattatgaag gattaaatga
    17281 agccgctcag tcgtatacat tcagtatcac gtttgaagtt gctttctttc aatcaggcgg
    17341 tactgagcca aataatgact ttcaagtgag tcttacttat gctgagcgag cagcaaacac
    17401 ggaattttca attacgccta ttccttcatc aagacccatt ttttcgaaat tatactatgg
    17461 ctctagagaa atcgacagcc aaggaaatct tgggaatctg ctgattgacg ggcaagacgt
    17521 gaccaacagt aataaattta ttttgcaggt aaattatgaa ggatcacgaa aagacggtgt
    17581 agtggttacc gatgtactac cgcaaggaac aactttggca ccgaatccga ctacatggag
    17641 ttccactgga aatagagaac ctatttcagg tggggttcgg atcgcagaag taacgaatcc
    17701 aggtgaagca aacgttagaa ctcattatgt ttccgaggaa tttgtggaca aagtatcttt
    17761 taataaggaa acgaatacac ttgttgtgga ctttggtaat ctagcagagg gtcaatcgta
    17821 ttttattgaa tatgctcttg aagtgaccga tgtcgacttg ttcaatgaag gaagacaaga
    17881 ggatgggttt tctcggaatc gagcgagtat gacctacaat aatcaggtaa tgagccgaga
    17941 ctaccgcttg agaatccctg aaagtaatct tccggcaatc tctttaagaa aaagtgtgga
    18001 caaaaatgtt atcaatctca atgaaaatga actaacgtat actctgcatg tctcgatgtt
    18061 ttctggaaca agaaatacgc ttgttattga agatgttttg gaagaaggca tggaatttgt
    18121 gtcatttcta tcagatattc cctctttcat tgatgccgaa gtcaataatg gaatagttcg
    18181 tttcagtgcg aataatacga ttggtggtcc gacaagtttt accctctctt ttaaggtcaa
    18241 tgtagaggcg tatgcggtcg gcgatcaaat tttaaacttt gcaacgcttg tgaatagtga
    18301 caatcgattg aatacgagta ctgtaagaac aaggaaaatc gatgggcgta ttaaagtaat
    18361 taaaactgat gagaatggcc aaaggctggc aggagcaaaa tttgaaattt tatcaagcga
    18421 ttcacaagtg atacaagaag gtataacggc agaaaacgga gaatttttaa gtgaaccgct
    18481 tcctgtaggt agctatcaag tccgagaaac agaagcaccg gaagggtatc ttttagatgc
    18541 gacggtacat gatgttttta taaatgaata ctccatagaa gaagtgacgt taaccattga
    18601 aaacatcctt gatacgggtg gtgttgaact aattaaacat gctgaaggca gtggtgaagt
    18661 ccttcaagga gctgtttttg agttacagaa ccgagaagga gaaacgctac aaaccggttt
    18721 aactacagga gaagatggaa aactggcaat tgacggttta gcaccaggag catatcagct
    18781 ggtagaaact caagcgccaa tagggtatga attagatgcg acaccaatcg agtttgagat
    18841 cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa aaccgactta caccaggcgg
    18901 tgtcgtgttg acaaagatcg atgaccaaag cggggaaatc ctgcaaggag ctgtttttga
    18961 gttacagaac cgagaaggag aaacgctaca aaccggtcta actacaggag aagatggaaa
    19021 actggcaatt gacggtttag caccaggagc atatcagctg gtagaaactc aagcgccaac
    19081 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
    19141 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
    19201 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
    19261 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
    19321 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
    19381 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
    19441 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
    19501 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
    19561 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
    19621 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
    19681 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
    19741 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
    19801 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
    19861 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
    19921 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
    19981 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
    20041 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
    20101 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
    20161 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
    20221 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
    20281 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
    20341 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
    20401 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
    20461 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
    20521 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
    20581 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
    20641 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
    20701 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
    20761 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
    20821 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
    20881 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
    20941 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
    21001 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
    21061 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
    21121 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
    21181 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
    21241 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
    21301 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
    21361 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
    21421 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
    21481 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
    21541 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
    21601 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
    21661 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
    21721 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
    21781 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
    21841 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
    21901 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
    21961 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
    22021 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
    22081 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
    22141 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
    22201 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
    22261 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
    22321 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
    22381 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
    22441 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
    22501 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
    22561 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
    22621 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
    22681 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
    22741 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
    22801 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
    22861 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
    22921 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
    22981 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
    23041 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
    23101 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
    23161 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
    23221 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
    23281 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
    23341 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
    23401 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
    23461 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
    23521 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
    23581 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
    23641 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
    23701 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
    23761 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
    23821 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
    23881 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
    23941 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
    24001 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
    24061 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
    24121 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
    24181 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
    24241 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
    24301 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
    24361 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
    24421 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
    24481 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
    24541 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
    24601 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
    24661 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
    24721 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
    24781 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
    24841 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
    24901 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
    24961 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
    25021 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
    25081 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
    25141 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
    25201 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
    25261 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
    25321 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
    25381 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
    25441 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
    25501 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
    25561 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
    25621 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
    25681 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
    25741 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
    25801 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
    25861 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
    25921 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
    25981 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
    26041 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
    26101 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
    26161 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
    26221 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
    26281 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
    26341 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
    26401 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
    26461 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
    26521 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
    26581 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
    26641 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
    26701 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
    26761 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
    26821 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
    26881 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
    26941 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
    27001 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
    27061 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
    27121 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
    27181 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
    27241 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
    27301 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
    27361 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
    27421 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
    27481 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
    27541 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
    27601 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
    27661 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
    27721 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
    27781 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
    27841 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
    27901 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
    27961 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
    28021 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
    28081 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
    28141 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
    28201 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
    28261 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
    28321 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
    28381 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
    28441 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
    28501 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
    28561 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
    28621 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
    28681 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
    28741 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
    28801 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
    28861 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
    28921 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
    28981 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
    29041 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    29101 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    29161 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    29221 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    29281 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    29341 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    29401 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    29461 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    29521 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    29581 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    29641 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    29701 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    29761 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    29821 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    29881 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    29941 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    30001 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    30061 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    30121 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    30181 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    30241 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    30301 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    30361 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    30421 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    30481 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    30541 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    30601 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    30661 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    30721 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    30781 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    30841 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    30901 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    30961 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    31021 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    31081 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    31141 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    31201 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    31261 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    31321 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    31381 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    31441 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    31501 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    31561 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    31621 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    31681 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    31741 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    31801 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    31861 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    31921 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    31981 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    32041 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    32101 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    32161 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    32221 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    32281 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    32341 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    32401 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    32461 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    32521 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    32581 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    32641 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    32701 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    32761 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    32821 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    32881 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
//
LOCUS       DANKMN010000030        32534 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000030 DANKMN010000000
VERSION     DANKMN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            133..507
                     /locus_tag="PW224_001289"
     CDS             133..507
                     /locus_tag="PW224_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2907991.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="PW224_001290"
     CDS             complement(621..1250)
                     /locus_tag="PW224_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2907992.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="PW224_001291"
     tRNA            complement(1384..1456)
                     /locus_tag="PW224_001291"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="PW224_001292"
     tRNA            1670..1743
                     /locus_tag="PW224_001292"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
     gene            complement(1881..2045)
                     /locus_tag="PW224_001293"
     CDS             complement(1881..2045)
                     /locus_tag="PW224_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2907993.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2340..3668
                     /locus_tag="PW224_001294"
     CDS             2340..3668
                     /locus_tag="PW224_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL2907994.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3870..4703
                     /locus_tag="PW224_001295"
     CDS             3870..4703
                     /locus_tag="PW224_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL2907995.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4719..5456
                     /locus_tag="PW224_001296"
     CDS             4719..5456
                     /locus_tag="PW224_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL2907996.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5611..6579)
                     /locus_tag="PW224_001297"
     CDS             complement(5611..6579)
                     /locus_tag="PW224_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL2907997.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6811..7644
                     /locus_tag="PW224_001298"
     CDS             6811..7644
                     /locus_tag="PW224_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL2907998.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7678..8517
                     /locus_tag="PW224_001299"
     CDS             7678..8517
                     /locus_tag="PW224_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2907999.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8543..9103
                     /locus_tag="PW224_001300"
     CDS             8543..9103
                     /locus_tag="PW224_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2908000.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9302..11023
                     /locus_tag="PW224_001301"
     CDS             9302..11023
                     /locus_tag="PW224_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL2908001.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11188..12330
                     /locus_tag="PW224_001302"
     CDS             11188..12330
                     /locus_tag="PW224_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2908002.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12346..13557
                     /gene="thiI"
                     /locus_tag="PW224_001303"
     CDS             12346..13557
                     /gene="thiI"
                     /locus_tag="PW224_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL2908003.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14130..14777
                     /locus_tag="PW224_001304"
     CDS             14130..14777
                     /locus_tag="PW224_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2908004.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14906..15122
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15170..17815
                     /locus_tag="PW224_001305"
     CDS             15170..17815
                     /locus_tag="PW224_001305"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL2908005.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18125..19438
                     /locus_tag="PW224_001306"
     CDS             18125..19438
                     /locus_tag="PW224_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL2908006.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19428..20081
                     /locus_tag="PW224_001307"
     CDS             19428..20081
                     /locus_tag="PW224_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2908007.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20136..20827
                     /gene="radC"
                     /locus_tag="PW224_001308"
                     /pseudo
     CDS             20136..20827
                     /gene="radC"
                     /locus_tag="PW224_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            20977..21177
                     /locus_tag="PW224_001309"
     CDS             20977..21177
                     /locus_tag="PW224_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2908008.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21431..23110)
                     /locus_tag="PW224_001310"
     CDS             complement(21431..23110)
                     /locus_tag="PW224_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2908009.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23112..23324)
                     /locus_tag="PW224_001311"
     CDS             complement(23112..23324)
                     /locus_tag="PW224_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL2908010.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23717..25447
                     /gene="efrA"
                     /locus_tag="PW224_001312"
     CDS             23717..25447
                     /gene="efrA"
                     /locus_tag="PW224_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL2908011.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25444..27204
                     /gene="efrB"
                     /locus_tag="PW224_001313"
     CDS             25444..27204
                     /gene="efrB"
                     /locus_tag="PW224_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL2908012.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27299..27496
                     /locus_tag="PW224_001314"
     CDS             27299..27496
                     /locus_tag="PW224_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908013.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27649..28134
                     /locus_tag="PW224_001315"
     CDS             27649..28134
                     /locus_tag="PW224_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL2908014.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28238..28831)
                     /gene="clpP"
                     /locus_tag="PW224_001316"
     CDS             complement(28238..28831)
                     /gene="clpP"
                     /locus_tag="PW224_001316"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL2908015.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29011..29538
                     /locus_tag="PW224_001317"
     CDS             29011..29538
                     /locus_tag="PW224_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908016.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29629..30366
                     /locus_tag="PW224_001318"
     CDS             29629..30366
                     /locus_tag="PW224_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2908017.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30370..31287
                     /gene="miaA"
                     /locus_tag="PW224_001319"
     CDS             30370..31287
                     /gene="miaA"
                     /locus_tag="PW224_001319"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL2908018.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31289..32518
                     /gene="hflX"
                     /locus_tag="PW224_001320"
     CDS             31289..32518
                     /gene="hflX"
                     /locus_tag="PW224_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL2908019.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10771 a         5522 c         6875 g         9366 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaaataa agcttatgca aagaaaaaga aatccaatca cttgggtttc tttttctttt
     1801 attattatga tgaatcaagc tttctgtttt tgtcacgtat gttgtaatct ttacgaaaga
     1861 gtagagttgg ctactcataa tcaaccatac ttcccatcat cgctaaatac gaaataaatc
     1921 agcattccta caccaatcaa agcacaagct gcaccaagaa aagggatgac attcccaaag
     1981 ttatctgtat tcataagtat gccaccaccc acaaacaaaa agccgccagc aatcaggttt
     2041 ttcattttat tcatctcctt tcttacgtta tagtatttcc ttctttcaat gagtattttg
     2101 aaagctatct tattgtatga tgttcttcta ggcctcttct ttattataca ggccattttc
     2161 gttatctcat agaaatattc ctttttatct gaaaactatt gtataccgta tgacattttt
     2221 cttcatgaag ttgtaagtat attctcattc aaatgaaaat ttcatcatag aagagattgt
     2281 atttctaaat tcttaagagt aaaatacgaa gtataacttg gatttggaag gatgattgga
     2341 tgaaagctat tacgcctgat atgacaacaa aatttaacaa acagtttcaa gcaaatatca
     2401 aacagcaggc attgcagcgg gcagttgtga agaacggaat tactgcttct gctgaaaatg
     2461 tttcagcaca tgtggagaat acaccggtat tttctataga tttggctacg gggaaagtgg
     2521 ctaatcaaat gcaatcgggg cgttgctgga tgtttgctgc actgaacaca ttccgtcata
     2581 agatgctgaa tcaattccat ttaaaagagt ttgaattatc acaaaattac acttttttct
     2641 gggataaata tgaaaaggca aattatttct atgaaaacat cattgcaaca ggaaaagagg
     2701 caatcgatag ccggaaagta gctttcttac tcgctactcc tcaacaagat ggcggccaat
     2761 gggatatgat cgtttcatta ttccaaaaat atggcgttgt gcctaaatcg gtgatgccgg
     2821 aaagtgcgaa tagttcaaat tctcgagatt tgaataacta cttgaacaaa ttactgcgta
     2881 aagatgcagt agtgttacgg aaaatggttg ctcaaggaga aacattagac gctattgaag
     2941 aaaagaaaga agaaatgcta gaaagcattt ataacttctt agctatctca ttaggaacac
     3001 ctccaaaaga attcgatttt gagtaccgcg atgaagaaaa gaactatcat cttgaccgag
     3061 gactgacacc acaaattttt tatgataaat acatcggtgt aaaattagat gattatgtta
     3121 gcgtgatcaa tgcaccaaca aaagacaaac cattcaatcg ttcttataca gtagagatgc
     3181 taggaaatgt cgttggcggg aaagaagtca aatatctgaa tgttgatatg gaaactttca
     3241 aaaaattagc gattgctcag cttgaagaag gagaatctgt ttggtttggt tgtgatgtag
     3301 gtcaatcttc tactagaact agtggcatca tggccttaga tgcttattca atggatgatc
     3361 tatttgatac ggattttaca atgactaaag ctgaacgttt agattttgga gaaagtctga
     3421 tgacccatgc gatggtattg accggtgttg acttagtaga cgggcaatca acaaaatgga
     3481 aagtcgaaaa tagctggggc gaaaaagttg gaaagaatgg cttctttgtt atgagtgatg
     3541 cctggatgga cgaatatacg taccaaatcg ttgtaagaaa agatttattg acgaaagagc
     3601 aattaaatgc gttcaatgaa gaaccaattg ttctatctcc gtgggatcca atgggtgctt
     3661 tagcatagac gagtatttca atgaagagta tgtagtaaag ataaaaagag ctggcttaag
     3721 ccagctcttt ttatctttaa agtccattcc ctgtttttta tggtccctgt gctataattc
     3781 taaataaagg ataaggattg gaaataatcc ttttatcatt aaaaatatga tgacgaagta
     3841 gaataaaatt gggagtaggg gggaaagtca tggatatcca aactgtttta tctgatctga
     3901 aattaaatgg taaagtgatt ccagttgttg gtggcgatgt taatcagacc tatcgtataa
     3961 aaaccgaaca tcgtgcttat tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg
     4021 aagcagaagt agacggatta aaagaactgt cagcattcgt acgtgtaccg gatacgtata
     4081 tgctaggtga aacaagtgaa ggcgcttatt tgttaatgga atggatcgag ccgggaaaag
     4141 gggatcaacg agatcttgct gcagctttag ctaatctcca tcagcaaaca gcgccacaat
     4201 ttggttttcg taaagataac tatttgggta cacttgttca gaaaaattct tttgaagaag
     4261 attggtggac ttttttcttt aaagatcggc ttgaatccca gatttctttg gcagaagaaa
     4321 cgaatcgatg gaatgtacag cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga
     4381 aatcggttga accaaaaaag atcactccac gcctcttgca cggagacctg tggagtggta
     4441 atgtgttctt tgaccagcaa gggcatccag tttttgtcga cccagctgtt tcttatggga
     4501 atcgggaaca agatattgca atgagccagt tattcggtgg tttccgaccg gaatttttag
     4561 atgcttatca aactatattt ccattagaaa agggatggaa agaccgactg ccgatttacc
     4621 agttatatta tcttttggca catttgaata tgtttggaga aagttacggt tcccaagtag
     4681 atcaattact agaaaatttt taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa
     4741 agcatgaagt ttcatattat gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt
     4801 taggagtagt tatcaaaacg tctgaagatc aaagcgacca gttgggaaga ggcagtgaat
     4861 tcgtcaatag ctttggactt acttgggtca tcacgaatta ttccatggag attaatcgtc
     4921 tccctagagt cggtgaagta atctcagtaa cgactcaagc catggagtac aataagtttt
     4981 tctgttatcg gaacttctgg atccatgatg aagatgggaa agaattagtc aaaatagaat
     5041 ctgttttcgt attgatggat tttgtgaatc gaaaaatgag cagtgttaat gaagagatca
     5101 ttgctccttt tgagagtgag aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga
     5161 ttgattcggg cttcatgttg ccatatcgtg ttcgttttta tgatatcgat agtaatcagc
     5221 atgttaataa tgcgatgtat tttaattgga tcattgacgt attaggatat gactttttga
     5281 ctacgcatgt tccagaaaaa gtaattattc gatttgataa agaagtagag tacgggaatg
     5341 agatcgaaag ccattttgaa caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa
     5401 tccgaatgca agaacagctg tattgtgaag caaatattat atggaaaaat aaataaaaaa
     5461 gaggttgtga cagaaggggg caacttcaag aaataagaag aaatttacga aaattgtttc
     5521 tcaaattttt gtaaattttc gacttagttt tcgaagaagc tatttttgtc acagcctctc
     5581 tttttaagtt tagctattag ataatgatgt ttagttttgg ataaacgaaa tcaattcttc
     5641 acccgacaaa ccatagttta cagcaatcaa tagttttaaa cgtgcttttt gactattaat
     5701 actgttacag aaaataatcc ccattttttc tagttctttt cccccgcctt cataatcata
     5761 aacaggctct gcgattccat tgaaacaacg agaaacaagt actacaggga ttttttggac
     5821 aagaagcttt tccaaagcag ctaaggcttg tggagggaga ttccccgcac ctaaagcttc
     5881 gattaccaaa ccgtctactt tcgtatgggc gatggcttcc aataagtccc cttgcatccc
     5941 agcatatgct ttaacgatcg ggatagtccc atctacagcg gaaatgtcca gtcgctttgt
     6001 ttctaaaagt tcctgcaaga ataaaattcg attcttagtt actaagccga tgggacccaa
     6061 tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga
     6121 atggatctca tcgttcatga ccaccaaaac ccccttatct agcgcttctt cacaacttgc
     6181 gacacgaatc gcactttcaa aattataaag gccgtcactt ccaagttcat tacttgaacg
     6241 cattgcccca gtcaaaacaa ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc
     6301 agtttcttct aatgtatctg taccgtgtgt gatcaccact ccggatatac ctgatgcata
     6361 agcggacaag atacgttctt ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg
     6421 aagattgaag atatcttcaa ctaataattc tacatgcttg gggattgtga tttctgcatc
     6481 taataatggg tttgccacat caggactaac accgcccgtg agcatgtttt ctttcatggc
     6541 gatcgttccg ccggtgtgta gtacaagtat ttttttcatt ttacagactt ccttacgaat
     6601 aattttgcat agccgttaaa tcttattgta tactatataa aagagttttg ctatcagtga
     6661 agaataacga atagcctaac ttatcataaa aaagctgacc ttattaaaat ggaaaagact
     6721 caaccttgac ctcctaatac aatggagtat ctggttgagt ttcttttaca agaaagcagc
     6781 caaggaaatc aatcacgaag gtggccggaa atgatcagaa tcggattaac atcgtttaaa
     6841 gaacatgaca aattaactgg taaaaagaat aacactcttt atgaatatgc agggtttcta
     6901 cccttagtgg aattagatac tgcctattat ggaattcccc gaaagtcctc agttgagaat
     6961 tggataagtg aagtacctga atcatttcgc tttgtagtca aagtctatca aggaatcagc
     7021 ggtcaaggag actggaagcc ttattatcag accgaagaag agatgattgc gcatttttta
     7081 gatgttatgt cgccaatggt agatgctgga aagatgtttt gctttttagt ccaattcccc
     7141 tcatcattca aatgtacgaa agaaaatgtc cagtacttgc gcaaacttag aaagtggttt
     7201 gagggttatc ctgtttctat agaattgcgg gattattctt ggtacagcga tgaatttctt
     7261 gaacgtacaa gaaactttat gtctagagcc gctttttctt tagccatcat cgatgagcca
     7321 cagttgctga atacaacgat tccttttgat ccatttgtca ccaatccgaa ctttgctctt
     7381 tttcgattcc atggtaggaa tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa
     7441 aaaagaacgt tatatcgtta ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa
     7501 gtagttgatt ctaagacaaa agaggtaggt gtgattttta ataataattc tggtggtgat
     7561 gcggctgaaa atgcattagc actgcaaaaa atattaaata tcaagccaga tggactcaat
     7621 ccaacccaaa tggatctgtt ttaagggctt attttattca agaaaggggc gaagtctatg
     7681 atcaaagcta ttttttttga tattgacgga acattggtca ccaatcgttc aaaagcgttg
     7741 gaatcaacaa aacaagcaat tgaatatgct cggaaaaacg gcattttatg cggtgtagca
     7801 acggggcgca gcccagtaaa gatcaaagaa atcattgatg aattggaatt agatatgtat
     7861 gtcgtgtata atggtcaact ggtttttact gcagatcgca cgattatcga ccaccctttt
     7921 gaacaaaaag tattggaaca tattgttgaa tttgcagatg agaaccatcg acaaatcgtt
     7981 tttggtgcga gaaatcggtt agatggatca acgacgatgc tattaggaca atcgattttt
     8041 atcaagcgac tagtcagctt tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc
     8101 ctccaggttt ttagccctca ccgacaaaag gatcgttatg aaacgttaga gatattaaaa
     8161 gaaccaattt atcaatgtat attgctcagt ccggagtcag aacagaaaaa gttagaacat
     8221 cgctttcccg agtgtacgtt tcagcgttca aatacgtata ctgtagatat catcccaaaa
     8281 ggtggttcaa aattactagg catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa
     8341 gaaattatgg cattcggcga tcattacaat gatatcgaga tgctgaaagg tgtaggaatt
     8401 ggagtggcca tggggaatgc tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa
     8461 tcaaatgaag aagatgggat atacgaagga ctgaaacact tcggtctgat ttattagtca
     8521 atgaaagaag gaaaaaatca gtatggaagc agagacacct tttcaaaaag tagaaacatt
     8581 ccacaaaact tttgatccaa gaagaccaga gatacccacg gcttttacag aaaatgaagc
     8641 tcatacacgt gctgcgttca aaatagaaga acttgttgaa tttgttgcag ctacttcacc
     8701 taataaagaa caactgcatg aagctgttat gctgctgcac gaagcattag ataaagcaga
     8761 agaaaaaata cttggtaaaa cacagtgggg aagtgatccg ctggttgaac aagtagatgc
     8821 actgacagat atcctttact ttacttatgg ttctttttca ttactaggaa tagtgcctga
     8881 tcgaatattc gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag atggaaaacc
     8941 tcattatgac cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga tggaatatgc
     9001 tccagaacct aaaatcgcgg cagaaatcca acgtcagatt caagaagcga ttgctaagaa
     9061 gaaatccgct gatataggca tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt
     9121 ttgttatctt tatacgatgg aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa
     9181 aatagtatgc tataatctag aatagatgtc ttttgcacga gagacgtatt atttggattt
     9241 atgccaagca gaacgccatc tgttgcataa aagatgcata ctgaaaacgg aggaaccata
     9301 aatgggaaat aatgttatca tcggaatcgt cattgcaatc attatagtcg ccattgtcgt
     9361 ctttttggtt agttacttga tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa
     9421 acgcaaggaa gaattatttg acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat
     9481 gcatctggtt gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat
     9541 atctacttcc tcgttcgctg aactagaaag tcaaattttc gaagtggaaa acctcaatga
     9601 aagttttcgg ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta caatggacca
     9661 aatggagaaa caagtgacgg aaatccgtga aggtctaaag gaattgcggg aaacggaaga
     9721 acgcaactca ctagctgttc agcaggcact tgacgtctat gaagaactga aagaaaaagt
     9781 cagagaagac gagcatgaat acggaccagc tctttttgaa ttgcaaaaac agatcaaagt
     9841 gatcgaaaaa gaatttacgc agtttgtgac tttaaacact tctggagacc ctgttgaagc
     9901 ccgcgaggtt ttggataaag cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa
     9961 aattccaccg ctgtatgagg agctccatac cactttccct gaacaacttg aagaaatcag
    10021 tgatacttac gagaaattga tggaagaaca atatgtattc ccagaagaag atcttgctga
    10081 agatatcgca aaagtaagtc gaagaattga aaattcatta gccaatcttg aaaaaacaga
    10141 agttgaaacg gtcgaatttg aaaaccgtga aacagctgat ttgattgatt cgttatatga
    10201 tatcttagaa cgggaaatgg aagctcagag atatgtaaaa actaaccaat caacgattgc
    10261 agagtatatc aaacatacaa caaagaataa ccgtcaatta ttgattgagc tggatcacac
    10321 atctcaaagc tatacattga accacaatga aattggccgt gtacgaggtt tccaaaccga
    10381 agtggaagaa atggaacgtc aaaatgaaca gatgattcca cagatccgtc aacacgaaat
    10441 tccttattca gaagtgcgca cgttctataa aacagtcttc aaagtcttag aagatatcga
    10501 aaagcaacaa gtagagatcg acgactcatt acatgaattg agaaaaggcg aaaaagaagc
    10561 gcaagaaaag atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa
    10621 acaacgtttg cctggcttgc cgaatgatta cctagaattc ttttttgttg ctactgaccg
    10681 tattgaagaa ctaagtgttg tcttgaataa aattcgagta aacatggaag aggtcaatcg
    10741 tttagttgct ctatgtgaag aagatctaga attattagat aagaagacac atgatttagt
    10801 agatgcagca gcattaacgg agcaaatgct ccaatatgca aatcgttatc gtcatacaca
    10861 cgaagaagtg agagaagcaa ttgataaaag ctactattta ttcaataaag aatatcacta
    10921 tcaagaagca ttagacgaaa tcggtacagc attagagcga gtagaacctg gtgctttcaa
    10981 acgcatcgaa gatttttatt ttaatcatcc agacttagta taaagcaaaa aagttagtaa
    11041 accagcaaga gcaagacgga ttattgtctt gttcttgtct ttttttgtat atttgaaaat
    11101 tgaaacaagt gaaaatttca cctgctcttg aaagcacctt ggacaaccgt tataatagaa
    11161 aagaatttca aaaaagaggg gtttcttatg atatattttg ataacagtgc aacaacccca
    11221 atctttccgc aaagcttaga tgcatatgta aaaaccagtc agcggattat tgggaatcca
    11281 tcaagcttgc atgatttagg caatcaagct aaccggttgt tgcagcaagc aagaaagcag
    11341 atcgctgatt tgatccatgt agaaccagaa gaaatctatt ttacttcagg cggaacagaa
    11401 ggagataact gggtattaaa aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg
    11461 atcatttcag gagtcgaaca tccggccgtc agcgagacag cagaacaatt aaaagaatta
    11521 ggaatcgaag taagtatcgc cccagtggat aaacgcggat ttgtacgtgt cgacgaattg
    11581 aaagaattaa taagaaaaga gacagtatta gtctctgtta tggctgtgaa taatgaagta
    11641 ggatcgattc agcccatcca ggaaatcagt gatcttttgg atgattttcc taaaattcat
    11701 ttccatgtag atgctgttca ggcgattgga aaagtaccat tctcgcaatg gttgacagaa
    11761 agagttgatt ttgcaacatt ttcagctcat aaattccatg gcccacgggg aactggtttc
    11821 atttattgga aaaagggtcg tcgtttagca ccattactga caggtggagg gcaagaaaga
    11881 aatcagcgaa gtgggacaga aaatgttcca ggtattgtgg ctatggttaa agctttacgt
    11941 ttatcattag agataaagga acaaaaacct gagcagacac aacgcttaaa agtagctcta
    12001 atcaatgcgt tgaagacgta tgagaaagtg acgatttttt cagaaggaac tgactatgca
    12061 ccgcatattt tatgttttgc tctaaaagga attcgcggcg aagttctcgt ccatgcctta
    12121 gaagaaaaac aaatatttgt ttctactact agtgcctgct cctcacgtaa acatatggca
    12181 agcagtacat tgcacgcaat gcatgtacca aatgatctgg ctacatctgc tgttcgaatc
    12241 agcttagatg aaaacaattc gcttgcagaa gtcgagcaat ttatgattat tttcaagcag
    12301 ctttataaga aattttctgc aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa
    12361 attatggtcc gttatgggga actttcgaca aaaggaaaga atcgaaaaag ctttatcatg
    12421 caactagcac aaaatgtcag acaagctttg aaagactttc cagaaatcaa aatccatgct
    12481 gatcgggacc ggatgcatct tttattgaat ggcgcagacg ggcagctagt tattccaaaa
    12541 ctagcaaaaa tatttgggat ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc
    12601 gaagttttga aacaagctgt tcaagacatc atgaaagaaa tttatactgg gaaagaaaca
    12661 tttaaaatta tcgctaaaag aagcgatcac caatttgaat tgacaagtaa tgaattgaat
    12721 caaacattag gtaatgctgt ttttgatatt ttcccgcata tccaagttca aatgcgtcaa
    12781 ccagatattc cattacgtgt agagattcgg cgtgatggcg cttatctatc gtatgaaacg
    12841 atcaagggag ctggaggatt gcccgtagga acaagcggaa aaggaatgct tatgctttct
    12901 ggggggattg attctcctgt agcagggtac tttgccatga agcggggagt ggaaattgaa
    12961 gctgttcatt ttgctagtcc gccgtataca agtgaacaag cactgcaaaa agcaaaagat
    13021 ctaacagcca aactagcacc ttatgtggga acgatccatt ttatcgaagt gccatttacc
    13081 gagattcaag aagaaatcaa aaagtcagtg ccacaaggat attggatgac aattactcgt
    13141 cgaatgatgt tgcgtttgac cgatgaaatt cgccgtattc gtcatggttt agtcattttg
    13201 aatggtgaat cattaggtca agtggcttca caaactttgc aaagtatggt ggctatcaat
    13261 gaagtaacga atacgccaat cattcggcct gtagcgacaa tggataaact tgaaatcatt
    13321 gaagtagcag aacagatcga tacatttaat ctttctatcc agccttttga agattgctgt
    13381 acgatttttg ctcctccaca gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat
    13441 gaagagcgtt tagcaataga tgaaatgatc caacgagcac tagctggctt gaagatggaa
    13501 gagatacaag cagaatcgtc taatgaaaaa actgaagaat tttcagaatt gctataagag
    13561 gttatgaaaa tagtgttttg accagagtat tagagaaaca aaacgaaaaa atagctccat
    13621 gtattttacc gtttttgaac taatgtcgaa gattagctga ttgaatacca tttatcaaga
    13681 agttatggaa aagccggttc ttttttacta actgaaaaat aaaattttat ttagagatca
    13741 agctacagtt gataaatgat ataaaaaaga gaatttccca tgaaatagcg aggttgtgac
    13801 agaagtgggc agcttcaaga aataagaagg aatttacgaa aattgctttt caaatttttg
    13861 tgaaatttcg gcttattttt cgaagaagtt acttctggaa caccgtgtat cagaggttgc
    13921 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt
    13981 tacgtttggt tgagcttatt accgcagatg caagcttttg aacaccgttt tatttgtctc
    14041 acaccctttt ccttcctact tgtaaccttg ctcccgtgtt agaaacatgt tacactaaat
    14101 atgtgaaatt attaacaagg agcgtcgtta tgcgagaaaa aacagataca ctgccaaaag
    14161 caactgcgaa aagattacct ttatatttac gctacttgaa gatgctggat gactcaggta
    14221 taagtagaat caaatcaaat gagttcagcg aaatcaccca aattccttct gcaacgatcc
    14281 gcagagactt ctcgcaatta ggcgagctag gcagaagcgg atatggctat gatgtccctt
    14341 ttcttatcga tgtattcaat aatatcctta atactaaaga agaaaaacga atcgcattag
    14401 taggatatgg aaatcttggc aaagcattga aacacaacaa ttttagaaga aatgaaaatc
    14461 taaatatcgt gtgcgtcttt gataatgatc cagcactgat caatcgagta attgatggag
    14521 aaatgatcta tccgattgat cgtttcgcgg agatagccaa agcaaaaaat gtgacagtag
    14581 caatctctac ggtaccaagt aaatattcac aatctgctat tgatgaaatt gtcaaaggaa
    14641 atgtaaccgc tatacttaat tttgcgcctg acagagtaac tgtcccagct tatgtcaatg
    14701 ttcaatacat tgatttgaca accgagttgc agacgcttat ttactttgat gagaattact
    14761 cagaagtttt ttcttaggaa aatttgagta ggcttattga tcttgcgaga actcgtcgta
    14821 attctttcaa aaatttttta gtactaaatg aagtggtttt ttcacgacca cttgttgact
    14881 aaaaaaaaaa accattgtag aatgaagcat aacgctgaac aagaggagta actggcacat
    14941 tcattcttag agagagaact atctgctgaa aggttctcaa tgacaaccag tgaaggtagc
    15001 ttgggagttt ctgtcttgaa tggagtagag atagacgagt cgtgatcgtt aactcacttg
    15061 agaggcaata atgattgatt attgcaattt aggtggtacc gcgtaaatca tacgtcctag
    15121 aatacgaaag tattttagga cgttttttag tttaaaggag gaaaacagca tgacggaaga
    15181 aaataaccta tcaacaaaat ataatccgca agaagttgaa gctggacgtt atcagcattg
    15241 gttagaacaa gatttgttta aaccgagcgg agataaaaaa gcaaaacctt attcaatcgt
    15301 aatcccgccc ccaaatgtta ctggaaaact tcatttaggg catgcttggg atacgacctt
    15361 gcaagatatg ctgattcgtc aaaagagaat gcaaggatat gatactttat ggctgcctgg
    15421 tatggaccat gcaggaatcg cgacacaagc aaaagtagaa gcgaagctta gagagcaagg
    15481 gatttctcgt tatgatctag gtcgtgaaaa gttcatcgat caagtgtggg aatggaaaga
    15541 agagtatgcg agccacatcc gcgaacagtg ggctaaaatg ggcttatctt tagattacag
    15601 tagggaacgt tttacattag atgatggatt atctgaagca gtacgtaaag tttttgttac
    15661 cttatacgaa aaaggtttga tttatcgtgg tgaatatatc atcaactggg atcctcaagc
    15721 acgaacagct ctatctgata tcgaagttat tcataaagat atcgaaggcg ccttttatca
    15781 tatgagctat gaactagcag atggatcagg cgtagtagaa attgcgacaa cccgtccaga
    15841 aacgatgttg ggagatacgg cgattgctgt tcatccagaa gacgagcgtt atcaagcgct
    15901 catcggcaaa aaagtgattc tacctttagt caataaacaa atcccaatca ttgcagatga
    15961 atatgtagat atggaattcg ggacaggtgt agtaaaaatc actccagcac atgatccaaa
    16021 cgactttgag gtaggtaatc gtcatgatct tccacgcgta aatgttatgg atgatgatgg
    16081 tacgatgaat gaactagcag gaaaatatgc tggaatggaa cgttttgctg ctcgtaaagc
    16141 aatcgtgcaa gatctgaaag aaatcggtcg attgattaaa atcgatacga tgatccacag
    16201 tgtgggtcat tccgagcgga caggtgtcgt tgttgaacct cggctgtcta ctcaatggtt
    16261 tgtaaaaatg gcgccgttag cgaaaaaagc cattgataac caagaaacag atcaagcagt
    16321 ggaattcttc cctccaagat tcaatcaaac gtttttacgt tggatggaaa atgtccatga
    16381 ttgggtaatc tcacgacaat tatggtgggg acatcaaatt cctgcatggt atcacaaaga
    16441 aacaggtgaa atgtatgtag ggatggaagc accagcggat agtgaaaact gggtacaaga
    16501 tcctgatgtt ttagacactt ggtttagttc agctttgtgg cctttctcaa caatgggctg
    16561 gccagatacg gaaagcgaag attataagcg atacttccct actagcactt tagtaaccgg
    16621 ttatgacatc attttcttct gggtaagcag aatgattttc caaagcttgg aatttactgg
    16681 cgaacgtcca tttgagaatg tcttgattca tggattgatt cgtgacgaac aagggcggaa
    16741 gatgagtaaa tcattaggta atggaatcga tccgatggaa gtaatcgaaa agtatggagc
    16801 agatgctctc cgctggttct tatcgaatgg ttctgcacca ggacaagatg tccgcttcag
    16861 ctatgaaaaa atggatgctt cttggaactt tatcaacaaa atctggaatg cttcacgttt
    16921 cgttatcatg aatatcgaag gaatgaccgt agaagagatc gatttatctg gagaaaaatc
    16981 agtggctgac cggtggatcc ttactcgttt gaatgaaacg atcgaacgag taactgagct
    17041 atttgatcgt tttgaatttg gtgaagcagg tcgacaactg tataacttta tctgggatga
    17101 tttttgtgac tggtacatcg aaatgagtaa agaagtactt tatggagatg atgctgtttc
    17161 aaaacaaacc acccgtagca tcttagtcca tacacttgat cagatcctac gtttattgca
    17221 tccaatcatg ccattcgtta cagaagaaat atgggaacat attcctcatc agggaaattc
    17281 tctagtagtg gcagaatatc ctgttgtacg tccagagttt gatgatgaaa cagcttctaa
    17341 agggatggaa gtattgaaag aattgattcg agccgttcga aatattcgtt cagaagtgaa
    17401 tacaccattg tctaaaccaa ttacgttgct aattaaaaca aacgatgcta aaatcgatca
    17461 atttttagtt gaaaatacaa gctatatcga acgcttctgt aatcccgaag agttgacgat
    17521 atcaagtgag atcactgcgc cagatctagc gatgtcagct gttctgacag gtgcagaaat
    17581 cttcttgccg ttagcaggat tgatcaatat tgaagaagaa atcaaacgat tagaaaaaga
    17641 actggaaaag tggacaagcg aagtaaaacg tgtccaagga aaactttcta atgaacgttt
    17701 tgtttctaac gcgccggaag aagtagtaga ggcagagcgt gcaaaagaaa aagattattt
    17761 agagaagcaa acggctgtca aagaacgaat cgaaagtttg cgtactattc aataagaata
    17821 gaaaaagaga gtgtgaaatc agctagctat tactaaaagc tagctgattg aacatcgtta
    17881 caggaaagag tttgagcctc actcttagct tcgattcata aggagatgtg acataaattt
    17941 tgtcgcatct cttttttagt ctactcttat tatgtaggag aaggagggag gtggatcatg
    18001 tatcaagagt caataaagta gttgataaaa tcgttcctct ctctgtttct tttctctttg
    18061 tcatactttt atttttctta tgtgttctat ttgttataat aaaagagaaa tgggggaatt
    18121 gttgatgacg attgaagaag caatagaatg gatccatagt cgattaccat ttggttcacg
    18181 tcctggcctt gaccgagtcg aagctttatt agaagtggta gggcatccag agagaaaagt
    18241 aaaaacgatc catatcgctg gaacaaacgg aaaaggttct actgtagctt atttgagaag
    18301 cctattagaa gaggctgggc tgacagtagg tacatttact tcaccttata tcgaaacgtt
    18361 caatgaacgg atcgcaatca atggcaaaag cattccagac aatgatttga tccagcttgt
    18421 aaaaaaatat cagccactga tcaaacaact tgatcaattt gaagcagtag ctggtattac
    18481 tgaattcgag acattaacag caatggcatt tgattatttt ttgatgaaga aagtggacgt
    18541 agcgattatc gaagttggct taggcggctt attggattct accaatgtag ctgtaccaat
    18601 gctgacggga ataacaacga ttggacttga tcataccgaa atacttggag atacaattga
    18661 agaaatcgct gcacaaaaag ctggaatcat caaacaaggc gttccagtag taacaggaaa
    18721 tattgcccca gaagctttta ctgtcatcca acaaacagct aaagcaaaaa atgcaccgct
    18781 ttatgtatac ggggaaaatt ataaaataaa ctatctgcat cctgatgaga tgtggggaga
    18841 agtatttgaa ttttttaatg aaataggaaa gatccctcaa ttaaccactc cgcttttagg
    18901 gcaacatcaa gctgaaaatg caggagtagc catcgaattg tattttgttt actgtcaatt
    18961 ggaaaagctg cctttccgag aaaaagatgt tttgaaggga ttgaagaaat ctcgatggcc
    19021 aggaagaatg gagcgtttga gtaacgagcc attagttgtt ttagatggtg cccataatac
    19081 gcatgcaatg aaccggcttg ttgagaattt gaaaaaagaa ttcaaaggat atcaaatcaa
    19141 cattttgttt tccgccttac gaactaaaaa tgttagagaa atgctagaac gattgcagca
    19201 agtaccaaat gtccatattt atttgacgac ctttgaacat ccaaaagcca tcgaattaag
    19261 tgaagtatca gatttagcaa gtgagagagt atcaatcgtt tcattgtggc aattcggatt
    19321 agcagatata ttggaaaaga tgacaaatga agacgtatta gttgtgaccg gttcacttta
    19381 tttcatttca gaagttcgcc atttattaat acaattagga ggagatcatg aagtataatg
    19441 cagtgatttt tgacatggat ggtttgctgt tcgacacaga gttagtgtat tacgaagcca
    19501 cacaagtagt agcagacaag atgggttttc cttatgataa agacctgtat ttaaaattct
    19561 taggtgtttc agatgaagaa gtatgggcga attatcatat gattttctcg gaatttggca
    19621 aaacaagtgt acaaagattc atagatgatt cataccagga aacgcttgaa cgtttttcgc
    19681 aaggagcggt acaaatgaag cccggtgtgt tggaactcct ctctttttta gaagaacatc
    19741 agattcccaa agttgtcgct tccagtaacc aacgagcagt cattgaactt ttattgaaga
    19801 agaacaatct gtttgaacga tttgagacga ttgtttctgc agaaaatgtt caacgagcaa
    19861 aaccagaccc agaaattttc ttgttagcta atcagaagct aggtacagcg aagaaagaaa
    19921 cattgatttt agaagattca caaaatggta ttttagcagc ttctgcggct gagatcccgg
    19981 tagtcatggt accagatctg cttccaccat cccaagaatt gatcaaaaaa acagctgggg
    20041 ttgtttcttc tttacatgaa atccctgcat tgttaaaata aaaatccatc tcttttcgta
    20101 tcttatttta gataagatag aaaggagatg tttttttggg aaaacgattg atcaaagaag
    20161 tacctactag ctctttacca agggaacgta tggaaattta tggcgcggaa gctttatcgg
    20221 atcaggagct tttggcaatc ttactgcgta ccggtcaaca cccttatagt gtgatgtcga
    20281 ttgctggaaa tctgttaaaa acgtttggag gactggcctc tttacgacag gctactttgc
    20341 atgagttaga agagattcga gggataggac gcgtgaaagc aatcgaaatc aaagccttga
    20401 tcgaattagg tcgacgaatt cagacggatc atgaaagagc ggctccaccg gttcgtacca
    20461 gctacgaatt ggcccagcaa ttgattctgg aaatgaaaga tcacaaacaa gaacatttgg
    20521 tttgtattta tttggacacg aagaatcaag tgatcttaaa aaaacagtgt tcatcggttc
    20581 gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac gttattgtgc
    20641 agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat cgcagcagga
    20701 tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca ctgtactgga
    20761 ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa tgatggaaga
    20821 gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat tgtaattttg
    20881 tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca atgttacaat
    20941 ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac agtaaaatgg
    21001 ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga cgtattcgtt
    21061 cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca agcagtgact
    21121 ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa agcttaattt
    21181 tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa gcgagtaaca
    21241 aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt atgaaaaagt
    21301 aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga acaaacaaaa
    21361 atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg ctgatttcat
    21421 aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt ggatgtcttt
    21481 ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc aatttatatt
    21541 ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt cgctgagctt
    21601 cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga atatctggac
    21661 cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca agaccttctt
    21721 ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc ccgctaccat
    21781 cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg
    21841 ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct acatcttgtg
    21901 ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata aaatattttg
    21961 gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg gaagtatgga
    22021 tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa ttgatcaaat
    22081 gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact gtatcgtctg
    22141 gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt
    22201 ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga ttgatttcgt
    22261 gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga ccgttgacga
    22321 tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc acagcggcat
    22381 ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg atacgtcctt
    22441 cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat ttagtaaatg
    22501 ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct tccccgatac
    22561 gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat ttgaagtctc
    22621 cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca tctgggatac
    22681 tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc gtatcttcat
    22741 tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg tttgtgatca
    22801 acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc aagtagggta
    22861 ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt
    22921 tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa tcgtcttccg
    22981 ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc ccatacgtat
    23041 ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta tttttgatgg
    23101 aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc gtactctaga
    23161 tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc ttccacacgt
    23221 tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc ttcccgttta
    23281 gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt gtctccttta
    23341 tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa cgaggggaca
    23401 agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa agagacaagc
    23461 ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat aaaaaatata
    23521 caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc aactagattg
    23581 aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt atgagagaag
    23641 tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta acaaaagatt
    23701 aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata agaaattctt
    23761 gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac ttcctacgat
    23821 tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg tgaagcaaca
    23881 agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct tgttaggtta
    23941 ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa
    24001 gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat tgatcactcg
    24061 tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac gtatcggttt
    24121 tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact
    24181 cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag cgattgctaa
    24241 ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca acagtatctt
    24301 gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga
    24361 aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct tattccgttt
    24421 gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg tattgatcat
    24481 ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt
    24541 tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg tgttcatgat
    24601 gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg ccaaaccttc
    24661 gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata tcgaatttca
    24721 aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa atgtcagttt
    24781 tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg gaaaatctac
    24841 gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg
    24901 agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt ttattccgca
    24961 aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga aagaagatgc
    25021 tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat tcatttctcg
    25081 aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt caggtggaca
    25141 aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct atatttttga
    25201 tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa
    25261 agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga tcatgcatgc
    25321 agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc atcgggagtt
    25381 gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa aggaggaatt
    25441 agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag accgtatcgt
    25501 gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa cgtggctttg
    25561 ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa tggcgaatca
    25621 atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg aatagggtat
    25681 tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag tgccatgcat
    25741 gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata tttcgataaa
    25801 aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct
    25861 ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt ggctgcagca
    25921 atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat tccaacatcg
    25981 atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag catgcagaat
    26041 tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag tgtattgaaa
    26101 gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca cagcttgaaa
    26161 tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt tcaattaact
    26221 gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat tactggggcg
    26281 atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag tcagccaatg
    26341 gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac gaaacgtgta
    26401 ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc actgccagaa
    26461 acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc gaagaaacca
    26521 ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg
    26581 accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga cgtagacaaa
    26641 ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg
    26701 ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc agataatatc
    26761 cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa aacagcaaac
    26821 gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa ttctgaggga
    26881 gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt ggtctccgat
    26941 cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt agaagcgttg
    27001 attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt
    27061 ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca gatcattgaa
    27121 aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact gtataacagc
    27181 caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa acttattttt
    27241 tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg acacggagat
    27301 ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc gtttaacgca
    27361 cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt ttcaaatcga
    27421 acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa tagaagaaaa
    27481 tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa atagacatct
    27541 ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct atgatacctt
    27601 tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat ggaacggttt
    27661 atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt gacgatctgg
    27721 tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag aacgaatatc
    27781 caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc ggagaatgtg
    27841 acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc gctgatcggg
    27901 atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat tggtgctgga
    27961 tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg tcgcccagct
    28021 aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa catccagcat
    28081 tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag ataggcagta
    28141 gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt tatttggttt
    28201 agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa gaactgtttt
    28261 ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata tagttgtcac
    28321 gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct aaaattttgt
    28381 ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc
    28441 cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa gcaaatcgtt
    28501 taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata cccataacaa
    28561 ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata cctgctgtaa
    28621 ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct tgagcgtcta
    28681 agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg ccgcttaaca
    28741 tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca cctcgtgaag
    28801 attgttcaat gactgtagga attaaattca taatagagtt cctccttgat ttcgtttaga
    28861 aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt ttggtcaaaa
    28921 aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg gtatgataag
    28981 cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg ccttatcgga
    29041 gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa
    29101 aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga gacgctgaat
    29161 caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat ggaagaatat
    29221 gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc gtatctccaa
    29281 tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga gctatttaag
    29341 aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat tggtggatat
    29401 gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac tgaaatgatc
    29461 aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt cttctcgaaa
    29521 gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc gtggtaatgc
    29581 tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat gttacaaatc
    29641 tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc atttgcagaa
    29701 gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa agatggttat
    29761 ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg gctgattcga
    29821 gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag caaagaattt
    29881 ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca aaaaaattac
    29941 cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg tatcgaagaa
    30001 aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata ttgcagcttt
    30061 catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct ggacttattg
    30121 atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat agaaggagta
    30181 catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt tccaaaagca
    30241 attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga actcaaaata
    30301 gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt
    30361 aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg taaaacagcg
    30421 ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga ttcattgcaa
    30481 atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat gaataatgtt
    30541 ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc tgattttcag
    30601 cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc tatcatagct
    30661 ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg agcgacagaa
    30721 gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa
    30781 gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat ccactggaac
    30841 aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca tagtatcacg
    30901 gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt agatacgaag
    30961 cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc agaaggttta
    31021 gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca aggtattgga
    31081 tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc aacagaacaa
    31141 gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg
    31201 gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca
    31261 atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga
    31321 aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc agctgacgaa
    31381 taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag tggatcggca
    31441 aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg cgcatgaagc
    31501 ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga ttaccgatgc
    31561 tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg cgatgcgtgc
    31621 tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc tgctgcctcg
    31681 tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa caagagggcc
    31741 aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt ttgctgttaa
    31801 gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag
    31861 cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa
    31921 tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt tagatccatt
    31981 gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata ccgtcgggtt
    32041 catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag aagaaagtca
    32101 aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta ttttacagga
    32161 gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga tactgactgt
    32221 ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat tcccaaatgt
    32281 attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca
    32341 gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag gacagaaatt
    32401 aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag aaacaaacga
    32461 atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa
    32521 ttttattttt ttaa
//
LOCUS       DANKMN010000031        31896 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000031 DANKMN010000000
VERSION     DANKMN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(26..601)
                     /locus_tag="PW224_001321"
     CDS             complement(26..601)
                     /locus_tag="PW224_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2908020.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="PW224_001322"
     CDS             complement(606..1580)
                     /locus_tag="PW224_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HDL2908021.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="PW224_001323"
     CDS             1863..2831
                     /locus_tag="PW224_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2908022.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="PW224_001324"
     CDS             2928..3221
                     /locus_tag="PW224_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2908023.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="PW224_001325"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="PW224_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL2908024.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="PW224_001326"
     CDS             3678..4433
                     /locus_tag="PW224_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2908025.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="PW224_001327"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="PW224_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL2908026.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="PW224_001328"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="PW224_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL2908027.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="PW224_001329"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="PW224_001329"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL2908028.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="PW224_001330"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="PW224_001330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL2908029.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="PW224_001331"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="PW224_001331"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL2908030.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="PW224_001332"
     CDS             9170..9712
                     /locus_tag="PW224_001332"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL2908031.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /locus_tag="PW224_001333"
     CDS             9735..11291
                     /locus_tag="PW224_001333"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL2908032.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="PW224_001334"
     CDS             11305..12207
                     /locus_tag="PW224_001334"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL2908033.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="PW224_001335"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="PW224_001335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL2908034.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="PW224_001336"
     CDS             13650..14072
                     /locus_tag="PW224_001336"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL2908035.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="PW224_001337"
     CDS             14541..14771
                     /locus_tag="PW224_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL2908036.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="PW224_001338"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="PW224_001338"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2908037.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="PW224_001339"
     CDS             16182..16358
                     /locus_tag="PW224_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2908038.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="PW224_001340"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="PW224_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL2908039.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="PW224_001341"
     CDS             16853..17320
                     /locus_tag="PW224_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2908040.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="PW224_001342"
     CDS             17405..18289
                     /locus_tag="PW224_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2908041.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="PW224_001343"
     CDS             18403..18639
                     /locus_tag="PW224_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL2908042.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="PW224_001344"
     tRNA            18727..18807
                     /locus_tag="PW224_001344"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="PW224_001345"
     tRNA            18814..18885
                     /locus_tag="PW224_001345"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="PW224_001346"
     CDS             19011..19181
                     /locus_tag="PW224_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908043.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="PW224_001347"
     CDS             19401..20108
                     /locus_tag="PW224_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2908044.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="PW224_001348"
     CDS             complement(20167..20811)
                     /locus_tag="PW224_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL2908045.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="PW224_001349"
     CDS             20938..21225
                     /locus_tag="PW224_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908046.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="PW224_001350"
     CDS             21333..21653
                     /locus_tag="PW224_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2908047.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21971..23047
                     /locus_tag="PW224_001351"
     CDS             21971..23047
                     /locus_tag="PW224_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2908048.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23044..23763
                     /locus_tag="PW224_001352"
     CDS             23044..23763
                     /locus_tag="PW224_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL2908049.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23891..24442
                     /gene="raiA"
                     /locus_tag="PW224_001353"
     CDS             23891..24442
                     /gene="raiA"
                     /locus_tag="PW224_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL2908050.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            24664..27198
                     /gene="secA"
                     /locus_tag="PW224_001354"
     CDS             24664..27198
                     /gene="secA"
                     /locus_tag="PW224_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL2908051.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            27324..28425
                     /gene="prfB"
                     /locus_tag="PW224_001355"
     CDS             join(27324..27395,27397..28425)
                     /gene="prfB"
                     /locus_tag="PW224_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL2908052.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            28530..29216
                     /gene="ftsE"
                     /locus_tag="PW224_001356"
     CDS             28530..29216
                     /gene="ftsE"
                     /locus_tag="PW224_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL2908053.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            29200..30093
                     /locus_tag="PW224_001357"
     CDS             29200..30093
                     /locus_tag="PW224_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908054.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            30245..31693
                     /gene="cls"
                     /locus_tag="PW224_001358"
     CDS             30245..31693
                     /gene="cls"
                     /locus_tag="PW224_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2908055.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        10607 a         5565 c         6908 g         8816 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa agattacccg gcatatattt attgtccaat acctttatct
    23461 gaaaaaaggt ggctagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaaa
    23521 attccttttc agatgctgct caaaagagag caacatctgc ttgctcaagc aaaaaacacg
    23581 cgaaaggaaa gattggagat gcctcagcct tttgagcttg ccgtacctct ttctaagata
    23641 caacataaaa aaatattgat tattgacgat gtctatacaa caggacagac gctttttcat
    23701 gcagcagatt gcttgcagaa cgcacagccg aaaatcatcc gaaccatttc tttggcaaga
    23761 tgaatcagat gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata
    23821 atagaattaa gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg
    23881 ggtaattttt atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat
    23941 tcgcgactac gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga
    24001 tgcaacggcg catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac
    24061 gatcccatta ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag
    24121 tatcgatttg gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa
    24181 tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct gctgctttat tcaatgaaga
    24241 ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa
    24301 accaatggat agtgaagaag cagtcttaca aatgaacatg ctgggacata acttctttat
    24361 ttttgaagat gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata
    24421 cggactgatt gaaacagact gatcagcctt acttaaataa caacgaaaac ttgcagcagc
    24481 tactgacagc tgccgcaagt ttttttgagt ttcctcctct tttagatgaa atatgaataa
    24541 attatgaagt tcttacttta atacgcttaa gatatttcaa ttatctataa ctaatgataa
    24601 aatgaaaagt aaagtctaca tagtctggat aatacacaga aggaaaagga gattacacaa
    24661 cgcatggcca atttgattcg ttcacttatc gaaaacgata agaaagaact aaaacgttta
    24721 gatagcattg caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt aactgatgaa
    24781 caattacagg caaaaactgc tgaattcaag ggcagatacc aaaaaggaga aacattagat
    24841 caattacttc cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt attaggttta
    24901 tatccatacc acgtacaatt gatgggtgga atcgtgcttc acgatggaaa tatccctgaa
    24961 atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct aaatgctctg
    25021 actggcgaag gggtacacgt agttaccgtc aatgaatatt tagcaacccg tgactcgacg
    25081 gaaatgggcg aactttataa tttcttagga ttaacagtgg ggttgaatat caactcaaaa
    25141 tcttcggatg aaaaacgcga ggcttataat tgtgatatca cttatagtac gaataatgaa
    25201 ttaggatttg actacttgag agacaatatg gtcgtttacc gccatcaaat ggtacaacgt
    25261 ccgctgaatt atgccattgt cgatgaagtt gactcaatct tgattgatga agcgagaacg
    25321 ccgctgatca tttcagggca agcagaaaaa tccacagctt tatatacaag agcagacaac
    25381 ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg atgtacaatc aaaaacaatc
    25441 agtttaaccg aagcagggat cgaaaaagca gaagaaacat tcggtttgga taacttatac
    25501 gacattgaaa atactgcatt gacccatcac ttggatcaag cattgagagc caactatatc
    25561 atgcttttag atatcgatta tgttgtccaa gacggaaaag tactgattgt tgaccagttc
    25621 actggtcgga tcatggatgg acgccgttat tcagatggac ttcaccaagc aatcgaagct
    25681 aaagaaggcg tagagatcga agacgaaacg aaaaccatgg cgacgatcac tttccagaac
    25741 tatttccgta tgtacaagaa acttgcggga atgactggta cagctaaaac agaagaagaa
    25801 gaattcagag aaatttataa catccaagta taccagattc cgacaaatcg tccggttatc
    25861 cgtgatgatc gtccagattt gctttaccca acattacaaa gcaagttcca cgctgtcgtg
    25921 caagatatca aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt
    25981 gaaacatctg agttattatc agatatgctg aatcaagaac gtattcctca tgaagtgctg
    26041 aatgcgaaaa atcactttaa agaagcagag atcatcatga atgctggtca aaaaggtgct
    26101 gtgacgattg ctaccaacat ggctggtcgg ggaacagata tcaagttagg acttggggta
    26161 agagaacttg gtggtctagc agttatcggg acagaacgac atgaatctcg tcgtatcgat
    26221 aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca gttctacttg
    26281 tcattggaag atgatttgat gaaacgattc ggttcagaac gaatcaaagc tttcttagat
    26341 cggatgaaga tcggtgagga agatgctgtt atccaaagca agatgctgac gaaacaagta
    26401 gaatctgcac aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa
    26461 tacgatgatg tgatgcgtga acaacgtgaa gtaatctata aacagcgaca agaagtaatc
    26521 atggaagaaa aaagcttatc caagccacta atgaacatgg tcaaacggac aatcagtcgt
    26581 gtagtagatg cgcatacaca attagagaaa gaaaattgga atttggaaag tatcatcgat
    26641 tttgccggca gtactcttgt ccatgaagat agcattactc ttgcagatat cgaaggaaaa
    26701 acagctcagg agatcaaaga gtatctaaac gaacgagcac aagaagtatt taataccaaa
    26761 gcggctcaac taaatggtcc agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt
    26821 gttgtggact ctaaatggac agaccatatt gatgcaatgg atcaattacg tcagtcaatc
    26881 ggcttacgtg cttatggaca gaacaatcca ttagtcgaat atcaaacaga aggctataag
    26941 atgtttgaag atatggtagg cgccatcgag tatgaagtca ctcgaatctt tatgaaagct
    27001 gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc tcactcagaa
    27061 gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat ccatgtgaat
    27121 aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa gaactgtcat
    27181 ggaaaaaatg caaactaatt tctgacagca ggattggtga gacaaattct ttttttaaga
    27241 attcgtctca ccttctttct gttatttgct gattctgtta gactagatat aatgaaaaaa
    27301 gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa
    27361 tgcaggaaaa ggtcacaagt ttcaggaggt ctctttgact tagatcagct ggaagaagac
    27421 atcgcggaag cagaagcaaa aatggctgaa cctggttttt gggatgacag cgaagccgca
    27481 caaaaagtga tcagcgaaaa taacgctaat aaagaaacct atgaccaatt ccattctctt
    27541 gccgaagaac tagaagaaat cgaattgatg gtggagatgc tgcaagaaga acctgatgca
    27601 gcaatgcaag aagaagcaga aacacgtatc aatgcattag atgaaaaaat gaagacgtat
    27661 gaattatcta tgctgttaga cggaccttat gatcgaaaca atgcgatcgt cgaattgcat
    27721 ccaggggctg gcggaacgga gtcccaagac tggggcagca tgcttttacg tatgtataca
    27781 cgttgggcag agcagcatgg ctatcaagta gaaacactag attatcaagc aggtgatgaa
    27841 gcagggatca agagtgtgac cctgctgatt aaaggacata acgcttttgg ttatctaaaa
    27901 tcagaaaaag gtgttcatcg ccttgtacgg atttcgcctt ttgactcagc gaaacgacgt
    27961 catacttctt tttgttcggt agatgtgatg ccagagcttg atgaaacgat cgatatagaa
    28021 atcaatccag atgatttgaa aatcgatacg taccgagcaa gtggtgcagg tggtcagcac
    28081 atcaataaaa cagaatctgc tgtacggatc actcacttgc ctacaggaac agtagtagct
    28141 agtcaggcac aacgctcaca attgaaaaac cgtgagcaag cgatggggat gttaaaagct
    28201 aagctttatc aattagaagt ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa
    28261 caaaaagaaa tcggctgggg ttcacagatc cgttcttatg ttttccatcc ctattctatg
    28321 gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc aagcagttat ggacggtgac
    28381 cttgatttat ttattgacgg ctatttaaaa atgcatctac aataagaacc cattgttaaa
    28441 ttgaaataaa attgtaagat tttgcaagca tgttgaaaca ttgccatgct ataatagcta
    28501 aagactgaaa aagatatgga gagaacgcca tgattgaaat gaaggatgta atgaagaagt
    28561 actcaaatgg tacgaccgct atccgcaatc tctcgattgt catcgaacaa ggagagtttg
    28621 tctatgtggt tggtccgtct ggagccggta aatctacatt catcaaattg atgtatcgag
    28681 aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca tgatctaata gcaatcaaaa
    28741 accgcgaagt tccttatcta cgcagagaaa tcggcgtggt gttccaagac tataaattat
    28801 taccaagaaa aacagtatat gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac
    28861 ctcgagatat caaaaaacga gtaatggaag tgctggacct tgttggtttg aaacacaaag
    28921 ttcgagtctt tcctagcgaa ttatccggtg gggaacagca acgtgtctct attgctcggg
    28981 caatcgtgaa tacgcctaaa gtacttatcg cggatgaacc gacaggaaat ctggatccag
    29041 aaaactcatg ggaaatcatg aaattattag atcggatcaa tgcccaagga actactgtcg
    29101 tgatggctac acataacagt acgatcgtaa atacgattcg gcaccgagta attgctatcg
    29161 aaaacggccg cattattcga gatcaagcgg aaggggaata tggatacgat gattagaacc
    29221 tttttccgcc atttattaga aagtatcaag agtttgaaac gtaacggctg gatgacagtt
    29281 gcttcagtca gtgcggtaac gatcacgttg actttagtcg gtgtattcat ggcagttatc
    29341 atgaatgcaa ctaaattagc tcaggatata gaaggaaacg ttgatgtatc tgtattcgta
    29401 gatatcggta caaaagacga agagatgaaa aagctagaaa aagaattgaa tgacttagat
    29461 catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat tgaaaaaaat ccaaaacgaa
    29521 atgggtgatt cctggaaatt gtttgaaggt gacaataacc cgttatatga tgtgtatgta
    29581 gtaagtgcta ctgatccttc ttatacaaaa caagtagcag aagaagcagc agatttgaag
    29641 aatgtttctc gttctgatta cggtggagct tcctcagatc ggattttcca aatcgctggt
    29701 gcagttcgga catggggatt agttgcagcg gcattgctgc tgttcgtagc gatgtttctc
    29761 atttcgaaca cgattcggat cacgattttg tctagacagc gtgaaattca aatcatgcgt
    29821 ttagtcggtg caaaaaatgg atatattcgc tggccgtttt tccttgaagg tggctggatc
    29881 ggtctgatcg gtgcaattct tccaattttg atcatcacat ttggttatgc atggtttttc
    29941 caattgatca atccatcatt attacgttcc cattattcat tgattcatcc acaaaatatc
    30001 gtttggaaaa tcaatctttt aatggtcggc attggtgtga tcattggttc acttggctcg
    30061 atcatttcta tgagacgttt cttgaaattc taataccaca atataaataa aatgagaaag
    30121 ttggacagtt tcgttccagc tttctttttt tattggctag gagaatagaa ggaagcggct
    30181 tttttccctt tactttaaaa aacaacttgg gtattcttaa gttggtagaa gaagaggtga
    30241 agccgtgaaa attatcgttg acaatttttt tacgatcctt ttgattatga atattttgct
    30301 ttcctttatc atcgtttttc gagaaagaaa ggaaactgct caaacatggg catggctgct
    30361 cgttttgatg ttcattccag ttgtaggatt tattttatat atttttttag gaagagggat
    30421 ttccaaagag aaaatctttg atttgaagat ccaagggaaa atcggtaaga acctggaaat
    30481 tgaagaagac aaacaggcat tgatgcgagg gctttatcct catccgccta ctggacaagt
    30541 agatgtgaaa caattgatct atatgctcac ggtatttgaa agtacgctct atacgactaa
    30601 aaatgaaatc actttattta cagatggtcg tgaaaaattc gatgcgctga ttgaagatat
    30661 caagcaggca gaagaccata tccacttcca gtattatatt tatcgcagtg atgcattagg
    30721 tgaagaagtt cgagatgcat tgacagaggc ggcaagacga ggggtaaaag tcagagtcct
    30781 tcttgatgca tggggttcca cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc
    30841 aggcggagaa gtcgcatttt tctttcctct atttgttccg tatattaatc ctcggatcaa
    30901 ttatcgcaat catcgtaaaa tcgtagttat tgatggggaa atcggatata caggtgggtt
    30961 caatgttgga aatgaatatc taggattggt aaagaaattt ggttattggc gagacaatca
    31021 tttgcgtatt tacggagaag cggtgtatac cttgcaaaac cgttttttga tggactggaa
    31081 ttctcagcat acaaaagaag taggctattc tcccaaattt tttccgtcca ttcacactac
    31141 tggtgatata gctgttcaaa tcgtgacaag cggaccagat accgaacatg aacaaatcaa
    31201 aatgacttat ttgaaaatga tttcgttagc caaacgggag atattgatcc aaacgcctta
    31261 ttacataccg gatggatcga ttcatgaagc actgaagttg gctcttcttt caggcgtaaa
    31321 agttcatatc caaataccta acaaaccaga tcatcttctc gtttactggg caacgtattc
    31381 tttcgctgca gagctgatcg agtatggagc aaggatcgaa acatatgaga atggatttat
    31441 tcatgcaaag acgatgatta tcgatggtgg cattgtttca gttggctcag caaatatcga
    31501 tgttcgttca tttcgcttgg actttgaggt caatacactg gtttatgatg aacgaatggc
    31561 ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga
    31621 aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa gattgatttc
    31681 gccgctcctt taaaacataa aaaggacatg agaaccatca acagatggag atctcgtgtc
    31741 cttttttaac ggggccattt ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag
    31801 cgaaattaac tttggggtat ctcttgtttt ttggggaaag caagaaataa gctaatatat
    31861 gctattaaaa gagacacatt tcaaagccag tatagc
//
LOCUS       DANKMN010000032        31780 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000032 DANKMN010000000
VERSION     DANKMN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            661..1530
                     /locus_tag="PW224_001359"
     CDS             661..1530
                     /locus_tag="PW224_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL2908056.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1739..2764
                     /locus_tag="PW224_001360"
     CDS             1739..2764
                     /locus_tag="PW224_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2908057.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2803..3753
                     /gene="manA"
                     /locus_tag="PW224_001361"
     CDS             2803..3753
                     /gene="manA"
                     /locus_tag="PW224_001361"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL2908058.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3847..5550
                     /locus_tag="PW224_001362"
     CDS             3847..5550
                     /locus_tag="PW224_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2908059.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5552..7585
                     /locus_tag="PW224_001363"
     CDS             5552..7585
                     /locus_tag="PW224_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2908060.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7596..8036
                     /locus_tag="PW224_001364"
     CDS             7596..8036
                     /locus_tag="PW224_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2908061.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(8236..9363)
                     /locus_tag="PW224_001365"
     CDS             complement(8236..9363)
                     /locus_tag="PW224_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908062.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9366..10043)
                     /locus_tag="PW224_001366"
     CDS             complement(9366..10043)
                     /locus_tag="PW224_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2908063.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            10307..12073
                     /locus_tag="PW224_001367"
     CDS             10307..12073
                     /locus_tag="PW224_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL2908064.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            12088..12525
                     /locus_tag="PW224_001368"
     CDS             12088..12525
                     /locus_tag="PW224_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2908065.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12538..13689
                     /locus_tag="PW224_001369"
     CDS             12538..13689
                     /locus_tag="PW224_001369"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL2908066.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13751..14449)
                     /locus_tag="PW224_001370"
     CDS             complement(13751..14449)
                     /locus_tag="PW224_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL2908067.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14442..15794)
                     /locus_tag="PW224_001371"
     CDS             complement(14442..15794)
                     /locus_tag="PW224_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL2908068.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(16017..17417)
                     /locus_tag="PW224_001372"
     CDS             complement(16017..17417)
                     /locus_tag="PW224_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL2908069.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17578..18315
                     /locus_tag="PW224_001373"
     CDS             17578..18315
                     /locus_tag="PW224_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL2908070.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18440..18919
                     /locus_tag="PW224_001374"
     CDS             18440..18919
                     /locus_tag="PW224_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL2908071.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18916..19683
                     /locus_tag="PW224_001375"
     CDS             18916..19683
                     /locus_tag="PW224_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908072.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19913..20482
                     /gene="lepB"
                     /locus_tag="PW224_001376"
     CDS             19913..20482
                     /gene="lepB"
                     /locus_tag="PW224_001376"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2908073.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20586..21188)
                     /locus_tag="PW224_001377"
     CDS             complement(20586..21188)
                     /locus_tag="PW224_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2908074.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            21294..21763
                     /locus_tag="PW224_001378"
                     /pseudo
     CDS             21294..21763
                     /locus_tag="PW224_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21869..22453
                     /locus_tag="PW224_001379"
     CDS             21869..22453
                     /locus_tag="PW224_001379"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2908075.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22456..23529
                     /gene="prfA"
                     /locus_tag="PW224_001380"
     CDS             22456..23529
                     /gene="prfA"
                     /locus_tag="PW224_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL2908076.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23522..24361
                     /gene="prmC"
                     /locus_tag="PW224_001381"
     CDS             23522..24361
                     /gene="prmC"
                     /locus_tag="PW224_001381"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL2908077.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            24354..24533
                     /locus_tag="PW224_001382"
     CDS             24354..24533
                     /locus_tag="PW224_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908078.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24555..25580
                     /locus_tag="PW224_001383"
     CDS             24555..25580
                     /locus_tag="PW224_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL2908079.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25771..27015
                     /locus_tag="PW224_001384"
     CDS             25771..27015
                     /locus_tag="PW224_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL2908080.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            27168..28115
                     /locus_tag="PW224_001385"
     CDS             27168..28115
                     /locus_tag="PW224_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2908081.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            28249..28878
                     /gene="upp"
                     /locus_tag="PW224_001386"
     CDS             28249..28878
                     /gene="upp"
                     /locus_tag="PW224_001386"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL2908082.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(29017..29259)
                     /locus_tag="PW224_001387"
     CDS             complement(29017..29259)
                     /locus_tag="PW224_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908083.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            29380..29452
                     /locus_tag="PW224_001388"
     tRNA            29380..29452
                     /locus_tag="PW224_001388"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29413..29415,aa:Thr,seq:cgt)
     gene            complement(29571..30209)
                     /locus_tag="PW224_001389"
     CDS             complement(29571..30209)
                     /locus_tag="PW224_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2908084.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(30332..30964)
                     /locus_tag="PW224_001390"
     CDS             complement(30332..30964)
                     /locus_tag="PW224_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2908085.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            31315..31614
                     /locus_tag="PW224_001391"
     CDS             31315..31614
                     /locus_tag="PW224_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2908086.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10823 a         5573 c         6379 g         9005 t
ORIGIN      
        1 tcgtgtcaca gtcccttttt ccgaataaac ggtgttcaat cagctgtcct tcggtattag
       61 ctcaaaaaac ggcaaaatat gagaagctat ttttccgttt tgtatctcta atactcaggt
      121 caaaccgctg attttacaac ctcttcctta aacggtgttc tcctgccaac tcctcggtca
      181 caagtcacaa tagtcaacaa acctgagaag ctgttttttg aaattgctcc ttgtgactcg
      241 gagttaaacg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca gtttctcggt
      301 attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt tcttactact
      361 cgaaactaag cgactttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg
      421 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
      481 tacttgatgc agaacagctt tttcacaacc tctttttata agagttttta ctcattaatt
      541 tacaaaaaac aagcaaaatg cgaattttct cacatttttt ttaacaaaat caaagttatt
      601 gcttgaaaga agaagaatcc atcgttaaaa taaagcttga cgtagagggg agcaagaaat
      661 atgaaagatt ttcaattggg ggctgccacc tatttaaaaa gtgtagccat tcgggtgaaa
      721 gaccgagata aaatgattga tttttacaag aacgttgttg gctttgattt gaaaagagaa
      781 gaaaacgaat tagccatttt aggaacagaa gaagctggaa gtgaaatgtt gctgctggaa
      841 gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa agctgaaccg tttttcattg
      901 attatcccta gcacagaaga aatggcagat atcctttgtc gtatccgtaa aatgaattat
      961 ccaatcaaag aagctttaga agatcatgga cgtatgggga ttttgctcgt agacccagaa
     1021 gagaatgaat tggaaattta ccatgaagag aataagaacg accaaagaga aattccagaa
     1081 acaatggatc aagaagcatt attaagaaaa tctgaagaaa aattcagtaa actatcaaca
     1141 ggttcttatt ttgaaaaaat ccatttaaat gttactaact taacaaaaga acgccaattt
     1201 cttaatcatg tacttggttt gaaggtacat aatgaagaag agggcatcca tgtattgaat
     1261 gaaggagatt tccatgttgg tttgacagaa gctagcggag gcgcaattga tttgcctaca
     1321 gatgaagtga ttggacttga ttttctgaaa ttcatgatcg atactgaaag catggaagga
     1381 ctgattgctc acttgcagca actatcagtt gatttcttct tagacaaaaa gcacacagta
     1441 ctcacaatct atgatgctat aggaatcgaa tggtggttcg tcagtgagcc aaaagaaaag
     1501 aataaaactg acattcgatg cgacgcttaa ctttttcttt cttaacgatt gtcgcatgtc
     1561 tcgtcatttt atgaacggtc atactatcgc ttattctaga gatagaaaga gaaaatagta
     1621 taccggggga aactgcgcgg tttttcgcgc agtttttttg tgtaaaaaag gttgattcct
     1681 catagattaa gctttaagct tggactgtaa gaatctgata gaaaagcagg agaaaaagat
     1741 gtatccacac acgaaacagc ttattttgaa aaagatggaa gaaggatttt atccaggtgt
     1801 agcagtcgcc ttcataaaag aaaatcaagt agaaacattt acagccggct ttgcccaagt
     1861 cgttcctgaa aagcagctga tgacagaaga tctgttgttt gatgcagcct cactgacgaa
     1921 agtcgttgca accacttcat tagttttacg tctattagaa gatggaaaga tagaactcga
     1981 tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag acaatcactt tgcgtcattt
     2041 actgacacat acttctgata tcaaaacatg gataccaaac agagataagc tatctcagga
     2101 agaattgatc gccgcttatt tgaagctaga accaggtgag caactaggaa aagaagtaaa
     2161 atatacagat gccggtacga ttcttcttgg gttgatgcta gaggaaattt accaagaaac
     2221 tgcagtggaa atctttcaag aacaagtatt ggaaccatta ggaatgatga acagcttatt
     2281 tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa ctgccttcgg gtgaggtttt
     2341 gcgtggacaa actcatgatc cgaaagcaag agttttggct gaacatgcag gtaacgcggg
     2401 tctctttatt gatttgaaag acagcattac ttttgtccaa atgtatttga acaagggaaa
     2461 aatccgtaat ggtcgttttt tagaagaaga gacgattgat tctttactgc aagatcaaac
     2521 accgaaaaaa aatggctatc gttctttagg atgggactta aaaaaggatg tagaagacgg
     2581 tcatgcgatt ctttttcata ctggttatac aggtacgttt ttcttgattg atataaaaac
     2641 acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct gtcgatcatc gtgcggagta
     2701 tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa gagaaagcgg tattaccgaa
     2761 ataatgctat aatagctatg aaaaatgaga ggagtttgaa ttatgcagga acctatgttt
     2821 ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc gtttacgttc agtctttggt
     2881 tttgatatcc ctaacgataa aatcggagaa gattgggcga tcagtgcgca tcctcatggt
     2941 gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat tagatgaact atggtcagaa
     3001 cacaaggaat tgtttggcca tccttcagaa ccagtgtttc cattattgat caagatctta
     3061 gacgcagaag atgatctatc tgtacaagta catccagatg atgcctatgg gatgaaacat
     3121 gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg atgctgaacc tggtgcagaa
     3181 atcatctatg gtcatcatgc aaagacaaga gaagaactag ctgagatgat caaagatggg
     3241 cgctgggatg accttttgaa gaaagtacca gttaaaaaag gtgatttctt ttatgtacca
     3301 agcggaacga tccatgctat tggtaaagga atcatgattt tagaaacaca gcaaagttct
     3361 gacacgactt accgcgtgta tgactacgac agaaaagatg cgcaaggaca aacaagagag
     3421 ctgcatatcc aacaatcgat cgatgtaaca actgtccctg cgaaaacacc agaattacaa
     3481 atcaaagaag tccgaaaagg gaattcatcg atcgtcactt atttagaaac tgagtttttc
     3541 aatgtatatg agtgggatat taaaggaatc acatctttca aaaaacaagc cccttataca
     3601 ttgatgacgg taatcgaagg agcaggagac cttgtcgttg acggaaaaac atatccatta
     3661 gaaaaaggaa caagctgtat tattccaaat ggcgtttctg agtgggcggt ccaaggtgaa
     3721 ctagcaatca ttgcgtctga accaggcaaa tagttcccag atagctgaaa gcagcagacg
     3781 gtttttcgtt tgctgctttt tctacataaa atccgtccat caataaatag aagtctggtg
     3841 aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc gcgtgtctat gaaacaactg
     3901 agaaaagctg gacatcactt aaatgaaatg atcatgccaa atctaagtct gtttatcgct
     3961 tggggaatct tgtcattagc aacaaaaggt gtgtctggcg atttgcacac cacattagaa
     4021 gaggtatgcc attggatgat ccagctgctt ttacccatct tgatcagtta ttcaggcggg
     4081 aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa ttgcttcttt agggttgatc
     4141 gctcgttcca atgctccaca aatcgtagga gcgatgatta tcgggccgct tgccggcatc
     4201 gtatatgaac aatttgtttc tagattccat gtgaaattca aggctggata tgaaatgctt
     4261 gtcagcaacc tactagtagg actgactggt agcgttattt gtataattgg aatcgttttt
     4321 attgaaccag ttttaaatag tgttcacttg ttgctcgcct ccgttgtgtc ttggctagtc
     4381 acaatgaatc tgttaccact tgtcagtctg atcttagaac ctctaaaagt actatttttc
     4441 aataatgcaa tcaatcatgg tgtattgaca cctttaggaa tcgaattctc acaaacagtc
     4501 gggtactctt ccttattttt gatggaggca aatcctggac ctggtttagg gatcttactt
     4561 tctatccttt gttttgcaga aaaacaggaa aaagtaaatg cgagtggagc attgatgatc
     4621 catgcgattg gcgggattca cgaaatctat tttccatttg tcttgctgcg accgtatcta
     4681 tttttagctg taatggctgg tggggcaagc ggcacgttga tatttcaatg gttagatacg
     4741 ggattagctg cacctgtatc gcctgggtca ctactcgtga tttttgccaa tgcgccttat
     4801 ccacaactag ctggggtaag tatagggata gcaatcagta catttgtgac cttcatttgt
     4861 gcagcgatac tattgaaatt accaaataaa gaaaaagaga acaaaacggt caaagaaagg
     4921 ggagaaatct tggaaccatc tatcattcat tccgtcattt ttgcttgcga ctcaggtatg
     4981 ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat tagcagcaga aaaaatcgat
     5041 ctttctgtcg gatacagctc tatctatgaa ttgaataacc aagtaaatca gctggttatc
     5101 gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac ctcaaagtca aatctatgta
     5161 ctggatcact ttttggaaac agatatcttg ctgtcacgaa taaaagaagc gatccaattt
     5221 ccaaagcggg aagaaaaaaa agagatagca gatatcacag aacaaaaaac gtcgcctgct
     5281 aatattgtat ttttgtataa aaacaacaga cgcggctcac aaactatggg catgacgatt
     5341 ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta tctcaaaaga atcaatagaa
     5401 aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa gagaactagc tgtaactgaa
     5461 cagctagaag aaaagatccc tcggctagtt ctagttgaac attgggtagc tacgaatgaa
     5521 tacgaaaaac tcgtgaaggg agaaatataa gatgtattta tcgcccagag aaaaacaatt
     5581 attagcagaa tttctcagta gcccgtcacc tgtatctatc caaaaaatga tgaatttatt
     5641 gaaggtcagt aaacggacta tttaccgcga attagataat cttgaagtct cattgaaatc
     5701 ggtaaatgct tctttgaaaa aagcagctag aggctcattt gtcattgagg catcagaaga
     5761 agtattagag caattaaaag aaaatacaga tgggaaaagt aatgacttat ccactgctaa
     5821 acgacaacac gcgattttag ccgagttgtt actgacgaca gagccgttga gtcttgctca
     5881 tttcatggaa tcatatcgca tcagtaatac cactttttat gcagatatca aacagttgga
     5941 agaaagtatt cgccaactgc ctttggaaat cattcggaac cagggatacg agatcgaagg
     6001 accggaaaaa taccgccggc ttctgacagc gaatgtatta gaattagaaa tcaatgaata
     6061 tgaacttttt catagtattt ctttcgatgc atcactgaat tatttttttc gattcgttga
     6121 tccgcaacat ctatctcttg cacgcaaagt aatcggggaa gaattgatcc aaaaaaagac
     6181 caatctaagt gatagaaagc ttgaacattt ggttttgatg ctgacattga cgatggagcg
     6241 tgtgacgaaa aatcatttac tgacaaatga gacatataat agtctggcaa acaaagaatt
     6301 actacagaca gctaaacgat tgttttcacg gatcgcttct gagacaaaac aactttatcc
     6361 agtaaatgag atcgtctttt ttgcaggatt attaaatgac ttcacgaact catttgatca
     6421 gaatttcttt gaagagacgt tcgatacaca gcttgcttat ttagtaaaac aactgattca
     6481 agaagtaagt gaagagacga atgtccattt ttatgaagat gaaactttgt acaaaatgct
     6541 gttgactcat ttatctggtg tgttttcaag agccgtttta caagaagaga cactgaccaa
     6601 tccgatttta gaaaaaatca tggttcaata cgaggaagtt gcgaatgcaa tccgtaaagc
     6661 agtagagcgg atttttcaag aaaaaaaatt atccgaagaa gagatcgctt atatggtcct
     6721 tcattttgcg aattcattgg aacgaagtcc gaaagaaagc aatatcagta ttgcaggatt
     6781 ttcgccaagt ggattagctt ctactagcat gttggaacta cgattgaaaa ggtattttcc
     6841 cttcatccaa caaattcatt tctatcggat tgctgattta aaaaaaatcg atttacaaga
     6901 agaatatgat gttgttattt ctacatctat tttaccagga tacggtggaa aatatatttt
     6961 agtctccccg ctactgctag aagatgaagt caagcagcta aaagaagctt tttcgcatat
     7021 tgagaagaaa aaaaggcaaa aaattacgta taacagcaat aacgagtgga tgatagaaga
     7081 tacttatgag gaaacaatgc atttcatgga tcaaatcaat caattactcg gtcgtttttt
     7141 tatccaagaa ctggataatc ctgagacgat ttccgaaacg attcgcagct tatttcctta
     7201 ttttcctgaa ggactagtaa cggatgaaga ggtggttcat cgccggctgc ttaagcgata
     7261 tgaacaagca cctatcggta ttcctcatac aaatatgggg ttattccata catccagctc
     7321 attgatttct aaaccgatct tttgtatttt caatttgaaa catccgttga ccattgaagg
     7381 gatggataga cagccagtcg atttgtcccg tatgttagtt atgctggcgc cttcgccaat
     7441 ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga gcaatcatca tgaatgactt
     7501 gaacaccgag atcttccgct caggaaataa agaaatcgtt tatcagttgt tagcgaaaat
     7561 cctgattgaa gaaataaaga aatgaaggtg aaaagatgat acagctacca gcagaaaaaa
     7621 tattgctcga tcaatcctat caaaattgga aagaagcgtt gaccgagatc ggccagtgga
     7681 tgacccaaca agggggaatc aaaccggcat atatccaatc aatgatcgaa agacaagaaa
     7741 aagcgagtgt ctatatcggg aatttcgtag ccttgccaca tgggactgac aaagatgaag
     7801 aagtagtaga agaaggaatc tatttagtcc aagtgcctga tggtgtggac tttggaacaa
     7861 aagaggaacc aaaaatcgcg acactcttat ttgtagtggc tttaaaaaaa gaacatcagt
     7921 tagcggtgct ccaagattta gcctttttct gttcagacat cgaccaagta atggcgctca
     7981 gtgatgcgga aaccattgaa gaagtccaaa tgatttttga acatggacat atataaccaa
     8041 tgttcacagt aaaaaaagct gccttgcatc aagtaataac ttcaaccaag ataaaaataa
     8101 gaggagctat ttttgttggg aggttcttat tactgcggat gcaagctttt gaaaactatt
     8161 tattctagaa aaccgtttat ttgaaatagg ggacggtgac aaggtttttg tcaccgcccc
     8221 tattattttt gtgttttaat agctcttttt tatatagata gtgcccatac ctatgaataa
     8281 taaaacgata ctccatatac caagtgtcaa gataccattc atgaaagtaa cctgttctga
     8341 agctagtggc gagaaaatat ctcctcctat gatcacattt tgaaaatcaa cataagaagt
     8401 tggcaagtaa tgactgattg attctatcca tccaatcgaa gaaaacaagc gatactctgc
     8461 taagaaaaga caaagaagtg tacctaaaag attgactaga gaatgttcaa ataaagcaga
     8521 tagaagaaaa ctgattgtac ttaaaaagag cacccacaac aacaggaaca gaaagctctg
     8581 gaaaagatac tcatacatcg gcaggattcg tatctcctga ccaatgatcg ttcctacagg
     8641 ataacggaag tctcccaaac catttttgat tattactagt atagagacga tcaaaaatgg
     8701 catcacgaga cttagcatag ctgcaccata ataaatggta aaacggatgc tatgtttctt
     8761 taccatacta tccagtaata gattgactag agagattacc tgctttcttt tttcataaga
     8821 gatgatggaa gaaagaaaca aggcggttat acataaaatc aaaaaactag aaatcattcc
     8881 tgaaaaaata ttttcgtagt aatttgctaa agagagatgc gctggcaagt caaaaataga
     8941 atatctttgg atctgatgtt ctttcatata acggagcaac tcgacacgtt tttgttgttc
     9001 aatgattgga attccttgtc tttgaccaga aatcagtcga tccagtatat ctttttcata
     9061 atgataagtt gcttcgacca tttgctggac attccctgtg ttttttgcat gtaagattgc
     9121 ttcgatcagt ttgttgcttt tctctaaact ttccacaaat tctttctcat tctcatcttc
     9181 tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta tgattcagtt catcgaattc
     9241 tgtataagca agtttatgat caaaaaaaag tgggaatgca aagagcacga ccaacaatag
     9301 aggaaataaa aagtttactt tgttttttag taagagattg cgttcaaatg taagtaaaga
     9361 catagttaca cctcatataa tttacgataa atagctaaaa tctcttccat atttgaggat
     9421 tctgtgacga tttctccatt ttttagaaaa catgctttag aacaaatcga ttggatattc
     9481 gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt tctgccattg tctgatgagg
     9541 ctattcaaga tatcaatact tcctgggtct aatccgttca acggttcatc gaataacatg
     9601 acagatgaat cactgatcaa gtaagcagca agcaagacat gttgtttcat tcctagagac
     9661 atttttccaa tcggcaaagt aaggtactca gtcatcttca atcgttggaa aacagtttgg
     9721 cattctactt tgctctgcca atagtatttg atcaaagtca aataatcaac agccgataga
     9781 tagtcggata actgattcgt tgattctaga aaaaagaaag accggcgata atctgtataa
     9841 gcagtaggag agatgttgtg aagggacaat tgatcataag ttgttggcag cagttgggca
     9901 atgccattca gcaaagttgt tttgccaaat ccgttcgggg caatcaaagc agctacttct
     9961 cctgattcta ctaaaagatt caattgtcga atcacacgtt ttttaccacg tttcatgttc
    10021 atgtttttga tcagtaatgt catagatagg cctccaatat aaaatttaat aacataatag
    10081 aatggaaaac atctaaatta aatgctaaca aaagttattg tttgcatatt ataaaaaaat
    10141 taaaataaca gttttattta aaattgaagt tttctgatac cgaaatcttt ttattttgct
    10201 gacatactca aacctttggc acaaagtttc gtgccaaagt acgactattt ctctttttag
    10261 taagcggata caattagagt atcaaaagga gagagggaga gacaaaatgg aacggacgat
    10321 tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa ttaggcagta ctttatcaag
    10381 catggtcatg ccaaatattg gagcactgat tgcctggggt gtattaacgg ctctatttat
    10441 cccagatggt tatttaccaa atgaggcttt cgctaccatg gtaagcccaa tgctaactta
    10501 tctgattcct ttattgatcg gttatactgg cggaaaagta attgctggtg accgcggatc
    10561 agttgttggg gcaattgcaa cgatgggtgt gattgtcgga acagatattc caatgatgct
    10621 aggcgcgatg atcatgggac ctcttggtgg atatgccatc aagaaatttg atcagctatt
    10681 ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat aatttttcag caggtcttat
    10741 cggttttgga ttagcgttat taggtttttc tgcgattggt ccagttgtcg atgcattgac
    10801 tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc catttgattc cattgacaag
    10861 tatttttatt gaaccagcaa aaatcttatt tttgaataat gcaatcaatc atgggatttt
    10921 gacaccattg ggaactgaac aagtagcaga ggctggaaaa tctattctat tcttgcttga
    10981 agcaaatcct ggtccaggac ttggcgtatt acttgctttt actttgtttg gcaaaggagc
    11041 agcaaaatct tcagcaccag gagccatgat cattcacttt ttaggcggga tccatgaaat
    11101 ctattttcct tacgttatga tgaaaccgtt attattctta tcagttatag gcggaggaat
    11161 gtcaggaagt ttcattttcc aattgatgga tgctggacta agagcgccag cttctccagg
    11221 gtcgatcatt gcgattctag cgatgacacc gttcaattct taccttcctg ttattttggg
    11281 cgtgattgtt gccgcagctg tttcgtttgg tatttctgca gtaattttga aagcggatgc
    11341 gaaagataca aatgaagact tcgaaaaatc tgttcaagag acaaaggcag ctaagcaagc
    11401 ttccaaaggt gcccaaggta ttcctcaagg agctacattc tctggcgtac aaaaaatcat
    11461 ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc gcttcgatct tgagaaaaaa
    11521 agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa gcaatcagcc aactgacaga
    11581 tgactcgaat acattgatcg ttactcaggt cgaactacaa gaaagagcaa aacaaaaagc
    11641 accgagcgcg cgttttgtct cagtagaaaa ctttttgaat tcacctcgat atgatgaaat
    11701 cattcaagaa ttaagcagca agcagaaaac atcagtttct gaaattgaga atgagccagc
    11761 agaagagttt ctttcagcaa acatcaaaca aatcaaagag attctacttg tccacgatga
    11821 tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa gatatattag acaagaacca
    11881 tttaactatc ccgttaagaa aagtcaatat ccaagattta aaagatgaac ctgattcact
    11941 gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg gctgcacctc aatccattca
    12001 tttatcagtc aacagcatgg tcaatccgca aaaatacgaa tcgattgcac aatatttgaa
    12061 acagacagct taagggagga aaacagcatg caaagtttgc cattcgacat gatcgagctc
    12121 aatcgatctt ttaaaaccaa agaagaagcg atccgctatt gcggtcagat gcttgtagaa
    12181 gcagggtgcg ttgatgagga atatatcgat gccatggtag atagagacaa aatgttatct
    12241 gtttatatgg ggaattttat cgcgattcct catggaacag atgaagcaaa aaaatatgtg
    12301 aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg tagattttgg taccgaagaa
    12361 gaagaaaaga ttgcgactgt tttgttcggt attgcaggag tcggagatga gcatttgcaa
    12421 cttgtacagc aaatcgcact ttattgcagt gatatggaca atgttgtcca gctagcagac
    12481 gcgctcagca aagaagaagt aacggaaaat ctagcaattg cttaaggaga gagaaaaatg
    12541 agagcagtac attttggtgc aggaaatatc ggtcgaggat ttattggaga ggttttggca
    12601 gctaatggat ttgagattac tttcgtagat gtgaatcaca cgatcattga tgctttaaat
    12661 gaaagaaaag aatacgaaat cgaattagca gatgaatcaa aagaaaaaat cactgtaaaa
    12721 aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag taatccaaga aatcgctgaa
    12781 gcagatttag ttactaccgc cattggcccg aatatcttac cttttattgc tgagttgatt
    12841 gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa catctttaga tatcattgcc
    12901 tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag aagtgtataa atatcttccg
    12961 gaaacatcat ttactgacaa atatatcggc tttccaaatg cagcagtcga tcgaatcgtc
    13021 ccattacaac accatgaaga tcctttattc gtccaagtgg aacctttcaa agaatgggtc
    13081 attgacgata gccaaagaaa aaataaaaaa atccaattaa aaggggtttt atatgtagat
    13141 gatctagaac catatatcga acgcaaacta tttagtgtca atactggtca tgcgactgtc
    13201 gcttatacag gtgccttgtt aggttaccag accattgatg aagcaatgca agatgcgtta
    13261 gttgtggcac aattaaaatc agttttgcag gaaacaggaa gtttgctgat tgccaaatgg
    13321 gggtttgatg cagagcagca tcatgcgtat attgaaaaaa tcattcatcg cttccaaaac
    13381 aaaaatatct ctgatgcgat cacacgtgtc gctcggacac cattacgtaa gttaggttat
    13441 caagaaagat ttacacgtcc agtgagagaa ctacaagaac ataacttgac ttgcccacat
    13501 ttgacggcaa caatggggat tatttttaat tactatgatc ctgaagatga acaaagccgt
    13561 caattgcatg aaatgaagat ccatgaaaac ttggaacaat tgattcaaga agtcacagga
    13621 atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg tgaaccgtta tgccaagcaa
    13681 gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa atttttgtca cattcccctt
    13741 tttcgaataa ctaggctgtt tgtccttcaa gatagggcag atccaattct ttaccgtaac
    13801 gttgttctaa agctaggcgg atcaaacgag ctgccaggtt ctcatttctt gcaaggatag
    13861 gtccatggaa ataagaaccg aagacatttc ggtaaatgac accttcagat tggtcttcac
    13921 cattatttcc ttgaccttgt accactttcc ccaaaggtcg ttcaccttca cctaagaaag
    13981 tccgaccatt gtggttttca aatccataat aagtttcatt gaattcttcg ttatggataa
    14041 cgatatcacc gataaaccgg ttattttcct gacttaacgt gtagtgatcc agtgcactga
    14101 ttccttggat cttttctcca ttagctccca tgtagtagtg acctagcaat tggtagccac
    14161 cacaaatggc taacatgacg ccatcatttt cgatatagtt gatcaaagaa tctttctttt
    14221 tctggatgtc ttttgaaatg attaattgct caaagtcttg accaccgcca aagaaaacca
    14281 agtcatattt atcagcatca aaaggttcat gtatactaac gatctccgta tggcaagaaa
    14341 cccccatttt ttctgctata tatttcaaca tcaaaaggtt tccgttatca ccatatgtgt
    14401 tcatcaaatt tccataaagg tgggcgatat tgagttcata attagccacg gttcattcct
    14461 cctttgataa atccttgagc agctagttct ttacgaagct gcaatacagc agtgtaagta
    14521 gctaaaatat agacatgctc agttggcaat tctttgattt tttcgattac ttctggtaat
    14581 tcttttgttt ctgtcaattt attttctgga atcccggcaa cttttagacg taaagtcata
    14641 tctgtatgac ggtctcctcc agaaataaca gctgggatat ccatatcggc aaagttttca
    14701 aaattaccat cccaaatcca gctgacatcg attccgtctg cgtaattggc atttaataaa
    14761 gccactaaag aaaatggata aggagctaga ccaatcatat cgattacttg attgatacct
    14821 acaggatttt tgacaagtac taatgtacat tttttcccat tgatattgat gacttcttgt
    14881 cggccgaata ctttctcgtc atatcctaaa ccttgtttga ttttatcagg atcgacattg
    14941 aagtactctg ccactgcagt agctgctaaa gcattgtaga cattatacat accgccaact
    15001 tcgattccgt attcatgacc atcgatcacg aaatctgctg acacattgtc catccgtacc
    15061 atatcagtta atcgataatc caattcagga cgtttgaagt cacaatttgg acagtaatat
    15121 ttccctaagt ttgcataagt gatcatttta taatgcaaga tgtgatgaca atttggacat
    15181 aagacaccgt ctgtattgta atgagccatt tgttcacgat cttcttcatg gtcaaagcca
    15241 taatattttc taggattgac tgtttcgata gaattgaaga ttggtgaatc accgttactg
    15301 ataatcgttg catttggtga ctttgcggca ccgtctacaa tcattttata ggtcgtatag
    15361 atttctccat accgatccat ttgatcacgg aaaatatttg taaataagaa cagttctggt
    15421 ttcatatatt ctgtgacttt gctcaaactt gcttcatcta tttctaggac agcaaacttt
    15481 ttctgtcctt tttttggttt tgcctgcaag aaagtcgata caattccctg taccatattc
    15541 gcaccagtag ggttggttag aacttcatcg aattgctggc gcaaaatatt gactgttaaa
    15601 gcggtagtca atgttttacc gtttgttccg gtgacaacga cgacttggta gtcttttgcc
    15661 aaactatcta gtatatgtgg atcgactttc agcgctagtt tacctggtaa acttgatccg
    15721 cctttaaaaa aagtttttaa tgcccattgc gacgtttttc caactgcaat cgcaaaatga
    15781 ctgcgtattc ccattatgta acctccaaaa acgatttgct gacgaataaa ttcaactaat
    15841 cataccatat gttgaaaaaa aggttaaatt gcttttctta ttcttaagaa aaggttacgg
    15901 ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac aaaaaaacag cttgaaacaa
    15961 aatgattgtt tcaagctgct gtgattttat tattccctct tacctatatt cccattttaa
    16021 ccgataataa ttttctcgct tggatattca tagcctagat ctcgtgtttc ttttggcacg
    16081 aataacatca atgtatatag cattccgatc cgcccaataa acatcagtgt agcaatcgtg
    16141 attttgccca caacagacaa gtctccggta atccctaaag ataaaccagt tgttccaaac
    16201 gcagaagtta cttcgatgat gatcgagatc agcgtttgtt cttctgtagc agataaaaaa
    16261 acgatacaaa agaaacacat accaagagaa agcataaaga caacgactga ttttcgcaca
    16321 tcatcttgat cgattctccg tccaaaaata ttgatgttat cttcactttt gaggaaggaa
    16381 tatagataca aaccgatgat tgctacagta gttgttcgaa tcccgccgcc tacagaacta
    16441 ggactgcatc cgataaacat cagtaaagaa aaaatgatca gtgttgtgat ttgaaaatca
    16501 cccaaatcgt gaatctgcag accagcatta cgagtcgtaa tcgaataaaa catcgaattg
    16561 atccatttga cactcatatt ggaatcttgg aataaatggt ccttttccaa tagataaatc
    16621 aagacagttc ccgaaacaaa caagataaca aaagctaaca cagctaattt ggtaaacaag
    16681 gaaaaacgaa acggaagctt cgcattggaa cgtttgtata atagccattc ccggcactcc
    16741 atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa acatgatcag gatcaagaaa
    16801 aaatagtcat gtgcaaaggg cttgatggaa tccccagtga cgtcaaaccc agagttcgtg
    16861 acagccgata tcgcctgata aaaaccgtaa aaaacggcat cacgccaacg atcaaaatag
    16921 cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa gtaattgaaa ccagattaaa
    16981 atagcaaaag tgatccgaat cagtcggacg ataccagaca aacgcggttg attcatatcc
    17041 gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt tagaaaaaat gacaaaagca
    17101 gtagaaatca tcatgatccc caaaccacca acttgaaata atatttccag cagtataata
    17161 ccgttatcat tgaacacaga attgatatca aaagtcgata gtccagtcac actcactgta
    17221 ctgattgcca tgaacagcat gtccacaaaa gaaacatggc tatctggttc gcgaaaaatc
    17281 ggcatataaa atagaacgag tgataagacc gtcatcaata tataataaaa aacgatgatt
    17341 tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc gccgctgcag ccttcgaaag
    17401 aataaatcaa catgcacagt actatcctcc tgacaatagt cgtttctacc agtatacaag
    17461 gggaattagg aatactcaaa gaaattatca aacagataac aatacagctt tcgattaaaa
    17521 tagaaatggc ttataataag gaaaatccta gaaaaaagaa ggaggaggaa taacaaattg
    17581 attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa aaataaatta tgatacagtt
    17641 ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa agcctaagat attaatacat
    17701 acatgctgtg ctccttgcag tacgcacaca ttggaattca tgtctcaata tgcagagatc
    17761 acgttatttt tttcaaattc gaatattcat cctaaaagtg agtatttgcg aagattacat
    17821 gaacaaaaac gatttgtaga ccaatttaat gagaaaactg accatcatgt tgcgctaatc
    17881 gttgatgaat accgtccaaa tgaattcacc aaaatggttt taacaaacca tcttgaaaac
    17941 gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata tgcgattaga tcttgttgcg
    18001 caaaaagcgc aagaactagg atacgattac tttggaagtg ccctgaccat ctcgccaaag
    18061 aaaaatgcag ctttgatcaa tcagatcgga atggatatcc aaaagattta cgggacacaa
    18121 tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac gctctttgca aatgtgcaaa
    18181 gagtatgata tttatcgcca atgttattgt ggttgcgtgt tcgctgccaa gcagcaagga
    18241 tgcgatctga aagaaatcaa tcgggaagca aaagcttact taaaaacatc aaccgtagtt
    18301 tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga aaaaaataaa gaatttatgg
    18361 tagaaataag gtagactgta taatttgtcc gtagtaaagt aaggaaacaa gaataaattt
    18421 caatgaagga agagataaga tgcgaaaagt agcacctatt ttgaagccaa tcgctgtatt
    18481 aacaatctcg acgattgtcg cgactactac aggtattgtg aatagttcca gctaccgtgc
    18541 accaagttca agttctagta ctgtagaaga aacaacacca actacttcag tctcgacatc
    18601 tgaaccaaca acaacggaaa gtttgaatga aaccactacg acaagtacat gggacgatac
    18661 aacaactagt tcgtcgtatg atgagacata tacaactgac agctcaagcg aaacaagtaa
    18721 tacggtacca gatggcactc catcggattc aaccaatcca agtacaagtc cggatacaag
    18781 tgctagctcc gaaacgatac caagcagtac gactgtagat gataacacgc aaaatgatga
    18841 tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat acccaaacgt ataaccagca
    18901 agcaggtgat tctcaatgac atttttacta gaccttatca acggggtgca taaattcctt
    18961 ggctatttag acatcagtcc taaatatctt aaccgtggat atacgatttt aagtgtgatt
    19021 cccacgcttt atttattgag aattgtttat ggactttggc aaaaccaaaa ctatcttcaa
    19081 tttttcttat atggacttgc atttctagtg cttgtttatt ttactgtttt aaatgtattt
    19141 tattactttt tagataaaaa cacgaaagcc gatgtcactc agctttttgt gaagtattta
    19201 cctgatgaag cgttcaacat ccagacagaa gaatcgatag ataatggaaa ttcgatcgat
    19261 aaagtgaata cacaagaggt catggtttct tatgacgaag attatcaatt gaaattagcc
    19321 gagaatatgc ggtatttgat tggaaatggt gagatcaaaa caaatgaact cggtcgcatg
    19381 gatggatttc tggtcgatag aaatacattg tatccttatt actatgtcaa gaaaacagga
    19441 gagaaagagt atgttctaaa tattggacga agctactcgg acatggaaaa agtagggacc
    19501 attcgacttg actcaacaga agacacattg gaacctgtcg gattgttcat cgtcggcggt
    19561 gattttgtca aagacgggtt acgctatcat gagccttatc gtctgaaatt gattgcaaaa
    19621 aaggcacagt cgcaggaaga accagtggtt tatagtcgta gccaaagaag aagacattca
    19681 taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa acgtttattc ggaataaggg
    19741 ggatgtgaca cgtcttttgt cacgttcctt ttttatataa cgagaaagat aagacgaaca
    19801 catcacttat tctttccttt catttataaa cattgtagaa taaagaaagt acaacgaagt
    19861 attgtgaaaa aacgtaataa ataacgagga aaatacaagt aggaggtaag ctgtgaaaga
    19921 atttttgaaa aattggggga ttttgatcct tgtattagcg gctattttat tagctagagt
    19981 gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg gaccctacat taagtgatgg
    20041 acaacgattg atttcatcta agatctcaaa ctatgaacga atggatatta ttactacaaa
    20101 ggaaccgggg gatgaagaac ggatgattgt taaacggatc atcggtatgc ctggagatac
    20161 agtcaagatg gaaaatgacc agttgaccat taatggcaaa aagtatgatg aaccttatct
    20221 ggatgagttc aaaaaagaat tttcagaaga taagttacaa ggagaatatg cttatagctc
    20281 aggattccaa gcacaagcag aaagttcatc cacctttacg agtgactttg aatatactgt
    20341 gccaaaagga aagtatcttg ttctaggcga taaccgcttg atttcaaaag acagccgaat
    20401 gtttgggtta gtcgacaaag atatgatcca aggaaaagtt gttttccgtt attggcctct
    20461 ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag ctgtgacatg ttttttgtca
    20521 cagcttcttt ttccgaaaat tccttcttat ttcttggagt tgctcacttt ttttgcaatc
    20581 tcattttaag ctttttggct gatggataag aaaagcgaag cgtttttgtt cataaatagc
    20641 ttttccccac gatggaaggt atgactagta gcagagacaa agcccgtttt ttccaatcga
    20701 ttattcaatt cttcttggct aaagccatta tggactttcg gatgactaat ttgttcattc
    20761 ttgtcaaagt ctacgatgat caattgaccg ccaggagcta aaatttcata aagttttgta
    20821 agaatattct ccgtatcagg aatgtgtaaa agtacaagtg atagaaggat gagattagct
    20881 tggacagcag gaaattcgac agaagcatca gcgtggatcg ttcggacatt tttcagatct
    20941 gctgcttgga tcttttcttc tgccattttc agcatagttt ctgaagcatc ggcaatgatc
    21001 agttctttga atctttccgc aagcggtaaa ctaacaagcc cagttccgcc tccgtaatcc
    21061 agcaggactt tttggtggct gtcgcgcggt agataagtag ttagttcttc gcgaataatc
    21121 ttggctaatg cttgacggtc aggggaatca taatggttag cgatggaatc aaaaatattt
    21181 tttgtcatca aatagctcct tacgtaaaac tttgaaattt taacttatta tagcacgtga
    21241 aaagaaagaa agcatgctat actcaattca aaaagggaga atctagaaag aatatgccga
    21301 tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt ccgagaaata ttactagact
    21361 tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata tatcgaggaa tcaagtaaac
    21421 tacctttatg gtgtgaaaaa agaaaagaag aatatttagg ctttatcact ctttcacaga
    21481 caagtgaaga tactgccgaa atctattcta ttgtatggga ataaaaaaag actatcatca
    21541 ccaaggatta ggaacaaaac tttttaaaga agcagaaggt tacgcagcaa aacattacaa
    21601 atatttgcaa gtgaagactg tagacgaggg gcactactct atatatgatc aaactatttg
    21661 tttctacgaa tcattaggat tttcaaggtt ggaagttttt ccaaatttat gggatgaatg
    21721 gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag tgagcaatag atttaatact
    21781 aatctgtgct tgatgaaaat tctcactgta aaaaatgctg aaaatcaagt ataataccat
    21841 aggaacaaac tggaaggtga aaaagagcat ggcacaacta ttttttaagt atggcgcaat
    21901 gaatagcggc aagacgatcg agatcctaaa agtcgcacat aattatgaag aacaaaataa
    21961 gccagttgtc ttaatgacaa gcggtattga tacaagagat ggtttaggcg ttgtttcaag
    22021 ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa gaaacagacg tatttgacgt
    22081 aattcagaaa atggaaagca aaccctattg tgtgctgatc gatgaagcgc aattcttaaa
    22141 aaagaaacat gtcattgctt ttacgagaat tgtggacgaa ttggatattc cggttatggc
    22201 atttggtttg aaaaatgatt ttcgaaacga gttatttgaa ggatcaaaat atttattgct
    22261 ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg ttctgccaca aaaaagcaat
    22321 catgaattta cattatattg atggaaaccc tgtttatgaa ggcgatcaag tgcaaattgg
    22381 cggaaatgaa gcttattacc ccgtttgtcg aaaacattac tttcacccat caatgattac
    22441 agaaggagaa taagcatgta cgatcaatta caagcaattg aagaccgtta tgaagagctt
    22501 ggagaacttt taagtgaccc agaagtgatt tctgatacaa agcgttttat gcagctttca
    22561 aaagaagaag caaacacgcg cgaaacagtc gaagtttatc gtcgttataa agaagtagta
    22621 caaggaatca ccgatacaga agaactgctt ggtgaaaaac tggatgatga aatggctgaa
    22681 ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag aagtcttaga agaaaaaatc
    22741 aagatcttgc ttttaccaaa agatccaaat gacgataaaa atatcatcat ggaaatccgt
    22801 ggagcagcag gcggagacga agcagcatta ttcgctggcg atcttttcaa tatgtaccaa
    22861 aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg aagctaacgt taccggaatc
    22921 ggtggatata aagaagtgat catgatgatc accggagaca acgtatattc gaaattaaaa
    22981 tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa ctgaatcgca agggcgtatc
    23041 catacttcca ccgcaacagt tgtcgtgatg ccagaagctg aagaagtaga gatcgatatt
    23101 gaagacaaag atatccgtac agatatctat catgcttcag gtgctggcgg acagcacgtc
    23161 aataaaacag catctgctgt acgtttgact cacttaccaa cagggatcgt tgttgctatg
    23221 caagatgaac gttctcaaat caagaaccgt gaaaaagcaa tgaaaatctt gcgtgctcgt
    23281 gtgtacgata agatccagca agaagcacaa agcgagtatg atgccaatcg aaaatcagca
    23341 gtcggaactg gggatcgttc agaacggatc cggacgtata atttcccgca aaatcgtgtg
    23401 actgatcatc gtatcggatt aactatccaa aaattagatc agattttagc agggaaatta
    23461 gatgagatcg ttgatgcatt gatcatgtat gatcaaacat caaaactgga agctatgcaa
    23521 aatgggtaaa atgacctatc gagaagtcct gtcacgggct tcttcttttt tagaggatca
    23581 tgggaaagaa gcatatagta tccaattttt atttttggaa cgaaaacaat ggaaaaaact
    23641 tgattggctc cttcatatga acgaagagat ttcagaagaa gaacagcggt tgatcgaaac
    23701 cgatctgcga cttctgcttg cagatcatcc acctcagtat ttgctaggat atgcagattt
    23761 ttatgatcat cggttaaagg taactgaagc aacactgatc cctcgaccag aaacagagga
    23821 gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg ttagaagtga tagatattgg
    23881 gacagggact ggagcaattg ctattagttt gaaagcagca agaaaaaatt ggcatatatc
    23941 tgctgtggat ctttcagaag aagcattaga agtagcgaaa gaaaatgctc agaaagaagg
    24001 aacgaaaatc agtttttatc atggagatac attaggacca gtaatggatc agcaatttga
    24061 tgtgattatt tctaatccgc cgtacatcag tcggaatgaa tgggaactga tggatgaaag
    24121 tgtccggtca ttcgaaccta aaatggccct ttttgctgag aacgatggat tagctattta
    24181 tgaaaaaata gcagttgaag caccaagcgt actaaaatca aatggaaaga tttttttaga
    24241 aatcggattt cgccaaggtg aagcagtaaa aaaaatattt cagcaagcat ttccagataa
    24301 aaaagtggcg ataaaaaagg atctttttgg caatgaacga atgatccaag tttatggttg
    24361 aagttgtgga tgaaaaattt tttatttgtt tttttattat aaaaaaacac ttatttgaca
    24421 aggatgtaaa cgactttttg gtttatcaac aagtttatcc actattgaca aatagattat
    24481 ccacaaacag tggaaaactt gaggataaac tcagacaaaa taagaatgta tgaaaaaggg
    24541 gttgtggata aactgtggaa acgaaacgat atgatgaaac agaattgcaa gaggcagccg
    24601 aagcactgaa agaaggagaa cttgttgctt ttcctaccga aacagtatac ggattgggcg
    24661 ccaacgctct tttaccaaac gctgtcaaga aagtgttttc tgtaaaaggg agaccgcaag
    24721 ataatccatt aatcgtccat gtcgcctctt ttgaacaagt aaaagagtat gtggacaact
    24781 ttcatccggc agcagagaag attgtgaaga acttctggcc tggaccatta actttgatat
    24841 ttaagatcaa aaaagacact ttgccctcag ttgtgacggg aggattatcc acagctgcat
    24901 ttcgtatacc ggataacaaa aaaacactag aagtaatcga actttccggt gttccgcttg
    24961 tcggtccaag tgcaaatacg tcgggaaaac caagtccaac gactgcagat catgtatatc
    25021 atgatctaca aggaaaaatt accgggatta ttgatgatgg agctacgcga attggtgtag
    25081 aatccactgt tcttgattta agtgatccaa cagccatgcc tatgatttta cgaccaggtg
    25141 cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc tgttgctatt gatcagcatc
    25201 ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa atacaaacac tactcacccg
    25261 atacacgtgt tctgatggtc agagatggcg attggtcaac tgcagttcaa tgggcaaaaa
    25321 ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat tgcggatcaa gtgcggacag
    25381 atactgcagc tgtttatatg tataacgaca actcagtaga agcggcggct aaaggattgt
    25441 ttgcaggtct aagaggatta gatgaaccaa cacttggatt agatttgatt tttgtacaag
    25501 tgtatccaga aactggattg gggaatgctt acatgaatcg cttgaaaaaa gcagcaggac
    25561 aaaattattt tgaaaaatga tcaattggtt attgtcgcat tcttttagta tgatacaatg
    25621 ctatcaagta aatcttctat cagtgaacga aaaactaagc gtaactaatc gtaatagaag
    25681 agatacgaat atttggcaat ttgtttttga tgggaaaaat aaaattgtag gaaaataaaa
    25741 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    25801 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    25861 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    25921 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    25981 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    26041 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    26101 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    26161 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    26221 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    26281 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    26341 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    26401 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    26461 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    26521 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    26581 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    26641 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    26701 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    26761 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    26821 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    26881 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    26941 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    27001 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    27061 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    27121 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    27181 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    27241 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    27301 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    27361 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    27421 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    27481 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    27541 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    27601 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    27661 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    27721 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    27781 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    27841 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    27901 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    27961 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    28021 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    28081 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    28141 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    28201 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    28261 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    28321 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    28381 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    28441 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    28501 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    28561 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    28621 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    28681 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    28741 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    28801 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    28861 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    28921 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    28981 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    29041 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    29101 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    29161 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    29221 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    29281 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    29341 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    29401 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    29461 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    29521 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    29581 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    29641 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    29701 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    29761 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    29821 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    29881 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    29941 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    30001 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    30061 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    30121 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    30181 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    30241 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    30301 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    30361 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    30421 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    30481 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    30541 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    30601 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    30661 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    30721 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    30781 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    30841 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    30901 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    30961 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    31021 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    31081 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    31141 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    31201 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    31261 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    31321 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    31381 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    31441 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    31501 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    31561 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    31621 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    31681 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    31741 gtattttatc ctttaattaa cacaaaaaca gctagtataa
//
LOCUS       DANKMN010000033        31376 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000033 DANKMN010000000
VERSION     DANKMN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..335)
                     /locus_tag="PW224_001392"
     CDS             complement(<1..335)
                     /locus_tag="PW224_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908087.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFA"
     gene            complement(332..1006)
                     /locus_tag="PW224_001393"
     CDS             complement(332..1006)
                     /locus_tag="PW224_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2908088.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2182..3480)
                     /gene="asnS"
                     /locus_tag="PW224_001394"
     CDS             complement(2182..3480)
                     /gene="asnS"
                     /locus_tag="PW224_001394"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL2908089.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(3520..4710)
                     /locus_tag="PW224_001395"
     CDS             complement(3520..4710)
                     /locus_tag="PW224_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2908090.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(4731..5258)
                     /locus_tag="PW224_001396"
     CDS             complement(4731..5258)
                     /locus_tag="PW224_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL2908091.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(5305..8094)
                     /locus_tag="PW224_001397"
     CDS             complement(5305..8094)
                     /locus_tag="PW224_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2908092.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(8241..8441)
                     /locus_tag="PW224_001398"
     CDS             complement(8241..8441)
                     /locus_tag="PW224_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2908093.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(8894..9214)
                     /locus_tag="PW224_001399"
     CDS             complement(8894..9214)
                     /locus_tag="PW224_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908094.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(9717..10835)
                     /locus_tag="PW224_001400"
     CDS             complement(9717..10835)
                     /locus_tag="PW224_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908095.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11306..12613
                     /locus_tag="PW224_001401"
     CDS             11306..12613
                     /locus_tag="PW224_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2908096.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            12734..13933
                     /locus_tag="PW224_001402"
     CDS             12734..13933
                     /locus_tag="PW224_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2908097.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(13960..15228)
                     /locus_tag="PW224_001403"
     CDS             complement(13960..15228)
                     /locus_tag="PW224_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL2908098.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(15398..16039)
                     /locus_tag="PW224_001404"
     CDS             complement(15398..16039)
                     /locus_tag="PW224_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL2908099.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(16448..16951)
                     /locus_tag="PW224_001405"
     CDS             complement(16448..16951)
                     /locus_tag="PW224_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2908100.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17074..17838)
                     /locus_tag="PW224_001406"
     CDS             complement(17074..17838)
                     /locus_tag="PW224_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL2908101.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            17946..18221
                     /locus_tag="PW224_001407"
     CDS             17946..18221
                     /locus_tag="PW224_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL2908102.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            18781..19731
                     /gene="yidC"
                     /locus_tag="PW224_001408"
     CDS             18781..19731
                     /gene="yidC"
                     /locus_tag="PW224_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2908103.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(19777..20178)
                     /locus_tag="PW224_001409"
     CDS             complement(19777..20178)
                     /locus_tag="PW224_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2908104.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20192..21001)
                     /locus_tag="PW224_001410"
     CDS             complement(20192..21001)
                     /locus_tag="PW224_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2908105.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(20988..21878)
                     /locus_tag="PW224_001411"
     CDS             complement(20988..21878)
                     /locus_tag="PW224_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2908106.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(21891..22370)
                     /locus_tag="PW224_001412"
                     /pseudo
     CDS             complement(21891..22370)
                     /locus_tag="PW224_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(22546..23487)
                     /locus_tag="PW224_001413"
     CDS             complement(22546..23487)
                     /locus_tag="PW224_001413"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL2908107.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(23484..24479)
                     /gene="lacD"
                     /locus_tag="PW224_001414"
     CDS             complement(23484..24479)
                     /gene="lacD"
                     /locus_tag="PW224_001414"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2908108.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(24472..25668)
                     /locus_tag="PW224_001415"
     CDS             complement(24472..25668)
                     /locus_tag="PW224_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2908109.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(25686..26414)
                     /locus_tag="PW224_001416"
     CDS             complement(25686..26414)
                     /locus_tag="PW224_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2908110.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(26855..27829)
                     /gene="lacD"
                     /locus_tag="PW224_001417"
     CDS             complement(26855..27829)
                     /gene="lacD"
                     /locus_tag="PW224_001417"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2908111.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(27826..28284)
                     /locus_tag="PW224_001418"
     CDS             complement(27826..28284)
                     /locus_tag="PW224_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2908112.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(28281..29744)
                     /locus_tag="PW224_001419"
     CDS             complement(28281..29744)
                     /locus_tag="PW224_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2908113.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(29726..30658)
                     /gene="pfkB"
                     /locus_tag="PW224_001420"
     CDS             complement(29726..30658)
                     /gene="pfkB"
                     /locus_tag="PW224_001420"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2908114.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            30800..>31376
                     /locus_tag="PW224_001421"
     CDS             30800..>31376
                     /locus_tag="PW224_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2908115.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9135 a         6744 c         5379 g        10118 t
ORIGIN      
        1 tgggcaaaaa ttgattttat tcttcttttt ttagcttcca gcatcaagta ttctgcaaat
       61 tttttttgga tttcttccgt attttctttt gttactaatg tgtgttggat tagttttcct
      121 gatggtcgct gatccacccg ttcttctgac tgttgcacgt ccgcttgcgc aaatccgata
      181 atccgttttt cttttgttac agcaacgagc aatagtttat tcttatcatt taggtcagct
      241 gccgtttttg cttttacgaa tgattctgga tgatcagtaa tcagctgatt gatttgaggt
      301 aaatcttctt ttactgccca ccgtatttcc atcatttctc acttttcttt cgctcatttt
      361 tatctccact aaaaaaatcg actgcggcaa ttgcatctat ccagctttgt gcaatgatct
      421 catgtccgcc aatcgttggg tggaccccat cttctcttgt atatttcgaa aaaccataag
      481 caatccctaa agcattgaat atgccatcta aaggaaccag caatgcgcca tattccttag
      541 caagtttacg aacaatttgg attttagggt ctaagtcttt acgccagcca tgacggtcgt
      601 cggttatagg aagtacaaac ggctccatca agatgatgtt gcagatttcc gctgtttgaa
      661 gttcatttag taattttcga taaacggatt caaattgctg cgcagatttc tcagttccga
      721 atatagcctg atcgctagta ttatgccagg tatcattgat tccgatcaaa atcgatacat
      781 agtcaggttg taaagctaaa caatcttctt gccagcgtac ttgcagatct gcaattttgt
      841 ctcctcctat cccacggttc atgcattcaa atttcatcgt tggatattgg gagattagtt
      901 ttccggcaat caaaagtgga taacctttac ctagatcagt tgcttctgct cggtctcttc
      961 ctgcgtctgt aatgctgtca ccgataaata atatacgttt tttcatttgt caaaaactcc
     1021 tttttgaata aaacgatgat tttcttcctt atattatccc ataagcccta tcaagacgaa
     1081 agagaaaata tatgtataaa aaatgacacc agcctagtcc cttcaatcaa ttgaatgaac
     1141 gctcatcaag tgtctagttt tttatacttt ttttcttaac gtgccggatt ccccttgatt
     1201 ccttcgttgt ttggtccaga gaaatcgatc acacctaagc ttccgtcaat cagaccactg
     1261 atgacgccat cagaacatat atttataggc tcatctaaga cttccagtaa atgttctttt
     1321 actaatatct cttctagatt gcttgggata gattggtcat aactttatct ggtccgatga
     1381 tttcaaggat gaattaaact tacatatgga tcattgcatg ctgaaaatac ggacctactc
     1441 catcttcgaa tgttactatt agtttgtttt caggtactag atgctgcttt atttcaacag
     1501 cagaagcatc atcaaatcag atttccattt gaataacctt ctttgtatat gtttgattga
     1561 ttgcgatttc gttatagaac cttgtgaatc aaatagcaat ttcttttgct ttcttctctt
     1621 gtctgctgga aacttccttt atcccgtatt ttaaaaaaag aactgcgaga gttcgcagtt
     1681 ctttttttta aataaacgtt gctctccggc caactcctcg gtcacaagtc acaatagtca
     1741 tcaaacatga aaaactgttt tttgaaatta ctccttgtga ctcggagctg aacggcaggc
     1801 gcacaacctc ttgatataaa cggtgttcaa tcagctgacc ctcgatatta gctaaaaaaa
     1861 cggtaaaata tgagaagcta tttttccgtt ttgtttctct aatactcagg tcaaggcact
     1921 gatttcacaa cctcttcctt aaacggtgtt caaaagccag tttctcggta ttaagccaaa
     1981 tttatgcaaa aatgtggaaa gccatttcca caaaattttt cttaatactc gaaactaaac
     2041 agctttttca caacctctcc actaaacggt attcaaaagc ttacatctgc ggtaataagt
     2101 cccgccaatt caaaaacatg aacagctgtt tttgcttggc tgttctaatt acttgatgca
     2161 gaacagcttt ttcatcacct cttatggata aattcggttc aacaaacgtg ggaatggaat
     2221 agcttcacgt acatggtcga taccagcaat ccaagtgact gttcgttcaa ggcctagacc
     2281 aaaaccagag tgcggaacag aaccgtattt acgtagatca agataccatg aatattcttt
     2341 ttcatccaaa ccgtgattac ggatttgttc tagcaagaaa tcatagtctg ttgcccgttc
     2401 gcttccgcca ataatttcac cgtaaccttc tggtgcgatc atatctgcac aaattaccac
     2461 gtcttctctt gttggatgtg gtttcatgta gaatggcttg atggcttttg gatagtttag
     2521 gataaagaca ggctgttcga atgaattcgc aatgaacgtt tcatgaggtg aaccaaagtc
     2581 ttcgccccat tcgatgtcat caaatccatt tttcttcagt aattcgattg catcatcgta
     2641 agtaatacga gggaatggca attttgtata agtttttaag acttctttgt ctcgtcctag
     2701 tacatctaat gcatattcgc aatgatctaa aacattttgt actaagaatg ctacatattg
     2761 ttcctgtact tctaaacttt cttcttgatg agtgaaagcc atttctggtt cgatcatcca
     2821 gaattcgatc aaatgacgtc gtgtttttga tttttcagca cggaaagtcg gaccgaatga
     2881 aaagactttt ccgaaagcca tagcagcagc ttccatgtat aattggccac tttgagataa
     2941 ataagctttt tgatcaaagt aatttgtttc gaatagatcc gtagtgcctt ctggtgcaga
     3001 accagtcaag atcggcggat cgattttgac aaaatgatgt tgattaaaga attcatacgt
     3061 tgcacgaatg atttcatttc tgatctgcat aatcgcatgt tgtcttgaag aacgcagcca
     3121 taaatgacga tgatccatca agaaatctgt tccgtgttct tttggtgtga tcggataatc
     3181 gtggctctcg cttaccactt ccacatttgt aattccgatt tcataaccaa attttgaacg
     3241 actgtcttca cggatttctc cagttactaa cacggatgtt tcttggtgta gatttttagc
     3301 tgtttggaaa acttcttccg acacttcgct ttttacaact acaccttgga aaaaggctgt
     3361 tccgtctctt aattgtaaaa atgcgatttt tccgcttgat cgcttatttg cgacccaagc
     3421 gccgattgtt actgtttctc ccacatggtt tttcgaatcg atgatttgaa tttgttccac
     3481 gtatgactct ccttttatat tccttgaatt tcttcaggat taattttgac ttttactctc
     3541 aataaattgc ttgattctgc gtaccgcttc ttctaacgta gcgcgatcag tggcataact
     3601 catgcggaca ttttctggtg ccccaaagcc ttcgccagtc acaagcgcta cccctgtttc
     3661 ttctaaaaga tcttctaccc attgtgtgac attttcatac ttacacatgc gtagcgtttc
     3721 ttttacatta ggaaaaagat agaaagctcc ttgcggcttt tcaagagaaa cacccggcag
     3781 ttctgccaca agcggatata acgtattcag gcgttcttca aaggcgttcc gcatttcttc
     3841 tactgtatct tgctcacctt tcaatgcttc aacagctgca tattgactga ctgctgtcgg
     3901 attgctagtt gattgggaag caacagcgat cattccattg atgatcacag gatttcccac
     3961 cgcataacca atgcgccagc ctgtcatcgc atatgtttta gacacgccgt tgatgatgat
     4021 ggtctgattt ttgattgctt ctgaaatcgt tgcaattgga gtaaactcat tgccattata
     4081 aactaaacgg ccataaatat catcagcaac tatcaaaata tcatttttta ctgcccattc
     4141 accgattgcc tgcagttcat tcttgctata aatcattcca gtcggattcg atggagaatt
     4201 gatgattacg gcttttgttt tttctgaacg tgcttgttct agctgttcaa cagtaacttt
     4261 ccagctattg gattcttccc ctttcacaaa gacaggaagt ccttctgcca gttttacttg
     4321 ctcaccgtaa ctgacccagt aaggcacagg aatgatgact tcatcttgcg ggtcaaggat
     4381 cgtttggaaa agggtataca aagcaaattt cgctccgtct gttacgactg tttgtgaagg
     4441 ttcatagaca agtccatagt ttttcttcag ataatcaacg attgcctgtc gaagctctgg
     4501 gattcctgct gttggtgtat aatagctagc tttcccattg cggattgctt cgattgctgc
     4561 ttcttggatg ttttctggtg tcgcaaaatc cggttctccc acagtcaaac tcagcacatc
     4621 tctgcctttt gcttttaatg ccttggcctt tgccgcagct gccaaagtga cagacggttc
     4681 taatcgtttt gttctttttg ataactccat cccattcatt cctttacctt ttaaattccc
     4741 ttgattgtct tgacttcatc tccagattca aagccaatca ggtaataatt cagtttccca
     4801 tttgtttctt ttcctactac ttcccacaca ggctgatcat catacatccc taaagcagct
     4861 ttttcgattt ggatttcagg atgggccgca gcaattgtct gttttgcttc gttttcagtc
     4921 agtccctttg tttgatccaa tacttttact ttttcaccag atttaggaat gataacggcg
     4981 atttcttttc ctttatcatt tgtacctgtc agtgaaaagt atgtcttctt tcgagtaaac
     5041 caataaaaat gatcggtttc ctcaagattt gcatatttct ttgcgatttc tactgcttct
     5101 ttcttcgcct gcaccgccgg acgactggaa cgcaagtaaa aaatcgagct gatcacgatg
     5161 accgctaata aaaatacgat caagccaagt aacacgcgat taatcgtttt ttcctttgtc
     5221 tgttttttct tcaagggtcg tcgctccttt atcttcattc cttttcatta tagcagattt
     5281 taattatgaa aaaagacagc tcctctaact tccttcttca tttttcttta agaattcatg
     5341 gatatcctga tagatttcct ccatagctag ctctttgatc ggtacttttt tcggtaatgc
     5401 atggatgatc cgtgcgccat attttgcagt gactagtctt cgatccaaaa taagcaatac
     5461 gccacgatct gtctccgtcc ttatcaagcg acctaatgct tggcgtaact tcaaagctgc
     5521 tttaggtacg gattcctggt aaaaaggatt gatcccctct gattctaaat aagcgttcct
     5581 agctttgacc atcgggcgaa gcggattatc gaagggtaaa cgtgtgacta caacgatctg
     5641 caaagcatct ccaggcaaat ctacaccttc ccaaaagctg tcagcgccaa acaatacgct
     5701 tttttctgac aaaatgaagc gtttcaaaag cttctcccgg cttccaccaa acccttgagc
     5761 tagtatttct cgtccttttt ctagcataga cagatgcatt ttctgataga ccttctgcaa
     5821 aacatcatga gaagtgaata acacaagtat tggccgatct tcttgttcga tcagacctgt
     5881 aagtgctgtc tctaaataat cgatatatgc ttgattgctg acttgatgga cggcaggtgc
     5941 ctcttttggc acatagatcc ttgcctgttt ctcataatca taaggagagg caatcacttt
     6001 aagaggtgct gcttcaatgc caagcctctt agagagatag tgtcgattag ggccgatctt
     6061 cagcgttcct ccaatataaa gaatacgatc atatcgagga taccaaacag tcgacggcaa
     6121 catagccgct tgaaaatcat ggagatgaaa aatcatctgc tgcgctgtgt tcccaggaac
     6181 gaaccaatgg atcaaatgat ctcgccagtc ttccagccat tgatccatca gcaagtattg
     6241 cttgtctaat tgatcaaata ggctatataa acttgctaaa tcgatccgca tctggtttgt
     6301 ccagctttct tggataatct caaaagtcag acgcatttgt gactgtagct gctggatctc
     6361 ctgataaaaa agctgcaggt gatcaatggc tgtcccagat tccagtggca gctgatccaa
     6421 catctctttt gtcatcatga cttcttctgg atactgtccg ttaggttgct tgccgctcac
     6481 taaactcatt aatccttctg ccatacaaat ctgtgcttcg catagcgcag aaagttcttc
     6541 tcgataaatc gataaccagc gtaaggcttc atgttctttc ggtaaatagc tttgtattcg
     6601 gtcaaacagc tgatttggtt cttctaattg ctggatttgt ttatggaaat agttaatact
     6661 taatcgtttg tcatttactt tttcagcgat ttccggcaaa tgatgtactt catcgatcaa
     6721 caaaaatgga ctagctggaa gcaatggttc ttttcgctgt gtttcttgta ctaaaaacgc
     6781 atgattgaca atcaaaaaat tactttgcct gattttcttt tgcaagaaac gaacaaagtc
     6841 ttgttcgtaa aatgactgat ttcttgctaa aaatccaata cctctgtgcg cgatatctgc
     6901 aaagaatgaa tggttcaaat ttgttaaatg taattcatcc aaatcaccag tcttcgtttg
     6961 cgtcagccaa actaagaccg ccatttgata caaagcatat tgtttttgtt ctggcggttc
     7021 cacaagtgtg cctttaaaac gctgcaaatc aatatagtgg cgatagcttt tgataattac
     7081 aggttgtatg ggctgatcta gcaattgatt cagcaatgga atgtcttttt ctatgatctg
     7141 ctgctgcaaa actagcgaaa cggtactgat gacagcgggt ttttcaggtg tagccaaata
     7201 actcaatggc agcagataac cgattgtttt acccattcct gtagctgctt cgatcatcaa
     7261 atcttttgat tcatcttttg tgaaatggtc gtagaccgca ttcatcagtc tgttctgttc
     7321 ttttcgatac atcagtgttt taccaaacat tttttctttt gcttgttttt ttcttggata
     7381 tgtccgctcg ccataatgga cggatgtgaa tagttccact tcttttttct gcaaagctag
     7441 cccatcgacg atttctaatg agggatcaag tggctctggt ttttctttca tttcttcaat
     7501 cacatgataa atgaaacgac tcgtatccat acctgtgtgt ctacttaatc gcgcaatctg
     7561 ctctaacgtg atgattggca attttctcat ccgttcttcg atcagcaaaa gaagttgacc
     7621 tgtcacttcg gcatcactat ctgcctgatg aggattctca tgaataaatc caaggctttc
     7681 tgacaaatca gctaagcgaa agcttggttc cgtcggcaag aaaatttgtg caagttctac
     7741 tgtatcgatg cctggtatct tcaaactagg aagtccgcat cgcatgagtt catggttcaa
     7801 aaaattataa tcaaaataaa tattatgcgc gacaaatatg gtgtctgcca aaagattgta
     7861 aatcgtttgg gcgacatctt cgaaataagg cgctttttgg acgcgttgat tcgtgatccc
     7921 tgttaaatgc tggatttgtt ttgaaatctt ccgccctgga tgaatatcga tcgaaaaacg
     7981 tgaagtgatc cgacctgatt caatcatgac gcagccaaat tggatgatcc gatcttcttt
     8041 tggattcgtc ccagtcgttt cgatatcaac gactgcataa gttcttggtt tcaaaatatc
     8101 ttcctcttct cctgcctcta atgttctttt gaaattatac tacaaagtgc agaaaaatac
     8161 tctcatcgct ttttaaagaa aatagacaaa aacgcccaga cacgcaaatg tctgggcgtt
     8221 tattttttag tataggttaa ttaagcttta gtaacgtttg ttgcttgagg tccgcgttgg
     8281 ccttcttcaa catcgaaagt tactgcttgg ccttcttcta aagttttgaa tccgtcgcct
     8341 tggatagctg agaaatgaac gaatacatcg tttccgtttt ctgcagtgat gaatccaaat
     8401 cctttttctg agttaaacca ttttactgta cctgtttcca taataaaaat cctccttgtg
     8461 tatcaacaca tattttgcaa ttacttgaat ggtgtatatc tggaagatgt ttatttgaaa
     8521 caagctacca cagattaccg aacaaaaact actcctatag tatatcatac cgtttacaaa
     8581 aaagctagag tttttcgact ttttttatta aaaaaagttt tctcagcgat tacataaagt
     8641 gaaaaaccga actttttttt aaaataaacg acatattgtt cagttggtgt ttttttgctc
     8701 tgatgttctt ttttaaacta aaaaaattat tctttctgac tacttatcgg ttctgtttca
     8761 acggtcgagg ctggggaaga ttctttagta ggtctgatgt tctcatggta tttctgatag
     8821 cctatgacag ttaccagaag aacaaggcaa accaatacga ccaacgagat tagtttttct
     8881 tttttgctca tatctatacc tcataaataa caggatgatg tccatcatat ttctgagtat
     8941 gagtaaaagc aaaagctgca gctgtagaat gtaagaaatg ccagcaactt tcataatcac
     9001 aggtcggaca gtaccaaaag taaccaaatc ttggctgcaa agaccgttct tctgtttcag
     9061 gaatcgcaga atcaagatct ggtgtctgta cataaacatt tttgtacaga cgtgtgcctg
     9121 taataggaaa ccgctgtgtt tcttcagcaa aaactggctg acttgttaaa cttagggata
     9181 gagacagcaa acaccataac agtctttttt tcataaagac ctccttatcg ataagttttt
     9241 cccaattaaa atcatatctc aagaacgccc gttattcaca ttttgttcat aaatgatgtt
     9301 ttttgtatca taatcgtcaa aaagaggtcg tgaccacgtc acaacctctt ttttaaacgg
     9361 tgttccagaa gtagcttctt cggaaataag ccgaaatttc acaaaaattt gagaaataat
     9421 ttttgtcaat ctcttcttat ttcttgaagc tgaccacttc tgtcacaacc tcttccttaa
     9481 acggtgttca aaagcttgca tctgcggtaa taagcccacc aaacaaaaaa tatgaagagc
     9541 tatttttgct tgctgttctt attacttgat gcagaacagc tttttcacaa cctctcatac
     9601 acggtgttta atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgtgaaact
     9661 atttttccgt ttgtttctct aatactcagg tcaaaacact gatttcacaa cctcttttaa
     9721 taacgaagaa tcgctgtcag actcttttca tctggcgcat ctttttgatc caaaataaac
     9781 acttttccgc cattaatgat cacatcatct gctaatgcat tcaacacttg acgccgatca
     9841 tattctgttt caggatcttc accgaaacca tcagctaagt ttgacgtagc gataaataaa
     9901 tgtgacactt ttccttcttt cgcagctgga acgatatcaa ctaactgatc gatgaatttc
     9961 ttatctaaga gtttattgta ttcttttgat tcttgagctg cgagttcact tgacagcttc
    10021 ttcgcccctt cttcgatatc tctaaaggat agctgcgcag gagaagcaga aatcgcaacg
    10081 tctgcacaat aatagggatt tttagcaatt tttttgaaca tcgtctgatt ttctggtaga
    10141 gcaaataaat aaatcggcca ttgttctgga ttttcgaaag aatccttcaa gtacgtatca
    10201 actgcttggt aatagttgac ccaatcgatc tctacttctt catcttttgg atttacgcca
    10261 tggaaagtta ctccatctcg ggctccgcct cctccattag aagagaagtt caaaccgcca
    10321 cctgtcagtt cagatcccaa tgcagtaacg acatcaaccg gtgccccttc tggtagagat
    10381 acttctttta cttgtttgtt gctgacttca tacaatttca tggagtcgcg gttcaatccc
    10441 aataaataat aagaatagtt gaactgccca tttttgataa tagccagtaa ataaggaaga
    10501 tcacttacat agtattgatc gtctaccggg atactcaaac gatggatata ggtttggtca
    10561 gcagttataa tgaccgctac actcgtagtt gcactacgcc aaaacgaagg atctcctaac
    10621 aaattttcaa ttttttcttg gaacggggac cagtcatgtt cggaatattt tttctcaaaa
    10681 cgtttctttg cttctttcgc aaaatttttg aatttgatct gatctttttc cacatcttga
    10741 tgggcgacat gcgtattcag catgatcgtg acgaatggtc ctttagcgcc ttctgaagtc
    10801 aattgtgaca ataattcttt atcctttttt gccataaacg tacccctcct aaaaaaatca
    10861 ttcctttgtt tctatctaaa gtctaccaca acactatttt tccatacaat aaaacgtatc
    10921 taaaaaaata aaacggtttc tacggtttta aatttagcta tttttttaag ttatcttcat
    10981 aatttcctca attatttagc cccagtcttc aactattctt catgaaatgc tgataaacta
    11041 agaaaaagaa taaaagaggt ggatcttatg catttgatta tgattttcag tttattaagt
    11101 attatcagtt tatttgcttt cagcgcttcc cgcttattag atgcggaaac acagcctatt
    11161 cctgtaaaag tacgtaaaaa cgaccgctaa ccttccataa cgtgcgttct tatcctaaag
    11221 ttttttcaga aacattgaaa ctctctcccc tctctgataa aattttaatt tgtagcgaaa
    11281 caaattaatt acaaggagat cggatatgtc cataaaaaaa acacgtctag tcctattatt
    11341 ggctagtctc attctttgcc ttgcttcttt tctaccagct gttgctgtgc aagctgaaga
    11401 tactttcaaa gtaaatgcaa aagcagcctt tgctgtagat gcgcaaagcg gtaagattct
    11461 ttatgatcaa gatggagaaa aaaccatggg gatcgcttca atcacaaaaa tcatcggtct
    11521 ttacatcgtc cttgatcaag taaaagaagg gaaactatca tgggatgata aagtttcaat
    11581 ttcagattat gcagaaaatt tgagtgtcac cccagattta tcaaatgttc ctttgcataa
    11641 agaaaacacc tacaccgtta aagaattatt cgactctgca atcatccagt cagccaacgc
    11701 ttcaatggtt gccttggcag aaaaaatctc tggcagcgaa gcaaaattca ctgaacgaat
    11761 gaaagaacaa ttgaaagatt ggggtatcaa agatgcaacg atcgttaatg catcaggact
    11821 gaataactct tatcttggcg aaaaccgacc agaaggtact ggagaaaacg acgaaaatca
    11881 aatgtctgct caagatgtgg ctatcgttgc ccgtcatttg atcttggatt ttccagaaat
    11941 actggatgta tctagcacta cgacacaaat gtttggcgaa aacacgcaat caccagtaga
    12001 aatggtcaac tggaactgga tgctcccagg attcgtgaat tataaagaag gcgtggacgg
    12061 attaaaaact ggtacgactg aacttgcagg agcctgtttc gtcggtacga tcactaaaaa
    12121 cgatcaacga atcatcactg tcgtattgaa tgcagacggc catgcggaaa acccaagtgc
    12181 ccgtttcaat gaaacagctc gcttaatgga ttattgctac gacaattggt cagaaaaaga
    12241 gcttggaaaa gccaatgcaa gtatccctag tctaaaaacc attgatgtaa aagacggaaa
    12301 agaatcaaca gtgcccgttg tgcttaaaag tcctgtaaaa gtatgggtac ggaacgatat
    12361 ggatactgga aaattaacaa tcacaccaac tcttgacaaa aaacaagtca ctgacaatga
    12421 gatccaagca cctatggata aaggaacaaa aatcggtacc gcaacgatca ccttggcaga
    12481 agataaatta ggttatcttg aagacaacaa cgcgccatcc acagaaatca tcacggataa
    12541 aaccgtagaa aaagcaaata ttttcgttat tggctggcga cgcgtcgcag aatttttcgg
    12601 taacttgttt taattgaaca aaaagaaaaa agcatctttc catgtggaag atgttttttt
    12661 gctttttcta gtttaaataa cagctttttt catctatttt cgttatactg tataagaaaa
    12721 aggagttgaa tttatgaatc taaccagaga tctgattttt ttggttctct atcttattgg
    12781 ggtattctcc atcgtttatt ggcgaaaaca gaaaaaggat ccttccttgt tcttttacag
    12841 cagtacatgg acgtttgcgg taatcttgac ttattttgtc gtgttggaaa ttttttattc
    12901 gaccttttat tttttgattc ccttagtgtt tttcagtatc tttgcctttt cctatttttc
    12961 agaaaagcga aggctgttga atggtctttt attcaatatt ttcctcatta gttttggtat
    13021 ctacttgttt gtcttgctat acgaaacaca aaatattttt cttggcggac tgattgcctt
    13081 gatcacgatc ccgttactgc ttgtctttct attcggcatt tatggattga tcgtttttct
    13141 tttttggaac ggtgtcacag ttttacgtag agagtcgcat tcattagcta atcttttaac
    13201 gttgatacta gccatttttc ttacattgtt tcttgttttt gactttttct tgttaaaata
    13261 tttaccacaa tggataaatg ctttgttttt ctgcatccca ttgatcttga tctatctatt
    13321 tatcgtattc tacaactttt tgactgtctc attcttgtat cagttcaacc gccctcgata
    13381 caaccaagac ttcatcgttg tgttaggcgc cgggttgatc aatggagaaa cagtttctcc
    13441 tcttcttgcc aagcgaatca ataaggcgat tgctttttat tgggcacaaa gccgtgcgac
    13501 attaaatccg cctatcctat taatgtcggg tggacaggga gcagacgaaa aagtaccaga
    13561 agcaatggcg atgaaacaat atgccatgga acaaggaatt cctgaaagag atattcttgt
    13621 cgaaaccaat tcgacaacca ctttggagaa tatgcgctat tcaaaggaaa tcatggatca
    13681 gcaaatgaaa ggtccctatc gcgcgatctt cagttccaac aattaccaca ttttcagagc
    13741 tggtctctat gcacgacaag cgaaactgaa ggcaaatggc attggggcaa aaacagcttt
    13801 ctattacttg cctaacgcgt ttttaagaga atacatcgcc atcttgatga tgaacaaaaa
    13861 aagacatatg atcatttgcg gattgctttt cgcaggttcc gcattcttat cactgatcac
    13921 tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt ttttttcttt
    13981 tattctcttg acgattggca agaatagtat tccttttttg acggctactt gctgtgcaga
    14041 ataaataccg ctgtttcctg aacacataaa gctgatcaag cagatcataa agaagtaagc
    14101 agcagcttgt gaaccaaata attcgattcc catcacaaaa caggcaatcg gtgtatttgt
    14161 cgcaccagaa aataccccga taaaacctaa tccagcaaga aaaggaacag aaacatggag
    14221 caaaggcgct agtactgcgc caagcgttgc accaatctca aatagtggtg tcacttctcc
    14281 cccttggtac cccgcaccta aagacaagac cgtgaagaat aatttcccaa taaaatcata
    14341 agcatgtgcc tgtccattaa atgattcctg cagtaacgga agactcaacc caaggtaacg
    14401 ttgtgtttgc aaaatcaacg ctgcggctac aactactgct ccacctatcg tatttcgcca
    14461 aacaggatga ggaatccatt gcgcataccg cgcttttaaa aagacgatcg aacggctgaa
    14521 tacccaacca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga acagcaacat
    14581 actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat agctttctgt
    14641 catgaaattt gcaaaaagcg cagcaaagaa gctaggaaat attgcttctg ttcggacttt
    14701 accgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc caaatacaga
    14761 actaaaaccc gcactgattc cactgataac caaaatctcc cgttctgctt tatccaatcg
    14821 gaataaatgc gctatattat cagctaatgc tccccccatt tgaactgctg ttccttcacg
    14881 accaacagat cccccaaata aatgggtagt gatggtccca aacaaggtca atggaatgag
    14941 ccgcaaaggg atcttctctt ctcctccatt cccttgatcg atcactaagt tattcccacg
    15001 actagaaaga ccgccatgat acgcataaag ataagcaaaa agcgcgccgc tgactggcaa
    15061 tagataaagt agccaaggat gtgctagtcg gatatctgtc acgaacgtca ggctatttaa
    15121 gaaaaaagca gacaagctcc ccatgaatac tccgagaatc gctgtgacca ccagccactt
    15181 caacatgtac atcaaaatac gcactggact cggtaattgt ttttccataa aaaattcctc
    15241 ctcaaataaa aaaacgccta ctaaagaaag gggctggccc cagactttag taggcgtcat
    15301 tagctatctt acgttagcat ttaaaggcga acaccatcac ctattcactt ccattaaaag
    15361 atagcatatt tttctgtaaa aaacaataaa attatcgtta tttctttaca cgttcagcaa
    15421 attcatagaa aggagccaat ttcgttaact gttgattaca ttgatcacta tcatgacaaa
    15481 tataattccc ctttttcgta tacgtgccat cagaaccggc tttcgttgta gaaagaaaga
    15541 gcgagacatt cgatgttttc tgacagatcg cacagattcc cttattaatc gttggtgata
    15601 aagtaccgct gatccctttt aattttccat cttcgtaata aaccatgaat tttttttgcg
    15661 tccctggatc attccaaccg atgtacgtat gtgcttgcaa gtcgatttca ttccaagcgg
    15721 gttgtttgag ttttttcact tttcggaata atttctcgat ttgtggtttg ctcggctgca
    15781 taaacggctg gacgatttgc ttcagctcct ccaatgctcg ttctgcttgc gtttttgtca
    15841 gttttacatc taaaactgtc gttaaaaacg tatcgatttc tgatggttcg taacctaata
    15901 catcgcgaat cttttcagct gctagagcct gaaccgtttg taccgttttt gagtcattga
    15961 cagatgagta cgcatttaat aactgctcta cttctctttt tataaaaaaa tattgatatg
    16021 gtttcattgt ttgttccatg attttaaagt cctttcttct aaccaaagtg catactttgt
    16081 agaaaaaagt gatcttctgc aaagtatgtg ttacctgaag atcccggact cttttaaata
    16141 agatacgttt tgcatcttat catcaccccc tttaatgttc attcttctat gtacatgatt
    16201 cattcgataa ataaaagtaa accgtatctt gagcagatag tcaactgcaa acagaaaaag
    16261 gactgtgaca aaagtcgtgt caaagtccct ttctacaaac aaacggtgtt ccagaagtag
    16321 cttcttcaaa aaataagccg gaatttcaca aaaatttgag aagcaatttt cgtaaattcc
    16381 ttcttatttc ttgaagctga cccacttctg tcacaacctc aatatggtct attattggtt
    16441 ttacctgtta aatcgaggat tcttttccga agtaacgttg ccatctctta cgcatacttg
    16501 ctgacatagg ctgagcggct tctttgactg cgcagtattt gtctacgaat gttacgatat
    16561 atccttcttt gtacttaggc ggtgcaatcg ttgcgcccca catgtgcttc aaaatgatat
    16621 ctcgctcaag atccgacaaa tcggtgatct tttctgcatt ctttacagcg attcttggat
    16681 ggatatacgc atgtgtgcct tcatcaaatt tcgtcgtacg ccaatcatag tagaacagat
    16741 catgcaaaag tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca
    16801 gataactata gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg
    16861 tgtaattcgc taattttttc actgcttctg tttctaaaag atcttctaca taagacatat
    16921 attcttcgtc ttcacgccat ttttctgtca taattatcga tctgccacct ttttgaattt
    16981 cattttcttc tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgcctt
    17041 ggataaaata gccaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacaat
    17101 ccctgccgca accccaacat tcaacgattc cgcatttcct ttcatcggga tatatacgtt
    17161 ctgatcagtc aaagacaaga tttcctgact taccccctga ccttcgttcc ccatgattat
    17221 tgcgaagtta tctgattttt ctactttgtc taaggcgtgt gcttcttcat ttaattctgt
    17281 gccatacact gtaaaccctt tgtctttcat taaaggaatc acttctgtca aatctttttg
    17341 caacacaggc aaatgataat tgcttccctg catgctgcgc agcacctttg tactataaat
    17401 atctgccgtt cctttgccta ataaaacacc agccatatca aatgcatctg ccgtacggat
    17461 catcgtccct acatttccag gatcttgtac attatctaat aacagccatc ctcctgaaaa
    17521 atctggcgtg ccttcagccg ttaacgccat cactgctaag attccttgtg gtgtaggtag
    17581 atccgataca gcatccatga cctcatccga aactagaaaa gtagaaatct ctttgtttgc
    17641 ggccagccag tctccccatt ctttcatgcc tcgctgtgtc acaaaaagtt cttttatttc
    17701 tgcatcagac gacgcagctt cttcaatcag atggaaccct tccaaaagat attcttcctt
    17761 ttcttcccga tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttgc
    17821 tgattggatc tctttcacat tcctcacctc cccatgatta tatcatggaa taggtgcttg
    17881 agcttgtttc atcctaaaaa aatcggtatc attaatttat aaaataggaa agaaaggtga
    17941 cgaacatgcg caaagttaaa atgaacgtcc aaggacgcgt ccaaggcgta ggatttcgct
    18001 acatgaccaa gatggtcgct gatcaactag gcgtgactgg aacggtaaaa aacgaagatg
    18061 acggctcagt cacgatcgaa gcaatcggca atgacgacat catccagaaa tttattgaag
    18121 aagtcaagaa atctccgagc ccaagtggac gagtgcagta tgtggatgtc caagaagacc
    18181 cgatgattga ggaacgaaag aaatttgatg tggtgggata aggttgtgaa aaagctggtc
    18241 tgcatcaagc attaaggaca ggttgtgaaa aatagctctc catatttttc aacaggctgg
    18301 acttaatgcc gtagatgcaa gcttttgaac accgtcaata aggttgtgag aaagtcgctt
    18361 agttttgagt agtaaggaga attttgtgaa aatggctttc cacatttttg cataaaattt
    18421 gacttaatac cgagaaactg acttttgaac accgtcgata aggttgtttt tgagacgctt
    18481 tgcctcaagt attaaagata ggtagtaaaa aagctgatct ataacttgca ataaatttgc
    18541 ttaacccata ttttccagac agaacaaaaa agtttttagt tttttaaata agaaagaaac
    18601 acttggaatc actcttttct ttccaagtat ttctttcttg attttttgtc tttttttcaa
    18661 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
    18721 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
    18781 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
    18841 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
    18901 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgagaattt caattggtct
    18961 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
    19021 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
    19081 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
    19141 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
    19201 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
    19261 acagaaggta ttcgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
    19321 cttgttgtca ttgcaggggt cgcctatctg cttcaaggat acatctcaac gatcggtatt
    19381 cctgaagaac agaaaaagac gatgcgtacg atgctaatcg tctctcctgc tatgatcgtc
    19441 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    19501 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    19561 gcagaagagt tgaaacaaaa cccgccaaaa caagtcgtca ctccaagaaa agacgtgaca
    19621 gaatcagcaa agccagctaa caacaaacaa gcaacaaaac aaatcaaaca accaaaaaac
    19681 agtacaaaca acaaaggccg caacgcagga aagcagcaga agcggaaata aaaacatagg
    19741 acaaaaaaga tgagagctaa gccctcatct tttttgttac tcttcctctt ctgctattcc
    19801 attaattaat tcgttgacag agacaatgct ttcacatcct gcacgaacaa actcgggctc
    19861 tgaaccaatc atttgtgagt tgataaattc aatgaccatc ggaagattca tcccagcata
    19921 aagagtaatc tgttgtcctt ccattattag tttagaaatg atatttgcag gtgtaccacc
    19981 taaaagatcg catagtacca caaaatcatt taacgaatct gtgacagtga gaaacttttg
    20041 tttgtatgtt tcagtgccat cttctggtaa taaaggtaac gtgtagatat cctcttgagg
    20101 ccccatgatc atttctgtac tttttttcaa ttcctcacaa aattttccgt gactaatcaa
    20161 taccaattgt ctactcattt ttcgctctcc cttatttcga aatcacaccc aatgcagata
    20221 aagcaacaca cacaattaat acgataaaaa tcgctcgagt aggagtcatg tttttctttc
    20281 ctaataacca ataaacacca ccgacgatcc cagcaggtaa aattttaggt aagatcatat
    20341 cgatatagtt ttgcatattc aatgtcacat cgccaatcgt tggtgcccat gaaaatttaa
    20401 cattgatcat tgtcgcaact aatgccccta ccatgaatac ccctaataaa gtggctgcgt
    20461 ccgttaatgc agttaagcga gattgcatag tagtaactaa ggaaattcct tctttgtatg
    20521 caaattcaag ttgcttccaa cgaaaaacca tgacaacgat ctgagcaaaa atccatataa
    20581 acacacctat cggatttcca tcagttgcca tattcgccgc caaagcccca aaaatcgttg
    20641 gaatcaaagc agcaaatacg gaatctccaa tcgctgcaaa ggaacccatc aatccagctt
    20701 tcagccccga tacggtttcc tttgaagcaa ttccctcttt ttcttccatt gcaagatcaa
    20761 tcccagtaat gattgtatta aaaaaattag aagtattaaa aaattgcgta tgtgttttca
    20821 tcatctcttt taattctggt gtattgtctc catatatttt tcttagttgt ggcaagatag
    20881 tatataaata gccagaaccc tgcattctct catagttcca acctaattga aaagtgaaca
    20941 aacttcttcg attgatttgc ttaaaatctt cttttgttaa tttgtaatta gatttcgtca
    21001 tcttcgattt ccccctcaac agaatgtgat ggctcactag ctaatggttg atggtctttg
    21061 ttttgatcat tcttataact gatcattgct agtgctagac caatcaatgc tacagcaagc
    21121 atcggcagcg aattgaaagt tgccggaaga tctttaacaa cgcttgccac acttgttcct
    21181 aataattgaa cattagagaa taacgttgct aataatgccg tgatcgtaaa tcctaaaata
    21241 agataagata gatgtttttt tactggtaaa tagcgcagaa gaatggcaaa cccaacagct
    21301 ggtaaaacag ctcctgctac tgaaagtcca tcgcctagcc attttagatt cccgtttaaa
    21361 acagaaacaa cattttcaac taatccacca ccaaatgcta aagctaaaaa tacaggaatc
    21421 atacgagaga gcgaccaagg taaagctccc attaagaaat tgcgttcgat tgcttgataa
    21481 ttcatgtctt ctacgtgacg gtcaatacga tgggcaaagt atgtgttagc gaatctagct
    21541 aaaatatcca attggatcat taagcttgct acaggcactg caatagcagc aatcgcttgt
    21601 tctggattca ttccaagaga aatcgagaag gcagttgcta aaatcgtccc agagttggca
    21661 tctatttttg atgcgcctcc aaacgtcccc actcctaaaa ctgtcaactg catactgcca
    21721 ccaataatca aacctgcgtt taaatcaccc attactaaac ctgcatataa ccctgcaaac
    21781 actggcgcac tcattgaaga atagatctgt aattcatcta aaatttgata acctgcatat
    21841 aatgttaata ataagatctg ccaccattga atatccatgg atcattcctc ctatttgatt
    21901 aaaagattca taaagtcttc tgctttatcg tttggtacca tttgagcaat taaatgtata
    21961 ccaagctgat ctaatttttt aaatacttcg atatctgcat ctaccacatt gattgatttt
    22021 gtaatggacc gtgtttcatt cgtttgtgac atattcccca catttacttc tttgatagga
    22081 actccttttt ctactaaaga taagattcga tctggctttt ttgctacgat caatactcgt
    22141 tgagagtcat atttgccagc taaaatattt gttgctgctt tttcgattgg caaaatgctt
    22201 aattttaccc ctgctggagt cgctaacttc aatccacttt tttcaattgt gtttgtggct
    22261 acctcatcat ccacgaccat gattcgacta atattcagtt tcgttgtcca aagatttgct
    22321 acttatccat gtatcaaacg tccatcgatt cttacaccaa taattgccat catgcattct
    22381 ccttatctat tttttctatt tattgttcaa ttaaaactgt gacatcatca aatatccctt
    22441 ctgtttcaaa gataacgttt tcattttttc tttcttatat ttcattttta agctaagaac
    22501 aaattccttc ccaactaaag aaaacgcttt cttttatatt taagttcatt tgattttctt
    22561 aacttgaatg tgtttcatca acatcgtaac tttcataggg tcgatccagc cagttttctc
    22621 ttgttgcgcg tttgccatac cacaagccat agaaaatgcg agaatctctt cgattgattg
    22681 cctttttgat aacgcatggg ccgatcctgc aagggtcgcg tcacctgaac caacaggatt
    22741 aacaactgat attttaggaa taatgacttc gtaaaaacac tgagcaactt ttgccacagc
    22801 acctctctct cctaaagaaa caacaaccca tggcactcct tcaaatattg gatgagctaa
    22861 taaggactga attttttcta attgaatcga agaacagata cgttggccaa tcagttctcc
    22921 taattcttcc agattgggct tgataagtag tggcttctcc ttaccttcca atacttcaag
    22981 caaaaattta ccagaagtat ctagtaatat gtctttccct tttttatcag ctaaagcaac
    23041 caattgctta tagtaatcaa gagggcatcc ttggggtaaa ctacctgaga tcgtcataat
    23101 atctgatatt tcgaggagct gctcgaaatg atcggtaaat gtctgttgtt ctttctttgt
    23161 tatgaagggg cctcgttcca aaagctctgt ttgtctcgaa ctatgcagga tagcaataga
    23221 atcccttgtc tctcctttaa ttggaacaaa ctgatgcatg atccctaact gatctagctg
    23281 gttctttaga tattcaccgt gctggccacc tattaaacca gtagctatca cctcttctcc
    23341 taataaattt agaaccctcg ccacattcac tccttttcct cccgctgttt tattgactgt
    23401 ttcaactcga ttgactttgt cgaggtttaa ttcttcaatt gtatacgcga tatcgatgga
    23461 aggattcaag gtaactgtcg taatcatttc atcttcacct ttttgctttt tgttttattg
    23521 aaccaagggg tagcggtttc atttattact ttatttaaag tctcgatgtt ttctctccca
    23581 tttgttctta accaatgaat agctccgtct tttccatttt cgacgtatgc tttgacccca
    23641 tctttccatg tcgcacgtcc acacaataca ccattgaagg tggaacccga ttcatgagca
    23701 aaaatcagtg tttgttgaaa taattctgtt gaaacacccc cactcaaaaa aatgaatggt
    23761 aatggagtcg cttcactttg ctccttaaag aaggcagcag cctcttcttt gctgtgaata
    23821 aattcacctg tggaatagcc attcacataa ttcatattga ctggtacttc tactttcaac
    23881 acatcaactt tatattttgg atcagaaaat tctttcatca tctcaataac tttgtggggc
    23941 ttgactttcg catactctgg agaagaacta tcgcttattt gtgcgtcata agaaactaac
    24001 tccaaataga agggacgatt ttctccttcg cactcactac ctaaacgttc gatgaacaca
    24061 tgcttcaaat gattgatttc gggtgcttca tcaacatcgt agtaaagtaa gaattttact
    24121 gcatccgctt gctgttcttt caatctgaga gaagaccaat ccgctaataa atcaggtaga
    24181 cgaccaggcg tagttgcgtc atagccggtt ttctcataag ctaacaataa ccccgtctct
    24241 tttgctctga cttttgctgc tggcaatcca tattccggat ccaataaaat agcagaagca
    24301 tagggtgtca gctcagctga tactagttct ttgaattcac tgatttggtc ttcacttggc
    24361 tcttttccca acgatttgat catttttttt agagcaccac gctgatcgat cgctaatgca
    24421 ctaataatcc catcttttgt tgatagtctc tccattgccc tatttttttc ttcattcatc
    24481 tgtcatttct ccttcggttt catacttatg aataatgacg cccttgacta ctcgattgac
    24541 cgtgcctgtt ggagatggtg tatctggttt attagctact ttgattgaag tatagagtgc
    24601 aatgatttgt gcagtcataa tcatcgctaa tgcaagatag gcatcgggca atttactcac
    24661 cgagtcttcc aatagcacag tcatacctga gaaatcaata gtacccggtt gcgcaatcgc
    24721 aatgacattg gcagtgaccc gatcttcttt tatttcctct aaaatatcta agtcataatt
    24781 tcgcgtatat ggttgattgc tcacaaaacc tacaacgagt gtctcgctat ttaaaaaaga
    24841 ctttggtcca tgacgaaacc ccatcgaaga atcaaaaatt gtagcaactt tacctgctgt
    24901 taattctaaa atttttaatt gtgcttctct tgtcgcacct gccaaacttc ctgaacccag
    24961 ataagcgatc cgttcaaaaa aatcaggtaa taacttttga atttcttgat ctcttgaaag
    25021 aagatcttga ccgattttaa ctaattgttc aagatatctt tcttttttct ccattgctgt
    25081 ttgatcaaag atccataaag cactcaataa catacacgta aaacttcctg tcatagcaaa
    25141 acctgcatcg tttgctctct ctggcatcaa taacagataa ttctgtttat catcctgtgc
    25201 gttttttgcc aacatcccct ctttagcaca agtaatcgtc aaatgaaaca atgtttcaac
    25261 ttgttcattt gcaatttgca cagccgctac actttctgga ctgttgccac tgcgagcaaa
    25321 tgaaactaat aaagtcggtt catttggaaa caaaaattct tttggtgaag ctacaatatc
    25381 tgtcgtgcca aaactgtgga agataaaaaa ctgattgtct ccatgttcat ttagataggg
    25441 taagagcgta tccccaatat attgagacgt tcctgcaccc gtaaaaataa cacgtattct
    25501 tcttccgttt gcttcttttt ttatttgatc aaaaaataga gctaaatttt cttggctttg
    25561 catcaatcta tcgattgttt ctttccagag atctggttgt tgttttattt cttgtgtcgt
    25621 gattgctgca cctatttgtt ttaatttgtt cttcgatagc tcaaacatgt tgttctccct
    25681 ctcaatcatt ttcggatatg ccgaatttta taatgaaact ggtccgctct tgctacacta
    25741 tctgtatatt cgataatctc atttttcacg ttataggtta tcctcgtcag atgtaagacg
    25801 ggagcgccat cagcgattcg tagcacaaca gcatcttttt tttgcacgat acttgcataa
    25861 aactcttcat cagctaattg aacttgttgt tcataatcct gtgaaaacac atcatacaac
    25921 ggtttacttt caagtaacgg caaatcaagt cccataaata gtttcgccgg tagataagaa
    25981 cgctcaacca tcaatggctc ttcatctgcc aaacgcaatc ttttaagttt aaatacagtt
    26041 tcacctaaat ggatgttcaa attcttagca attctcttat tagcttccat ttgtctaaat
    26101 tctaaaatga ccgtcttagg gacgcgccct atttcgcgca tttcttctgt aaagctatat
    26161 gctccagcta agttcgatga cttatcagac aaatcagaga cataggtacc ttttccatgt
    26221 cttcggtaaa tataaccttg attttccaat tcctgtaaag ccagacgtac ggtggtacga
    26281 ctcaccccat agtgttgaca aagttctctt tcagataaca gcttatcatg cggagtcatc
    26341 tgtgtttcga tacgatcctt caataaatca actagttgat aatacaatga ttttccttta
    26401 tttgagctct ccataaaaat cacctcttct ttactggtta taaccacaac acaagaatag
    26461 tgaattatta atcatttgtc aaaggttttt tagaccaaaa aagctattat aacagctctt
    26521 taaagcttta tatcttgaat agacaaagat acctcaaaaa taccagttac caataaaaga
    26581 acagaaaaaa agacgaacca cataagcgat ccgtcaaaat ggtctaactt atcgagcagc
    26641 gtgatccaca ttaatcccta tgatattcct acaactatcg ttttattact tccaatatca
    26701 tttacaacaa ctaaaaatag cttattaatg acaaatgtta ttattagttt tagataactt
    26761 ttgcagtttt atactttttt tctataaaat ttgacaaaca tttttttaaa taactaatta
    26821 ttttttgaat ctgtcactca gacaatcctt tctcttactt gttaacataa atagaagttg
    26881 ctgtttcttc taatagtgta cttaattctt taatattttg ttttccttct tctaacatcc
    26941 aactactcgc tttttcttcg ccttcggtaa caaaaggtgc tactcccttc gcccatgttg
    27001 ctcgcccaca taagacacca ttgaatgtcg aacctgattg ttttgcaaaa cgcaatgtat
    27061 cttgaaatag tgcagcgctg actcccgcac ttaaaaaaat aaacggttgt tcagtcactt
    27121 tcgattgttc atagaaatac gcggatgcct ctgcttgact ataacaagta tcttgcccat
    27181 atccttccac aaatctcata ttcactggta cttctacttt caatacatcc acttgataac
    27241 gttcttttga aaattctcgc atggcttcaa tcactttatg cggttttact tttgcatagt
    27301 caagagaaga cggatcgcca atagttgcat cgtaagaaag gagctctaaa aagaaaggta
    27361 tgccttctgc ttcgcattct gaaccaatcc gttctataac tatttgtttt tgcacattaa
    27421 tctctactgg ttcatcaacg tcatagtaca ataaaaattt acaagcatcc gcacccactt
    27481 cttttaacct tttcactgac caactattca gtaaatctgg tagtcggcct ggcattttcg
    27541 catcatagcc ggttttttca taagctagca acaaaccaca ttctttctct cgaagcgctg
    27601 ctgcaggcag accatattct ggatcgagta aaatagcgga agcatacgga gtcaggtact
    27661 gagaaaccat ctgtttaaat tggatgatat cttctgatgt agcttcttta tattggttga
    27721 tcattttctt caaagcacca cgttggtcga tagctagcgc gctaataatt ccagcttcat
    27781 ccgtcaatcg cttcaaacgt tcctgttttt ctatactcat tttcttcata tatcttcacc
    27841 tatttcttta gttaatattg tagctatttc tatttttgat tttgcagctc tcaactgttc
    27901 tgtaacctct tttcgcatca atgatcgtgc aacgagagac aataatttca aatgagtcgt
    27961 tcctgcttct gaatctggga taaataacgc aaaaataaaa tcaactgact gtctatctaa
    28021 actgttccat tcaattcctt ttttcagtgt gacgataatc aaactaggtt cattgattgt
    28081 ttgagacttc gcatgaggga tagcaaaacc tgacattatt cctgtagtac cttctttttc
    28141 tctttccaga agtttttgat atacttcttt ttcgtcatga gcgattccta aacagaatgt
    28201 ttgctttgct aaaaacttaa atgcttcttc ttgattatct aatggctgtt gtaaaaaaat
    28261 gttctcttca tgaatgattg tcatgataaa cctcctgctc ttccttcttc gataactgta
    28321 cgatttgttg ctttattatc cctcttcttg tctttatcag ttactctttt cttttggatc
    28381 aaacctagta tcactccacc aacgatcgaa ccaataagaa tcgctgtaac ccattggatt
    28441 tttcccgtca ctacaggtaa cactaaaaaa ccgccatgtg gggctggtac ttggacacca
    28501 aacagatagg tcaagacagc agcaattgat gagcctaaca tcatcgttgg taatactcgg
    28561 atcgggtctt tcgcagcaaa cggaatagct ccttccgtaa tatgagttga tcctaaaata
    28621 aaattaacca aacctgcatt acgatcatta gtatcaaaat atttaccaaa caataaagta
    28681 gcaaaaccag taatcaacgg tggtgcaatg caagctgctg acgcaccagc cataaaagtc
    28741 gtgtttcctt gtgtcaaaag agctgttcct gttacatatg ccgccttatt aaccggtcct
    28801 cccatatcaa acgcacacat acagccaaca atcacgccaa gaacaagtgg attcgaatat
    28861 tcaaatccag ctaaaaagct catcattcct tcatttatag ccttcattgg acctgccagc
    28921 aaagacatca cgatgccaat aaatgctaca ccaataactg gatataagac gattgccttc
    28981 aagccatcta gttgtttagg taaaattatc agtagctttt gcaaaaataa gataaaataa
    29041 cctgctaaga acccagctaa aatacctcct aaaaatccag taccgccatt catggctacg
    29101 attccaccaa caaatccaac gactaaccct ggacgtttcg cgatcccttc tgcaatataa
    29161 gctgctaaaa ctggaaccat cattcccatt gctgcatcgt caactgcttt taccattgcc
    29221 gctgtagcat tgtattgtgc atgatctgga tcagaagaat agattcccca aacggcaaat
    29281 gaaatagcaa tcaatactcc gccagctacc acgaaaggca acatatgaga gacaccgttc
    29341 atcaaatttt tatagatgct acgaccaaaa ttagcaccac ttatcgcttc tggttctaaa
    29401 gtatcagcag cttcattatc tgctaagaca gtaccttttc ctgccaatgc ttcgtcaatc
    29461 agccgttgtg catccttgat tcctgtactg acagaaacat caatgactcg ttttcccaca
    29521 aatctttctg aatggacatc tttgtctgcg gcaataatga cagcatctgc ctgtttgatt
    29581 tcttcagccg ttaattcatt ctcgatccca atttgtccat gggtctccac tttgatcgtg
    29641 atgccttttt tcctagctgc ttgctctaat gcctcttgcg ccatgtaggt gtgcgcaatt
    29701 cctgtcggac aaccggttgc tgcaattagt tgatattttc ccatctttcg aacctctctt
    29761 ccttgatttt gatttgttta cttaattcct ccacatccaa aaaatcagtc agtcctttac
    29821 gaaaagcagt agagctgcct gctgctatgc ttttacgtaa cgtttcatca agcgatcgtc
    29881 gattcatgta tcctgctaaa aaagtgccta acatggcatc acctgcacat gctgtattga
    29941 caacttttcc ttttggcgag tttcctcgaa tcaccctgtc tttcgtaaaa agaatcgctc
    30001 ctttctcacc gagggataac aaaacatttt ctgcgcctga ctctactagt cgttgtccat
    30061 aaacaaagta atcttcatct gtaaccattt ttcgtccgaa ccaagaagaa agttcttcct
    30121 catttggttt cagcaaaaac ggtcgatagg gtaaacaatc catgacttct tgatcactac
    30181 tatcaataat caaacaaatc cgactgattt ctattaaaac ctttggagac agccctcttg
    30241 gtaaactccc agaaacacaa agataatccc ctgcttgtaa tgctcttatt tgatttaaaa
    30301 aattatgaac agctgtctgc ggtatttcag gtccttgatt cactaatttg tattcttctc
    30361 ccgtttgatt gacttgtgta aatacattga tacgcgtcat accaggcact tcaataaact
    30421 ctgtttggat ttgtttctgc tgtaaatagt cttctatata ctttcctgtg aacccagcag
    30481 aaaaacctaa tgccgtactt gggaaatcta gcatctttaa tactagtgac acattcaccc
    30541 cttttccgtt tgcctgaatg tcatcctctt ttgtgcggtt taccataaaa ggatgcatct
    30601 catctgtttc aataaataga tcaattgcca aattcatcgt gcatgtgtag atagacataa
    30661 ccaacgacct ccttgattct attatgaaat acgcgaaata gaaaatgttt tcttaatcgt
    30721 cccaacacaa agaaaacctt tacatttata aactcgtcgt gcttttttta actaactttt
    30781 ggtgaaagaa ggaaagaata tgaatcacaa attaagtgaa acagagcagt acctctggaa
    30841 ctttatcgaa cacctcatcc tagagatccc taactattcc atcgtgaaac taagtgaaca
    30901 agcaaatgtc tcaacagcca ccattgtgcg gacaatgaaa aagaaaggct atgaagggtt
    30961 tacttcgttt aagcatcatc taaaagaagg agaaaacaag aacatcaatt tttcattttt
    31021 agataaagta gacaaaggaa tccgccgagc cattctaaaa aatgaacaag aagtggtacg
    31081 tacaatcaat atgttggaaa tcgggaacat tgaagatgca atacaaagaa tcaaatttgc
    31141 tgaccgggtt tttgtctttg cccgaggatt ttcagaaatg attggccaag aaatgctggt
    31201 aaaacttcaa ttaacgggaa aaaattgtca gatgcatact gatccagaaa ttatcaaaag
    31261 catcagtcgg aaactaacta agaaagacag cgttattttt gtttctttga acggtgagaa
    31321 aacaaataaa aacatcaaaa ataagattta tgtctttatt tttcaatttt ttaaat
//
LOCUS       DANKMN010000034        31014 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000034 DANKMN010000000
VERSION     DANKMN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(405..2480)
                     /locus_tag="PW224_001422"
     CDS             complement(405..2480)
                     /locus_tag="PW224_001422"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2908116.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2482..3399)
                     /gene="glyQ"
                     /locus_tag="PW224_001423"
     CDS             complement(2482..3399)
                     /gene="glyQ"
                     /locus_tag="PW224_001423"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL2908117.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3783..4595)
                     /gene="recO"
                     /locus_tag="PW224_001424"
     CDS             complement(3783..4595)
                     /gene="recO"
                     /locus_tag="PW224_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL2908118.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4738..5637)
                     /gene="era"
                     /locus_tag="PW224_001425"
     CDS             complement(4738..5637)
                     /gene="era"
                     /locus_tag="PW224_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL2908119.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5652..6047)
                     /locus_tag="PW224_001426"
     CDS             complement(5652..6047)
                     /locus_tag="PW224_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL2908120.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(6031..6507)
                     /gene="ybeY"
                     /locus_tag="PW224_001427"
     CDS             complement(6031..6507)
                     /gene="ybeY"
                     /locus_tag="PW224_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL2908121.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6524..8716)
                     /locus_tag="PW224_001428"
     CDS             complement(6524..8716)
                     /locus_tag="PW224_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL2908122.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8740..9711)
                     /locus_tag="PW224_001429"
     CDS             complement(8740..9711)
                     /locus_tag="PW224_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL2908123.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10417..11796
                     /gene="brnQ"
                     /locus_tag="PW224_001430"
     CDS             10417..11796
                     /gene="brnQ"
                     /locus_tag="PW224_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2908124.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11952..12398)
                     /locus_tag="PW224_001431"
     CDS             complement(11952..12398)
                     /locus_tag="PW224_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL2908125.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12425..12601)
                     /locus_tag="PW224_001432"
     CDS             complement(12425..12601)
                     /locus_tag="PW224_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL2908126.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12763..13191)
                     /locus_tag="PW224_001433"
     CDS             complement(12763..13191)
                     /locus_tag="PW224_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2908127.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13283..14143
                     /locus_tag="PW224_001434"
     CDS             13283..14143
                     /locus_tag="PW224_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL2908128.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14227..14577)
                     /locus_tag="PW224_001435"
     CDS             complement(14227..14577)
                     /locus_tag="PW224_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2908129.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14593..16320)
                     /locus_tag="PW224_001436"
     CDS             complement(14593..16320)
                     /locus_tag="PW224_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2908130.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16533..17459)
                     /gene="trxB"
                     /locus_tag="PW224_001437"
     CDS             complement(16533..17459)
                     /gene="trxB"
                     /locus_tag="PW224_001437"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL2908131.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17604..19040)
                     /locus_tag="PW224_001438"
     CDS             complement(17604..19040)
                     /locus_tag="PW224_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2908132.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(19037..20440)
                     /locus_tag="PW224_001439"
     CDS             complement(19037..20440)
                     /locus_tag="PW224_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2908133.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20670..21572
                     /locus_tag="PW224_001440"
     CDS             20670..21572
                     /locus_tag="PW224_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2908134.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21613..23499)
                     /locus_tag="PW224_001441"
     CDS             complement(21613..23499)
                     /locus_tag="PW224_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2908135.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23596..24447)
                     /locus_tag="PW224_001442"
     CDS             complement(23596..24447)
                     /locus_tag="PW224_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2908136.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24905..26689)
                     /locus_tag="PW224_001443"
     CDS             complement(24905..26689)
                     /locus_tag="PW224_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2908137.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26689..28440)
                     /locus_tag="PW224_001444"
     CDS             complement(26689..28440)
                     /locus_tag="PW224_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2908138.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28582..28806)
                     /locus_tag="PW224_001445"
     CDS             complement(28582..28806)
                     /locus_tag="PW224_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL2908139.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            29057..30643
                     /locus_tag="PW224_001446"
     CDS             29057..30643
                     /locus_tag="PW224_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL2908140.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8675 a         6650 c         5522 g        10167 t
ORIGIN      
        1 ttttacactc tctgattgac gtcataaaaa gtagctttga attcataatc gtttatataa
       61 cgattaatag aaaacaatat agtatttagt gttcatacaa tgattctaag atcattttga
      121 attaatcagt cttatagttg atttctaaat tgtcatatat tattaaaaat ttttgccatt
      181 taaaaagaaa agcgacagtt taccaataaa tttcaactga attatctaaa agtagatcag
      241 ttagtttaga taattcagtt gaaattgacc gactcaaatt acttgtataa tacctaggaa
      301 aaaattaact agcaccacgg gtatagttat ttatatttat aacaaataaa ccagagaaat
      361 agcctatttt gtgcctattc tctggtttat ttacatatta gaaattattt tgtgatgatt
      421 tgatctaaac ttgctagcga caaagccatg gcagcaattt gacttaattg cgttaatcgg
      481 ttcttacgta ctgctggttc ttctgccatg accatcgtct gatcaaaata attttcgatc
      541 agtgggcgaa ggcttacaag ctgatcataa ttttcgttga tcgtttggtc agcaaagctt
      601 tttttcattg cctctactgc tttgtgcagt tctttctccg cttcattttc gaacagttca
      661 gggtctactt ctgattgttt ttcaagctcc gcttttttcg ctaagttcac gacacgtgtc
      721 aaagcttcca tcgatggttt gaagtcttca tctgcaagat gctgcttgaa caattgtgct
      781 gctgcaaata cttttgtcag atctttttgt tctgcattca aaacagcttc gatgacatca
      841 tgacgaatat tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcacc
      901 tgttgttgcc cttctgataa accaatgccg aaacgatccg tatctttact gataacatca
      961 ctaatcgttt cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgatccga
     1021 acgacaccgt atgcttggcg acgtaaggca taaggatcgt ttgacccgct tggaatcatc
     1081 ccaactgcaa agaaagaaaa aatgctatct aatttatctg caatcgctaa aacagaacct
     1141 actgtagact gaggtagctc tccttcactg gatataggca tgtaatgctc acgaatcgct
     1201 gttgctacag caggtttctc gcctttcagc agagcatatt tttcgcccat gatcccttgt
     1261 agttcaggaa attcccctac catgttggtt actagatcaa atttatagat ttcagcagct
     1321 cgttgcagat ccatcaattc ttcctcagat agtcctactt gttcgccaat gatacgggca
     1381 atgacttgta cccgctgcat tttctcatag atactaccga ttttttcatg gaaagtaaca
     1441 gtttttagtt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat
     1501 tccgcatctt ctaaacgagc cgtcaatact ttttgattgc ctcgaaccac attttccaat
     1561 tttacccgat caccattacg agcagaaata aaatgaggaa gcagcatacc ttgatcgttc
     1621 cgtacctcga aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca
     1681 gaaagatatt tttcttcgaa gtcgccagaa aagacagttg gatattcgac taaattgttg
     1741 acttcttcta gtaaatcttc gtctagatca ataacccagt gatttttttc ggctaattca
     1801 tttgcttgtg taacgatcat ttcttgtctt ttttgcggtg aagcaataac gaattgttca
     1861 ttcaattttt cttcatattc ttttgcatgc gcaaatgtca catcttcgcc taagaaacga
     1921 tgtcctcgag aggctctgcc tgttttgaca tctaagatct caaacgggat tacttcgtca
     1981 tctaacaatg ccacgatcca gtgaataggg cggatatatt caaaatcgta ttctgcccaa
     2041 tgcatcgtta ctgggaaagt gaggctttta atgacatctg ctaaacctgt caatacttct
     2101 ttcgcttctt tgccgtgagt gtatttcgtt acatagacat attccacgcc ttttaattct
     2161 ctaaaagtga tagcatctgt cgtcagacct tgtccgcgga caaatccttc agctgctttc
     2221 gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttca
     2281 ctgtctgctt gacgttctgg aatgtttgtt actttgattg ctaagcggcg cggtgtagaa
     2341 aacgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct
     2401 tctaattgtt tcatgcttgg tgtgacaaca tgagcaggca tttcttccag cccgatttca
     2461 agtaataagt cttttgccat cttattcagc ctccttcatg ttttctggat ttgctgtgtt
     2521 tttattcaat aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc
     2581 acgagccatg ttgcggatac gggaaagata acctgctcgt tctgttacgg atactgcacc
     2641 gcgagcatct aataaattga atgtatggct gcatttcaaa atataatcat acgctggatg
     2701 aacaagattt tcatcgatac agcgtttagc ttctttttca aaacggtcaa agttgcttag
     2761 tagcaagtct tggtcactta cttcaaaaga atatttggaa tgttcatatt ctggttgttt
     2821 gaagatctcg ccatatttca caccatcagc ccattccaga tcatagacac tttcaacttc
     2881 ttgaatatat gaagcaagac gttctagtcc ataagtgatc tcagaagtaa ctggtttaca
     2941 aggtagtcct ccgacttgct ggaagtacgt gaattgtgtg atctccatcc catcaagcca
     3001 tacttcccaa cccacaccgg cacaccccat cgatgggttc tcccagttgt cttcaacaaa
     3061 acgaatatcg tgttctaatg gatcaattcc taaaagacgc aagctttcta aataaagctc
     3121 ttggatattt tcaggtgaag gtttcattac gacttgaaat tgatgatgct gatacaaacg
     3181 gtttgggttt tctccataac gcccgtcagc tggtcggcgg gatggctcta cataagcagc
     3241 attccatggt tccggtccga ttgcccgtaa gaatgtatat gggctcatcg tacctgctcc
     3301 tttttctgtg tcataagctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa
     3361 tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt
     3421 ttaagcgttg ctgtcaataa gaatacagga aagaaaacga caaaaagtcc ctatgtctga
     3481 ttacagacat agggacgaat aatttcgcgg ttccacccta cttccgaata aagattcggc
     3541 agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggcttccact
     3601 ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt
     3661 tgcagttttc ttgtttatat aatagatcct ctcagtgaac gatctattta atctacggtt
     3721 aataatagac ggaagtccct gtatttgtca aatagttttg atttttttgt tgtatctttc
     3781 atttattctt ctttttcagg tatctctttt tcaggcatat ttttctctgg cgtctttaat
     3841 acgtgtgccc aagacttcat ttgatcaatg aactttttgc tttttagatg aataccgaca
     3901 tattcatcat acaactggtc aatgaggtca cggattgcct gcttcgtttc ttctttcaag
     3961 tggattccac tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa ataaacagcg
     4021 cgaggatctg catgataacg gtgttcgtcc attggccagt gtttctcgca tatcacaccg
     4081 ccagatttgg aagaatagtc aaattttcct tttgtttccc cgcatacgac acagtgcgtc
     4141 caaattggcg cgattccgaa gcgttgcagt atttgaactt caaagatatt cgtgatgatt
     4201 tctgcatcat ctccttgatc catccgctgc agagcttgtt gcgtaaattg gaataaatgc
     4261 gggtcatatt gatgatcttc gattgctgca tctactagat ttagcaaata ggtaccataa
     4321 ccgctcaaaa agatatcttc ttggatgttc cgaaacgcat ggacgtcttt gacactgctt
     4381 aaaaacgaca aaccttcact tcgaaaatta ccgatataga ctgcttcggt aaaggggagc
     4441 aaagccggag ctaatggctg attttgccga tggattcctt tgatataaaa catcattttg
     4501 ccagaagatt ccgtaaagat tttgactaat ttatcttttt ctttgtaatt acggctagat
     4561 aaaatcaagc ctttcgattc tgcaagctgg ctcatattct ccctcctaaa aaataaatct
     4621 ggaaaagcag attctgccga tccagattat ttatgtttcg atcttccgcc gtttttctca
     4681 acgtgcggat ttttatccag tttatttcta tacttaacct ataagaagtt cagaatatca
     4741 gtattcgtct tttcggtagc caaagtcttg cagatagact tttttgtctc gccagtcttt
     4801 ttggactttg acccaaagtt ctagaaatac tttgtctcct aaaagatttt cgatatcctt
     4861 tcttgctttc gttcctactt gcttcagcat ttttccgcct ttgccgataa tgattccttt
     4921 ttggctatct ctttcgacga tgatcgtggc ctggatatgg attttatcat tctcgtttcg
     4981 cttcatcgaa tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa
     5041 gactttctca cggatcaatt cagaaacgat aaagtattca ggatgatcag tcacttgatc
     5101 atctggaaaa tattgtgggc cctctggcat ttcactgact aatacatcca ttaagcgttc
     5161 gacattgttt ccttctgtag cagagatcgg aatcacttgt gcaaaaggca tctgcgtcga
     5221 ataatcttcg atgattccca gcaactcgtc tggatggatc ttatcgattt tattgataat
     5281 cagataaact ggagaatgca cgttttttag acgttcaata ataaaatcgt ctccccggcc
     5341 gcgtttttga tcagcactga tcataaataa gactgcatcc acttctttca acgcactgta
     5401 agctgcttct accataaaat ctcctagacg atgctttggt ttatggattc ctggtgtatc
     5461 gataaaaatc agctgagctt ctggtgtagt atatactcct tggattttat ttctagttgt
     5521 ctgtgccttg tcactcataa tggcaatttt ttgacccacg attcggttca ataatgtgga
     5581 ttttcctaca ttcgggcggc cgacgattgc gacaaaccct gatttatgtt ctggcatata
     5641 cgttctcctt tttaattaaa caacaaatga tatattttag ggacaaataa aatcaaccca
     5701 atgatgacag caaaacaagc ggtcactaaa actgctccag ctgccatatc tttgattttt
     5761 ttcccaatcg gatgaaaatg aaaatccgta aacatgtcca ctacattttc aaaaacagta
     5821 ttgatgatct ctactatcca aacgagaaaa acagctaaaa gcagccataa ccactctatc
     5881 cgatcaagct ggaaaatcac gcccataaat aaagcaagga taccgaacgc aacatgtttc
     5941 ctcatattgc gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa ttccacagac
     6001 atcaggaaat gtttgttttt ctctacgttt ttatcttttg agtccatagg catccaagat
     6061 ctctttctgt aaaccaaaca tttctttctc atcttctggt gtcatatggt catacccgtt
     6121 gatatgcaag aatccatgta ccgctaaaaa gccaagttcc cgatcatatg aatggccgta
     6181 agcttctgct tgttcagcag cacgttcaat agaaatcatg atgtctccta gtacacgagg
     6241 caaaggattc tctccatctt caaaaatcac tgggatttcg tcttcgcctt cttcttccag
     6301 agcaaaactg atgacgtctg tcggtgcatc ttttccacgg taatcacgat tgattacttg
     6361 gattgccgca ttgtccatga aagtaacaga catttctgtt tcctctggca gctttaaata
     6421 agatgctgca aattgcagca agtcatcgat ttctttcatt ttctcttctg agacttggtt
     6481 tgtttcatcc ataaaggtaa tatccattat acatcgcttc tttctattct tcttgttctt
     6541 cctttatctt ctcaacttct gttttcattt taggatattt gatccgagaa tggaaggtgc
     6601 tacttaaacc gctgatcaat gatttttcca cttttcggat ttctttcaag gtcagtccac
     6661 tatcgtctaa ttgaccatcg gaaatacgct cttcgataag attatggaca aaactttcga
     6721 ttttttcgct cgtcggatga tccattgccc gtactgcagc ctcacaactg tcagcaatat
     6781 tgacgatccc tgcttcacga ctttgcggtt ttggtccagg ataacggaat tgctcttctg
     6841 ttacttccgg attgcgttct ttcgctttga tgtagaaata acgcattagc gtggttccat
     6901 gatgctgttt gcaaatatcg atcaccattt gcggcatatt ataatcttcc aatattttgg
     6961 ctccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat aagaaattgt
     7021 gcgggttttc cgctcctgaa ggcaagttct caacgaaaaa gctagcatgt ttgatcttgc
     7081 cgatatcatg ataatagcaa gctactcgag ttagcaaaga acgtccgccg atttctgcta
     7141 cagcgttagc acttagatta gctaccatca tactatgatg ataagtacct ggcgcttctt
     7201 ccagcaactg tttcaataaa ggatgatttg gattgctgag ttcatttaat acaattacac
     7261 tgtcatcatt gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc aagaatgaga
     7321 agagtgcacc agtgaacccg cagacaagca ttaaccaagt cgttgcatca ccaaagctca
     7381 ttccttgata gacgatcaaa atgacatcca tcatcacagg aaaaataatg acccacatgg
     7441 cagcagataa tccttgttct gagatccgtt tgcgtctgac aatcgttgcc atgagcccag
     7501 agaaaacata agacatcaag ataatcgtta gaaagttcgt gccaatcgaa tcataaaaaa
     7561 tgaaaagtgc agatactgct tggaaaatac cagccatgat ccctgctctt cggttcaaga
     7621 aaaagcttaa aacaagtggt gcaaaagcag cgggataaaa cagcgggata taagtagctt
     7681 gttccgtttg gaataattgg aaaaatttca tcagcactat gctgactgac atagctgtca
     7741 catagaaaag cacgtatttt gttctttctc ctgcattttg gaattgaagt gaattataaa
     7801 tcagtacgcc gatttgaagc aaaacagcta aaaccatggc aatgagtggg aaaatcgaag
     7861 tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca tcaatttgat
     7921 ttccttcgcg aacaatgatt tctccttgga agatcatcac tggttgtaca gcttctcgtg
     7981 cagattgttt taattcttct gtctttttct cattcaatac atcattgact acaatgcctt
     8041 gatccactaa gtagcgaatc atttgttgct gctctgttgt gacattcaaa tactgaatct
     8101 gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt ttactcatct
     8161 gttcgtcaat gagcttcaaa ctttcatccc taaccgtgtg aatttccgtt tcagtcattt
     8221 caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg ttttctgcat
     8281 tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta ggtttcggga
     8341 tattttcatc ttccttcgcc ttatctacct tgtcattata agatttgttg atggtatcat
     8401 tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca tctgccagat
     8461 ctttttgata agtatattct ggagtcactg cttctgctgc cagttttctt ctttgttcgg
     8521 tttcttccgt gttctcgatc gttttattcg cacggatact ctcttctgct acttggcctt
     8581 ctctgaaatc attggccttt tgtttgacac tactaaatgt aacgccgcag acaatgataa
     8641 aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag tttttcttgt agtttctgca
     8701 gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat gaagatccgc
     8761 cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt cacttgcttc
     8821 aaaattaaca aaatcgattt ttttgatttg ctttagtgtt cgttctgcat gaacaagacc
     8881 acttgtcgtc cctcgaggca agtctatctg acttgtatcc ccattgacaa tcatttttga
     8941 ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt tttgtgcttc
     9001 gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta atggtgcgat
     9061 ttcgattacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa
     9121 agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc cgggtagaaa
     9181 gcctaggttt tcccctgcct ctacagcggg ccgcgtcaaa atgatttttt gcacttcgcc
     9241 tttttttagg gcagcaattg ctaatactac ggcaagaaac gttttccctg ttccggctgg
     9301 ccctacacca aatacgatgt catgtttggc taccgattca acgtatttct tttgaccgct
     9361 gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct catacatctc
     9421 aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacga catctggtgt
     9481 acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa
     9541 ggaaatctgg gattgttcac caatgatctg aatgatttct ccacgtgtat ggatggttgt
     9601 ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac
     9661 atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca aaaatagaca
     9721 tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa ctatatagca
     9781 aataagaaaa ggttgtaaaa gagtcgttta accatacgta ccaagaaacg ttaaaaaata
     9841 gctgtttcca ttcgctaaag gtctcgtttt gataaaaaga atctgagaat gtaagttata
     9901 tgaaagtgga gaacaaaata ttccgtttca ttcagaggta ttctctaggt atttttcttg
     9961 tttcaaaaaa agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa aacaggtgta
    10021 ttattacgtc aacaaataaa catacagcaa agcgtcgagg aaaacagtag gctattcaac
    10081 gatttattcc cagtgagtca tgtaagtgag aaatgacaat aaatcgaata gacgaacatc
    10141 atttccgagt caatgtcgaa aacgtagatc attgtggctg cctccattat caggctagcg
    10201 gataacttta tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt
    10261 accgttctag aatctagagc ccttttccca agtggaaaag ggcttttttt tatattttaa
    10321 ggtggtggtt ttcatggaat ctgatattag tggcattact aattgctgca acgtttatat
    10381 cgtcaatcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa aacttattca
    10441 tggaaacaac tattgatggt cagttcactt atcttcggga tgtttttcgg gtcaggtaat
    10501 ctaatctttc ctgtccactt aggtcaaatg gctggcgcaa actgggtcag tgctggcgta
    10561 ggatttgcca tctcaggcgc tcttttccca ttactagcta tcttagctgt agtagtgaca
    10621 aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc tgccttgttc
    10681 ttaatccttg ttcatttgac gatcggacca tttttcggta caccacggac agctgctact
    10741 gcttttgaaa tggctgctaa accatttttg cctgaaaaat ataattcatt aggtatgttt
    10801 ttattctctg ctcttttctt tttacttgct tatttcctta ctgtccatcc cacacggttg
    10861 gtcaaatacg tcggaaagta tctaaactcc ttgtttcttg cattattagc aatcattttt
    10921 gtcgtttctt tattccaccc aatgggaaat ctaaatcaat ctgctgcaga tacttaccaa
    10981 acaaacgctt taacaaatgg tgtcctagaa ggttataaca cggttgatgc agtagccttg
    11041 cttgccttaa gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa agataaaaaa
    11101 gtcacagaat taacagcaaa agctggtagc ctgagcgttg cgctagaagt catgatctat
    11161 tttggtcttg ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc agaaaatggc
    11221 ggaactgcac tttctcagat cactaatttt tatttaggta acttcggcgc tagcttctta
    11281 ggagtgatgg tcacattagg tgtcttcact accgctatgg gactcgttgt ttcttttgca
    11341 caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg gttaacgact
    11401 ttcgtctctt ttgtcgttgc aaatgctggt ttggacaata tcatccaatg gtctttacca
    11461 gtattgatgc ttttgtatcc attgtcgctt gcgttgattt tgctttcctt aacagcaaaa
    11521 tttttccaaa agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc tgttcccgcc
    11581 gtattggata tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt tgtagcaagc
    11641 atgctcgaat tttatcatca tcacgttcct tttgctgcat tgggacttgg ctggatggtc
    11701 ccaacgttac ttgggtatgc tggcagctta ctcttctatt acgcctatcg cctatcaggg
    11761 tataaacaag aagcaaatga actaccagaa gaataaccag atcaaacaca atttaacata
    11821 agaaatatta aacaggaaaa aaacagctag atgaaagaat cctattcttt cgtctagcta
    11881 ttttttagat ctctttttta cgagcacaaa aaaactgcgg acaatgattg tccgcagttt
    11941 aaatgataaa attaagattg taatagttct ttcacaatgg catttacttg gtttccatct
    12001 gcttttcctt tgactttagg catgatcgca cccatgactt tgccaaattc tttcattgat
    12061 gaagcaccag tttgcgtgat tgcttcttga acaagttgtc gaatttcttc gtcggaaagt
    12121 tgttccggca tgtatttttc gactatcaca atttcacttt ttactttttc agctaaatcg
    12181 tcacgtcctg cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac
    12241 aacaccgtta attcttcttc ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt
    12301 atagatgact tgatcattcg gatgacttgt aaagattcct tatctttcgc tttcatcgct
    12361 gttttcatgt catcgtttaa tctacttagt agtgacacac taccaactcc aattcatcac
    12421 agaactagaa ttttttgcgt tttctagctg cttcagattt tttcttgcgt cttacacttg
    12481 gtttttcgta gaattcacgt ttacgagatt cttgtaaagt acccgctttt gacacggaac
    12541 gtttgaagcg acgaagagca tcgtcaagtg attcgttttt gcgaacgact gtttttgaca
    12601 tgttaattcc ctccctccga gcttaaaaag taaccgtaat tgttattgaa atatatctca
    12661 aaaaacaaga ctgtcctgta aatattatat ctaatgctgt tttgaccgtc aaggaaatct
    12721 tgcttttttt taaaattatc aaatgttatt ttatttaaat ccttattgac aattttcaca
    12781 tcttccgaaa atctcgaatc tatgcccttc gatcgtacat ccaccgagct gttcttcgaa
    12841 ataattcatt ggacacatat ggatttcttt cgttgttccg cacaccgtac agataaagtg
    12901 atggtgatgg ccgatttccc cttgacagca atgaaaacga aacttcatct ctccatttaa
    12961 atcggtttct tccagtaaat cgatttctgc aaaatcatgg agatttcgat aaatcgtatc
    13021 gtaacttact cctttgaatt cctgattcat atagtcaaaa acttctttag cggatacgta
    13081 gcgattgcgc ttcactaaat aagttaacaa tgcttctctt tttttagtat atttaaaacc
    13141 gttatttttc aatgtttcta gtgattgttc gatcaacgtc tgttttttca tgacttattc
    13201 ctccaaatca aaatgattac gaagtacaca ttctagagta tggtataata aacataaaaa
    13261 aaatgcaagt gaggttttat ccatgtcttc cgattctaca gaaatcgtta ctttttttgt
    13321 catctccgac tctgctggag aaactgcgac aaaactagcg caagccacta tggcccaata
    13381 tcctagcgtt gagttcaatt tattccgcag aacatttgtg acagatgaga aaacattgat
    13441 gcaggcactg caagatgcac tagaagaaaa agctcttgtc ctgcacacat tgatcaatca
    13501 agaattgatc gagatgacgc gttctttttg tgaagaacat caattattta gttttgatgt
    13561 gatgactccg ccgatatcag aaatcgagcg cttaactggt atcaagccca ttcggcaacc
    13621 tggcgccctt catttattga atgagaacta tttcaaacgg atcaaagcaa tggaatttgc
    13681 tgtaaaatac gatgacggga aagatcctcg tgggtttcta gaagcagatg tcgttttgct
    13741 aggcgtatcc cgaacttcca aaacgccttt gagcttgttt ttagctaaca aaaatttaaa
    13801 agttgccaat ctacctttga tcccacaagc acatattccc aaacagcttt gggagatcga
    13861 tcctaaaaaa atcgtgggac tgacgaataa cccagatatt ttaaacaata tccgtaaaga
    13921 acggatgcgc tcttacggat taaatcctga tactgcttat tccgatatcg aaaaaatacg
    13981 agctgaactc gattttgcga atgatcttta tgaaaaattg ggctgcgtag taatcaatgt
    14041 cgcttcttta tctattgaag aaacagcttc gcttattttg aatgctttag acctagaaga
    14101 ccatagttat tatggaactg aaaccaccga agaaacagac taacaatttt tctttaaaaa
    14161 aactggactg tgtcagcgaa gaagattcgt tgacacagtc cagtttgtct ctatttgcac
    14221 ctatcgttat tgatgttcca catgtttgag tgtctggttc aaaatatcca gtacgtgaga
    14281 atcatcaaga ctatacaaaa tcgcttttcc ctcacgatgg gacttgacga catggttttc
    14341 tcttaataaa cgcagttgat gcgatacagc agattgttcc atttccactg cttggatgat
    14401 tgctgtcaca ttttgttccc cgtctttcag atacagcaga attttgagac gagtggggtc
    14461 actcaacatt ttgaatagtt ggctgacttt ctctactttt gttttttgcg tgtctggcaa
    14521 tccattcacc tccaacttga aaaaagactg acttctaaag tttaccactt tttccatcag
    14581 tctcaagttt ttttattctt ctgtcatctt ttggataatc gtttcataag cggctacttt
    14641 ttcagctgcg tcttctgaag agtttccttt tacaccgata taaaatttga ttttcggctc
    14701 agttcctgaa ggacggatag cgatccagcc attttcttcc agcgtatact tcaatacgtc
    14761 agatggcggt gtcgttagtt tttctacgtt tccttcacgg tctgttctcg tcaactcttt
    14821 gaagtcttct gtttggatga cttttgctcc ggcaaattct gttggtgctt cttcacggaa
    14881 tttttccatc aaagcagaga ttttcgcaga accttcttgt ccgctcattg tcacagaaat
    14941 cgttttttct gcgtagtagc cataggtttc aaaaatcgtt tgcaagccat catacaacgt
    15001 tttgccttgt tttttatagt aagctgctac ttctgctaat aagagcaatg cttggatcgc
    15061 gtctttgtcg cgaacgaaag gtttaactaa ataaccgtaa ctttcttcga atccgaacat
    15121 aaaagtatgg gaatgatctt cttcaaattg ttgaattttt tcagcaatga atttgaatcc
    15181 tgtcaataca ttgaacatgg tagccccata gtgttccgca attgctgtag gtaattcgct
    15241 agaaacgatc gatttcaata ccgctgcgtt ttcaggtaaa gtgcctcgcg attggtgtgc
    15301 ttccaaaata tattgagcaa acagtgctcc gatttggttt ccggtcaata cttcatagga
    15361 gccatttggc aaacgaacgg ctgcacctaa acggtctgca tcaggatctg ttgcaatcag
    15421 caaatcagcg ccttctttct cgccaagtcg tatcgcatac tcaaaagcag agtgttcttc
    15481 tggatttggt gatttgactg tagtgaaatc tgggtctgcg acagcttgtt ctggaactag
    15541 tacgaattgt tcgaatccag cttgtttcaa cgcacgttcg cccaacattt ttcctgttcc
    15601 gtgaagtggt gtgtacacaa gtttcaaatc tttccccatt gtttccacca attcatgatc
    15661 aatcgttact gctttgattt cttctagata agcggcatcc acttcttctc cgatgatcgt
    15721 gatcaatccg ctatgttcag cttcttcgtc agataatact tcaactttta acggattgct
    15781 gacttcacgg acatatttcg tcaatgcatc tgcgtctgca ggaggcattt gtcccccatc
    15841 ttctccgtag actttatatc cgttgtaagc tgctggattg tgggatgcgg ttaccatgat
    15901 tcccgcaaac gtcttcaaat gacggactgc aaaagaaagc tctggtgttg gacgtagact
    15961 ttcgaataca taagatggga tattgtgatg tgctaatgtt ttggctgctt ccatggcaaa
    16021 ttctggtgat tgatggcgtg agtcataagc gatagcgaca cctcggcgtt ttgtttcttc
    16081 gccttgtgta tccatgaaac gtgcaagtcc ttctgttgct tgtcttaccg tatagatgtt
    16141 cattcgattg atcccaggcc ctaagattcc ccgcatgcct gcagtaccga attccaacgg
    16201 tgcataaaaa gcatcttcaa gtttttcagg atcttcaccc agatcattta attgtttttt
    16261 taagttttct tctaaatttt cttcatgtac ccattgttca tacacttgtt cccaagacat
    16321 gaaaataaca cctctttctg aaatatggtt cttaataagt atagcattca cttattagat
    16381 atgaaagtat gaggaccaaa aaacgcttaa atcaagaaag gattctttta tcaagcacgg
    16441 ttgcaagtat tcgcataaat cggattcagt agatttcacg cgaaaaaaaa gagtaaaaga
    16501 acaattaaac cgttcttcca ctctttttaa acttatgcag ttgtttcttc taaagattcc
    16561 aagtatttat acacttgttg tccagcgatc ccaccatcac caacagcagt agtgatttgt
    16621 cgcaaatctt tttcacgaac atctccgatg gcaaaaacgc ctggtatatt cgtcttcatt
    16681 tcgttgtctg ttggaatcca gccagcttcg ttcgtcaacc ctgcttctct aaatggtgca
    16741 gttaatggat ccagtccaac atagatgaat actccgtctg ctgccaaatc aaactcattg
    16801 ccggttttga cattacgtgc tttcacacct gtcactacca tttcattccc aacgatttcg
    16861 tcaacgactg tatcccacat aaaggaaatt ttttcattcg caaatgcacg gtcttggatg
    16921 atcttttgtg cacgtaattc atctctacgg tggacgatga ctacttcaga agcaaattgt
    16981 gtcaaataga tggcttcttc tactgctgag tctcctcctc cgatcactac tagacgtttg
    17041 ttacggaaaa acgcaccatc acaaactgca cagtaagaaa cgccacgtcc agcaaattct
    17101 tcctcgccag gtacgcctaa tttacggtgg atacagcctg ttgcgataat gactgttttt
    17161 ccttcgtaag ttttatcttc acaaataact tctttgtggc tgccgtgatc cttgatatct
    17221 tgcacgattc cataagcatt ttctgtccca aatcttgaaa caccttcata catcttataa
    17281 gctagatcag gacccattat tgaatcgaaa ccaggatagt tttcgatttc tgccgtattg
    17341 ttcatttggc cgccaggggc gccttgttcg atcatcaata cggataagtt tgaacgtgaa
    17401 gcatataaag cagcagtcat cccagcagga cctgcgccaa taacgattac atcatacata
    17461 tgtagattct cctttactta atcaagtaag catactttac agccaatgcg aaaaaaagtc
    17521 tattgttctg cttgcgtttc tcgaatagct ttattatact cgaaaaatcc gctgaaaaac
    17581 aatagacttt tataaatcat tgatcaatca ttcgaaagat tttctccatt tgaatcaatt
    17641 acttttttat accagttgaa tgatttcttc ttcgaacggg ctaatgtgcc atttccttcg
    17701 ttgtcacgat cgacataaat gaatccgtac cttttcttca tctcacctgt accagctgat
    17761 actaaatcaa tacatcccca agtcgtatag cctaatagat cgactccatc taattctact
    17821 gcatctttca ttgcctggat atgtgctgcc agatagtcga ttcgatagtc atcttccaca
    17881 taaccattct catcgggcgt atcgactgca cctaagccgt tttctacaat aaatagtggt
    17941 ttttgataac gatcataaat atcattcatc gttattctca agcctaatgg atcaatttgc
    18001 caaccccatt cacttgcttc cagatatgga tttttgacag aagcaaagat atttcctgca
    18061 gtctgttcca agagttcgtt gtcagtagtc gaaactcgag aagaataata agaaaaggag
    18121 ataaaatcaa cagtatgttc tttcagcaat gcggcatccc caggttccat tttgatctga
    18181 atgccatttc gttccatttc tttcaatgca taagcaggat attctcctcg tgactggaca
    18241 tcaatgaaga aataattttc tcgatctgat tttctagaag cccaaacatc ttcaggattg
    18301 caagtgtatg gataatatgc tcctgctgcc agcatacagc ctactttgtt ctccggatct
    18361 acttcatgag caatcttcgt tgcgatggca cttgcaacca actcatgatg tgccgcttga
    18421 tatttgattt gttcttcatt ttctccttct tcaaaataca gccctgcgcc catgaatggt
    18481 gcatgaagga tcatattgat ctcattgaat gtcaaccaat atttgacaag tcctttatag
    18541 cgattgaaga tgactcggca cagattttca taaaaaccaa cgagttctct ggaacgccac
    18601 gcaccatatt ctttgatcaa atgcatcgga caatcaaaat gtgtaatcgt cacaagcggc
    18661 tcgatcccgt acttttgaca ttctttaaag acatcctcat aaaatttcaa accagattca
    18721 tttggctctt tttcatcgcc cattggaaaa atacgactcc aagcaatgga catccgataa
    18781 gtcttgaacc ccatttctgc aaataaagca atatcttcct tataatgatg atacatatcg
    18841 atcgcaattt ttgctggata aaaatgattt tcatcaaatt caaacatttt cttttttcca
    18901 gtaatgacag gaaaacggtc ttctccgatt ggcacaacat ctacgttcgc taacccacgt
    18961 ccgccttcat cataaccgcc ctcgcattga ttggctgctg tagctccgcc ccataaaaaa
    19021 tcttttctga atcccatcat acttcctcca ttacatgtgt attgcttgat aattctcttg
    19081 ccagttcatc ttggacttcg attccttctg cttttaaccg gctgattgtt tccttgaatt
    19141 gcggtaaata ctgctgatgt gcaataaaca actcattcat gatccgtttt gctgtctttc
    19201 ctgatcgtat aagcggattg atcgtaaacg cttgtaaagc tgttccataa tcaccagtca
    19261 cagctgcttc gatcgtcagc aattccatat tcttcattac ttgcaaccag cctctttcag
    19321 cagcaggaag ggaaccaaac gctacatttc gtgcgccttg tgcgccaaca taggcagtga
    19381 cttctactac acaatctgcc ggcagatcag gaacagctcc atcattcttt gtagatacca
    19441 cgatctgtgt ttctttattt ccatagatcg aagcaatcgt ttcacaggca gcatctgaat
    19501 aataagcacc acctcgtttt tgcaattgtt caggtttatg atccaattct ggatctttgt
    19561 ataattcaaa cagctctgct tctgtttctt tcacttgctg cgctcttgta ccaatcgttt
    19621 gatattcttc taaagcatgt tccagcattt cttcttcacg gtagtaatag cggtggtagc
    19681 cacaagggat catgttcatt tgttttaatt gttctttgaa aaacgggata tcaaagatat
    19741 ttttcggcaa tccattgtct tcatcgtata aacgatcaat caattctgcc gtgcggtcat
    19801 ttcctttatt atctgctact ttgtgccaat ggaagtgatt tagaccggca aacttgtaga
    19861 tcagctcttc ttttttcttg ccaagcattg ttggttctgt catcatcgcc attactggca
    19921 cattgcataa tcctatcact ttatcccatt tgccatatct gataactgct tctgtcacca
    19981 tcccgcttgg gttcgcgaaa ttgatcagcc aagcatttgg acataaacgc ttcatgtctt
    20041 caacgattgc caaaaccact ggaacggtac gaaaagcttt gaatatcccg ccagcaccat
    20101 ttgtttcttg cccaatcatc ccataataac tagggatccg ctcgtctttg attcgtgcat
    20161 ctaattgacc gacacgaaac tgagtcgtaa caaaatccgc atctttcaat gcttcttccc
    20221 gatccagagt tgcatagatt ttgacttcat acggagaagc atcccacata cgctgagaca
    20281 tctgactgac gatctcaagc ttttcttttc cttcttctac atctacgagc cagatttctc
    20341 gtatcggtaa ttcctcatat cttttgataa atccttccat caactcgggg gtgtaactgc
    20401 tgcctccacc aatcgtagcg attttgattc cttttgccat gttctcactc ctttactttt
    20461 tacactttta ttatcttttt tgtaaacaaa acaaacaata gaaattcgtt tctttccagt
    20521 aacatttaca aaagttagtg ttttctactg gatttttgta aatatcttgt caaaaaatgt
    20581 ctttattaag ctatataaca taaatccgtt tacaaatcat tttcaaaaaa gttataattt
    20641 acgtaaaaaa acaagaagaa ggtatctaca tgttattacg tgaaaaaatc aaagaaatct
    20701 ccttttctcc ttcagaaaat gaagtgattc agtttctctt aagcgattct ccagcacttg
    20761 gcgaaatgac catccaagag attgctgagc gatgttatgt ccacccgtcc acactcatcc
    20821 gtgtagcaaa aaaaatgggt tttgatggct gggtcagttt tcgtaatgct tttttagctg
    20881 aaacagccta tcttgaaggg aattttcaag acgtagatgc gaatcttcct ttttccacca
    20941 cagacggtat tatgacgatc gcaaataaaa tggcttcttt agaacagatg acgattcaag
    21001 atacactttc tctcattcat catgaccaac tgcaaaaagc caaacaacta atgcttcaag
    21061 caaaaaaaat cgtgattttc gccagtaact cgaatacttt gatttctcaa gatttcatgc
    21121 tgaaaatgaa gcgcatcaaa atggatgtca cgattgtcac cacttttggt gaatcgttct
    21181 atgaagcgta caattgcacg agtgatatgt gcgggattct gatttcttat acgggtgaaa
    21241 acaatgtgat ccttcgtacc gcagaaatat tgaaacaaaa tcatgttcca attttagctt
    21301 tgaccagtat cggtgattca acgctatctt caatgagtac agccgctttg cgacttacga
    21361 cacgcgaacg actgtattcg aaaattggta attttacgat caatacatcg atttgttatc
    21421 ttctagacgt gttatatagt tgtgtttttg cagaaaatta tcaacagaat ttagtccatc
    21481 taatttctgt aggacaaaaa tttgataaac gccctatcag ctcagccatc atgaaagagc
    21541 ctttcaattt tgaaaataaa gaagtggact aaaaagaata attaagccta gtccattctt
    21601 tcacctaacc gttcaggtca gtgcggtgat aagtgcatcg tctggcccga ccgtctcccc
    21661 ttgattttcc aaaatatcca aataatccgt gtaattggtg acgatcacag gtgtttctaa
    21721 actgtatcct tcctcttcga ttttcttttt gtcgaagcgg agcaacagat cgccttgttt
    21781 cactctttct ccttgctgca ccaagctttc aaaatatttc ccttccaact gaacagtatc
    21841 caatccgaca tgaatcagca attccagacc tgcttcagaa accattccga ttgcatgttt
    21901 cgtcgggaac agggtcatga ctgttccgtc aaaaggggca cgtacttctc ctttttcagg
    21961 gtagatcaag attcctcgac ctagtgttcc ttgtgcaaaa gctgcatcct ttgctgcatt
    22021 cagtggagcg atttgtcctt gtatcggtgt ttttacaatt tctttacctg atcttttctg
    22081 tctatcatct gtttcacctg cttcctcctt ccaaaagaaa aaagtcagcg caaaggatac
    22141 caccatcgca atggcgattc caatcaatac attaatcaca tccgttaaat caccatctgg
    22201 tctaatatag ttgggaattg taaagatgcc taatccacct ggattccaag aagttaaatt
    22261 cattaatcca ctatacgcgc cccctaacgc accaccgaca caggtaaaaa taaatatttt
    22321 tttacgtggc aatgtaaacc cataaatagc tggctctgta atccccacaa taccagaaac
    22381 aatggctggt attgcgattg ctttgtcttt tgggtttcta agcttaaagt agagtgcagt
    22441 aagtgctgca gtttgtccaa aagacactac tgcaacaggt gtcatgatat tttgccaacc
    22501 attcacagaa aattgcatca atcccattgg tacgattgcc caatggagac caaacatcac
    22561 tagaatttgc cagaagaagc ctacgattgc tccataaata actggcgaaa gtgctaaaag
    22621 tgtttcaaat ccagccatta aaatatcagt tgcgaatgtg aatgctggtc caacgagtaa
    22681 aaaacctaag ggcattgtaa taagaacggt gaagaatgga acgaagaaat tcgcgatcat
    22741 ctcaggtact aactttttaa ttggtttctg aatttggcta gctaacaaca caataaagat
    22801 aatgggaatg acagaagaag cgtagttacg cgcaatccag ggaatgccga atactgtttg
    22861 ataaataggt gcttcaaaaa tagtgccaga aaatatggta gtcagtggtg cttctgctgc
    22921 accactcaaa gcttccagcg acaagctagg attcattaat gctgccccaa gcaacatccc
    22981 tacgaattca tttactttaa atttacgggc agctgttaaa ccgataataa caggcataaa
    23041 ttggaataca gaatcaccga tattatcaaa cataacaaat gtgccagaac cgcggtcaaa
    23101 tgcgcctaag aagacaagta atgctgtcag tccacgaatc atcccaccag cagctagaat
    23161 agcaaggaat ggctggaaac agcccgatat tccatcaata agcatatcaa acggtctcat
    23221 tttttctgtt gaaacagcct catcaaaagt aaggtttcct gcttttataa cctcttcaaa
    23281 cacgactggc acatgattcc cgatgacaac ttggtactgt cctcccgctc gcattaccgt
    23341 cacaacaccg tccatatttt tcaagatctc atcatttgct ttttcttcgt tttttaattt
    23401 gaaacgaagc cttgtgatac aatgtgtcaa accattgata ttttccttcc cgccgacatt
    23461 ttctacaatt ttgctcgcta attcttgata ttttcccatt tctattccct ccaatttatt
    23521 agtggaaggt ttagccaact gaatgtcact acccttattt catgcgttct tgttaattta
    23581 tcctcctttt tttatttatt tacaacgatt ctcatgcact aaacgcgcaa tatggatcgt
    23641 taaatacatt ttttcgtccg tggacatctg gtagttgaat atctcggcta aaaagtctgc
    23701 tattttctca acgcaaccaa acgtatcctt atattttttt tgaataacct ggtacaattc
    23761 ctcagaaact tctcccactt ctgatttttc tccatctagg acgcgtgtca gaaaaaaacg
    23821 taggtgtgtg acgaatcgaa aaaaatggac tgaatcttca tcaaatgtaa tcttatagta
    23881 atagcggacg atatttagaa tatcctgtat tgttgtagta aacaggtagg tattgtcttg
    23941 ttggctttga atgccttgtg cattgactaa atgcaatgcg ataaatccgg cttcatcttc
    24001 tgataacttg atgtggtatt tatccgagat tttcttcact gtttttttcc cgatggcata
    24061 ctctttagga aatagttgtt tgatatcata cagcaataca tttggtacaa atattcccat
    24121 tttgtgacgt tcgatagccg tatacaaatg atccgtcaat gcaatataca ccgaatcatt
    24181 caactggact tgtagagcct cttttgtata ttcaatgatt tcacaagaaa tttctaagta
    24241 ctcaagagga atcgcttgtg ccaattcttg gaactgttgt gaaagtccgt catttgtcaa
    24301 ctgaaatttt ttgtcgatgc atgagtcaaa aatcgggtca cccactttct ttttgaatgc
    24361 caaacctttt cccatcacga tcatttcctg atttctttca tccctggtta ttacgacgtt
    24421 gttattcagt actttctcaa tcagcatgtt ttactccttt ccatgatata tagggctgta
    24481 tccaaaacaa aaagaaaaac ccaaaaagcc gtccatggaa ccatgggaga ctcatcaggt
    24541 ttagcttgca tcaggcaatc actatccaag ttgtatcgtt ttctttatat tatcgaaagc
    24601 aagcactttt gtcaacgctt tcttagcggc tattgggcac ttctgactag ctgtcgtgat
    24661 tttttttaaa tctggtaatt acgacactaa cgctcacaga tgaagaatct gggaagctct
    24721 gttttatttt cactctagcg cttaggtcaa aatgctacta tgctactgtc ccaacttcta
    24781 atatacggtg ttccagtacc agcttatcgg tgataagcca aatttatgca aaaatctgga
    24841 aagccttttt tacaattttt ttcttatcac ctaaagctga acggagccct gtcacaacca
    24901 ctttttacac accactgctt tgcagtttgt acatatctgc atacaaacca ttttgcgcta
    24961 gaagtgtttc atgattccca cgttctacta tatgaccttt gtctaaaact aaaatcaatt
    25021 ctgcatcccg aatcgtggac agccgatggg caatcgcaat cgttgtccgt ccttgtcgca
    25081 tttttgtcaa tccttcttgg atcaatcctt ctgtttcagt atcgatattt gctgtcgctt
    25141 catccaatac tagtatttta ggatctgtca cgattgttcg cgcaaaagtg atcagctgtc
    25201 gttgtccgct agaatagctg gcaccgtgtt cgatcacttt tgagtgatat ccttttggta
    25261 attcatcgat gaacttgttt gcttgaacaa actcagctgc atggcgaatc tgttcgtctg
    25321 tgatagttgg attcatcatc cggatatttc ctgcgatatc tccataaaac aagaaggcat
    25381 cttgcaagac caatcccatt ttcttacgta actcttccat tggatattct cgaatgtcat
    25441 gatcatcaat caagatctgg ccttcataaa attcgtaaaa ccgcatcaat acgttgatga
    25501 tcgagctttt tccgcttccg gtgtgcccca ctaatgcaac ggtctctccc ggattagcga
    25561 caaaagagat attgttcaat acattgtgct ctccatcata agaaaagctt acattgcgaa
    25621 actcgatctt acctgaagta atcgcttgat ctgctccagg attttgttgc ggtgcatatt
    25681 cttctgtatc catgattttc aaaatacgac taccggcaac cattccatct gtaaaggtgc
    25741 tcaaaaagtc catcatttga gacattggat taaaaaatcc ctggacatag gtcgtaaatg
    25801 catagatcag tccaacttct acgggagaat gcagcgcatc gatcccaaac aaagtcaacg
    25861 ccaacgcaat cgccaatgca tatagaaaat tgatgatcgg acttaacaga atcgaatttg
    25921 ttttgatcat cgcatagcgc gtgtctaaat actcgttgtt cacttgttca aattctttag
    25981 ctaaacgagc ttcttggcga aactgctgga taatacgcat gccggaaatc gattcattca
    26041 atttcgtgtt cagctgactt aatttttccc gcatattccg gtaaatacga gaactgaatt
    26101 tctggtaata ccagatgacg attcctaaaa tcggcaagaa caccaaagtc cacaaggcaa
    26161 tttctccatt gatttgaaac atggcaatga aggaagaaac gactgcaaag atcccagtca
    26221 ataccatcaa aaagacatac caaaattcaa atagcgtttc tgtgtcattt gtcactcgag
    26281 aaacagtcga tcctgccggt gtctgatcaa aataacgcat acctagtgta tgcagctttt
    26341 caaataatct tacccgaaca tactgatagg tcttcaatga agccatggaa tacaaatacc
    26401 attggaaaaa ccaaatgata cttttgacta tgactccaaa caagtacata ccagcaaaaa
    26461 acaaaataac ttgtgtcgta gccgactgtt tagccagatg atcatccata aaagtttgga
    26521 tgatccttgg caataggaca ttgatcacag acagggcaaa cgcaaataag atagcaacga
    26581 tgaacgttct tctaaaaggt ttggcgaact tcatcagtcg tttgatgatc ttcttctgtt
    26641 ctttaagtgg aatcgatttt gtccattccg attgataatt ttcatccatc aggcctcgcc
    26701 tccttctatc ttcgcttcta atttctgttt ctcccacatt cgttcatacc agccgcctaa
    26761 tgcaagcagc tcatgatggg ttcctcgttc aatgattttc ccttgatcga caacaatgat
    26821 ttcttttgca tgcatcacgc tgcttaaacg gtgagctgca atgatggttg ttttctcttt
    26881 tcttatttct tttaaattat gcaagatagc ttcttctgtt ttggcatcta ctgcagaaag
    26941 tgcatcatct aagatcagca attcaggatt tttgatcaat gctcgagcaa tcgaaatcct
    27001 ttgtttctgt ccgccagata aagagactcc acgttctcca actaaggtat cataaccttt
    27061 tggtaattcc ttgatttcat tgttgataaa tgctaaagaa gccgcatgct ctacttcttc
    27121 ttctgaaagt tttggattag aaaaacggat attatctcga atggtcatcg aaaacaagaa
    27181 gtgatcttga ggcacatagc cgattgatcc taataaagca ttcaatgaat attctttgat
    27241 cgaacatccg ccaaattgaa tttttccttg ataatcatcg tattcacgca tcagtaattt
    27301 gatgattgtc gtttttcccg caccagtttt cccaacaata cctaatgttt ctccttcttg
    27361 gatcgcaaat ttgatttgtt ctaatgctgg tgattcttct ttcggatagt taaaagagga
    27421 aatagctaca ttcagctcgc cttttgccgg agtctgcact gcacccagtt gttccacgat
    27481 atgcgtcttt tcatgcaaaa gttcattgac gcgatcataa gaagcatttc ctctttccaa
    27541 tacgttgaac aatcgaccaa tcgcaaacat cggccaaaca agcataccga tatagctaat
    27601 aaatgagacc atctgcccga ttgagatcgt gccttccaag acatactttc cacctaaaat
    27661 aattgttgca acataagaca gaccgataat caaggtaatg aatggatcaa ataaggagtc
    27721 taaaaaattg actcgtttgt ttttaataat tgcatcttca atcttttctt caaaatccgc
    27781 gagatcttct ttttcttgtc caaatgtttt aatgactttg atcccagtaa tactttcttg
    27841 tgtcttatca ttgatactag aaaaagcttc ttgcgaatca cgaaaagcat cgtggagttt
    27901 tgaccccagc actcgtgaag taacggctag taaaggcaga ggaatcagcg caatcagtgt
    27961 taagcgccaa tcaataaaca aaatcatcgc aatgatcgtt gttccgcccg tgatcactga
    28021 atctgcaaag gtcaagatac cagctcctgc tacattttgg attgcattca aatcattcgt
    28081 tgcatgagcc atcaaatctc cagtccgata tttttggtaa aagagattgt ccatttctgt
    28141 aaaatgtttg aatagctgct gccgaagttc tttctccaac cgagctgcac ttccccaaat
    28201 attcgtccgc cagatataac gaaaaagata ttgtaagaga gctgcaagga tcaataataa
    28261 gatccaccag gaaatgatct ttattgataa atttttatcg gcgatctcat caatgatgat
    28321 cccaatcact tttggaggag ctaactgtac caaggccact gcgaataggg agaaaatccc
    28381 aattaaataa tgtttctttt cctgtcgaaa aaaccaacct aattttctaa aaatcgacat
    28441 tttactcctc ctttgtcttt atcaagagca taggattgtc tgtgcttgtc ttttttgctg
    28501 aatagtctga atagtctaac aaattacgaa ataaaaaacg gactgaaaaa atcagcccgc
    28561 aaaaagtctt tgggtcagaa cctatttttt cccttggttc ttcatttgct gcatcatctg
    28621 gcgaattttc ttctcagatg gtttttgccc cattgacatc atcattgatc gcaacatgtc
    28681 ctcattaacg ggaggatttt tttctaaata gtctttcata tatttacgcg ctaggaaaaa
    28741 gccacctact gcgccgatta gcaaagcaat aatagcaata agtacgacaa tacctgttga
    28801 taccatataa atctctcctt tctatttccg aattgctctc aatgatttat tttactaaag
    28861 tttattgaaa aaagaaaggc gtattcttta gtttcatgca aaaaatagtg gtttttttag
    28921 agaagaacaa aatttctttc attaaccagc aagacacgcc acgaaaaaaa attaggcata
    28981 ccaaatcttt ttctttaatt ttttctttct gaggtgtaga atgtaatcgt agataaatag
    29041 gaaaggtggt tttttcttga ataatcaatc caacaaacaa tttcataagc atcgacttat
    29101 tgaacataca aatggtttat ctttagctga agtaaatgga acagtggatg tacccaaggg
    29161 aaaaagtttt tttaaaacac ttcttgctta ctctggacca ggtgcattag tagcagttgg
    29221 ttatatggat ccaggtaact ggtccacttc gatcactggt ggacaaaatt tccaatattt
    29281 attgatgtca gttattttga tgtctagtct gattgctatg cttttacaat atatggcagc
    29341 aaaattagga atcgtgaccc aaatggattt ggcccaagca atccgagcac ggacaagtaa
    29401 gacattgggt gtcattttat ggatcttgac tgaattagcc attatggcaa cagacatcgc
    29461 agaagtcatt ggtgcagcaa tcgccctcta cctgcttttt gatattccat tggtaattgc
    29521 cgtatttatc actgtgttag atgttttcgt ccttttattg ctgacaaaag tcgggtttcg
    29581 taaaatcgaa gctttggttg tctgcttgat tttcgtcatt ttattcgttt tcgtctatca
    29641 agttggcttg tctaaccctg attggcacag tttgtttgcc ggatttatcc ctaacagtca
    29701 cacattctca gactcaccgg tcatcggcgg cgaaactccg ctgactggag ctttagggat
    29761 tattggtgca acagtcatgc cgcataactt atatcttcat tcagcagttt ctcaaactcg
    29821 gaagatcgat caccaagacg aagaagatgt agcacaagcg gtacgctttt ctacttggga
    29881 ttctaatatc cagttgtcaa tggcttttct agtcaatgca ctgctattga tcatgggtgt
    29941 ggctgtattc aaaaccggtt ctgtccaaga tccttccttt tttggtctgt atgaagcttt
    30001 atctgatcca tcagctatga gcaatggtat cttgattgct gtagcaaaat ctggtatctt
    30061 gtctacttta tttgctgtag cacttcttgc ttctggccag aactctacta ttactggtac
    30121 gttgaccggt caagtcatca tggaaggctt catccatatg cgtatcccaa tttggctaag
    30181 aagactagtc acacgattgc tttctgtgat tcctgtattg atttgtgttt tgtttacaag
    30241 tacaaaagga acgatcgaag aacataccgc tttgaataac ttgatgaatg aatcccaagt
    30301 ttttctggct tttgcattgc cattctcgat gattccttta ctgatgatga ccaatagcca
    30361 agcggagatg gggagccgat tcaaaaattc atttgttgta aaactacttg gctggttctc
    30421 tgtgattagt ctgacttact tgaatttacg aggattacca ggtcaaatcg aaagtttctt
    30481 cggcgagcaa gccacctcag ctcaactagc attggctgat caaatcgctt atgtaatcat
    30541 tgttcttgtc cttctcctgt tggcatggac gattggtgat ttatataaag gaaacaaaaa
    30601 atatgaacag cgtttgcagg aaacgataga agaaatcgag tagaaactga ttcttttaca
    30661 caaaggaggt tgtgtcagaa gtgggcagct tcaagaaata agaaggaatt taagaaaatt
    30721 gcttttcaaa tttttgtgaa atttcggctt atttccgaag aagttacttc tggaacaccg
    30781 tgtatcagag gttgtgaaaa agctgccttg catcaagtaa taagaacagc caagtaaaaa
    30841 cagcttttta tgtttttaac ttggttggac ttattaccgc agatgcaagc ttttgagcac
    30901 cgtttattcg gaaaaaggaa ctgcgacaag acttttgtca cagttccctt tcctacgatt
    30961 agtcaagtaa aaggtgtaag aaaaatagct cataaacgaa agcgacaaaa tcaa
//
LOCUS       DANKMN010000035        29099 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000035 DANKMN010000000
VERSION     DANKMN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..1031)
                     /locus_tag="PW224_001447"
     CDS             complement(<1..1031)
                     /locus_tag="PW224_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2908141.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="PW224_001448"
     CDS             complement(1113..1802)
                     /locus_tag="PW224_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2908142.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="PW224_001449"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="PW224_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL2908143.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="PW224_001450"
     CDS             complement(2329..2832)
                     /locus_tag="PW224_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL2908144.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="PW224_001451"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="PW224_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL2908145.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="PW224_001452"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="PW224_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL2908146.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="PW224_001453"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="PW224_001453"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL2908147.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="PW224_001454"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="PW224_001454"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL2908148.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="PW224_001455"
     CDS             complement(6444..7463)
                     /locus_tag="PW224_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2908149.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="PW224_001456"
     CDS             complement(7636..8541)
                     /locus_tag="PW224_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2908150.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="PW224_001457"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="PW224_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL2908151.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="PW224_001458"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="PW224_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL2908152.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="PW224_001459"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="PW224_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2908153.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="PW224_001460"
     CDS             9754..10398
                     /locus_tag="PW224_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2908154.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="PW224_001461"
     CDS             complement(10454..10957)
                     /locus_tag="PW224_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2908155.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="PW224_001462"
     CDS             11554..12519
                     /locus_tag="PW224_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2908156.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="PW224_001463"
     CDS             complement(12618..13790)
                     /locus_tag="PW224_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908157.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="PW224_001464"
     CDS             complement(13777..14922)
                     /locus_tag="PW224_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908158.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="PW224_001465"
     CDS             complement(14936..15868)
                     /locus_tag="PW224_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2908159.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="PW224_001466"
     CDS             15985..17064
                     /locus_tag="PW224_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2908160.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="PW224_001467"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="PW224_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17789..18016)
                     /locus_tag="PW224_001468"
     CDS             complement(17789..18016)
                     /locus_tag="PW224_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2908161.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="PW224_001469"
     CDS             complement(18119..20707)
                     /locus_tag="PW224_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL2908162.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="PW224_001470"
     CDS             complement(20727..21386)
                     /locus_tag="PW224_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2908163.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="PW224_001471"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="PW224_001471"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL2908164.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="PW224_001472"
     CDS             complement(22159..23025)
                     /locus_tag="PW224_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2908165.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="PW224_001473"
     CDS             23134..23775
                     /locus_tag="PW224_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL2908166.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="PW224_001474"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="PW224_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL2908167.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="PW224_001475"
     CDS             complement(25216..26115)
                     /locus_tag="PW224_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2908168.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="PW224_001476"
     CDS             complement(26117..26914)
                     /locus_tag="PW224_001476"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL2908169.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="PW224_001477"
     CDS             complement(26892..27596)
                     /locus_tag="PW224_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL2908170.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="PW224_001478"
     CDS             27727..28302
                     /locus_tag="PW224_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL2908171.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="PW224_001479"
     CDS             28420..29097
                     /locus_tag="PW224_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2908172.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8563 a         6167 c         5231 g         9138 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaa
//
LOCUS       DANKMN010000036        28213 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000036 DANKMN010000000
VERSION     DANKMN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            114..1949
                     /gene="tdc"
                     /locus_tag="PW224_001480"
     CDS             114..1949
                     /gene="tdc"
                     /locus_tag="PW224_001480"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2908173.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2254..3732
                     /locus_tag="PW224_001481"
     CDS             2254..3732
                     /locus_tag="PW224_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2908174.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3698..5030
                     /locus_tag="PW224_001482"
                     /pseudo
     CDS             3698..5030
                     /locus_tag="PW224_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5144..7696
                     /locus_tag="PW224_001483"
     CDS             5144..7696
                     /locus_tag="PW224_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2908175.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7703..9142)
                     /locus_tag="PW224_001484"
     CDS             complement(7703..9142)
                     /locus_tag="PW224_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908176.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9488..9850
                     /locus_tag="PW224_001485"
     CDS             9488..9850
                     /locus_tag="PW224_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL2908177.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10037..10894)
                     /locus_tag="PW224_001486"
     CDS             complement(10037..10894)
                     /locus_tag="PW224_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2908178.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11014..11925
                     /locus_tag="PW224_001487"
     CDS             11014..11925
                     /locus_tag="PW224_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2908179.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11932..13578
                     /gene="mdcA"
                     /locus_tag="PW224_001488"
     CDS             11932..13578
                     /gene="mdcA"
                     /locus_tag="PW224_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL2908180.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13680..13979
                     /locus_tag="PW224_001489"
     CDS             13680..13979
                     /locus_tag="PW224_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL2908181.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            13969..15645
                     /gene="mdcD"
                     /locus_tag="PW224_001490"
     CDS             13969..15645
                     /gene="mdcD"
                     /locus_tag="PW224_001490"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL2908182.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15649..16242
                     /locus_tag="PW224_001491"
     CDS             15649..16242
                     /locus_tag="PW224_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL2908183.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16266..17342
                     /locus_tag="PW224_001492"
     CDS             16266..17342
                     /locus_tag="PW224_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL2908184.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17367..18236
                     /gene="mdcB"
                     /locus_tag="PW224_001493"
     CDS             17367..18236
                     /gene="mdcB"
                     /locus_tag="PW224_001493"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL2908185.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18347..19111
                     /locus_tag="PW224_001494"
     CDS             18347..19111
                     /locus_tag="PW224_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL2908186.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19600..21693
                     /locus_tag="PW224_001495"
     CDS             19600..21693
                     /locus_tag="PW224_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2908187.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21718..22020
                     /locus_tag="PW224_001496"
     CDS             21718..22020
                     /locus_tag="PW224_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2908188.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            22047..23336
                     /locus_tag="PW224_001497"
     CDS             22047..23336
                     /locus_tag="PW224_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2908189.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            23355..23762
                     /locus_tag="PW224_001498"
     CDS             23355..23762
                     /locus_tag="PW224_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2908190.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            23785..24654
                     /locus_tag="PW224_001499"
     CDS             23785..24654
                     /locus_tag="PW224_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL2908191.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            24656..25276
                     /locus_tag="PW224_001500"
     CDS             24656..25276
                     /locus_tag="PW224_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2908192.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            25726..26331
                     /locus_tag="PW224_001501"
     CDS             25726..26331
                     /locus_tag="PW224_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2908193.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            26466..26918
                     /locus_tag="PW224_001502"
     CDS             26466..26918
                     /locus_tag="PW224_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2908194.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            26955..27452
                     /locus_tag="PW224_001503"
     CDS             26955..27452
                     /locus_tag="PW224_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2908195.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            27470..28180
                     /locus_tag="PW224_001504"
     CDS             27470..28180
                     /locus_tag="PW224_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908196.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
BASE COUNT         9278 a         4833 c         6014 g         8088 t
ORIGIN      
        1 gatttgatgt tataaaaaaa aaattctcat ttttggcgct atagtgaaga ggtgttacag
       61 ataaacacat gtatctgcaa gacattgacc aacaaaatat ggagggaaga aaaatgaaag
      121 atatggatat caaggccgtc tttataggcg acaaagctga aaatggacca gtttataaaa
      181 tgttgttgaa caagatggta gatgaacatc ttggatggag agaaaattat attccttcag
      241 atatgcctgc aattagcgaa ggagataagc tcactccaga ttatctagcc acccgcgacc
      301 atatgataga ggtcttggat gaagtcagtc agcgattacg cgcaggatcg atcccatggc
      361 attcagcagg ccgttactgg ggacaaatga atgctgaaac attgatgcct gctttattag
      421 cttacaacta tgcgatgctg tggaacccaa ataatgttgc attggaatct tctatggcaa
      481 cttcgcaaat ggaagcagaa gtaggacagg attttgcttc attattcaat atgacagacg
      541 gatggggaca cattgcagca gatggttcta ttgctaatct tgaaggatta tggtacgccc
      601 gttgcatcaa atcgattcct ttagctgtca aggaagtgct gcctgaaaaa gtgaaaaaaa
      661 tgtctgagtg ggaacttttg aatttatctg ttgaagaaat tttggaaatg accgaaagct
      721 ttacggacga agaaatggat gaggtcaaag cagcttcttc ccgtagcgga aaaaatatcc
      781 aaagattagg gaaatggctg gtccctcaaa caaaacacta ttcttggatg aaggcgttag
      841 atatttgcgg agtcggctta gatcagatgg ttgctatccc agttcaagag gattatcgaa
      901 tggatattaa tgctttggaa aaaacaattc gtgaattagc tggacaaaaa atcccaattc
      961 taggtgtggt tgctgtcgta ggaacgacag aagaaggtca agtagacagt gtagataaaa
     1021 tcgtccaatt aagagaaagg ttaaaagatg aagggatcta tttctattta catgttgatg
     1081 ccgcatatgg cggctatgcg cgttcactgt ttctaaacga agcaggagaa tttgtgccgt
     1141 atgcttcctt agctgaattc tttgaagaac atcatgtttt ccaccactgc gtaacgattg
     1201 acaaagaggt atatgaaggg ttcagagcta tttcagaagc ggattctgtc acgatagatc
     1261 ctcataagat gggctatgtc ccttatgctg ctggaggaat cgtaatcaaa cataaaaata
     1321 tgagaaatat catttcttat ttcgcgccat atgtgtttga aaaatcagta aaggctccag
     1381 atatgttagg agcttatatt ttggaaggat caaaagctgg agcaaccgcc gccgcagtct
     1441 ggacagcaca tcgtgtattg ccattgaatg tgaccggata tggtcaattg attggggctt
     1501 caatcgaggc agctcaaaga ttcagagaat ttctcgatca tctgactttt actgttaaag
     1561 gaaaaacaat cgaagtctat ccattgaatc atcctgattt taatatggtc aattgggtat
     1621 tcaaagaaca aggctgtaca gatttgaacg ctatcaatga attaaatgaa aaaatgttcg
     1681 atcggtcttc ttatatggat ggcgatgttt atggtgaacg gtttattact tctcatacta
     1741 catttacaca agaagattac ggtgattctc cgattcgttt tgttgaaaga atgggtctaa
     1801 cgaaagaaga atggaaaaaa gaacagaaaa tcactctgct acgtgcggct atcatgactc
     1861 cttatttgaa tgatgatcga attttcaatt tttatacaaa aaaaatcgcc aaagcaatgg
     1921 aaaagaaact aaacgaaatc atccaataga actgattttt gtgtgaaaga ataacaagag
     1981 acatatggag aacagacaat tatttgcttc atcaaaacaa ttgtatttcc ccctgttttt
     2041 catatattcc tcgccaagat attctttttt ataaaacgtt ctataagttg gatcgttcgt
     2101 ttcatgtatc aaacccagtt cactaagatc gttcaatgga tgtttcggtg atactgggtt
     2161 ttgtactgaa tcgatgaaac cttgtcattt ctgttcttga acaaaaataa aagaaaacaa
     2221 aaacatatgt cgctattgaa agaaggaata aagatgaaag atactggttc atcaaagcaa
     2281 atcacgacca tgacctttat cggaatgacc tgcgcccttg tagcaagtgt acgaaatatt
     2341 ccagatgtag cagcaacgag ctggacgatg attttttaca tgctggttgc agtattattg
     2401 tacgcttttc caatctcatt gatctctggt gaatttgctg gaatgtttcc ccaaaaaggt
     2461 ggtccagaac tttgggtatc gaatgctcta ggaaaaaaat gggggttcgt tgtctcatgg
     2521 ctgttatggg tccaaatgtt tcctgggatg gtgatggtcg cttcagcact cgctccgttg
     2581 tttggtaata ttatcgataa tgtgccgctt ggtttgaaca gcaaatttac attagttgtc
     2641 attttagtcg tttattggat catcactttt ttgaatttga aatttgatat ggcaaaaatc
     2701 ggcggaaaag tcggtgtttg gttaggattg tacgtgcctt tgacgatgat gctcttatta
     2761 gggtttgctg cgtggatcaa agttgggatc gtaccaaccg gaacattagg cagtttttca
     2821 tgggacaaat taataccaga tactacgact gcatcctcat ttgtttattt tgcgccgatc
     2881 atgtttattt ttactggtat cgaaatgtcc tctgtgtata tcactcgatt ggaaaatcca
     2941 gttaaaacgt atattcgcgg agtatttgcc gcgttaattt ttatattttt cgtcaatatt
     3001 ttgaatgctt tagttgtagc aaatgtcgta ccaaaaggac aaatggaatt gaataatatc
     3061 gcgcaatcta tttcgattta ttgccaaatt cttggtttgc cacatcttat tgtgaatctg
     3121 tttagtttac ttgtcttcat tggtgtagca gtccaattat ctgcttgggc ctcaggacca
     3181 gcaaagacag tgacagaaag tgctagaaaa ggtgcgtatc caccaaaatt caatttttgg
     3241 aagacgaatc aatttgacgt atccaaatcc gttattttga ctcagtctgt cattatctca
     3301 atctttgcac ttttttattt gctgattcct ggcgtcaatc aagcattttt gatgctcgta
     3361 aattcaacta ctgttatcta ctgtatcgtc tatgtcatca tggggatcgc agttttgaga
     3421 ttgcgttata cacatgcaaa gttgaatcga ccatttagaa ttggaaaaaa agagaaaaag
     3481 agcaatcttg gagtttggac tgtcgttatt gttttatttg cggcaatcgg attttctgtc
     3541 ggactgacga tgaaagctgg aacatggatc aatctaattg cagtcacagc catcagtgtc
     3601 attttgtttg tagtaccact ctgtattgaa aaaataaaga aaccttcttg ggaacaggaa
     3661 gtcgccatgc tgcttgaaaa agaaaaaggt gaacttgatg gaaaaaccgg caatgaaacg
     3721 gactctaact aatcgaaata tccagttaat cgcattaggt ggcgcaattg gaacaggatt
     3781 atttttaggt tcagcaaagg caattgcatc agcagggcca gctgttattc tcgtttattt
     3841 gatatgcggt ttggctattt atggtttgat gcgggccatg ggtgatttat ttttagcaaa
     3901 tagtacattt aactctatca gtgatttcgt tgactattat ttaggcaaaa agtgggcatt
     3961 ttttgtcaat tggacttatt ggctttgctg gcttggagct ggacttgcgg aattaacagc
     4021 aataggattg tatatccatt tctggtttgt acatgtacca gctttgataa gcggaggtag
     4081 cgcactaatg atcttaatgg cagtcaacct tgttgcagtc aagtggtttg gtgaattgga
     4141 atcttttttc tcaatcatca agatcttggg gattttcctt tttatcttat tgggtatagg
     4201 attgagtgtc tcagattttc atacccagtc tatcggaatc aaagaaaatt ttggacagat
     4261 cgatgaatta ctaccgtttg gctttgctgg aatggtagtc tcatttccta tggttttatt
     4321 cagttttgct ggaattgaaa tgatcggctt aaccgttggc gagacaagtg atccgcaaaa
     4381 gaatttgaaa aaagcaatca actgtctgcc ttggcgtatt ctctttttct atataggaac
     4441 gattttgtcg attttatttg ttgttccatg gcattgttta gatttaaaag aaagtccttt
     4501 agtgactatg ctgcaagtga tccattgcca aggcggagca gcaattgtca atctaatgat
     4561 tttggttgct tctttgtcct cagcaaatag tgcagtctac agtactagca ggattttata
     4621 tcaaacagca aaagaaaaaa gtttgcccca ttggttttgt gcgctttcta agaaaggcgt
     4681 acctttacac ggtattttaa ttacaggaat gttatttttt tgccggtctt tgtctacatt
     4741 tttttgtagc tgatagtcga ttgttttttc agcttttggc aggcatgaca acgagttgtt
     4801 ttctatttgt gtggtcttcg atcttgtgtg cacatgttca atttattaaa gaaaagaaac
     4861 ggaacactac acaaagatat aaagatcttg ctttgttggt gttttttact tcaattgctt
     4921 tcagcttgtt attcgaaagc acgacacgct ttattccttt tttattggtt atctggtttg
     4981 tattcttaaa ttttgtatat aaaagaataa gcaatagaaa tatcagttag gcccagatac
     5041 attttgcaaa acgaattatt tcagaccccc ctgaaataat cgatacgcct gaaaaaactt
     5101 ccctgcgtat aataacagta tcaattaaac aggaggggat aggatgaact ggtacagatt
     5161 gacggtagaa gatgtattga aaaactatca aacatcaaca gaaggcttgt cgcaagaaga
     5221 acgacagaaa cgcctgactg atcaaggata taatgaaata aaagcaaggc agcagacaaa
     5281 acgatggaaa aaaatagcaa agcattttac tgatctgctg atgatcgtgt tgatcattgc
     5341 ctcttttttg aaatttgctt ctagtgaata tattgaagga agtattattt tattcgtagt
     5401 gatcgtcaat ggactcgtta gttactggca agaaagaaaa gcggaagaat cattgaatgg
     5461 tttgaagcag ctgatgggac aagaagcgat agtactatca aacgggatgc aagaaaaaat
     5521 ccctgcgaaa gaactggtaa aaggagatgt cattgtctta cgtgccggag atgtcgttcc
     5581 agcagatatc cgattaatcg aagcacatga tttattagtg gaagaatcga ttttgacggg
     5641 agaatcagaa gcatctgaaa aaagtcatgc cgttttgaca gaagaagaat cgatcggcga
     5701 ccagaaaaat atgggttttt caggaacgct tgtacaatct ggttctgcag tcggtgtcgt
     5761 tgtagaaaca ggaaacacga cagaaatcgg taaaatcaat caagcattgc aatcggtcga
     5821 acaacaaacg acgcctctga ttaaaaaaat caaccgatta aacaaacaaa tttttcaagg
     5881 cattctttgt ttgagcctat tcttagttat ttttacgaca tttcgatatg gtatggattg
     5941 gcatattctt ctttcggcta cgattgcttt ggtggtgtca atggtacccg aaggattgcc
     6001 agctgttttg accatgattc tatccgttgg agtacatgaa atggcaaaag aaaaggcgat
     6061 catcaaagga cttccttcgg tagaaacatt aggttctatg acagtgatct gttcagataa
     6121 gacaggaaca ttgacaaaaa atgaaatgac agttgtggat gtagctgcaa aagaagaagc
     6181 ctgcgtgttg tccattatga aaaactgtca ggaattaaaa acaaaagaag aacagaaaac
     6241 agaaaattta agtggcaatc cgacagaagt tgcgctgatt cgacacacag aaacagccaa
     6301 tctacctttg aaaaaaatag aagcaaagat tccgttcagt tctgaataca aatatatggc
     6361 aacgatgcat gcggaggaaa aaggagcggt gatttacgtc aaaggtgcac cagaagtatt
     6421 atttgctaaa tcaactcttt cttctgcaga acaagaagca tggagtcaga cagctgcaga
     6481 atttgcgaga aaaggtcagc gtgttttagg atttgcttat aaaaaagtag attcgaaaca
     6541 ggaactcacg catgagactt taacccaatt aacttttgca gggatcgccg gattgatcga
     6601 tccgccaaaa gaaagtgcag tcaaagctgt aaaggaatgc caacaagcag ggatctcagt
     6661 gaaaatgatt acaggggatc acaaagacac agcaaaagcg attgcggaac aaattggctt
     6721 aaaacatact gccaaagttt tagaagggat cgatttggat cttatgtctg atgaagagtt
     6781 gattcagcaa gtgccaatag tcgatgtctt tgctcgaacg acaccagaac ataaactgcg
     6841 aattgttaaa gctttgcaga aaaatggaga aatcgtcgga atgaccggag acggcgtaaa
     6901 tgatgcacct gctttgaaaa gatccgatgt aggaattgct atgggaatca aaggtagtga
     6961 agtaagcaaa caggcagctg atatggtttt aggagatgat aatttccata cgattgcaaa
     7021 agccgtaaaa gagggccggc ggattttgga taatttgcaa aaaaccatca atttcttctt
     7081 gccaactgca ttagctcaag gattgatctt gatttgggca cttatgctga atcgcccgct
     7141 acctttgtcc ccagtacaga ttttatgggt caatatggta acgacaatca ctttatctta
     7201 tgcactaggg ttcgagtcgc cagatccaga aatcatgaag cgcccaccaa gagatccaaa
     7261 gcaaggaatc ctatctggtt atcatttgtt tcggatcatc tatgtctccc tactgatcat
     7321 ggttcctgcc tatttattgg ctatgcagtt tgaagggcaa agcttacagc aaaccattct
     7381 tctgcaaaat atcgtcttag ctcaagctgt ttacatgatc aattgcagag agttgtccaa
     7441 gccgtcattg aaccaaggtc ttttgcaaaa caaagcgctt tttctttcat taggtatcct
     7501 tgctttattg caagggatgg ttatttttct accagtcggc cagcagctga tcggtacagt
     7561 aagtttaacg atgacacaac agttattgat tggagcaaat gtgattcttc tattcttagt
     7621 tgtagaaata gaaaaaggga tcatgaacaa actgtttcgc aaaaaagaaa aaacagctag
     7681 agaatacagt cgctagccgt tactataaaa aagttttgaa agtgagttca gtcaaacgat
     7741 tgagctcatt tttcgttgga atagaagaaa tgtatacaat agggatatct agagaatcta
     7801 gagggaaatt ggaaatcaag atatctgttt tttcaaaaga aatagtggaa agatcggtta
     7861 gttcgatagg taagaagcag acacgttttc ctaagtaatc ttttaattcc tgctgcaaga
     7921 acgccttcca tgaaggttct ccttggaaaa taaaatagac attgaacaaa ttcggtttga
     7981 tagaaaaaat cgtttgttgg atcagtagag acaagagttg gagaaagtaa tttggttcat
     8041 tgatttctat ttcttttttt agatcgttca gtaaattctc tttctgttga tacaagtgtg
     8101 catattgctg atcatatgtt tctaaaaaac gatggtattg acggtccaat gcttcaatca
     8161 atatcggcga ttcatgatat tttaatatga ggagagccaa gttatcagtc agagatgttt
     8221 tttttagttg atcaagagac agctctgaag ctagttcctc tacaaacgtt tcgaggacct
     8281 ctaaaaatgg agaatgatgc aggcagcgag cggtttcttg tccatagtta ttgttgtaat
     8341 tccaactttc cagaaagcag aaagaagcaa aagaaagctc agtgtcttta agataaatac
     8401 cttccgttaa atacaatttt ttcaatgcag agtcatacag ggaggagagt gtgctgttgg
     8461 tatggataga agaatctaat cggcaattcg cttttatacg gatcgtattg ataaagaacc
     8521 aacatgttcc gaaaatctct tctgtttcaa ccgctagttc agtttgactg aggtttctta
     8581 aaaattgaaa ataatgggat tcatggatcg aataatcctc gaaaaaatag ttgttatgag
     8641 tgaaaggaat cagcaatcga tgataaaaag tacgaatcgc acgctctttt ccaagaatac
     8701 aagctgtata tttcgtcaaa tcgatctgca agtcgtaact tgctaaacgc tgattgattt
     8761 tagtcacatg tcttttcaat gtcccataag aaagcccatg gtcggctaga aaatcaggga
     8821 cagcaacttt ttttgtaaaa agcagatttt tcagcagttc gacactgact gattgtttga
     8881 aaaaaatctc ccaaaggtca tttgttaata aattttgctg gttttgcaaa ctgattcctg
     8941 cttgatcgct gatgatgtcc cagccttcgg gcagctgaga gcgaatagat tgaaggtcag
     9001 aaaaaatcgt tcgttcggtt gtccctatga tttcagctaa ttcatttacg gttatctttg
     9061 tatgattcag caattgttct actaaagaaa catgacgtcg aatatctttt tcggtaatga
     9121 tttttttcat tagtgaatac atcaatcaga ctcccttcta ttctaagaat acggagaaaa
     9181 taaacggtgg tcaagtttca actgattttt caatcattgt ttatttttct ggagcattta
     9241 ctgatgataa gaatttcatc atgaacttga aaaatgatac cactaaaatc aaaaaatagg
     9301 tgggcaaaca ataataaaca ttttaatagt agatggtagc aagaccaaca caggcggatt
     9361 gaacatttaa gagttgataa aaaagaaaaa tatcgaagta tttgattagc aactacttgt
     9421 attcattttt ctattcttac gtggtatctt tgaataaagc gaatcaataa gaaagagatg
     9481 gaagcagatg ctaacgaaaa aagaactaat cgatcacatc aatgaaacat acaatttgtc
     9541 tccagaatat atttttaaaa aatttcctga ttattgcgta ttcagacatc aaaaaagcaa
     9601 aaaatggttt ggggtcctga tgtctgttcc agaagaaaaa atctatgatg atgcaagtga
     9661 ccataggatc atcaatattc ttgatttgaa ggtcgatcct gaacttggcg gaattttgcg
     9721 aggaaaagag gggtattttc cagcctatca catgaataaa gaacattgga tctctattga
     9781 tctacgtaaa atagaaaagc tagatgaact agcagatttg attgatggaa gttttaaatc
     9841 aacgcaatga atgtctagga caatagagaa gaggttgtga cagaaatgtt tagcttcaag
     9901 aaataagaag gaatttacga aaattgcttc tcaaattttt gggaaatttc ggcttatttt
     9961 ctaagaaatt acttctggaa caccgtttgt tcgaaaaaag agctgcgaca agaattttgt
    10021 cccagctctt ttttttttat tttcctaaat aaaattcccg catcgttttt gcaatcattt
    10081 tgatcaaagg atgatcttct gtgaagctag aagataaata aattgttcgt gtcaagggca
    10141 aatctttgat ttcccgcaag acgaaaggat catttttatc gtaagaccaa gaaacttttg
    10201 ggaaaaagct aacagctaat ccttgcttga caatagaacg taatgtcgca ggatcatctg
    10261 tcgtcgaact gatcgtcaat tcgatacttt tcgcttcaag tgctgcatcc agtgtatgac
    10321 ggaaggcgtt gctctttgac agcatcaaga tgggggtgcg ttggatatct tctaattgca
    10381 gatgttcttt tttagcaaga ggatgtttct taggaatggc gacataaaca ggttctttca
    10441 tgataggaat attggtcatg ccaggcttca tctccgaagt gatgtacagt acaggatcgg
    10501 taatatcatc tggtaaatga tgttgcatga tcgttaattg gatatcagga tgcttttcca
    10561 taaaaattcg gaccaagttc ggaatagaaa cagaggctac ctctaaatac aatatgacgg
    10621 aagtaacgat cttctcattg accgaagaca ataatttgat tccttcttct aatgtgtcta
    10681 atgtcttgac tgcatatgga taaaattctt ctgcataaac ggtcgtctca atgtttctgc
    10741 cgctatggta aaacaaaggg acacctaatt cgttttccaa atgtttgatc gcacggctca
    10801 aggcaggttg agaaataaat aattctttgg ctgcttgggt aacatttttt gtttcgaata
    10861 cttttttaaa atactgcaga tgaagcaatt tcataattat ccatcccatc catacctttt
    10921 tgttatggtt ttttcttttt ttggcatttt ttattatagc agaaattgac taagatagca
    10981 gtggaaaaag taagtgctga ggaggcgagg ctcatgagta caggtttctt atttcctgga
    11041 caaggcagtc agaagttagg gatgttagcg gatatccctg aaatgtacat ggaacgttta
    11101 gaaaatgtga cggggtatcg gttagttgat aaagaagaaa actatcaaga tacggttttt
    11161 atccagctag cattactgac aaaagcagta ttttatttag atgaaatgga aaaaaacggt
    11221 attcagcctg atattgcagc aggacattcg attggtgctt tttgtgcagc tgttgcttgt
    11281 cgatctttga gatttgagaa tgcagcggaa ctagtctatc accgcgcagt cttaatgaaa
    11341 gaagcttatc caagcggtta tgcaatgggc gtagtagtag gtgtgacgag aagcgaagct
    11401 gaggaaattg tagcaaaaac atttgatcct gaccatccag tttatctgtc aaatgaaaat
    11461 tgtccgatgc aacatacgtt atctgggagt atcgaaggtt tatccgttac gttggcagaa
    11521 gccaaacggc accaagcgca aacagcgaag ctgctgaaag tccctgtgcc atctcactgt
    11581 ctcttgatga atgatacggt caagaaattc aagccgtata tgcagtctgt ccatctatct
    11641 gatgctagat gtttgtatat aaaaaatacg gatggaagag ccactcagga cgcagaagag
    11701 attcgtaaag atttgttgga aaacctagct ttccctgtgc agtggaacca aatgatggac
    11761 ttggcaaaag aattaggaat ggatctgacg attgaatttc cccccggcaa tactttgaca
    11821 aagctgattc atacaaaatt tggtgtagat aaagggatcc gtacgatcaa tctagaccaa
    11881 catggtattg atgacgccac gtttctatat caaaaatgga gatgactaat tatggaaaaa
    11941 ccaaaccgca actgggcttc taaacgcgaa aaaaaagcgc agcgattgga aaaaatcagc
    12001 aacttgctca atggcaagta tgtagaaact gaaaaaatcg tagaagtaat ggaaaaactg
    12061 attgctcccg gcgacaaagt tgtattagaa ggcgacaatc aaaaacaagc aagttttctt
    12121 tcagaaagtc ttgaaaaagt agatgcagaa aaagtgaatg atctgcattt gatcatgtct
    12181 agtatctcac gaccagaaca tttgaatatt tttgaaaaag gaatcgcaag caagatcgat
    12241 ttctcttatg ctggtgcaca aagcttgcgt gtcgcccaaa tgattgaaga cggcacaatg
    12301 aaattaggtg atatccatac ttatttggaa ctttatggac gcttattcat cgatttgatt
    12361 ccaaatgttg ttttagtagc tgcagataaa gcagataaag acggcaactt gtacacaggt
    12421 ttcaatacgg aagaaacacc aacaatcatc gaagctgcag ctttcaaaga tggtatcgtg
    12481 atcgttcaag ttaacgaagt tgtcgattct ttaccacgtg tagatatccc tggtgactgg
    12541 gtagacgttg tcgtgaaagc agataaacct tatcaattag aagctttatt cacacgagat
    12601 cctcaaaaca tcacagaatt gcaaattcta atggcaatga tggcaatcaa aggaatctac
    12661 gcagaacacg gcgtacaatc tttgaatcac ggtattggat tcaatacagc agctatcgaa
    12721 ttgttactgc caacttatgg tgaatcacta gggttaaaag ggaaaatcgc gaagaactgg
    12781 gtattgaacc cacatccaac gatgattcca gccatcgaat caggttgggt tgaaagcatc
    12841 catagttttg gtggcgaagt tggtatggaa aaatatattg ctgctcgccc agatgtcttt
    12901 tttgtcggta aagacggcac gatgcgcagt aaccgaacat tatcacaagc tgcaggacaa
    12961 tatgccatcg atatgtttat cggttcaacc ttgcaattag attacgaagg aaactcttct
    13021 accgtaacaa aaggacgttt atctggtttt ggcggtgcgc ctaacatggg acataatcct
    13081 ggcggtcgtc gtcattctac accagcatgg caatcacttc gcgacaatga tgatccatta
    13141 gggaaaggcc aaaaattagt tgttcaaatg gttgaaacat atggttcaaa taaaaaacca
    13201 gtctttgtcg aagaattaga tgcactagct gttcaaaaac aagcaggctt agccaatgca
    13261 ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg aattgcttat
    13321 ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat tggcggtgtt
    13381 acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc agccggcatc
    13441 gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc attattagct
    13501 gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc accaatgaaa
    13561 ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta cccagatgga
    13621 agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg gagagatcca
    13681 tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa atccatgtag
    13741 gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa acagaatcta
    13801 ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat gtattaacga
    13861 gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc ggtgctacac
    13921 caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat gaaaaatagt
    13981 tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg acgtgaattg
    14041 attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat cgtccctgaa
    14101 agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc agtagtaatc
    14161 gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc aaaaatcgtt
    14221 gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt tccagtcatt
    14281 gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt atctatttca
    14341 gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg tttgattcca
    14401 ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag ttatttaatc
    14461 gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca agaagcaggc
    14521 gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg atctcgtcaa
    14581 cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc aatcaaagct
    14641 gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag ctcagcattt
    14701 tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga cgattacaat
    14761 gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc agaaggaaat
    14821 gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg catgaaaaat
    14881 ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat agaagaagcc
    14941 atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg aaacggtgaa
    15001 gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta cgaagaagat
    15061 aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag ccaagcatac
    15121 ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc tgctgcttat
    15181 gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa tgctatttca
    15241 ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa caataaagaa
    15301 attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg ttcggttgct
    15361 gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa taattataca
    15421 aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc ttcagaacaa
    15481 aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg caatacaaat
    15541 gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg acgtgtcgaa
    15601 acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat gattcaagca
    15661 catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc tgaatgggtc
    15721 gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca cgcagaaata
    15781 ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga aatctcaaaa
    15841 catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc gtatagacaa
    15901 gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa catattatca
    15961 ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg gattccggca
    16021 gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat tgaattgcca
    16081 attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca agtagtgact
    16141 cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa aattctattg
    16201 aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa aagagagaga
    16261 aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag aaagtatcag
    16321 ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac agccacagcg
    16381 caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca gcaccaatgt
    16441 ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct tattgggaat
    16501 ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg acggcttatc
    16561 tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca agctttttac
    16621 tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg atgctgcaga
    16681 tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct aataaaaaat
    16741 atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat ctgttctatc
    16801 aaacacaagt attgactggg ccttatcatg acagctggta cttattagat cggtttgttg
    16861 ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc tatcaatatt
    16921 tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc ttttctgtcc
    16981 gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac ttgaatacga
    17041 tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct tccaaaatga
    17101 ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat gcttatcgta
    17161 cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa gatagttggc
    17221 tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca agttgtgcat
    17281 ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt ttcagcaagt
    17341 aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa gcagtgactc
    17401 agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta agtccaaaac
    17461 ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat gctttatttc
    17521 ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata gcatggcatc
    17581 atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa gcggaaaagg
    17641 caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg gcaatggggc
    17701 tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct ttatcgaaaa
    17761 tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt cataaaaaaa
    17821 ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat gaagaagcga
    17881 tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta gataatgatt
    17941 catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta gatgacacat
    18001 gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt gctcatgctg
    18061 ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt tgtcaggaaa
    18121 accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc atgctgaaga
    18181 tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat gactaaataa
    18241 gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct tttagcaagg
    18301 ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga aaaaaatcaa
    18361 ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga cccaaggatt
    18421 cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg gcagaggttc
    18481 atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg gcagagattc
    18541 ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg gatttcttgc
    18601 attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga ataaaagaca
    18661 aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta aagaaaagaa
    18721 agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt gggaagaaga
    18781 aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac atcagaaagt
    18841 tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa ctattcagga
    18901 agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg atttcagatg
    18961 ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta agacacaacg
    19021 acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa gatggcaagc
    19081 cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg gagttgtgac
    19141 aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata gcctatcggc
    19201 aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt cttatcgctc
    19261 aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc ttgcatctgc
    19321 gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt tgttcttatt
    19381 acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca aaatttttgt
    19441 aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct tattttttgt
    19501 ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt tattatgtaa
    19561 gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag aacaattgaa
    19621 atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct agcaagtgag
    19681 ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat ctggttgagc
    19741 agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt gaagctggaa
    19801 aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg ctttttgaat
    19861 cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat cccgatccat
    19921 ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga agatatgaga
    19981 cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg aaagcaagga
    20041 ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt tatcaatcag
    20101 aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc ggctcaaaaa
    20161 atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat ctattccaat
    20221 aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt tacattgatc
    20281 tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa aaatacagaa
    20341 ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt tcaattggac
    20401 aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt caatgctaga
    20461 gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca attgatccaa
    20521 tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt attcgaaaat
    20581 ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca agtcatgaat
    20641 ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt cactcatttt
    20701 gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat agcttttctt
    20761 gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta tgtttataaa
    20821 tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga gaatctgaaa
    20881 gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat cgaattagta
    20941 gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg aaataagccg
    21001 cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga agactttttg
    21061 aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac ggagttattc
    21121 actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat catctatggc
    21181 aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga gatccagcct
    21241 atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc cactacttgg
    21301 gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt cgaagagcgc
    21361 tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt gattggcaaa
    21421 cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg agtcagcgta
    21481 gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt aaagattatt
    21541 ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga actaattgaa
    21601 ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat cgaagatttc
    21661 aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag aacaaagatg
    21721 aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt tgcagcacag
    21781 atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga ccattgtgat
    21841 atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa ttttcgttcg
    21901 cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa aaatattgtt
    21961 tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg agttatctaa
    22021 atacaaaaag aaaagagggt aaaaacatgg gcgttatcaa ttttatcatc gaaaatattt
    22081 tgactcaggc atcgatcacc attgcattga ttgctatgtt gggtctacta ctgcagaaga
    22141 aatcagcagg acaagtcatc tctggcacgt taaagacact cctaggattt caagtgttga
    22201 gtgccggttc gagcatcatc gttggaagtt taacttattt cgggaaaatc tttactgaag
    22261 gatttcatat gcaagggatc atcccttcta ttgaatctat caatggtcaa gcaatgaatg
    22321 atttgggttt aggtagagat attgcactga cgttcttagc catttttgta ttcaatatta
    22381 ttttggctcg tttcacaaaa tggaagtaca tttttttaac tggtcaagca attttatgga
    22441 tggcaacgat gacgacagtc tttggctatt ttgccggtct gcgagggatc gtattgatct
    22501 tggtcggtgg ttttatcgga gcttgtttcg ccattgctat gccggcagta gcgcaaccga
    22561 tcattcgtaa aatcactgga tcaaatgata ttgctttagg tcatttttgt acgatcgggt
    22621 acttatttga agccggtgtt gctaagcttt ttggtgagaa aggcgaaaat aaaaagtcga
    22681 tcgaagatat caagttgccg acacatttcg aatttctgca agatacttat ttgtctgtca
    22741 tggtcgtgat ggtcccgcta tatatcatca ctgttttatt tgcaggcgaa ccattcgctt
    22801 ctgaattatc aggagatcaa aactatatca tgtttgcctt tttacaagcc atccaattcg
    22861 tagtgggtgt gtatgtattg ctagctggtg tacgtctact gttaggtgaa atcgtgccgg
    22921 catttcgagg gatagctatg aaattagttc ctgatgcgat ccctgcctta gattgtccag
    22981 ttttctttcc ttatagccca aatgctgtca ttttaggttt cattacgaca acgattggta
    23041 cgattattgc catgtttact ttgcccatgt tcggcttagc gatgatttta cctggtatgc
    23101 taaccaattt ctttgccgga gggacagcag ggatatttgg caacgcagtt ggcggacgcc
    23161 gaggagcgat cattggcggt attgctcatg gtttctttat tacattattg cccgctttgc
    23221 tagtaacgat ctttaattcg atgggattca acaatgctac agcaaccgat gtagatacag
    23281 tagccgcagc actcttatat gcctggatct taagcccagt tttgaaagca ttttaattga
    23341 aagtagggaa aatgatgttt ggatttttta agaaaaaaga aaaagaaccg aaaaaagttc
    23401 tggcaaaaga agaaaaagcc ctatcgccag aagcgaccga aaagattcaa tcagagatta
    23461 cacaattgaa acaagagatt tccactaccc aagataagca taaattagcg aaactttatg
    23521 aacaagttgg acttaaattt tctgaattgt atgtgaacga tcaagccatc caatatttag
    23581 aaaagagttt ggaaaataaa cagacgatcg gagatgggta taaaaaactg atgagcctct
    23641 ataaccagaa gcgtgcagat gctgctcgtg ctggtgatga tcaagggatc gattactaca
    23701 tgggcaaaat ggatgagatg cgacaaattg caaaacaagt aacgatcaaa ggaaacaaat
    23761 aaaaagataa ggtggcaata aaagatgtat acaacattga aagaagtaac aaaaacagca
    23821 gaagagttga attttacagt tggagctttt aatacgcata acctagaaat gcttcctgct
    23881 atgctaggcg cagcaaaaga aatgggttcg ccgatcatta ttcaaacaag tgtcgatacc
    23941 gctaaatata tcggcatgca tgtgttagtc aatgtaatga cagctattgc agaagaagaa
    24001 atggttgatg cagtcttgca cttagaccac gcccgagact ttgatgatat caaagaagca
    24061 atcgacagcg gatatacttc tgtcatgtat gatgggtccc atctgccttt caaagaaaat
    24121 atcctgaaga caaaagcagt agtagaatat gcccatgcgc acggtgtttc cgtagaagga
    24181 gaacttggaa caatcggcgg tacagaagaa ggaatccatg tcgctgaaaa tgacaaggta
    24241 tatacaagac cggaagacgc agtgaaattt gtagaagcta caggcgtgga tgcattagct
    24301 attgctatcg gtacgaatca tggacaattc aaatcaaaaa cagaagtaaa cattcccttg
    24361 ctgaaagaaa tcgatgcagt agttgatgtc cccctagtta tccatggagg aacaggtgtg
    24421 aaagaagaag attatcctga attgatcaat aacgggatcc gcaaattcaa tgttggaacg
    24481 gagcttttag tcaactggac gaaagtagct aaagaaacct ttagtgaaac agaaatcaac
    24541 aagtctcttc gccataatgt gattccagca aatgaagcag taaaagaaat tgtcaagcat
    24601 aagattggac tgtttatgaa cttggaaagt ccaattaact taggaaataa atgagatgaa
    24661 aaagtatttc attcttctgg ctggtagtcc tggtacagga aaaacatatc ttatgaataa
    24721 actacgggaa cgatttccag atatgtatgc catcacgctc gatgaaatca aggaatacta
    24781 tgcagaatcc atcgggttta acaatttgga agaacgggcg gaacaagaaa ggacgaaagt
    24841 ttatcctttc tattacaaag cacttgattt gtatatggaa gcaggaaaga aagtgattgt
    24901 gtccgagtat ccttttagtg ataagcaaaa agcacgactc caagaattag cagatagata
    24961 cggatatcag gtgatcacga ttcgcctaac agcagacttt gaagtgttat ggcaacgtcg
    25021 gtacaaacgt gatcgtgaat cagatcgtca tttgagctat atcatggatc attatcacta
    25081 tggtgacgta ttggaagatc gaacgaaagg gaccaatcat attacgaaag aagcattcaa
    25141 aaagatcatc gccgaccgaa aatatgacaa attccagtta ggaacactat atgaatttga
    25201 tgtaacagac tatactcaag tggattacac tccactattg gataaattag aaaaagaaat
    25261 caatgacaat aagtaacaaa gaaatggctt aatatcgaaa aattggcttt tgaacgctgt
    25321 ttaccgagag gtcgtgagcc tgtcgttcag ctccgagtca caaggagcaa tttcaaaaaa
    25381 acagcttctc atgtttgatg aatattgtga cttgtgaccg aggagttggt aggagaacac
    25441 cgtttagtgg ggaggttgtg aaaaagctgt cctgcctcaa ttaataagaa cagccagaca
    25501 agaatagctc gtcatatttt tcgtttggtg gggcttatta ccgcagatgc aagcttttga
    25561 acgccattta ttcacagccc cagagaagtc gaatgtcttt tgttctcgag ttttttaaca
    25621 ttcttttatt tattgcagat ttcattatat ttatttgccg taattaaacc agtcttctat
    25681 acttgattac atagtcatac atttgctaaa aaagaaggga agagaatgaa caagaagttg
    25741 gctcaataca aaagactact cgcactttct caagctggtc tatattatgg aaaagatgta
    25801 tttgataaag agcgttttga ggagatccgg acgatttcat tggaactgat cagtgaaata
    25861 ggaaaagaaa ctttcgaaga aataaaagcg ctcacaacga ttggagaagg acatccaaca
    25921 ccaaaagtag atgttcgtgc ctatatcaaa aaagatggca aggtcctact aatcgaagat
    25981 aaacggacga aagaatggtc acttcctggt ggatttgctg aaataggatt gtctcctgaa
    26041 gaaaatgtgc gtaaagaagt ctatgaagaa acaggattaa cagtagaaac tactcagttg
    26101 cgagcagttt ttgatacgaa taaacaaaaa gatattccac agctttttca gtattataaa
    26161 cttgtttttg cttgtgcgat ccacggtgaa gaggccaagt ttattgagaa taatgaaact
    26221 tccaacatgg gattttttag tatagaagag ttgccaaagc tatcagaaaa gcgaacaacg
    26281 aagaaacagt tacttatttt agaaaacaaa aaccagatct attgtgattg agctttttcg
    26341 taaaagaaga aaagaatata ctttactagt tattaaaaaa gaacttgatt tttttattga
    26401 aaatagttgc atttgcaaca aaaagaggtg aactagtctt acaaacaaaa taaaggtggg
    26461 atgaaatggc tgaaatcctt cgagacatcg gaatgattgc ccgagcgcta gattctatta
    26521 gcaatatcga attcaaagaa gtcgatttaa caagaggaca gtatctttat ttagtacgta
    26581 tttgcgaaaa tcctggaatc atccaagaaa aactagcaga aatgatcaaa gtagatcgta
    26641 caacgactgc ccgagcaatc aagaaattag aatccaatgg gatgatcgaa cgtttggaag
    26701 ataaagaaaa taaaaaaatc aagaaactct atccgactaa aaaaggagca gaaatctatc
    26761 ctttcatcat tcgagaaaac aactactcca atgcagtcgc attaaatggt ctttctgacg
    26821 aagaagcaaa acaattggaa tatcttttaa ataaagtatg caagaatgtt agcgaagatt
    26881 ggaattttgt caaaaaaggc aataaaagga tctattgaaa ggaagaagcc gtgaataaat
    26941 acgaaattcg aaaaatgacg ccttcagatg tagacttact acaacaaatt agtatcgaaa
    27001 catttacaga tacatttgga aaagataatt caccggagca tttagctgat tacttgacac
    27061 aagcttatgc actagaaaaa cttacagaag agctaaaaga taaagaaacc gattttttcc
    27121 tagtgagtat cgatcaagag cctgcgggtt acctgaaact aaatacagga acgagtcaat
    27181 ctgaggaaat ggctgatcat tttttagaaa tcgagcgaat ctatgtacga aaaaaattta
    27241 aacatcaagg tatcggtaca tttttactag atacagctat tgaaaaagca aaagaaaagc
    27301 aaaaaacagc catttggctc ggtgtttggg aacacaacga tgttgcgctc gctttttatc
    27361 gtaaaaaagg atttatcaaa tacagcgaac atgtttttta tatgggagat gatccccaaa
    27421 cagactatct attcatcaag caattattct aatcaagagg tgtgaacaga tgaaaaaagt
    27481 aatagtggat accaatttct gggaagtatt tcctgaagca accatcgaaa tcttatccgt
    27541 aaatggtatc gataaccacg taacaaaaga aagtgaagaa acgtatcatc aattattgaa
    27601 tgaggcagca aaagaagcaa gaaattacct tacagaagaa acattcagcc aaaacgaagt
    27661 cattgctcag tggcgtcagg catttaccac attcaagact aaaaaaggtg cacgttcttc
    27721 tattgaagct ttactgaaac gtgcaaatca agaacgggaa tttttcccaa tcaatccgct
    27781 tgtagatatc tataatagta tttccttgtc ttttggcgta ccttgcggcg gagaagatat
    27841 cgaaaagatc gcaggaaatc tgcatttagg aaaagcccaa ggtggcgaat cattcctgcc
    27901 tttaggtgca gattccgatg ctccggcact tccggaagaa atcatctact atgatgaaca
    27961 aggagcgatc tgtcgctgtc tcaactggcg tgaagcccaa agaacgatgc tgacggaaga
    28021 aacacaagca gcggtgctgg tcattgaatc catcaatgct gaacaagcgg cacgtggcgc
    28081 acaggcaatc gaagcgttga aggaaaaaat cgatcaaaca tttggcgtaa ctagtgttag
    28141 acacacacta accaaagatg aatcagaagc agaattataa aagaatcgaa ataagaagag
    28201 cggcattcat tgc
//
LOCUS       DANKMN010000037        27903 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000037 DANKMN010000000
VERSION     DANKMN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            139..1878
                     /gene="yfcC"
                     /locus_tag="PW224_001505"
     CDS             139..1878
                     /gene="yfcC"
                     /locus_tag="PW224_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2908197.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2035..3219
                     /gene="iadA"
                     /locus_tag="PW224_001506"
     CDS             2035..3219
                     /gene="iadA"
                     /locus_tag="PW224_001506"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL2908198.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3330..3533
                     /locus_tag="PW224_001507"
     CDS             3330..3533
                     /locus_tag="PW224_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2908199.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3634..4296)
                     /locus_tag="PW224_001508"
     CDS             complement(3634..4296)
                     /locus_tag="PW224_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2908200.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4309..5811)
                     /locus_tag="PW224_001509"
     CDS             complement(4309..5811)
                     /locus_tag="PW224_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908201.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6359..8038
                     /locus_tag="PW224_001510"
     CDS             6359..8038
                     /locus_tag="PW224_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2908202.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8165..8644
                     /locus_tag="PW224_001511"
     CDS             8165..8644
                     /locus_tag="PW224_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL2908203.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8863..9543
                     /locus_tag="PW224_001512"
     CDS             8863..9543
                     /locus_tag="PW224_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL2908204.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9551..10882
                     /locus_tag="PW224_001513"
     CDS             9551..10882
                     /locus_tag="PW224_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2908205.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10897..11859
                     /locus_tag="PW224_001514"
     CDS             10897..11859
                     /locus_tag="PW224_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL2908206.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11970..12308
                     /locus_tag="PW224_001515"
     CDS             11970..12308
                     /locus_tag="PW224_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL2908207.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12387..13094
                     /locus_tag="PW224_001516"
     CDS             12387..13094
                     /locus_tag="PW224_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL2908208.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13197..14678)
                     /locus_tag="PW224_001517"
     CDS             complement(13197..14678)
                     /locus_tag="PW224_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908209.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            14987..15523
                     /locus_tag="PW224_001518"
     CDS             14987..15523
                     /locus_tag="PW224_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2908210.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15601..16971
                     /locus_tag="PW224_001519"
     CDS             15601..16971
                     /locus_tag="PW224_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL2908211.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17038..17373
                     /locus_tag="PW224_001520"
     CDS             17038..17373
                     /locus_tag="PW224_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908212.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17454..18311)
                     /locus_tag="PW224_001521"
     CDS             complement(17454..18311)
                     /locus_tag="PW224_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908213.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18418..18933
                     /gene="tadA"
                     /locus_tag="PW224_001522"
     CDS             18418..18933
                     /gene="tadA"
                     /locus_tag="PW224_001522"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL2908214.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18956..19040
                     /gene="ffs"
                     /locus_tag="PW224_001523"
     ncRNA           18956..19040
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PW224_001523"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19250..20635
                     /locus_tag="PW224_001524"
     CDS             19250..20635
                     /locus_tag="PW224_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2908215.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20672..21349
                     /locus_tag="PW224_001525"
     CDS             20672..21349
                     /locus_tag="PW224_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL2908216.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21357..22121
                     /locus_tag="PW224_001526"
     CDS             21357..22121
                     /locus_tag="PW224_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL2908217.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22123..22782
                     /locus_tag="PW224_001527"
     CDS             22123..22782
                     /locus_tag="PW224_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL2908218.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22812..23321)
                     /locus_tag="PW224_001528"
     CDS             complement(22812..23321)
                     /locus_tag="PW224_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL2908219.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23433..24431)
                     /locus_tag="PW224_001529"
     CDS             complement(23433..24431)
                     /locus_tag="PW224_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2908220.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24632..25216
                     /locus_tag="PW224_001530"
     CDS             24632..25216
                     /locus_tag="PW224_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2908221.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25293..26486)
                     /locus_tag="PW224_001531"
     CDS             complement(25293..26486)
                     /locus_tag="PW224_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2908222.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            26550..26930
                     /locus_tag="PW224_001532"
     CDS             26550..26930
                     /locus_tag="PW224_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL2908223.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27004..27366
                     /locus_tag="PW224_001533"
     CDS             27004..27366
                     /locus_tag="PW224_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908224.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9085 a         4710 c         5812 g         8296 t
ORIGIN      
        1 ggagaaaaaa aacaaaatag aaaaaagtga ttttctaata aattattcat gtggaaatgt
       61 catttttact gtataattca tttgattatc cattttttat tcatcaaaat tactgaaaaa
      121 agaaaggaag aaataaattt gaagaaaaca aattcttcag gaaaaccgaa acgcgacttc
      181 actaaattat cgacacccca tacgtatgtc attatttttg gtgtagtcat ctttgcatgg
      241 atactgactt ttgtcgttcc agcaggaaag ttcagcacgc aagatattga atacaaagat
      301 gcaaatggcg aaacaagtac tcgtactgtt ttacgtcaag attccttccg ttatgcttac
      361 gagctggata agtcgtatgt gtttgatcaa ttagaagaac tgcaagatca tccagcagaa
      421 cgtgaaaaac tggatgttcc ggaaaaagga ttggaaaaag tcattgctga cggagaaaaa
      481 aatcttacgc aggaaaaact agatgaaatc tccttgacag atgatgtgct ttacgatcag
      541 tacggcgaaa atatctatga tacttctaaa aaattgcata aaacagccaa aatatgggga
      601 acagatgatt ttggcggctt tggtttctta aattttgttt ttgaaggact agtatctgga
      661 gataagtatg gatctgctgt agggatcgct gcattgatcc ttgttgttgg aggtgctttt
      721 ggtattatta tgcgtaccgg agcgatcgac gcaggtatct atgcgtttat cagtaaaact
      781 aagggactgg agcgtttagc tttaccgcta ttattctttg ctttttcatt cggtggcgct
      841 acattcggga tggcagaaga ggtaattcca ttctcaatgg tgatggttcc atttgtcatt
      901 gcgttaggat atgactcgat cgtagcagta accgtgactt atgtcgcatc tcaagtcgga
      961 aatgcgacct cttggatgag cccgtttagc gtagcggtgg ctcaagggat tgctggaatc
     1021 cccgtgttgt ctggtgcgac attccgattg attatgtggg tagttgttac tgcattagcc
     1081 gctggttata tgatgatata tgcagaaaaa atccgtaaaa aaccagaaag ttcgttgact
     1141 tacaaatcag atgattattt ccgttctcat atcaaaaaga catctgatga aaataaaaaa
     1201 ttcatgcttg ggcataaatt gatcttatta gagatgttag ttgtccttgt ctggatcgtt
     1261 tggggcgtga ctcaaaaagg ctattacatt ccagaaatcg cttctcaatt ctttgtgatg
     1321 ggattagttg ctggaatcat tgctgttctt ttcaaattag atggaatgaa agtcaatgac
     1381 atcgcttctt ctttccaatc tggtgcagct gatttagctg gtacagcaat cgtagtaggt
     1441 atggccaaag ggattcttct tgtcttggga ggatctgatg catctgttcc ttcagctttg
     1501 aatacgattt tgcatgggat cgggacggcg ttgaccggtg ttccagctgt cattggtgct
     1561 tgggcgatgt acatcttcca aagtttgttc aacttggttg ttacatcaaa ttcaggacaa
     1621 gcagctttga ctatgccaat catggctcca ctggcagatt tagtaggtgt atccagacaa
     1681 gtagcagttc ttgcttatca gcttggttca ggttttatgg acgcttttac acccgtatca
     1741 gccagcttga ttggtgtgct aggtgttgca cggatcgaat gggcaaaatg ggcaagattc
     1801 caaatcaaga tgcaaggatt cctgtttgtt ttaggaacta ttttcatcat gattgccatt
     1861 gcaataggtc tgcagtagta ctggagacct gtaaataaaa aagtaatcca aacagtagat
     1921 aaggactcca agaaccgtca ttttacggta aaatcaaagg agtaaagatc ctatcgtttg
     1981 gattattttt taagatccta tcatcagtta tacttaaaga aagaggaaaa atgtatgaaa
     2041 atcattcgac aaattgaagt gtttgcacca gattacttag gaaaaatgga tgtactgatt
     2101 gccggtggga agattttggc tgtagaagaa cagctagaag gcggctacga aggagtggaa
     2161 gtagaagaac tttcaggtga gggaaaagta ctaaccccgg gtttcattga ctgtcatttc
     2221 catattttag gtggaggagg agaaggcggt tatcaaaatc gaacgccaga agtcacattg
     2281 agccaattga ctacagctgg tgtaacgaca gttgtcggtt gtttgggaac agacggagaa
     2341 ggtagagata tgactgcgtt gatcagcaaa gcgaaaggtc tggaagcaga aggaatctcc
     2401 acttatgtat acgaaggttc gtaccgattg ccggtaaaaa cagtaacaga ttctatcatc
     2461 aaagactttt tgacaattga taaaattatc ggaatcggag aaatcgcagt atccgatcac
     2521 cgttcttctc agccaagttt tgaagagttc gctcgtgcag tagcagatgc tcgtgtagga
     2581 ggcatgttat ccggaaaagc tgggatcatc aatgtccact taggcggagg aaaaagaaag
     2641 ctagagctat tgacacgaat cgtagaagaa acagaaattc cgatcacaca atttttgcct
     2701 actcatgcaa atcgcacacc agaattattt gaagcttgcg tggcatttgc caaacgtggt
     2761 ggcacgattg acttcactgc tagcgaagat cctgattttt gggagaagac agatggtgaa
     2821 gttcgcttca gcaaagcact gaaacgattg atcgaggaag atgtttcttt agataatttc
     2881 acgatgacct cagatggaca aggaagcttg ccttattttg atgaaaacaa tcatttccta
     2941 gggttaggag taggaagtgc caaggcattg ctagtaggga tcaaagaagc tgtgcaaaaa
     3001 gaaagtatcc ctttggaaat cgccttacga gctataacca gtaatccagc acgtatattg
     3061 aaattggata aaaaaggaaa aatcgaaatt ggtgctgatg cagatctgtg tatcttggat
     3121 aaagaaacgc ttgatattga tacagttatc gctaaaggag aaataatggt ccaagaaaaa
     3181 gaagtcaaag tatgggggac tttcgaaaaa agtttttaat caacagctct aactatcttt
     3241 tttagaaaac gagcgttctg tttgtctgta tctgttgttg atgttaccct gaaggtagat
     3301 caataaacaa actaagagga ggaagcacaa tggcagattt aaaaggacgt tctaaagata
     3361 tgaaagataa agtagtcggt gaaggaaaag aagcatacgg caaagcaaca gacgacaaga
     3421 gcaaagaaac agaagggaaa gcacaatcac taggtgctga catcaaagaa aaagctcgaa
     3481 atttaggcga cgaagtcaaa gacaagtttt ctcgaaaaga cgaaaaaaaa taaaaaccac
     3541 ttattactaa ttgaatcaag caaataaaaa atccgaacca tatctgcgag acagatatgg
     3601 ttcggatttt ttatttgatc attatcctgt tttttaaact tcgatttttt gaaatttttt
     3661 attttttagt tcaaagactt catcacagct ttttgctact tcaatctcat gagtcacgat
     3721 gataatacag cgattttgtt catgggcgat ttcttggaaa agtttgacga tatcttgtga
     3781 agttgtttca tctagatttc cagtaggctc atccgctacg attagttgat gttcacagca
     3841 gatagcacga acgatagcga ctcgctgctg ctgacctccc gaaagtttgg tcacctgttt
     3901 ttttgctaat atttcatcaa ttcctacacg agccagtcga tctaaagcaa cttgtcgata
     3961 attctcttgc ttggtcttag tgatttccat tgctgctatg acgttgtcta gagccgataa
     4021 ataaggaagc aagttgtaag cttggaaaat aatcgagaca tcatttttac gataacttct
     4081 taacccgatt ttgttcagtg aacgatcttg atagtatacc tttccttctt ttggtgcatc
     4141 tagtccagaa ataagcgaaa gaaaagtcgt ttttccagaa ccacttgctc ctaaaatagc
     4201 gtacattttt ccagcttcga aattcagttc tacatcttta taaagcgcat cttgcggatc
     4261 ttgataccaa aatcccaatt gttctgtccg taacatcaaa ttcctccttt agtttaatag
     4321 gatttttctt gggtttaatc ttaatatccc tatagaagct aaaaagacag aaaacagact
     4381 gattccaaat gctactccgg ctaatagtcc caattgtgca ggtttgacat tgatttccaa
     4441 ttcgctgact tgatcagaaa cagcaaatgg attattcgtg gttccttgtt gaccatcgcg
     4501 ttgaccgcct tgttcttgtc tgttacctgg catttgtcca ccgccaggag cttgctggtt
     4561 ttgtgtcgtg gtttctgtct gttggtcaag tagctgctgt cccacggcat tcgcgacgac
     4621 gtttccgcta agcgaagcaa ctcctaatgc aagaatcata caaatagcca cttcggtaaa
     4681 caattggaga atgattttac ttctggattc tcctaaagat agcaagacgc ctaactcatg
     4741 ccgacgctcc cgaatggtaa tcataatgat cagagtaagt atgataatac cggctacagc
     4801 cactagtaaa acaacatttt tagaaaaact tgccacgttg tttaaaggtt ccaacattga
     4861 ctgatacatg gaatcatttg attgtaagct aaatgtatct gtgtcgatca gcttctcagc
     4921 ttctttgaga aactcatcca ttttatctgg atcagataga gtgtaaatag cagaatctat
     4981 cgtgttatcg tcagatgtcc catttagttc atttgcaaaa gtgtaagaag tgtaaagtgt
     5041 attcgacgga ttcatgaagt tgaatttcat acccatactg ccgcttgttt cgctggattc
     5101 ataaattcct ttgatcgtca tttcataagt ggtatcttca tcttcagtac tcgtgattgt
     5161 gaaagtatct ccgactgcca ggtcatttgt ttctgctaga gtagaatcaa tgactacatt
     5221 gtttgtgcct ttgtcgctgt catctattcc ttcaccatcc gtgatttttg ctgttccatc
     5281 agaaaaatta ctattttgtg cagttgctga cacaccactg atttgaaagt ctgcttgagt
     5341 catttgcgga ttcccgccca ttcctccagc aggttcaccg cctgcatcag tagtttctga
     5401 actgtcttca cttgaatcag aactagtgat ggcagttatt ccatcttttg ccatagctga
     5461 ggcggaactt tcaaaggaat aactctttac attatctaat tcagctattt tttccgcgtc
     5521 cgttaaggaa actggtgtaa gagaaaaact gcctggatct ggacgttctt cagtggtaga
     5581 accgcttgaa gaagtttgtt cctgtttttt aaatgaagct tctcgattgg cacttaaagt
     5641 cactgttgct ccaacttctt tttgtgcttg attcgctgct tcttctgcag cgcttcgaat
     5701 cgtgaggcca gctagcacaa aaataagaat ggctgtgaac acggcaaaca ataacaatga
     5761 acgtccccat tttacttttg tacttttcca tgcacgtttc ataaaattca tattttgcat
     5821 ccctcctagt agatgcattt gataatagac aacctatctt aaagctagct taaatcttat
     5881 gctgtttgca caccatcaac atgaggttct gtatatataa aaagtcacta tagttattgc
     5941 aaaaaatgtg gtaaaatgaa aaaagtatgt ttcaaaccga cgaagaagaa atgaaagagg
     6001 aatagcaaaa gaaatcctag atgctacaga gggattttta cgtgcatagt tgttctaaca
     6061 attgctgata ctagcgattt agtttatttg ttgtataata atggggaaca gctgaatact
     6121 tatataagta ttctggctat agcgtttcgc aaaagatcca ttttgttaat gaagtatatc
     6181 caaaataaaa tgcccatcca actgtcagaa cgcggatgtg ctggtaattt tcctgctgat
     6241 tctccataag ttgagaaata gtgattttgc atatgcttca ttggaatggc tcatatattt
     6301 agataaagaa gaaaatcgac atctttaaac ttggatgtca taagaagagg tgaagtttgt
     6361 gagtaccatt aaaattatcc cattaggcgg cgttcgtgaa aatggaaaaa acatgtatgt
     6421 cgcagaagtg ggcgaggata tttttatttt agattgtggg ctaaaatatc ctgaaaatga
     6481 gttgttgggg atcgacgtcg taatccctga ttttacgtat ttagaagaaa acattgatcg
     6541 tgtagctggt gtctttttaa cacatggaca tgcagatgcg atcggggcac ttccttactt
     6601 gttgtcaaaa atttctgtac cagtattcgg gacagagttg acgatcgagt tagcaaaatt
     6661 gaatgtcagt cgtaatgaag cagccaaagg atttaaggat ttccatgtgg tagatgagca
     6721 tacagaaata gactttggtg aaacagtagt gagctttttc cgtaccactc atacgatacc
     6781 agattcagtg gggatcaatc tgaaaacaaa agaaggtagt atcgtttata cgggagattt
     6841 taaatttgac caaacggcta tcccaatgta tcaaacagat tatgcacgat tggcggaaat
     6901 cggcaaagaa ggagtcttag ccttactaag tgaatcggca aatgcagaaa atcctcagcc
     6961 tgtagcttct gaagcccaaa tagcagatga agtatttgat acgatcaaat attgggaagg
     7021 gcgtatcatc gtcgcttgtg tagcaagtaa tttgcaacga gtgcaacaag tattggatgc
     7081 agcagaaaaa gcacaacgaa aagttgtctt gactggtcaa gatttcgaac gaattattcg
     7141 cacagcgatg cgtttagaaa aacttcaatt accaagtgaa gatctgcttg ttacgttgaa
     7201 agacatgaaa aactatgatc cagaagaact gatcatttta gaaactggcc gaatgggtga
     7261 acccatcaag tctctgcaaa aaatggcaaa cggtacacac cgtaatgtca agattgaaga
     7321 aggagatttg gtttatatca ctactacgcc aagcatcgcg atggaaacca tcgtggcgaa
     7381 aacggaagat atcatttatc gagctggtgg aacagtgaag ttgatctctg agaatatgcg
     7441 cgtttctggc catgccaatc ctaatgactt gcaattaatg atcaatttga tgaaaccaac
     7501 atattttgtg ccagttcaag gagagtatcg tgaattagct gctcatgcag atttggctca
     7561 tgcagtaggg atgccataca aaaatatcta tattactgga cgcggagacg tgctggaata
     7621 taagaacaag cgtatgagtg ttgcaggaac tacaagtgca gaaaatgtaa tgattgatgg
     7681 attaggcata ggtgatatcg gaaacattgt tttacgtgat cgaaaaatct tgtcagaaga
     7741 cgggattttc gtagcggttg tcactatcag tcggcgagaa aagaaaatca tttcaaaacc
     7801 tcaaattact tcacggggat tcgtttacgt aaaagctagc cgtgatctga tgaaagaaag
     7861 cagcaatatg attgaaacaa tcgttgagaa acatttagaa agcaatgatt ttgagtggag
     7921 caaattaaag caagacatcc gagatcaatt aggccgttac ttatttgaac aaacaaaacg
     7981 ccgcccagtg atcttgccag tcattatgga agctactcaa cgtcgaaata aaaaataaac
     8041 aaaaagagta agatgaacct ctctatagag tgctcgtctt gctctttttt tgcgaaaaac
     8101 aaataatttt tgaatacagc atccctcgct ttataataaa cgatagaata aagggagtga
     8161 atcaatgatg agtcaaacaa aagtcgaacg ttatttagaa gaacaaaaga tcagctatca
     8221 accacttgat tttagtcatg tggtagaaga atctttcaca gaagaactac aaaaaagagg
     8281 gattgatcca gctttgatat gtaaaactct cgtagtgaaa ggaaacaaaa ctggggtgat
     8341 catcgcattg attcctttga acgatcactt agattacaaa aaaacgagac aagtgacgaa
     8401 agatcgtaaa gttggttttc ctggaatgga ttttgtatta atgcatacag gatatcccca
     8461 cggagcaaac acccctattg gaataaaact cgcacatcct gattattgtt ttttagcaga
     8521 cagttcttta aaagaaaaag aagaagtaat tgtgtcaagc ggtgaaatcg ggagaagcct
     8581 tcggataaaa gtagccgatt tagaaaagat cgtccatcct atttacgctg atttaattag
     8641 ttaagggacg agaagagaag ctaagcagag cagagacctc aaacttgaga ttatatctgc
     8701 ttggtacttg gtgcatcctg tattatctgt tacaattgct ttgtacatac tgtactatca
     8761 atccaatgaa aatatattta gcagctatat tttttcattg atgtgcaagt gatgaaatcg
     8821 tttttaataa aaaagataca gagaaaacgc gaggtgtaaa atatgaaaat tacgtatcat
     8881 ggacattcct gtttaacaat tgaaatggaa aatggccaac gcgtgatgat tgacccgttt
     8941 attacaggaa atccattgac agacttaact gcagaagaag taaagacaga ttggttgctg
     9001 attactcatg gtcatagtga ccacatagga gatatgattc ctattgcaaa agaaaatcaa
     9061 gccacaatta tcagtattgt tgagattgct gattatgcga acagcagagg agtagattca
     9121 ttcggtatga atatcggtgg aaaacatgcg tttccattcg gcgtggtaaa attcgtacat
     9181 gcacagcata gttcaagcta tgaagtagac ggtatcgttc agtatatggg cgagcctagc
     9241 ggaattatta ttcaagcaga aggaaaaacg atttatcatg caggggatac tgcttatttc
     9301 agtgatctag gattgcttgc agaagaattc gatattgatg tcgctttctt gccgattggc
     9361 gataactata cgatggggcc agaagatgca gcaaaggctg cgcaaacaat tcgtgccaag
     9421 aaagtagttc caatccatta caatactttc cctgtcattg aacaagatcc cgaatctttt
     9481 gtagagttac tggaagatag tgaaggaaaa ataatgagcg tctcggaaac gctggaagtg
     9541 taggcagaaa atggcgacaa aacatgattt gattttagaa tatattgaaa gtttacctgt
     9601 tggcaatcga atttctgtaa gaagcatcgc aaaagaattg aatgtcagtg aaggaacagc
     9661 gtaccgtgca atcaaagatg cagaaaatgt cggactagtt tcaacaattc aacgagtagg
     9721 gaccatccgt atcgaacgca aattaaaaaa acatattgaa cgtttgacat ttggcgaggt
     9781 cgtcaggatc attgaagggg acgttttagg cggggcggca ggattagaca aggtcttgaa
     9841 taaattcgtc atcggcgcga tgactaaaca tgccatggaa cgctatatta cgccaggatc
     9901 actaatgatc gtcggcaacc gggcggaagt ccaaaaatta gcattggaag atggcgccgc
     9961 agtattgatc acaggaggat tcaatacgac tgctgagatt gcaaagctag ctgatgatct
    10021 tgaaatgccg atcttacgca cctcttatga tacatttaca gtcgcaacca tgatcaaccg
    10081 agcgttaagc gaccaattga tcaaaaaaga tatcatgctt gtcagtgata tctatatgcc
    10141 agctgaaaag actcattatt tgcagcagac agatacgatt tctgattatc agcgtttgtc
    10201 agaagaaacg cagcattcgc gttttccagt cgtcaaccgc catcatcgtt tgatggggat
    10261 tgttacggca aaagatgttt tagggaaatc gccaaaccag atcatcgatc gggtgatgac
    10321 gaaagaacca atcagtgtaa agaaaacaat gagtattgcc tctgttagcc atcaaatgat
    10381 ctgggatggg ctagaagtca tgccggtcgt ttccgatgac ttgacattgg aaggcttgat
    10441 tacccggcaa gatgtaatga aagcgatgca gcttgtccag cgacaaccgc agattgcaga
    10501 tacgatttcg gatcagatat ctggaaatat ccatacgatt gatactgacc gtgaaggaaa
    10561 tcgtttggaa aaccctaagt tcaaattcgt agtagcacca caaatggtca atggcgttgg
    10621 aaccatttct tttggcgtac ttagcgaaat catttctgat gtcgcacaaa aaacaatggt
    10681 gatgaaccaa aaacgaaata tcttgattga acaagtgaac ctacattatt tgcggctgat
    10741 ccaattggaa agcgaattag acattcgtcc acgtgtattg gaaatcggca gacgttccgc
    10801 taaactggat atcgaagtgt ttattgaaaa caccatcgtt tccaaagcaa tcgttgtttg
    10861 tcaagtaatg gaacgttctt agaaagcagg agtgaaatga cagtacagca agaaatagta
    10921 gatacaataa aaaaatacga taaaatcatt atccacaggc atttgcgccc tgatcctgat
    10981 gcattaggat cacaagtagg attagccgaa ctattacggg aaagtttccc agaaaaagaa
    11041 atacgtcaag taggtgaaac gattgaaggg cttcgctttt tagcagaaat gcaagaagtt
    11101 gaagatgtct tctaccaagg agcgctagtc atcgtgactg atacagctaa ttcacccaga
    11161 atcagtgatc aaagatatca gttggcagaa aaactgatca aaatcgacca tcatcccaat
    11221 gatgaccctt atggagatct tgtgtgggta aatacaaaag caagtagctg tagtgagatg
    11281 attgcagaat ttgctttgat gtttcctgaa tctttaaaga tgaacgttaa ggctgcacgg
    11341 ttactttatg caggaatcgt cggagataca gggcgctttt tgtatccctc cacgacgtcc
    11401 cgtacattag agattgcttc gatattgaga aattatccat ttgatgcaag tgctttgaac
    11461 agagagattg aacaaatgcc gatgaaagtc gctaaacttt ccggttatct ttatcaaaat
    11521 atccaggtgg atgaaaatgg tgcaggaaga ataatccttc cgcaagaaat tcttgataaa
    11581 tatggaatcg atgattcaga gacagcttcg atcgtttcac ttcctggagt gattgacgaa
    11641 gtgctggctt gggggatttt tgttcagcag ccttcagggt attaccgcgt acgtttacgt
    11701 tctaaggggc cgatcatcaa tgaattggct aaacgccatc atggaggagg ccatccatta
    11761 gcaagtggtg cgaatgcaaa agatttagca gaagtagagg aaatctataa agagatccaa
    11821 gatatatgcc aatcgtataa gagcagaaaa acagaatgat acttcgttcg aaaacgtgcc
    11881 ttttgtagta aaaatcacaa atgtatcttt tcaagatctt ctttttccgt tacaatagtg
    11941 ctcactaaag attttaaagg agaaagaaga tgacattacc aaattgccca gaatgtggct
    12001 cagagtacac atatgaagat cgaggcttgt tgatttgccc agaatgcggg aacgaatgga
    12061 acaaagaaga acaagaaact gtcatagaag acggattagt ggtattagat gcaaatggaa
    12121 accgtctgtt agagggcgat agcgtgacag tgattaaaga cttgaaagta aaaggtgcgt
    12181 caggtgccat taaacaagga acaaaagtca agaacatccg tttagtagaa ggcgatcata
    12241 atatcgattg caaggtagaa ggatttggac cgatgaaact gaaatcggaa tttgtgaaaa
    12301 agaattaaag aatattcaag gtcttttcac attcttttcc tactactaat gataaaataa
    12361 agtaaacgaa aaggagtgaa acgaaaatgt atggttatgg aatgtatggt tttttagatc
    12421 cgacatttct tttggtcatt cttggattag ttatttccgg aattgcgtca gcgtacgtca
    12481 atagtacttt ccgaaaatac gatcaagtga gaagcaaaaa caatgtgact ggtacacaag
    12541 cagcacaata tatattacaa agtcaacaga taaataatgt cggtgttcaa caaattgccg
    12601 gagatctcac agataactac aattcaggta ataagatgtt gagtttgtct caagcaactg
    12661 cacagtctac ttcagttgct gcgataggtg tagcagctca tgaatgcgga catgcggtac
    12721 aagatgcttc gggttatgtc ccattgaaga ttcgtgcagc acttgttcca gcagctaact
    12781 ttggttcgat gatttcgttc cccttgatta tggtaggtgt attgcttagc tggaactcta
    12841 cgttaatcaa tatcgggatt ttcgcttttt ctttagcatt acttttccaa ttggtgacat
    12901 taccagtcga atttaacgcg tctcggcgag caatccaaat tttgagtgaa ggcggactac
    12961 tgacggaaga agaagtaccg atggcaagac acgtattatt tgcagcagca ctgacttatg
    13021 ttgcagcagc gctttcgaca ttcctgcagc tgttgcgctt gatcttattg ttcggcggca
    13081 accgaagaga ttaaaataaa gaagttgtga aatcagcgct ttgatctgag ctaataccga
    13141 gagttagctg attgaatacc atttatttgg aataagagga tgtgacaaga attttgtcac
    13201 atcctctttg ttttgttctc ataacctttt ttcatataca gttcatgaac cgcttttatt
    13261 aacttgccaa ataacactgt attgtgcgtt gtatccagat agaaagtctc cgtttcatct
    13321 gaaattttct catgctgatc tgtaatgatc aaatcagcat gttctgaata tgcggtaatt
    13381 ttaacagcat ctttgcgaaa taactgctgc aaatggcttt gaagatgata atttcctgta
    13441 aactgtttgg ttatctgcaa atggatgagt aactgctctt tcgtttctgt ttgtagtaag
    13501 tgttggatca attgactaag gtattgactg atttggtttc tttccccatt ttttgaaaaa
    13561 attgtatcga ttttttctct taatttcttt tcctgtataa aagaactaga atgattggac
    13621 gtggcttctg gaaagcaaag tgtagaaaaa ctactgctgg attcacccaa cagttcgaaa
    13681 aaaacaactg ccaacacaag aaaataaaaa taaattggta ttttattttc taaatggttg
    13741 agaggaaact gttgagttaa gtaagtcatc aattgaccga cttgtcgaca aataaggttt
    13801 ttgcttgtgg aaaataaaaa accaatctcc tgtttttgtc gaaaagagat ttgatttgga
    13861 aaaaaacaat agaaaaaata attaaagtag gctctctctg tttctgtctc ttcacaaggg
    13921 cgactcactg aaaaagaagt aaagtattct gaataatcat gtgtatgctg caataagtcc
    13981 atcaattcag tgatttcaga agaagttttt ggaaaatatg cctgttgttt gtttcgtaat
    14041 atgaataaag ttaacagcaa gtagatattt ttttcttgtt ttgaaatttt ttctatttgc
    14101 tcatctgtac tgttcatttt tagtgcattt aattcaagtg aggaaaatgg ccaatcgtta
    14161 cctccaaatg tatctgaaag aaaaaagtaa agatagagac ggatagataa ctcatttcct
    14221 tcaagagtac agcaattttt tacagatagg atccgtaaat ggaattttat taaggaatga
    14281 ttgagctgtt ttgtcagttt ttcgagatag gagggcgata tataaagttg ttcaagtaaa
    14341 tatttttttg gtacagcagt ttgtggataa ttgattagta acatcaaata gttaaaacga
    14401 ttggattctt ttaggtagca taattttaac ttagaaaaaa cagctaactt tttagtttga
    14461 tcggtttctt tggtttggat ctctccttta tgattagaat aaataaattc atcaatcaat
    14521 aaaaattgct tgagtgatac atttattttt cggatcagtt ctttgattgt ccgttttgga
    14581 atatgtaagt cattggataa ttcttgtaaa gaacaattat ttttttgaat gaccatttgt
    14641 agaacaagta actctttttt tcttgcttca attagcataa atacctcctt tttattctgt
    14701 ttttgtatat agttagacaa aaactgtgag tggaaagtca tttcagtctg tgttattctc
    14761 acagagtgtc gccttctttt ttatataacg ttatttaatt tttttgttac cttgtttatt
    14821 ctattgttta tttcctaggg tattttgtca agatattctt gtttttaagg cggaaaaatt
    14881 gatttttaat ggatataact aaatatgttt tgataattat caataatatt tttagaatta
    14941 tttatgttat ttatttatgg gtagatgaaa agagacggag gtaagaatga tggggagagt
    15001 aacaaattat agtaaagaat atttgctgtt caaaagtatg gtatacgtgg aagaatatgg
    15061 aatgaagtca attactgcaa gagagttagc agagttctgc ggttgttcta catacccgat
    15121 ttatacacat ttcaaatcaa tcagcgggtt aaaagagaag atattagaag aaatcactgt
    15181 ttattttgaa aactacttag ctgaatgcag ttccgatgat gttttaagca ttgtctactt
    15241 attgaaagat tttttttcta tccatgaaag tattcgtgaa atcattgcaa aatctgattt
    15301 agacatggat tcgctattta agcggccatt tttctcatat gtcaaaggtc acacgaagat
    15361 caaagaagaa aagtcgcttg aattggtatg gcttaatgca ttgggaagtt tgcgctcaga
    15421 tgattcttct caagattttc ttgaaatgat gaacgctgtt tgggataaaa acgaggtgac
    15481 ggatgaatac ttggaaaata tcttaaagga acagtcacct taggcagcaa catgaaaaag
    15541 tagatcagcc acctttattt ttcgtaaaaa agattgtaaa atgtctaacg aaacattgga
    15601 atgaaggagg aagcgacaac gttaccacag caatttatca aaaaatatcg tcttctttta
    15661 ggcgaagaag catccgattt tttttctgcg cttgaacaag gaagcgtgaa aaaaggattt
    15721 cggtggaatc cactaaaacc ggcaggattg gacatggtcc aaacttacca tagcgaagaa
    15781 ttacaacccg ctccctacag caatgaggga tttcttggca ctgtcaatgg aaagtctttc
    15841 ctccatcaag ctgggtatga atatagccaa gaaccaagtg caatgatcgt tggaacagct
    15901 gctgcagcaa aacccggtga aaaagttttg gatttgtgtg cggctcccgg tggtaaatca
    15961 acccaattag ctgctcaaat gaaggggaaa gggttattag tgacgaatga gatctttccg
    16021 aaacgtgcga agattttatc agaaaatata gaacgatggg gagtttcgaa cgcaatagtg
    16081 acaaatcatg cacccgcaga acttgtccct catttttcgg gcttttttga tcggatcgtg
    16141 gttgatgctc cttgttcagg agaaggaatg ttccgaaaag atccaaatgc aataaaagag
    16201 tggacagaag aatctccgct atactgtcaa aaacgtcagc aagagatttt gtcttcagca
    16261 ataaaaatgc taaaaaataa aggacaactg atttattcga cttgtacgtt tgcaccagaa
    16321 gaaaatgaag agatcattag ctggcttgta gaaaattatc cagtaacgat tgaggagatt
    16381 cctttgactc aatccgttag ttcaggacgt tctgaatggg gaagcgtagc tggtttggaa
    16441 aaaacaatac gtatatggcc tcataaagat cagggtgaag gacattttgt tgcaaagctg
    16501 acttttcatg gacaaaatca gatgcataaa gaaaagaaga cacgaaaaaa aagtaaagtg
    16561 caaatgacaa aagagcagga aaaactctgg acggagtttt caaatgattt tcattatgaa
    16621 gctactggaa gacttttagt gttcaacgac catctttggg aagttcctga gttagcccct
    16681 tctctcgatg gtttaaaagt cgtacgtaca ggtttgcatt taggagattt caaaaagaat
    16741 cgatttgaac ctagctatgc attagcgctt gctacaaaaa agatagagaa tataccttgc
    16801 ttgccgatca ctcaaaagga atggcaatct tacacagcag gagaaacatt tcaacgtgat
    16861 gggaatcaag gatgggtttt gctagtatta gataagatac ctgtcggatt tgggaaacaa
    16921 gtaaaaggaa cagtaaagaa ctttttccct aaaggattga gatttcactg agtggattag
    16981 ttcgtttatt tttttgtcta tgctatgatg gagatagata aaaaggagct gatacttatg
    17041 aaaacatcaa caaaagtaac aatcggatta agcgtagcag ccgctgccag tgtagcgaca
    17101 gcagtcgttg tatcgggaaa agtaatggaa aagatccatc atatgactac tcggacgaaa
    17161 gtaaaaaaat tcgtctatga caaatttgat ggaaatgaaa aattactaga aatcgtagat
    17221 ggtctttcag atagtgactt ggattctttg atgggaatgt tagcaaaaat caaatcagga
    17281 aaaaagaaaa tctctgttta tggtgattca atcaaagatt caaccgaaga tgtaaaagac
    17341 cggctaatgc attttgttga aaaaatgatg taagagaaac tagcttttga acactattca
    17401 ttcgaaataa ggggttgtga caaaattatt gtcacaaccc ctttaatcta tctttaatta
    17461 tgttcatgaa gcaataaaag atagcaaaac agtaaatcgt tggctttttt tagattgaaa
    17521 ccagactgtt cttgaaattt ttcgattcga tattttagag tattacgatg aagaaacagt
    17581 gtttttgcag cagagctgac attgcctaat tcattccata attcttgaat gatcacttcc
    17641 atttcatttt gtttgatcag ttctatgtaa cttttgatca acttactatc ttttacagta
    17701 tcttttgtat agtaatgtaa agccacatct tgcatagaaa aagtacggct gtgagtataa
    17761 agattgtttt gctctgttaa aaaaattttt ctttcttcag caaacaaagg gacgaggtcg
    17821 gtgccaggtg aatggaaaga accaacgaat acagacgttg tcgtatcaaa atcagcatct
    17881 aaagatagaa aaataccgtt gatttcagta gcatcaagat aatcacggtt tatttgttcg
    17941 actaagagcg catctgtgtc tgtataaaaa aagaaatcag cagaatagcg aaataattcc
    18001 aaaatagtat cttgccattc tttttttaag aattctcctt ttgattcgac gtgaagctgg
    18061 atcaaacgaa agcttttttc ttgaacacaa gaagcatctt taaaaagata gtgataccat
    18121 gggtgttttt tttgatcgtt gattacaggg aataacgctt tcaaaaggct ggtttcttgt
    18181 tgagataatg aatcttgttt gatccagata aaagcatcat caattgcaaa agaaaggata
    18241 tccgcttctg atgctggaga gttttttttc tgtgcttgtg ggtaaagagc aagtaattta
    18301 tccattttca tcttgttcac ctcttgtatt tattttacca tagaaaaaaa agttagttac
    18361 agccttttta tcatatgcta aagtaaaaca agaaaaaaca aaggcaggga agggtaattg
    18421 gtaaatacaa atcttgatgc agtagaaaaa gaaaaatgga tgcgccttgc attggcagaa
    18481 gcaaagaaag cagaaatgct tcatgaagtt ccgattggag cagtggttgt attggatgga
    18541 aaagtgattg gaagaggata taaccttaga gaaaccacgc aagatgctac tacacatgca
    18601 gaaatgcttg caatcaagga agcctgtgaa aaagtgggaa gctggcgctt ggaagatgct
    18661 gcactttttg ttaccttaga gccttgtcct atgtgcagcg gcgcgatgat tttgtctcgt
    18721 gtagcagaag tttatttcgg cgcgtatgat ccaaaaggcg gaacagccgg taccttgatg
    18781 aatcttttgg aggacgagcg gtttaaccac caagcatatg tagaaggtgg cattttagaa
    18841 gacgaatgtg ggatgatttt gactgacttt ttccgaaagc tccgccagcg aaaaaagaaa
    18901 ttaaaaaaag actggtcaac gaatgaaaaa taaggtatac tattctttgc cgaaaggcta
    18961 ggacaatggt gggcttgtga agcgtgtcag gtctggaagg aagcagcact aagcaggttc
    19021 cgccatgtgt ctgattataa tgaaacgaca acagactttc gggtctgttt tttttatttt
    19081 tatagtactg taaaaatgaa tgttcctgat tttttagcca gactgattta tcgaatattg
    19141 tacttataca atttgcttca aggcaggaaa aactctgaaa tagagcttgt ttcaatggaa
    19201 gaaaaagctg cctatggtat gataaagata aagagaggcg agagggtaca tggaaaaaat
    19261 agtgatttta catacgaatg atctgcattc tcatttagaa aattggccaa ggatcagaag
    19321 gtttttggat caacgtaaac gagaaaacga aaagaaagaa aaaacgacaa cgattgcagt
    19381 agatctaggt gattttgtgg atcgttggca tccattgtca gaagcaacga atggacagga
    19441 aaatatcttg ctgatgaatg aagttggtta tgatgcagcg acgataggaa acaatgaagg
    19501 cgtaggaaat tccaaagaag agttgaatca tttatacgat catgctgagt ttgatatcat
    19561 cctggataac ttattcgata aaaatacgct ccagccaccg gcatggacga aaccatataa
    19621 aatcattaca acgaaacacc agacgaaaat cggactaatt gcatttactg ctccatttcc
    19681 gcttacttat aatccgaatg gctgggacat acggaatcct tatgatattt tgccagaatt
    19741 agtcgaagat ttacgttcac aagtggatgt tttagttttg atgagtcatc tcgggattca
    19801 ggatgatttc caaattgcaa aagaaatacc agcaattgat gttattttag gatcgcacac
    19861 ccatcattta ttcagagaag gtcacgtaat caatgatgtc caaatagcag ccgctggaaa
    19921 gtacgggcag tatgtcggtg aagtccattt aaccatcgat gaagagaaaa aaatccttaa
    19981 gcattcagcc aaagccatcc ctaccgaaac aatgacagct tttacagaag atgaacaaga
    20041 aataaatagt tatctagaac gtgggcatga attattaaaa gaaaaagaag tagcagattt
    20101 gccattttct ctgagtctag atatcttcaa tgagcattcg tttatccaag tagcctttga
    20161 ggcggtgaaa gaacgaggaa agacggaagc agcaatatta aacagtggtc tgtttttaac
    20221 tgaaattcca aaagggcggg tcaatcagga tcagcttcac actgcgttgc cgcatccgat
    20281 gcacttgtta aatgtcacat tgggtggaaa tgatctgatc cgcttagttt tagaaataga
    20341 aaaaaatcgt aactttttac gtaactatcc tatgaaggga atgggatttc gcggtaagat
    20401 ttttgggcag atggtctata atggaatcac atatgattct attaaccatc aagtcttttg
    20461 gaagaacaag ccaatcgatc tgaataaaaa ttatactttt actaccgtag atcatttgat
    20521 gtttgttcca ttttttccaa cgattgagat tgctggaaaa aatgaatttt tgttccctga
    20581 atttatccga agtgtggtag gagattactt gaaagctcac tacccaatca aataaaaaca
    20641 aggtataata aaaaagatag gtggtgaacg catgtctgca aaacaaaatg aacagcagaa
    20701 cgttgagcat aaaacagaaa ttgctaagaa aaaatcatca gctgaacagg aaacaacagt
    20761 caccgaagaa ctgacagctg ctttggaaga aatcgttgag gagcctaaag cagaaaaagt
    20821 caaaggcgag ctagtgacac tgttagatga cagtaatttt ttgataggtg aaagacatta
    20881 tcggcttgtg tccgattatc gtgaaggatt taatgcagaa aagcttggag aacgttacag
    20941 tgatgtacta gcccgatatg attatatcgt aggagattgg ggttatgaac aactacgcct
    21001 aaaaggattt ttccaagcgg ataatcgacg ggcgcatcca gatcagcgaa tcgattcctt
    21061 ggaagactat ctttacgaat attgcaattt tggttgtgcg tattttgtca tcgagagaat
    21121 cggcggtaaa aaagaaaaga caactcagcg ccgaaaaaag aaaaaaaacc ataatcgtgc
    21181 gtatatcgat gagaaaaaag gaccagtcgc atctaatcgc aaaaaaccgg ttattaaaaa
    21241 tcgaaaaaca gagtcagcaa aaacctctga aaaagtaaaa aaacaagaac aacctaaaca
    21301 agtagaaaaa acaaaaacag catttacgat cagaaaaagg gaagagtagt ggatgaatgg
    21361 actataaagg atatttaatt gatttagacg gaacgattta tcgtgggaca caacccatac
    21421 cggccggtaa acgatttgtg gaagaattgc aaaaaagaaa attacctttc ttgtttgtta
    21481 ccaataacac gactaagtca ccagaaacag tagctaatcg tctagcagat gagtttgaca
    21541 ttcatgtagc tccagagaca gtctacacag ctactttggc aacaatcgat tttatgaaag
    21601 cagatggaaa agggaaaaaa gtctatgtta tcggagaagc aggattgatt gacttgattt
    21661 tagctgccgg atttacatgg gaagaagagg ctcctgacta tgtagtggta ggcttagata
    21721 attatctgac ctatgaaaaa gtagttaaag caacacttgc tattcaaaaa ggtgctactt
    21781 tcattggaac gaacccagat aagaatatac ctactgaacg cggccttctt cctggagcag
    21841 ggtcagtcat ctcttttgta gagacagcaa cccaaacgcc gccgatctac atcggaaaac
    21901 cagaagccat cattatggat aaagccgttg aagtattagg gctgcaaaaa gaagaagtaa
    21961 tcatggttgg agataactat gaaacagata tccaagctgg gatacgcaat aatatagata
    22021 ctttgctcgt tttatcaggt tttacaaaag aagaagatgt acctgggttg cctatcccag
    22081 ctacctatgt gaaacaatca ctggatgaat ggagcttcta atatgaaaaa ttcgagatgg
    22141 cagtggatgg aatatgctgg attgttcagc ctatttctga cactgatcag tctggcagtt
    22201 ggtgtgacga tcaactttcg accactatac gtatttgata tcggacattt gcaaattttg
    22261 gattatactt ctttagatca agagacgtta ctgaaaaatt ttgatcacct aatgaattat
    22321 ctcaataatc ccttcaaaac catcctcagc ttgccagatt tcccagtttc tgctagcgga
    22381 gcccatcact tttatgaagt gaaaattttg tttttagtgg attatgctgt gtttttcatc
    22441 actttgatcc cgagtatttt gtttatcaaa tatttgcaaa agaatgatcg gctttggcgg
    22501 ttgattcgtc cgtttcaaat cggcatgtta ctgccagttg ttttcggttt tttcatgatg
    22561 ataggatttg atcgcttttt tatccttttt catgaaacgt ttttcaacaa cgatgactgg
    22621 ttattcgatc ccgttactga tccaatcatc aatgtacttc cagaagaatt tttcatgcat
    22681 agctttatct tattttttgt attattagaa ttattttttg ctgtttttct tttcttagga
    22741 aaaaatagct tgaaacaaac aaaaaagaaa gaactcgtct aaatagacgt tttctttctt
    22801 tttttgttga attattgagt aaggcgactt cttttttctg gttctttgtg tgctaatcga
    22861 ctggcagcag cagcggcgat cgctccgacg atatcatcta agaaggtatg gacatgaacg
    22921 ccatcgtggc tgtttagttc ttttaaaata ccaggtttca ccttgtcgat ataaccataa
    22981 ttcgtaaagc cgatcgtacc gtaaacattc acgattgaca aggctaggat ctcatcgata
    23041 ccgtaaaggc cttcgtcatc ttcgataata tgttgcaacg gactcatcaa ttttttcttt
    23101 tctgctaata tatctaattg gattccagtg ataatggcgt tatgtacttc acgctttgtt
    23161 agcacgctgt tgacactttc agtacaatcc gccaaagtca aatcagaaat atagtctttt
    23221 tgtaaaaaca tgacaagttg tgcgatatct tctacagtta cgccacgttc cttcaatagt
    23281 tctcttgcct tttcttctaa agtctcagtt ttgacgacca tattattgct ccccctttat
    23341 tttttctttc tctatttcat gttaacggaa catgaaaaaa atgcaagaaa ctgctttcgg
    23401 gtttaacttg aaatgcaaag agatataaaa aatcaactag gacgaagatt tttatcgtat
    23461 aagcttgtgc tgtgaccagg ctgagttcgt tcctttggat taatatagtg taaagcgttg
    23521 ttcactgcag taggtgcttc accgagccca gtagcaatca gtttcacttt tccttcatag
    23581 gtacagatgt caccagctgc ataaacgcct ggaatagaag tagacatatc tgattttaca
    23641 gtaatggcat ttctggtact atcaagtccc caagaagaaa gatgttctaa attagaagag
    23701 aatccataat tgacgatcag agaatcaatc atcaaatcaa tcgtctcgtc acttttcact
    23761 ttttttaacc ggatatctgt cagttcccca ttattgcctg ataatccatc gatgagatag
    23821 ggagtaagca agttgacaga tgaagatttc aagcgtgata cactatgttc gtgcgcacga
    23881 aattccgggc gacgatgaat caagtaaact tcgcttgcaa taggctctaa catcaacgtc
    23941 caatcgatag cagaatcacc acctccagca atagcgactt tttttccagc atacttcatc
    24001 aaatcattca cgaaataatc caatccgtgg ttttcaaatg attcagcatt atctaaattc
    24061 aattttcttg gctggaatga accatttcct aaagctaaaa tgactgcttt ggaataatgg
    24121 atgcctttat ttgtagtaat ctcgataact tcgtcttcac gagtaagcga gagaacttct
    24181 tcttttaaat ggaatgtatg gttaaaagta gttaattgtt tttctaattg atcgattaat
    24241 tcacttgctt ttattgctgg atacccagga atatcgtaaa tatatttttc gggatacaaa
    24301 gttgctaatt gaccaccaag ttgaggtaga ctgtcaatga tttttgtttt agcttggcgc
    24361 attcctgcgt aaaaagcggc aaacatccct acaggacctg caccgatgat tgtaatgtca
    24421 tagatttcca taaaaattcc tcccttttca cactatatca gaatttaatt gagaatgctt
    24481 ctcaaaaatg tttgaattcg aaatcgaaac ggtaagttat agaatgtatc tagtgatgaa
    24541 aaataaaaga aaacaaatct agtattttta cgagaggtta ggtattatgt tattattatt
    24601 atgaagtatg caaatattag gaggaaacaa tatggcattc ccacaactag atttaaaaag
    24661 tgaaaaagga ccaaaagctg ttatcaaaac aaaccgtggt gacattactg ttcaattatt
    24721 ccctgaacta gcaccgaaga cggttcaaaa tttcattgaa ctttccaaaa aaggttatta
    24781 tgatggcgtg atttttcacc gcgttattcc tgatttcatg atccaaggcg gcgatccaac
    24841 tggaacagga atgggtggcg aaagtattta tggcgaaagt tttgaagatg aattcagtcg
    24901 tgaactgttc aaccttcgcg gagctttatc tatggcaaat tcaggtccaa acacaaacgg
    24961 aagccaattc tttattgtaa ataacacaaa tgtgccagct aatatgcttg gacaactaga
    25021 aggtgctgga ttcccatcag aaatcattga agcgtataaa ggtggcggaa ctccttggct
    25081 agatttccga catacagtct ttggacatgt actagaaggg atggacacag tagatgaaat
    25141 cgcaaatgta caacgtggtc cacaagaccg acctgtacat gatgtagtga ttgaaacaat
    25201 tgaaatttca gaatagtttg gtatggtacg gagtaattat gaaaaaggac gaaacctagt
    25261 aaggtctcgt ccttttattt tgaataaatg atttaatcga ttgtctctaa agcatacttg
    25321 attcgattaa gtccttccag taaagtttct tctggacaag caatattcaa gcgcatatgc
    25381 ccatgaccgg aaggaccaaa cgtgatgcca ggatttaata cgacttttcc tttccgaaca
    25441 agagtgttct ccaattcttg gtcgtctgaa atataggcgg taaaatctaa ccacattaag
    25501 taagttcctt cagggttcat tattctcact ttcggaagat actgttcaaa aaactgatta
    25561 actgtttgaa catttttttc tagataagga atcagttggc ttaaccattc tccacccgtc
    25621 tcataagccg cttgggttcc aatcagtcca aaagtgttga tttcgtgctg ttggttcatc
    25681 actaaatgtt tttcaaaatc agctttcatc tctggatttt tgataaatac cattgaattt
    25741 ttgattgcag ctagattgaa agtcttggtt gcggaagtaa aagcaactag tagatcatct
    25801 aaattaggat caatcgttag catggaagta aatgtatgat cgaataaagt taaatcttga
    25861 tggatctcgt cactaaacag aatcacttga tgttttaaac acaattcgct taactgtttc
    25921 aattcttctt tgctccaaac acgccctcca ggattgtgag gattgcataa aatcattgct
    25981 tttacattgt tttcttcgat ttttttctcc atgtcagaca agtccatgat gaaatgatta
    26041 tttttttcta gcaaagagct tcgaacgatg tgacgttgat ttgtttctat aatagaggaa
    26101 aatggaggat aaactgggtc atgaatcaaa atagaatcgc ctggttttgt aaaagtttgt
    26161 actgctgcag ctaaactggt taacacacca cttgtaaaaa cgatattttc tttcgtcaac
    26221 tccacagcat ggtgttggcg ttcccaatga atgatagcct gatatagttt atcagacaga
    26281 gtggcataac caaagattcc atgtttgatg tattcactaa acgctttagc taccccaccg
    26341 ggagacaaga agtccatatc tgctacccac aaaggaagta agtcatccat ttgatatgtc
    26401 tcagcaattg cgtcccattt tacactttct gtatcttttc ttgaaatctg tttatcaaat
    26461 tcgatcatct taagacctcc agtcattttc gtcgcttctt tttttgagct atttacagta
    26521 taatgataaa tgcgaagaga gggaagtaga tgacttataa aattggacaa ataattgaag
    26581 gaaaagtgac agggatacag ccttatggcg cattcgtttc tcttgataac gagacgcaag
    26641 gacttattca tgtttcagaa gtacaatccg gttatacgaa aaatatccat tcattgttaa
    26701 aagtcggaca gccagtaaca gttcagatca tcgacattga tgaatattcc aaaaaaatca
    26761 gtttatcttt aagaacgtta gaaaaaacaa atccagctgt tccatatcgc agaaaaaggt
    26821 atttcacgaa taaaaatcgt aagatcggat ttaccactat tgcggagcag ttacctattt
    26881 ggatagaaga agcattatct gaactagaac ggaaagaaaa taagaaataa aaagtgtatt
    26941 ttttctgaaa atgtgctaga atttttatgt aagccataga acaagttgat cgaggtggaa
    27001 attttggcaa gcaaaagagt tgctggtaca attatgttgc atttggagga tggtactaaa
    27061 aaatttttgg tccattcaat tgatgacaag ttggaatttg cgttagcgga actttctgaa
    27121 ggaaagactg gtttagcgaa tatacttaat ttcttaaaag aaatagttca tatcgatgtt
    27181 agcaagatta gtttgatgga attgacaaat acacatatta aagaggaaaa tgttccgttg
    27241 tttgtctttg aaactgaaca aaagaatcaa agagaagaat taggcgaagg atatatttgg
    27301 gaagaaccag gtcaaatgcg ctctgttctt ggaacttacg aagtagaggg agtgcctttc
    27361 ttctaaaata cagattaagc gaacaaagaa gtttgaattt ttttcaaact tcttttttta
    27421 ttgtttggtc caaaaaatac aatgaaatgt tctgctaaag atagtttttt agtgcatttt
    27481 attttcaaaa tatgttgaaa acggttgtat aacgaataat aataattgat cgaaaaatca
    27541 tttcacaaag taatgcttaa tttatagttg accaaggaag aaatacatga taaatttaac
    27601 agcgttgatt ctgaagtgga tgttatatca catttctaaa aaatttaaaa aatagttatt
    27661 gacatgttac taacgacatg ataagataac tgagtcgtta aaaaagaacc acttgcgaag
    27721 cttgattcta tcgaaaaaaa cttcaaaaaa gttcttgaca aataacaaac gataagttat
    27781 gataaatgag ttgctgaaag atttcgaaaa acgaaatcaa aaaaactttt aaaaaagagt
    27841 tgacaattac ttgtctctct gatatgatat aaaagttgct acggcagcca aaaacgaatc
    27901 ggg
//
LOCUS       DANKMN010000038        27232 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000038 DANKMN010000000
VERSION     DANKMN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(161..445)
                     /locus_tag="PW224_001534"
     CDS             complement(161..445)
                     /locus_tag="PW224_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908225.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="PW224_001535"
     CDS             complement(463..1353)
                     /locus_tag="PW224_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL2908226.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="PW224_001536"
     CDS             complement(1373..1591)
                     /locus_tag="PW224_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL2908227.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="PW224_001537"
     CDS             complement(1561..3261)
                     /locus_tag="PW224_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL2908228.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="PW224_001538"
     CDS             3528..4301
                     /locus_tag="PW224_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908229.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="PW224_001539"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="PW224_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL2908230.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="PW224_001540"
     CDS             complement(5125..6096)
                     /locus_tag="PW224_001540"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2908231.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="PW224_001541"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="PW224_001541"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL2908232.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="PW224_001542"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="PW224_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL2908233.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="PW224_001543"
     CDS             complement(9051..9875)
                     /locus_tag="PW224_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2908234.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="PW224_001544"
     CDS             complement(9848..10528)
                     /locus_tag="PW224_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2908235.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="PW224_001545"
     CDS             complement(10551..11489)
                     /locus_tag="PW224_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2908236.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12597)
                     /locus_tag="PW224_001546"
     CDS             complement(11734..12597)
                     /locus_tag="PW224_001546"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL2908237.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="PW224_001547"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="PW224_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2908238.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHLTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="PW224_001548"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="PW224_001548"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL2908239.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="PW224_001549"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="PW224_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL2908240.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="PW224_001550"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="PW224_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL2908241.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="PW224_001551"
     CDS             complement(19463..20704)
                     /locus_tag="PW224_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL2908242.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="PW224_001552"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="PW224_001552"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL2908243.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22654)
                     /locus_tag="PW224_001553"
     CDS             complement(21731..22654)
                     /locus_tag="PW224_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908244.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="PW224_001554"
     CDS             complement(23392..24678)
                     /locus_tag="PW224_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2908245.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="PW224_001555"
     CDS             complement(24671..25939)
                     /locus_tag="PW224_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2908246.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="PW224_001556"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="PW224_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL2908247.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="PW224_001557"
     CDS             complement(26711..27157)
                     /locus_tag="PW224_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2908248.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         7740 a         5873 c         4724 g         8895 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaggtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
    23101 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
    23161 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
    23221 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
    23281 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
    23341 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tc
//
LOCUS       DANKMN010000039        26769 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000039 DANKMN010000000
VERSION     DANKMN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(28..1959)
                     /locus_tag="PW224_001558"
     CDS             complement(28..1959)
                     /locus_tag="PW224_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2908249.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1977..2891)
                     /gene="pfkB"
                     /locus_tag="PW224_001559"
     CDS             complement(1977..2891)
                     /gene="pfkB"
                     /locus_tag="PW224_001559"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2908250.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2888..3640)
                     /locus_tag="PW224_001560"
     CDS             complement(2888..3640)
                     /locus_tag="PW224_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2908251.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3800..4009)
                     /locus_tag="PW224_001561"
     CDS             complement(3800..4009)
                     /locus_tag="PW224_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908252.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4020..5347)
                     /locus_tag="PW224_001562"
                     /pseudo
     CDS             complement(4020..5347)
                     /locus_tag="PW224_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5483..5638
                     /locus_tag="PW224_001563"
     CDS             5483..5638
                     /locus_tag="PW224_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908253.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5712..6299)
                     /locus_tag="PW224_001564"
     CDS             complement(5712..6299)
                     /locus_tag="PW224_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL2908254.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6382..7632)
                     /gene="clpX"
                     /locus_tag="PW224_001565"
     CDS             complement(6382..7632)
                     /gene="clpX"
                     /locus_tag="PW224_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL2908255.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7879..9168)
                     /locus_tag="PW224_001566"
     CDS             complement(7879..9168)
                     /locus_tag="PW224_001566"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL2908256.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9322..10254)
                     /locus_tag="PW224_001567"
     CDS             complement(9322..10254)
                     /locus_tag="PW224_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908257.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10411..11070)
                     /locus_tag="PW224_001568"
     CDS             complement(10411..11070)
                     /locus_tag="PW224_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2908258.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11073..12098)
                     /gene="sppA"
                     /locus_tag="PW224_001569"
     CDS             complement(11073..12098)
                     /gene="sppA"
                     /locus_tag="PW224_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2908259.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12111..12401)
                     /locus_tag="PW224_001570"
     CDS             complement(12111..12401)
                     /locus_tag="PW224_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908260.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12810..14615
                     /gene="glmS"
                     /locus_tag="PW224_001571"
     CDS             12810..14615
                     /gene="glmS"
                     /locus_tag="PW224_001571"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL2908261.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14687..16042)
                     /locus_tag="PW224_001572"
     CDS             complement(14687..16042)
                     /locus_tag="PW224_001572"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL2908262.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16066..17235)
                     /locus_tag="PW224_001573"
     CDS             complement(16066..17235)
                     /locus_tag="PW224_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908263.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17232..18119)
                     /locus_tag="PW224_001574"
     CDS             complement(17232..18119)
                     /locus_tag="PW224_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL2908264.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18308..22000)
                     /gene="nifJ"
                     /locus_tag="PW224_001575"
     CDS             complement(18308..22000)
                     /gene="nifJ"
                     /locus_tag="PW224_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2908265.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22199..22720
                     /locus_tag="PW224_001576"
     CDS             22199..22720
                     /locus_tag="PW224_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2908266.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(23419..23976)
                     /locus_tag="PW224_001577"
     CDS             complement(23419..23976)
                     /locus_tag="PW224_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908267.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(24155..24766)
                     /locus_tag="PW224_001578"
     CDS             complement(24155..24766)
                     /locus_tag="PW224_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908268.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(24879..25136)
                     /locus_tag="PW224_001579"
     CDS             complement(24879..25136)
                     /locus_tag="PW224_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908269.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(25733..26011)
                     /locus_tag="PW224_001580"
     CDS             complement(25733..26011)
                     /locus_tag="PW224_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908270.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(26045..26296)
                     /locus_tag="PW224_001581"
     CDS             complement(26045..26296)
                     /locus_tag="PW224_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908271.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         7559 a         5767 c         4585 g         8858 t
ORIGIN      
        1 gaaaacccag cctcttcttt tttatttcta tgcttctgct actgcaggag cttttctcaa
       61 taaaccataa atcacaccag atacgactgc tccgatagca ataaacacca aatacatgac
      121 cgggtgactc agtaagaata caacgaaaat cccgccgtga ggagccagta atttgattcc
      181 gaaagctccg actaatccgc cagttaaagc agaaccaata ataaaacttg gaatggcacg
      241 aatcgggtca gctgctgcaa atggaatcga tccttccgtg acaaacgata gtcccatcac
      301 gatgttcgtc aaaccagcat cttgatctgt tttgctgaat ttattcttaa acaaacgtgt
      361 agcaacgaaa attgccaatg gaggtaccat tcctccagcc ataacagaag ccatcacgac
      421 acttccgcca gttgcaacag tcgtagctaa agttgctgta ccaaacacat aagcggcttt
      481 gttgattgga ccgcctaaat ctgccgccat cattccagct aataatgcac caagtaaagc
      541 agcattcgaa ccactcatac ttgtcaagaa accgttcaat gcatcattga ttgctttcat
      601 agggacattg accagcaaca tcaagaaacc agtaatcagt aagccaaaca ctggataaaa
      661 gaggatcgtt ttaattccat ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa
      721 tacgatgaca taaccagcta agaatccacc aatcagtgca cctaagaatc ctgcaccgcc
      781 tgtattagct aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt caccgatact
      841 tgaagcaata aatcctgcta ataccggcag catgaaacca aatgcagctt gtccgatttg
      901 gttgaaccag ctagcagctt gattgtaatt ccctagattt gataattgat catgtggcac
      961 gccaataaat tggtcgatca tgaaagaaag cgcaatagcg attcctccac caataacgaa
     1021 aggtaacatg tgtgatacgc cgttcatcaa atctttgtaa attctttgtc cgatagtacc
     1081 atccgcattt ccttcttctg aactgctttc ttttccatca ccatggaata ctggtgcagt
     1141 accactaagg gctagattga tcaactcttc tggtttacgg ataccatcac tgacaggacg
     1201 attgatcagt tcttttccgt caaagcgatt catctctact tttttgtctg ctgcaacgat
     1261 tacaccttct gcacgggcga tgtcttcagc agtcaatcgg tttttgattc cttctgaacc
     1321 gttcgtttcg actttgatct ccacacccat ttctttcgct tttttcttca aagcatcttc
     1381 tgccatgtaa gtgtgagcga tccctgttgg acaagctgta acggcaacaa cgaattttct
     1441 ttctggatga gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc
     1501 tttttgctct tcagctgttt ggaacaagtc ttggacttct tctggtgtat ttgcttcttt
     1561 taattttcct acgaaatctg catcaatcag caatcgagat agtgctgcta atgcttgcaa
     1621 atgtgtgtcg tttgcgcctt ctggagctgc gatcatgaaa aacagataag ttggctgtcc
     1681 gtctaaggct tcataatcta ctcctttttg gctttttgcg aataaaactg ttgcttcttt
     1741 aactgcactg tttttcgcgt gaggcatggc gattccatca ccaagacctg tagaagtttg
     1801 tgcttcgcgg gctagaattc cttctttata tgtttcgata tcggaaatcc ggccggcatc
     1861 aaacattttt tggaccattt catcgattgc gcctttttta tcagtcgctt gcaaatccat
     1921 gatcatcgct tctttgacca ttaaatcctt gatattcatg attatcctcc ttgaaactaa
     1981 tcaatttttg tgatttcaac ttctggcagc aactcatcaa tcaattcgcg agctgccaag
     2041 tcatcagaaa aagctgttgc gcttccgcaa gcaactcccc atttaaaggc ttcgattgga
     2101 tctttcgtac ggctgtaagc cccaataaag ccagcaatca ttgaatcccc tgcacctact
     2161 gaatttttca atgggcgaac taatacattc gttttataag ttccttgttc cgtgaagagc
     2221 aaagcaccgt ctccagccat tgagatcagc acattctgtg ccccttcagc tagtaatttt
     2281 ttcccatacg gaatgatttc ttctacagat tggaaggttg tgttgtaaag ctcagctaat
     2341 tcgtgattgt ttggtttgat caacaacggt ttcttaacaa gtgcattttt taggtcatca
     2401 cctgttgtat caatcacgaa atctgcacct ttttctcgaa tgatatcaat cagttcttga
     2461 taaaatcctt ttcgcaggct agctggcgcg cttcctgaca atacgacgat atctccttgt
     2521 cctactgttc ctaatgcttg cttcaactca tcgatttctt catctgaaat agatgggcct
     2581 aagccattga tctcagtttc agtgtctgat tttagtttga tattgattct tgtatcttct
     2641 tttacggttg tgaacaaaga ctggatttgt tctttttcaa gccacttcga aataaattcg
     2701 ccagtgaacc ctcctaaaaa ccctagtgct gtagattgag aatcgatccg tttcaaaata
     2761 cgggaaacat tgatcccttt tcctccaggt aacttgaagt cttccttcat tcggttcaaa
     2821 tcgcctagtt ttaacccatc tacgcgtacg atataatcaa ttgatggatt aagtgttact
     2881 gtgtaaatca ttgtattgcc tccttaattg ttgtcttttc acggaattca ttaacgaatt
     2941 ctaatggaca acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg
     3001 acaactggtt gaatttggta tgatcaatca atacaaatgt ctcttcggaa tttttcatgg
     3061 ctaaacgttt aacagctgcc tcttctggat ccggagtcgt gaatccttgt tcaagatggg
     3121 caccgttcat tcccataaat gctttattaa aatgaaaatt ttttagttgt tctatcgcat
     3181 tagatcctaa aacagcatta gttgccagct ttatcgttcc acctaaaata atggtagcaa
     3241 gctccaaatc aactaaattt gccgcatgtt gaacggaatt tgtcactact gtgatctgtt
     3301 ttccttgcag aaaaggaatc atttctaacg tagtagagcc agcgtctaaa taaatgacat
     3361 cttcgtcatt aatggtttca gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt
     3421 tgagtgattt ttcactcata ttctgctcaa aattcaataa ttgctctttc ttagctccgc
     3481 catgaactct ttctagaaac ccactattct ccagctcttg taaatcacgt cttatcgttg
     3541 attcagaagc attcaaaagg tttacaagat cttgtgattt cacgattttc tgctgttcaa
     3601 gcaagtctag gattttttgt ctgcgttcct ctgtaagcat tttcacgatc acctcaatac
     3661 aaataatagc atattttttt tctactagca atataaatca gtcaaaaacg ttcataaaaa
     3721 ttataaatca gtcaaaaaaa aaataaaaaa ccagtcagaa agactggttt tttatttttt
     3781 tgtctatcat caagccgttt tatcgttttt tattttcgct tcttgttttt ttcatataaa
     3841 cagaagcagg ttttctttcc ttatgaagaa gataataata aagtcccatc ccgccaaaca
     3901 tgaccaaaaa accgatgact gatcgaatag gaaaattagg aacaaaaaag agacagacgg
     3961 ataataatga tgccggaacc attattcgat aatagagttg tttatccatt ttcttcacct
     4021 catatttttt gtaaagtaat caattgttct aattctcggt aatctaaaaa ttgcacacgc
     4081 agatgctgct cacctttctt gacatcgaat tggatacttc gccgcttttt ctttattcgt
     4141 ttgaaggtaa catcagagag tctatgtttg atttccttta acggtaattc tgacagggat
     4201 tctgaaggaa catagattct taaggataaa agatactctt gatatttttc atctgttacc
     4261 tctacttcta gatattccct aatataataa caataagctt cgattccatt atccaaagca
     4321 acagtcggat cgataacttt atagcacatc atgcgtgatc gattctcgtc aacccaagga
     4381 aaatacgcct gagcatttag tgttccgcga acacgccttt ctttataatc tccttgttgg
     4441 atatcataac tttttgttac aaaaaatgaa gcattctttt taggaacagg ttgcatttgt
     4501 gcattctgat actggacaga ttggatcgta tggtcagtgt aaatgtattc aacgaatggc
     4561 ggtttcaata aaccgacacc attgtcccaa gcaagatgat caaacccaag ctggtcccta
     4621 aaataggatt gaaacatttg attgtttggc ttttgccagt taaaggggtc tcgataaagc
     4681 tggatacata tgacttcgtt ttctaattcc ttcacttttt ctacttgttg gatgtcgaga
     4741 aaaatcggtt tagggatgat ttgatgataa tagcgactat aatacatttc ccaacacgaa
     4801 gtgaaacata aaccttggta aaaaagatcg tatcctggaa attggttgca atcgactact
     4861 acttccccgt tttgttgttt cattttaggt aaatgttcga tttcatcctt tttctcaaac
     4921 aatagccgtt gagagatctg ttttgtattg tgatagataa actcgctata agctcgcata
     4981 taggcgaaca ccccatgagc aatcattttt ttctgaatga agtctcgaat caaaaattgc
     5041 ttttctttga aaacagagat agacaataca ttttttatca ataattgatt atcttccaag
     5101 cgaaaactac attcgttctc gtcatttcct acaactaacc aactttcgtc tcttaccgat
     5161 gctagcatta ttttttcttg aagctttcga agatcatacg gatccgcttg ttgtgtgaaa
     5221 gagcggataa atgttggctg gatcaacaga cgtgtcaaaa tagcccaatc gcttatttcc
     5281 tgtttttttc ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc
     5341 caatcatttt ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga
     5401 aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa
     5461 aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa
     5521 acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga
     5581 tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt
     5641 taagctcaaa aaaagactta ccagccaaac ttttatgaaa gagtttgact ggtaagtctt
     5701 ttttattctt tttatgattc aattgcttgt tcgattgctg cccacgcttc ttcttttccc
     5761 tgttttgtaa cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc caacgctttt
     5821 ttgatttcgc tctcatgctt gttccattta ccacgaggga tcttatctgc cttagtggca
     5881 acaacaatta cagggatatc ataatacttc aaaaattgat acatctggat atcttcttga
     5941 gaaggcttat gacgcaaatc tactaaagaa acgaccgcac gtagctggtc tctagtcgtt
     6001 aaatatgtct cgatcatctt tccccatttt gctcgctcag ttttggatac ttttgcatac
     6061 ccatatcccg gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa attcaatgtc
     6121 tgggtctttc ctggcttacc ggaagtacgg gctaaattct ttcgatcaat caatgtgttg
     6181 ataaaagaag atttccctac atttgatcgt cctgccagag ctatttctgg caattgtgta
     6241 tctggatatt gttttggtgc cactgcactt atgataattt cagcttgatg gactttcatg
     6301 gtctctctcc ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag ccaaaatgac
     6361 ttttacggca gcctcttttc atcaacccgc ttttttctct gaatctttat agattactgt
     6421 tggctcacca gacaattcag ctgcttcttt tgtgataatc actttttcga tgttttcatt
     6481 tgaagggata tcaaacatca catccatcat gatttcttca atgatggaac gaagtcctcg
     6541 agcaccggta ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt caggttcgaa
     6601 ttcaagttcg gtattatcta gagaaagaag tttttgatat tgtttgacta gcgcattttt
     6661 cggctcagtc aagatccgta ccagatcatc tgttgtcaat ttctctaaag ctgccataac
     6721 aggtaatcgt ccgataaact cagggatcaa tccgaatttt agtaaatctt caggaatgat
     6781 atgctgcatg acactttcgt cttcagaaag tttttggttt tgtgtaccaa atccaatcgt
     6841 cttttcgccc atacgattct tcacaatcgt ttcgatgcca tcaaatgcac cacctacgat
     6901 gaacaatatg ttcgtcgtat caatctgaat aaattcttga tgtggatgtt tacgtccgcc
     6961 ctgaggtggc acacttgcaa cagttccttc tagaattttc agcaaggctt gttggacacc
     7021 ttcgccggaa acgtcacgag taatagatac attttcgctt ttacgcgcaa ttttatcgat
     7081 ctcatcaata tagatgatcc ctttttctgc aagttccaca ttgtaatcag cagattgaag
     7141 caatttaagc aaaatgtttt ctacatcttc ccccacataa ccagcttctg tcaaacttgt
     7201 cgcatctgcg attgcaaaag gtacattcaa tgtttttgct aatgtttgtg ctaaaaatgt
     7261 tttaccagac cctgtaggtc cgatcaagca gatattgctt ttttgcagtt caacatcatc
     7321 tgtgttttct tgtgcttgct gattgacacg tttatagtga ttgtatacgg caacagctaa
     7381 cgagcgtttt gcgcgatctt gtccaatcac gtagttattc aatacttcaa gaatttctag
     7441 tggtttaggg acttcagtga attcgcgtac agcttcttca taaaattctt catcaatgat
     7501 ttctttacat aggtcgatac attcattaca gatgtagacc cctggaccag ctacgatctt
     7561 tttcacttct tcttgggttt tcccgcaaaa agaacagcgt acggtctcat tgctacttgg
     7621 attgtcatac atggtcttca ccccttaact tgcaaaaagt atgcaagaaa ctatcaaaga
     7681 ctttacagtc ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt ttaacaaagg
     7741 atcaactgac ctataccttt gcatttttta ctcttaacaa aaaataagga actgtgacaa
     7801 aatcgtgtca cagtccctta tttcgaataa acaattttct agaagtagct tcttcagaaa
     7861 tgatttatta cttgatttct attctttcgc ttctgtcgct gtacctgtga tcaattcgat
     7921 tgcttttttc attgtcacat catgttccag catatcttct gtcaacacgc gtttgatttg
     7981 ttcgattggc atgttgtatg tttctgataa ttcttggatt tctgcattga tttcttcttc
     8041 tgttgcttga agattttcag cagctgcaat tgcttcgatc acaagatttg ttcttgtgcg
     8101 catttctgct tcaccttcga attgtttatg caagtcttct tctgtagaac cagttaattg
     8161 atagtacatt tcaggagaga taccttgacg ttgcatgttg ttcaagaatt cgtccattga
     8221 acgatgtact tcatcatgaa ccatcacgtg tggcaattca acgatttctg cgttttcaac
     8281 agccatacgg atcgctgctt catcttttgc atcgtccgct gctttttctt ttgtttctac
     8341 taattctttg cgatattttt ctttcaattc gtctaatgtt gatacttcgt catcaacatc
     8401 ttttgcgaat tcgtcatcta attcaggcac ttcttttgct tttacttcat ggattgtcac
     8461 tttgaagact gcttcttttc cagcaagatc ttctgcttga taatcttctg ggaaagttac
     8521 atttacatct agactttcac ctgatttatg accaactaat tgatcttcaa aacctggaat
     8581 gaatgagcct gaacctaatt caagtgagta attttcgcct tttccgccat caaacgcttc
     8641 gccatctaca aagccttcga agtcgattac tactgtatcg ccattttcag ctgcagcatc
     8701 ttctttgata actaattcag cttgtgcttc ttgttcacgt ttgatacgtg catccacgtc
     8761 agcatctgtt acttcacggt cttgtttttc aacaactaga tctttatatt gtcctaattt
     8821 tacttctggt ttcactgtta cttcagcagt gattacccag tcttgacctt tttccatgct
     8881 ttcaacatcg atttttggtt gagaaacagg atcgatgcca gcttctttga cagcagcttc
     8941 ataagcttct ggtaatacag cattcaatgc atcttcatat aaagcttctt caccgtacat
     9001 acggttgaat acttgacgag aaactttccc tttacggaat ccaggtacgt tcaagctttt
     9061 tttcacttta ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg caatcgtaaa
     9121 ttttaaaaca ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt aattccctcc
     9181 gagtttagaa attcatttta tacatcaaaa acgtttcatg cataccttta aatagtacac
     9241 taaccctttc aaattgtaaa catattgaat ggatagaatc cttgtttttc ataacatttt
     9301 tatctttttc actgataaaa tctaatggaa gaagatcatt tgctgcaaaa ttttttctct
     9361 tttttttaaa aaatcttgtt tttcatcttc tgtaccttca tacatcgttt ttcctgtaca
     9421 cgcctctaca gtcagttgaa accagcgctt cgggtcttcg atgtatgtat catagacagg
     9481 ataaacaatt gcagattgca acgtgaattc ttcttttaac acggaactaa gcagcgcatc
     9541 ttcattcccg taacgatctg aaaatacaca taattgctgg taacttgcct gctgctgcgg
     9601 ctttggcaga tcttttggta tgactttgac taacttttct tcaatcgtca aatagaaaac
     9661 aggatggcat tcgttcaatt gcctaagtgt ctccagtaat tttgcccttg ctaaaatatg
     9721 gacctctggc gatacaataa gtttactcgc tgtttggata aactcatgat aaggaagctg
     9781 ttcgatctca tcaatagacg caagctgacg tgcaggttcc aacgaaggaa gttcctccga
     9841 tttattgtgg agtgccttga tcttctgcct ctccatttgt ccggataatt cttctgcaaa
     9901 ttggattttt tcaatgaccc gctgctcgaa agcttcataa aaatctgtac ttgctatcaa
     9961 ttttctagca tataaaaaat cttgtgtact gattaacaag tcaaaataaa attcagcaaa
    10021 ctcctcattt tgaagatact gtttttcatg caagagtgct tgcttcaatg cttctttttt
    10081 ctcgtccaac tcgtaaagac aaaaaacaag caggctatta gcctcaaagc tttctttcaa
    10141 ctcgtaagct cgttgaaata attcttttgc ctgctgatat tgctggtttg ctagtgcttg
    10201 ttgtgccact cgcattaaat tctcatattt ttctggaaaa tcaatcggct tcacgcagtg
    10261 tcctcctctt gctcatattc agctcatgtt caatagtagc aaaacaaaaa aagcatgaca
    10321 attaaaaata actgaagcac cctctaaaag ctagatttta gatctgactt ttagaaggtg
    10381 ctttcaactg ttactgcttt ttattgctca tcagctgttg acttcctggt tgaacgcttt
    10441 gatcagattg atcgtaacaa cacttgtatt agtgaaaaaa tcgcccagat gttgcttttt
    10501 ctgattaaag actgctggaa gataccctaa catcagcaaa ggaaatttta gaatgaaccg
    10561 gcaagcacct tctctaacta aaacggttga ccagctaagt tcttcttcat caaagctgat
    10621 gactcgaata ccaaagatca ttttcccaat cgtttggcca tgattgaatt tcgtcaataa
    10681 aataaaatac gctagataaa taagtaaggc tgtgaatccg tagagactca gataagaatc
    10741 tgaaaaatta agccaaccta gatttttggc aataccaagt gttaaacgcg taatagctcc
    10801 gatacacaaa agatcgacca agaaggcaaa cattcggata aaaaaacctg catagaaata
    10861 cttaggaaaa tcatttgctt ttggcggttc atttcttgtg tccgtatact ttttccattt
    10921 acgttgtttc tttttgattt cttcgggcgt caaaggctct ctttcaaaag agctcagtac
    10981 cttttgggaa aagttggagt caggtcctac agcaggctgc tgttgcttat ctttttcttg
    11041 ttccttattt tcatttgaaa gctctgacat gcttactcac ctccgtaata atacattgct
    11101 tttggtgatt ccgctgtccc tagattctca agaacggata agatctgact agtctcagaa
    11161 ggcttcaatc cctggaattc tgctagttta cttcctaacc atgtactagc aaacccagat
    11221 ggcgtggaag aatattctac tagttccgca tcttcaagct gttgttcctt tcgtatagct
    11281 tttaaagcat cttcaggaaa tcccaattca tcgactaaac ctacttcttt tgcttggaca
    11341 ccatcataaa tccttccatc agcaattttt ttgacttctt cttcagattt attccgtccc
    11401 tcgctgacga ttgctacaaa acgactatat gcgctgtcga tatatgcttg aagcaccgct
    11461 tcttcttctt ttgtctctgg tcgcgttcct gaacccatat ctttcaacgc tccgctttta
    11521 actgttgtat cttccacacc cagtttttct agaagtcctg aataattcaa tcctgacatg
    11581 atcacaccaa ttgagccggt cactgtttct tctgttgcaa agattttatc tgcttgagca
    11641 gaaatatagt agccaccgct agctgccatg tttttcatgc tgacatacat tgggatatca
    11701 tgttctttac gaatcgtatc caatttctta gcgatttccg cactttcgta gacaccgcca
    11761 ccaggagaat ttacttctaa aaagatacct ttgactgttg gatcttcttc aatcgccttc
    11821 agttgttcca taaaattttt atgattgtaa ccttcagaag caaataaacc tgaagaccct
    11881 cccgcagcaa tcgttccatc tactgataat tttacgattt tttgcgtact gtctccctct
    11941 tccaacacat ttggagctag ttcttcatcc ccatataacc aactgttcaa tccctcaatt
    12001 tgatcgtttt cctgaggctt agtcaaacta gctgaaacta acgaaaccac aaataatcct
    12061 gcagcaatca gaactgctgc ccaacgtctt ttattcattc tttctccttt ctaatacaag
    12121 ttttttatga cgatcacagt ttctgactgc tcagaagtgt cgaaccgctc tgctttttct
    12181 tcccgcgcat acccggaaaa cgactctttt gttaattcat cttctaccgt aaactcacga
    12241 gcttgagcaa cttccaaggc atcaggtgcg atttcttgga tgctatccca gccagtatct
    12301 aaaaatattt tcgtatcatc tgatacacca gctgatgctt cgaatttgga caattgtttt
    12361 tttaacattc ccagtgtcat gatctcaatt tttgaatcca tttttactcc tcctctatcc
    12421 gattactttc tattgtaccg aactcctgaa aaagatgata gataaaaacc cattattttt
    12481 tgacataaaa aggacatttg acaaagaatt tgatatatac caatttattt tttattcttt
    12541 ttttgttgac aagaacattc gcaaaaggtt atgattttaa atgtttccag ataaaaaaca
    12601 aaaaggagga tagttatacc ttttaggata gcgccaggtc tgtgtaaaca gatgacgagg
    12661 agagggttta tcgaaagatt cggcggatgg ccctagggac tgcactctac agaaaatcca
    12721 caaaaaatgt ctgcaaagat aaaacaaaag gattttcctg cagctggaag cattcatacg
    12781 aattcattat agaaagtagg atgtttttta tgtgcggaat tgttggaatg attggattaa
    12841 aaaatgtaac accaggattg attgacggtc tagaaaaatt agaataccgt ggttatgatt
    12901 cagctggtat ctttgtctca gatggcacaa ctgattatct ggtcaaagca caaggcagaa
    12961 ttcaaaattt gaaaaacaaa ataaatattg atactacagg aaatataggt attggtcata
    13021 cacgatgggc aactcacggg caaccttctg aagaaaacgc ccatccacat acttcccaaa
    13081 gtggacgctt cgtgctcgtt cacaacggcg taatcgaaaa ctttgaagaa cttaaaattg
    13141 cttatttagc aaatgaccac tttattggtg agaccgatac tgagattatc gctcatctaa
    13201 ttgaaacttt cgcgaaggat actacaacgc aagaggcgtt tttaaaagct ttgcgggtca
    13261 tcaaaggttc ttacgctttc gctttgattg atcggacagc acctgatgtt atttacgtcg
    13321 ctaaaaataa aagtccacta ttaatcggac tcggcgatgg attcaatgtg attgccagcg
    13381 atgcaatggc tatgcttgct catacgaagg aattcgttga gattgaagat gaagagatgg
    13441 ttaccgtcac atcagaaaaa gttattattc aaaattttgc aggtgacatc gtagaacgtt
    13501 cttcattcga agcacaagta gatgcttcag atatcgagaa aggtacctat cctttttata
    13561 tgctgaaaga aatcgacgaa cagcctatta tcatgcgtcg tatcgctcaa aaatacgtaa
    13621 cagaagacaa tcgtgtctct cttgatcaaa agctggtcga tacattgtct gatagcgatc
    13681 gaatctatat cgttgcttgc ggaactagtt accatgcggg gttagcaggt aaacagactt
    13741 tggaaaaatt gacacagatt cctgttgagg ttcatttagc cagtgaattt ggctataaca
    13801 cacctcttct ttcaaaaaaa ccattcttta ttttcttgag tcaaagtggc gaaacagcag
    13861 acagccgcca agtgctggta aaaatcaacc gtctcggcta cccttcactt acgattacaa
    13921 atgttgctgg ttctactctt tcgcgggaag catcctttac actgctattg catgctggac
    13981 ccgaaatcgc agtagcatca acgaaagctt atactgcaca aatagctgta ctaactttat
    14041 tagccaaagc tatcggtgat aaaaaagaat ttgaaacttc tcttgtattc gatatcactc
    14101 atgaattgag tttagttgct aatgcgatgg aatcggtcat cgcccaaaaa gattatttag
    14161 aagagttggc agaagtatat ctcagcgata caagaaatgc cttttacatt ggacgtggtg
    14221 ctgactacaa cgtttctctt gaagctgctt tgaaattgaa agaaatcagt tatatccagg
    14281 cagaaggttt tgccgctggc gaattgaaac atggaacgat tgctttgatc gaagaaggaa
    14341 caccagtgat cggaatcatc agtgaagaag tgactggtgc gcatactcga ggaaacctga
    14401 aagaagtcga aagccgtggc gctaaaacac tagttattgt ctcagaggga ttagaaaagg
    14461 aaagtgacca gctggtgcta ccaactgtcc acccttatct aactactcta accacagtcg
    14521 taccgactca attacttgca tattacgcaa cgctattacg gggattcgac gttgacaaac
    14581 cacgtaattt agcgaaatca gtgacagtag aataataaaa gaacaaggac cagtgtcaga
    14641 catctgacac tggtccttgt tcttttattt tttatgaaaa tatctcttaa tccagtccga
    14701 tttcttcttt gactactgcc gcgattttat cgacatagta attaactttc tcatcagtag
    14761 gcgcttctgc catcactcga agtaatggtt ctgtaccgga aggacgaact aggattcttc
    14821 cttctgatcc catttcagct tctgcctctt caatcacttt tttgattgcc ggaacatcca
    14881 tcgcaccgtt tttatcactt acgcggatat tcactaattt ttgcggatag attgttacct
    14941 ctgctgcaag ctctgataat ttcttacctg tctgtttcat gatattcagt aactgaattc
    15001 ctgaaagcat cccatctcca gttgtgttaa aatctaagaa aatcatgtgc ccagactgct
    15061 cgccgccaaa gttataatcg tttttgcgca tttcttctac aacgtaacga tcaccaactt
    15121 gtgtaacgac atctttcaaa ccgattcctt ccaccgcttt gtggaatcct aagttactca
    15181 tgacagtcgt aacgatcgta tcttttttca atcgattttt ttctgccaag tatttagcac
    15241 agataaacat gattttatca ccgtcgacaa tgtttccaag ttcatccacc gcaataatac
    15301 gatcgccatc gccatcgaaa gctagtccag catctgctcc tttttcaacg accatctcag
    15361 ctaaacgttc tggatgggta gagcctacgc cgtcattgat atttaacccg tctggatttg
    15421 tgcccattgt ataaaaatct gtttcaagat cggcaaacaa acgattaaca cttgttgaag
    15481 ttgctccgtt tgctgcatcg acacaaatgg tcaaaccgga aaggtcaccg ttgatcgttt
    15541 gtacaaggaa ttgagaatat ttcaataagc cttctggaaa ttcttctaca gttcccaatc
    15601 cttcagctga tggacgaggc aattcatcca cttcagcatc taataatgct tcaatttcta
    15661 gctcttggtc atctactagt ttaaaaccat ctgcaccgaa aaatttgatt ccattatctt
    15721 gtgctggatt atgagaagca gaaatcatga cccctgcgct tgctttttgc aaacgtgtca
    15781 gataagcaac acctggtgta gagatcacac ctagctggaa cacttcgata cccaccgata
    15841 aaagtcctga aatcagcgct tcttctaaca tttgacctga gattcttgta tcccgcccta
    15901 ctaacacgcg tgggcgtttt ccttcttctt catgctggct taggacataa cctccgcacc
    15961 gtcccagctt aaatgcaagt tctggcgtaa gttccttgtt ggcttcaccg cgtacgccgt
    16021 ctgttccaaa atatttaccc atagttgtaa aattactcct ttaacttaat tttctgagga
    16081 atttgttctg ctcatttgtg ttgattcgct tgtttctgat gtttcttctg tacttgtggt
    16141 cgagctgctt gtcccagatt ccgaactgga agaaatggtt gttgtcgatg gttccgttcc
    16201 tgaatcagca gatgaaatac ttctactcgt acttgtattt tcttgtgtcc gactgctcgt
    16261 cgaagcagag ctactgcttg acgtttgcaa cacgggtgta atctccacac taactgattc
    16321 gggcttgata cttaacccct cttctactgg tatttcaatt ttacgagttg ttcgatgatt
    16381 gatacctgag acattgatcg gtacagcaat actgtcaccg atcgatgcaa gcaaatcgct
    16441 agagccgctt acttctgctt ccagttttga aaaactgaaa tcatagcttt ctaccccggc
    16501 agctggtgtt ccttgctgta tcccataaag tcgtaccgac ttgcttggct tgatcgaatc
    16561 agctctcact gttacttgca ctggatcaga gacgatactt aatacttccc ctgctgcatt
    16621 cagcgcctgt attgggacct cactattaat cccatcatct gtgaccatcg aagcgtccac
    16681 atcagctact actcgtttga tttctgccaa tgttttgtca cctgtagtga tagagacttt
    16741 tttaggacta acggaagtat catcaagttc atatccagat ggtgtgaacg ttgaaggaat
    16801 caacgtttcc acatcgaaat cttttattac ctttttctca accgttactg taatagtcga
    16861 tggttcgatc tttgctgtga cagcactgct caaattttga atttttagcg gaacttcatg
    16921 tgttccttcg cccagcttag tgatatctgc tgtgacgcgg aacatccgtg tatcttcgtt
    16981 tgcctctgca ttcaactgaa ctcggtttgc actggaaagt ttgacagtca ctgcattttc
    17041 atatccatgg atatagtagc gctttgaatc ataagaaatc ttgatcggca cccccgtaac
    17101 tgtttgatta tacgcttcat ttccagaaag cgtatttgga acggactggc catttgcatt
    17161 gaagtacagc gcaatactga acagcaatgc aataaaactg taaagaatgc ttgtccgttt
    17221 ctcttttgag atcatttgcg atcccctccc ttagtcatac cgtctaggaa agattgaatc
    17281 acgtttttct tgttttttgt tttctcttca gcaattagtt ctctacgcaa gattgccaaa
    17341 tattcttgct gggataagcc agccatcagt tcattatcta acgtgatgct gacgtccccg
    17401 gtttcttcag agacaacgat ggtaatcgcg tcactgactt cgcttatacc gacagctgcc
    17461 cggtgtcgag tcccgaattc tttagggatc aacatacttt ctgatagagg aagataggcg
    17521 cttgctactg cgatctttcc ttcttttaca ataactgcac cgtcatgcag cggtgtatta
    17581 ggaataaaga tattgatcag cagctcccca gtgatatctg catctaaagc aatccctgtc
    17641 tcaatatact catctaatcc agtatgacgt tcgatagtga tcaacgcacc gatttttcgc
    17701 ttggacatat attggatggc tttgtcaaaa gacaagatca tcttctcatc ttcctgctgt
    17761 tcgctttttg attgtttgaa aagcgaactg cgtccaagat gttccagtcc tctccggatt
    17821 tccggctgaa aaatcacgat agcagctatc accccgtacg taatgacttg gttcatcagc
    17881 caagacaagg tgtgcagacc aatgatttcc gctacgattc ggataacaat aaaaatagct
    17941 acacctttca gaagctgaat cgcttttgtg cctcgcagca gttggatcag tttgtataca
    18001 agataccaga caaccagaat atcaagtatg ttcaccaaaa aattcagaga gaacaagtca
    18061 ccggaaaaca tttgctgcca atagtccaaa ttaaaaagtt ccccgatttg aaatgacata
    18121 aaaagcctct cttctctttt gagatttcct ctctagtata tcatatgttt taattttaga
    18181 ggctgatatg ctcgtatttt tcttgagaaa aacgcataat tttcaagaaa attgcaagta
    18241 tgcgttttat ttttctctgc aataaatatt tttaaacttc agagaattat tcattatatt
    18301 ttttctgtta tttttccttt ttttcttttg tagcttgttc cattttttta cgtcgttctg
    18361 ctctggcagc tcttctagca gctcttcttt cttctgcttc aaggtccgtc gttttttctt
    18421 ttctagtctt aacgggtaca tcgatagatt cctctggttc aagtcgtttc ttgatttttt
    18481 ctaattctcc cgtcagtgca gcatagttgt aaaaacgtgt tttagcgtcg tggacagtct
    18541 tttcaaataa agcttttgca agatttggat ttgcgttatc tagcgaggaa aagcgaactt
    18601 gtttcctcat gaaatcaggc atagcagaga aatcaggttt tttgtagtcc aaagtcattg
    18661 ggttcttgtt tttttcgacc attgtagggt tatatcgata taatgaccaa taaccagaag
    18721 aaacagcttc ttttgcttct ttaagcgatt ctttcattcc gccaagcaag ccatgtgtga
    18781 tacaaggcgt ataggcaata atcagagaag gaccaggata cttttctgct tcttcaaatg
    18841 ccttgatcgt ttgcatttga ttggcacctg aagcaatttg cgccacataa acatttccat
    18901 atgtcatcgc catcattcct agatctttct ttgaagcata ctttccacta gccgaaaatt
    18961 tagcgattgc cgaagctggt gttgcttttg aggtttgacc gccagtattg gaatagactt
    19021 cgttatccaa aacaaggata ttcacgtctg ctccgctggc aagtacatga tcgatcccgc
    19081 caaaaccaat gtcgtaagcc catccgtctc caccaatcat ccattggctg ttttttacga
    19141 atagatcctt tctcttgtat atatcctcca gttcagggaa aattgatcgt tcgctttcga
    19201 tcgcagcttg cagttttgct gctctttgct gcgttccttc actctcaaac atgtgtacta
    19261 accagtcttc catcaattct cgtagttctt ctgatacttt tggcaatact tctttgattg
    19321 aacttgccaa agcctcgcga cgagtctgtg ccgctagaag cataccataa ccaaattcag
    19381 cattatcttc caataaagaa tttgaccatg caggcccttg accacattca ttggtcgtat
    19441 aaggagaagc aggagaagca ccgccccaaa tagaagaaca gcctgtcgca ttcgcgatca
    19501 tcattcgatc accgaacatt tgtgtcaata attttacata aggtgtttcg ccacagcctg
    19561 agcaagcgcc cgagaattca agaagcggtt gattgaattg tgtagacaag acagtattct
    19621 ttcctttgac tggatttgct ttttgtctta atgtcattgc aaaagcccaa ttcacagctt
    19681 gttctttctg ttcttcataa ggcttcatca ctaaagcttt ttcttttgcg ggacaagcct
    19741 ctacacacag accacaacct gtacagtctt ccactgaaac ttggatacgg tattttacgc
    19801 cgtctgcgcc tcgcatttct ctcacgataa atccttctgg cgcttcttcc atttcctctt
    19861 catctgctaa aaatggacgg atggcagcat gaggacaaac aaaggcacat tcattacaca
    19921 tcgtacagcg atcactaatc cactcaggta cttctaaagc gacgccccgc ttttccatag
    19981 aagccgttcc taaaggcatt tcgccagttg tcattccatt tgctacaagt gtaccaacag
    20041 agagttgatt tccttcctgt gcattcactg gttcaagtat ttcttgtata taaccagtgt
    20101 tccttttatt gattttttca ggcatctcca acgttctcca atggtctggg atcgcgactt
    20161 gatgaagcag ttcggtcgtc cgatcgattg cttgtatgtt tttttctacg atagccattg
    20221 atttatgtcc atagcttttc agggcttctt cttttagcag cggaagcacc tcatcaaaag
    20281 gaatgatgtc agctaattta aaaaaagccg tttccattgc cgtattgatc cggcgtccca
    20341 aaccgacttc ttgggccaat ctcatggcat tgatcgtata gaatctaatg ttattttcag
    20401 ccaagtaacg ttttaatttt aagggcagat gcgtttctaa ttgttcgtcg ctccaaagag
    20461 tattcaataa aaaaatgcca cctggcttca atcctttgac caagtcatag ctatgcaggt
    20521 aagctggtgt gtgacaggca acaaggtccg catgctcgac aagatacgct gaacgtatcg
    20581 gcgtatctcc aaaacgaaga tgagagacag tcagaccgcc agattttttc gaatcataat
    20641 aaaaatagcc ttgcgcatat ttatcggtgt gatctccaat aatcttgata gctgatttat
    20701 ttgctccaac tgttccgtca gatccaaatc cccaaaactt ggcctggaat gtttgcggtt
    20761 ctgtcaaatc cagactttcc tttggttcca aagattgata agtcacatca tccacgatcc
    20821 ctatggtaaa ccgttttttg cgtatgcttg ggtctttttt caattcatca aaaactgctg
    20881 agatttggtc gggagtcaca tctttagacc ccaatccgta gcgaccacca ataacagccg
    20941 ggcgaagttc agaatcatat aacgcacttt gaacgtctaa taagagaggt tctccacctg
    21001 cccctggttc tttacttcga tcaagaacag caattgtttt tactgtatga ggcaactttt
    21061 gcacgaatgt ttccactgga aaaggacgat aaagatggat attcaaaaaa cctgttttcc
    21121 gtccgtttgc attcaagtaa tcaatcgttt gttcaatcgt ctgtgcaact gatcccatag
    21181 aaacaatgac ttcttccgca tcgtctgctc cgtaataagt caccaagtca taagaagttc
    21241 ctcgtaatcg gttgatctct ttcatatatt tttgaacgat ttcaggtaat ttatcataat
    21301 aggggttgat cgtttcccgt tgttgaaaat ggatgtctgg attctggttc gtaccactaa
    21361 cagtcggatg attgggattc atccctcgtt tacgaaatga agctaatttc tcctgattga
    21421 tcagaggagc taattcttca tattccaaaa cttcgatttt ttgaatctcg tgactcgtac
    21481 ggaatccatc aaagaaattc aaaaaaggga cttctccttc aatagaagca agatgggcaa
    21541 caggtgctaa atccatcact tcttgtacac tgctttcagc taacatagca aatcctgttt
    21601 gtcttgcagc catcacgtct ccgtgatctc cgaaaatatt caacgcattt gtcgtcactg
    21661 cccgactagc aacgtggaaa acacttggca gtagttcccc agcaatcttg tacatattcg
    21721 gtatcatcaa aagcagccct tgtgaagcgg tatatgtagt tgtcagtgca ccagttttca
    21781 atgatccatg gaccacacca gctgctcctg cttcggattg catttctact acttttactg
    21841 gttgaccgaa tacgttttct tttcctgctg ctgaccattg atctactaat tcagccatgg
    21901 tcgaactggg tgttattgga tatattgcag caatctcggt aaatgcataa gaaatatatg
    21961 cggctgccgt attgccatcc attgttttca tttttcgcat gattcatcat ccttgttcat
    22021 attctctctt attatctgtt ccattataac gtaaaacatg ccattttttt cttttattta
    22081 taaaaatatt ttacatgtat ctatttgaat tggtcatcta atttttagac aaacagtcta
    22141 aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt gttttaacat
    22201 ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc tttatggttc
    22261 atcgaatgct cctaaaaaaa tggtcgaatt catcaactta gcttgtccgt attgccgaca
    22321 atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc aattgcagcg
    22381 agtgatcaaa ttgttcgata aagaaaaaga aagcttacag cgagggaacg tgatgcaccg
    22441 gtatctcaca atcagcgatg gccaaaaagc gatcaaagag atcaaacaaa tattcgatac
    22501 tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag tagataaact
    22561 aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg aagctgagca
    22621 agcacatatt cgatttgttc caactgtcat cttgggtaaa gaaatctttg atgaatctat
    22681 ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataagg agcgtgggac
    22741 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    22801 aactccttct tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa
    22861 tggtgtccaa aagcttgcat ctgccagatt atctcatttc ttatgacaag ctaagcaaat
    22921 tatctccacc aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat
    22981 tttcatacaa aatttctcaa tttcccttaa cgagtaatat ttatgtacgg tgattctcac
    23041 tagtatccca atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct
    23101 tttatgtatg ctgttagttg attagatggt catttgttga gctaggtcgt ttattttaaa
    23161 caagttgttt ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa
    23221 tgcatacgca tctgtatttg ccactttgat tcgtataaag ctaacaacta ttaactcctc
    23281 tcagctaata gttgctctct tttttattca gattcatttt tgctgatcac agcctagtat
    23341 ttcaatggtt ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa
    23401 tggattagta gatttcgatt attcaaagcg attttctatt ggtggatcgt gttcccaact
    23461 aataagacgg attccaaacc tatcgtacag atcttctaaa ccacttataa attttgcacc
    23521 tatttcaggt gttttattgc gtggatattc tattgttacg ccattaaatg gataccaatt
    23581 aggattaggc tctctttttt tctgtacatc tcccatagaa tagttagcac tttctgggac
    23641 ataatatatt cttcgaccat cctctaattc atctaataag atatttcgtg atcgtctatc
    23701 acttgctaaa tgtagacgcc acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa
    23761 tccatcaaat aatgcaacaa ttgtatcttc tatcacaatc ctttccccat ctacttcata
    23821 ttctaatcta aaatcaaact cccctcgggt aatttctggt cttggtggct taggacccaa
    23881 tattattcta cttaaaaccg cattcgttcc cagatgaaac acagctataa caacaataat
    23941 gattaatatt ttgtttctat ttgataaatt tctcattatt cgcacatcac ctctacattc
    24001 tcacatatta gttcgtaata ctcctttaat atcaaaatat ctactggtcg taataatatt
    24061 cgctatgaat tcctactact cgtataagaa aggccaaatc atcagctttt acttgctgat
    24121 gatttggctt gtttctattt atcgtaattg ttttctaatc accaaaattg ttaacaatcg
    24181 gttcagatat ttcccagttg attaaggtaa ttccgtattg tgtcaaaact tcttgcgctt
    24241 ctccgaaatt aagtacagtt cttatgtatc cactttctcc taatataaag tctacctctc
    24301 ttttaaaacc ttcctcgaga tttctaaggg ctatatgtgg ttttaatgac attgattgta
    24361 cattttcccc catataatat tgcgcacttc ctcgactaaa atagattcct agatcgtccc
    24421 caaaaggtag atcttctcta ccactagcta atctacttct ccatcttcgt tctcttttca
    24481 tagaacctgc gctgaactca cacccgttaa actcagctat gacagtatct tcaattatgt
    24541 taatctgtcc atctagttcg tattcaatcc taaatggaaa ttctccatag gtgatttccg
    24601 gttcgggtat tttaggtgag attaatgcaa agaaaggaaa tattaagctt ggcaacatca
    24661 aaaaaactcc tcctatgatc acaattcccg atgtaatcca aaatataatt tgtacaattc
    24721 tcattgatcc attgtattcc attttattcc ccctattact attcatatcc gcctatctat
    24781 atcgatcttc caatgcagag ataattgctt ccataccttg attttttact gagttaccta
    24841 tgcgagatat cattctccac taaggtgcat catattgtct atctaacatg attaattctc
    24901 caataggtgt atactcctcc aaaaacaaca tttaatgcat ctccctcgac aacataagct
    24961 accatgtttg ctgtatatgt gtttgagtct aattcataag cagataaatt tactgtcgct
    25021 ggattaaata taatcgcatt cgtatttata gctactgaat ttgctgcttc agcaccacct
    25081 tttgaatgtc cgatcattgt gatttcaata gtaggattta cgtggacagc cctcaccgtt
    25141 taccctgacc attatagaaa tcgcttactt ttttgaacta cgcaacaaag gatacaatgc
    25201 tcctaacttc actgtctcca tttttgtcca ctaaggaata taaaaaatag gcattaattc
    25261 atttaattcg gtcacaacac gttacctttt aaatcattat actgtatctc atacattatt
    25321 atcaataaac tattataaaa atgcaaaaaa tgctttatca atctattttg taattggtca
    25381 taatatcatt gaaatcatac cctgtatcga tttttcctat gaatctcgtc tatttgtgat
    25441 tgacgaaaaa gaggcaagac agacactgga atggtaattt tacccactat tttgctccaa
    25501 ctatttgtaa tcgtacccac ttttgaaata ccgagatgat cattttaatt gatatgctga
    25561 ttcccaagca aaaccccaag aaattaaatg ctctatcgca tctagcttct tgggttttgc
    25621 ttatataaat tccaacattt aaatttttta tgctgttcgg tctcagaaat gccgtttttg
    25681 cttatatcac aatatcaaga tgtcaaatta ttcgtgttgc cgatgacttt gattaatttt
    25741 tttcaaattg tttattaaga ctaatgctgt ctttttgcag ataccaatgt tcgaactttt
    25801 taacattatc atatgatcct gcaagtttta acttcttacc attagatgtt atcaaagtta
    25861 taattaacct tgcctttttc ccattaggat cttttatttt atgaattttc tgcaggtaat
    25921 ctctcctaat ggcagctatt tttacctcat cagtgctgat aaaccattga tcatccacat
    25981 acatactgcc tgttatgttg tgtttttcca tttcttccga gaacgaaaag tttaaggctc
    26041 gttcttactt ccaaagttga atgagtccat ggattggact tagtgtgaga attgagaaca
    26101 acaataagaa tccatatcct cccgcaaaca tccctaaggc tatactgtct ctccatccat
    26161 aagtaggatt agaagagaaa agagtcaccg tttgtaataa tatgaaaagg aataatccag
    26221 cacaaacacc aaagataaca actccaaaca ttgaattttt atacaatgac catttattta
    26281 taggcttttc tttcatttta cttcccccta ctgttctgtt cacacggaat gaattcaaag
    26341 ttcataagcc ctcctctttg aacatatgaa tacgacttgc taactatact ttaaaaaagc
    26401 ttgttttaag cggttatagg cgagttgtaa tctctattca tctcattagc aaataacttg
    26461 ccgaaaagtc tactgaaatt aaagcagata gtggaagaat tatttagcca ggatagacac
    26521 tatttgtgaa acacatgtct ttacttttga aattctatga tgaaaagcct tggaatacag
    26581 tagctcttaa ttggtaatac tttttccatt ataaataacc tcttttcatt ttatatttat
    26641 cccctctaaa aataatatct aatctaatat tttaaactta tatttccctc gctgtgctaa
    26701 tagagcatta ctaaataaat agaaactaga aaaaagcatt acgcctgcca gaataaacca
    26761 attatttgt
//
LOCUS       DANKMN010000040        26171 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000040 DANKMN010000000
VERSION     DANKMN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            131..3658
                     /locus_tag="PW224_001582"
     CDS             131..3658
                     /locus_tag="PW224_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL2908272.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="PW224_001583"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="PW224_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL2908273.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="PW224_001584"
     CDS             7446..7787
                     /locus_tag="PW224_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2908274.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="PW224_001585"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="PW224_001585"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL2908275.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="PW224_001586"
     CDS             9144..11564
                     /locus_tag="PW224_001586"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL2908276.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="PW224_001587"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="PW224_001587"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL2908277.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="PW224_001588"
     CDS             12998..13735
                     /locus_tag="PW224_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2908278.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="PW224_001589"
     CDS             13749..14564
                     /locus_tag="PW224_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2908279.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="PW224_001590"
     CDS             14577..15224
                     /locus_tag="PW224_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2908280.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="PW224_001591"
     CDS             15240..15896
                     /locus_tag="PW224_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2908281.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="PW224_001592"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="PW224_001592"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL2908282.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="PW224_001593"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="PW224_001593"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL2908283.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="PW224_001594"
     CDS             18238..18756
                     /locus_tag="PW224_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2908284.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /locus_tag="PW224_001595"
     CDS             18863..19363
                     /locus_tag="PW224_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2908285.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19643..20563)
                     /locus_tag="PW224_001596"
     CDS             complement(19643..20563)
                     /locus_tag="PW224_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL2908286.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20728..21741
                     /locus_tag="PW224_001597"
     CDS             20728..21741
                     /locus_tag="PW224_001597"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL2908287.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="PW224_001598"
     CDS             complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="PW224_001598"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL2908288.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22826..23266
                     /gene="fabZ"
                     /locus_tag="PW224_001599"
     CDS             22826..23266
                     /gene="fabZ"
                     /locus_tag="PW224_001599"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2908289.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23381..24142)
                     /locus_tag="PW224_001600"
     CDS             complement(23381..24142)
                     /locus_tag="PW224_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2908290.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24331..25425
                     /locus_tag="PW224_001601"
     CDS             24331..25425
                     /locus_tag="PW224_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2908291.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25511..>26171)
                     /locus_tag="PW224_001602"
     CDS             complement(25511..>26171)
                     /locus_tag="PW224_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDL2908292.1"
                     /translation="VSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQR
                     SIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIE
                     TALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSN
                     GILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSL
                     I"
BASE COUNT         8651 a         4537 c         5510 g         7473 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc
    19501 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac
    19561 gtgtttagag cgtgggacaa aagtgttctt tacttttgtc ccacgctctt tttagtgaga
    19621 ttcgttgcat cgtttgtcca aattattttc cgacagcaat tggactgttc ggaatcgtga
    19681 aaccagaagc tgtcaattct ttgacatagg agctcaagaa ctcttctttt aaggcatatt
    19741 gttttccgtt caatgcgtag cagaccgtac gaatcgcaaa attgctgttt ccaagatcca
    19801 ctaatccaaa gatagaaggt tcagtttgga gctcttcttg gtatttttca gctaatgtac
    19861 gatttactcg atcaatgatc tcatagattt tgtcatatcc ttcttcagga acaatccgaa
    19921 tatctaaaag tacttgcata tttgcacgag agagattgct gatcgtggtg atgtttcgat
    19981 tcgggatgaa atggacagta ccatccgttg ctttcagctg aagggtacgg ataccaacag
    20041 atacgacggt tccttcaatc gttaagtttg ctagcttgat gtagtcccca acatccatct
    20101 gttgttccat gataataaaa aatccggtaa taacatcact catgaaccct tgggcaccaa
    20161 gaccgattgc cacacctgca attccggcac cagccaaaag tgatccgatc ggcacaccga
    20221 tagcagataa aattgcatag ataaaaaaga aaccaagggt ataatgaaaa atagtattta
    20281 aaagactatg taaagtcttt aagcggctct cgtttagaat ggctttttta gaatactgat
    20341 taaaagactt atcaatcaga tattttccca cacggtgaag aatccaaaaa aggacgatca
    20401 aaaaaatcag atataaactt ttttggataa aggcagccaa aatggcgtcc caatcaatcc
    20461 cgtcccaaaa ccgctgaaac gcatttactt tgctgacagc ttgatctgtc aatgtctcgc
    20521 cagaagagtc tgtcgtaccg gcagttgcta ataatgaaaa cataaaatcc tcttttctaa
    20581 aatttgattc tttctgtatt gtaccaagaa gttttcaaaa aaggaaatag caaaaataac
    20641 tataatgaag gaaaaaagct gaaaataaaa tgacaaataa tatggataat gataaaatgt
    20701 ggagaaaata acgaaggtgg taagtatatg caaatcgatt ggaacaatct aggtttttct
    20761 tatatcaaaa ctccttggcg ttttattgcc aaatggaaag acggacaatg ggaagaagga
    20821 gaattgacag aagataatta cctgacgatt catgaaggct ctccagcgct tcattatggt
    20881 cagcaatgtt ttgaaggtct aaaagcttat cgtcgaaaag acggcaaggt caatctcttt
    20941 cgacctgaac aaaatagtcg acggatgaat caaagtgcta gacgattgct gatgccaatg
    21001 gtccctgaaa agatgttcgt tgatgctgtc aagcaagtgg taaaagcaaa tgaagatttt
    21061 gtgccgccct atggaacagg cgctacatta tacttgcgcc ctttactgat tggggtcggt
    21121 gaaaacattg gtgtccatcc agctccagaa tatttatttg tcgtcttctg tacgccagta
    21181 ggagcgtatt tcaaaggtgg actaaaacca acgaatttta ttgtatctga ttacgatcgt
    21241 gctgctccta aggggactgg tgcagcaaaa gtcggcggaa attacgcagc gagtttactt
    21301 ccaggaacag aagcaaaaga actggattac tcagattgtg tttatcttga tccagccaca
    21361 catacgaaaa tcgaagaagt aggtgcagca aatttctttg gaattacaaa agacggaaaa
    21421 ttcatcactc cgcaatcgcc ctctatctta ccaagtatca cgaaatattc cttgttgcag
    21481 ttagctgaag aacgactagg actgcaagca attgaaggag atattttcat tgatgatcta
    21541 gatcaatttg cagaagctgg tgcatgtggt acagcggctg ttatttctcc aatcggtggg
    21601 atttatcaca ataacaatct tcatgtattt tatagtgaaa cggaagtagg accagtaaca
    21661 aaacggttgt atgatgaatt gacaggtatt caatttggag acatagaagc accagaaggc
    21721 tggattgaag tcgtggaata atttcaaagt aatgagttga aaaaaacgta aaaagaaaaa
    21781 atcaggaaga gactttaaaa agttttttcc tgatttttat atgttttact ttaaagaatg
    21841 caacaaaagt ttatgaaaga tggacacctt tgtcaacgta gataatatca ccgatgattc
    21901 cgctggaaag tgggctgatt aagaaagcac aagcattacc gacttcttcg atcgtcacac
    21961 cttgctgatc aggcgtacga gattcagata actggatcag tttttgatag tctttaacac
    22021 ctgtgacagc taatgttttg attgccccag ctgagattcc attgacccga attcctttag
    22081 gcgagaactc agctgccaaa taacggatag cagcttcaag agaagctttc gcaataccca
    22141 tcatgttata attcgggatt gcgcgttctg aacctagata agtcatgcta acgatcccgc
    22201 tgtttggtgc aagaaacggt tgtgcgtatt ttgatacagc aattaaagag tagctgctga
    22261 tatcttgagc taattgatag ccgtctctgg aaatatcaga cacatttccg gacaattctt
    22321 ctttgttggc gtaagcaact gcatggacaa ggccgtggat ctccccagca tattcaccga
    22381 tggcattcat tgctttttcg atgctttcgt cgctagaaac atcacattct acagtgaatg
    22441 cttcttcgcc gaccaatttg actaatgatt ttttcattcg ttcattttgg taagtataga
    22501 taatttctgc cccttgtttc aacattgctt cagcacaacc ccaagcaatg ctttttttgt
    22561 tagcgacgcc cataatgacg attttttttc cttctagaaa tgacataatg actctccttt
    22621 gtttctgata aaaatttgat agtcgtaaaa aaatagagaa taatagctaa aataacttct
    22681 tgatttctta attattagtc aaaatgtttt gattgtcaaa ctattttttc ttaaattaaa
    22741 aaaagattgc taaatattta ggaatatgat actttgatag tcaaagtaat gaaaatggca
    22801 attgcctgaa agaaggagtt taattatgtc ttacgtatta tctgctactg aagtaatgga
    22861 attgattcct aatcgttatc ctatttgtta tattgattat gtagatactt tagaaccagg
    22921 aaaaaagatc gttgcgacaa aaaatgtaac aatcaatgaa gattttttcc aaggtcactt
    22981 tcctgacaat ccagtgatgc cgggtgcgtt gattctagaa acactggcac aagcaggttc
    23041 gatacttatc ctaaaatcag aagagtttct aggaaaaaga gcttatatcg gtgggatcaa
    23101 taaggccaaa ttccgtcaaa aagttgttcc aggagatgtg ctgaaatgtc attttgaaat
    23161 caccaaaatg aagggatcag tcggtacagc gttatctgca gcatatgtag atgataaaaa
    23221 agtctgtgag tgcgagttca cgttcatcgt caaccaaaat gcttgactat aaaacctgat
    23281 atagcaaaac aaaaggaagc ttacttgctt tttatgtaaa aagaagacgc ctgaatccta
    23341 gcatacgttg tcatctatgc aggattcaga cgttattttt ttatttttgt tttgcttctt
    23401 gcagtatatt gattttatcg atttttactt cttcttttgg tttgtctgaa ctatctgttt
    23461 cgatatttgc aatagcatcc acgacatcca ttccttcaat cacttggccg aagacagtat
    23521 aatggccatc tagtgaagga taaccgccgt ttttataagc gtcgatgatt gcttgaggat
    23581 agtcgtcttt taaaagacca tcatgcatat catctgtatt ttgaacgata aagaactggc
    23641 tgccgttact gttaggatct tgtgagcgag ccatagataa cgcacctctt atattataaa
    23701 ggtggttgtt gatctctgtt tcaaatcctt ctccccaaat gctttcccct ccagtgccat
    23761 cgcctttagg atcgccacct tggatcatga aatctttgat gacgcgatgg aaagtcagtc
    23821 cgttgtagta accgtctttt gcatgtgtca taaagttttc aaccgctttt ggtgcgtgtt
    23881 cgggaaataa tttgattttg attgtgcctt tattcgtcac catttcgacc agatcttcat
    23941 tttcggcgac atctttcgtc aattgtggta attccaatgc gttcaaatcg acgctactgg
    24001 cagtcgtgct ttccgcagta gatgtacttt ttgaactttc tgtagacgaa ctagaagaag
    24061 tcgtgttgtt tgcagaacaa ccagcagcaa acagcaatgc tgctgctaaa ctaatactta
    24121 aaataacttt atttttcttc ataactgttc ctttccaatt tactattttg ttatctttaa
    24181 tgataacaaa atagagggaa aatacctact taaattattg gaatttatca tttattttaa
    24241 aagaaaaaga aaaggtgtag accatttact ttgtgtttgc tataatgtaa gtggatacaa
    24301 aaacgaaaag atgcaggagg aaatggtaat atgggaaaat taggaatctc gatttatcca
    24361 gaacggtcaa catttgaaaa ggacaaagcg tacttggatt tagctcataa gtatgggtat
    24421 aaacgggttt ttaccagttt gctgcagatc aatgatgata aagaaaaagt tctttcagaa
    24481 tttaaagaag tagttgatta cgcgaatagc ctaggcatgg aagtaatggt tgatatcaat
    24541 ccagcgttat ttgaacagtt ggagatttct tacgatgact tatctttttt ccataagatg
    24601 ggtgcctacg gggtccgctt agatatcgga ttcacaggag cagaagaagc aaaaatgacg
    24661 cgcaatcctt ttggtatcaa aatcgagatc aatatgagtt cgggcacgaa ttacgttgac
    24721 aacatcatga gttattcgcc aaacacggat aatttattag gttctcataa tttttatcct
    24781 catcgatact ctggtttagg atacgaacac tttgtttttt gttcagaaaa attcagaaaa
    24841 tataatttga atacgatggc attcgtcaat tcgcaatctg ctgagtttgg cccttggcct
    24901 acgcaagatg gcttgtgcac gctggaagac caccgagatc ttgaaattgc gacacaagtg
    24961 aagcatttaa tccttacagg attgattgat gatatttcta ttggaaatgc gtacgcaagt
    25021 gaagaagaat tgaaagaaat ggcagaagca tttaacgcgg attatccaac attgaaagta
    25081 gatacggaag aaggtattac agaaaacgaa cgaatctgct tgtttgataa ccttcatagt
    25141 tatcgagggg atcgctcaga atatatcctt cgttcaacga tgactcgtgt atactacaaa
    25201 gataaagatt tcccgcctca taatacgcga gatatgcatc atggggatgt actgattgac
    25261 aatgaagggt acggtcaata caaaggagaa acacaaatcg ccttgaagga tatgaaaaac
    25321 gacggacgtg tcaatgttgt aggaagaatc tccgatgacg aattattcct ccttgacttt
    25381 ttgaaaccat ggtcaagctt taaattaatt gaaaataaaa aatagataat tagttattgc
    25441 agaaataatc cgctagaaat agccagttat ttctgctttt ttattttgtt tttttatagg
    25501 ttatgatttg tcaaattaag ctagaataaa atgcttcttg tacatagctc aaaaatattt
    25561 cgattggtgt tctgtaattc aatgattttc ttggaatatg attacgttga ttgctgacac
    25621 tggaaataaa tgtctgattc acttttctaa aatccattga tttcggcagt ccattacgac
    25681 gcagaatccc gttagaattc tcgtttaatg ggcgttgaga aggtgttcca gggtccgcaa
    25741 aatatatatc aatatcatgt tggttactaa tggctttcca gttagaaaat tcttttccac
    25801 agtcaaacgt aatagattta aagaagtttt tagggaagcg agaaaaccat tgattaaggg
    25861 cagtttcaat atctaatgcc ttacggccgt tgggtttaat cgtgataatg actttagata
    25921 atctttcaac taaagtaatg acggcacttt tatgatgaat gccaacgatc gtatcacctt
    25981 caaggtgacc aaactcagaa ttgaaatcag gataattatc aggtcgatca tggattgagc
    26041 gctgatactg ttgttttccc cgtttttcct gatggccatt gggttttctt ttacctttca
    26101 tcggtagtgt gtcaatatca aatattcctt tagaaaataa acgataaagt gttctcatac
    26161 cgcatgaaac a
//
LOCUS       DANKMN010000041        26161 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000041 DANKMN010000000
VERSION     DANKMN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="PW224_001603"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="PW224_001603"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL2908293.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="PW224_001604"
     CDS             1286..2461
                     /locus_tag="PW224_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2908294.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="PW224_001605"
     CDS             2465..3106
                     /locus_tag="PW224_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908295.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="PW224_001606"
     CDS             3103..4020
                     /locus_tag="PW224_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2908296.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="PW224_001607"
     CDS             4017..4688
                     /locus_tag="PW224_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908297.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="PW224_001608"
     CDS             complement(5229..5717)
                     /locus_tag="PW224_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL2908298.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="PW224_001609"
     CDS             complement(5918..6184)
                     /locus_tag="PW224_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2908299.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="PW224_001610"
     CDS             complement(6184..6447)
                     /locus_tag="PW224_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2908300.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6923..7108
                     /locus_tag="PW224_001611"
     CDS             6923..7108
                     /locus_tag="PW224_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2908301.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7250..9073)
                     /locus_tag="PW224_001612"
     CDS             complement(7250..9073)
                     /locus_tag="PW224_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL2908302.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="PW224_001613"
     CDS             complement(9671..12376)
                     /locus_tag="PW224_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL2908303.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="PW224_001614"
     CDS             complement(12682..13629)
                     /locus_tag="PW224_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL2908304.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="PW224_001615"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="PW224_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2908305.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="PW224_001616"
     CDS             complement(14264..14626)
                     /locus_tag="PW224_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2908306.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /locus_tag="PW224_001617"
     CDS             complement(14637..15758)
                     /locus_tag="PW224_001617"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL2908307.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /locus_tag="PW224_001618"
     CDS             complement(15774..16124)
                     /locus_tag="PW224_001618"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL2908308.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="PW224_001619"
     CDS             complement(16254..17765)
                     /locus_tag="PW224_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2908309.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /locus_tag="PW224_001620"
     CDS             complement(18087..19439)
                     /locus_tag="PW224_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL2908310.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="PW224_001621"
     CDS             complement(19592..20668)
                     /locus_tag="PW224_001621"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL2908311.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="PW224_001622"
     CDS             20864..22609
                     /locus_tag="PW224_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL2908312.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="PW224_001623"
     CDS             complement(22889..23326)
                     /locus_tag="PW224_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL2908313.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="PW224_001624"
     CDS             complement(23310..24683)
                     /locus_tag="PW224_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908314.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="PW224_001625"
     CDS             complement(25005..25493)
                     /locus_tag="PW224_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2908315.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="PW224_001626"
     CDS             complement(25515..26117)
                     /locus_tag="PW224_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2908316.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7503 a         5561 c         4838 g         8259 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
    26161 t
//
LOCUS       DANKMN010000042        25897 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000042 DANKMN010000000
VERSION     DANKMN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(178..585)
                     /locus_tag="PW224_001627"
     CDS             complement(178..585)
                     /locus_tag="PW224_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2908317.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(580..1784)
                     /locus_tag="PW224_001628"
                     /pseudo
     CDS             complement(580..1784)
                     /locus_tag="PW224_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            1910..2128
                     /locus_tag="PW224_001629"
     CDS             1910..2128
                     /locus_tag="PW224_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908318.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2149..2784
                     /locus_tag="PW224_001630"
     CDS             2149..2784
                     /locus_tag="PW224_001630"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2908319.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2977..3216)
                     /locus_tag="PW224_001631"
     CDS             complement(2977..3216)
                     /locus_tag="PW224_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908320.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3240..4763)
                     /locus_tag="PW224_001632"
     CDS             complement(3240..4763)
                     /locus_tag="PW224_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2908321.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4781..4903)
                     /locus_tag="PW224_001633"
     CDS             complement(4781..4903)
                     /locus_tag="PW224_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2908322.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4933..5916)
                     /locus_tag="PW224_001634"
     CDS             complement(4933..5916)
                     /locus_tag="PW224_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL2908323.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5940..6089)
                     /gene="rpmG"
                     /locus_tag="PW224_001635"
     CDS             complement(5940..6089)
                     /gene="rpmG"
                     /locus_tag="PW224_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2908324.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6110..6487)
                     /locus_tag="PW224_001636"
     CDS             complement(6110..6487)
                     /locus_tag="PW224_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908325.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6519..6698)
                     /gene="rpmF"
                     /locus_tag="PW224_001637"
     CDS             complement(6519..6698)
                     /gene="rpmF"
                     /locus_tag="PW224_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2908326.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6713..6982)
                     /gene="rpsN"
                     /locus_tag="PW224_001638"
     CDS             complement(6713..6982)
                     /gene="rpsN"
                     /locus_tag="PW224_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2908327.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7505..9271)
                     /locus_tag="PW224_001639"
     CDS             complement(7505..9271)
                     /locus_tag="PW224_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2908328.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9231..10985)
                     /locus_tag="PW224_001640"
     CDS             complement(9231..10985)
                     /locus_tag="PW224_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2908329.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11095..11664)
                     /locus_tag="PW224_001641"
     CDS             complement(11095..11664)
                     /locus_tag="PW224_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL2908330.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(11682..13097)
                     /locus_tag="PW224_001642"
     CDS             complement(11682..13097)
                     /locus_tag="PW224_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2908331.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(13108..13776)
                     /locus_tag="PW224_001643"
     CDS             complement(13108..13776)
                     /locus_tag="PW224_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2908332.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(13907..14491)
                     /locus_tag="PW224_001644"
     CDS             complement(13907..14491)
                     /locus_tag="PW224_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2908333.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(14826..15056)
                     /locus_tag="PW224_001645"
     CDS             complement(14826..15056)
                     /locus_tag="PW224_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL2908334.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(15071..16018)
                     /locus_tag="PW224_001646"
     CDS             complement(15071..16018)
                     /locus_tag="PW224_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2908335.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16018..16860)
                     /locus_tag="PW224_001647"
     CDS             complement(16018..16860)
                     /locus_tag="PW224_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2908336.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(16993..17718)
                     /locus_tag="PW224_001648"
     CDS             complement(16993..17718)
                     /locus_tag="PW224_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2908337.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(18179..18493)
                     /locus_tag="PW224_001649"
     CDS             complement(18179..18493)
                     /locus_tag="PW224_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2908338.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(18509..20104)
                     /locus_tag="PW224_001650"
     CDS             complement(18509..20104)
                     /locus_tag="PW224_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL2908339.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(20127..21017)
                     /gene="iolE"
                     /locus_tag="PW224_001651"
     CDS             complement(20127..21017)
                     /gene="iolE"
                     /locus_tag="PW224_001651"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL2908340.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(21034..22059)
                     /gene="iolG"
                     /locus_tag="PW224_001652"
     CDS             complement(21034..22059)
                     /gene="iolG"
                     /locus_tag="PW224_001652"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL2908341.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(22083..23090)
                     /locus_tag="PW224_001653"
     CDS             complement(22083..23090)
                     /locus_tag="PW224_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2908342.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(23203..24363)
                     /locus_tag="PW224_001654"
     CDS             complement(23203..24363)
                     /locus_tag="PW224_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDL2908343.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(24439..25893)
                     /locus_tag="PW224_001655"
     CDS             complement(24439..25893)
                     /locus_tag="PW224_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL2908344.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         7945 a         5295 c         3975 g         8682 t
ORIGIN      
        1 aataagtaat ctaaccgact gttaactaaa ccattgcagg tcccgttaaa gatacttgtg
       61 cctgttgtca ccgcttcttt ttcttgtcca caagcgctta acacacttaa taaagcccct
      121 gctattatta ataataatat ctttttctta ttaaaaacct cctcatctat ttccagatta
      181 gtaaatgtag taatctttgc tttctttttt ctcaatacta ataactaact tatggggtaa
      241 acaaatactc gtttgaccgg gttttgagat ccatccagtt ctaaccgcga tttgatcagg
      301 actattatct tctttaactc gaattcttcc atctttaatc tcaacaatat tatattgtcc
      361 cttcgcagga tgataattca cgagtttatg attgatctta tccaaatcga agcgatccat
      421 ttccttgcca tcaatttgaa cgatggcata tttgatagca tcttcagttg tttcattctc
      481 cttttctata gagaatacaa ataagggaat aaaagaggga ataatcaaaa tgccaataat
      541 tatgacatcg cctatcttga caccagcttt tcttaaaaat ttcattaacg atatctggct
      601 aaacatatta gaatttttcc atggaaatgg tgaaattttt cggaaattat ctgcattaag
      661 agaatcagtt aaacaaccct caagttgttc cttaatacat tgttgatcca tcttcatacc
      721 aataaaaact agtttattaa tgcgttcacc aaacaacgga tgccaggctt ccttcaattc
      781 gggttttttt gtataatcat ttgacactca attttaggta aactagctac ccaataattt
      841 aatgattcaa tacttttatg gctacccgat gtttcaaaga tcgtcgaaaa atcactatgt
      901 tgggcaaacc aaagaaaccc ttttgctctt gtaatattgc caggaaaatt tttatccgta
      961 agatgggcaa atttttgtgc gtcaaggagt aaccgatttc gatagataaa acttgagata
     1021 ccatattctt ctgtttcagg aatatgattt tgatagccaa gctctaactc tttgaaccag
     1081 cccattgaat aggaagtctc atctatatta aaaagatttg tatttagaat tttcgataag
     1141 tctacttcac ctttaattgt cctaattatt ttggcattgg actgtaactt agcaattaga
     1201 ccgttaattt tgtaaatcgt cttggttgac actaagtcac tattatttaa aagtaatatg
     1261 ttacaacact cgaattgatc aattaacaag ccacgaatat cctgttcttc ttcaactaca
     1321 ttaccttcag cttttctttc ctgaatcttt tcacccgttt cgaaattatc ccaaaaacgt
     1381 tttacatcca ctactataat aatggcacca atctctaatc gttgggtcaa atcaatgtct
     1441 aaagggtttt tagctaatat aattgcttga ataattggaa ttggtttgct gattcctgaa
     1501 gcttcaataa caatttgatt caagtcagaa aattgcacta attgatctag ttcaacaatc
     1561 aaatcttcgc gtaatgtaca acaaatgcat ccattcggca tcgaaacaag tttttctcca
     1621 gttcttttaa aacctttctt gtcgactaat accccatcaa catttacttc ccctatgtca
     1681 tttacaatga cagcaatttc ctttccgtga tcaccgtaca gaatatgatt tagaagtatc
     1741 gtttttcctg aaccaagata gccagttaag attgtaatag gtattttttc atagggaacc
     1801 tctttctata ccttgaattt acactttaag tgcaacacat aaaaatgagt cataatcgat
     1861 atccaactag tataagtttg tcgagaacac tcctagaagg aaaataatta tgagccagta
     1921 ttgtcatctt actatactca aacgagaaag aatctttctg cttcacgaag aaggattttc
     1981 tattagtata attggcaacg acattcagca tagcccgttt actattttac atgaactgag
     2041 aagaaacaag aaaaaaacta atcaccttcc aaagcggaaa aaaattatgc ttatcgaaga
     2101 aaaaaatgcg gacgttatca cattctaaat gatccgtgag tttggtcaat tgttcgtcat
     2161 ttatttgtcg attttcagtg gtcgccagag aaaatctcga atcgtttgaa gcatgaacat
     2221 atggactttt caattagttt tatcacgatc taccgtgcaa tttgcaatga tcttttcgat
     2281 acagaacccc tctcacacgg caataaaggc ctgatccgtt cattgcacca tcgaggaaaa
     2341 acaagacata caaaaaatca tgtcgagtct cgtgggaaga tttgccttac tcattcgatc
     2401 catggccatc caaaatccgc agatgataga agtgattttg gtcattggaa agccgatatt
     2461 gtagctggca aaactagtgg tacttgttta gtgactttga cagacagaaa atcatgcttc
     2521 cttttagcag gaaagaaggt ccccagaaag aaagccgctt atgtgagcga gaaaatgatt
     2581 gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa
     2641 gaattctcca atcatttcgc agttacagaa gtattgaata atatgccctt ttattttcca
     2701 gatcctcatg cccttggcaa caatgaacaa attaaaatac gaatagactc attcgagaat
     2761 acttgccaaa atcgaaagag atagctacgg tagataactc aacgataaat cagatgattg
     2821 aaaaactgaa taacagacct agaaagtgtt tagattggag aacaccttat gaagtcttct
     2881 ttgataaagt gttgcgcttg atttgacaat tcaagaaata ataaaaagca gatcaaatcc
     2941 aaataaggga aaaatgatct actctggtat tatcaattaa ttggccaagc tattatgccg
     3001 ttttgctaaa cgtctacttc ttccagtatt gaccttgata aaacaacaat caggatcccg
     3061 ttttaatact ctatgaactg tgtcaatact aacatgtaaa gttgcagcaa tatcagctaa
     3121 tgtactccca tttaatctca tttgctttgc aaattgataa cgccgataag catcaatatt
     3181 cttggaatcc atagacaata atacttttct taccatgtat ttcctccaaa gattggattt
     3241 taatgggcca tcatttcttg agcaatctca tgctcagata atgaagctgg atagaaagta
     3301 ggccaattat gcatttcatt ataaagtttt tcttgacttt ctccaccaaa gaaaatatgg
     3361 aaatgctctg ccttacttgg tgcaattcca tgatcgctaa attgaatata tttaaaacga
     3421 ccagcacctg catcatcagt ttcaaaatta aaacgaacac cacggtttcc tttttcataa
     3481 ttcaaaattt tgtatccaac atatttgtat gtgtattgat gatgttcacc gttgactaca
     3541 aaatcaatgg tgttgtcttt aatgttgata gtatcaatat ctgtcttata gccagtagta
     3601 taataatctt tgtactcggc agctgtcatg tctttattga gttttgcttt gtaatcgaaa
     3661 acttcatcca aaattccatc ttttaacaac ggataaacag attgccattc gcctgtataa
     3721 tcactcaatt cgcgatcctt cacatcggca tctttaaagt acccattagc aactgtcttt
     3781 tcatccttcg cttctttttc tggaactacc tcttcaccag caaccatagt tgttttctct
     3841 aacgctttta gattgtcttc cattactgaa acataatctt caccattatc catttgttct
     3901 tttgtaagac tttcaagagg attcaatact tccaatttta tcccagcttc attggccaaa
     3961 gtttttgcga ttttatcatt agcattcttt tcaaagtaaa tataattgat cttatttttt
     4021 ttaacatact cttttaattc agctaatcga ccagaagatg gctcttcttc aggacttagt
     4081 ccagcaatag gaacttgtat caatccataa tccaaagcta aatatccaaa ggctgcatgt
     4141 tgtgtcacaa aacttttttg tttcgcttct ttaagactag tggtatagtc agcatccaac
     4201 cgtttcagtt tagtcaaata cttttcagca tttgtctcaa atacttttgc ttttttcgga
     4261 tacaatttaa ctaactgatc cttgatattt gaaacttcct taattgccat tttgggtgaa
     4321 acccacgtat gaggatctag ttcatgatgg tgatcttctt ctccgtgatc atgatcttct
     4381 tcttcactac ctggcaataa aatcatgtct tttgttcctt ctactacatt aggcccagct
     4441 ttcttccaac tttctttagc ttttggtacc catgattcca tgttttcgtt atgatataca
     4501 aaaacatcag catcatgagc ttttgccata tccttagctg agggctcata gtcatgaggt
     4561 tctgatcccg ctggaaccat taaatccacg ttcgcttcat cgccgacgat gtttttggtg
     4621 aaatcataca ttggatagaa tgttgttaca atttctaatt ttccatcttt atcattcgat
     4681 acttttgcat cattagtgct ggagcaagct cccaataatc ctagagctgc tatcccaata
     4741 agtcctacac tgattttctt cattcttttt ccttccttaa ttatgctaat ttcttcattt
     4801 tattttgttt atgttctttg ataattttta atgccctttt tctagttttg atattcgggc
     4861 ttttaagtcg tcgatatgca ctagatatat ccgttttctc cattagaaat tacccctaat
     4921 caatacttat cattatttgc tttccgccag taatttatca aaactctgtt tgatcctttc
     4981 ttcatccaac atttttccaa ttagaataat agtggtttgg tctttttgtc gagtggtgta
     5041 ttgaaaatta acctgttcgt ttaccccttg tatggcaact ataaaatctt gatcttgcaa
     5101 agcaatcaat cctttaaaac gatacaggct cccttggttg gttaaaatca gccggtcaat
     5161 taattggaat aaaaaatctt tttttatgtc ttgatctgaa gttaagagaa ttgatttaaa
     5221 ctcatgttct tgatgatgtt gatgatagtg tttgccatta tagtgatttt ctgatgaatc
     5281 actttcaaaa actaagggat gattgaattt ttccaaatta taaaaatcct ttttttgaat
     5341 cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg ttaatacttt tgatttctct
     5401 ctgtaatttt tttagtgcag ataaattcaa gttttgtttt ctagacacaa aaatgcgatc
     5461 tgctatgaca agttgcttaa gggcttgtgg ttctctttca ataagttcaa aactgtcact
     5521 atcaataaca gtcaataaac tatcgatata tactcttcct cttagttgag gcgctgttaa
     5581 aagtgtttga atgattggtt ggggatctgc tatacctgta gtttcaatga tgatttgtga
     5641 aattggttgt tgatgttctt ctattgtttc cagtaaagaa atcaatattg ctaataaatc
     5701 actgcgaaga ctgcaacaca tacatccatt atttagttgt aacacccgtt cctcgctatg
     5761 aactaataat ttgtgatcaa tcccaacttc accaaactca ttttcaatta taataatttc
     5821 ttccctgggg tagggtgaga cttgcaaggc ttgattgatt agcgttgttt ttccagcccc
     5881 taaaaaccca gaaacgattg taactggtat gcccattttg tcaccttttt accacttagc
     5941 tatttcactt ctttgaaaat tgcccgtctg cgcaattttg gcgaatactt ttttaattct
     6001 aaccgttcag ggttattccg tttatttttg ctggtgagat acagacgttc tccagtttcg
     6061 acacattcca aaataatatt ttgacgcata aatcttcctc cttcaatact tatcgatctt
     6121 caagatatgc ttgaatttgt tgtgcatctt caaaagttaa ctcgccagat tccaattgtg
     6181 aaactaaatt ttctttcata tcaacaggta tctcccaata accatcaata cattgaaata
     6241 actcctcgtc atactcttca aaaattggat agattcttaa aaaatcatca aatgataagc
     6301 cttggtcctc atagtaatct ttgactaatc cattttcaat tgcctgttgg gtcggtaaac
     6361 ccgtttcagt aataattcct ctctctacca tcgaacgata aaaagtgcat ttatctagcg
     6421 attgctgggt cgtttcaaga taatacttta atccttcttc aactaaaggt gggtaaataa
     6481 tttccatttt gattccctcc taaaagttat atattaaact attttgaagc tcctttaatt
     6541 actttctttc cattatagta cccttttaaa gatacatgat ggctacgtct ataatcacca
     6601 ttcgcttcgt caaatgaaat ttctggtttt actacagttt gatgagttct tcttaaccgt
     6661 ttcttcgcct tagatgtttt tcttgctggt actgccatga tatttcctcc ttttaccaac
     6721 ttgctttttt tactccagga atcaaccctt gatgggcaag ttctctaaac ttaatacggg
     6781 acataccgaa ctttcgcata taccctcttg gacgaccatc cgtctgatca cgaagcctca
     6841 gacgattcgg atttgaatct cttggcaatt tagcgagagc ggtgcgatct cctgcggctt
     6901 ttaattcctg acgtttatct gcgtactttt caatcatcgc catttgtttc tttgctttag
     6961 caattttcga cttttttgcc atataccttt atactccttt tattttgatt tagacatctc
     7021 aaatcgtaat cgttacgttt tgacactgat actttaccaa caagagttgc tcttgtcaac
     7081 aataaataac atgaaataaa actcatatga atagataact atttgaaaac gaatttatcg
     7141 gtacatagag ataatcaaat catttaaaag aatatgcagt ttaagctctg aaaaataaga
     7201 attcactttt attagtccaa tcaaacacaa ataccgtttt aagtagcttt tcctctactt
     7261 aaaacggcaa tatgaaagaa actataactt tttattcaat cttttatgtt taactggttt
     7321 atcgccaatt ttttaaaact ttgattattc cttatattat aagaatagac attaagtgaa
     7381 ctaattcaat catactgatt ctttcgtgta ttcaaaatta gatgctgttt aataaaatta
     7441 ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt taaagcctat
     7501 ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct
     7561 ttatgagatt tttgtatatt tctgaccttc gatacaactc actatgagta ccttgatcaa
     7621 ctatttttcc ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat
     7681 gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt
     7741 tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt
     7801 tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt
     7861 tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag
     7921 ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat
     7981 tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa
     8041 acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata cagccttcat
     8101 cataatcata cagtctagac actaatttca agatactagt tttcccggaa ccactagcac
     8161 ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg
     8221 tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac
     8281 tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt attttaggag
     8341 ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta
     8401 ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt
     8461 gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca
     8521 tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt
     8581 tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt
     8641 ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg
     8701 ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa
     8761 taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa
     8821 acagagccat accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa
     8881 cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg
     8941 gtaaatttga caaatcgtct gcaatttgca ttcttaaatg cgcactttct ttataagtac
     9001 tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacga
     9061 acgatactgt aaaataaagc caaggcggct ttacattttc taaaactaat tcgtctatca
     9121 gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaag ctagaaatag
     9181 atgctttaac caaatcttct gcgccctgat tagttaaagc gaatttgttt ttataaaaac
     9241 ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac
     9301 acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac
     9361 aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat cataataact
     9421 gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca
     9481 atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca
     9541 atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta
     9601 tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca
     9661 aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga
     9721 ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa
     9781 tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct
     9841 gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt
     9901 cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt gtatgaaaag
     9961 ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc atattttagt
    10021 tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata tcttgcttca
    10081 aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat tccacttaaa
    10141 aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga agttataaaa
    10201 aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg aaaaagaaca
    10261 aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc gttaattgct
    10321 ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac ttgaattcct
    10381 cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata tttttgaata
    10441 aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa catggcgatt
    10501 agaaccaatc caattttaaa attaatgaaa aagcctaagg caactgataa aacaggtgtt
    10561 actatggctt gagcactatc tggaatcaag tgagcaattg ccatatgtgt tttagccgcg
    10621 ttgtcatcaa ttgtctttcg aatatatccc gaagaattta aatcaaaaaa acgaaaactt
    10681 gcttctgtta aaccgctaat tccctttttt ctcaaattag tctctaagcg aaacccaagg
    10741 atatgagaaa ataagccaga aaaaatataa aataaagctc ctaaagttaa ccaagcaaca
    10801 gtttgaaccg cataactctt agctaaatat tcatctccta caataattag agacttaaga
    10861 aatttgtaaa tactgtaata tccatagact gttaatacga cagaaaaatc agaaaataaa
    10921 gtggctaacc atccgtagta tttcatttta gggacataac taaataattt tttgtatact
    10981 ttcatttttt cctcccccgc attcaatttc ttctcttatt cgtttacaac tgttaatgct
    11041 ttccgtaata gcgtaaatta gagatgtaca aacagataga aggtaaacta cgttctattt
    11101 tttgtatttt tttaagactt ttcttccaat caatacacca atcatagata aacttgctac
    11161 aactaacact gaaatgattc cccatatacc taccgcatag tggatcattg catccattgt
    11221 tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa agaaccatat
    11281 aggaatccac tgtccggcag cactgcctaa ttgcattaga gcccaagacg caacttgagg
    11341 aattgctcga tcatatccaa atacagatag aataatatca gctaagatcg ccatactaat
    11401 cgaactgata aaccaaggta agtacgcccc gcccattaac gtaaaaaata acaataaaac
    11461 aattgtgtaa atagtaaaaa ttccttttct agggtagtta tagctaatat aaacaaatat
    11521 gattccagat aataatccaa aaatccctgg ggatatgctg tgaccaaacg ccccaccaat
    11581 actactaacc attattgaaa aaaagtaaat tactagggaa ataactgtag ctaaaccaat
    11641 aaaaatataa tcttttgttt tcatatatat tctcctcaat tctatttcgt tatgtcatta
    11701 aaaatctgtt ctatcaaagt tgaatcttct actctaagtt ttatatcttt ttttattcca
    11761 ccatttttta aataaataat tctattgcat gttttataaa gaaattccgg atcatgagtg
    11821 gctataacta ctgcaccctt ttcttttaag tctttaataa ggtcagcaat cctttccata
    11881 ttaatataat ccaacccact tgtaggttca tcaaatagaa ataaattagt ttttgacaat
    11941 gaagctatag aaaccaataa tctttgtttt tctccaccgg ataaatcaaa cggtaattga
    12001 tctttcattt tataaagttc catttttttt aaaatttttt cttgctgatc tttactaact
    12061 atttcattcc ccagggctaa ctcgctcctc actgattctg tgaaaaattg gtaatccatt
    12121 tcttgcatta ctaaaaacgg cgatgacaaa ttttttttat tttcgattat ctttcctttg
    12181 gtagggcgaa taatcttcat gagtattctt agtaaggttg ttttccccac accgtttttc
    12241 cctaccaaac caataacatc tccaactttg acatcaaaat ttatgtcttt tagaatattt
    12301 tcataagtaa tttcttcaac ttttaataca tctgcactta ccttttcgat atagggaact
    12361 tcaatcgaac tattaaaaat gtcaaatttc cttaatgcgt tattgttttc agtttttaac
    12421 aactgaatag ttgtagtttt tacagttcta tttttaacaa atagaacttg atcaattaat
    12481 tctttcaaat agtaaaaacg atgttcagct ataacaatag taaatccttt attttttaaa
    12541 tcacaaagta agtcagcaag tttcttagtc gctttgaagt ccaaatttga agacggttca
    12601 tccaatataa ttaatttaga gtttagcgat aaactacatc caatagaaat tttctgccgt
    12661 tcaccacttg atagcgaaaa aatgttttta tctaatatat ttgaaagatc taataattca
    12721 gctaattcat tgattctttt atccatatgc tcttttgtaa atccaaagtt ttccatcgaa
    12781 aatactaact cactcgttgt atttgttgta aagaattgtc ctctaggatc ttgacttacg
    12841 tagccaatat tttttgatat actttcttgt gttaaggatt gtagcgagtt attttcaaat
    12901 aaaatttccc caaccaattt tccgtcataa agtcttggaa taagtccatt cattaacttt
    12961 aatagtgtgg atttcccact cccagaagaa ccagtaatca atgtaagact accttttttt
    13021 attgaaaaat taaggtcaac aagcgcttgt tccttaacat ccttatatga aaaagaaaca
    13081 ttgttaaatt caaacaattc tttcacctca aatcaaaata cttaagaaaa aaatagtaac
    13141 ggaaaacagg atgatatagt ctaataagct catgtttgtt ttatagtaag aacttctttt
    13201 cacaggagat tctatcccct ttgttattgc attagcggtc atttcttcag caatcatagt
    13261 tgcacgtata agaacgggaa ctaacagaaa ttccaaatat tgcagaggct gaaaaaaaga
    13321 caagcgaatg tttctcattt ttaaagattc tttaatcatt cttatttcag aacgataagt
    13381 cggaaagaat ctgatcacta ctgtaatact taacattatt tttttcggta aatgtatttt
    13441 ttctaaagag gataataatt cattgctagt atagacacta accaacaacc atgagatcat
    13501 tacgaccggc aacattcgaa atacaatata taaggatgtc ccaaaaaata tagtaaaaaa
    13561 ctctatcttg atagcatcaa taattttcat tactaaaaac agaactgaat aaatcaagat
    13621 gaaataaata catgtcttta ttttacgaaa catcaatact aaaactgtag caataatgaa
    13681 acaccccact tgaaatatat ttccctttcc taaaaaaata atgaccggaa aaaaaatatt
    13741 taaagtaatc aaggttaacg gattgatttt tgacactctt tcacctccaa aaaataagat
    13801 cgcactgtta tcttaagctg ataaaaatgc accgttattc agataacagt gcgatcttat
    13861 caaaaagcat aactgagaac aattctcaat gcaagctttt ttttaattaa ctcagtaatt
    13921 caatccaccc aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt
    13981 ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt
    14041 ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat
    14101 ctagacaaca gactgtcttt tttgtcacaa atgatgtaat tttttcaaca atatcttgat
    14161 atttggttcc agctgattgg aataaaacca aatcgaatat acgtttattc tcaaaaatat
    14221 aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct atatctatat
    14281 tttctaagtt taagaggtat ttggtaaact cttcattata catattaata agcgcattaa
    14341 tatgcggctg aataatttct tcaaataacg cttccttaga agtaaaatgt ttataaaaag
    14401 cacccgtagt tacgcctgcg gtcaagcaca gttgccttat cttagtttgc tgaaatccta
    14461 ctttttcaaa atggcttagt ccactttcta ttaatttttg gtgagttgta atataatctc
    14521 tggtttctat gggtacttcc tcctaataag atgaattaac tatattatct tacacaggac
    14581 ttgaagtttc aatcgttaac gtctattact gaactcattc cttagagcta aactaatgag
    14641 aaagagcaaa ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat
    14701 cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg
    14761 ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa aataaagcct
    14821 tttacttaaa accttttttt gtaatcgtca ataaattttt tccctttatc agttaattga
    14881 aatcctatat ctaaatagtc ctcatatcta agtttagtca gcatctcact tacagatgct
    14941 ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat
    15001 aattcctgaa ttgctagtaa ataaattagt atagtagttg ttttaatttg taccatacct
    15061 accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg
    15121 catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat
    15181 agataggaat attggtatct tttgatactg ttttcattgg tctatcgtcc acactgcttt
    15241 ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat
    15301 ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg
    15361 ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa
    15421 ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca atatacttat
    15481 ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa
    15541 tatttttagc gtaaataata ccgttttcta aatttaacca agcatgggga tcttccttcc
    15601 ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt
    15661 ctttgttttc ctctttgttc gcatttttta ctaattttgt aaaccaagca tttcctccag
    15721 tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt
    15781 caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa
    15841 ttttatcttt tgctatattt tcagtaatat ctgctaaaat cgaattagta actactacct
    15901 ttaatttttc gttcgaagcc actgtatctt tgtctttact atttgttgaa catgccccga
    15961 atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca
    16021 taactttctc tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac
    16081 aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc
    16141 aatgaacaaa cctagcaatg atgagcacgc tccgactgtc gccgaaataa atatcatagt
    16201 ttttaaactg tttgcatata aatatgctgt agcagctggt gtaattaaca ttgcaacaat
    16261 taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata gaatcattaa
    16321 tagataatga taaaaactga cattcatccc cattgctttt gccattaatg gatcaaaaga
    16381 agtgattaat aattctttaa aaaatagaac aactgtaacc aacactaaaa cagaaacgcc
    16441 aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata agatatgaaa
    16501 taaatccgtt gagctatttg caactcctat aaggataact cctaatgcta aaaaagaact
    16561 gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat
    16621 aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat
    16681 ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat
    16741 tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt
    16801 aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat
    16861 attattcccc ttgtatatta gagttagggt tttaaccttc cggttttgat tggataaccc
    16921 tatgaaccat cgttatttta atttggtatt aactcatttc ttttgagata atatatttat
    16981 tggattgaga ggctaagccg ttcccccctc tccaataaat atagtatctc cataggcttt
    17041 ttttaaattc tcttcattaa atacagattc tacacttccg tgagcaatta gatttcgatt
    17101 caaaagaata atattatcaa aatacttctt taccttgctt aaatcatgat gcacaattag
    17161 cactgttttt ccctgatgtt tcaattcttt taacgtatcc ataattattc gttcactaac
    17221 taagtctatc cctacaaatg gttcatctaa aaatatgaaa tccgcatttt gagcaagaca
    17281 tcttgctaaa agtacacgtt gaaattgtcc tccagataat tctcctattt gatgattaga
    17341 atattttccc atatcgacct tttccaaagc ctttgaaact ctttgccatt ctgcattttt
    17401 aattctttga aaaactttca ttccggcata tgttcccata gatacacatt cttttacttt
    17461 tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct tcaccagttt
    17521 tttattgata tcttcattat taatggagac ctctccctga tgagggataa tatttaacat
    17581 tgctttaagc aatgtagatt ttccagcacc atttggacca ataatacctg ttaaagcacc
    17641 ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac aaacattaag
    17701 ctttttcact tctaacataa ctttctcctt ctaaatgata atgattatca cgatcatatg
    17761 gtatacaaaa attatatttt agtcaaccct aaattttata atttagattt ttttctgttg
    17821 ttgaagatta ggatgtcaaa attatttgaa actactcgta tagtaaatag taagtaattt
    17881 tcaaaacacg aagaaaaaca aaaagaaaaa aggaaacgcc gaagcatttc ctttcagtga
    17941 tttaatgaga acaacacgat ttgacttaga gtctcaaaaa gtcgaatagg tacactcatt
    18001 taagtgtctc cattcgactt tttggggatg agctggctat tgaggtccgc ttaccctatt
    18061 gaataaatac aagattgggt atttctaata agtccgccct tcatattggg gtgatcgggg
    18121 cgaacatgcc atcttttcat ataccacctc ctcgacactc aataatcaaa gattctcgtt
    18181 aatcttcttc gatgcttgca atgatgtttt caatattcat ataacgaaaa tcttcttgtt
    18241 tgatataaac gatctgagcg gtacattttt cctttttgat gatttctttt actttttcct
    18301 cagaacgtgc gacctgcggc gttaaaagta aaacgtcata gtctttataa ttcagttttt
    18361 cttcaacgtt tcggatcgtt gcataatcta tctcatacga tagatttttg gctctgatcg
    18421 tttcaagcat ttttgatacg agcagcgacg cactgattcc ggtctcgcaa ataatcaata
    18481 gatccttatg cataataccc tcctgttttt agcctgcaaa gccaattggt gaaaatgcca
    18541 cataagttaa aataacaatg actaccaatg ctatagcagt caccttggtg tatttccaag
    18601 gctcgatatc gacctttgta tcgaatgagt tcaacacaaa ttctccatca ggtttcaatt
    18661 tggaaaccac catcaaaatg ataatgtcca gaacgaataa tgccgtcaaa acataaagga
    18721 aatggatatt ttggaaaagc atttttgata gtgcatacaa gacgatatgc acggcaatcg
    18781 tcgtttttgc cccaaaagca gttgcttttt ttgaataaaa ggcaaagaga atgatcacga
    18841 gcagcggcat gttgtatagt ccattgaatt cttgaacgaa ctgatacaat ccagccggtg
    18901 caaaagaaat gaatggcgct acgataatgg agataatccc cacaacgacc gttactgttt
    18961 taccagcacg agcgatttgc ttatcatttg ctttcttatt gataagcggt ttataaaaat
    19021 ccagtgaaaa caaggtcgat gtcgcgttca aagctcccgc aaatgatgat aagatcgcgc
    19081 caaagataac agcggcgaaa ataccaaaga tcattttagg caatacttca ctgactaaaa
    19141 aaggatacgc gttatctgga tttgacatta aactatcgcc aaaaatattg cgagcgacga
    19201 tccctggaaa gacaaggaat aacgcaccta agattttgaa gacgccgact aggaaagcgc
    19261 ccttttgtgc ctcagctaaa ttcttgccgg acaatgcttt ttggacgatc atttgattgg
    19321 tacaccagaa atacagattg ttaaacagca tccctgtaaa tagtgtcggc catggaacaa
    19381 cagccgagtc gatcgatccc acagagttaa gcagccatgg cgtgtttacc acaacgtgtt
    19441 cgatcccgcc aacaaagctg ccgtcaccaa gcttcattaa gcctaaaatt gggaccagca
    19501 gaccacaagc cagcagaccg actccataaa tggagtcact attggcactt aacgacaagc
    19561 cgccgatcag cagatagagg atgccgacaa taccaattgc catcgctaca attatgatcg
    19621 cgacgatcgg ctggacgccc agcagctcat caatatggaa gatcttattg aacaccagcg
    19681 atccagaata aagaaccacg ggtaagaaag aagtcatata ggtcacaata aataagatcg
    19741 aaataattcg tttggtaacc gtatcataac gaatctcgat gaaatctgag attgtattga
    19801 tgccgtattt aaaatatttt ggcaagaata tcacagccaa agctacgatc gcaatcgtag
    19861 cagttacttc ccaggccatt acttccatcc ctgcatgata actttgacca ttttgtccga
    19921 ctatttgttc ggtggacagg ttcgtcatga tgatcgtgcc ggcgattggc aaagcagtta
    19981 agctccgccc tcccatgaag aacccttctg atccagaagt atcgacactt ctggatctag
    20041 aataggcaaa taaccataca cagatgacaa tcactaggaa actgattata gaaaaaagat
    20101 tcatcgtcta gctccttttc aatagattaa agaccggtga cctctcgaat gtagcttctg
    20161 gctttgactg catattcaaa tggattggct ttagccgggt cctgttctgc ttcaactacg
    20221 atccagcctt catagtcgct ggcatggatt gctgtccaga ttggagtgaa atcaatcatc
    20281 ccatcaccag gaaccgtgaa gacgcctttc ttcacgccgt ttaagaagct gtctttttcc
    20341 ttttctactt cttcagccgt ttcttgacga atatctttga aatggatgtg tttgatacga
    20401 tcttgatgac ggttgtaaac cgcgattgga ttttcaccag aaaagaccaa atgtcccgta
    20461 tcgaacaata atttaacttt gtctggatca gtcatttcca ttaaacgatc gatttcttcc
    20521 gtcgtttgaa cgccggtacc catgtgatga tggtaaacga tctccatatc cttttcgtgg
    20581 gccaaatccc ctaatttttc taatccttcc gccagttttt tccactcttc atcagtgaaa
    20641 actggtttat catcaaagac tggcgctgtc ataatccctt ggatactatg accctgctca
    20701 gctactacga tgactttagc gcccatcgca tgtaagaaat cccgatgtgc tttgaaggct
    20761 tcggcagttt cttcaaaagg tttagttgtc agaaacgcac tgaaccatgc actggcaacc
    20821 tttaagccgc gaatattcaa atattgttcc aacacctctg gatcttttgg atatttgttg
    20881 ccaatctctg ttccggtgaa cccagcaagg gccatttcac tgatacattg ttcaaaggta
    20941 ttttcactac caagctcggg catatcatca ttggtccacg cgattggtgc gatggctaat
    21001 tgaatttgtt tggtcatatt ctcacactcc tgcttatttt tttagttgta ctaaactctt
    21061 cgtatcgaat gattcttgac aagccaacgc gatcttagtt gattgcaatc catcttctgc
    21121 attaaccgct ggttgacgtt tttcttgaat acatgagaca aattcttggg cttcacgaat
    21181 gaaggcttct ctgaatcgtt ctgggaagtt ttgtgaacat ggacggatga tcccttgatc
    21241 gttcatgatc gtcacaagat ttttctcagg cgcttgcgct acccgcagca ttcctttggt
    21301 tcccatgatc tctgtttcga catgatagcc atgggctgcg tttctgccgg caactagtaa
    21361 agctattgtt ttatcgctca ggctcatcat tgcggctcca gtttcaagct caccagcctg
    21421 atccaattct ggatacgccg catttttccc aatcgcccac acttgatcga cttcttggcc
    21481 ggtaaaccag cgaaccaaat caatatcatg gatcgccata tctaagaaca agccgccgct
    21541 gtcgttattc atggcaaatt tgacaaagct ttccaagcct tcgctcggat cgatcccata
    21601 acaacgaatc agtgtgattt cgccgatttc gccatcttct accattttct tcgcatactg
    21661 atacgactca tcatagcggc gcatgaagcc taacatgaag acctgttcag gatgctcttc
    21721 gatgactttt tgggtctttt taatatcttc aacttcaagg cctaatggtt tttcagtaaa
    21781 gacatgcttg ccattttcca tcgccaagcg gacttggtca cagtgaaagc ctgatggtga
    21841 aacaataaag actgcatcga tcacggaact tttgaccatt tcttcatagc tggaaaaagt
    21901 atgcttgacg cctaatttct tctgtgcgaa tgctaattct gtttcaacta aactgcacgc
    21961 agccgtaagc tcacaacaac tgacacctgt tgctaaattt tgtgcatgta cttttcctaa
    22021 tcttcctaaa ccgacgatgc ctactctaat gttgttcatg aaatacctcc gttttctgag
    22081 aattactcat tgtatagtgc tggtcgttca tccatttgaa tcattacttt tgtttttgtt
    22141 tcacgagact catgacaagc catcatggtg atcgctgcta catacccatc ccaagcggta
    22201 ggaccgacaa ccgcatcatt tttgatcgaa tcgatccaca gcttgaattc ataatcatag
    22261 gcatccaaaa agcgggtctg ccaatctggt gaaacaggtg tgtaattttt ttcttccgat
    22321 ttaattcgtg tataggctgg gtccgtcatt cctacttctg ccgtttcgaa aaccacatca
    22381 catttaatat cgtagccaaa gcggcaattc acaaagattt ccaaatcaat cgttacaccg
    22441 gattttgttt gaaagatcag cagctgcgga tcatgcaaat cggcatggga gttgctggtt
    22501 tttttcggca agatcatttg aacagattca taatcttcat ttagcaacca gcgaagcaca
    22561 tcaatctcat gaatcgcggt gttgctgatc gccatcggtg ttgtgtagtt ttcatctgcg
    22621 ctaggatttc tatgggcaca atgcatcatt aatgcttctc cgtattgctt cgattcgatt
    22681 gcttctttca gttcaatgta gccgcggtca tagcggcgca tgaagccaac ctgaaccaat
    22741 ttcttcccat aagcaacttc cgcatcaacg atccggcggc agcctgctgc atccatcgct
    22801 aatggctttt cgcagaaaac atatttccca tgcttgattg cttctaacac atactgctca
    22861 tgggtcgggt cccaagaagt cacaacaacg acatccacat cttcagaagc gatcaatgct
    22921 tctccggttt caaagaattt agcatcgaca cgtttggcaa cctctttagc agcttttaca
    22981 ttgatatctg agactgctgt cacataagcc ccttgtgttt tctgattgat cctgtcgata
    23041 tgctcgatgc cgattgcacc tgttccgata acgcctactt ttactctcaa ttacttatcc
    23101 ccctgttctg ttgtttaagc acacatagta tgcgcttaca aaattattcc tgtgtagctt
    23161 ttattcttaa aaggtagagc cctctctttg ctccgccttt tactacccat aagcttgtac
    23221 gtataattcc ttaatttgac tatgactagg caccttttta ttattttgcg gacttccgct
    23281 ggtcaacgca tccgcggcca ttttgtctaa ggctgccata aacgcttgct gatcaatacc
    23341 ataatctcga atcgttggga tcgacagctg ctcacagagc tcttggattt ttttaatcag
    23401 ataatccgca gcctcttcca gcgataaatc ttcggctgcc ggataaataa atctggcgat
    23461 cgtcgctaac tcagtctgaa tagcatcctt tgagaattca aaaaagaccg gcaataacat
    23521 cgcatttgat atcccatgag gaatgtgaaa aagtgccccc accggtcgac tcattccatg
    23581 aatcaatgtg actgacgcat tcgaaaaggc aatccccgcc tccgttgaag ctaagctcat
    23641 ttgttctctg gcttcagtgt cgtcgctatt ttcatatgcc tgagaaagcg tgttcaaaat
    23701 tttcttcatc gcttccagtg aaaatagttg cgttacttca ttggctttga ctgaaacata
    23761 agattccaaa gcgtgacata gcgcatcgac tcccgttgcc gcggttactt ttggtggtac
    23821 agttttcgta aaatcagagt caacgattga gatctgtggc atgaaaacga gatccttcat
    23881 catcattttg actgctgttt ttgtattggt aataactgcc acatcggtta cttccgaacc
    23941 agttccagca gttgttgaca ctgcaatcag cggaatgccc gcttgcagct gatcaaaatt
    24001 tcttgcatga atcgcttcga gacttaattg attagcagcc aaaacggcta ccaccttggc
    24061 tgtatccaga cagctgccgc caccaaaagc aatgatatga tcacattgat gcttctgaaa
    24121 aagcttcagc gcatcataaa cattttcttg cgtcggttcg gcttgaatgt ctgtatactc
    24181 cgctaattga tcatctgttt ttcccaacag ctccttgatc ttttgaagat gcccggtctg
    24241 ttccataacc gtatcactaa tgattaacgg cttgttgcct agttgttctg attcttccat
    24301 aagaaattgg attgttccgc cgccaattct taaattggca ggcattttca gttgaatatt
    24361 cacagaaaaa acctctctct ggcttatagc ctataacaga atcctcgaag ctctttaaca
    24421 gagcttcgaa gcaaatcttt aaatctcact ttgcgcataa cgtgctgtaa caactttccg
    24481 atgggtataa aactcaacac tgtctttgcc attggcatgc aaatcaccaa agaaagagtg
    24541 cttccagcct gaaaaggcaa accaagccat cggtgcaggc accccaagat tgacccccag
    24601 catcccagca tcaacatttt ctctgaagta gcgaatcgct gatgcgttgt tggtatacag
    24661 acacgaacca ttcgcaaatt cagattcatt cgtcagctca acggctgcca tcaaatcttt
    24721 tacccgcacg attgatgcga ccggggcaaa aatctcatcc tgccagatct tcatatccat
    24781 cgtgacatta tcaaaaatcg ttggtccaac aaaatagcct ttttctggaa tgttttcgcg
    24841 accatctagt accagtttcg ctccttcatc gacccctttt tggatatagt caaaggtccg
    24901 ctgctgtgca tcttttcgaa tcactggtcc taaaaagacc ccttcgtcca agccattgcc
    24961 gatcttgatc gcttttgccg cagccttaaa tttttcaaca aactgatccg cgatttcctc
    25021 ttccaaaaca atgacggagg ctgccatgca gcgttcacca gcagaaccaa aagctccgcc
    25081 aataaggtcc ttgactgccc gatcaatatt ggcatctttc agcacaacaa catgattttt
    25141 cgctccagtc aacgcttgta cccgttttag attttttgtt ccttcacggt aaacgtattc
    25201 accgacattt tttgatccta caaagctgat gccttttacc agtggatctt gcagcaatgt
    25261 gttgacaaca tcgtgggcac catagacaac attaaaaaca ccgctaggaa agcctgcttt
    25321 ttcaaccagc tcaacaattt tctccatcaa caatggtgtt ttttctgaag gcttcagcac
    25381 caccgtattt cctgccgcca gagccatcgg gaagatccag aagggcacca tcattggaaa
    25441 attaaacggt gtgatccctc cgattactcc gattgggtaa cgataattcg tgatctcaat
    25501 atcagtcgcc gctgttgata aagaatctcc catcattaaa ttggtgattc ccgctgcgtg
    25561 ctccacattt tcgattcctc gttgaacttc tcccagtgac tcggacaatg atttcccatt
    25621 ttccagcgtg atcaaacgcg ccaattcact tttatgatct gttaaaagct gatgcagctt
    25681 gaacagataa cgtccgcgaa caggaacagg aacttttttc caagtttgaa acgcagcatc
    25741 ggcagcttta actgcttcgg ctgtctctgc tttgctagac aacggtacat tcgccagcgc
    25801 ttcttctgta gccggattca ttacttcttc aaactctgtt gagcgactag cgacccaacg
    25861 accatcaata taatttctta aaaatcttgt catttat
//
LOCUS       DANKMN010000043        25682 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000043 DANKMN010000000
VERSION     DANKMN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..427)
                     /locus_tag="PW224_001656"
     CDS             complement(<1..427)
                     /locus_tag="PW224_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2908345.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHN"
     gene            complement(440..850)
                     /locus_tag="PW224_001657"
     CDS             complement(440..850)
                     /locus_tag="PW224_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2908346.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1125..1337
                     /locus_tag="PW224_001658"
     CDS             1125..1337
                     /locus_tag="PW224_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2908347.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1340..1552
                     /locus_tag="PW224_001659"
     CDS             1340..1552
                     /locus_tag="PW224_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908348.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1692..1847
                     /locus_tag="PW224_001660"
     CDS             1692..1847
                     /locus_tag="PW224_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908349.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1844..2068)
                     /locus_tag="PW224_001661"
     CDS             complement(1844..2068)
                     /locus_tag="PW224_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2908350.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2146..2334
                     /locus_tag="PW224_001662"
     CDS             2146..2334
                     /locus_tag="PW224_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908351.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2331..2504
                     /locus_tag="PW224_001663"
     CDS             2331..2504
                     /locus_tag="PW224_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908352.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2660..2995
                     /locus_tag="PW224_001664"
     CDS             2660..2995
                     /locus_tag="PW224_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908353.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3228..4169
                     /locus_tag="PW224_001665"
     CDS             3228..4169
                     /locus_tag="PW224_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL2908354.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4171..5061
                     /locus_tag="PW224_001666"
     CDS             4171..5061
                     /locus_tag="PW224_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL2908355.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5104..5904
                     /locus_tag="PW224_001667"
     CDS             5104..5904
                     /locus_tag="PW224_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2908356.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5919..6770
                     /locus_tag="PW224_001668"
     CDS             5919..6770
                     /locus_tag="PW224_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2908357.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6971..7432
                     /locus_tag="PW224_001669"
     CDS             6971..7432
                     /locus_tag="PW224_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2908358.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7458..7910
                     /locus_tag="PW224_001670"
     CDS             7458..7910
                     /locus_tag="PW224_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908359.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8016..8210
                     /locus_tag="PW224_001671"
     CDS             8016..8210
                     /locus_tag="PW224_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908360.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8241..8534
                     /locus_tag="PW224_001672"
     CDS             8241..8534
                     /locus_tag="PW224_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908361.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8564..9004
                     /locus_tag="PW224_001673"
     CDS             8564..9004
                     /locus_tag="PW224_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2908362.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            9079..9546
                     /locus_tag="PW224_001674"
     CDS             9079..9546
                     /locus_tag="PW224_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HDL2908363.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10293..10523
                     /locus_tag="PW224_001675"
     CDS             10293..10523
                     /locus_tag="PW224_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908364.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10682..11515
                     /locus_tag="PW224_001676"
     CDS             10682..11515
                     /locus_tag="PW224_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HDL2908365.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11508..12923
                     /locus_tag="PW224_001677"
     CDS             11508..12923
                     /locus_tag="PW224_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2908366.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            12935..14464
                     /locus_tag="PW224_001678"
     CDS             12935..14464
                     /locus_tag="PW224_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2908367.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14382..15434
                     /locus_tag="PW224_001679"
     CDS             14382..15434
                     /locus_tag="PW224_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL2908368.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15436..15753
                     /locus_tag="PW224_001680"
     CDS             15436..15753
                     /locus_tag="PW224_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908369.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            15880..16503
                     /locus_tag="PW224_001681"
     CDS             15880..16503
                     /locus_tag="PW224_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDL2908370.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16518..17408
                     /locus_tag="PW224_001682"
     CDS             16518..17408
                     /locus_tag="PW224_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HDL2908371.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17431..17646
                     /locus_tag="PW224_001683"
     CDS             17431..17646
                     /locus_tag="PW224_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908372.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17658..17999
                     /locus_tag="PW224_001684"
     CDS             17658..17999
                     /locus_tag="PW224_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908373.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            17999..18370
                     /locus_tag="PW224_001685"
     CDS             17999..18370
                     /locus_tag="PW224_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908374.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18363..18758
                     /locus_tag="PW224_001686"
     CDS             18363..18758
                     /locus_tag="PW224_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2908375.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18760..19137
                     /locus_tag="PW224_001687"
     CDS             18760..19137
                     /locus_tag="PW224_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908376.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19138..19746
                     /locus_tag="PW224_001688"
     CDS             19138..19746
                     /locus_tag="PW224_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2908377.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19746..20087
                     /locus_tag="PW224_001689"
     CDS             19746..20087
                     /locus_tag="PW224_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908378.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20329..23118
                     /locus_tag="PW224_001690"
     CDS             20329..23118
                     /locus_tag="PW224_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL2908379.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            23108..23848
                     /locus_tag="PW224_001691"
     CDS             23108..23848
                     /locus_tag="PW224_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2908380.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            23845..>25682
                     /locus_tag="PW224_001692"
     CDS             23845..>25682
                     /locus_tag="PW224_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2908381.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9282 a         3685 c         5240 g         7475 t
ORIGIN      
        1 gattatgagg aagccctaaa tattcaataa tttggaactg actcatatca gataaatctt
       61 gttcatctat tgttctagtt aacatttcaa ttgcaaacat attagcttca gcttctatac
      121 cataaatcat acaactaagg ttgtttgctc taaaagctgc tgtgcaaata cctttatgga
      181 gtctaacgtg ggctatttca tgccataata caaatctttt catagaaggt gataggtttt
      241 gattaattaa tatcacataa catcttttat tgtgtatcgt ttgtcctaat gttttattcc
      301 ctaaatcagt ttcaattaaa tctatatcag aatatttgat tattttatat ggattaatcg
      361 catcagatcc aaactctaca aataattttt ctatttcttc taatatccag tatttgttta
      421 aatgcatacc aaagcccacc tattgacgat attttttagg agtgaatttt ttctttgctt
      481 taactttggt taaggcaagt gcaagttgaa tggcatcgta taatttttcc ttatcttcat
      541 cgtctaaagg ttcaccataa taatttgttt cagcatctga attcaagcct tcaagtaatt
      601 tttcagcttg taaagcaata tcttgtttat ctttttctgt taaatcataa taatggcgtt
      661 tatcagtacg tcctaaaaga taatcagttg atacattaaa gtaatctgca actttttgta
      721 ttttatctcc agatggagta cttttattcc atctagacat gcttccattt ccgaaattta
      781 actttctttc aagttctgcc aacgtcattg aacgttcttt taataagatt tttatacggt
      841 ctaatagcat aggatatgca ttcctttcaa aataccgaca aaaaaagtat ttttctgcaa
      901 attatctatt tacaaataga gaatttgcag ttatactagt tttgtaagca agatatttac
      961 ggcatacaaa gactaaaagc aataaactaa tagtctcccc agacatcagt aagctcgctt
     1021 tttgtgcgct gtattttgta tgcttacata atagcatatt tgcagttttt gtcaaactta
     1081 ttagacaaaa atattagaaa atattcgata agggggtatg aagcatgact ttaaaacagc
     1141 aagtattaat tgctttagta aagaaaggtt ggagtcaaag agagttagct cgacgaatgg
     1201 gtatatcaat tacttattta cgagatatct tcatcgaaaa aagaaaacct aaggaacgtt
     1261 taaaacaaat cgaagaactt ttggatatca agttagaagt agattctaat gatcagccat
     1321 cagaacagga ggcgtaaaaa tggtacaacg tttgagcaaa gcggagttta aagagaaata
     1381 taagtattct gaaagtactt atcaaagaag aatgaaggaa tttaaaaaat cacgttttag
     1441 tgaggggtat aacgcagtta cttccaaaga aataacaatt gatgtcgatt tatatgaaca
     1501 gtttagaaca tggcgttcta aaaatcgatt tagagttaga gctgtttgtt aatagctaaa
     1561 aaatctaaat tcaccacttc tgaaaagagg agttgctgct ctcctttcaa gtttgccaaa
     1621 gtccacgttt tttcattttg gcaactcctc ttcttggagg tggtgacggc gagagacgaa
     1681 ggagggatga catgaaggaa gaatcaaaaa tagggatttt gaatgagaac aattacacaa
     1741 gattaattat tgaaacagaa gaagggaaaa aagtagcaga agttacattg accgatgcaa
     1801 ctcctgctga cggttatcgt attcgattaa ctcctaaata tgattaacct tttggtgggt
     1861 gtggatcgtt tccgtgacta tcacggcttt gaatttttcc atctctgcca tgaataaata
     1921 gctcagatcc ttgattttta gaaatctctc tagcgatttt tactgcttct gattttgtat
     1981 tagtatgtgc agtcgcttta gaatttccag cacctttgac attccaacca ccattggaat
     2041 taggtacaac atgttgattt tttcccatag ttttcacctc catttctata gaaatatttt
     2101 atcaaataga aatggagaaa ttcaataaac gaaaggagaa gtgatatggc atataccttg
     2161 caacaagaac atcaaattct cggtttgatt aaacaacgca ggaaacaatt acaagatgac
     2221 cgtgcagcgc ttagaaaagc cgatgagcta tcagataggc aagctgaact aattgcttct
     2281 gaacttgagg atttgagaat gctagaaata aaaaataggg agattagatt atgaagaaga
     2341 cagacacact ttttatagga ttcattttgg ggttattagt gattgtagcg caccaaagta
     2401 ttatcggggg aagcttgttc gcagcattga tggttttaat caatctgctt gattcaaaag
     2461 aaaggagcaa ctatggcacg agaagaagcg ctaaaaatcg gtaaagtgat tgctgataat
     2521 cggtggggaa atagccgccc tattatttta agcaagcaac atatcgaaaa gcaaaaagca
     2581 tggcaacaaa taaaaaagtg actctgccag ccagcaaaga gtcacataca aatttataaa
     2641 actaggagaa ttatatcaca tgaataagaa aattgaaaat cttatcgaag aattgaaacg
     2701 tgaatgccaa aagcaaggtg tttctattat ttgtacagcc caaaaagaag gggaacttaa
     2761 aagtctcgtt tatggtgaga caacagaaat tttactttgt cttgctatgc aagaagaaca
     2821 tttagatgaa aatctcccat tgtcagcaca tattatgcgt agaattgctg tggatgctta
     2881 cgaacaagcg aaaaatgaag aagaaaatca accttcaaat catacttttg ttattaataa
     2941 caaggaagat ttagctgatg tgatgactcg tattctcaag ggggaatttc aatgatgaat
     3001 gaaaaaacaa ttgatctact cattatttta tttttcacgt tagtgattgt agcgaaaagt
     3061 tatgtaatct ttggcttgtt attcgcaatt accatgcttg tgctttgcgt gttagcaagc
     3121 agaaaagagg ttaaacatga acgagttagc aaatcttgac aactatttaa ctgatcctga
     3181 ttatgctaaa ccaccatatg aagcaccaat tgatgaggag gatgaagatg agtaaatcta
     3241 ccttagaaat gagccatcaa gaatggcttg aagaccgtaa gaaaggcatc ggaggttcgg
     3301 atgttggaac aattttagga ttgaataaat ggaaatctcc ttatcaacta tggctagaaa
     3361 aaacaggaca agtagtactt gaagaatcag gaagtgagcc cgcttattgg ggtaatgttt
     3421 tagaagaagt ggttgctaaa gagtttcagg aacgtacagg caaaaaagta cgcagaagaa
     3481 accaagtatt tgaacatcca ttacatccat ttttaagagc aaatattgat cgggaagtag
     3541 tgggagaaaa tgccattctg gaatgcaaaa cagccaatca atttctcggt aaagaatggg
     3601 aaggcgaaga ggtaccgctt agctatctct gtcaagttca acattacatg aacgttctaa
     3661 acaaagacta ttgttacatc gctgtcttaa tcggtggtca aaaatttatc tggaagcgga
     3721 ttgaacgaga tcaagagctg atcgatacaa tcactgaaca attagtagag ttttgggaaa
     3781 caaacgttct tggaggtatc gagccaatta ttgatggtag tcaagcgaca gctgatttct
     3841 tgaaagagaa gtatgcagat gttgaagacg ttcaaacgac gttaccaatt catttcgatg
     3901 aactagttga acagaaaaac gaactcaaac ggactaagaa agaaattgaa tcagctatcc
     3961 gtcaagtaga caacgagatt atcagcgagc taggtaaacg tgaagccagt atcggtatca
     4021 ctcaaaaaaa catcatcagt tggaaatttg tccgtacgag acgtatgaac tcgaagaaac
     4081 tagcagagaa atatccagat gtcgcaaatg atgaagagat ttataacgtt actgaatcaa
     4141 gaaggctaac cgaaagggag atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa
     4201 ttggcagcaa agccacagaa acaagttgca ccaggtcaat taggacttaa agctttgatg
     4261 aatacaccaa caatgagaaa gaagttcgaa gaggtgcttc atgacaatgc taatgctttt
     4321 atgtcgaatg ttatgactct tgtatctaat gacagttatc ttgcagatag tgaaccgatg
     4381 tctatcatga gtggtgcgtt aactgctgca acattaaatc ttgggctaga taagaattta
     4441 ggttatgcat atttagttcc attcaatagt aaaaacaagc aaacaggaaa atgggaaaag
     4501 aaagctcaat tcatgcttgg ctataaagga tatatccaat tagcccaacg atcaggtaaa
     4561 tacaaagcat taaatgtgat cgaagtttac gaaggagaac taaaaagctg gaaccgactg
     4621 acagaagagt ttgagtttga tccaaatggt agaacgtctg atgaagtcat tggatatgtt
     4681 ggctatttcg agttactgaa tggattcaaa aaaactgtct attggaccaa acaagaaatc
     4741 gaagctcatc ggattgctaa caataaagat cgcgataaga caaagttaag tggtgtgtgg
     4801 gcatctgatt acaatgcaat ggcacgaaaa actgttttga gaaatcttct ttctaaatgg
     4861 ggaatcttgt ccatcgaaat gcaagaagcc accacatcgg atgagagagt ccaaagggtt
     4921 caagaagacg gcagcattat tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa
     4981 gaagcagagg ttatatctga agaaaatgaa gatgtacaaa ctggattatt tgatgcatct
     5041 aatccgccgt taaacaaata atgagggagc tttctccctt gcttttctag aggagaaata
     5101 cgaatgaaca caggatatgt caaattacat cgaaaagtga tgaattcatt cgtttggacc
     5161 aatccttata tgtataaatt atggaacttg tgtttgatga aagcgagtca tgagaatcgc
     5221 aagatacttt ttaatggaaa agagatacaa gtgaacagcg gagaattcgt cacagggcgc
     5281 gatgctatca catctgagat gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt
     5341 tctgtatgga gatggctaaa acaatttgaa aaaaacggaa tgttgaacat caaatcaact
     5401 acgaaataca gcgttatatc aataaaaaac tggtctttgt accaaagcag tgaacaacaa
     5461 atgaacatca aacgcacaac aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat
     5521 tataagaatg aaaagaataa taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac
     5581 gacaccgact cagtttacta cattctcgcg gaggagttat tcaaacagat ttgtcagaat
     5641 caggaaatca aaaagccgaa tctgcaaagt tgggcagatg acattcggaa aatgatcgag
     5701 atcgacaagc ggaccgaaaa ccaggtacgg ggaatgattg aatggagtca gcacaatgtg
     5761 ttttgggcat cgaatatttt atctgctaaa aaattgcgag aaaaatacga cacaatggca
     5821 gcgcaagcaa atcgtgatta taaaacaaaa caaactaaaa cgcttgaata cgagaaattt
     5881 agcacagatg agttgcctat ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca
     5941 tggaaaagct gatacagaaa gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc
     6001 ctttgtatgg atggcgtacg aagaaccctg atggctcaga acgttgtaag ccaacctgta
     6061 tgcaatgtgg gtataaagca ttgcgagttc aggaagactt acaaacagaa cgaatttaca
     6121 acgagagcct gaaagcaaga gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc
     6181 aagcgttgtt tgattgcaca ttgcagaatt atcaaattgt cgatcaagaa acaagacaag
     6241 cggttgaagt aaccaaacgc tttgttaatt cagtcttgtt aggaaatcca agtcaccttg
     6301 ttttaactgg aaaacaagga acaggtaaaa gtcacctagc gatggcagcg gcttgggaag
     6361 tcttgaagcg atcaaactac gataaaaaaa tcttatttat cgggttacag gaaatgctgg
     6421 atcaaatcaa attttcgtac aacaatcctg aactcagaaa aacgattgag ggatcgttga
     6481 ttgcagatat caaaacagcg gatttagtca tcattgatga tatcggttca gaactaggaa
     6541 aagatgcatc agatagtcga gcgtttggca taaacacgct aaattcgttc ttggatgcac
     6601 gacagaacct agcaacgatt atcacaacga acttgcttgg tgaagaactg aaaaaagctt
     6661 acggtacgag aacaatatca agaatgtttg tcaactctga tggatttacg atggtatttt
     6721 ctcaaacagc agacaagcgc ataaaaccag tcaaaggagc gatcgcatga atttgactaa
     6781 acaagaaaaa agaatctgtc gtcagataat gttgcaaatt ataggtgatc gtgacatgga
     6841 cgaattatct caacaagaga gtcgaatggt tcggaagata agtaagctaa ttatttatcg
     6901 ggatacagaa agatttctca ctgttttagg taacgtctac gagaatccag agttattgga
     6961 ggtagagtaa ttgccaaaaa tattagatgc atgttgcggt agtagattgt tctggtttga
     7021 taagaacaat tctgacgtaa catttatgga ttgtcgtcag cagtatgaag aattatcaac
     7081 aggtcatgtg atcaatgttg atccagatgt ggtcgcagac tttcgcgata tgccttttga
     7141 agatgatgtg ttcgatatgg ttgtatttga cccgccacac ttaagacatg tcggcgaaaa
     7201 ctcatggctt gccaaaaaat atggaaaatt agacgaactt tggccagaag acatcaggca
     7261 aggttttgca gagtgtatgc gagttttacg accttcagga acattaattt ttaagtggaa
     7321 cgaggaacag ataccacttt ctgatgtgtt agaggctatt ggagaacagc cattgtttgg
     7381 taataaacga agcaaaacac attggcttgt gtttatgaaa aaattcagct aacggaggaa
     7441 acaaaggagg aaaagtaatg aagattgtat taaataaatg ttttggtggc tttgggttga
     7501 gccatgaagc aaaaatggaa atttttaaaa gaaaaaatat tgaagtattc ccatacatca
     7561 ataactttag ctatgactca gatgatgaat acacgagata cacaggccaa aagctaggtt
     7621 cgatggattt tatctactat ttcaaaaaag acccgaaaat cgacaaagtt acaggaattt
     7681 atagcgaaat tgaccgactg tacggaattg ctgatgattc cagtttttca agtgattcaa
     7741 acagaggtga taaggacctc gttgctgttg tcgaaaaact aggaagtgag gccagtggac
     7801 catatgcaag tcttaaagtc gttgatatac ccgatggggc tgagtgggaa ataagtgatt
     7861 atgacggcgt tgaaactgca cattatggtt ttcaaacagg tagtatttag tccactaatg
     7921 acaactaaag aggaggcaga aggatgagtg aagatgaata ctttgaacag tagtctatta
     7981 acgacagaga tttttaactg gaaaggggtg tagtcatgga cgcatcggaa gtagtaaata
     8041 aaattaatag tctcaaagag gttttcggag acgtcgaagt agtgataaac gcacaaggct
     8101 atgacaacga attttttaag cagattaccg acattagcat tcagcaagga atggaagacg
     8161 aagacggaaa ctttatcgat gaagtagcca ttttagtgac ttgcgagtaa gtaattgaca
     8221 gctaaagagg aggaagcgga atgaaactaa aagacggatt ttactccagc ggccacggca
     8281 tcggcggttt aatgctagat atgccgacaa agaatcctaa aacacgtaag aaaccaaaat
     8341 tcaaagtcgg tgacatggtt cgctgcgaag cagaaggatt catctatcca tttcgtggat
     8401 atgtagaaca cgtctataat cactcagcaa tcattcgcat tgaaaacacg atggaatgtg
     8461 ataagtggtt agcgaaaagc aaagagaatt tagcagtggt gagattggtg gacatggaag
     8521 ttataaaatc ttaattttgc ttttttacaa ctatcctaca acaatgagcg ttattccaca
     8581 actggaagga gaagaggcaa ttgtgaacat tttagagaat atagatatta agcaaacaag
     8641 gaaaaatgct agacgattac taaaaagata cagaaattta gaacgtttgg tcggaccagt
     8701 gaaaatagac ttttccgtga tgactgttac caaaaatttg aaattcacaa ttgacagtca
     8761 aaacgaagaa atcattgaag cgataagtac tcgagattcg gttatcgaag cattaacacg
     8821 gctaagtaga atccatttcc aggtacttta ttatagttat tgttttccta ataagatgtc
     8881 aatgtatcaa ataggagaaa aattaggata ttccgataga actatcgaga gaatgaaggc
     8941 agtagctttg gttgaatttg ctgaggcgta taaatcagga gaactcattt cacgtacaaa
     9001 ataaaaaaag tcggattcct ccgactatga ataatatttc cgacataagt attatatcat
     9061 aattggggga atcagaggat ggtacttttc gatgtaaaga aatatgagac accaagcgcg
     9121 aaagatgttg atatggaacg cacaaaacat aatgtcgctg tgtttctttc agcatatcta
     9181 tcagctagat gtagagtagg gcaacatcga gaaccaaaag tgacagcatc ttattccttg
     9241 gttccacctt ctacagctga tcatatattt gaagcagaaa gaatgctgat cgataaagaa
     9301 gaagcacagg aagaatttga gtatctgcat aaattgtttg ttcgaggcta ttcagcgata
     9361 cagcatccgc ataagcctga tgtgactgaa aggcgcaaga agatattcta tgatcgttat
     9421 atcaatggtc tgcccattta tgtaactgct caaaggaata atactagcga agaatcggtt
     9481 aaagtagaat caaacagaat tatcatccaa tttgcttcat cgttagaact ggttgctttc
     9541 aagtagccag tttttacact ttttataccc ttttattacc aatttggttt ccattttata
     9601 ccttttttat accaatcact tacctattca acgttgtatt atgatagtgt cgaaaaatta
     9661 ggaaacagga tcgacaaaat aaaatgtaag ggaggaaatc tccctcatcg tttaattaag
     9721 cttcgatagg cagcaacgga aatattaaga ataaggatgt gaatttcaac tccttctaaa
     9781 ttgttcttat tatctatcat ccgttgctgt ctattgtttt tttaattatt cacacgataa
     9841 ctaaaggtaa gtgaagagaa atgattccat taataatttc aatttttgcg ctttgtctta
     9901 atgtatatat gattggattt aaaaatgggc aaaataaaaa atagtagcag ccaagaataa
     9961 ttttatagtg tcactgtggc ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct
    10021 tcggcaacca tgcaaggttc gattccttgc cagtgacttt ggcaaccgag gcatcggcgg
    10081 tttaaaaata taggggtgcg caatttcgta cgcgttttgt gcatcgtgta ggttgctatt
    10141 acataactgg ttaggttaga ttgagatttg ggattcggta caaatgaatc gtcaaatgtc
    10201 tcaagcacag gatcggaaac gtccctgcct gtgcattaca tattagatca ctctttgagt
    10261 ggtctttttc tttacataaa ggaggctaca taatgagaaa ctactggtat gtatcgctaa
    10321 ctaatgaata tcctcgaacc attgatgatt gttcagtgcg tgttgtgcgt tctgtacaaa
    10381 tcaaagggaa gtactctatt gtcgaaatgg ggagagaagc agaaccatgt gagattgatg
    10441 cgtgcaagct cgtttattgc ggtcatggtt tctatgatga accaaacatt caaaataata
    10501 ttaacaagaa tttgagggat tagaatgcaa gaaatggctt aaaaatctcc aatcaggaca
    10561 aatcaagtta gacaaagttt ccgatttaaa gatattgatt gaagctgatt taatgctaaa
    10621 agatattgaa aattagaaaa caaaactcaa cctaaacaga atgtgaggtg gtgtgtattg
    10681 aatggcaaga caacgtgatc caagacgtga tgaagccaaa agaatttggt tagaatccaa
    10741 cggagaaaag cagttaaagg aaattgcatc tgaattaaat gtttcagatt ctcaggttag
    10801 aaaatggaaa tcgcaagaca aatggagcgc tgaattgatt agtaacgtta ccaatggcaa
    10861 aagtaacgtt actaatcaag gtggcgctcc tattggtaat caaaatgcta aaggtaacaa
    10921 aggaaatagc cgagcttctc cgccagtggg taataaaaac gctttgaaaa caggtgagta
    10981 tgaaacaata ttttttgaca cgttaaccga taatgagaag gacatctatt ctagtttgaa
    11041 tgatgatcct tcttttgctt tgtccgaaga aatacgactt cttaaaataa gacaacttcg
    11101 aatgatgaaa agaatccaac aagctgaagc tggactaaat gatgaagaag tcgaacgatt
    11161 gcagcagcta agaaagatta aaactccgat tgaaaaaaat ggtaaaaagc tagaaatcaa
    11221 gcgtgaggtt atgcaagatg tgcagattag cagaaaaaaa catcgcaaaa ttgatgatat
    11281 tctttcaatt gaagattcat tgactcggat tagcaaccag ttagctaaag ccatcaagca
    11341 aatgaatgaa ctttatatga atgaatacag aactgattta attaaagctc agactgataa
    11401 gatccaagct gagacaaatg aaattggcgg aaataattca ggtgaagaaa tagaagaatg
    11461 gaaacaggca gttttaaatg ccgcaaacaa acgggcggtg aaagaaaatg aataatgaat
    11521 ttattccttt tgctgatatt ggttctgcca ttgattatta ctatgataaa ccagtagctt
    11581 tttgccaaga tattttgcat ttgaatcctg atgaatggca agaaaatgtt ttaaatgatt
    11641 tagctgaatt ttcaaaggtt tctgttcgtt ctggtcaagg agttggaaaa acagcattag
    11701 aagcaggagc aatactttgg ttcttaacgt gtcgacccta cgctaaagta atagcaacag
    11761 ctccgacaat gaagcaactt tacgatgtac tttgggcaga ggtagctaaa tggttaaatg
    11821 atagcttgat caaaaactta ctgaagtgga caaagaccaa aatttatatg gttggtgatt
    11881 cagagcgttg gtttgctacg gctagaacag cgactaaacc agaaagtatg caaggttttc
    11941 acgaggacca tatgttgatt gtggtagatg aagcttctgg tgtgtctgat ccaattatgg
    12001 aagctattct tggtacgcta tcaggttttg ataataagct gttgatgtgt ggaaacccca
    12061 ataatattga aggtgtcttt tatgattccc ataattcaga ccgtgataaa tacagagttc
    12121 ataaagtatc aagctatgat agtaaacgta caaacaaaga caatatagaa atgattctta
    12181 aaaaatatgg aaaagaaagt gatgttgctc gtgtccgtat ttttggagaa tttccgaaag
    12241 gtgcgttgga ttcatttatc agtcttgaaa cagttgaatt ggctacagaa aaacaaatta
    12301 gtgattcttt agtcaataaa acaaaggttg ctcatattgg tgtcgacgta gctcgatatg
    12361 gtgatgattc tacgattctc tttcctagaa ttgctaccag ggcattggag tatgagaagt
    12421 attcaaaacg tagcaccatg gaaacaacag gatatgtcat caacatggcc aagaatctaa
    12481 tgagtcaata tccgagtatt gataaagtga tgattaaagt cgatgacact ggtgtcggag
    12541 gtggtgtaac cgaccgccta gaagaactta tagaagacaa acattatcct tttgaggtgt
    12601 ttggagtgaa taacggttca acatcagaag acgattttta cgataattta ggtactcaac
    12661 tatggggaaa catcaaggaa atgttagaag aaaatatgac agcaaatctt aacggagaac
    12721 agcctgttat tgaattgcct tctgatagtt cgttaatcaa agaattaagt actcgcaaat
    12781 tcaaaatgac aagtagaagt cgtatacgtt tagaaagtaa agatgatatg aaaaagcgaa
    12841 atattggtag tcccgatatt gctgacgcac tggctttagc gttttatgag ccaccaagtc
    12901 actatcaatt tattcaattt taggaggtga gcttttgatt agtacagtat tatctttaga
    12961 gagatataag aagctacgtg ttaaatatgc aacacaaatt gaagatggaa tgtttgatcc
    13021 gaatggtttt atagaagata tgaaaccatt ttttgctgat cgcgaaagaa aatatctagc
    13081 ttatactagc gaaaagaatg aaatagataa tagaccaaag cctaacactg atattgtaaa
    13141 agttaataat aaacttcatg ctggtatgta ttcaattgtc gtagaccaag cagtcaatca
    13201 ttttactggt atacctatca aatgggatta cgatgtatcg gaacaaaaaa gaacactcat
    13261 tcaaagatta aaagataaat tcttaaaaaa tgatatagaa cttccgacag ttccagaagc
    13321 ttttaacaaa ttaacaagca atcttgattc catgagattc gcaatgcttg attctgaaac
    13381 tgcaactttt caaggagctt gtggagtagc tttccgattg ctagagcctg tggaagaaga
    13441 tgatggttgg aagttaagag caagtaatat tgaaccttgg agagcagaaa gatatggaaa
    13501 tgccggaatc tatatcaaag aaaagtatga ctcttaccag aaaaaatttt ttcaagaaat
    13561 gaaagttatg acaagaaata aaattctgac atatgcttgc tatggtgatt tgaattttgt
    13621 tacaagcgga acgttcaaaa aaattgatga gaccgataac cctttgggga cgattacctt
    13681 ggcagaattt aaaaataata cgaatcgtta ttgtgatttt gaagtagctg aggaaattgg
    13741 agatgcaatt gatcgtgctt tgtctgatca acaaaacgaa atcgaacaat ttaagcttgc
    13801 gtacatgctt gttactggca caacaatgag caaaggcact gctaaagaaa tgatgaatca
    13861 gctagggatt atcaacttaa aagcccctac tgcaaaagct gaatatgtca caaaaaattt
    13921 agcaaaagat ttcaatgaat atcatatgga tctattgaaa aaacagtttt acaccatttg
    13981 taaagcaatc gattttaacg atgaggtgtt taaatcaaac agttctggag aagctcgcaa
    14041 gtggcaaatc attagcctag aagcgaaaac aaatactaaa gagcaatatt ttagagaagg
    14101 attgaaagaa tgcgcagaaa caattgctgc ttttcttaag tttcatgaca aagtagaaat
    14161 tgagcctgaa aaaattattt ttactttctc tagatctttg ccaactgatt taagttattt
    14221 agctgaagct ttacctaaac ttgcaccata cgtatccaaa cgaactattc agagccaaat
    14281 tccatttgta acagatgttg attatgaaaa tgaaatgatg gaattggaaa gtggaagtgc
    14341 ttatccagat agtgaataca attttggcgg aggtggcaat agtgacgata gaaacgaagt
    14401 attggaccaa acgtcgagaa ctggaggatc aagcaaggct caaacaagaa aatcagacac
    14461 ttaaaaaatt aactagtgta tttcctgaag cactgaaaga gatacaagca aaactattat
    14521 cacaagctga cttacacaat atcacttatc cagaaatgat ggagttttac agtacaagta
    14581 atcagaaaaa atatcgtgaa tatgtggaaa aaaattataa gtcattaaaa atgtatgatg
    14641 caaaatacaa agagtttatc gatgaatttt ttccaccatt tgactatgca aaagtcaatc
    14701 gcttattaca aatacgatca gatgtattta aaattcttgc agaatatgcg atggatgcag
    14761 atgtgaatca atatttttct gatcgcttag aggaaattct tcaaagaaca tattcttcta
    14821 atgctaatgt ttttgttcaa cttttaaacg ttgatatacc aaattattta ccagaaaatg
    14881 aaattaaaca ttatttgaac tatccatggt caggaaagac attttcaaga agactttggg
    14941 gaaatatttc atcgcttgaa cagaaactct ctaatgctat tgtaaaaagt gttgctagtg
    15001 gagaaggcgt tatacacgca ttaaacacca tgagattaga ctcagaaatt tgtgacatgt
    15061 ttaagttaga agaatcaaag tataacaaag cgatagaaaa tctcgttcga acggagtatg
    15121 caaaatttgc acaagatggt attgaaaaat catatttaga aacaggtatt gaggaataca
    15181 acgtattgac tgcaaaagat gagagagttt gccggatttg tggaggaaag gcaagtaaga
    15241 atccctataa actgaaagat gctgtcatag gtgaaaatcg agcacctttc catagtcgtt
    15301 gtagatgtac ggatgttcct aatttaccaa aattaggaaa ggatattgat gaagaatatg
    15361 accgtttatt tggcgattta ttagatgagt ttgcacatga ttcttttgga attaatttga
    15421 aacggaggaa gtagaatgaa agattttttt gaagcagtac taacaattaa tgtaaatgct
    15481 gatattgcag aagcctacaa aacagctatt gagtctgaga accatcctaa tggcttgaga
    15541 gaccattgga atggcaatta tgcctacgtg gttattggcg atcaaactgt taattatcaa
    15601 gataatactc cagttgataa gaataccgtt aatttaacga ttcaattatt atctcattca
    15661 ttaccaaatt taaaagaaac agttgattgg tatgaaaaga tgggatgtat tgttgttaga
    15721 actgactaca aagaaggaaa gtctagtaat taggcttttt tattttgtcc gaaatgacgt
    15781 taaactagcg caatactggg cttggttgaa tggtggggcg caactattaa aactcaaagc
    15841 aatgcggggc gtgaaaacga atcgtggggc gaaaggagaa tgagcatgaa acacaaatca
    15901 ttaatgccaa tgaatttgca atactttgcc gaaggtgacg atcaaaagtt ttcttttgat
    15961 gacttcaaat cttttgtgga atccaatgaa gaagctcaaa aatttattca gtcacaatct
    16021 caaagtgttg ccgacaaaca attggaagct tggaaacaaa ataacttaga taaaattaaa
    16081 caggatacca tcaaggaata tgaggaatct aagaaaaaca agtcacctga gcaaattcaa
    16141 ctggaaaaac tacaagctga atttgaagca gaaaaagcgt tgcgtgtgac aagtgataat
    16201 aaagcatttg ttgcagaaca aattgctgga ttagaactag atggagagtt aaaagagtct
    16261 atttctcaat ttatgctaaa taatcttgtt agttcggata cagatttcac taagaatgct
    16321 gttgaaggtt ttacaagtgt tttgaatgca attaatgaga aacatgcaga tgcattaaaa
    16381 gaactacaaa tgaagtctgc atttggtgga actcaacaat cgaataacca ggttcagcag
    16441 aacaatgaaa cattaacaaa tccagaagaa caattaggac aaattcttca acaatttaac
    16501 taggagagtg aaaaattatg aaaaaaacat ctttaaataa tttagagtat ttggatattt
    16561 caccagcgat taatgctatg caagtaccaa atacaccttt tttaagctat ttacttggtg
    16621 ctggaaaaac agagccagca aactcgacag aaattaaatg gcgtgaatat gatatcaaca
    16681 acgatgattc ttctgaaaaa cttgagggcg gagaatatcc agatgctgaa tcaggtcgaa
    16741 cttggtttaa caactatact gaaattttta gaaaatcaac ctctgtatct ggtacattag
    16801 atgctattaa tgtgaatgga gtcggaaatg aattaactaa tcaagtagca ctacgtggta
    16861 tggaaatgaa aattgatttg aaccgaaaat tgattactgg tgtaaaagct gatgaaaatg
    16921 gttctaaagg tcgtcgaatg aatgggattt tgaacttgat caattcagca aataaggcag
    16981 aaacagccac tgcgggtgca gtaacaagaa aagatatcga tactttattt aaattgatgt
    17041 atgaaaaagg ttatatggga gaaaaactat gcttgatttc tccagatatg caggagttaa
    17101 tgactgatga gttagatgga aaatcaacaa aaattgttca gttcggggaa agagtaactt
    17161 ttggattgca aattggaaat atcgtgtcta attacggtac aggtattgct ctactagaac
    17221 catcattgcc aaaaggaaca attgccgcaa tcgatactaa ttatgtgaaa ctacgtccat
    17281 tacgtgaatg gagagcagaa gaacttgcaa aaacaacgga ttctagacgt atcggtcttg
    17341 taggtgaata ctctcttgaa tacaatgctt caaactctgg ggcaatttta aatttaaagt
    17401 ctgaataaaa gggagttagt actccctttt ttgataggag gaattacagt ggtaaaaaaa
    17461 gatgaaacta aaaaagatga agtcgtgaaa tatagagtag gtaaaactaa aaattttgtt
    17521 gggtttgttc atcctaaaac tcgtagattt atcacagcag attcaaataa cgaatttatc
    17581 atttctatag atgataaaga agcaattgca attttggaag atgcaattga tgttaatgaa
    17641 atttaggaag tgatctgatg gatgaatcgc taaaaacgga aatcattgag tctacaaaag
    17701 aagattttcc agatttgagt gaagaacgca taactaattt attagaaata attttgctag
    17761 aaattgaatc atacaacact tgtaaaaatg atatttcatg ggaaaagtta aaaagcgtga
    17821 ttagtgaagt gttgtataaa ataataaaaa atgaatcaga aaaaacagta tcttcagtta
    17881 gacgtggcga tacaacgatt agttatgctt caacaacaaa tgatgttagt gagttacttc
    17941 taggctatgg tgatttgata cgaagagtta ttggttgtgg aggattggag tttttttaat
    18001 gaatgaagca gatattttag aaactactta tgaagatagt tgtattattg aaagactgac
    18061 ggacattgaa gattctaata caaatattac tattcaagat tacaaaaaag tatatgataa
    18121 tcctatttct tgcgctcttt cacaaggtca aatcgatgga ctagcagtca tagaagatgg
    18181 agagatggta aatgtttcaa ctgacacata taaattattt gttcatccta agattaaact
    18241 aaaaaaagga gatcgaataa caataactca aaaggccagt ggcttaattt tttctctatt
    18301 tgctactaag cctttttact atcctagtca ttgtgaagtg aatttgatag gaagtgagaa
    18361 aaatgggtaa tctcaaatat gaatcaaatg cagaaaagat cattgagaat tttaaaaata
    18421 tgactgtaat tgctcaaaaa gaaggaatat ccttcgttaa cgattcaatg aataaagtcg
    18481 ttagtttagc taaaccatta actcctgtaa aatcaggtaa tttaaggcga ggatatcgtg
    18541 tagtaaaagc tagaaaacta tcaagcggtc gaattgttgg agaagttata aacaatgaac
    18601 attattttaa atatgtagaa gaaggtcata ggactaaaaa tggtggcttt gtcaaaggga
    18661 aattcatgtt aactcgtgca acgaatcttg caaatatgtc ttatattcct cgaagattta
    18721 aacaaatggc aataaaaatc gttaagaaag gaaagtaaca tgaaagatga aatcattgct
    18781 gcaatcagta gcaaattaaa agaaatctat cctgatggga caatatatct tgattcagtt
    18841 atgcagtcaa ctaaagattt ttatttcgtt ttatcagtaa tggaatctgg aactgaaaat
    18901 gtaggaattg atattcaaaa tgtttctttc ttaattgata ttgcattgat tgataataac
    18961 cctaatagaa atttaattaa tgaattagtc tcacgttgtg ggactttttt taatacgatt
    19021 acaatcgatg agcaaatatt atttccaaaa gcctatttac ctgatgaagc agatggtgtt
    19081 caacatattc gttttacatt agaatttcca caatatattg aatggagtga aagataaatg
    19141 ggagaaaaaa gaagtaaaac cggaattatt tctgtcgaaa aacctacttg gtttcctttg
    19201 gaagatgaga cagggaattt tccggtatat ggtactgcgt tgccaatggg aacagcagta
    19261 agtatcaaac caacagctaa ttatgaaaca acacaagatt atggggattc agtggttcaa
    19321 gatcaattta cagcttttgg tggtgcagaa gttgaactgg aagcaaatgg atatactcat
    19381 aaagttttat ctacaattac tggagggaaa attgttaaag gtggtgcctt gcgttctgga
    19441 gaagatattg cgcaagatgg tgcgtttgca tatagacgta aaaaatcgaa tgggaaatac
    19501 cgttatacgg tcttttataa aggacagttt gctctagatt cagatgaaac atcaacgatt
    19561 gaaggaagta aagtaagttt tactcatcca acttggaagg gttcatttgt agatgtacca
    19621 ggacttggat atatgtattc agttgatgaa gatgatgaag gcgtggataa agcaatgatc
    19681 ggaaattggt ttacaaaagt agcaattcca attgaagaag cagaactttc aggaggtaca
    19741 gaataatgtc aaaatatcaa acaacgatta aattaacgaa aaaagacgaa gaaggtaaat
    19801 atgagcaagt acaatttaaa tctgctgaat ttttaccagg aacagttgta gaagatgctg
    19861 caggagttat ggaagaaatg caaacagcga ctgataagca atcagttaaa aaagctttaa
    19921 gtcgtgctta ttcatttatt gcagatactt tatttgaggg acaatttact ggtgaagatt
    19981 attgcaaagg aattgatgct cgtgagattg cctcattgac aggaaaatta ttgaagtctg
    20041 ttactgcagg ttttgatgaa acttatacag agacgaaaaa aaagtaagtg aggcgctcaa
    20101 gtcaccttca tttaagtatt cgattactta ccgagaatta gatataaaga cgcaattact
    20161 cgaagcaggt tggacgttac cagaaattga acgtactgac ttggatgagt taatgcgtct
    20221 ttttgctttt agagatgcgg taaaagagca tgaagatgtt gagtactatg ataatttcac
    20281 tcaattttag gaggtgatac tttgaataac gatgacctaa ttctgaagat gatactagat
    20341 gaatctggtt ttactgccgg tatgaataat gctgtcaaaa agttgaactc ttttgatgaa
    20401 acaatcgaaa gaaaaagtag aaatagtggc agttcattag gtagtatttg gaaaatattt
    20461 gctggaagtt ttctagctag cggagtaact agaatcgtag gagctggctt tgatctaatc
    20521 aagggttcca taagtggagc ggttgatcga gtagatacga tgaataacgc tctacgaaat
    20581 ttccaaaata tgggatttag taattcagaa attatgaaga atatcggaaa gaatgggctt
    20641 ttatctcaag gtattcaagg acttcctact gctttgaatg atgcgataag tcatgttcaa
    20701 cttcttgctt cttctacagg tgatttaact cgttcaactc aaatatttaa agctttaaat
    20761 gatggaattc ttggtttcgg tggttcaact gatcaagtta atgaagctgt tattcaatta
    20821 tctcaaagtt tctcaaacgg aaaagtagac gcacaaactt ggaactcaat gatcaacgct
    20881 caacttggac ctactctttc tgctattgct aaaaagatgg gaattacaat gggagatctg
    20941 aaagaaggtt tatctcaagg taagatttct gttgaagaat tccaaaatca attaatagaa
    21001 atggatacca aaggtggcgg aggacttaaa tcattaagtc aaatcgctaa agattctact
    21061 aagggaatta aaacctctat acaaaatgca aaaacagctg ttacacgtgg tgttggtgag
    21121 gtaatagaag gattaaataa agcattagta gattcagatt taggtggatt taagggaatt
    21181 attgataaag tgggcagttc aatggaatcg ttcttaaaag taattgctgc aaatattcct
    21241 acagcagtat catttttaag taatttattt aacgaaattc aaaaatttgg ttctgctttg
    21301 aaattcatga tgccctttct tgttcctgca gctaccgctt ttggagcgtt catgtttcaa
    21361 cttaaaggta taccagcaat aataaaaagc ttcaataatt ttaagaatgc cataattggc
    21421 gttggaaatt cattaaagat tatgggggca atagctgctg ctaatccatt tgttttaatt
    21481 attggtgcag tcgttggtgc aatagctgtt ttcggttact ttatggcaac caatgaagag
    21541 cttagaaaca aggttatatc tatttggaat gatgtgaaag attccgtaat tggtgtatta
    21601 aaaaatataa aggactgggg aattgatact tggaattctg ctaaagaaat ggcatcaaat
    21661 gcagttgagg gtgtcaaaga tgcttggtca ggaacaaagg aatggttttc taacacctgg
    21721 aaaggtatta aggatggggc cacaggttta tttgataaaa cagtagagac ttcaagaaat
    21781 gcagtcgata gtgtaaaaaa tgcatggtct aatatgaaga aatggttttc tgatacttgg
    21841 caaagtataa aagattcagc cagagaaaaa tggaatggaa tcaaaggttc tattatgtca
    21901 gttgctggtc cattgattac aggtataaag aatgcatttt tacatgttac tttttatcta
    21961 gagaccttat ggaataattt agttgagatt ggtaaaaacg tctttgagat tttaaaaaat
    22021 gttatacttg cgccggtttt gtttattacc tccatgattt ctggtggatg ggaagaaaca
    22081 aaaaataata tgattgctgt ttggaataat attaaagaaa gtgccataaa tatttgggaa
    22141 tctataaaga atatatttgt aagttatttt acaaatattt attttgctgc actcaatatt
    22201 tggacagggt tcaagcttac attgataaat atttggaatg aagtggtaaa tcaagctaaa
    22261 tcgatttggt taaacataaa gtattttttt attaatcttt ggattgatat aaaatatttt
    22321 gcaattcaga aatggattga attgaagttt tctattattc aaacttggat cgatttaaaa
    22381 tataatgcca ttactacttg gaataatatt aaacagtttt tcaaagatac ttggaaaaat
    22441 attaaagata cggcatacaa tacgtggatt tctataaaaa ataccatgat taatacatgg
    22501 aataacatta aggactcttt ctggaatatt gttactggaa ttgttaattc tgctgaaaat
    22561 gcatggacaa atctaaaaaa tggtgtttca aatgctatta atcgggtaaa agaaatcttt
    22621 aattcgttaa gggaagtcaa tttatttgaa attggcaaga acatcattga tggacttata
    22681 aatggtgtaa aagaaaagtg gaatgctttg aaagatacta tcaaaggtat tgcaggtaat
    22741 atcaaaaatt ggattaaagg agctttagat attcattctc catcaagatg gatgcgtgat
    22801 atggttggta aaaatattgt tcaaggtatt atcgttggta ttgataaaga gcaaagtaag
    22861 ttagatcaaa ccatgacaaa tttagtaaaa acaccgtctg ttcaacctgt agtaaatgga
    22921 ataagtcacc taccaattac acaaacaaag caaaatatat ctgctagtga aacaaaagaa
    22981 attcatttac atttaaatgt ttatggagat ttacctgatt caatggttcg gaagattacc
    23041 acaaaaatta aaacagaatt gacaagacaa atgaaacgag atgctggtgc agtaggagga
    23101 acattatatg caacttaaga gaggacaatt ttttattaat caacattatt cttctgaatt
    23161 taatgtgtat attcaaaaca ggcctgcttc tgtttcagct agtcgagtaa ttgaattgag
    23221 agagcgagaa ggaaatgatt ctatcattat tgataaaggc tattacaaaa atgtgactag
    23281 aaagattgag tgttactata aagcaccttc aattgatatg gtgcaggagt gggaagatcg
    23341 aatcactgaa tggttagata tgtcgtctta tagtgatttt attctttact atgatgaaca
    23401 atatatttat caagctgtag taatagaagg acctgaattt aaaggaacaa gaaaaacagg
    23461 aaatattgtc ccttttgaat ttacagtgag tattcgacca tttaaggaaa attacaatgg
    23521 tagatttact attcgacaaa cagaaacttt tgaaatttat aacccagaga agtattcttc
    23581 aaaaccgctt attaaattga gtggttctgg agatgcttct ttttatatta ataaggataa
    23641 atatgatttg aaatcgttgg acagagaatt atttatagat tctaaattag aagaggctta
    23701 tcgaaaatta gatggtaatt tagaacatca ggatcaggtc accttatttt tagattttcc
    23761 atttttatat cctggaaaaa atgaaattaa atggacgaaa aatattcatt catttgaaat
    23821 aatgcctagg tggtggagaa aagtatgaaa ccaagaatat atagtcctac tgaaacagat
    23881 ttctcaacga atggcttagg aattttaaag gattgtacaa gatgtggaat atatgaagta
    23941 gctaatggaa aatatgaatt agagttggat tatcctttag gaactagatt tgatgaatat
    24001 tttgaaaatg actatcaaat aaaagcaaag ccaaacgatc aagaagagta tcatatcttt
    24061 tttattgatg ataaagatat agatactttt ttaaatacag taactattta tgctcagagt
    24121 cgtacaaatc gacttggaag acgggtagtc actcttgtgg aagtggactc taaaactggt
    24181 caagaagcga tatcaattat tgaaactaaa atggataaaa aatctgacat acgactttat
    24241 tctgatatta cggctgtttc tagcacaatc tttgaagcga gaaatgtttt aaattgtatt
    24301 gctggtgaac aaggatcatt gcttcaatat tggggcggag aaattaaacg tgaaccattt
    24361 aagctttctt tgttaaagcg aagaggtcgc aataatgttg gaacgattcg gtatggaaaa
    24421 gatatgtctg gtttgaaggt caaattagat tggacaggta taaaaacaag aattattcca
    24481 tatgctgatc ctcaaagtga agtaggtacg actaatcgaa tttatggttc gccagtagat
    24541 agtgcctata ttaataacta tcctgatgtc tatacagaac atgttcagtt tacagaagaa
    24601 caaggagtaa aagacattaa gagcttaaat aaaatagcta aaaattactt taaaacgatc
    24661 aatcctggct gtgataagcc taaagtttct attacggttg aatttgataa gttgactgat
    24721 agtgaagaag cgaaagaatt tgcgaagatt agaaattatg gtctgtttga tacatttaaa
    24781 atatatcata aaaaatataa tatttatctt gaatcaaagg ttagtggact acaatacgat
    24841 tctttatctg aaaaaacttt gaaattagaa gcaggagaca ttcaagttgc tttttatcag
    24901 caacaagctg taactattca ggataaatta aaagattatg caacgaataa ttatatgagt
    24961 gattttaatg attatgtttc ttcaatgatt acaggtcaag gcagtgcagg aggatatgta
    25021 gttttatggc caaaagaaaa accttccaac atctttatta tggataatcc agatttagaa
    25081 aaggcaagag aagtactaag aatgaataaa aatgggattg ctttttctaa gaaaggatgg
    25141 cgggggcctt ttaattcagc ttggacatta gatagtatat ttaatgccaa ttttatacaa
    25201 actggattaa tcaaagcaga tatctttcaa aattctttta acaagactgg agatgtattg
    25261 aaattagtta atggactact ccaaatttgg aacaacaaaa agaaaataat ggagttaact
    25321 aaaaaaggaa tggaattttg gaattctaag ggttcaattg gaacgattgg aacaactgat
    25381 tctgttggta atccttttcc tggggcttct actccaaccc ctattgaaga taattcttta
    25441 gttattcgta caaatggaga cggcaaatat attttgattt ctcctaaagt aggaaaagga
    25501 ttagttttat taggaaatgg taaagcaatt tattttggag acttagatgt acaaggaaaa
    25561 cttacagtta aaggaaaaga aataattcct ggacaaggtg gtggtccatc gggaggtgga
    25621 gaatctactg gtgggtatcc tagtgaatta aaaacagatg cagaaaagag agcttggaga
    25681 at
//
LOCUS       DANKMN010000044        25264 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000044 DANKMN010000000
VERSION     DANKMN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(119..565)
                     /locus_tag="PW224_001693"
     CDS             complement(119..565)
                     /locus_tag="PW224_001693"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL2908382.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(576..2789)
                     /locus_tag="PW224_001694"
     CDS             complement(576..2789)
                     /locus_tag="PW224_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL2908383.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(3022..3786)
                     /locus_tag="PW224_001695"
     CDS             complement(3022..3786)
                     /locus_tag="PW224_001695"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL2908384.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(3787..4734)
                     /gene="prmA"
                     /locus_tag="PW224_001696"
     CDS             complement(3787..4734)
                     /gene="prmA"
                     /locus_tag="PW224_001696"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL2908385.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(4747..5235)
                     /locus_tag="PW224_001697"
     CDS             complement(4747..5235)
                     /locus_tag="PW224_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL2908386.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(5201..5872)
                     /locus_tag="PW224_001698"
     CDS             complement(5201..5872)
                     /locus_tag="PW224_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL2908387.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6010..7296
                     /locus_tag="PW224_001699"
     CDS             6010..7296
                     /locus_tag="PW224_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL2908388.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7368..7562
                     /gene="ssrS"
                     /locus_tag="PW224_001700"
     ncRNA           7368..7562
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PW224_001700"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7640..7924
                     /locus_tag="PW224_001701"
     CDS             7640..7924
                     /locus_tag="PW224_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908389.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            7997..8476
                     /locus_tag="PW224_001702"
     CDS             7997..8476
                     /locus_tag="PW224_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2908390.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            8699..8842
                     /locus_tag="PW224_001703"
     CDS             8699..8842
                     /locus_tag="PW224_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL2908391.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            9420..11003
                     /locus_tag="PW224_001704"
     CDS             9420..11003
                     /locus_tag="PW224_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2908392.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(11440..12624)
                     /locus_tag="PW224_001705"
     CDS             complement(11440..12624)
                     /locus_tag="PW224_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL2908393.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(12649..13656)
                     /locus_tag="PW224_001706"
     CDS             complement(12649..13656)
                     /locus_tag="PW224_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2908394.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(13828..14187)
                     /locus_tag="PW224_001707"
     CDS             complement(13828..14187)
                     /locus_tag="PW224_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908395.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(14159..14530)
                     /locus_tag="PW224_001708"
     CDS             complement(14159..14530)
                     /locus_tag="PW224_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL2908396.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(14547..14849)
                     /locus_tag="PW224_001709"
     CDS             complement(14547..14849)
                     /locus_tag="PW224_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908397.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(14866..15336)
                     /locus_tag="PW224_001710"
     CDS             complement(14866..15336)
                     /locus_tag="PW224_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL2908398.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(15311..15589)
                     /locus_tag="PW224_001711"
     CDS             complement(15311..15589)
                     /locus_tag="PW224_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL2908399.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(15586..16635)
                     /locus_tag="PW224_001712"
     CDS             complement(15586..16635)
                     /locus_tag="PW224_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL2908400.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(16592..17521)
                     /gene="tadA"
                     /locus_tag="PW224_001713"
     CDS             complement(16592..17521)
                     /gene="tadA"
                     /locus_tag="PW224_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL2908401.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            17653..19407
                     /gene="ade"
                     /locus_tag="PW224_001714"
     CDS             17653..19407
                     /gene="ade"
                     /locus_tag="PW224_001714"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL2908402.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(19505..20167)
                     /gene="trmB"
                     /locus_tag="PW224_001715"
     CDS             complement(19505..20167)
                     /gene="trmB"
                     /locus_tag="PW224_001715"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL2908403.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(20237..21019)
                     /locus_tag="PW224_001716"
     CDS             complement(20237..21019)
                     /locus_tag="PW224_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL2908404.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(21223..22434)
                     /locus_tag="PW224_001717"
     CDS             complement(21223..22434)
                     /locus_tag="PW224_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908405.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(22427..23164)
                     /locus_tag="PW224_001718"
     CDS             complement(22427..23164)
                     /locus_tag="PW224_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2908406.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            23302..23736
                     /locus_tag="PW224_001719"
     CDS             23302..23736
                     /locus_tag="PW224_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL2908407.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            23729..24073
                     /locus_tag="PW224_001720"
     CDS             23729..24073
                     /locus_tag="PW224_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2908408.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            24253..25263
                     /locus_tag="PW224_001721"
     CDS             24253..25263
                     /locus_tag="PW224_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2908409.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         7642 a         5264 c         4504 g         7854 t
ORIGIN      
        1 ttccgactgc tgacaaaaga agcgcttttt gcagacaaaa aagccactta agttagagtt
       61 tatcagtcta acttaagcga cgtgtttcat agtttttact agtgaaaacc agattacttt
      121 atttgttttt agaatcaata atgatcgtta caggtccatc attgacgaga gaaaccgaca
      181 tatctgctcc gaattgtccg gtagcgactg tgatgccgcg acttctgatt ccttcattga
      241 atgattcgta taaaggaata gctgtttctg gtcgtgcggc ttccacaaag cttggtcgat
      301 ttcctttttt cgtatccgca aacagtgtga attgagagat acttaaaatt tgtcctccca
      361 catcttctac actaatattc atttttcctt gttcatcttc aaaaatacgc atttgtgcaa
      421 ttttttttac cagatagtcc acatcttttt gcgtatcggt ttcatgaatg cctaataaaa
      481 caagtaggcc gtagccaatt tttccaattt cttgctgatc gactgacaca gcagctttac
      541 ttacccgttg aacaactgct ctcatatctg tcctcctatc cattcgtccg gcgtacactg
      601 tagacatcag gtactgcttt gattttatcc acgatactct ttaggtggga taaattctgg
      661 ataccaaccg tcaaatggat cgtcgccatt ttatctttat tcgtccgtgc ttcaacgctg
      721 ttgagatttt tcgttagcgc attgactgtt tgaagtacat cattcaataa gcctgaacga
      781 ttatagccgt aaatttccaa atctgcatca tactcttttc gtgtattcga tgtatcttcc
      841 cattcgactt caatcaaacg ttctgctaca tttggtttgt caggctgaac attcggacaa
      901 tctcgacgat gaatggatat ccctctgcct ttagtgatat aaccaacgat atcatcacca
      961 ggaatcggat tacagcagcg actgatacga attagtaagt tttctacccc ttgaatgaca
     1021 acgccacctt catgacgtac tttcattttg tcaggttctt ttttcggctg attcatgatt
     1081 tcttctgctt cttgcttttg ctgctcgatt ttttgttctt tacgttcttt ttcagtcaga
     1141 cgattggcca tcgtcaaggg gctaacttct ccatacccta cagctgcata gagatcatct
     1201 tctgtttgat aattaaaacg atcgagtgct tcttgcagtt tgttcttcgt caaaatatct
     1261 ttaggcgtaa atcctagatc tgtaatacat ttgaccacgg attcgtggcc tttgatcaca
     1321 ttttcttctc gatcttgggc tttgaagaaa cgtttgatct tatttcttgc tttgctagta
     1381 gcaactaatt tcaaccagtc gcgacttggg ccaaatgaat ttggagaagt catgatctca
     1441 atgatatctc cgtttttcaa tttgtaatca agctgcacca ttttgccatt tacttttgca
     1501 ccagtggttt tgttaccgat atctgtatgg atactgtatg caaagtccaa tggtccggat
     1561 ccttttggta actcagtaac gtctcctttt ggtgtgaaga cgtagacttt atcactaaag
     1621 atatctcctt ttacgccttc cataaattca gaagcatcat agctttcgtc ttgaagttcg
     1681 agtatctcat ggaaccagct taattgtttc gtcataccat ctggttctac tttttcattt
     1741 tttccttctt tataggccca gtgtgcagca accccgaatt cagcgatttc atgcatttcc
     1801 tgtgttcgga tttgaatttc taccggattt cctgccggac cgatcacggt agtatgcaaa
     1861 gattggtaca tattagcttt tggcatggca atataatcct taaatctgcc gggcattggt
     1921 ttccattttg tgtggattgc tcctaataca gcataacaat ccttgattga atctacgatc
     1981 actcgaatag ctagcaagtc ataaatttcg ttgaactgct ttttctgatc tttcatttta
     2041 cgatagattg aataaatgtg ttttggccgt ccatagattt ctgcaaaaat ccccagctct
     2101 tctgtcgcta ttcgaatatc ttcaacagta ccacttacgt atttttcacg ttcttctctc
     2161 ttcgtctgca tcaagtggac gatacggtaa tactgttttg gatttagata acgaagtgct
     2221 gtatcttcta attcccattt gatccggctg atccctagac gatgtgcaag aggcgcatag
     2281 atttctaaag tttcctgagc gatccttctt tgcttatctt ctcgcaggtg cttcaacgta
     2341 cgcatgttat gtagtctatc agctaatttg accatgataa ctcgtaaatc ttgagccatt
     2401 gctagcagca tctttcggtg gttttctgct agctgctctt cgtgggattt atattttatt
     2461 ttccctaatt tggttacgcc gtccactaac atagcaacgt catcgccaaa ttcttctctc
     2521 agatcttcta aagtgatttc tgtatcttca acaacatcat gtaagaagcc tgtagccact
     2581 gtatggggat ccatttttaa atctgctaat atacctgcga cttggatagg atgaataatg
     2641 tagggttcgc ctgattttct aaactgtccg tcatgtgcag cagtcgcgta ttcgcatgct
     2701 ttctcaacaa atgctacatg ttcgggaccc atgtattgac taacgagctt tatgacaccg
     2761 ggtcccgtta aaatttcttc ttttgtcaca ggaatcgatc cccctttctt ttttcggtaa
     2821 aacgggaatc gggacaaata tctgtttgat aattgtccca aaatccgtgc agttatacgt
     2881 ttttttacgg aaatgttcgc tttttaaaag ctttttcaaa cgatttttcg tatccattgt
     2941 ataaataatt gtttctatta tacaaattgt tgactatttt ttcaattctt tctgtcgata
     3001 ctcttttatt aatgaagaac gttagctgaa agaaggattc attaattccc attgatagct
     3061 agcagcactt aataaataaa gaggtgctgt ttctgtacga aggattcttg gtcctaatcc
     3121 acaaagtatc cctttgtgtt ctacaaaata atcgacttca tcgcgggata atccaccttc
     3181 tggtccaaag acagctaaga ttcgactatc tggttttgtt tcactaagaa ttttggctgc
     3241 atttgatttt tcgccttgct ttgctgattc ctcataagca accaagacca cgtcataatc
     3301 atctagccgc tcaaataaat cttgtttcgt ttccaataac tctactgatg gcacgatctg
     3361 ccgatgggat tgttccgctg cctcttgagc aatcttttca agccgttgtt gtcgtttcat
     3421 acgttttttt gcatcccatt taacgacaga ggctgatgcc gggaaaccaa gaaaagcttg
     3481 cgctcctaat tcagtggatt tctgaacgat ccaatccagc ttttcccctt taggatagcc
     3541 actcgctatg gtaatcgaac ttggcagttc cttttgattt tcttcttttt taatttcttt
     3601 cagataaaca gcctcttcag taatctccgt gatttctgca acaatggcta tttgatcatt
     3661 gaatgccaaa aaaacttcat cctcgggttc catcctcata acccgtacaa tgtggtgata
     3721 gttttcccct tctaaaacaa aatagtcttt tgattcatat gattctttta aaaaataccg
     3781 ctgcatctat tccacctctg gttttttcaa aataattgca taccagtctt tttgctggaa
     3841 aatttgatcc acgataaacc cttgttctaa catggcatcc agaaccattt gcttcttctc
     3901 ttcaatgatt cctgaaacga tcagcgtgcc tgtttgcttc agtaaacgcc aagcatctgg
     3961 gatcatcaat aagatgatat ctgctaaaat gttcgcaacg attacatcac tttcaatctc
     4021 gattcctgtc aaaagatcgt tagctgatac atggacatct ttggctattt cattcatatc
     4081 catattttct ttcgccgaac gaacggctac ttcgtctaaa tcaaaggcat agacatcttt
     4141 tgcaccaaga tattttgctg caatacttag cacgcccgac ccagttccca catctagcaa
     4201 tgtttcacca ccacgcaaga ctgtttctag tgctgtcaat gtcaaggaag tcgtgggatg
     4261 agtgcctgtg ccaaatgcca taccaggatc tagtgtgata attttttcta gaggatcttc
     4321 tgtctggtag ttttcccagc ttggcgcaac cgtcagcatt cgcgtaatac gcaccggatg
     4381 atagtatttc ttccaggctg ttgcccagtt gctttcctct acttcactaa ctgttacttt
     4441 attttttcca atagctagac caaactcagg taattgtgtg atacgatctt tgataaaagg
     4501 caagatttcc ggtaagaaag tcgtttccgg aaaataagcc atgactagtg cgccttcttt
     4561 cagcgatgta aaatcatttt tatctaaaat ctcaccatat tgatccgact gaaaatgttc
     4621 aacatctaaa gaatcttcaa tcgcgacacc actcgcccct gcctccatca aaatattagc
     4681 aactgcttcg actgcttcac tttcagtttc tacttttact tcattccatt tcatatctgt
     4741 cactccttaa taacttgggt acggtaaata agtatctgct tcattttgct gttttacttc
     4801 tactaattct gctagttgct gattatccca gtgaagctcg aatacagctt cgtcatcgtc
     4861 ttcatccaaa aaatcaagca aatcgctttg cccttgagct aatacttcat ttagatattc
     4921 cactaaagaa tcagctactg attttgccaa cccttttttt ccttcataag ggattactgc
     4981 tagataattg cgttcatcaa aaacagattt ctgaggatta aaaagaagta cgccgtcttc
     5041 aaattcgata attggttctt gcgaaacgat cccttctgca tcatcaatct caaattgtgc
     5101 tttattttct gcaaacaagc gaacaacgat ctcgatcgta tgattttttg tatcccaatc
     5161 taaagctaca tcatagtctg tcacttgttt ttcaattatt tcatctaaat atgttaatag
     5221 atccttcttt ttcattttct ttcctccaac cccaatcttt ctcgatttgg ttttttcttt
     5281 gtaaagagat ataaggattt cccgacacaa caaaacgcaa cggcttttct gtccatacac
     5341 ctttgtttgg gataccgatt ctaggcaaag cattgatctt ttttggtgtc ttttttttct
     5401 caaaaactaa gcgtaacgaa ctatctgcta tagactgacc ataaagttct tgaggaattg
     5461 ctaatgcttc cactaatttt cccggaccgt tgctgatatc tggaccagtt tttccgccac
     5521 gatttttact catttgatca atcattgcgg cgggttcgat tgcacgtatc attactcctt
     5581 gaggaatccc ttctggctga gtaatgatgt tcaaaatccg atgagtatgc atcgtgtaaa
     5641 ggtagatcgt acctggtttt tcatacattg cccgcacacg cggtgttctt cttaaaccat
     5701 agctatgggc ggcctcgtct tctggaccta ggtatgcttc cgcgtctaca atatatccag
     5761 ctaatcttcc ttctggtgtg tcatgctcca aatacatccc caataaatcc cgtgccactt
     5821 ctggcgtcgt tttattccaa aaaaattcat ctatcttttg tttatccgtc atttttctca
     5881 cctactttat catacacaat attagttgga taagctatga aaacaaaaca acagaaggct
     5941 gttctttcta ggcatcttgc taaatatgat ataattaagg gacgtaaaaa caagaaggag
     6001 gaaaatccta tgcaacagcc attggcttat cgcatgagac ctcgaacgat cgatgaagtc
     6061 gttggtcagc agcatctagt cggggaagga aaaatcatac gccgcatggt cgatgctcgc
     6121 atgctctctt ccatgattct atatggacct cctggtactg gaaaaacgag tatcgcaagt
     6181 gcgatcgccg gctctactaa ttatgcattt cgcatgctaa acgccgctac agattcaaaa
     6241 aaggatctac aagttgtagc ggaagaagca aaaatgagtg gtacagtgat cctcttattg
     6301 gatgaagtac accgattaga taaaacaaaa caggattttc tgcttcctca tttagaaagt
     6361 ggccgaatca tcttgatcgg agcgactact gagaatcctt atattaccat caatccagcg
     6421 atccgcagcc gtacacagat ctttgaagtc aaaccattaa cagaacaaga catccagttg
     6481 gcagtcgaac atgctttaag ggataaagaa cgcggccttg gacaacaagc aatccagtta
     6541 gacgaagatg ctcttcttca cctttcgcgt gcaacgaacg gcgatttgcg gagtgcattg
     6601 aatgggctgg aactggctac actatcgact ccttcagata aagaaggaag gattcatctt
     6661 acactgtcga tcattgaaga atgtgtccaa agaaaagctc ttacccatga caaaaatgga
     6721 gatgctcatt atgacgttat ttcggctttt caaaaatcga ttcgtggaag tgatgtggat
     6781 gctgcacttc attatctcgc ccgtttagtt gaggcaggtg atctggcaag tatttgccgg
     6841 cgtctgatgg ttatcggtta tgaagatata ggtttaggta atcctgctgc tgcggctcgt
     6901 acagtgaacg ctgtattagc tgctgaaaaa ttgggattgc ctgaggcacg tatcccacta
     6961 gcagacgtcg tcgtcgatct ttgcttgtcg ccaaaatcca attctgccta catggcatta
     7021 gatgcagcac ttgctgatat tcgagagggc aaagctggag atgttcctga tcatttgaga
     7081 gatagccatt acaaaggtgc gaaatcactc aaccgtggcg tgggttatca gtatcctcat
     7141 cattttgatc aggcttgggt caatcagcaa tatttaccag ataaactgaa gaatgctcag
     7201 tattatcagc caaaagatac agggaaatat gaacaggctc tgggtcaaca gtattaccgt
     7261 ataaaagagt ggaaaaagca tcctccaaaa aactagacaa gaaaacggtt ctgtttctgc
     7321 gagctctctc attgctcttt ttaaattcat atgctactat agaggtggga aatctgtgat
     7381 gttcgaattg tcctctttgt gtgttgaccg aacattttta ttgatatctt gggacccgtc
     7441 ttgtgtcact gctggtaaca tgccttatca tttggtagtt cgaagctacg accatgggac
     7501 ccacctgctt gaattcgcgg gatcaatact acaggacaaa caacggcatt aacggagatt
     7561 accatctttt aaagaataaa gcaatttgct cttggcaaat tgctttttct atttgttgct
     7621 tattttggag gatttgttca tgattaggat tttaatgatt atcgccactt tagtattatt
     7681 attcgttagc tattatttat ttaataaaca ggatatcttt tttgtattga tcaacaaaaa
     7741 tgataaaaat caaggattcc tacaatttta cggagctgca tatgctgttt taggcgtaat
     7801 gggtatttta acagcttttt tcaatcaacg ctttattgcc ttggtctttt tactgatcgt
     7861 aattattgtt tctgccacct tcagtatccg atttgcaaaa aaaattgctg aaccaaagca
     7921 gtaaataatt ctctttttcc ctaaagtgtt ctacaataaa agagtacaat acgattgact
     7981 tgaggagtga atgcttatgt tacaacaata caaaaaaatc atggttgccg tagatggttc
     8041 tgatgaagct gaattagctt ttaaaaaggc ggtcaatgta gctatccgaa ataacggaga
     8101 acttctatta gcacatgtca ttgacacacg ttctttccaa accgtttcat cctttgatgg
     8161 aatgcttgca gaacaagcta ctgaaatggc aaagcaaaca ttggcagatt atgaaagcaa
     8221 tgcgaagaaa gctggcttga ataatgtaac ctctgtcgta gaatatggct caccaaaaca
     8281 aattattgcc agagaaatcc cagaagataa tcaagtagat ttgattatgt taggagccac
     8341 aggattgaat gctgtagaac gtttatttat tggttctgtt tctgaatatg ttattagaaa
     8401 cgcagcttgc gatgtgcttg ttgtccgaac tgatttagaa aaccaattgc cagctaaaga
     8461 ccacaccaaa gcttaacacg tagacgacct gccaactatc ggcaggtttt tttgcacaaa
     8521 aaaccgtgat aaaaacaaaa gacgcggcaa gcactcatcg gtctttataa tgtttaaaat
     8581 aaagggaaaa cagtcaatga gttcaagtaa aataaaaaaa agtgaacacc agggcgatta
     8641 gtcgccctgg tgtggtttaa aacgacctaa gcatgccaaa gcttattggt tcgtcaatgt
     8701 ggaagtttcc gatgaatctg atacgaaaaa atatgaagta tcgatggcgg ataccgattt
     8761 tatcgccaaa ctaaaatttt atttcgggtt cgggtaccgc gagaaggcaa gtgctacatt
     8821 cggtaaatca ttcaagaatt gatgaaaggc taccgcttgc cattgcaacc acaacgatac
     8881 tcctgaacgc gatgttggtg agatttccaa agagaagagc tgtttaatgc aacggtatta
     8941 agcaaatggc aaaaggaagt catgtagaca aagtttttta tcaaacacca tatgatttcg
     9001 cctgaacgct ggtttagctc agaatacaat gatgtggtcc acgagacatt accctcagtc
     9061 agataaaaaa agctctctga cagaattaca catcgataaa tattaagtaa atgtcagcgc
     9121 tagttgaact ttctgacact ttcaggtaca ctggaaaaga caactacacg aaggagggaa
     9181 aatactatga tgcgcatgac taaaataaca agtaatgaaa attaccaacg ataccatatc
     9241 gcatttagcc atgcaattgg ttttacagtt gctgttgacg ggacaagttt gtccaaaaca
     9301 gttgaattca cgtaaaatca atggccatct caaggtattt ttctcatgat aatctgagtt
     9361 taaataacac aggccatgga taaccatggc ctttttgagt ggtctcaagg agagagatca
     9421 tgatcataca agcaatttct attaaaaaaa caatcggcgg gaatccatta tttgaggatt
     9481 tatcctgtca aattgaagct ggtgaaaaaa ttgtcttagt cggtatcaac ggaactggca
     9541 aatcaacctt tttacaaatc ttgaccggca gagaaggtgt cgacagcggt gttatcagtc
     9601 gcaaaaaagg cttaaagatc gggacggtcg aacaagaact tacagtttcc gaagcgaccg
     9661 tcaaccacta cttgctccat tctgccgctg agatacagga cctcaaacag caaatgagtc
     9721 gctacgaagc attgatgaca gaaccagata caaacttaga gaagtgtctg gcccgttacg
     9781 gtgagctgca gcatcgcttt gaagaactag gcggttacgt attagaagac cggattgcag
     9841 cgatcttgca aggcttaggg attccacacc gtcaggaagc acggttaaca gaacttagcg
     9901 gaggcgaacg agttaaagtc gctttagcca aaattttagc agccgatgcc gatttacttt
     9961 tactcgatga accgaccaat catttagatc ttaacagtat tcgctggttg gaaaactatt
    10021 tacagaatac aaaaaaatcg gttctggtcg tttctcatga tcggcaattt ctcgatcaag
    10081 tcaccacaaa aacatgggaa ctggaagaag gtgcattgat tgagtatccc ggaaactaca
    10141 gccgttttcg agtcttgaaa gaagctcgct tggctgagct gacaaaaaat tacgaactgc
    10201 agcaaaaaga agtccagcgg ttgaaagtca tgattcgtcg ttttcgtcag tgggcacatg
    10261 aaggagacaa tgagtccttc ttcaaaaaag ccaaagaact ggaacgccgc ctggcgaaac
    10321 tgacactcgt caaaccgcca cctcccccga aaaatcggct gcaaagcttg tctaatggtg
    10381 gaaaatctgg caaagaagtt ttcatcattc aaaacttgca ccagcagtac gctgatcaag
    10441 tactattcaa agacagcagt tttgcagtct atcgtggaga tcatttagcc atcatcggcg
    10501 acaatggtgc aggaaaatca accttactca aattattact cggtgaggaa aagccttacg
    10561 gtggcaccat caaattaggt agtagcttgc aaatcggtta tttgccgcag cagctgcagt
    10621 ttgccgatcc cgatagccgt ttgctggctt acgccatcac attgactgga aacgaagaag
    10681 tcgctcgacg gcaattagcc aaaagtggct tttatcaaag cgatgtgggt aaacggatca
    10741 aagatttatc cggtggtgaa aaaatccgtt tggcgttgct gaaactcttt ttagaaaaaa
    10801 tcaacgtcct gattttggat gaaccaacca atcacctcga cagctatgct cgagaagaaa
    10861 ttgaagaaat gctgcaagac tatcaaggaa cgctcctagc agtcagccat gaccgctact
    10921 ttttgcagca acacttccaa gaagcactcg ttattgatca ggagcagatc agccgccagc
    10981 cgctcggcca actgattgac taggacgttt tttcgcaaca aaaaacttga ttttcatacc
    11041 tgtgctttca cgattcaggc aaagtacagg tggaaaatca agtttttatt attcgatcaa
    11101 tgaatgtttc ggatcggttt tctagctaaa cataaagcga agaataaagt cgacagccca
    11161 cgaggctgat ctaaaaatgt tttttctttt tacatatgta atgtccctta taaagatgaa
    11221 tgaaaataaa atattgggat tttcctgaaa atcaataaat aaaatgccag tcttttccct
    11281 tttgaccact tttccaagca aaattcccta agaagatcct gtaaaaaaga agggaaattg
    11341 aagttaaatt accattttgc ggtcttctcc gcaaaaaaaa cacatgcaag aattccctct
    11401 tgcatgcgtt tcatctttta tcctaacgaa tgctttggct tagccgcgca gacgctccac
    11461 atcacgggca atcattagtt cctcatctgt tgggatcact aatactctta cttttgattc
    11521 ttttgtagag atatctactt cttcaccacg caattcattc aacgctggat cgatttcaca
    11581 accaaaccaa gacatacctt tgatgatttc actacgtaca tgtccgtcat tctcgccgat
    11641 accagcagta aagacaattg catcaacacc attcatcgtt gtcacataac cgccgatgta
    11701 ttttcggatg cggtccacga aaatgtcata agctaaacga atatgttctt tttcatagtt
    11761 cttttcaaga tcacgcatat cactagacaa tcctgttagg cctagcagac cagatttctt
    11821 gttcaaaaca ttgatcattt catcaatatc aatctctaat ttttccatta aatatgggag
    11881 aacagctgga tcgatatcac ctgaacgtgt acccattgtc accccagcta atggtgtgaa
    11941 tcccatagaa gtatccatgg atttacctcc atcgactgct gtgatcgata caccatttcc
    12001 taagtggcaa gtgatgattt ttagttcttc gatcggtttg ttcaataatt ttgccgcacg
    12061 ttccgacaca taacgatgac ttgtgccatg gaagccatat tttcttactt tgaaatcttc
    12121 ataataagac accggtaagc tatataaata attttgcttt ggcattgttg agtggaaaga
    12181 tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc tttttaaacg ctttgatccc
    12241 cattaagttt gcaggattat gcaaaggagc aaattctgct aattcagcaa tgcggtccat
    12301 tactttctca tcgatcacta ctgaatcccc ataagtttct cctccgtgaa caacacggtg
    12361 ccctactccg gtgatttcat cataagagct taaaatttgc agttcaatca actgatctaa
    12421 cagcattttt actgctactt cgtggttttc gatatccatg atttgttcga atttttgatt
    12481 atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg atacgttcga cgatcccttt
    12541 cgcaactacg gtttcttctg gcatttgata taattgccat ttcaaacttg aactcccagc
    12601 attgattgca attgttttag acatatgact ctccttctcg ttgaatgatt acagagcaga
    12661 tgatttccag tttttaaact cgttgaaaaa ttccgtcacc ttctctggtt cttttaatga
    12721 agcaagtttc acaagcaaga cttcttttac ttgttgtgct tgtggtcctt ttttctgtaa
    12781 gatcaaaata cttttttgcg attgtttatt acggaacaat tcatctggca attgaatcat
    12841 tccttgaaga taaccttctt ctttaaacca ttttttgatt tcttcacttt gatccgtttc
    12901 taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc ggcttcacat atttcattga
    12961 ttgctctagc aataaatgat gtgcataact atggccttct tcggtacttg ttaaaaactc
    13021 ttttgctttt tcgtcattcg gataataacc gattggcaaa tcgctgattg caaaatcgac
    13081 aggatctatt aataattcct gcaacccgtc ttgatgaaag tattgaacat ttgcttgtgt
    13141 caaatcactt gtagaagctg cgacagccaa caatgtatca tcaatatcca caccaattcc
    13201 ttctgtttgg taccccgcat tcgataggtt taaaagaact gttaataaca agttgcccat
    13261 tcctgccgca atatccagaa tcttaaccgg tgctttttta tttgtgtata gttgttcaat
    13321 aagaaaaaca aataaaaaac cgatactatc aggagtgagt tgatggttcg cctgcaaatg
    13381 ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt ctgcgccatt cttctggctc
    13441 gaaagaaagt ttcttcaact cttcataaag agctgtgatc cgttgcgttg tctcttttgt
    13501 cggcactcca tccaccacac gtacttgata atcatctatt aaattttctg cattttcgac
    13561 ataggcttct aaaaaggaag ttcccaatgc attctgtaaa agctgaatag cttccaggtt
    13621 ttgattaaac ccttgttcca ttttttctgg taacatggtg cacctcattt ctagatatat
    13681 tataacaatt ttttcacctg tttaattcta tcgaacaatt gacaaaattt caatcgtttt
    13741 cactgttttt tatttgtgaa acggcaaaca tttttttatt aaaaacagtg ttataacaga
    13801 aaagagggcg ttttcacgaa cattccatca ttctgtacta gtatctgtat cattttttac
    13861 ttcttctgtg aaccgaaaga taaacggtga gacttccacc tctaagaata gggactgttg
    13921 tacccactga tatgttacag atccagttgt gtagtaaaaa actccttccg aactttcggc
    13981 aagcgtagac tgttccgata aagccatctg cttcattatt tttgctatat aataattttt
    14041 gattcgtata ttaagatcca ttgacaaacg gtagctttct aatgctgcca tcaataaaaa
    14101 cgtacataaa agaaataaaa gcaagacaga aaataaaata gagcccttat aacattttct
    14161 actgatcgtt ttttgtccat atggcatatc catcttctcc attttcaaag tgtacataaa
    14221 aagaaattgt gtgttctttt tccaataatt gaagctcagt gatacttgtt aacataggtt
    14281 gatgtccgcc ggaagttctt cttttacgta tcattgcctg atatctttcg atgatatact
    14341 gtttatcctc gatcgtgtag tgcagttgct gatttttcac agagacattg tgactgctct
    14401 tgagttcttc ttctaattgt gctaaaaata ttagccactc tttttgatct ttacgctgca
    14461 aaaattcact tacttggatg gtttgctgaa tcaaaagatt gaatgtgagc aatagactgc
    14521 tcaatatcat cagtccaatt agacattcaa tcagcgtaaa tgctggaatt acttgttttt
    14581 tcaatactga tcgcttcttt ctccatattg acaaacactt tttcgattac tccattatta
    14641 tcgacaattt ggatatcgta tgggatcatt tcttcccttc gctctggtct tttgtgatac
    14701 atccgctgcg tcttgatatc ttcataaatc attcgaataa atagcagttc ctccccactt
    14761 ttcttttctg cctgaagcaa aaacagctgg ccaaagctca ctaagtagac acagacagaa
    14821 gcgatcataa gcgctgtaag ggactccaac aaaacaaatc ctgttttatt caattctttt
    14881 tgtatagcga ccactcccta tctggaattg atacgtaatc gtccgctctt tttcttccca
    14941 ataaaattga taagctttta gtgaactttc attcccggtt ctagcagcaa aagtaataga
    15001 agcatggcgt ttcaaggtaa tctcatcagg aatatctaat acttcccaat ttattttatc
    15061 tggattcgga acatgaaaaa tgatttgatt ctcttcacta ttccaataaa agcctgtttg
    15121 gatctgattc acaatagcga ttttttgcgt agaatagatt cgtttttcaa attggccgaa
    15181 aaattgatag actgccaatt cattcttcca tgcagaaaat gcgagaactg gaaaagaaag
    15241 gatcgttgta agtaataaaa tcagtaagag tgattcaaac aacgtatatc ctgatgctaa
    15301 aaccttacct tcaaggccgc tgatagattt ctgcatgttc tcgtttgata tagccttcat
    15361 tcaacaattc ctccacagta ggaactcttc cattattttg aagcgcatag agctcttttt
    15421 gtgtgtctac caactggatg atcgcttcac gactttcttg gtctacatga ttcctataac
    15481 gcgacaaatt agggacgaac aacaacacta atacactgat gatcagtaag acgattagca
    15541 tttcgatcaa tgtaaaaccg ctataataag atatttttct ctttttcatg gcaaaacctc
    15601 ctctatgctt ccgtaaacag gtaataaaac agcggcataa actaataaaa tcaaagccgc
    15661 tacgcccaaa aaaatgattg gctgaataaa atgcatccag tgatgaagac gtttgaaaag
    15721 ctgttttctt gtccattcgc tgtaaaaaat cagttcttta cctaaacttc cctttgcctc
    15781 tccctgtaaa acaattttac tgaattcttc tgatagaaaa cgataattct ggaactgctc
    15841 agccaatgac atacctgcct ccaatccttg aatcaactta gaggcaagat gctgaatcag
    15901 actttcttgt ttcgtttcct ttaagcagac tatgatttgc cttaattcga aaccttcatg
    15961 aaaaagcttt ccaatctcta gtgacaaata agccgattga tacaaggaat atatcggtcc
    16021 aatcaataat tgtctgctta tccattcact ctgatcaaca ggagacaact tcatcagacg
    16081 ataccttact agtaaagcca atagagtaac cgctaggatg aatagtagaa cataccaatg
    16141 aaagacttgt atcagctgaa taccccaatg ctcttgcatg accattcctg aaccaataag
    16201 ctgaggcaaa acgaacaacc gcatactaaa caagatacct agcaaaaagg tgagcaatag
    16261 catcggatat gcaagcagct ttttcaaatc attgcgaaat ccttcaacaa gccgaaactg
    16321 tttggcaatt tccaacaaag ttgtcggcag attcccatga cgttcagcta actcgatctg
    16381 taccagttgc tctgctccat atcctatttg cgaaagtacc tcactaaacg cttgcccttc
    16441 ctttagctga tgttggatat ttgccaaaat aacaggtgga aacgaacgga tcgtgagtaa
    16501 taaataaagg gattcttgca gactaaaacc agataaaagc agttctccca tggtttgtgc
    16561 aaactggttt tgctggcttc ttgttaatcc tttagaatag ttctgactgc caaatatttt
    16621 cctgagcttc ttcataactt tcctcccatg atttttttac tgattcttcc tccataaatt
    16681 gataactcca taaaacagaa ggatggtgat tggcatcttc caaaagttct tgataaatga
    16741 caccagctaa acattcagat agctcttctt ttttctttgt cagttcatgg attctgacca
    16801 atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac ccggtgccct gttagtgctg
    16861 ctcttaccgc agcatgtgca gttgattcat ctcttatctc gccaatgatt agcaagtctg
    16921 gacgatgccg taatgcgagt ctcaaaagtg tatcataagt ctgtgctatt ttcggattga
    16981 tctgtagctg cagaaaaaac ggttcttcta tctctacagg atcttcgata gtaatcactt
    17041 gcaatttttc ttccaaagcc aaccgataca ttagagaagt cttgcctgat ccgactggtc
    17101 cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa aatagttgca aaatctcgag
    17161 aactaaagca acgatggggt gtttcaaaag taccgtataa tagccgaatc actaaactct
    17221 ctcgttgaag gtaatctcct acagaagaca accgcagacg aacttcctgt ttttctaaag
    17281 aataagtaaa tgcacctaac tgagcttttc ttttttctcc gatatccatc tgtcccaaat
    17341 acttaaatct tgagatcagt tgctgtccat ctgccgaaga gactttcttc cagaatgttc
    17401 gtttttttcc atagcgaaaa tagatgtaaa atacttctga agctggcaat atatatacgt
    17461 cttgggcatg tttggcaaaa ctttcactta tcatttgatc cgataatcgt tttatttcca
    17521 ttattctccc ctccacaaaa aacatacgtc tttttgatct ttttgtttca aataaaatga
    17581 atttacaaaa aaatattggc aaaaaccgaa tttatgttat acttataaaa atttatcttg
    17641 ggaggcactc cattggaaac aacattaagg aaatatatcg aagtagcaag taaaaaaaag
    17701 cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg tctttacgaa agaaatcatg
    17761 gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa tcggtgaata cgaaggaaaa
    17821 agcgtacata atgctaatgg gcaatatcta gtgccaggat tcatcgatgg ccatgtgcat
    17881 atcgaaagct cacttttatc accaagcgaa ttttcaaaag tatccttgct tcatggagtt
    17941 actactgtag tgacagatcc ccatgaaata ggaaacattg ctgggagtac aggtcttgag
    18001 tttatgatag aagatgcaag acaaacactc atgaatatct tttttatgct accttcttgt
    18061 gtacctgtaa ccccttttga gaccaatggt gcaaatttaa atgcaaaaaa attagcccct
    18121 tttttacaaa aacccgaagt tttaggctta gctgaagtca tgaactatca agcagtagca
    18181 aataatgaaa cagacatcct agaaaagatc caattgatgc accagcataa gaaaaaaatt
    18241 gacgggcatg cagctggtat cggcatggaa gagttaaatg tttatcctgc agcaggtatt
    18301 cggacggatc atgaagctac aactgcaaaa gaagctaaag aacgtttgga tcttggtatg
    18361 tatttaatgg ttcgagaagg gaccgttgca aaagatctgg catcgctact accggcgatc
    18421 acacctgaaa attcaagaag atgcttcttt gtcaccgatg ataaactgat caatgattta
    18481 gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta ttgcacttgg aatcgatcca
    18541 ttacaagcca ttcagatggc aacattgaat gcagcagaat gttttgattt gaaaacacta
    18601 ggtgcgattg cccctgggta tcaagctgat ttcttcttaa cagacgatct taccaagctg
    18661 ccaattcatg aagttttcta taagggaaca ttagttgttc aatctgggca actccaagac
    18721 catttatttg ctgatcattc aaattcatat acgtgttgtc tgccaaaact aaacgctcaa
    18781 ccattaaatc cacattcgtt agagctgctt ctttcttctt cacaagccca tgtgatcgaa
    18841 attatcccga atagtctagt gactaaagat ttggtagaaa aagtggaccg acaaggcgaa
    18901 cgattcattc cttcaatcga aaaagaccaa ttaaagattg cggtgatcga gcgtcaccat
    18961 caaacgggaa acattggatt aggcatagtg aaaggatttc agctgaaaca aggagcgata
    19021 gcgacaacga ttgcccatga ctcgcataat ctagttgtcg ttgggacaaa cgacgaggat
    19081 atgctgtatg ctgctaatct tttgattcaa aaaggtggcg ggatgaccgt tgtcaacaat
    19141 cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa tcatgacaca ggaacctttt
    19201 tcgatcgtct atgaacagtt gaatgaatta ataactaaag cacatcaact aggagcagct
    19261 aaaacctttg atcctttttt gacactttct ttcttaacat tatctgtcat acccgaattg
    19321 aagatcaccg acaaaggatt attttcattt tctcgcttcc aattgattcc accttgtgga
    19381 aaaaccgatg aaataaatac attttaaatc aaataaatcc cagaaaatac attacatctg
    19441 taatgttcat tttctgggat ttatttatgg atattaatgt ttttatgaaa gcagaaagtt
    19501 agtttcacag acttttcttt ttggtcttat cttgaaattg agcttctact cgataaatac
    19561 gttgaccttt tgaagaaaat ttttcttcat actccgtcat aatattgcct tcaaattgac
    19621 tttgatgtaa atctaaccat acctgtttga ggatcatgcc atattgagaa aaacttgcca
    19681 gagaatactc aaacaatcct tgattatctg ttttgaaatg gatttctcca tttggacgaa
    19741 gaatctgttc atctacagca agaaaagtct tatatgtcaa tcgccgtttt tcatgacgtt
    19801 tttttggcca tggatcggaa aaattcagat agatttgatc tacttcgctg tcagcgaaat
    19861 actctgtcaa cgctgatcca tccacatgta gaagtttcag gttaggaagc ggttgttcga
    19921 tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat ttctactcca atataattga
    19981 tttcaggatg ggcttttgcc attccagtga tgaactggcc tttccccata ccaatctcaa
    20041 tgtggattgg atgatcgttt tcaaacaatt cattccattt tcccttccac aatgttggat
    20101 cactaattac aaaattcggg tgagccgcta acatttccgc tgctcccgga cgatttcgga
    20161 cacgcattat ttatctccat tcctttcctc taattaaaaa ggtctggcca aatctccgca
    20221 gagtttgggc caaacctcag ccactgaaaa ttcgttttaa ttgtaaaatt tcttcgttca
    20281 tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc ttggagcaaa ttcatggccg
    20341 cataccaatg aaatttttca agcatttcgt cagtcgggcg tatgccataa ctgatcagcc
    20401 actgattcca gctagacagt ggcacataat ttcctaaaat cgttgcaata tccaccgcag
    20461 ggtctgcaaa catgattgaa tcccaatcga ctagatataa ataattttta catactaacc
    20521 aattacgatg gtttacatct ccgtgtacta ctgataagtt ttccttatct attgacggga
    20581 tctgctcacg aagaaaacga tggacaattt gcagaaattg atttttctgg agttcttccg
    20641 gtaattcttt ttcatatttt tgcagcatct tctccggcgt catgacacga ccaccgatac
    20701 gggtcagcat ggttttaaga gaatttgaat gatgtaaatg gtacaaaaca tcaatcacgt
    20761 cgtttctttg accaatctca ttaggagtca gcaagcgacc ttccagccac tcttgtgctg
    20821 tcaaagtatc gccattcccc gttcgttttg tccaaacaag ttttggtgta attccttccc
    20881 ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat aaagactttg tcctcatcac
    20941 gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg acgtaaccgc cactcttggt
    21001 ctaactgaaa ctccatcatg cattctcccg tcctcttact ctttgttttg cagtacttat
    21061 tattctagtc gcctgaacac tacacgtcaa gcacatatac gttctttttc atttaattgc
    21121 cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa cagcattctt actgtcaacg
    21181 ctctattttt cattctcatt tttaccctac acagctttgt tttcagtctg acatcttctt
    21241 caaacgagtt ggtacatata gtttaacaaa gagcatagta acaatcaaat aagccgctag
    21301 cggaacccat cgctgctgcc catctaatac gactgcagca atcaaaccaa aaataaccga
    21361 cgcgataatt aatacccatg tgagtagata ctgaatcgcc tgcttcttct gattcttatg
    21421 gattggataa agctgaacga gaaccatata gcggaactgg ttcgctaaag gtaacagttg
    21481 gaaaccgatc aaatataaaa ataagaaacc gataatcaga ctaaaataaa agtcgactac
    21541 tccggcaatc aataacccac ctataattac taatcggaaa taaagaccac taaaatcaga
    21601 acctcttgcc aatcttcttg caaacaagta aagataagtg ttttttgatt ctcttttaat
    21661 atgagtcaat ataggatcca aatagcgtct tcttttgatc tttgccgtaa tttcaggaac
    21721 atctgtgaat aaattgataa attgatagat ctgatgcaac ctatgttctt ctacatgcac
    21781 cattttctcc caatccaatc ctttttcttg ttgcttccac aaaaaccagt aatatagact
    21841 tgcctgtact aatgcacctg ctattcctgc ccatattagc caataaaatg atagaaacaa
    21901 aaagatcaga gaagaaaaaa accagcctaa tttccaataa cgaataactt gttgatctag
    21961 tcgaaacaat tctgctctct tgatttgcag atgactgaat ttcaaactcc ataatatgat
    22021 cagatagacg aaaaacatcg aaaaggattg ctccgtacta accaccacta atggcatggc
    22081 caatccgcat gccaatatca aaaagatcaa cggaaaaatg caagaatagc gaaaagctcg
    22141 ttccaaatag gtttgcattt ctttttcttt aggaagtaaa aaaacaatat ctgctgcctc
    22201 tgcaaaagta gccatcttac ccacgaataa acagagcatc caaaatacag agattatgag
    22261 ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa ttagaataat ataatcccac
    22321 accgccaatc aagaaaagac aaactaaaac aaaatgatcg ttcaatacat aacgcatata
    22381 tttcatcatt ttcttttgat gtcttgctaa acgtttttga aaaaaactac tcatgtgcct
    22441 gctcctttgt taaagcaata tagatttcgt ccaaagaaga atccggcagc tgaaattctt
    22501 ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc gttatgtaag accacaaaac
    22561 gatcacaata tttttctgca gtagctaaaa tatgcgtaga catcaaaata ccggctcctt
    22621 cttttttcat ttctaccatc aattcaagca atgcattgat agctagtgga tccaagccta
    22681 aaaatggttc gtcgatgata tataaagaag gctcgatcaa aaaggcgcat aagatcatca
    22741 ctttctgttt cattcccttt gaaaaattag ctgggaacca gtcaagacga ttagataacc
    22801 gaaaagtctc caacaacttt tctgctcttt tcattgctat atccataggt atatcgtagg
    22861 ccatcgcagt gacttcgata tgttctctca atgtcaattc ttcatataaa gaaggagtct
    22921 ctggaatata cccaatttgt ttgcgatatt cttccgtatt tttctttaag gatagatgat
    22981 tgatttctat cgttcctttt gaagcttcca gcagaccgat aatgtgcttg atggtcgtac
    23041 ttttccctgc tccgttcagt ccaatcaacc ctactatctc tccaggctgt acatcgaaac
    23101 tgatatcttt taacacaggg agatgtccgt acccccctgt gacatgatcg ataattaaac
    23161 tcattctttt tcctccgcca tttattttta tttattatac cacgaagaaa atgacggtat
    23221 tttgtttttt tccttacaat agctttaact tatggtaaag ttaagctgaa gtatttattc
    23281 tttatagaaa ggatgtttta tatggaaaac tgtatttttt gcaaaatcat caatcaagaa
    23341 atcccaagct ataaaatata tgaagacgac aaggtctatg cttttttaga catctctcaa
    23401 gcaacgaaag gacacacatt agttgtgcct aaacgtcatg tagcagatat ttttgaatat
    23461 gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta agattgctca agcattggaa
    23521 aaagcttttc cagaaatgaa aggtcttaat atcttgaata acaaccgtga actagcctat
    23581 caatctgttt ttcattcaca tatccattta gtccctcgct acagcaaaga ggatgatttt
    23641 tctattcact ttgtcaatca tcaagatagt tatggatcgg aagaactaaa agctattcaa
    23701 gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac tttttcaaag gactgttatt
    23761 tggagctgct gctggcactg tcggaggtct gttattggcg cccaggagcg ggaatgaaac
    23821 aagaaagaaa ctgatcgaag aattggaaga agcaacaact ctgacaaatg atttaaataa
    23881 cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa gaaacagctg aatctgtgat
    23941 tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat tttaaattcc aagccgaacc
    24001 acgtatcaca caaatcaatg aacaagtaga aaaactaaaa gctgatctgc cagagtatcc
    24061 tgaacaaata taataatgat tattttctca acagatttaa ctatataagt gagaaaagtt
    24121 tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt ttgaaactac cacgacaacg
    24181 tttttaaagg aaatgggata tggtatagtt aatatataaa aataataact aattacagga
    24241 gtgcaaattt taatgaaaaa aaaatcaatt attttggcag ctactagtgc gttggctgtt
    24301 ttaactctag cagcttgttc aggcgatact aataaagata tcgcaacgat gaaaggcgga
    24361 acgatcactg tatctgattt ctatgatgaa gcaaaacttg aatcatcgaa ccaatcctta
    24421 gtacaacgga tgatcattta taaagtattc aataacaaat atggcgataa agtaaccgat
    24481 aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat taggtgatac ttttgaatca
    24541 caattagaag ctgctggata tacgaaagat acttataaag aatatattcg taacaattta
    24601 gcttttgaag ctggcttgaa agctcacgta gatattacag atgacgattt gaaaactgct
    24661 tggaaatctt tccatccaga agtagaagca caaatcatca aattatcaag tgaagatgaa
    24721 gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt tctctaaatt agccaaagac
    24781 aaatcaactg atacagagac aaaagaagat ggcggtaaag ttaaattcga ctcaacgaca
    24841 acaactattc ctgcagaagt aaaagaagca gctttcaaat tgaaagacgg tgagatctcc
    24901 gatgttatta ctacaacaaa tcctacttct tatgcgacag aatactacgt tgtcaaaatg
    24961 gtgaaaaacc aaaataaagg caatgacatg gataaataca aagatcaact aaaagatatc
    25021 gctactgaaa ctaaactaag tgacaatgca ttcacaacta aagtaatcgg tgaagaatta
    25081 aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa atgtgttgag tgcattcaca
    25141 acaacttcat caagtacgaa agactcaagt gaaactacag cttcaacaaa gagttctgac
    25201 acaaaatcaa ctgattcaac aaaagaatct tcaactgaag aaacaactga ttcatcaaaa
    25261 taaa
//
LOCUS       DANKMN010000045        24975 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000045 DANKMN010000000
VERSION     DANKMN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(43..2187)
                     /locus_tag="PW224_001722"
     CDS             complement(43..2187)
                     /locus_tag="PW224_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2908410.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2544..3917)
                     /locus_tag="PW224_001723"
     CDS             complement(2544..3917)
                     /locus_tag="PW224_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2908411.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="PW224_001724"
     CDS             complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="PW224_001724"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL2908412.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="PW224_001725"
     CDS             complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="PW224_001725"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL2908413.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5365..6378)
                     /locus_tag="PW224_001726"
     CDS             complement(5365..6378)
                     /locus_tag="PW224_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2908414.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6459..7058)
                     /locus_tag="PW224_001727"
     CDS             complement(6459..7058)
                     /locus_tag="PW224_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2908415.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7244..7945
                     /locus_tag="PW224_001728"
     CDS             7244..7945
                     /locus_tag="PW224_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2908416.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7955..10666
                     /locus_tag="PW224_001729"
     CDS             7955..10666
                     /locus_tag="PW224_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908417.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11117..12745
                     /gene="treC"
                     /locus_tag="PW224_001730"
     CDS             11117..12745
                     /gene="treC"
                     /locus_tag="PW224_001730"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL2908418.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12805..13986)
                     /locus_tag="PW224_001731"
     CDS             complement(12805..13986)
                     /locus_tag="PW224_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2908419.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14238..15761
                     /locus_tag="PW224_001732"
     CDS             14238..15761
                     /locus_tag="PW224_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2908420.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15830..16558)
                     /locus_tag="PW224_001733"
     CDS             complement(15830..16558)
                     /locus_tag="PW224_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2908421.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16571..17851)
                     /locus_tag="PW224_001734"
     CDS             complement(16571..17851)
                     /locus_tag="PW224_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2908422.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17992..18594)
                     /locus_tag="PW224_001735"
     CDS             complement(17992..18594)
                     /locus_tag="PW224_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2908423.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18839..19045
                     /locus_tag="PW224_001736"
     CDS             18839..19045
                     /locus_tag="PW224_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908424.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19357..20076
                     /locus_tag="PW224_001737"
     CDS             19357..20076
                     /locus_tag="PW224_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2908425.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20157..20840)
                     /locus_tag="PW224_001738"
     CDS             complement(20157..20840)
                     /locus_tag="PW224_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL2908426.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21058..23244
                     /locus_tag="PW224_001739"
     CDS             21058..23244
                     /locus_tag="PW224_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL2908427.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23590..24000
                     /gene="spx"
                     /locus_tag="PW224_001740"
     CDS             23590..24000
                     /gene="spx"
                     /locus_tag="PW224_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2908428.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            24014..24370
                     /locus_tag="PW224_001741"
     CDS             24014..24370
                     /locus_tag="PW224_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL2908429.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8367 a         4437 c         4872 g         7299 t
ORIGIN      
        1 aaaagggagt gtgacagtgt cacatcccct ttttaattta ttctatttct ttaaaggcag
       61 atcaagccag aatgatttgt ctaaacttaa caaggttagt acaggaacag ttgcagcttt
      121 aactgtacga gtagttgttt ctggtatctg tcttgccgta aaattgttcc acaaatccca
      181 gattgaagcg acacggaaga tggaagaagc gttgttgacg cgttttatcg ttgaaaattc
      241 taatcctgtg attggcacgc catctgttgt ccatttgatt ggcacatcga atcgcttaga
      301 catattgagg atttcagcgt ttgcttcttc agttgttgct gcttcttttt tcggttcatc
      361 atattttgtc aggtcgtacg cttcgatgat cgcgttcaat ttttgtgaat attggcgatc
      421 tgtagcataa cgtccttcca aatatttggt tgcttctttg tagtcttttg tttccgactt
      481 ccaagttggt ttgtagaaat caggattgcc agtcagtcca tttttgatca agtcggcata
      541 gtcttccaaa ctttccttgt aagaaggata tttacggaat tgtgcgcgga tttggtagct
      601 ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg ctgcttcctt tatatgatcc
      661 tttgatacca aaaaggttat gatttggttc gcttgataat aagctgtttc cagatccact
      721 ttcaaggata gcttgagcca tcatgactga tgcgtagatt ccttcttttt gcccgatttc
      781 ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt gtttggtttt gactgaaatg
      841 gaacgagcca tctaatccat ctaaaataga tgagccgcct gtcagcgaac ctgaagcagc
      901 aggtaaacct gctaagttta ctcggacagc aaaatcaggc gtgaaatctg aaatagaagt
      961 tactcgaact ttatctcctg gttgaggcgc atggataaat tgtccgttcc caatatagat
     1021 acctacatgg tgagtaacac cggaagaagg tccccagaag acgaggtcgc caggttgggc
     1081 ctgacttact ggaatctgcg tacctgcata ttgctgttcg cctgtccagc caccgatata
     1141 atgaccggtt gctttcatat atacgtaata agtcaaacca gaacagtcaa aagtatttgg
     1201 accttttgct ccccatacat atggttttcc aagatgcttg aatgcttctc gaacgattgc
     1261 ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca attggtgatt ttacaacaaa
     1321 atcagaagaa gcagaagatg gattctctat accgctttga tttgctgtat ttggacgaga
     1381 tgaagcattg ttcgttccgc cattcgatga accattgttt ggatggtttg gttctctctg
     1441 agtatttcca ttgttattcg atccagatgg gtttgtttga ttcgaagaat tcccgctgtt
     1501 cgaattattg ttattattgt ttgaattatt tgaaccatta ttattgttgt tattacccac
     1561 atctggtttg tttggcgttt ccggatctgg tgtactaggg acttcttcac taggatcagt
     1621 tggtttctct gttgaagtat ccggtccagc tttaaattct gcagtaatag ataaattgtt
     1681 cgtacctact gtaaaggtct ttgcatcata atttacttct ttggcaccat taacagcgac
     1741 tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa gttaccttcg tttccgctaa
     1801 taaaccttct accgttccac tttccgtaat atccaaaggt tgctgttcag gggaatccgc
     1861 tgtcgaagca gtcagttgga tcttaccaat caaagaagga gcaattgtaa gggaatatgt
     1921 ctcggctggc ttttcatttt ctgatggatc agccggatca atttcagttg gctcggtcga
     1981 tcctgttgtt gagttagttg ttccagttgg gtcttctgta aaagaatccg ttgtttcact
     2041 agtagctgag ctggatgtag tcgtatctgt tgtgactgtt tgatcagcta tttgattatc
     2101 tacggcaaat acggtattgg tcgacaaaac agacggagca atcagccaag tagcagctag
     2161 caaacgtatc gtttttttat ttttcataat aaactagtcc ttttcctttc attgattttc
     2221 tgtcttattc agcattcatt agacattttt tggaacagat aatcgtgtcg aaaagctttt
     2281 tcttttcata aatctgtctg tgtattctaa ataaaacaat ttgtaaataa gcaacgagca
     2341 ttcattcgtt tttctttgac gttcatttta ttgcatagac cacctttgtt tggggataaa
     2401 caagtgggga taaaaatggt tatgcatatg taaattatac caaagtttgg aaagtatcac
     2461 aaaaatttat aaaaataatg atcctaaaaa gaaaaagact gaaatatgtg ataaacatct
     2521 catacttcag tctttttcat taactattca taagacatta ggtcttcatc aaacccgcta
     2581 acgagagtag tgatcattgc tacgactaga ctaacagcca gtcctgctaa gtaccagaaa
     2641 aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac caaatacaaa agcgatcgct
     2701 ttcacttggg caaatcccat tacagctcca ccagcagctg caccaatgca agcagcaaca
     2761 aatggttttt tcaatgggat cgcaattccg taaacagttg gttcggtcac tcccgcaagt
     2821 aaaatcgaag caaaagcaga accggagatc gaacgggttt ttgaacgttt acttcgcaga
     2881 aatacaccta aagctgcacc agccattcca aaattggctg ctcctgtcaa cggaacgatt
     2941 cggtcgaacc cataagcaga gatattactg atcatgacag ggtttacagc ccattggata
     3001 ccaaatatta ccaaaatatt ccaaccacca gcgatgattg cgccactgat cggtccgtta
     3061 atatccatca tccaagtaat tgcggcagct aaaccttcgc caaggtaaac acccgcaggc
     3121 ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg aaagtaaagg tacaaatact
     3181 aattgcatat tttcgcgaat atattttttg aggaattttt caacaaaaga ataaaagaaa
     3241 atacctaaaa tcgccggcat gacagtagac gtatactgca tcagtactac aggaatcccc
     3301 agaaaatcag tagctgcttt gccactgtct gaaagtaaag aggtcagatt cggttcaatc
     3361 agtgctgcag caatgacagc cgcaatatat ccattcactt taaacgtttt ggcagcagaa
     3421 aaagcaagaa agattggcag aaaataaaag acggcattcg acgcagcaga caaaatatga
     3481 taagttccgc ttgtttcact aagccagcca atcgtagtcg ctaaaacaac cagacctttt
     3541 aatataccag aaccggcaaa cagcggaatg atcggcgtaa agatttccga tatcaaacca
     3601 aatagttttt cgatcggatt tagcttttct ttcggagctg aatgatcgat ttgacgcgtc
     3661 aattccttgt aagcaggagc caatccctga tccatgatta tttctgcata tctatcgagt
     3721 aaaatgaaat acttaatgga agggatttgc tgttttaatt tttctgcatt ggctactggc
     3781 tgttttttca atttgatacg aagccgagtc gcacatggaa ggacagaatc aatattgtct
     3841 tttcctccca actgttcaat cagcatatga atctgctcgg acagtaccat ctgttcagga
     3901 ttcattgtcg tttccattag aagaccactc cactcactaa tataatattg gcgtaaggtg
     3961 tcatttcgcc tgtacgaata aatgcatgac agttattcaa ctcttttttc atttcttcat
     4021 gtgggataaa gctaatcggt gtatctggta acaataattt gattttttcc aatatttccg
     4081 ggttcattgt ctttatttct tccgccaatt caatcgattg tacctcaagt tcttctaaaa
     4141 cgttttctaa aacagataaa aaacttggaa gaccgtttga gacagccaaa tctatttttt
     4201 cagtaccaaa ggggacaggc atgcctgcat ccccgatact taatttatca aagtgcccca
     4261 tttgtgcgat tactcgtgaa atatctgaat tgattacttt tccttttttc atgtttgttt
     4321 cttcctctca ttgttaattt tagactgctg attgttctaa agcttcgcgg taagggatag
     4381 aaggttgcgc accataagac tgcacagcaa cagaagaggc tagatttcca taaaggatcg
     4441 cttctttcaa gttactaagg tctggctgaa gtgtgctgct taatgcgcca ataaatgtat
     4501 cacctgcggc tgttgtgtca acggcatcca ctttaaaagc agggacgata ccgtgttctt
     4561 tttctgtatg atagaaagcg ccggcactgc ctagtgtaat gattactgtt ccaataccta
     4621 attcatggag tttttcggct gctgcgacca aactgttatg atctgtcact ctgattcctg
     4681 taatgatttc tgtttctgtt tcatttggta taatgatatc tgtcttttca agtagttcag
     4741 taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt tgtttttcct gcgtcttttg
     4801 caatagaaaa agctaggatc gtcgcatcta agctactttc aaactgagca ataataaagt
     4861 cactgtttgt aatcagttgc ttgtttttta ggacttcttg ttcatggaaa gcattatttg
     4921 cccctgcatg gatcaagata ctattttctc ctgctgcgtc taccataatc attgcttgtc
     4981 cggttgtggc gccttggatt tcagctatcc cagatgtatc gatgttttct tttgtcagta
     5041 gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc tccaataaaa gatgtccgtg
     5101 cgcctgaacg tttggctgcc actgcttggt tggcaccttt acctccgcca gcgtgaaaaa
     5161 tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt ggttaaacga agggtagtgt
     5221 ccatattaat acttccaatt actgtaaccg tgttcatttt acaaacctcc aactatctca
     5281 tttattttga tgggaaaatt attttttaaa acgttttagt aaaacgtttt aatagttcga
     5341 taaaaatata gcacaccttg aaagctattt caagggtgca gttgaaaatc ttttttctaa
     5401 tcgtacaggt aatctttttt cttcccattc tttttctggg aattggatcc gatctaacaa
     5461 taacttggca gatgtttgtc ccaattcgaa gattggttgt gcgatcgttg tcagtttcgg
     5521 cttgatatac tcgcacatat ctatattatc atagccaata atgctgtagt cttcagggat
     5581 gctttttcca gcttcctcta atccgcgata taatccaaaa gctaattcat catttaaagc
     5641 aaacacacca gttgcagcag aatcaagaag ttctgctgtg atctggtagc caccttgttt
     5701 tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg atttggtaaa catcaagagc
     5761 agatttaaac ccctcaatcc gagcgtggac attttccggt ggattttcag gatagacaag
     5821 ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc gctaggtaac caccgcgaaa
     5881 atcgtctgtt cgtacggcat cactaaatcc ttcagatttt ttctgatcca aaacaatgaa
     5941 gggccggcct tgttttttca aattttcgtt gattgcatct gtagagacag cagaagtcgc
     6001 gataatgaat ccatccacac ctcgacgtgt gagctcggct agatattcga tttctttttg
     6061 atgatcagaa tcagcattac ataatatcgt gacgaaattc tgcttataca agatatcctc
     6121 aattcctcgc atcagtgtac tgaagaatgg attcgtgata tcaggaacga gtacaccaat
     6181 tgttttcgtt tccttggtaa tcatttgtct agcaaaatag tcaggctgat agcctaattc
     6241 ttctttggca gctaaaactt tctgaacagt tttcggactg aatttctccg tatttccatt
     6301 taatatcaag gagacagttg tgattgaaac gccggaaagt ttagcgactt ctttgattgt
     6361 gatctttttt ttcaccatgt tcatactcct atctaaatcc tgtggttatt ttcccatgaa
     6421 atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt atttgctttg ttggatagct
     6481 tcgattattt gtttcgttga gcgtactcgt gcagttttcg gaaatatctt ttttatgaac
     6541 aattgatggg attctttgtc acgatcactg caagcatctt cagcaagcac gatatggtac
     6601 ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt ttgttgtcgc tacaccgctt
     6661 aaaatgatag tttcgattcc acgccggcga agttgaagat caagagaagt accaaaaaaa
     6721 gcacttggat tgtattttgt gatagtcaac atattatctg tatctttcaa agaatccttc
     6781 aaaagtaaat cagtgaattc tggaggtaca gaagcagtca ttccacccat atcagtttgt
     6841 tgactcaaat aagggaaagt ggagatgtct acgttcacta aaactttcaa ggcagcggta
     6901 cgtttcaatg ttcttactaa caaatcattg tttgtcagaa tagatgctgc agtgtgtgga
     6961 gagagtgtac cgttatttac gatctctttt tgcatgtcga tcgcgacaaa agcagttttt
     7021 gataaatcaa aaatcatatt ttcctttaaa tcattcatga tgttccttct ttcaaataac
     7081 aatgtttttt atgtaagtaa atcataaatc gtttatttgt caactaaatt aaaggattcc
     7141 tttctttagt ctagagaaat tcagtggtaa aataaataag tgtgagaaaa cgcattcgat
     7201 gcatagatat agcgagaaat atcgtgaaaa tggaggaatc agagtgaagt atgtcgaagt
     7261 aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc attacagcaa atgacgatat
     7321 ttctttttca attgaaaaag gcgaattagt gattatactt ggtccaagtg gagcaggaaa
     7381 gtcaacgatt cttaatattt taggtgggat ggatacacct gatgaaggtc agatcattat
     7441 tgatgacaca gatattgcgc aattttcaga taaacagctt actgcttacc gacgaacaga
     7501 tgtagggttc gttttccaat tctacaatct cgttcctaat ctaacagcaa aagaaaatgt
     7561 ggaattggca acggaagttt ctcctgatgc gcttgatcct gtagaggtat tgaggcaagt
     7621 gggcctagct catcgattga ataactttcc gtcccaatta tcaggaggcg aacagcagag
     7681 ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt cttttatgtg atgaaccgac
     7741 aggcgcgctg gattttgaaa cgggcaagca agtattgaaa ttattgcaaa acgctagcag
     7801 acaacacggc aataccgttt tgatcattac acataattct gctttagcgc ccatcgctga
     7861 tagagtcatt cacattaatg atgctaaagt aaggtctgtt gaattaaatg atcatccttc
     7921 atccattgat gagattgtat ggtaggggga ttttatgaaa acaagaacat atcttaaagc
     7981 aggttttcgc gaaattcggc aatctaaagg acgattcatt gcaatcatcc ttattgtcat
     8041 gctaggaaca cttttatttg taggtgtcaa aactgctgga cctgtcatgc aaaaaacgat
     8101 ggatcactat gttggtacag caggactttc tgatttgcaa atcgtttcta ctggtggctt
     8161 gacacaaaaa gatattgctc aagcagaaaa gatcccagat gcaaaagtag aaacaggaaa
     8221 acagatctat tattccaata ctggcaaaaa cgaagtgatt caaatatttt cttataacaa
     8281 aaataacaag cagaacaagc tacaattaac tgatggaaaa ctcccggaaa aaccaaacca
     8341 gcttgtattg gatgaaaaag cggaagatga agggtataaa atcggtgata tttatcagat
     8401 tgattcagat gaattgaaag aaaaagagta taccatcacg ggctttgtcc gttctccatt
     8461 gttcatcaac aatttggaga gaggatacgc gaacgtcgga aatggtagcg tcgattattt
     8521 tgtctattta cctgaatccg tttttaaaac agatatttat tctgtcttat atcttgattt
     8581 tacaaacgta aagaatttga atacgtacag caaagcttac aaagataaaa tggagcaaaa
     8641 tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa gaacgcttag aagagctgaa
     8701 aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa atagcggatg gcaaagcaca
     8761 aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa cagttagcgc aacagtcagc
     8821 aactatccag cagcttcctg aagcgcaacg aacaatcgct gttcaaacat tatctcaaca
     8881 agaagctcaa cttgctgaac aagaaaggca attagttgag aaagaacgtg aactggcctc
     8941 tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa aatttaacta aaccatcata
     9001 cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa tttggtgatc tgtctgatcg
     9061 tatcgctgcg attgccaatg tttttcctgt tttctttttc tttattgccg cattgatcac
     9121 ctttacgaca atgacacgta tggtagaaga aaaccgcagg gaaatcggga cattaaaggc
     9181 gttaggttat acgaaagttg aaatcgcaaa aaaatatgca atctatgcaa gtctagcctc
     9241 agggataggt attgttttag gaacgatatt aggaacgaat cttttgccaa ggatcatttt
     9301 tgagctgtct aacgagcggt atgatattgg gagtgcgctt attttttatg actggccgcc
     9361 gattttacaa gcagctgccg cattcttcat tgccgcattt ggcgctgcaa tgatcgtttt
     9421 attcaaagat ttaagagaac ggcctgctgc attgcttcaa ccgaaagcac cgaagccagg
     9481 gaaacgcatt tttcttgaat acatcactcc gctgtggtca cgccttagct ttaatcaaaa
     9541 aatcagttac cgaaatctct tccgatataa gtcaagaatg cttatggcga ttatcggaat
     9601 tgctggatgt gccgggctga tggttgcagg cgtgggattg aaagattctc taagttctgt
     9661 ttcagccaaa caatttggac cgatcattga ctatcaagca atcgttacat tggattcatc
     9721 cacggaagat agtcgtcaaa aagcagcgga tatgctgcag aagcaagaaa aagtaaatga
     9781 gatcctagct attcatactc aaaccgtaga attgagaaaa aaaggacatg cttcgcaaag
     9841 tctgacagtg atcgtaccaa gtaaaacagc tgatttggat cgttttatcc atttaactga
     9901 tcttgaagag aacccacgct ccttgcctaa aacaggaatc gctattacgc aaaaagtagc
     9961 agaactattt gacttatcta caggagatca tatttcgctt tatgatgaag atcaaaaaga
    10021 atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta ggtagcttta tttatatgtc
    10081 ccctgaatat tatgcttcag ttactgatca atcgatgatg gataatgcat ttttgctgcg
    10141 tacagataag atgacaacaa aagaagaaga aatgctttcc gagaaactgc ttgccacaga
    10201 cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa acgcaagaag aatcaatggc
    10261 gaacttagac tcggttgtct taatctttgt cgtattatca ggactgctgg cttttatcgt
    10321 cttgtataat ctaactaata tcaatatttc cgagcgtgta cgcgaattat ctacaataaa
    10381 agtccttgga ttttttgata aagaagtcac gatgtacatt gttcgagaaa atatcatctt
    10441 tactctttta ggaatcatcg gaggatttgg tgtaggatat atattgactg acttcatctt
    10501 gcaacaagcg tctatggaga atgtcatctt tccattagtg atcacatggt gggcttatgt
    10561 cttatcagca ggattaacta tcgtatttac ggttattgtg atgattgtta cccatttcaa
    10621 attaaagcat atcgatatga ttgatgcgtt gaaatcgaat gaataaaaac agaggttgtg
    10681 aaatcagcgg tttgacctga gtattagaga aacaaacggc aaaatagttt ttcatatttt
    10741 gccgtttttt gagctaatac cgaaggacag ctgattgaac accatgaatc agaggttgta
    10801 aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata aaattagctc ctcatgtttt
    10861 acatttgaga agactgattg ccgtagataa aagcttttga acaccattta tagaagaaac
    10921 aagaatattt tgctatttta aacaagagga tagacaaata catgtctgat ctcttgtttt
    10981 ttattgggca acagacctct tagaatgtat aaagataaat tttacaaacg ctttcggttc
    11041 agttataata ataaatgata acttgtatat ataagttgtg gtaggtaata aatcagttga
    11101 atggaggtaa atatccatga catttaaaga caaagtcatt taccaaatct atcccaaatc
    11161 ttacaaagac agtaatggtg atggaattgg agatttaaaa ggaatcaaag aaaaactccc
    11221 gtatctgaat gatctaggca tcgacatgat ttggctaaac cctatttatc caagccctca
    11281 aaaagacaac ggttatgata tctctgatta cacagccatc gatcctatct ttggtacgat
    11341 ggaggaattc gaacagctaa tagccgaagc aaagaaatac cggatagaaa tcatgctcga
    11401 tatggtgctg aatcacgttt cgacagaaca tgaatggttc aaaaaagcac tagcagggga
    11461 ccagcattat caggacttct tcattttaag agaagagcca acggattggg tatcaaaatt
    11521 tggtggaagt gcttggtcgc catttggaga tacggggaaa tattatttgc acttatatga
    11581 tcggacacaa gctgacttga actggcggaa ccaagcagta agagaggaac tattcaaagt
    11641 tgttcgtttt tggatggaaa aaggggtaaa aggattccgt ttcgatgtta tcaatgttat
    11701 cggtaaagac gaacaattga aaaataataa agaaaatgat ggcaaacctg aatatacgga
    11761 caaaccaatc acccatacgt atctaaaaga attgaaccaa gcaacgtttg ggacggattc
    11821 cactatcatc acagtcggtg aaatgagctc tacgacgatc gaaaattgta tcttgtatac
    11881 agaacctagt agagaagaat tatcaatggt ctttaatttc catcatttga aagtcgatta
    11941 tgaaaatggg aataaatggt ctactcctcc atttgatttt gcagaattaa agcgactatt
    12001 tcatacatgg ggagaaaaga tgagtgaggg taacggctgg aatgctctat tttttaataa
    12061 tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc gatcagtatc gaaaacaagg
    12121 agcagagatg ctagcaacca tgatccattt gaaccgtggg acgccctata tttatatggg
    12181 agaagaaatc ggcatgatcg acccagacta ttcatcgatt tccgactata ttgacgtaga
    12241 aagtttgaat gcttataagt tactccttga acaaggattc acagaaaaag aagcgtttcg
    12301 tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc atgcaatggt cagacacaga
    12361 acaagcagga tttactacag ggaaaccttg gttaaaagtt ggaggcaagc tagaagagat
    12421 taatgtagaa aaagaactat cttctgaaga ttctattttg tcctattaca agaaattgat
    12481 tcggctgaga aaaacatatc caatcgttgc acaaggagat tatcatgctt atgaagcaaa
    12541 tcatccggaa gtttatggct ttcttcgcca atttgaagga cagcagctgc ttgtattaac
    12601 aaatttctat gataaagata caaggatcgc catcccagaa ggattcacag aagccaatat
    12661 tcttatcgat aattattccg atactgttct tcatcaaagt ctggagctaa aaccatacca
    12721 ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa cgtcgttcct acgagctatt
    12781 ctcgtaagat cgacgttttg tgttttattt aaaataatct tcttcagaaa atgaagagtt
    12841 ttcatactct cctttatcgc cataaaattt attgtagcgt tgtttgaatt tacggaaaat
    12901 aaaagtaacg agtaatttga ctaacgtata gataggaata ccaaaaataa cgcccacaat
    12961 tcccattaga tcacccatga caagtaaaac gattaagatc gtaatcggat ggatctgtaa
    13021 acgatcaccc attactcgag gaatcaccaa gtcaccgtgg agaagttgta cggcaaacca
    13081 gacaatgaca aatttgacag ccataaaagt agagtcttgg aatgcaatga tcaaacctgg
    13141 aaagaaggcg ataaacggcc caatatatgg aatgatacat aacaacccag cagaaatagc
    13201 taaaacacta gcaaattcta atccgatcag taagaaacaa acccagtaga ctgctccaag
    13261 gataaatgaa gcaatgattt gcccctttag aaatgaaccc aattgttgat tagcaatctt
    13321 tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca atcgctaaaa ctgactgata
    13381 aaatttttga ggatctctca aaaggaaaaa agcgacaatt ggaccagtaa ataaagtaat
    13441 aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt tgtgcgccac tttcccaata
    13501 atttccaata aatccagcta tatcagacgt cacgtcgtca atagaagcga aaaattgatt
    13561 aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg agactctctt gaaaatcttg
    13621 aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt tcttcaagaa aaggaataag
    13681 ccatatgctt ccaagcccca ataaaaataa aataatcaca taaacgataa tgactgacaa
    13741 gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt tccaaaagat aatacaagat
    13801 cagtgcaaac aataatgggg gcaagatagt agcgataatg attccgacag gcttgaatat
    13861 aaacgagatc tgatggaaaa aataaatgat cagcgcaagt aagataacta ttcctaaaac
    13921 atagtaagag gtttttcctc ctaaaaagcg gatcaaacga ttcgttgtat ctgtttcttt
    13981 tttcactctt tttcctcctt tatctataac ttcattatag acaaaagcgg gtataaaagg
    14041 aaaatgatag aggttgattg caaaaatatc gtcaatcatt catataaact ttctcatcaa
    14101 ttaaaataag aaaacgcttg acagttcttt taaccatatg ataaattaaa tcctgttgct
    14161 aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac attcaaatga aagaagatga
    14221 gaagaggagt gtcataaatg aaacgattga taggtatatt ggccatgctg gttttagcag
    14281 gtatgttaac tgcttgcggg gcaagtggga aagccgagga cagtaaagaa aaattgagcg
    14341 tgatgacgac cttttatccc atgtatgatt ttacaaaagc aatcgtcggg gatgaaggag
    14401 aagtagagtt attgattcca gcagggactg attcccacga ttatgaacct tctgctaaag
    14461 acatggcaaa gatccaagat acagatatct ttgtttataa tgatgaaaac atggaaacat
    14521 gggtccccgc aatccaaaaa acattacaag aaggcaatgt ccatacgata aaagcgacag
    14581 aaggtatgct tttacttccg ggaagtgaag aaggacatga tcatgaccat gaacacgggg
    14641 aagaaggcca tacacatgag ctagacccac atgtatggct agctccaagt ttagcaatca
    14701 agcaggtagc aaacatacgt gatcagctga ttgaagctta cccagaaaaa caggaagtat
    14761 ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc actccatcag ctttatcaag
    14821 aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca acatgctgca ttcaactatc
    14881 tagcgttaga atatggattg aatcaagtat cgattgctgg tctttcttct agtgaagaac
    14941 cgtctgcggc gagaattgct gaactgaaac actttgttaa agaacatgga atcaactata
    15001 tttattttga agaaaacgcc aaagatagta ttgccagaac gttagcaaac gaagcaggtg
    15061 tatctttaga agtattgaat cctttggaag gattgacgaa tgaacaaata gaaaatggag
    15121 aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt gaaaaaaacg acagagacag
    15181 aaaacccgct agaagatact ttgaccccca aaaaagaaaa gagcgtctac aacggttatt
    15241 ttgaagacga ggatgtacaa gatcgctctc tttcagattg ggcaggaact tggcaaacag
    15301 tagatacgta tgtagaagat gggacttttg atcctgtttt tgaatataaa gaaaaattga
    15361 atcaggataa gacagcacaa gaatataaag aatattatac gaagggctat caaacagata
    15421 ttgcttcaat caagatcagt gagaaagaga tggcatttac gtttaagaat ggaacaacga
    15481 aacgttcaaa ttatcgttat gtagggaaag aaatcttgac ctacgaggca ggaaaccgtg
    15541 gcgtaaggta tctattcgaa gcagaagata aggaaagtgg cgcttttaga tatattcaat
    15601 tcagtgatca cacgattgca ccggctgact ccgaacattt tcatttatat gcaggaaatg
    15661 aaagtcaaga agtattgctg aaagaaatgg agaattggcc aacattttat ccagatacaa
    15721 tggatggacg tgaaattgca caggaaatgc ttttgcactg ataggcaaaa aagaaccgaa
    15781 gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt tatgaaatca
    15841 atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac gtgacttctc
    15901 tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat ttatgacaac
    15961 ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat tgtaattggg
    16021 catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa aaatattgga
    16081 tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag atatcttttt
    16141 ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg acattttctt
    16201 ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg ctttttaaga
    16261 ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa ggatctaatt
    16321 cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt tcgatatcta
    16381 aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt tcttccggat
    16441 cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta tttttgatca
    16501 ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg ggaatcatat
    16561 cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg ttttcaattg
    16621 atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata aacaagccaa
    16681 ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac tggtttaacg
    16741 gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc ttcttgagta
    16801 tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca ttgtccaaga
    16861 taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg attggctcag
    16921 gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt aatgacagta
    16981 aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc ggaaaaaggt
    17041 tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat ccttctaagt
    17101 cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca tgtttgaatt
    17161 ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc atcctgactt
    17221 gttgctctaa taactgatca gctaatttct tattagagat tttccaataa taaaccaaga
    17281 tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct aagacatcag
    17341 gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat aatatcaatg
    17401 tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct aacgattttt
    17461 ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga aaatactgca
    17521 atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgccg atgcttgtta
    17581 agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc aattgttgac
    17641 gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt agagcagtaa
    17701 aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga atgactcccg
    17761 caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt gtgtaaatca
    17821 aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga tttttaccaa
    17881 attatagcac aattggcgta ttattttttt aatttttcag ggattttgaa ttcagaactc
    17941 ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg tttaatgatt
    18001 gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag gaagcaacgt
    18061 agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag ctgagaaaaa
    18121 taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata atgggggacg
    18181 tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa gcagtaacaa
    18241 aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt gcagatgttt
    18301 catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa cgaattcaga
    18361 gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa aggtgattcc
    18421 tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta gttttccaag
    18481 attattcaat aaaacttcta agacaaattt tacttggaca tacaccatat cttcttccga
    18541 tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt tcatctcata
    18601 cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg tttcataaaa
    18661 agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt tccaagaacc
    18721 ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag tttgtgcttt
    18781 ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag ggattcttat
    18841 ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg atttatttga
    18901 atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat
    18961 gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt tagttgtacg
    19021 caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata aagaggttgt
    19081 gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct tttcatgttt
    19141 tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt tgaaaaaagg
    19201 gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac ttttggctac
    19261 ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc tataaatgaa
    19321 gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt gatcattgtt
    19381 gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca agatttagaa
    19441 acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt ttattctaat
    19501 ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt tttggcaatc
    19561 aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga agatagtatg
    19621 ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa gcagtatgaa
    19681 ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa gaaaccagat
    19741 ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa aaaaagaggc
    19801 cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa agaactcaat
    19861 cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt gatcgatggg
    19921 gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca agaaatcaag
    19981 atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta cactacaaat
    20041 aacccgaaag caaagggaat ctttacaact aaataatata aaaactaagc aaattgctca
    20101 ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca ttgagatcaa
    20161 atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt gagggccatg
    20221 atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg atgacagaaa
    20281 acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg gaaaaatcga
    20341 tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc tacccctttt
    20401 aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc aaaagctacc
    20461 cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg ctcatggacg
    20521 ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa caagcgccag
    20581 aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag aatccctaag
    20641 aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc gtcaaatgct
    20701 tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc atattggata
    20761 taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat tgtacgcccc
    20821 tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg tcttccttat
    20881 cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc atatcgcttc
    20941 ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac gaaactttaa
    21001 ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat caatcagttg
    21061 aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa caagtataaa
    21121 aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga agcggcttat
    21181 ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga aggaaacagc
    21241 tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga acaaaaagca
    21301 gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg gagcggcaaa
    21361 acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt agacgaagaa
    21421 cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa acgagtagat
    21481 aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat cttttttagg
    21541 atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat acaaggagac
    21601 ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat acgtaaatat
    21661 aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt agactattgg
    21721 cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa atatgattcc
    21781 attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct cacggaactc
    21841 caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca aaatctgaaa
    21901 agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga ttatctattc
    21961 attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg gatgacaaag
    22021 gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta tggattcaga
    22081 ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa aactcttttc
    22141 ttattaacta attatcgttc tcgggcagct attgtccaag gagcaaatag attgatcagc
    22201 tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga agcaggtgtt
    22261 atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat caaacaaatt
    22321 cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata ccgatcagct
    22381 tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt tgtactggaa
    22441 gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt ctatcaaaaa
    22501 attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta ccagcaattg
    22561 ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca atacttttct
    22621 cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc caatttaaaa
    22681 tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga ccagcatcaa
    22741 gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa ggaaatcgac
    22801 cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt cagaagactc
    22861 caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg cttgattgct
    22921 tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag taaaggtctt
    22981 agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta taacggagcg
    23041 acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtaac gacgatagaa
    23101 gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct gtatctatgt
    23161 gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa agaaaccggg
    23221 agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg tcagtttcaa
    23281 gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cagcttattt
    23341 tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgtga caagaatttt
    23401 gtcacacctc ccttatttat tttgaaaatg agaatactat tatcaaaata gagaatatat
    23461 agaaaaattc tcattattca aacgtaaata atcagttatt ttgatttttt ttatatttta
    23521 ttagctaata attggtattt taaaagaaat tactgtagaa ttggattata attaaaaagg
    23581 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
    23641 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
    23701 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
    23761 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
    23821 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
    23881 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
    23941 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
    24001 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
    24061 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
    24121 cgatttaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
    24181 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
    24241 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
    24301 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
    24361 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
    24421 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
    24481 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa
    24541 tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc
    24601 aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga aatgacatga
    24661 taagataact gagttgttaa aaaagaacca cttgtgaagc ttgattctat cgaaaaaaac
    24721 ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg ataaatgagt tgctgtaaga
    24781 tttcgaaaaa cgaaatcaaa aaaactttta aaaaagagtt gacaattact tgtctctctg
    24841 atatgatata aaagttgcta cggcagccaa aaacgaatcg gggaacactc ctgatgaagt
    24901 agacctttga aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc ttgattcaat
    24961 tcaagacaac aaaca
//
LOCUS       DANKMN010000046        24734 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000046 DANKMN010000000
VERSION     DANKMN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            50..1309
                     /locus_tag="PW224_001742"
     CDS             50..1309
                     /locus_tag="PW224_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL2908430.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(1398..2345)
                     /locus_tag="PW224_001743"
     CDS             complement(1398..2345)
                     /locus_tag="PW224_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2908431.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2739..4031
                     /locus_tag="PW224_001744"
     CDS             2739..4031
                     /locus_tag="PW224_001744"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL2908432.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4046..5815
                     /gene="aspS"
                     /locus_tag="PW224_001745"
     CDS             4046..5815
                     /gene="aspS"
                     /locus_tag="PW224_001745"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL2908433.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            5924..6799
                     /locus_tag="PW224_001746"
     CDS             5924..6799
                     /locus_tag="PW224_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2908434.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            7065..8948
                     /locus_tag="PW224_001747"
     CDS             7065..8948
                     /locus_tag="PW224_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2908435.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(9023..9307)
                     /locus_tag="PW224_001748"
     CDS             complement(9023..9307)
                     /locus_tag="PW224_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2908436.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(9297..10031)
                     /locus_tag="PW224_001749"
     CDS             complement(9297..10031)
                     /locus_tag="PW224_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL2908437.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            10160..10783
                     /locus_tag="PW224_001750"
     CDS             10160..10783
                     /locus_tag="PW224_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL2908438.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            10975..12096
                     /locus_tag="PW224_001751"
     CDS             10975..12096
                     /locus_tag="PW224_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL2908439.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            12103..15231
                     /locus_tag="PW224_001752"
     CDS             12103..15231
                     /locus_tag="PW224_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL2908440.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            15250..18444
                     /locus_tag="PW224_001753"
     CDS             15250..18444
                     /locus_tag="PW224_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL2908441.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(18590..19297)
                     /locus_tag="PW224_001754"
     CDS             complement(18590..19297)
                     /locus_tag="PW224_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2908442.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            20062..20460
                     /gene="spxA"
                     /locus_tag="PW224_001755"
     CDS             20062..20460
                     /gene="spxA"
                     /locus_tag="PW224_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2908443.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            20792..21445
                     /locus_tag="PW224_001756"
     CDS             20792..21445
                     /locus_tag="PW224_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL2908444.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            21671..22669
                     /locus_tag="PW224_001757"
     CDS             21671..22669
                     /locus_tag="PW224_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL2908445.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            22845..24653
                     /gene="pepF"
                     /locus_tag="PW224_001758"
     CDS             22845..24653
                     /gene="pepF"
                     /locus_tag="PW224_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL2908446.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT         8321 a         4237 c         5190 g         6986 t
ORIGIN      
        1 aagttaagct taaattatac aagttatttt ttataggagg ttgctgaaga tgaaagaagt
       61 accagtagta gatatcaaaa tcacagattt aaaaaaattg tatcaaaaag gatacaacat
      121 gttggaggag ttacgccatg aagcggacgc tcccgtcgtc aaagcaaaga ttttcaataa
      181 agaagctatc acgatctacg gatcatcagc agcaaaagta ttttatgatc caaggaattt
      241 caaacgaaaa ggtgctatgc caaaattagt attgaagaca ctttttggtc aaggtggagt
      301 acagactttg gatggagctg ctcatcacca tcggaaaaat atttttatgg atctaatgac
      361 accggaaaga atggaagatt atcatcgtat cttagataag aatctgacac aagcacttga
      421 agcacagcat ggtcaatttg aactatttga tttaagtaag atggttttct ttacatctat
      481 ttgcgagtgg gcaggtatca atttatcagc gatttctaaa gatgaggtcg aaaaattagc
      541 agaatatcaa atctctatga tcagcggaac gtttacttct ccgattgatc acataaaagg
      601 tgtcgaaaat cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag aagctcgaca
      661 aaatcctgtg gctggaaaag aaaatgtggc attatatgct tttgccaatg ccacagattt
      721 agatggtcag cttttacctc tggaagtggc agctgttgaa ttattgaaca tcatccgtcc
      781 aacggttgct ctaactgtgt gggcggcatt gatgggacat gcacttttca gtagaccaga
      841 tttgtatcag caattaaaaa atgacttttc gacgttacaa gatccgttta ttcaagaaat
      901 gagacgctat tatcctttct tcccaatgct acctgcgatt tcgctgaaag aagtagaagt
      961 agatggttat cgtataccag aaggcagttg ggttatttta gatttatatg gaacagacca
     1021 tgatgaacga acagttgaag cgccagattc ctttatgatc aaacggtatg tagggaaagc
     1081 aaaagatatt tcctataaag aagaatatga gatgattgca caaggcgggg gaaattttcg
     1141 acaaatgcat cgctgtgcag gagaatggat cactcttcat agtctacgtg tgttttccga
     1201 tcagctagta aataagtttg agttctctgt acctgaacaa gattggacga ttccattcaa
     1261 tcagtttccg acttatccga atagccgagc gctactttac aagaattaga ttagcataaa
     1321 cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc ttttttcata ttactataaa
     1381 ttgataaggt caattgatca aaatccacga aatgcttcat caatggtttg ataatcttca
     1441 ttggataaat gcaaatctaa agcttggaca ttgtcagata cttgctctgg acgcttagct
     1501 ccaggtataa ctacagacac tcgtggattc ttcatgtacc aagctaaaac aagttgcgca
     1561 ggagtggctt ggtatctttt agcaatgggg cgaagtacat cgacagcggt taaaatttct
     1621 ctgaaacgtt ctccttggaa atctggattt ctttttcgag gatctccttc accaaattgt
     1681 ttttcctcac caagttcata ttttcctgtc agcaaaccgg aagcaagagg aaaataggga
     1741 acaaaactga ttttgttttt ttcaagataa gggaataatt ctttttcagc ctgccgatgg
     1801 atcaaactgt atttatcttc tactacatcg acatatccat ctgcattcgc ttctttcaat
     1861 tgttctaacg tgaagtttga cacaccgact gctcggattt ttcctgcttc ttttaattcg
     1921 tgcaatgtag caactgattc attttttggt gtgctttcat ccggaaagtg gatataaaaa
     1981 atgtcgatgt aatcagtttg cagacgtttt agtgcatctt ccaccgattg tttgagaaac
     2041 tcaggagaat tatcaaatgt tcgcccttta tttggtacat gagcagcttt tgtagcgatg
     2101 acgacacgtg agcgatcata ttctttttcc ttcagtacct cgccaatcag ttcttcggaa
     2161 cggccgtttc cgtaagcata agcggtatcg atcaaattga tcccgctatt caaagctgta
     2221 cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa aaagattatg tcctccaacg
     2281 gcatttgctc ctaatccaag ctgttctgcg tagacttgtg aatgtccgat tcttatttct
     2341 gccatagctg attcctcctt tttgtctagt ctaaaacaaa ataccaaaaa gctgaactaa
     2401 aacgtttttg tgaggggaaa tagtcagata tctcttgact ttgagactgg tttttagtag
     2461 gttataagta atatcgaatg tcaatgaaag agcgagtaag tttttgaaac tttacaagag
     2521 aggatcatcg aggctgaaaa tgatctaaag tttcattagc ctaaagacac tctggagatg
     2581 gacgagtgaa aagccgtccc gcgtacgagc gttattcgta agaggaaaga gaaatctttc
     2641 aaaattaggt ggtaccgtgt atgaatttca ttcacccttg ttcatgatga acaagggtga
     2701 tttttttatt tacatagcta agaaagggag agaaaactat gagtttccaa agaccaaaag
     2761 gaacaaatga tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac
     2821 gtttattatt cagagattat cagtataatg aaatgcgtac accgattttt gaacactatg
     2881 aagtgatatc ccgaagtgta ggggatacaa cggatatcgt ttcaaaagag atgtatgatt
     2941 tttatgacaa aggggaacgc catgtgacgc ttcgaccaga aggtacagca ccaatcgttc
     3001 gttcttttgt tgagcataaa ttgttcggcc cagagtatgc gaaaccgttc aaaacttttt
     3061 atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg acggttacgt caattccatc
     3121 aaatcggagt agaagcattt ggatcagaaa accctgcaac tgatgtggag agcatggtga
     3181 tggcacttga tttcttcaaa cagttgggga tcacgcaaat ccggctagtc atcaattcat
     3241 taggtgataa agaaacgcgt caagcctatc gtcaagcatt gatcgactat cttttacctt
     3301 ttgaatcaga gctgagtgaa gactctaaac gccgccttca tgaaaatcca ttacgcgtat
     3361 tggacagtaa agacaaacga gatcaaaaat ttgtggcaga agctccatct atcttagatt
     3421 atctaagcga gcctgctcaa aaacattttg ataccgttgt ttccatgctg gatgcattgg
     3481 ctgttcctta tgaaatcgac agtaatatgg ttcgcggact agattattac acacatacga
     3541 tttttgaaat catgagtgac gcaccaaaaa tgggggcaca agcaacgatt tgcgcaggcg
     3601 gacgttatga caatctagtg gaagaactag gtggtccttc aacacctggc ttcggatttg
     3661 caatgggtat cgagcgatta ctcttgacta tggaggcaga ggaagtagtt atccctgcat
     3721 ttaatgagtt ggatgcttac gtggtcgcac tcggagacga aacaaatatc gaagcattga
     3781 aagtggtcca agcgattcga aactttggtt tttcagctga ccgcgacttc atgaaccgta
     3841 aagcaaaagc gcaatttaag acagcagata aagcgaatgc caaattagta ttggtgatcg
     3901 gtgaagatga gcttgctagt caaacgatta atgtcaaagc aatggcaagc cgaaaagaaa
     3961 aaagttttcc attagaagag atatatgaac attttgatga agtctatgat gaaatgactt
     4021 tattcgagta ggaggaaaaa aataaatggc acaaagaacg atctattgcg gattagtttc
     4081 cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg gtacaaaaaa gacgagacct
     4141 tgggggagta atctttatcg acctacgtga tcgtgaagga atcgttcaaa tcgtctttaa
     4201 tcctgaaatc aacaaagaag catgggaaat cgcggataaa tgtcgcagtg aatacgtgat
     4261 cgaagtgact ggggaagtta gaagacgcgg agaaaatgca gtaaatccta aaatggccac
     4321 tggtgaattc gaagtgattg caagtgaaat cgtgattttg aatcaagcaa aaaccccgcc
     4381 atttcaaatt gaagatgatc aagcaatcag tgacgaaata cgtttgaaat atcgttattt
     4441 ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta agaaatgcga caacaaaagc
     4501 gattcgtcat tatttagatg ataatgactt tctcgatatc gaaacacctt acctagggaa
     4561 atctacacca gaaggcgcaa gagactacct agttccttca cgtgtccatg ctggacattt
     4621 ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg ctgatgaatg ctggattcga
     4681 ccgttattat caaatcgttc gttgtttccg cgatgaagat ttacgtggag accgtcagcc
     4741 tgaatttacg caagtagata ttgaaacaac gttcttaaca gcagaagaaa tccaaactta
     4801 cacagaggga ttgattgcga aggtaatgaa agaagttcgc ggtatcgaag tcacattgcc
     4861 atttcctcgt atgacctatg atgaagcaat ggctcgctac ggcagcgaca aaccggatac
     4921 acgttttgat atggaattga tcgatctaag cgatacagtc aaagaagtag aattcaaagt
     4981 cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca ttgaatgcta aaggcgctgc
     5041 agatcgctac tcacgaaaag acatggatca gttaggtcaa tatgtaggac aattcggagc
     5101 taaggggctt gcatggctga aagtagaaga agacggattg aaaggtccaa ttgctaaatt
     5161 catgggagaa gcaacagaag caatcattaa agcaacagat gcaaaaccag gtgacttgct
     5221 gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca ttaggtgcta tccggacacg
     5281 tttaggaaaa gaattaggac taattgatga atcgaaattc aatttcttat gggtaactga
     5341 ttggccacaa tttgaatttt cagaagaaga aggtcgatat gtctctgctc accatccatt
     5401 cacgatgcca aaagaagaag atatcccgtt attggctgaa gaccctgcaa aagtctatgc
     5461 agaagcttat gatatcgtct tgaacggtta tgaactaggc ggaggttcta tccgtattca
     5521 tactcgtgag cttcaagaaa aagtactaga aactctaggc tttacaaaag aacaaatgga
     5581 agaacaattt ggtttcttgt taacagcttt agattacgga ttccctccac atggcggtat
     5641 tgcattagga ttggaccgtt ttgcaatgtt attagccgga gaagataata ttcgtgaagt
     5701 cattgccttt cctaaaaacg gaaaagcagc agatgtgatg agtgatgcac caagtatggt
     5761 ttcgccttta caattgttcg aattgaatat tgatgtaacg aatatagaag aatagccgat
     5821 aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac aaccttgatt taattgaaaa
     5881 acactttata atgaagccat aacatataaa gcggggcttt tccatgaaaa aaacattcga
     5941 aaattgggct tatcatgatt acacgaccaa gctttcggta tctatcgttt atgcgttgat
     6001 cgcatcagtc gcattgaatt tcttttatca gccaggacat atttattcaa gtggtattac
     6061 tggtctagct cagattttga cgactttgtc aaaatcactg ataggattcg aagtaccggt
     6121 atcaacaact ttgtatttac tgaatatacc tttgtttgta ttggcgtggt taaaaatcgg
     6181 gaagaagttt acaattttca cttttatcac agtcactttg acctcccttt gtatccatat
     6241 tttaccggaa acagtcctat ctaccgatcc aatcatctgt gcgttgtttg ggggggctgt
     6301 gatgggacta ggtattgggc tagcgttgag aaacggccta tcatcgggcg gtctagatat
     6361 cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt ggttctattt cgatctactt
     6421 caatgcattg atcgtttttg ttgccggaat tctatttggc tggcaatata tgttttacag
     6481 tacgttgtct attttcgtca gtggaaaagt aactgatgtc gtttatacta aacaaaagaa
     6541 gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta gtaaatgaaa tccaaaactg
     6601 tatgcgtaga gggatcacga tcatcaatga agcagaaggt gcttataagc atgataagca
     6661 gacagtattg atgacgatcg ttacacgata cgaactgcct ctacttagag aagcaatgaa
     6721 agcttctgat ccacaagctt ttgtaagtat tgcagaaaac gtccagatac ttggtcgttt
     6781 ctatgaggat ggcatttaga tcgagtttga caaaaattca tgaaatactg ctgtagacag
     6841 acgttcgttt gtctgcagtt tttttctacc atagtagaat cagcaaagaa aaactgcttt
     6901 tttatttgaa attttcttaa agttgaaaaa aagcatgttt atttttttaa atttatggga
     6961 tgatagaaaa aaatagtaga gaaaggacac gattctactt gctattttca cttaataccc
     7021 gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa
     7081 ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta gctgtggtgg
     7141 gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg ggcgttccga
     7201 tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa tgggaacaaa
     7261 atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc tatccggcac
     7321 ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga gggctgttaa
     7381 acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg tggcaaatca
     7441 ataacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg catatctggt
     7501 ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta ttgttgatgg
     7561 tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc ttcctctccg
     7621 ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt tattacggta
     7681 ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt tggccaagta
     7741 cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa
     7801 tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac aatttcatct
     7861 attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca gtgacagcac
     7921 accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg attggcaaac
     7981 gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa gcaaaggtga
     8041 ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg attctaatca
     8101 tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat tatggtcgta
     8161 cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc aaaccatggg
     8221 ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa
     8281 acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag
     8341 cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa gcgatccttg
     8401 ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa ctaacggcat
     8461 ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat ccaaaagtag
     8521 tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt gaaaaattaa
     8581 aggaacaaga tttgttgaaa gacactgttc tgatcggact aggaacgaat ggctcattct
     8641 cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta tactgggtca
     8701 atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga
     8761 cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat gaccatagtg
     8821 actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat tacattcact
     8881 tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag acaaacgtga
     8941 cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg aagttttttt
     9001 atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt gagtgtgtct
     9061 gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct gcttttgttg
     9121 cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt cttttttgaa
     9181 gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta gaaagatcgg
     9241 ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa taatgttcat
     9301 ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt gtaagtgatc
     9361 aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc aactaacaaa
     9421 atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg tgcaattcga
     9481 tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac catagtaaag
     9541 cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa cgtcgtatga
     9601 atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt tggcagccct
     9661 ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac gacagataag
     9721 ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt aagcgcgata
     9781 ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat agccgcctgg
     9841 gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc aacaatcttc
     9901 ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga aaaagaaaac
     9961 actttggaac tttggataat cttgatatct tctgcgtata attggtcgat tcgttcgtct
    10021 tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta tactattttt
    10081 attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact atgaccataa
    10141 gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc gagtactatt
    10201 agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc aagatgagaa
    10261 ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg cggtagctgc
    10321 agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg
    10381 atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag gaccaagcgt
    10441 cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca tgtttcccag
    10501 tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca gaatgtctaa
    10561 agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc tgttcaagcg
    10621 tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca agaaaacgga
    10681 taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca aattggacaa
    10741 agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata agtttatgcg
    10801 tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg
    10861 agagacgtca agtctgccct ctttttgcgt tccaatatgt aaaatagaag acaggtgtca
    10921 tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga ttgaatgcgt
    10981 tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga cttgattgaa
    11041 gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa agtagaggca
    11101 atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc cgtagaatta
    11161 tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt tgctatatca
    11221 ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag ttattcgaac
    11281 tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa aaacgtacaa
    11341 tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga agatgacaat
    11401 atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga gctatttgat
    11461 tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt aaaatctgat
    11521 tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact acttacaccg
    11581 tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca ggataatgct
    11641 cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta
    11701 tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga acccctgcat
    11761 gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc
    11821 gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat cccgaatatg
    11881 atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc aaacggtcga
    11941 gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca aaaattagca
    12001 gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga aaaatggctc
    12061 ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta
    12121 caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg tttgacagaa
    12181 gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat ttttgatggg
    12241 atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa agagatgcgt
    12301 tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga acatcagcag
    12361 atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa aggaaacgga
    12421 acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg aaaagaaata
    12481 aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat gaatctcgat
    12541 gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa ttttttggtt
    12601 tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca atttttccaa
    12661 cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt ggatcattta
    12721 gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca
    12781 gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact cgaaaaacaa
    12841 atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca actagaaaat
    12901 tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat attaataaac
    12961 aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg atggattggg
    13021 tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga aataaaagtt
    13081 gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc gatgactacg
    13141 cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc
    13201 aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta
    13261 cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga agaattgaat
    13321 gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa
    13381 aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca gttaaaatat
    13441 ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca
    13501 cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag tcagctattg
    13561 aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca gcaaattgct
    13621 cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc aacggaacac
    13681 cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa agtagaacaa
    13741 caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc caaaagtgaa
    13801 gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat catggcaacg
    13861 aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct
    13921 gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc tttagataga
    13981 cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca acaagcagaa
    14041 aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc
    14101 cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac ggatagtaac
    14161 tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc gaatcaacta
    14221 gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct tcggacacag
    14281 cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa
    14341 agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga aatctgtgca
    14401 tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc ttatcatgaa
    14461 gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct aactgagaca
    14521 ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat gatcgaaata
    14581 aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa aatctacgat
    14641 gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac gcaattacag
    14701 cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga acggtatgtg
    14761 cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca acgtttaacg
    14821 aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg gaaaaccgga
    14881 ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca taccctgtct
    14941 ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga tgtgatccag
    15001 acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt tgggtcatta
    15061 gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga
    15121 atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca acaaataaga
    15181 attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata aaaagggaga
    15241 ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg tcaatacttg
    15301 caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa
    15361 gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga gatagattac
    15421 tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc tgtggaactt
    15481 tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat gctagaaccg
    15541 gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa
    15601 aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac gattgagact
    15661 aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg ttcattggca
    15721 tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc ttacacagaa
    15781 aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg ggcgtttcaa
    15841 aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca tcaattatta
    15901 ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct tctcccagta
    15961 gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca tctgcagata
    16021 aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt atcattcaaa
    16081 gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga ttttttcttt
    16141 atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa atatcaacaa
    16201 acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga agtagtgttc
    16261 aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc agagatcggc
    16321 gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt cgaatttatt
    16381 ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt ctatggcgaa
    16441 gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt tcaggtgtta
    16501 cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg ctaccagaaa
    16561 aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc ctttttccgc
    16621 acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa attgcgagag
    16681 ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac cggttcatgg
    16741 ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca tgtcctgcaa
    16801 agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct ttcttttgat
    16861 tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc gatacgtaca
    16921 gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa tcaattggaa
    16981 aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct cattcatcca
    17041 gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag
    17101 caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat tttagccgat
    17161 gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga aaaagaagaa
    17221 cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa ctggcaggca
    17281 gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa
    17341 cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc cagcttaaga
    17401 caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga tgaggcacaa
    17461 atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc tgtacctcag
    17521 cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg gtcattgttt
    17581 gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact tgccgcagaa
    17641 gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac tgtgttgaaa
    17701 gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga acaaaaaact
    17761 gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga ttctgaagca
    17821 ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca aatttgctgc
    17881 gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga acaagtaaaa
    17941 gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc tcaatttaca
    18001 gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac attttattta
    18061 agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa tgctggcgaa
    18121 aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct cacgggagca
    18181 gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca agctgctgga
    18241 cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga
    18301 acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg
    18361 atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg catgatttta
    18421 agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag
    18481 cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc tgggattcca
    18541 ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt tatacaaaga
    18601 ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg caaattggag
    18661 tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta gcactttttg
    18721 cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct ggaagcatat
    18781 gaaattcttt ctcagctagc tgaaaaatct ctttttctgg tttttggaaa cctgttgctt
    18841 gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt
    18901 ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc ttttccttca
    18961 agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt tcttcctcgt
    19021 aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct aaatctttta
    19081 gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct gccattactt
    19141 ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc atatctgcat
    19201 ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc tgatcataca
    19261 tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc tttcggtttt
    19321 cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta aaaaagctga
    19381 taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt ttttatattt
    19441 cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca aattaatcat
    19501 tatggtagaa gtcgattgat ttattttaat gcaatctctg tggaattttc gaaagattta
    19561 tttttttaat acagtctcaa tataactatt caaacataaa ataacgttat ttgatttatt
    19621 tgtttatagt aaattttatt tagtaatcat tgctttaaaa tactacgctt atataacaga
    19681 gaaaataagc gttataataa agaacagtat tatttaaaat gacagaaaac attactatta
    19741 aagaaataaa aaacataaaa aatcttaaga aatcggtttc atttagcttt tgagataaaa
    19801 taatagtaca gttttatacg ctgtttcata tgtttagaaa atttgaatta gatttgaaaa
    19861 ctctaatttt taaatgataa aaaaagcgag aatgctggtt ttactgtata ttaaccttag
    19921 ataactttca tttttttaaa aaaattatga aaaaaaggta gctatttaaa agttgattat
    19981 gtataataga aacagatggg agattattat gatcttctgt tacaaaaaag tgattcttat
    20041 cacgaaaagg agtggtgcac gttgttaaca ttatatactt ccccaagttg tacttcctgc
    20101 agaaaggctc gtgcgtggtt gcaagaacat cagattccat ttgttgaacg taatattttt
    20161 tcagaaccat tgaacagttc tgaattaaaa gcgattttac aaatgacaga agacggaaca
    20221 gaagaaatca tctcgacacg ttccaaagta ttccaaaaac tgaacatgga tttagatgat
    20281 ctgccgttac aagaattatt ggaacttgtg caaaacaatc ctggattgct tcgccgtcca
    20341 atcatgattg atgataaacg tctgcaagtt ggttttaatg aagatgaaat tcgtcggttc
    20401 ctaccgcgcg atgtacgaca attagaatta cgtcaagcac aattgatggc tggattatag
    20461 aaggataata cgaaacttgg aaaagactat tacaaagata gtttttccaa gtttttttgt
    20521 ttttacaaaa agtattcttg attagatgta taaaaaggta gtagaagatg atgattgcac
    20581 gaaaaataga gagggaaaat tagctgaaaa gtcggctata tagtattcgt ctgccaaaaa
    20641 tgttatagtt aaataagata gcttgaaagg ttgaaaaatc cgctggtttt attaaattcg
    20701 cttaacagcg agattttttt tctttacatt taagctgatt tcgctacaat gaatctatat
    20761 taaacattgt ggcaaagtga ggtgtagcaa aatggaaatg gaacatatta atgaaaatac
    20821 gatccgtgta ctaattggtc atgaagattt agaggaacgg ggcgtttcat ttttggattt
    20881 acttggcaat cacaaagaaa tcgaaaattt cttttatagt attttggaag aagttgatat
    20941 cgaagaggag ttccgaggaa gcgaagctgt cactttccaa gttttgccaa aaggagacgg
    21001 attagaactg tttatcagta aaaatcttcc ggatgaagaa atttctcaat cagaagattc
    21061 tactgttgat tcagaagatg tgagtgactt tcttcgtcag cagattattg gcagcgatcc
    21121 ggaggaagaa ttatctggaa gcgccctaga agaacaggaa agacaagcaa cttttgcttt
    21181 tgaagatttt gaacaagtga tccaacttgc aacagatgtc gaactagagt ctgcttggac
    21241 tgatttgtat ttattcgaag agcgctatta cctagtcgtt cacttctgga tggaaaatct
    21301 caaccaagcg gatgtagaga atcagattgc tcgaattttg gagtttgctg aaaagagcga
    21361 tcgtacacca gaaacattga atgaatatgg acaatgtctg atggcgcgta atgctatcga
    21421 gcgaacgaaa ttttattttg tataaatcag cattatagta agagactgtg acaaaaattg
    21481 tgtcgcagtc tttttttcgt ataaatgtgt tcaaaagctt gcatctgcgg taacaagccc
    21541 aaccaaacga aaaatattgg gaactatttt tgtttagctg ttcttattac ttgatgcaga
    21601 atagcttttt cacgccctct tttttcgtgt aaataaattc ttagacgtaa ataagttcag
    21661 agggagaaaa atgctgcttg caatgaatga atataataag tgctgttttg ctaaacatgc
    21721 agaaaaagga aagaaatatt attgtcgtgg ctgtcgaaaa agagtgattt tgaaaaaggg
    21781 aaagaaaaaa tgtgctcatt ttgcccatca aaaatcagat aactgttcgg tctttagtga
    21841 aagagaatca aaagagcact tacagttaaa ggaatgcttc atggattggc tgggacagtc
    21901 agtagaacca gtatttttgg aagcctattt gccgaggttg cggcaaaggc cggatatttt
    21961 gttggcgaat ctagcaatag aaatccaatg ttctagactg tctcaccaga gatttattga
    22021 aagaacccaa aattatttga ataacagtta ccaagtatgg tggatacttg gtaattcttt
    22081 tcttggacaa tcgcaattct cattgataga aaaaagttgc tgctactata atagaaaaag
    22141 aggagtgcat tgctggaaag ctgatctcaa gaaacaaaag ctttatttgt atcatcatat
    22201 tacagagact gtatcagggc atatttcctt ttgcagtagc tgctggactt tttctagtag
    22261 ggaccttaaa gaaatattca ctagtaacga aattaaaatg aaccaaatga aaaaaataga
    22321 aaaagtgagt gaagatggga agaaatggct gacacgccaa cttattcaca agcaaaagaa
    22381 tacagtatcc atacaagaac aatgctactt gcgtcataaa catcttttgc atctatcatc
    22441 gtggatctat caaaaaagtc ggtttttctt ttatttacaa gaacaggttt ttctttatcg
    22501 aatgttgtac gaagaagcct tggaaaacca gaaagttcct gatttcaact catggctttg
    22561 ccaaataaaa gaacataaaa gggaatggct ttttcctatg attgatgaag agatggtcta
    22621 tcagcagttt tttaacgaat gcatacactt gtcttctttg aaaaaatagt aagtataaaa
    22681 ataggctagg aatcggtttt ctatgtaaaa caagatttag ttatgctaaa attgatggaa
    22741 gcagacacat gaatgagtag aaaagttaga tgctgtgaca tgtggatgtt agactatgat
    22801 ctattttgat agatagagct aagggaagaa aaggagtgga tttcatggaa gtaaagcagt
    22861 tgccaaaacg agaagaattg cctgaaaatt taacttggga cttgaccaag atcttttcaa
    22921 gcgaccaaga gtttgatgag aaatatttgg aattatcaga agagttaaaa caatctgaaa
    22981 aacacaaagg aacacttgat caaggcgctt ctcaattttt aaatgcgatt gaattcgtat
    23041 tgagggttta tcgccaaact gaagtcattt atgtatatgc gcaccttaaa aacgatcaag
    23101 acactggaaa tacagattac caagcgcttt atgcaagagc aagcagtctg ttttcgaaag
    23161 ttagtgaagc cgtttcctgg tttgaaccag aaatattgca attgtcagat gaccagattt
    23221 ggcaatattt caaagaagaa ccaaaattgg aagtctatcg ccattatatc cagcaaatag
    23281 tagataatcg agcccatgtc ttatctgctg agcaggaatc tcttcttgct ggagcaggtg
    23341 aaatctttga tgcttcaagt gatacatttg ctgttttgaa taatgcagat ctagtttttc
    23401 caacgattga aggagaaaat ggtgaaatag tccaattatc tcatggcgtg tatggtcagt
    23461 tgctagaaag cacggatcga agggtgcgcg aagcagcatt taaggggttg tacagtgttt
    23521 acgaacaatt tagaaataca tttgcttcta ctttaggcac acatataaaa ggacataatt
    23581 ttaaagcgaa agtccgtaat tacagctctg ccagagaagc gtctttgagc aataatcata
    23641 ttcctgaaag tgtatacgat actttggtag acgtggtaaa caagcatttg cctttgttac
    23701 atcgatacat ggaattacgg aaacgtttat tagaagtgga aaaactgcac atgtatgatc
    23761 tttatacacc ggtcttaggg gaagctccaa ttacctttac gtacgaagaa gcaaaagaaa
    23821 aagctttaga agcactgaaa ccaatgggtg aagaatacat ggccatcgta gaaaaagcat
    23881 tctctgaacg ttggatcgat gttgtcgaaa ataaagggaa acgaagcggt gcttattctt
    23941 cgggaagcta tgacacaaat ccatatattt tattgaattg gcatgatacg ctggatcagc
    24001 tatttacgct tgtccacgaa atgggacata gtgttcatag ttatttcact cgttcgaacc
    24061 agccttatgt gtacggcgac tactccatct ttttagcaga aattgcttcg acaacgaatg
    24121 aaaatatcct aacggagtat ttattggaaa cagaaaaaga ccctcgtgta cgggcttatg
    24181 tactcaacca ttacttggat gggtttaaag gaacagtttt ccgccagaca caattcgctg
    24241 aattcgagca tttcatgcat acggaagatg aaaaaggtgt gccattgact agtgaatacc
    24301 taagtgatag ttatggtaaa ttgaatgcaa aatattatgg tccagcagtc gaagaagacc
    24361 cagaaattaa attcgaatgg tcacggatac cgcattttta ttataattac tatgttttcc
    24421 aatactcgac tggcttttct gctgcttcag cactggcgaa gaaaatacta aaccaggaac
    24481 cagaggcact agagaactac ttggcttacc taaaagcagg taacagcgat tatcctgtgg
    24541 aagtaatgaa aaaagcagga gttgatatga cacaggctgc atatattgaa gatgcaatgt
    24601 cgatgtttga acaacgtcta aatgaattag aagaattgat cgatcgttta taaggctttc
    24661 tatttttgat tttctcgaat agaaacgaaa aaaatacgtc ggctctcttg taagggagtc
    24721 gacgtatttt ttta
//
LOCUS       DANKMN010000047        24258 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000047 DANKMN010000000
VERSION     DANKMN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="PW224_001759"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="PW224_001759"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL2908447.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="PW224_001760"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="PW224_001760"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL2908448.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="PW224_001761"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="PW224_001761"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL2908449.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="PW224_001762"
     CDS             complement(3374..4681)
                     /locus_tag="PW224_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL2908450.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="PW224_001763"
     CDS             complement(4684..5265)
                     /locus_tag="PW224_001763"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL2908451.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="PW224_001764"
     CDS             complement(5837..7129)
                     /locus_tag="PW224_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2908452.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="PW224_001765"
     CDS             complement(7126..7749)
                     /locus_tag="PW224_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908453.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="PW224_001766"
     CDS             complement(7752..8588)
                     /locus_tag="PW224_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2908454.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="PW224_001767"
     CDS             complement(8605..9492)
                     /locus_tag="PW224_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2908455.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="PW224_001768"
     CDS             complement(9508..10791)
                     /locus_tag="PW224_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2908456.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="PW224_001769"
     CDS             complement(11474..12157)
                     /locus_tag="PW224_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2908457.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="PW224_001770"
     CDS             complement(12175..12792)
                     /locus_tag="PW224_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2908458.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="PW224_001771"
     CDS             complement(12802..13626)
                     /locus_tag="PW224_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL2908459.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="PW224_001772"
     CDS             complement(13619..14179)
                     /locus_tag="PW224_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908460.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="PW224_001773"
     CDS             14353..15309
                     /locus_tag="PW224_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2908461.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="PW224_001774"
     CDS             complement(15614..16570)
                     /locus_tag="PW224_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL2908462.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="PW224_001775"
     CDS             16674..17276
                     /locus_tag="PW224_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2908463.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="PW224_001776"
     CDS             complement(17364..18863)
                     /locus_tag="PW224_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL2908464.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="PW224_001777"
     CDS             complement(18879..19403)
                     /locus_tag="PW224_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL2908465.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="PW224_001778"
     CDS             19701..20450
                     /locus_tag="PW224_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2908466.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="PW224_001779"
     CDS             20441..21226
                     /locus_tag="PW224_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL2908467.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="PW224_001780"
     CDS             21195..21911
                     /locus_tag="PW224_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2908468.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23047)
                     /locus_tag="PW224_001781"
     CDS             complement(22013..23047)
                     /locus_tag="PW224_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL2908469.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23051..24109)
                     /locus_tag="PW224_001782"
     CDS             complement(23051..24109)
                     /locus_tag="PW224_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL2908470.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7623 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgatt
//
LOCUS       DANKMN010000048        24257 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000048 DANKMN010000000
VERSION     DANKMN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            139..996
                     /locus_tag="PW224_001783"
     CDS             139..996
                     /locus_tag="PW224_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL2908471.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1111..2031
                     /gene="pstC"
                     /locus_tag="PW224_001784"
     CDS             1111..2031
                     /gene="pstC"
                     /locus_tag="PW224_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL2908472.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2031..2915
                     /gene="pstA"
                     /locus_tag="PW224_001785"
     CDS             2031..2915
                     /gene="pstA"
                     /locus_tag="PW224_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL2908473.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2930..3736
                     /gene="pstB"
                     /locus_tag="PW224_001786"
     CDS             2930..3736
                     /gene="pstB"
                     /locus_tag="PW224_001786"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2908474.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3752..4510
                     /locus_tag="PW224_001787"
     CDS             3752..4510
                     /locus_tag="PW224_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2908475.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4523..5200
                     /gene="phoU"
                     /locus_tag="PW224_001788"
     CDS             4523..5200
                     /gene="phoU"
                     /locus_tag="PW224_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL2908476.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5438..6469
                     /locus_tag="PW224_001789"
     CDS             5438..6469
                     /locus_tag="PW224_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2908477.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSISIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6466..7233
                     /locus_tag="PW224_001790"
     CDS             6466..7233
                     /locus_tag="PW224_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2908478.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7333..8901
                     /gene="liaX"
                     /locus_tag="PW224_001791"
     CDS             7333..8901
                     /gene="liaX"
                     /locus_tag="PW224_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL2908479.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9198..9518
                     /locus_tag="PW224_001792"
     CDS             9198..9518
                     /locus_tag="PW224_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL2908480.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9518..9868
                     /locus_tag="PW224_001793"
     CDS             9518..9868
                     /locus_tag="PW224_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2908481.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10035..10973
                     /locus_tag="PW224_001794"
     CDS             10035..10973
                     /locus_tag="PW224_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL2908482.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10988..11818
                     /locus_tag="PW224_001795"
     CDS             10988..11818
                     /locus_tag="PW224_001795"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2908483.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11862..12887
                     /locus_tag="PW224_001796"
     CDS             11862..12887
                     /locus_tag="PW224_001796"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2908484.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12902..13840
                     /gene="galU"
                     /locus_tag="PW224_001797"
     CDS             12902..13840
                     /gene="galU"
                     /locus_tag="PW224_001797"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2908485.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13965..14405
                     /locus_tag="PW224_001798"
     CDS             13965..14405
                     /locus_tag="PW224_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL2908486.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14408..15010
                     /locus_tag="PW224_001799"
     CDS             14408..15010
                     /locus_tag="PW224_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2908487.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15078..16184)
                     /locus_tag="PW224_001800"
     CDS             complement(15078..16184)
                     /locus_tag="PW224_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2908488.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16394..17395
                     /gene="ccpA"
                     /locus_tag="PW224_001801"
     CDS             16394..17395
                     /gene="ccpA"
                     /locus_tag="PW224_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL2908489.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17731..20076)
                     /locus_tag="PW224_001802"
     CDS             complement(17731..20076)
                     /locus_tag="PW224_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2908490.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20355..21257
                     /locus_tag="PW224_001803"
     CDS             20355..21257
                     /locus_tag="PW224_001803"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL2908491.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21470..23245
                     /locus_tag="PW224_001804"
     CDS             21470..23245
                     /locus_tag="PW224_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL2908492.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23502..>24257
                     /locus_tag="PW224_001805"
     CDS             23502..>24257
                     /locus_tag="PW224_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2908493.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         7748 a         4334 c         5089 g         7086 t
ORIGIN      
        1 atagcataga aacttttaat gagatagttt tttttacaat tatgagaatt tattttgtta
       61 tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag
      121 gattaaagga gatttctaat gaaaaaactt aaattattct taccaataat aggattagcc
      181 attcttttat caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc
      241 tcttctgctt tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct
      301 ggaaaattca tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca
      361 ggagcagtcg acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc
      421 tcagcattag tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat
      481 attggcgtaa aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa
      541 aactggagtg aagtcggtgg aaaagatcag ccagtagtta tattgaaccg cgcttcagga
      601 agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca
      661 caagaacaag attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc
      721 agctatgttg ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta
      781 gaaccgacag atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg
      841 tacacaagag atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat
      901 gaagtacaag ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc
      961 gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt
     1021 agctaaactt ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt
     1081 atgttaaacc tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca
     1141 aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg
     1201 ctgatcgtgt tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc
     1261 ttttttactg ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc
     1321 caaatcggag caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt
     1381 attgtgacct ttttatctgc tatcgttgcc acgccatttg ccattggtgc agcagtattc
     1441 atgacggaga tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt
     1501 gtaggtattc cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc
     1561 agaagtattt ttggcgggac agggtttggt atcttagctg gtacatttgt gttgttcgtt
     1621 atgatcttgc cgactgtcac aacgatgact gttgatgcat taaaggcagt gccacggcac
     1681 tatcgggaag cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg
     1741 ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt
     1801 ggtgaagcct tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggactt
     1861 actactcctg cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg
     1921 ggaacagttg aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta
     1981 ttctttaata tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa
     2041 aacgctcaga taaaattgct acaggtgttt tatatatagt ttcggggatc atcgtattga
     2101 ttttagcagc tttactactt tatatcttag ttcggggaat accgcatatt tcatggtcct
     2161 tcttgaccga accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta
     2221 actcgattta tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg
     2281 gtatctatct ttcagaatat gctggaaaaa actggctaac agatgtgatt cgcacgtcga
     2341 ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg
     2401 ttattcaatt ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca
     2461 acttgcctct tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac
     2521 gagaagcagg attggcctta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc
     2581 cagaagcgac accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg
     2641 aagcagctgc attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact
     2701 ggaatccatt aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg
     2761 tccatatttg gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg
     2821 gcgcttctgc cgtgttgatc attgctgttt tactgttcaa cttcggcgct cgtgcgattg
     2881 gcaaacgatt ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata
     2941 taactggaat gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac
     3001 ggatgatcta catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt
     3061 tgaaaaaaat aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct
     3121 tcgttcgttg aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat
     3181 gtatcaaggc gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat
     3241 tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt
     3301 tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag
     3361 cttgaaacaa gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc
     3421 tttatctggc gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga
     3481 tatcttgctg ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga
     3541 agaaacactt gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca
     3601 acaagcagct cgtatcagcg actatactgc ctttttctat ctaggaaaag taatggaata
     3661 tgacaaaact aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc
     3721 cggtcatttt ggttaagacg gaggcctcaa catgacaaaa gaaattattt catctaaaga
     3781 tcttcatctg tattatggaa agaaagaagc actaaaaggg atcaatctaa gtttcaatca
     3841 aggggaaatc actgcgatga tcggtccttc tggttgcggg aaatccactt atctgcgttc
     3901 tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc accggaagtg ttttatataa
     3961 agacaaagac atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat
     4021 ggtattccag cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt
     4081 gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa
     4141 ggcagcgtct gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc
     4201 cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat
     4261 cttattggat gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat
     4321 gctgttagaa ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc
     4381 ttcacgtatt tcagataaga cggcattctt tttagatggt cacttgatcg aatacaacga
     4441 tacgaagaag atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg
     4501 atttggataa ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct
     4561 ccataatcag ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg
     4621 tgcatacatc aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat
     4681 caataacatg gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc
     4741 agttacaact gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg
     4801 tatggccgat catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag
     4861 aataccggaa atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga
     4921 caacgtccta gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat
     4981 ggatcaaaga gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca
     5041 ggaaaatcct gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga
     5101 acggatcgga gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa
     5161 aatcactgag ttaaatacga accacaatga gattttctaa actgaagctg tgattatgcc
     5221 gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag
     5281 taataagaac agtcaaacaa aaaatagctc ctcatatttt tcgtttggct ggacttatta
     5341 ccgcagatgc aagcttttga acaccgttta ttcgggaaag ggtttgtgac aaaacatgtc
     5401 acaaaccctt ttcatcattt catagaaagg ataaagaatg caaaaaaaaa ttagcatcat
     5461 cactatattt tttatttgtg tgcttacttc atgggttatt cagcatcaag ctgctttata
     5521 tgaagtacct gtcatcgaga tcgaaaaagt acgaataaaa gaggatcatc aattgattac
     5581 aggaagactg atgaacacgg atcaaaaagg agaatcgatt tatttatcgg ttccttatca
     5641 aaaaaatcaa atagaaacag caaatataaa agcgagacaa atgtatctag tccaaatgca
     5701 tggagatgaa tggcagctag tagcaaaaaa aaatgatggc tggcttttct tcttcacaag
     5761 tatctccatt ggcgccatgt tgcttattgg cggaaaacag ggtctatttg ctttaggaag
     5821 tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg
     5881 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
     5941 ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
     6001 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
     6061 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
     6121 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
     6181 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
     6241 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
     6301 agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
     6361 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
     6421 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
     6481 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
     6541 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
     6601 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
     6661 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
     6721 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcgat ccaaggtttt
     6781 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
     6841 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgctagtgta
     6901 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
     6961 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
     7021 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
     7081 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
     7141 accacattgt ctgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
     7201 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
     7261 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
     7321 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
     7381 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
     7441 ataaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
     7501 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
     7561 agagaaaggc aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
     7621 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
     7681 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
     7741 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
     7801 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
     7861 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
     7921 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
     7981 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
     8041 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
     8101 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
     8161 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
     8221 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
     8281 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
     8341 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
     8401 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
     8461 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
     8521 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
     8581 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
     8641 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
     8701 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
     8761 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
     8821 cgtgtttcag atggtgaaat cacgcaagtg caactttcaa cgacaacagg aagtatctat
     8881 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
     8941 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgataggct
     9001 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
     9061 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
     9121 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
     9181 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atccaataaa aatgttgttt
     9241 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
     9301 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aatcatttta tacattttat
     9361 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
     9421 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
     9481 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
     9541 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
     9601 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
     9661 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
     9721 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
     9781 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
     9841 tgtcagtgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
     9901 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
     9961 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
    10021 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
    10081 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    10141 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    10201 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    10261 tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccgg
    10321 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    10381 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    10441 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    10501 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggt catcgattga
    10561 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    10621 ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    10681 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    10741 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    10801 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    10861 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    10921 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    10981 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    11041 ctggtatgca ttgatcatcg tatcaggtat cattatcgcc atgtggctta gcagcagaga
    11101 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    11161 tatctccatt attggtgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    11221 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    11281 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    11341 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    11401 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    11461 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    11521 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    11581 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    11641 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    11701 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    11761 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    11821 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    11881 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    11941 acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga
    12001 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    12061 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    12121 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    12181 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    12241 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcga
    12301 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    12361 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    12421 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    12481 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    12541 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga gtaggcgatc
    12601 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    12661 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    12721 ctactcaagc agcgatggaa ctagcccaaa taatggaaat cgaaatgccc atcactcaaa
    12781 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    12841 tacgtgatgg gaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    12901 tatgaaagtc aaaaaagcag ttattccagc ggcaggatta ggaacaagat ttttaccagc
    12961 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    13021 cgttgaagaa gcgttgaaat caggaatcga agacatcttg atcgttacag gaaaagctaa
    13081 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    13141 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    13201 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    13261 aaatgaaccg tttgtcgtta tgctaggcga tgatatcatg gaagatgaag taccattgac
    13321 acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt
    13381 tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg
    13441 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    13501 agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca
    13561 accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca
    13621 acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt
    13681 tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga
    13741 gtatatcctg tctttaggta aaaaattaga agcagaagga agcaagatcg atcataatcc
    13801 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt
    13861 cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    13921 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    13981 ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt
    14041 tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga
    14101 cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttgctgg
    14161 tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    14221 tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca
    14281 tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa agcgacggtt gccggaaaag
    14341 tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    14401 aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag gaacagccgc
    14461 agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc
    14521 tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa
    14581 atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg
    14641 tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga
    14701 atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa
    14761 aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac
    14821 ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa
    14881 tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatga tgaaaatggc
    14941 aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac
    15001 aaatcactaa aatcactaaa atcacaaatt tatagaataa aaaggtggga gcggactagc
    15061 tctcaccttt tttactctta tttttctaaa actagtaatt cttttggtgt agtcgtgaat
    15121 ggcacacagc catcgcttgt cacatataca cagtcttcga tacgtacccc tactttttca
    15181 ggaatataga ttcctggttc aagagagaag cacatccctt cttcgattac taagtcattt
    15241 ccttctacaa gggaagggaa ttcgtggacg gttgttccga tcccgtgacc taaacggtga
    15301 ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca
    15361 cctgctgtca cacctggacg gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg
    15421 ttgtatattt tttcttggaa gtcggttggt ttttgataag caattgtacg agtagcatca
    15481 ctgcaatagc cattccaaac aacgccaaga tcgaataaca caagttcatt cggtgaaatc
    15541 ttcgtgtttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg
    15601 tcgaaagaca tttgtgaaac accttgtttt ttcaattggt attcgatttc tgcaatgatc
    15661 gcttgttcag tcacgccttc tttcactgct ttaaagccaa tctcaaatgc aacatcggcc
    15721 catttaccag cttcgatcag tttgtctttt tcctctagag ttttgatcag ttgtagacgt
    15781 tggatgatgg aagctagatc agtgtaagaa tccgttcccg tcaagatatc agagatagca
    15841 tcgaagcgct cgacagtcaa ggcgttcttt tcaatcccta cattgccagg ctgaccatag
    15901 cgagcacgaa gttcttttcc gatgatctcc caagggtttt cgctgtctaa atagccacgg
    15961 acatcatacg cccaactact gttttgagca tcttcaactt ccaaagcagg agtgaaaagg
    16021 aaaggatctt gttcaagagg gataaaaaga gctagtacac gctcatgagg atcactttcg
    16081 tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc gacttggtgt
    16141 tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc
    16201 taaattaatt ttgtattcaa taattgtacc actgatttat taaaagacca acgttctaaa
    16261 aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttcttgagat
    16321 tatttgatat tctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa
    16381 taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa
    16441 tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac
    16501 aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaacg cagttgcgcg
    16561 tggcttggca agtaagaaaa caacaacagt tggggtaatc atccctgatg tcagcaatat
    16621 gttctttgct tctttagcac gtggtatcga tgatgtggca acaatgtaca aatacaatat
    16681 tatcttagca aattcagatg gtaacgacca aaaagaagtc aatgtattga ataacttact
    16741 ggctaagcaa gtggacggcg tgatcttcat gggtcaccat atcacagacg aaatccgtgg
    16801 agaattttca cgttcaaaaa ctcctgttgt attagctggc tctattgatc cagatgaaca
    16861 agtaggtagc gtaaacattg attatgtatc agcaacaaaa gatgcagtaa acaaattggc
    16921 aaaaagcggc aataaaaaaa tcgcttttgt cagtggtgcg ttgatcgatc ctatcaatgg
    16981 acaaaatcgt ctgaaaggct ataaagaagc attaagcgaa aataacctac catatagcga
    17041 aggattgatc tttgaagcac aatacaactt caaagatggt ctatctctag tcgatcggat
    17101 ccataatagc ggtgcaacag ctgtctatgt ttcagatgat gaattagcca tcggtatttt
    17161 ggatggttta ttagaccgtg gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa
    17221 caattcacta ctgacagaag tagctcgtcc tcgtttaaca agtatcacac aaccattata
    17281 cgatttaggg gcggtatcta tgcgcctatt gacgaaaatg atgaataaag aagaagtcga
    17341 agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatctacaa aataataaaa
    17401 ggacaaccat tcagaggttg tgagcctgcc gtttaactct gcgttacaag gagcaatttc
    17461 aaaaaacagc atttcatgtt tgatgaatat tgtgactcgt gattcggagt tggcaggaga
    17521 acaccgttta ttcgggaaaa ggggttatga caaaattttg tttaatcctt tttttttgtc
    17581 ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata
    17641 gatgctgcat tatgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca
    17701 cagtccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg attctttggt
    17761 tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat agttttcatc
    17821 ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg cccatagcga
    17881 ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt ttccaggtgt
    17941 ttgaccagta aatccagata cttttttctt catcacatca gatgataatt cgaatttctc
    18001 atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat tggcaagata
    18061 attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt gccctgccca
    18121 gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt attcattggt
    18181 agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgagatggc
    18241 atctttgaat ggtgtagtga tttttgcatt gatcacactg cgcatcatgt cattcatgat
    18301 cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat aaattacatt
    18361 tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc cgccgtttgc
    18421 taaagcctga aatccattgg tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc
    18481 agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt agtgctcgta
    18541 agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat aagccgggat
    18601 attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc cttcgttagt
    18661 agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg cataatctga
    18721 tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa caagaactgg
    18781 tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt gattctgact
    18841 gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca tcaatacatt
    18901 tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat tttctacgcc
    18961 attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat atcctttatt
    19021 ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat cagacatatc
    19081 agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat tcatcacagt
    19141 ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt
    19201 aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt cacgatacat
    19261 actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt ttagcgcacc
    19321 tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga tataagcagc
    19381 ttgcggcagc gtcacatctt ttgcatctac accaaagatt cctcttgcag cttcctggac
    19441 tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca aatacgtagc
    19501 aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga tctcgtttgc
    19561 tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt gctgtgtcaa
    19621 agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc gtaaaacagc
    19681 tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga taattgcagt
    19741 tttcaataaa ggtgaaattt tatcagatga aatagtcgtg cgcatcagat ctgtctggat
    19801 ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa tgtttcctag
    19861 ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata
    19921 gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag taataaaaac
    19981 agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt ctttttttct
    20041 tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt
    20101 tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt ttttggcagg
    20161 aatttctttt ttaaaagagt taaaacgtga gaaaatgatc ttttttataa aagagttctt
    20221 aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga aaaatcaatc
    20281 attttctttg tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa
    20341 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
    20401 taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg
    20461 cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt
    20521 acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg
    20581 ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg
    20641 ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg
    20701 caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag
    20761 aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa
    20821 catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta
    20881 cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg
    20941 taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg
    21001 attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt tttggaacaa
    21061 tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa
    21121 tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca tatggaaaag
    21181 aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag ttattagcag
    21241 aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc
    21301 cttgtccttt tttgtttaag aaacaaagga gtgctttttg cagtcttttt catcatgcat
    21361 cttctccccc ctctttttaa atttagacaa acagggtata ctggtagaaa agtaaaaaaa
    21421 cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac
    21481 tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc
    21541 cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat
    21601 gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa
    21661 atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt
    21721 agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt tcagtatttt
    21781 tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt atcttctttt
    21841 aagtttctat ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg
    21901 aacgtatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt gtttactagc
    21961 tatttattac cgtaaatata gttccagcaa aaaaatgctg ccgtggtttt tagggctagc
    22021 tgtttctttt cctttctttt ggttcattcg cgaagattcc atctggcttc ttccttttac
    22081 tgtagtcgcg ttaggcatca gtttcgtcca gatcttgcta ggacaagact tttctttcaa
    22141 agaagcaaag aagttaaaag aacgaccatt tctcctttcg aaacaagaat ggctgaaact
    22201 ttcattgttt ttactccctc tagtcggttt ctccatcgtc tatcagtgga tcgaatataa
    22261 aaacgagaca acatatagta ttgccgccat caatgaccgt tcgagtggcg aatttggacg
    22321 tttgatgaaa cagctgatcc gaatagatga cgaaacgaac ttaaataaag aaaacagtga
    22381 gatatgggtc tcgcatgctg cattaaataa agcattagac gcatctccta catttcatgc
    22441 tgtttctact cgtattgacg aattgtatac gactcatgcg tggacccaag gtggaaaagt
    22501 aaaagaatta ccaggagata tcattttttg ggcattgcga gatgtggtaa gcgaatctgg
    22561 ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca atcaagaatt
    22621 agaagaagcc tatcaaaagg gaactatcaa aaagaaaaaa gaattctatc tgatgggatc
    22681 aggggatgga aagacgatga atgatctgcc aatcgtattt gcttttctga aatcaggata
    22741 tgcctatgca tggcgataca aagatttcca tcaagggggg aattatagtt tcggtacgca
    22801 agaacaagtc gaacaggccc aagaattatt gaatcttcca ctaatggata attggatcga
    22861 tagcagtgat ccgagaacac ctgatttcca gttgacaaaa acagcaaaag tcgcgaatgt
    22921 catgataaaa atttaccaag ttctggccat tcccgtgctg attgcttcaa tagcagggat
    22981 attacttatg atactaggaa ccgcatttgc aaaagctgat cgtgcctatt atagttcctc
    23041 tttgattgta gtcaccggat taatactgac acaattggtt tttcttttcg gcgtctcgtg
    23101 gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca ctggcgcagg
    23161 cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta aattgaatct
    23221 gaaaaaagct ttatcttcca aataaaaaca tactaagatt agaggagaac atcattgacg
    23281 aatgatgttc tcctctattt tctattttta gattatagtg aagagtgaaa gaaagttcga
    23341 acaaagcctt gaggactatg agtccctatc aaaaaccgaa cttctttact gtcattttag
    23401 aatcaggttt acgtatgttg gggccatgag cccgtgtata ggaaattgga atcgaaatga
    23461 agcagtaaag ttctttcaaa acttaaaaag gaggaaaaca aatgccttac attcttgcat
    23521 tagatgtttc aatgggacac agctacagtg tcctatacaa agacgacacc tgcttatttg
    23581 aagacgagat cgaacacact caaagaggat ttcatgcatt actcgaagag atccagcact
    23641 tgcctgaacg tccattgatt gtttttgaag caacggggat ttattccaga ccagtcgaaa
    23701 agttctttca agatcatcac ttttcttact gtttgttaaa cccgttagaa gcgaaaaaac
    23761 agatggaaga aaacacctta tgcagttgga aaacagataa atcggatgcc catcggctcg
    23821 cacagactca tcttaaaaat caacgtcagc caaaagaagt acaaagcaat ttttacttag
    23881 agatgcgcga tttagcccgt ttttatcaag aaattgagaa gaaaatcact cgtctgcgca
    23941 tggatttaca caactgcctt cagttgactt ttccagagct tgaacagttc tttttaaaca
    24001 ggctgacccc ttacgcattg atattgatcc gtctttttcc ccatccagat tttgtattgg
    24061 catctactcg gacaaaaata aaaaataaac tgatcaacga aacacggaaa aaaatctccg
    24121 cgaatcgtgc cgagcaaaag gcagatcaaa tcatccacta tgcacagtgt gcctatccag
    24181 ctgttgaaaa agacagtatc cactgtcaaa aaacgatcta ttacgctgaa cttctccaag
    24241 atttactcga acaaaaa
//
LOCUS       DANKMN010000049        23829 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000049 DANKMN010000000
VERSION     DANKMN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(54..788)
                     /locus_tag="PW224_001806"
     CDS             complement(54..788)
                     /locus_tag="PW224_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2908494.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="PW224_001807"
     CDS             1115..1525
                     /locus_tag="PW224_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL2908495.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="PW224_001808"
     CDS             1518..2219
                     /locus_tag="PW224_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL2908496.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="PW224_001809"
     CDS             2219..3805
                     /locus_tag="PW224_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908497.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="PW224_001810"
     CDS             3824..4045
                     /locus_tag="PW224_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL2908498.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="PW224_001811"
     CDS             4042..4803
                     /locus_tag="PW224_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL2908499.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="PW224_001812"
     CDS             5183..5692
                     /locus_tag="PW224_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2908500.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="PW224_001813"
     CDS             complement(5767..6306)
                     /locus_tag="PW224_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL2908501.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="PW224_001814"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="PW224_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL2908502.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="PW224_001815"
     CDS             7319..8170
                     /locus_tag="PW224_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL2908503.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="PW224_001816"
     CDS             8198..8833
                     /locus_tag="PW224_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL2908504.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="PW224_001817"
     CDS             8853..9626
                     /locus_tag="PW224_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2908505.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="PW224_001818"
     CDS             10034..10831
                     /locus_tag="PW224_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL2908506.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="PW224_001819"
     CDS             10809..11222
                     /locus_tag="PW224_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2908507.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="PW224_001820"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="PW224_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2908508.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="PW224_001821"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="PW224_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL2908509.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="PW224_001822"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="PW224_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL2908510.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="PW224_001823"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="PW224_001823"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL2908511.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="PW224_001824"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="PW224_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL2908512.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="PW224_001825"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="PW224_001825"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL2908513.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="PW224_001826"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="PW224_001826"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL2908514.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="PW224_001827"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="PW224_001827"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL2908515.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="PW224_001828"
     CDS             21906..22484
                     /locus_tag="PW224_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2908516.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="PW224_001829"
     CDS             22536..23018
                     /locus_tag="PW224_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL2908517.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="PW224_001830"
     CDS             23135..23509
                     /locus_tag="PW224_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908518.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6647 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagttt
//
LOCUS       DANKMN010000050        23653 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000050 DANKMN010000000
VERSION     DANKMN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..506)
                     /locus_tag="PW224_001831"
     CDS             complement(<1..506)
                     /locus_tag="PW224_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2908519.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="PW224_001832"
     CDS             779..3250
                     /locus_tag="PW224_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2908520.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="PW224_001833"
     CDS             complement(3353..5089)
                     /locus_tag="PW224_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL2908521.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="PW224_001834"
     CDS             complement(5086..5790)
                     /locus_tag="PW224_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2908522.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="PW224_001835"
     CDS             5921..6424
                     /locus_tag="PW224_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL2908523.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="PW224_001836"
     CDS             6384..6722
                     /locus_tag="PW224_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL2908524.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="PW224_001837"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="PW224_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL2908525.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="PW224_001838"
     CDS             8273..8851
                     /locus_tag="PW224_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL2908526.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="PW224_001839"
     CDS             8855..9376
                     /locus_tag="PW224_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2908527.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="PW224_001840"
     CDS             complement(9455..10009)
                     /locus_tag="PW224_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2908528.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="PW224_001841"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="PW224_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL2908529.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="PW224_001842"
     CDS             10549..10800
                     /locus_tag="PW224_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL2908530.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="PW224_001843"
     CDS             10925..11716
                     /locus_tag="PW224_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908531.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="PW224_001844"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="PW224_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL2908532.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="PW224_001845"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="PW224_001845"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL2908533.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="PW224_001846"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="PW224_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL2908534.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14014..14676
                     /locus_tag="PW224_001847"
     CDS             14014..14676
                     /locus_tag="PW224_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908535.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14755..17982
                     /gene="fss3"
                     /locus_tag="PW224_001848"
     CDS             14755..17982
                     /gene="fss3"
                     /locus_tag="PW224_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2908536.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18096..18410
                     /locus_tag="PW224_001849"
     CDS             18096..18410
                     /locus_tag="PW224_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2908537.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18423..18797
                     /locus_tag="PW224_001850"
     CDS             18423..18797
                     /locus_tag="PW224_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2908538.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18807..19151
                     /locus_tag="PW224_001851"
     CDS             18807..19151
                     /locus_tag="PW224_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2908539.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19222..20574
                     /locus_tag="PW224_001852"
     CDS             19222..20574
                     /locus_tag="PW224_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2908540.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20634..21776
                     /locus_tag="PW224_001853"
     CDS             20634..21776
                     /locus_tag="PW224_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL2908541.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21801..22055
                     /locus_tag="PW224_001854"
     CDS             21801..22055
                     /locus_tag="PW224_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908542.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22311..23495
                     /locus_tag="PW224_001855"
     CDS             22311..23495
                     /locus_tag="PW224_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2908543.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23492..23629
                     /locus_tag="PW224_001856"
     CDS             23492..23629
                     /locus_tag="PW224_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2908544.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7832 a         4218 c         5073 g         6530 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa
    13681 gtccttgtat atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc
    13741 tgttttggac catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg
    13801 actatcagtg tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata
    13861 cgaaaaatac caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg
    13921 tcagctatta gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg
    13981 cttctatcta aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt
    14041 tggaaaaatc aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac
    14101 ggaattacat ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca
    14161 agtatgtggt tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga
    14221 cgagaaaata ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata
    14281 gtagaagcct ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca
    14341 gtagggaaga atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca
    14401 ctttttaaag aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta
    14461 aaaaatgaaa taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa
    14521 ctagttcaat ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat
    14581 tgggaggaat tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag
    14641 agaaacaacc tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa
    14701 aattaaaatt tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa
    14761 aagccaagat ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt
    14821 ggtggatcgc ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta
    14881 actgtggaat tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt
    14941 cccatgtcag aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt
    15001 ttctgtattg agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat
    15061 ccattgcctg atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt
    15121 acggatgtgg atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac
    15181 acgcttcact caattaaacg ttctgatggt agtgcagtaa atatcgcagc cattgagtca
    15241 aaaatcaatc aagccattgc ggattatcaa aagaaaccaa gcttccataa ctccacagtg
    15301 aaaaccgtgt taggtcaatc gactactgtg acagatacga acggcttgaa tttatcggag
    15361 tttgatgaag ttgtcgagaa tatcgcaaat attgattatc gtgtgaacgg caatcaacta
    15421 gtgattactc caaatgccaa ttcaaaagag agtggtgaat taaccctaaa gaagtcagct
    15481 aatacaggaa caccggtagc ttataaaatg gctggtcaac aaactttgat ggcgggggca
    15541 attgataagc ccaataccta cacaatcaaa attgatgtgg aaactgaggg ttctttgaag
    15601 attaaaaaag tcgataaaga aacaggaaat gttgtaccgg gaaccgtttt ccatttagac
    15661 tttggaaaaa cattacctgc aaaagacgtg accaccgata aagaaggcat tgcgacattg
    15721 gatgagattc ctcatggcgc gaaagtaacc attactgaaa aatcagtgcc agctccttat
    15781 acgattgaca ctacaccaat caccgcaacc attaaatctg gtgagactat ttctgtgact
    15841 tcaaaaaata ctcgcgaaaa aggtcaaatc attttgaata aaatgggggt ggaaacagga
    15901 actgatcttt ggaacgacaa atactcatta gccggaaata cgtttgccat tcgcaaagac
    15961 agtccaaccg gtgaaattgt ccaagaaatg accacggatg aaaaaggtca tgcggaaaca
    16021 ccaaaagaga ttgccaatgc gttggaattg ggaacctatt atgtgaccga aaccaaagct
    16081 agtaatggct ttgtgaatac ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa
    16141 accgtggctc ttgttaccag taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc
    16201 actttgacaa aagaagacaa agataccggt aatgaaactc aaggtaaagc tgagtttaaa
    16261 ggagctgaat atactctctt tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct
    16321 tttaaaccag agttagtgaa gggaacgaaa gcttctgatg aaacggtgac tttgacttta
    16381 gatgaaaaga atcaagttgc tgttaaacac ttagcgatta atgagtatta ctggcaagaa
    16441 accaaagcac ctgaaggata taccttggat gaaacaaagt accctgtttc catcaaaaag
    16501 gtggatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacaagtc
    16561 attcgctttg gctttgattt ctttaaattt gctggatcag ctgctgggac tgccgaaact
    16621 ggcttcaatg atctgacctt taaagtatcg ccattggaag gaaccagtga gattacaggt
    16681 gccgaagatg aagcaattac agcttataac gagcaactag gttttgatgg ctatggcaag
    16741 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaggctcc agaaggattt
    16801 caaaaaatta cgccattaga aatccgttct acttttaaag aaaataaagc ggattacact
    16861 aagagtgaat acatttttac gattaccgaa aaaggacaaa aacaaccaat taaaacggtg
    16921 accgttcctt acgagaaact gacgaataaa gcattttctg ttagcttgaa tcgtttgatg
    16981 ctctatgatt tacctgaaga agaagatagc ttaacttctc ttgggacttg gaaagacgga
    17041 ggcaaagaat tgacagacct tgattctacc gagctagttg ataaattgag ctataacttg
    17101 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa
    17161 gctgcccaag aaaaagatga aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg
    17221 ttggcgaata aagagaaaac aggaacttgg aaaattttgc ataaattaac cgcagaacaa
    17281 gttttggata aaaccatcgt cttattcaac tatgtgtatg aaaacaagaa accctatgaa
    17341 gcaggtgatg atccagtagc gaaagatgct agcttgaaca atcaagccca aactgtcaat
    17401 tgtacggtgg aacgccatgt ttccatccaa acaaaagccc acctagaaga tggttcgcaa
    17461 acctttactc atggggatgt ggtggatatg ttcgatgatg tgtcgattac ccatgatgta
    17521 ctggatggct caaaagaagc ttttgaaaca attctgtatg cattacttcc ggataatacg
    17581 accaaagaaa tttggaaatc tggcaaaatt gattatgaag tgaatgacaa agaattcact
    17641 aaaaccgtac ttgcggaaaa agtggacaca agtaaatatc cagaaggtac aagttttacc
    17701 ttcaaagaaa ttaactatgg caaagacgga aacatcaatg ggaaacacaa tgaggatcta
    17761 aaagaaaaaa ctcaaacttt aacacctaaa aaagttccga caacaccaag tgcaccagaa
    17821 caaccggaaa taccaactgt tccaagtgac tctcaagaat ctagtcctac agtaaagaca
    17881 ttcccacaaa caggggagaa aaattctaat gttctgctgt ttattgggtt taccttgatc
    17941 tttgcgactg ctgggtatta tttctggaat cgccgaaact aaggtgatgt gatgaaacaa
    18001 ctaaatagga aaaaccaacg tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt
    18061 gtatccaaaa ctaaaaatga aaagaggaaa agaagatgga attgaaattt gttgtgccag
    18121 atatggccga aacctttgga aagataaggt atgccggcga aggcgaagtt ttaactgaag
    18181 gatatgggcg gaatactaca gtaattggtc gtagttatca tttgtattcc agtaagcaac
    18241 gagccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgatcaag
    18301 atcaaccact aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    18361 gtgggtttac cgactacgtg ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga
    18421 agatgagatt aacagaaggt attgttgtag attcaggact aacgtttggg aaattacgat
    18481 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacggtt agtaacgagg
    18541 taaaagaacg gacttataat ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta
    18601 tcccagcaaa tgtaccctta cgcgagtttg cctatgacgc agaggtagag ttggtcaatc
    18661 caatcatgga tacggtcgcc aattatgttt tccgagaagg aactactgtc aattggttca
    18721 ttaaggccga tgatttagtc ttgaaacgac aaccagatca gggaaattct acaaaccaaa
    18781 atgaaggaaa gaaataggtg aagcgtatgg aaattaatgt tgaacatatt ttagattgtc
    18841 tcgatcaata tggaaaaggt gagctaacgg aggaacaact cactaaagca ttaacctatg
    18901 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgtcgaag
    18961 aagattttga tgtactgaat tggttagggg aagaagagtc cttctttatg gttatcgaag
    19021 taaatgaaag tctatgtcga gaagccgaaa ccgtattgga agaaattggt gtggaaatgc
    19081 cggatgccat tgaagttttt ttaaagcaac tagtggaaac gaaacaactc cctatagcag
    19141 taaatgacta gcataactgc ttgaaattgg atcaatgaaa tcctatgaag cttttttcga
    19201 ttggaggtgg aaaccataaa aatgtacaaa gggcatcgta tacgggcagg agatcagcac
    19261 ttggtttatc actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc
    19321 ttttattttc aagagcttag acaatttgat atttctaagc tatcactttc tactatagaa
    19381 atcgttcgtt ctataaaaga tttaatctat ttgctaggaa gtctcgttct ttcaggaagc
    19441 acgatgttgc tttatataca cttttttcag gatcattggc gcagtttgtg gcatcgacaa
    19501 aagctagccc ggatgattct agaaaatcat tggtacgaag taaaacaaac tcagagtgaa
    19561 ggatttttca aagacctcaa tagtagtcga accaaagaga caatcagtta cttccccaaa
    19621 atctattacc gtatgaagga tggtttactt tctattcgcg ttcaaatttc actgggaaaa
    19681 tatcaagatc agcttttaaa gctggaaaag aagttagaaa gtggtctata ttgtgaacta
    19741 gtagaaaagg aactcaaaga ctcctatgtg gagtacactt tactgtatga tataattgcc
    19801 aatcgaattg ggatagacga agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat
    19861 caagtatggg cttatgattc tttacctcat atgttaatcg ctggcggcac aggtggtggg
    19921 aagacttact ttttactcac tatcatcgag gctttattaa aatctgatgc cgagcttttt
    19981 gttcttgatc ccaaaaatgc ggacttagcc gatttaggta cggtaatgcc tcatgtctat
    20041 tcccaaaagg aggaaatttc tgcttgtgtg gaagatttct atgagcgcat gatgactcgt
    20101 agcaaggcaa tgaaagaaat gcccaactac aaaacaggag agaattatgc gtttcttgga
    20161 cttccaccaa actttttaat ctttgatgaa tacgtggttt atatggaaat gttaacgacg
    20221 aaggaaagtg cggtgatttt gaataagctc aaacaaatcg tgatgttagg tcgtcaatct
    20281 ggtttctttc tgattctagc ttgtcaaaga ccggatgcga aatatttagg agacgggatt
    20341 cgcgatcaat ttaatttccg ggtggctttg ggtcgtatga gtgaactggg atattccatg
    20401 atgtttggcg aggttgataa aaaattcttt atgaagcgta tcaaaggtcg aggctatgta
    20461 gatacgggag ggagtgtgat tagtgagttt tatacgccac ttgtaccaaa aggatatgat
    20521 ttcttaaggg aaattagcga cattggtgga ttaaacgtta atacggaagg ataaaataat
    20581 agtatgtagc ctaggagtgt caagaatcct gggctttttt tacgaggtga tttatgacat
    20641 ttttagattt attcgctggg attggtggct tttgcttagg tatggaacaa gcgggtcacc
    20701 agtgtatcgg cttttgtgaa attgatgatt ttgctcgcca gagttataag gcaatccatg
    20761 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagacgag tttattcaat
    20821 ctctcggacc agtggatatc ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg
    20881 gaaagcggca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
    20941 ccgctattct ccaacctaag tttttattcc ttgagaacgt ccggggatta ctcaatcacg
    21001 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgatgtgg
    21061 aatggcaagt gcttaactca aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta
    21121 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga tcgtctccaa
    21181 catctgatca aaacatcaga aacttattaa atatcaaccc ttccaatcga ggaatggggg
    21241 gacaagtcta tgggtcagat gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa
    21301 tcgcattgcc ggtagatgat ggaatttctg tagcgggtat gttactggga aattttgaac
    21361 aaggcaatcg ggtttatgag ataactggaa cagcgcctac tttgtcaacg aaacaaggcg
    21421 gaacgaagat catgcttcgg aaaaacgaaa cagaatatca agaggtgaag ccagggaaca
    21481 gtgtgaacct ggcttttcct aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg
    21541 tgcatacctt attaacagga gaccaacaag cagtcgtcac ggatcagtat caaattcgaa
    21601 agttaacacc tagggagtgc tggcggcttc agggctttcc ggactgggct tttgatcgag
    21661 cttctcaagt caattcagat agtcagttgt ataagcaggc tggaaattct gtaacagttc
    21721 cggtcatttt tgatattgct agaagattaa aggaggtaaa aacagatgat ttatgatgag
    21781 ttgattgggg aatctactgg gtgattggca agattcagtc agatccggaa ttggaagaag
    21841 aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccct gatgatccgt
    21901 atgtaattga tgaggaaacg gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg
    21961 aacgaattgc ggactttgct aaagaacctg atatccgcca atacttgttt gagataaaag
    22021 cagagttgga aattgagggt attaccgcgg agtaacggcc aaataaacca ctgataggca
    22081 ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt gctgtgcctg tttatggacg
    22141 gcgttagccg gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa
    22201 aaataagcaa gcaacgataa ggaatggctg tcctatcaag gataataagc aatttgtgta
    22261 gatgttaact gatttgaaaa agttaacacc ttaaatttgg aggtgagaac ttggcacaaa
    22321 ggaatttaga ttatcggcta ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata
    22381 aattagctac gacttgtgga cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg
    22441 taaccgcatc gacaaaaacc atgaggaaaa tttttgacca actggaaagt ttcaatcccg
    22501 agttaccttt aacgttactg tttgactatg taaggattcg ctttcccaca acggatgcga
    22561 ggaaaatcat tcaagagatt ctccatttga aatttgatta tatgctccac gaagattacg
    22621 ccttttactc ctatcaagaa caatatgtta tgggagatat tgtggtgatg ttgtcacatg
    22681 aagaagataa aggcgtcctt ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt
    22741 ttttactcgc ccaaaagcgt agctggtatg actttttcga agattgtcta aaagccggtg
    22801 gcgtgatgaa acgcttagac ctggcaatca atgatcgagt tggcctgtta gatattcctg
    22861 atttaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct
    22921 atcgttccgg ggaactgttg aaacctgatg ataaagatgg tatgggcaat actttgtata
    22981 ttggcagtct taagagtgag gtctatttct gtttgtacga aaaagactat gagcaatata
    23041 tcaagttagg gattccttta gaccagaccg aaacgaaaaa tcgttttgaa atccgactga
    23101 aaaatgatcg agcttatcat gcaattcaag atttgttaaa aggtcgcagt attgaaagta
    23161 ccacattttc cattattaac cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc
    23221 gaactaactg gcccttgaat gaacaatggg ggcgcttcat tggacggaat cagaaggaaa
    23281 ttcaattaac ttctgaaccg aagccttata ccattgaacg aacgttgaac tggcttggac
    23341 gacaagtcgc ccctacgtgg aagatggcca aggagttgga tcggttgaat cagacaacgt
    23401 acattcagga tatggtaaat aatactcgac tatcagaccg gcacaaaaag attttggagc
    23461 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtga tggaaattgt
    23521 gaaagatatt ttattggtgt ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    23581 gcatgcaagt gcagcggcgg accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt
    23641 tcggtcaaaa ttt
//
LOCUS       DANKMN010000051        23039 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000051 DANKMN010000000
VERSION     DANKMN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..2569)
                     /locus_tag="PW224_001857"
     CDS             complement(<1..2569)
                     /locus_tag="PW224_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908545.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
     gene            complement(2791..3675)
                     /locus_tag="PW224_001858"
     CDS             complement(2791..3675)
                     /locus_tag="PW224_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2908546.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3770..4207)
                     /locus_tag="PW224_001859"
     CDS             complement(3770..4207)
                     /locus_tag="PW224_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908547.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4424..5335)
                     /locus_tag="PW224_001860"
     CDS             complement(4424..5335)
                     /locus_tag="PW224_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2908548.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5712..6350)
                     /locus_tag="PW224_001861"
     CDS             complement(5712..6350)
                     /locus_tag="PW224_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2908549.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6354..7124)
                     /locus_tag="PW224_001862"
     CDS             complement(6354..7124)
                     /locus_tag="PW224_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL2908550.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(7542..7958)
                     /locus_tag="PW224_001863"
     CDS             complement(7542..7958)
                     /locus_tag="PW224_001863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908551.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(8009..8390)
                     /locus_tag="PW224_001864"
                     /pseudo
     CDS             complement(8009..8390)
                     /locus_tag="PW224_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8516..9181
                     /locus_tag="PW224_001865"
     CDS             8516..9181
                     /locus_tag="PW224_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908552.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9578..11611)
                     /gene="brxL"
                     /locus_tag="PW224_001866"
     CDS             complement(9578..11611)
                     /gene="brxL"
                     /locus_tag="PW224_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HDL2908553.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(11729..14251)
                     /gene="pglZ"
                     /locus_tag="PW224_001867"
     CDS             complement(11729..14251)
                     /gene="pglZ"
                     /locus_tag="PW224_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HDL2908554.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(14367..17999)
                     /gene="pglX"
                     /locus_tag="PW224_001868"
     CDS             complement(14367..17999)
                     /gene="pglX"
                     /locus_tag="PW224_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HDL2908555.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(18063..21734)
                     /gene="brxC"
                     /locus_tag="PW224_001869"
     CDS             complement(18063..21734)
                     /gene="brxC"
                     /locus_tag="PW224_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HDL2908556.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(21759..22334)
                     /locus_tag="PW224_001870"
     CDS             complement(21759..22334)
                     /locus_tag="PW224_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HDL2908557.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(22334..22933)
                     /locus_tag="PW224_001871"
     CDS             complement(22334..22933)
                     /locus_tag="PW224_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HDL2908558.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         6993 a         4532 c         3344 g         8170 t
ORIGIN      
        1 tttctgggat agaaagaaca aatttacctg tatcatcaat agttgtttcg gacaaaacag
       61 cttgattttt tttcaatgaa accaaagctc ctgcttcgcc tgtacctgtg attgagcgag
      121 aagctgcatt gatcaatgtg gtacttaatt tagcaggctc aggaggaatc acatcgactg
      181 tcgttttggc agagtccagt tctgtcactt cttggccgcc tcttactttt ttagcagcaa
      241 ctatttgcac ttgatccccc gttgttagtc tttcttcgtt gggtacttga acctcaaaca
      301 aaccgccttg ttcgccttct ccccacggat tatatcctgt cccttcattt tcatctgttt
      361 gtccttgtgt gagagcagta atttctacag gtgatttcgt cgtatctgca taagtaatac
      421 gtaaagtcac ttctacttcg cctgtccaag catctcttgg ctttgcctcg acaccttcgg
      481 gaacaacgac atgcccatag atttttttat ctgcattcgt tggtactctt aaggattcaa
      541 tcatcggaag ctggttattg gcactgattc gattataatt tttcattccg gcacctgtct
      601 gagcaaattc atttgttaaa cctgcactag ggcttgatac aaaattacct aagtcggttc
      661 cattgatcga taagtctgtc atatcagaaa aataatccgg atctttttca aagttatcga
      721 acgttactcc tgcctttcgc cataaagcaa tttctgaatg agcgatcttc aaagaagaat
      781 cgccatttcc ttgaaatacc cagccaccta tgccattagt ctcgcttccg atccgtttgt
      841 tttggaaatc gaaataggta ggagattcta agtctacatt caacacatcc ccactgatga
      901 tgccagtctt atcttcccca gttttcccga ccatgactaa ataagttttt tcgccagcca
      961 taaagttcaa atcaatatct ccagttgtat ctacggtagc tccggaatct gcgcgtatat
     1021 cgatatttga tttttctcca gtaaccgtaa aatcagctga ccctggactt tctagaaaac
     1081 cagaagcgtc ataaaattgg atcgcttggt ttccttgatt gagtctgttc acataagatt
     1141 tgccatcccc tgcatttctt accttcaatg agcctccatt agttactgtt agtttttgcg
     1201 taccgttctc aaaacgaact gcgctagaac gaccactttg cttatcaata tcgatgactg
     1261 atccggctcc ttctacgatc aaagaaagtc ctttccctac aaaacgcatc ccattgtttc
     1321 caggttggtc gccatctcca gccattttga ccgagatttg tccaccactt gatacatgga
     1381 tttgactaga atctccttgc aggcgaaatc cgccagtagt cgctgtattg attgtgattt
     1441 tcccatcatt cgacacagcg actcttgatc ctgagcctct catttggata attccgcgat
     1501 tgttgtcgtt ggtaatattg atctttgtat ttaccgaaaa ttcacttcct gagccactga
     1561 ctttaaatgt gacattcccg ggtagcgtat cgacagcctc tccttcatcg ctgacgccat
     1621 ctgcccgatc atagtcaaga gagacctttg aaccactcaa gatatggagt cctggtgctg
     1681 tttttccact gcccgtctca tcacctgaat ctagaaaacc atagaatttt cctagatatg
     1741 gtccataaag ctgactacca ttgattacat ttagctgata gcttctacaa aaataagctt
     1801 tacccggagc ggcattcatc ccttctttca ctggttggac atccaataca ccggaaacac
     1861 catctaatgt gacagttgca cgaggggcgt aaatcaggcc taatttagaa ccatcatata
     1921 agtcaagtgt cccggtaaat tttatttctc ccgtaaaatg atatctgttt ccccaaaaat
     1981 cggacatata agcaatgata atggcatttc catcgatatt tggtatcaac ccacctggat
     2041 gagctgccgt tcctttaaaa ttctgaaact cgatcgactg aggtgtctca ttgatataga
     2101 tgtgatgtgt gccgaaatta tgagcaagtc cgccaaaatc gataacgata tctcgcttga
     2161 tctccaattc cctgtctaaa gtcaaaggct gagttaaacg aatcttagct actgattcgt
     2221 ctgcgagaag ctgcgtcagt tcttctgttg tggcagcttc gctgtaccca tccgctgaaa
     2281 caactctaac ctgtacctct tcactgctat cagacgtttg ttcagctact tctgaggtct
     2341 cacttgtctt attttcttct tgccttgtat tagaagtagg cgcctcactt tgggattctg
     2401 tagtttcaga agtaattgat tcttctgaaa aagtagtctt gtcaaaactt tgattctctg
     2461 attgcgttga ttcgcttgtt ttctctacat cggcaaaaac ggatgtcacc atcaagagag
     2521 ggaagacaaa caaaatcgac gcaatcagct tttttacata ttgattcatt ctccaccatc
     2581 ctctctgacg ttttgacaat tttattttaa cgttttaatt tatttgtaat tgtactttca
     2641 tatttaaaat atgtattttt atggcaaatt tttctgttag gtgaaaattt cttttttgta
     2701 caaaaaaagg ctgtgacaaa agtcttgtca cagtccctta ttccgaacaa ccgatgtcct
     2761 cctgacaact cttcggtcac aacctccatt ttaacaataa acctgtaaat gtgagggtaa
     2821 cactttgatt gtcacaggta atttatcccc ttcatctcca tcaacattcg tcgtcaattc
     2881 ttcctcatca gctaatgaca caactaattg ctcacaagta agataggcta gatttttcgt
     2941 agattcatct actcctttta ataggtctgg caatgtttta agcgtatcga tcaaagaaga
     3001 atcttttaaa taaaccaaat ggagcttacc atcatccact tttgcatttg gaagtaatgt
     3061 ttcaaaaccg cctactgagt tagttagtcc tatcagcaag atactgctct caatttcttc
     3121 cttctttcca tcaacttcta cgtgaaacga gtaggattgt gtggaagcta actgtttgat
     3181 tcctgacatg aaataagcta gttttccaaa tttcgtcttc ttctccgcat ctacatcatt
     3241 gattgcttca gggatagagc caattgcgac gacattcata aaatagtcgt catttatttt
     3301 tccaatatcc aatggcttga ctgattcaat cgaaaaatga ttgatcgctt cctcaggttc
     3361 tagcggtatc cctaatgctc gagccaagtc attcaccgta cccaatggga aaaatccgaa
     3421 atttggccga tgttcttgtt cagcgattcc actgattcct tcgttgactg ttccatctcc
     3481 gcccatgaca aacacactat gatacccttc cgtagccgct tcgcgagtaa agttttttgc
     3541 atcccctgct ttttttgtat gcaagacgac cacttcatca aacaccgatt ccaattttgc
     3601 tatcgctaat tgctcaaatt cctttgcttg ttcaccgcct gaactggggt ttaccactaa
     3661 taaaactttt ttcatcactt tctttccctt ctgattgatt tctttaatat aacgatagca
     3721 taaagaaaaa aattaggctg tgatgaagta ctgttcatga aacattcatt tattttttga
     3781 caagataaaa tacccatact atgaaactga ggaaaattaa aacaatgata taggaaataa
     3841 aagaaaatga taggcattgt atacctaata aagtaaaaat cttaggtggc agataaaaac
     3901 acaaaaatag caagagaatg taatagcgtt tcttccactt tttgttatca gaccttatag
     3961 cgatcagagg aataccaaat gcataactga aaaactcttg atgtactgtt aatatgtcac
     4021 gaccttgaat aataaatgca acaatccccg ctataattag tgtgcctaat accaaaacgg
     4081 aaaattgatg aatatatttt atatacaaat gatccattgt acttcctgcc tttttatttg
     4141 tgagaaaaac aaatccgtat acgtgaataa cttctagaaa taaaagaaat ttgcaaaacc
     4201 actccattta tctacttcta cgtgcatttt ttaatatcgt tttctttatt taagattata
     4261 cagaaaaata tctagggtgg ctggattcta agtaaaaaaa atttttaaaa taaaaagaac
     4321 taaatcaagt aataattaaa ctactgttga tttagttcta ataaaaagat ttagatgaac
     4381 agttttattg atttaaatag ttcatatttt ttatttatac cgcttattta ctacttgctg
     4441 tactagaatc tgaagtgaat gtactcaaaa cattttcaaa gtctttgtct ttgattttta
     4501 cattagcgtc tttcaatact tcgccaatta ctttattctg gaaggtactg tcacttaatt
     4561 ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt atccatgtcg ttgcctttgt
     4621 tttggttttt caccatttta accacataat attccgtagt ataagttgaa gcattggtag
     4681 aagtgatcac atcagaaact tgaccatctt tcaacttaaa ggctgcttct ttgacttctg
     4741 ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc gccatcttct tttgtcgtag
     4801 tatcagttga cttgtctttg gctaatttag agaaatcgtc tcccttatca gcagctttct
     4861 tcacatcttt tgcatcatct tcacttgcta cttggataat ttgagcttcc acttctggat
     4921 ggaaagattc ccaagccgtt tttaaatctt catcggtcaa tttgatatgt ttctttagac
     4981 cttcttgaaa ggctagactt tgtttgatac tgtctttgaa tgtttgttct gtgtaacctg
     5041 cagatttcaa ttgtgaatca aatgaatcac ccaattgttt ctttgtttga tcatattggg
     5101 aatcgatttc tttggttgat acgtcatctc catatttatc ttcaaatact tgataaacga
     5161 tcaactgttg cacagcggaa tctgctcctt gcattttttt caagctatcc attacttctt
     5221 gttcgctgat tgtaccgcct ttataactaa cggctgtttt ttcaccggca ttcgtacagc
     5281 ctgacaataa gccagtgcct agtacgatgg caagtgctaa aaatcttttt ttcatttatc
     5341 aattctccta cttttcgttt tttgcgaatt gctaaaaaca aaggtgttca agatgaaaat
     5401 catcaagaac acctttacta atctgctcga tacgtcctga atagatataa ggctacacaa
     5461 tccagaaagt tcgctcagca gaataaatgt caagtagcac atcacacctt aatatactac
     5521 ttcttttgtt accgtttttc aagtctatct tgcgttccgt atttaacgta actaattctt
     5581 agaacaaatg ttatcgattt tgtttttctt cttccgctgt tgggatattc aaatcagaca
     5641 cttttatgtt cgattttcca gacatttttt tgttgttttt attagaaaaa gtttgttttt
     5701 tcatttgttt tctaaagcat gatcttcttg tttcctattt tggtcagata tggatcatgc
     5761 gtgaccacga taactaacgt accttgggaa gcctgttgcg ataagatttc aacgacttgc
     5821 tccttatttc gtttatctaa tgatccagtt ggttcatcgg ctaaaatgat tttgctattc
     5881 ttcaataaga ctctcgcaca ggcgattctt tgcttctcac caccagataa tgtgtagata
     5941 ggcgtactta gcttcttgtt tatatttact tttttcaatg cctcatactt tgctgttcgc
     6001 ttatctgaaa catttaaatt cgaaaacttc aatccaatat ctaaattaaa atcgattgtt
     6061 tcatattcaa tcaacccaaa atcttgaaaa agaaaagaga gggcatttct ttttatcaag
     6121 cgcttctctc ttccagttaa atgttttgtt tcttgattaa ataaataaaa ttgccctgaa
     6181 ctgaattgat cattcaatcc taatatattc aataaagtac ttttccctgc tccgctctct
     6241 ccaactacag tcacaaaatc cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt
     6301 ttgtttcgaa tgattttttt cgcacctttt atcactatca acggctccaa ttgttaacct
     6361 ccattcatca gcaataactg acttttcttt tctgtagaca aaataaaaac aactgaaaga
     6421 ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc tctttctata
     6481 agaaaaagga agattacact tgtcgttccc catttgatga ggttcatcat gaagaaatct
     6541 ttatacgttt caaagagcga ataaccactt gaacgaaaga tagcgatttt ccgacaattg
     6601 tagacaaaat atatgcataa catgacatag ctcaacaaga agaaagaaaa gaaagtgagt
     6661 aaaaatatct gtatcaagta atgaaatgat ctgcttaaca ctttgacagc tatctcttca
     6721 gtctgagtat acggaaatga taactgcaag ttatcacgta actgactttc ttcaatcagc
     6781 tcaaataacc tttctttcga caaagagcct atcttgatct ttaacggtgg atagatttct
     6841 ccgctgagaa tatttctttc ccaatacgta ctgtttttca atgtcagtat ttcaacaacc
     6901 ggataatgtt cgatccaggg gatagaagga ataaatgtat aaatgggttg attgtttttt
     6961 ataaatagaa atttcggttc tttttcactg atttcttgat aatattctgt tatttgcgcc
     7021 aggtcatttt ttaaatctgt atgtctatat cgttcaggga tcaatataac agggtgcgtt
     7081 tctttttcgg ttatttggat caaccttcca gagacatcaa tcaaaggaaa tttcttaaga
     7141 taattcagat ttatttttat ccctctgcca aactgttcat tttcttcatt caagtaatct
     7201 gatacattga ctaatatact tccgttttta ttcaaagttt catataattc ctgatcggct
     7261 gctgatgcaa aacgattgga ataaataaaa tccacgtggt ttttcccgat ggttactgga
     7321 taaaatacat gatagtcctc accagcgatt tcttttggtg tttcatggga aacagctaac
     7381 tgtgaagaag aaaaaacgat agttgctaat tcgacattgg caacggtcaa aagaagagaa
     7441 aacaacaatc caaatgtaaa aagaatgtat tggatatagt tgaacgcttc tcttaacgaa
     7501 gcgatttttt caactaataa aagagaaatc aaagagatac cctatattcc gctgaacaag
     7561 aaaacgtaca tctggaacaa ataaagaaat tgctccttat aatacataaa aataaaacca
     7621 ccaatgattc ctgcaattat gaatgtaata atgattttct ttttccccaa taaacaccac
     7681 aatacttgat gtaatgaaaa gccattattc aacaataaac tgatttcttt attcatcaat
     7741 aagaaaaagg atgataacaa aaggaataaa agaatcaatc caatagttaa atacactcga
     7801 taattatata cgtctgtact tgaaataacc gaatacgaat agctattttc aaagtcttca
     7861 aaaatattag tggcataatc agtgtgaaat gcttgattga atcgttccac aaatagtaaa
     7921 atgaaccgtt cataggcttc tttttctctc gtcatcaaga aaaattcacc ttcaaaatct
     7981 tttattttga tcgattcttc aatggacgtt acgcgaaagg aggtattaga atagggtacc
     8041 ttctcgtgag aagatttgac atccccttga taatacaatg tcatttcaag ctttggctgt
     8101 atcttgaagt tccaagatgt atctacttca ttggattcat ttactgtttt tttcatataa
     8161 gaaagctcag tttctgatga gacattttca aataccatga ttagctgctc atattcttct
     8221 tgcccaataa aatgttgtgg caatcgaatc ggagtataag tatctgtttg atggaaaaat
     8281 tcatttgtcc gatagagtac ctgtttattt ataataacca aggcaaaacc actcatgata
     8341 ccggtcacta tgattgtgag taaaactgtg aacttttttt tgattatcac cactctccct
     8401 tacttaaatg attctttgat aaaatcatac cgtttttcat caaaaaaacg tatctatcaa
     8461 atgtataggt catgtagaat aaatctatat actgtagaat cgaaaggaga agaagatgaa
     8521 catcggagaa aaaatacgaa cttaccgcag aaattgtaag cttacgcaaa aagaattagg
     8581 cgaaaaaatc ggtgtttccg ataaaacaat ctctagttgg gaaaattctc gtacaatgcc
     8641 agaccttgaa atgctgtcac ttttacatca gactttaggt ctgccgattg attttccttc
     8701 tattgaatca gctaccgaat caatagaacc aacattgccg gatgaacctc ttgttatcaa
     8761 gaaatcttat tcatcaaaac aacccttctc actcaaaaag acactatctt tctttctgct
     8821 cgtcttttgt ttggtctatc tttttcttat caattatcaa tataaaaatg aaacggttga
     8881 tcgattcaat ccttttttga aaccacaaat cggctatacc acactccctg taggatacgt
     8941 ggaaaattcc atcgaacatt gggtatgtaa tgatatcttt gttcatggta catggctgtc
     9001 ttttcacgct ggagaggtag aaaaaggtaa gaatcatgct gtaatcaaac acaaaggaaa
     9061 acacgtcatg aacgttcgcc tgctcacttg ggaagccatt ccctcagatt accaagaaat
     9121 cctcatccaa gactatgacg atgccttaaa aagaagccct caggagccag ataggcatta
     9181 agtaggagat gaacgtagag atagaaaaaa tcaatgcaca tgatcttatc aaaaaaaaac
     9241 aggggtacaa attaattgaa aaagctttga gatgaaagtt cctaaataaa aaagttcaga
     9301 atgataggta aaaaaatgac taactattct agtattaggc tatttgtatt attcttctgt
     9361 tatttttata tcaaaaaatc cagaaataac taaatatagg tatttctggc ttatagtata
     9421 atatttacgc cacagccccc aatgattctt tttggaatca tgatcaagga cggacataca
     9481 caaaaatatt ttgcccattc gatccgaata gattaacgac taatctagta agtaatatag
     9541 attggtacgt agtcaaacca gcaaatggaa aagttgtcta ttccacccca agggctttaa
     9601 agacagcatc ttccggcgtt tgataaaaaa tcaaattaaa ggtacccatt aattctgggg
     9661 gtacagttcc aatatcagca gcgcttatca taggtacaag tacttttttc gctccggcat
     9721 cttttgctac ttgtaaacag ttagctaaat tatctacctt aatcatagaa ccaccaattg
     9781 taatatcccc taaaataact aaacttggta atacaggttt atttaaggaa actgagcata
     9841 aagctattat agttgataac gataatgttt ttgtcatacc aatgccattc atatcttgaa
     9901 catgaacgac aaaatctttt gtcgtcaaac taatagactg actaacactt ccataattag
     9961 ctcttaaata tcggtatgcc gaatccatcg cttctcttgc tccagcatct gaattgatcc
    10021 cagtacgttc aaactttcca tttcccgaag tcatttgcgt ttccaattta taggtaccta
    10081 attgcccagt tttgccataa gataccgtgt acaaatgccc tggcttccct aacccttctg
    10141 gaatcaaagt tcctccacct tgttcaggaa cagaaacata ctcttctcgc tcttcttcta
    10201 aatcaatgta tgaaaaattg acatcataaa attccattcc accaattttc tttaattgtt
    10261 cttttacacg acgacgcatt tcaagagcaa atcttagtat ttcttcgaca tcactttttt
    10321 caaatctacc atcaggatac aacagtttca tcatcccaga tacaatccga cgaactgcaa
    10381 tcgtatcccg ctgatttaat tgagaaccaa gtttgaaata tttatcaaaa ctatcgccaa
    10441 aactaacttt tcgaagctca cgcatgactt cagaaaaata atctgtaatg aagccatagt
    10501 catttgtaaa aaaatctggt cgatatttag gaatttccca acctggtaaa taacaatgca
    10561 tccgatccaa aaaggctgaa tccgtcccca taacctccgg aaatggctca aataaattcg
    10621 aagttttcaa tattacatca acgctttgat taatatttcc tacaaaagcc attgatgctg
    10681 aagcattctt ttcttctttg cctctagcaa aagatcctga agccatgtaa tctttcatga
    10741 tttgaacgcc atcattatcc ttaaatttaa tcccagcaac ttcgtcaaaa gcaacacagt
    10801 cccacatacc tacaagtccc attgtacgac tagccatatt atagaaaagg tttgctactg
    10861 ttgtttgtcc acctgaaaca aggattgagt ttggtgaaat ttctttatat acatgcgatt
    10921 tacctgtacc acgtggtcct agttcacaga gattaaaatt attttcaatt aatggaataa
    10981 gtcttgctaa atgaagccat tttacccttt tattaaattg tgtaggttcc ataccaatgg
    11041 accgtagaag taagtcaatc cattcatttg tagtaaactc cttgcgagcc atcttgtaag
    11101 cctcaatgtc taaaccaggc atttggattg gatcaacctt ttttgcaata aaagggctat
    11161 tccaacgatc attctcatca taccaatatt ccatcgaaac aatacaccag atccccccac
    11221 ctaataatcg atcaaatttt ttcggatact cttcactaat ttgaacattt tttaatccca
    11281 ggttagagaa ttctgcttca taacaatctt tcttttcatt taaatgtaca gttactttat
    11341 caataactgt ataaccaccc aattcacgaa tttttgcttt tactttttct gcttcatcag
    11401 gacgcacata gtttcgagca agaatatttt taacattttc cacacctttt tcaaccaatg
    11461 tttcatccaa tgacgaagca tacataccaa gtaaatattc caatacataa acaggtacat
    11521 tcgcaccttc tttaattttc tttgttaaat cttttctgac aatctttccg ggaaagaatt
    11581 cagataactt taaaatttcc tgttgttcca ctagaattcc tccatgtgtt cagtcattat
    11641 aatagcaatt aaagtatatc atacttaacc agtaggcttg ataatacaaa agaaaaagta
    11701 tccacaaaaa attgtggata cctcgttttt atatatcaaa atcaaatgat tctgcaaaag
    11761 gattatctat aatgaatttt attcgctcgg tcaaatcatt tttttgattt tttatcacta
    11821 aataataagt atcttcaact gtatatcgac gatttacaaa ttcaaaaaca aatttagtaa
    11881 accgctgatt tgcatcttct gtttttgaat ccgcaacata gatttcttca tttgagatac
    11941 gttcaccata ttcatcttca aaataaagcc agaaagatgc ggggtcataa acatcactaa
    12001 ttgcattttt ttgatagaat tctaacgtgg ttgataatcc tgtaattctg cggttgctag
    12061 tcattaattc aattgctact ggttcctttg cattgctacc tttggctacc gtaatttcta
    12121 gaacagggac tagcatttct tgaatagatg accctccatg aacgtagctt tgtcccccgc
    12181 cagctttctt aaaaatactt gaagtaatcg ggacacaaat gtttctagtg tcatcatttc
    12241 ctaaagaatc tcccaaagag taatttttcg taccaattat ttcgtattct tcttcactaa
    12301 gaataaaacg acgttctaca cgatcttttt catttctaga tggattttca atcttatcta
    12361 cttcttcaac gtggcttctt ttataaataa atccatgatc agccgtaact ataaaacgat
    12421 agatactgcc atcattatgc aagcgattaa taagtcggga aatttcgctt atcgctaaat
    12481 cacaggcctc aaaaactcga ttttcattcg agtgatcacc tgttctatcc acttgatcgt
    12541 gataaatata aatgactgat ttgccattaa atacttctcg tacctcagcc ttattcatct
    12601 taaccacatc atcataattt aaagctacag catttgcatc tcgttgctgg agaatcgcat
    12661 ttcgattgga taaaccattc gtttttttcc catcaatcag tacttctgtt ccttcattgt
    12721 aagttagttg attgtttggt aataaggcgg ctttaccgaa ttcagtaact gaaggtaata
    12781 cagaaagaaa gctagccatg tttgttgaat acttcttttc tctaactaac ttacgttcta
    12841 aattcatccc taactcatat cgaaaagcat ctgaaataat aactacagtt ttaacagagc
    12901 gtttcattac agcttgatta taaaattttc gaatggatgg acgattctta aattcaaaag
    12961 tttgattcca aatttgtcct gattgatcaa gaaacttcga ataatttgct tcaactaatc
    13021 gatacaattg gccatatttt tgcttttctt ctatatgatc actattccaa ataaatttcc
    13081 gataatgcgt atcaacccaa tacatttcct tctcatattg agttagcaaa ttatccatgt
    13141 tcatcggcaa gcacaatctg tttttatcca caagataata agcatgtttc aataattgat
    13201 actcataaat ataatccgat gcgaaatgca ttttttcgcg ctgctttaaa ctatcaaaaa
    13261 ttgtttgtcc tgcaattttc gttgtagtgt cagaagcctt taatcgttct gcataaaaca
    13321 ttaatatttt tgcatgaatc tcagaaaata cgtctgcttc aattagatca tcaatttcta
    13381 tactgtctaa taatccatca ccatcaatag cctgatatac ttttttagat agctgttcaa
    13441 agctttccat gtaacgtgta tcattcataa actgatccat gaaagtaata atgtttgctt
    13501 tcaaatgtag tatttcaaat cctttgaact tcttcggcat ctcttgatca atttgataat
    13561 aaaaatagtt caaataaaga cacatcgtca gcttttccaa tgttggctgg ctatccgtaa
    13621 agccaaattt catttgaata aattcccaaa atttagctgt taactgatac ttatcaagtt
    13681 cccttaaagg attctcccta ctttctgtaa agctttttag aactacacgc agtaaatcat
    13741 ttaatcgaat acttttcgat ttcaacaaaa cagccattat cattaattca atcgtttctg
    13801 atttattaat tgtcatatgc gtactaaatt caccaaaact tctcatgcga tcctttgcat
    13861 taaagaaatt acgatactgt ttaactgtct gatgaaatct aagtggaata gccaattcat
    13921 tcatgataat cgctaagcgg tctgcactaa attcctctga ataattcaat acactcaaca
    13981 aatgattatc ttcatcttta tcatcccaac gaggaaatgg cgcatagatt aaatagttag
    14041 tttcgggatc ttcatattcc aataaacgct tagttttaaa ttgtgcgtcc ttttctaatc
    14101 gatagatttt tgcattttga atgaccaatg agtcaatttc ttcaagaaaa tcctgatttg
    14161 aatcaaacca aaaaatgatt ttcctacatc cataattagg aaaacgttga tttaattttt
    14221 cttcaatttg ttgcgtattt atatctgtca tcaatctcac ctaatcgttc tctttctata
    14281 ccccttatat tgacataatt tggataattt cgcacgcaat ttaagaataa aaaagaaagt
    14341 tgttagaaaa caactttctt agataactat ttttttggtt ctttgacccc ttttgcaaaa
    14401 atttggaatt tctcgccggt tttaggatca cgctgcaact tatcataatt gacaattaca
    14461 ccatcatcta agtctaattc aattcgtttt aatgctaagt gacccaactt ctcgtcatac
    14521 tcacgaactt cttttaattg cttctgaatc tttacaattt cttttgtcat tgcatttctt
    14581 tcttttgggt tatcacttgt tgaaatttgt tgctgacgca attcgattcg attgtcatac
    14641 gctttttgta gttcgtgtag gtattcagta cgtacttttc ctaattgatc tggtgtatag
    14701 cgatgcaagt acattaacgc tttaaagcca ttttgttttc ctgagtccaa ttgccaataa
    14761 attggtcgat tggagtatgt ttttaaatgg tctttgtaaa aatcttgtac gaagtaacga
    14821 cgaataattt ctcgacttgt ccctttgcca cctaaagtgt cagcgataaa ctggagattt
    14881 tcttcaaggg tttctttacc aaaagtaatc tcaacgaaat caatgaaacg attgacaatg
    14941 tcaatttggc tatccttgaa atactcatca tcggtgatga gtaaaacatt atcagtatca
    15001 ggtttaaatt tttgatagcg ggaaggatcc cattcgccgc cagcgaagat gagaccatct
    15061 tcgtctaggg agtaacgacc gaagatacaa cctattgaat aagaaataaa atccttaatt
    15121 tccctcgcct tatcaggtaa ccttgcagag atatattcat cttgaacctc ttttgttaat
    15181 tctttttcaa gagaataatg attaatgaat atgtcgttca gttcctcttc attcgatttt
    15241 agagtattaa attgattaac caactgccta cttagacagt taaatgcctc tgagagatct
    15301 tttttaaaca tataacttaa caaaatgttt cttctaaact cgtatgacgt ttcaagagca
    15361 ttatagtctt ttctagctat atcgatattt tcatccactt tttctcttat tttttttaat
    15421 tttctctcag atgtaacttt aagaaaaggt agttttccca ttggtccttc atggtaatcc
    15481 aaggtgggag aaataaaagt taaaaagtga ttaactaatt tagagtttac atagccaaat
    15541 atttcttcaa agtcagttat atcctttgag aacagaacag atcctttagt ttcaaaaatt
    15601 gcctgattct gcaatctaaa agatacttgt ccagatgaaa gtgtcgacca agtaattccc
    15661 tttttaaaat agtaattaag attttgtggt ctagatttta acttcccatt ttcattaaca
    15721 taatttttta tttcatctcc atcattctcc cagttaacca cgtattcatt gtttccatac
    15781 cattttctaa aacgaccacc tttagttgta ggaaaccatt tttttctaga ctttttagcg
    15841 tcctctaatg ttttagcatc aaactttatt ttgttaaatt ctacttcaaa ccaataccgc
    15901 aaaaatttat tgttatttcc ggttgccaac ccttgttttg catctgcaac atcgttagtt
    15961 aattttcctt gagcaaaatc agtgattacg ttatcgcttg cccagtaagc aatcggcatt
    16021 cctgggattt ttttgaagtt gtcttgatta gtactataaa aataatcgca ttcaccatgc
    16081 tgatgaattg cttctaacgt tttaattttt tgaacttcca ttccaccttt aaaatctgat
    16141 aattttatat agcttccatc ttgttcattt gaatttttta tcgtaaacgt acaaattgga
    16201 acggttgctt cttcaaaagc tgaatactcc atttgaatca aactagaaat gtttctttca
    16261 ttaattaaaa atgagcgtaa cttttcatag ctagaaatga acatccaaac aaatggcgtc
    16321 ataaaggaag catatccatt atcaatcagc atttggctat tcaatttaat aaacacagaa
    16381 aatagatctt ttttgacatc agaatagtta tcatttacat atttttttaa tttcttatcc
    16441 attttattaa gataaggcgg attagtgact accgctacat atttatcaac tagcatttca
    16501 gccacgtcaa gaatgtgcaa gtatttttct tgtatttcct tcatcccata aagaatatcc
    16561 atattcgtaa attcgttgaa ttttgatata tactctctta cttttcctaa gtattcttta
    16621 gttattgcct gattattttt atcgactaac ttaatgattg atccaagttc tttcccattt
    16681 tcaaattgat caaaaattaa ctctttgtat tcattccagc gttcactata cttatcattg
    16741 ttcaattcac taatacgctc ttgatactcg tctgtcactt taggagaatc aataaactct
    16801 cttaaattcg gcgatagact ttttttcaaa aaaagacgat cgtattgacg ccctttcatc
    16861 ataattgcaa aataagacaa ttgatacgct cttttatcga tttcaagacc atataaattg
    16921 tattcaacga ttgcacgtgc agcatcacgc tcagaatatc ctaacgtaag ataaatttcc
    16981 atcaatacat cgaaggcgta aactaaaata tgcccagacc ccattgcgtt atcgataatt
    17041 tttagttctt ctggttttaa attacttaca gatgttgctt gttcaaaagc atatttatct
    17101 ggaattaagt atttcaactt atttcttaat tcactattgg gataatgatc taaccaatac
    17161 tttcctaaac tattgtcaac catataacga acgacccaat ctgtggtaaa tagttgagtt
    17221 gctgccggaa tatcttgttt tttgacaatc cctttataaa tattcaccac ttgattatgt
    17281 ggctcactat tatagtactg atacaaccac ccaataatct ctacctgtcc accagcttga
    17341 acatagaaat catcctctgg aatacttaaa agatcagcga ttacaccatc ttttttaata
    17401 taagaagctg taaacaaaag ttctgtataa tcttctactt cttcaaatag ctcaggtaag
    17461 taatcattca attgattaca gatttttata aaaatcaatt gatacagctt gtccattgct
    17521 tctgctgaat tatctgtttt ccaagttctg acttgttgct gttcattact agataattct
    17581 tcaaagatat cggcatcata aatatctgta ataatatctg gctcaatttt cccacttatt
    17641 tcactagata atacacgata cgtgtcaggt aaataatcat ttacctccat aaaccgaatg
    17701 gcaatcaatc ggttaaacca cgtataagca acctcttcga ttaactcttg aaaagcttct
    17761 gaataagaag tgacagaagc acgttgtttt agttctgcaa tcaatttgtt tctttgcgct
    17821 atttcttttc cttgaattct tattcctgat ttttcgattt cttgtaaatt gctatcaatt
    17881 ttcttcacat cttcaatttt tgattcatca attcctaaac gttttgcttt agtcattact
    17941 tcatcaatta attttctacg ggcattgacc gcgaaattct ttatcgcttt cttatccatt
    18001 tttctttccc ttctatatgt aacctttgaa aagacaggca cctttttatg cctgtctttt
    18061 cactagaatt gaatcgtgat ttctgtattt tcttgaagtt gttccttcag tttttgatta
    18121 atttcagctg ttaactcatc aatatctgct gctgacttaa tagttttaac atgaacgtta
    18181 agtgttctca ttggtactgt tttctgttga atagtaggtt ttactatcgc tatttcttca
    18241 gcttttgctg cagagtctat ttcatctttt tcagtttcac gaatcaagtt tgctgcttca
    18301 atttgtttac gtgcttgttc ttcttgacgt ctgcggtttt cttcattatc gaaacgttga
    18361 ttaaagatca aatactgttc tgctacgttt aatgataaga aaacaagatc atctatagta
    18421 ttcgcttgtt caatttttgc tttcagttct tgaaattctt ggtcaactat tggtacaaat
    18481 tgatccttaa aatcttttcc ttctaataat ttaaatgttg cttgtttttc ttccgttaat
    18541 ttatttaata tttcctgact ttccaactct aacagttgat tatattgttc aataaattct
    18601 tgaatcaact catttagttt catcatatct gaataaggat cagccttttt caaaatggtt
    18661 ttcatctcat gaatcaattg atcaatttct tggctagaag tttgacgccg acttttttcg
    18721 attaatctaa ttttatcttg tgcattctcc cagatttttt tctgagtact attttgcttg
    18781 tggaaatttt taattttatc atatgtttca gcccagtcga ataattcatc gcgatatttt
    18841 ttgattgtat aaaaaaagtc ttcaacatct gacgtttgaa caattacttc aagatatttt
    18901 tttatttgct catattcata agcactaggg taggtataag tgttttgtaa atcaaaataa
    18961 gagcgtaaat tgtctaattg accttttaaa tatttcttga aattttccat tgtatgttca
    19021 ttgttatttt catcaacaat attacgactt tgaaacagtt ctctaccgat ttcgtcaacc
    19081 aatttttttg cttttggcga aatcaccgcg cttggttcaa tttttatctt ttcactttct
    19141 cgtcgttttt gaataatcat tacaatacta tttggatcat ccatccgtga aatttgtaca
    19201 ttgttataaa gtaaatcaat tttaccagac atgaaaagtt tagcgatgca ccattccaca
    19261 tcaagttctg tgaatccata cggttgtttt gtttcaaaac gatcacgaat tgttttcatc
    19321 gtaatcgtta ggtgatttct cttttgatca ttgataaaat tgaaaatttc ctgaactgct
    19381 tgctcatttg atggatcttc aataatttca tttttgcttt ctttcactaa atataagata
    19441 tcttcttttt ctttaggcgc atcaatttgg tttaatttat ggtatacttc ttcagccatt
    19501 ttctgaagtg ctgattggaa ttttgcttta aagtcggagc ctgtcagttg aatttctgca
    19561 ccgttataga aaaattttgc agatttcaac agttcattta attgatcgtt gatacttgca
    19621 cttagttcct gtaattcacc agatttttcc gaacgaatcg cgtcaaagtt ctttactaca
    19681 ctcgaataag tcgttgttaa ataaagattc attctagcat acattgctat atcatcttta
    19741 aattctttat ctgatggcaa ttctacgaat aataagttac gggaactctt aagcgcaagt
    19801 tctgcctctt gacctgctaa atcagagtat ggtgaaataa aattaactgt catttcagca
    19861 tttccagctt gcttgtatgg acgttgatct aacacttgtg cataagtaaa cgtataccgg
    19921 tttttaaatt tatttccacc tgacatgcct aatttttcat aggcaaaact atttcggtct
    19981 agaacgccgt caaaaattcg tttagatagt tctacgatca aatcactatc tgtaatattt
    20041 tgatttttaa tctcacgagt aatttcctgt tcttcatcag tttggaaaat ataactatct
    20101 aagttcttcc gcacaaagtt ttccttaatt aaaaggtcca atgcttcttc tactcgtttt
    20161 ttagtttcca ggcggtcttc gttgatattt cttatcatca agctcgtaat attttcaaca
    20221 tttggtttga tggcttgatc aacatattta atcatgaata aaattcgtaa gacatttacg
    20281 ttaaagttat ctatatcagt tggtctttga atatgagaat tatttgtagc ttgtgtaatc
    20341 actcggctaa ttgctgtatc aatccaacgt tctaacgagt aataaaactg atctaaagaa
    20401 accaaactgg ttgtatcatt ttccttattt cgttcagcgg cttctttaaa cattgctagt
    20461 aatgaacgtt caccagacga taagtttgat ccttgatagc tgtgattacg aatctcggtt
    20521 agaatttgcc ccaataaatt gaattgatag ggaataaatg gataaacttc tgcgtaattt
    20581 tcagcatctt ggaagagctt taattcagga ctatcaacaa agagtaattt atttttcaat
    20641 tctgtttgaa cagtttcaaa ttgtaaggct aaagaatcat tggcaaactc ttttttcttc
    20701 aaaatacgtt ctttaattac ttcatcggca tttgctgaag ataaactcac agttgtggca
    20761 aaacgatcct tgatcttaga aaaatcatta tcagaattgt tatcaaagtt tgttacgtga
    20821 tcaatcgctt cctgacttgt cactaatacc caagccttac cttcacaaat tcgacccaaa
    20881 tcttccacaa cggtttgtaa ctgcaaagtc aactcacgat taccgccgat atattgtccg
    20941 acctcatcga ctagaaaggc aatatgatgg tctgcttctt tggaatcaag atagctttta
    21001 actagatttg caaagtcatc aatgcttatt aaataatttc ctgatgcttt ttctaaaaaa
    21061 ttatcaatag cggcttcact cataaaatcg attttaagtg ccgcttcttt gatatcatct
    21121 tggatccaat caaactcatg gcgctcttct tgccattttg ccccattaat ctcttcaaaa
    21181 gcagctttga atgcatcaaa cttttcttct ttaattaacc gacgttcaaa atctgctaga
    21241 tgtggaatcg tgcttaatcc ttgcatttcg ttaaatacct ttaaaaaaac attcacgatt
    21301 ccgtttttgt cttttttacc acctgcatca ctttttgaat caatattaaa cagaataaca
    21361 tctgtattta tttgtgagca ccgttccatc gttgccaaag tcagtggatc tttgattttc
    21421 ccatcttctt tgaagtaatc aatggctttc ttgtcattta tttctcgatt tgcgagcaag
    21481 taagaaataa tttttaagaa atgagattta cctgaaccaa agaacccctg aatccaaaca
    21541 cccattttat ctgttggtgt attgatactc cgttggtagg catcaaaaat tgttttaaag
    21601 tgtttttgta gttctttcgt aaccacgtat tcttctaatt cttgttgaac attttcatcc
    21661 tttgcttgac cgaccttaat aactccttga atatttcggt caattggttt tgcaaaaata
    21721 tctttaatta gcatttaatc tcctaccttc catcccaact aaattctaaa tgctcgataa
    21781 taattatcat ctttattggt attaagaatt gtcaaactta attcatcata ttcccctgga
    21841 tacatcatca caactggtac gcgatcaaat acctgatgca ttttatttaa aatagagtgc
    21901 gctctaatta atggatataa ttcgccaatt cctgtcagga acacaactga tttttctggt
    21961 gtatgggcat ccacatattc aaaaaataca ttttcttcct caggtaaatc cattttcaat
    22021 aaacgaccaa tgctttccac taatttttgt aaaccacgtc gttcttccat cttaatcgct
    22081 ttttcagtga aaccgcgttt ttctagatac tctaacataa tcacatagag attataaact
    22141 ttcacttcat aattatcacc aacagatgtt ccagatatat cattaatata atctcttaca
    22201 attaactcgt ctttcggatc gtagtcaaag atataatagc ctacttcatt acctaaacca
    22261 cgattcaata aaaaatcttc tttttgaatt acttcattca acttttccaa atgctcttga
    22321 atcgataatg acattataaa attcctccca gtgctagtac aatatcttta cgattcttgt
    22381 caactaacca ttgaatcaat cgtttatcta ataatatcgg agtcattcgt ccatattctt
    22441 tttcccctct gactaatcct gtttcaatta gattcgtttg ataagcggca ataagccgtt
    22501 taatcgtata atctttccat tttgaaatat cttctgaact tctccgcttc tctgtaaaaa
    22561 aagaacggta atcaactggt gttaattgca aatttccttt acgtagattt tcagcatata
    22621 cctcagtcat aaattcatta aaaagatcat caatcataag gatggaaatc aaattcacta
    22681 acttttgatt atctatattt aactgattga aaagttgcaa tccagcttca tccagttgac
    22741 taactctcct tttcacgttc tgatagattt ctttttgacg gttactgctc actgcaaaaa
    22801 aaatattttc atgttcattt ttctctttaa tttctatatc tgtatatcct tctagtcgta
    22861 attgaatata taacttgaac tctgaaaacc aaaagcgttt agaaactaga ccagcagaat
    22921 attttttatt caatagattg cctcctaatc atctataatt caataagtaa acaagtgttt
    22981 tttgctcatc gttagcattc attttttagt atctactata caatacatat ttattatag
//
LOCUS       DANKMN010000052        22464 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000052 DANKMN010000000
VERSION     DANKMN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            144..542
                     /locus_tag="PW224_001872"
     CDS             144..542
                     /locus_tag="PW224_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2908559.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1220
                     /locus_tag="PW224_001873"
                     /pseudo
     CDS             526..1220
                     /locus_tag="PW224_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1814..2701)
                     /locus_tag="PW224_001874"
     CDS             complement(1814..2701)
                     /locus_tag="PW224_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2908560.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2886..3725
                     /locus_tag="PW224_001875"
     CDS             2886..3725
                     /locus_tag="PW224_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2908561.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3921..4895
                     /gene="sgrA"
                     /locus_tag="PW224_001876"
     CDS             3921..4895
                     /gene="sgrA"
                     /locus_tag="PW224_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL2908562.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5193..5549
                     /locus_tag="PW224_001877"
     CDS             5193..5549
                     /locus_tag="PW224_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908563.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5552..6709
                     /locus_tag="PW224_001878"
     CDS             5552..6709
                     /locus_tag="PW224_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908564.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6735..7175)
                     /locus_tag="PW224_001879"
     CDS             complement(6735..7175)
                     /locus_tag="PW224_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2908565.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7335..7841
                     /locus_tag="PW224_001880"
     CDS             7335..7841
                     /locus_tag="PW224_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2908566.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8009..9922
                     /locus_tag="PW224_001881"
     CDS             8009..9922
                     /locus_tag="PW224_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2908567.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9927..10928
                     /locus_tag="PW224_001882"
     CDS             9927..10928
                     /locus_tag="PW224_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2908568.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11130..11336)
                     /locus_tag="PW224_001883"
     CDS             complement(11130..11336)
                     /locus_tag="PW224_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL2908569.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11982..12830)
                     /locus_tag="PW224_001884"
     CDS             complement(11982..12830)
                     /locus_tag="PW224_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2908570.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12829..13896
                     /gene="gnd"
                     /locus_tag="PW224_001885"
     CDS             12829..13896
                     /gene="gnd"
                     /locus_tag="PW224_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL2908571.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13898..14899
                     /locus_tag="PW224_001886"
     CDS             13898..14899
                     /locus_tag="PW224_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL2908572.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14896..15180
                     /locus_tag="PW224_001887"
     CDS             14896..15180
                     /locus_tag="PW224_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL2908573.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15213..16010
                     /locus_tag="PW224_001888"
     CDS             15213..16010
                     /locus_tag="PW224_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2908574.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16024..16857
                     /locus_tag="PW224_001889"
     CDS             16024..16857
                     /locus_tag="PW224_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2908575.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16875..17732
                     /locus_tag="PW224_001890"
     CDS             16875..17732
                     /locus_tag="PW224_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2908576.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17754..18263
                     /locus_tag="PW224_001891"
     CDS             17754..18263
                     /locus_tag="PW224_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2908577.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18300..18701
                     /locus_tag="PW224_001892"
     CDS             18300..18701
                     /locus_tag="PW224_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908578.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18813..19166
                     /locus_tag="PW224_001893"
     CDS             18813..19166
                     /locus_tag="PW224_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908579.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19200..19643
                     /locus_tag="PW224_001894"
     CDS             19200..19643
                     /locus_tag="PW224_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2908580.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19660..20745
                     /gene="uxuA"
                     /locus_tag="PW224_001895"
     CDS             19660..20745
                     /gene="uxuA"
                     /locus_tag="PW224_001895"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2908581.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20738..21451
                     /locus_tag="PW224_001896"
     CDS             20738..21451
                     /locus_tag="PW224_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2908582.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21658..21807)
                     /locus_tag="PW224_001897"
     CDS             complement(21658..21807)
                     /locus_tag="PW224_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908583.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22203..22403
                     /locus_tag="PW224_001898"
     CDS             22203..22403
                     /locus_tag="PW224_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2908584.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7746 a         3451 c         4641 g         6626 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa
     1081 cgttaggtaa agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga
     1141 ggggcttcca agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa
     1201 aggtcgctga ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa
     1261 ctcgaggagg ctatctgacg attcaatatt cattaattta aagtttgggt gactattact
     1321 aactattact tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa
     1381 ctctgaagtt acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa
     1441 aaatatcttt cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat
     1501 ttttagtctt ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga
     1561 agcgatacgg caagtgaaaa aatagggaag catgaaagaa tctacttaat ggcgcattga
     1621 ttcatgtgat agaaataaaa aactttttta aattatatcc tcatgattgt aacaaatcca
     1681 gtgatttgtt ttggtacaaa taaaaaaagg ccccgatgaa gttgtggcaa aagtggtttg
     1741 cctcaaaaat aagaaggaat ttccaaaaat cacttctcaa attttgccac atccccaatt
     1801 gggtcaaatt tgtttatttg aatatatttt ttacataagg atctttaata gttacattgt
     1861 cttttttcat cactttacgg attgttttat ccatgaattc cgtatcaaga gtttttgctt
     1921 cggtagcaat tttttctagc tcagatttgt atttgtcttt attagaacct ttatcctgtt
     1981 tttttaccat ttttactaga taatagcttt gctgataagt cgttggatct gttacaggaa
     2041 tgatatcaga aacttccccg ttttttagtt taaatgctgc tttttttact tcagtaggaa
     2101 tctctggatt ggttgaatca aaattcattt ttccatcagt ttcctttaat gttttatttt
     2161 ttccataagc ttgaacaagc ttggagaaat tttctccttc atttgcttct ttttttgctt
     2221 tatctgcttc ttctttagtc gaaaatagaa tgatctgtgt actaacttct ggatagaatt
     2281 ctttccaagc agtgtctaga tctttatctg ttaattttac attggctttc aaaccgtatt
     2341 ggaaagccag catctgttta aagagtttct tatatgtctc ttctgtttgg ccaactgctt
     2401 ttaattgttc cttgtagttt ggaccgagtt ttttgatttg ttcattatat tcttttgtta
     2461 cttcctttga agaaatctct tttccgtata catcatcaaa tgctttgaag acaatcattt
     2521 tttttaatag tgtttgatta tttgatttaa cttttgggtt atcataaaaa tcctgaactg
     2581 taagtgttcc acctttaaac gttgcaatcg tttcattgga cttgctacaa cctgagaata
     2641 gtaaaattgc acatgctgaa aggcacataa ttagtagttg ttttttcaaa aaatttccca
     2701 tcctttcctt attaaaaaca gtctaacaga agaattaggg agacagtctt tcattttcgt
     2761 aaggttgctg tagatattct tagttcgtaa aaatctattc tctctatgat ggagaacatt
     2821 tacatagttt tcacgagaca agaaagaaaa gggttgttaa acttaatata aaggagggat
     2881 gaaagatgat caggattatt cggttgattg gatctgtttt aatagtcttt gttggttttg
     2941 ttttaggaat tcttgtcaat aatgcacttg cggatatgtt tattggaaat agtgaatgga
     3001 ttggagcaat tgccggtacg gtcggacatt tagctgtttt tttactagct ttatttcttg
     3061 ctagtaaggt cgaaggaaaa agaatagaag attatggtgt ttctgttcga ggaaaagact
     3121 gggggtattt agcgggaggt ctcgttgttg gaattggagt gtttctgctt attactagtc
     3181 cattatattt gacgggggcg tatcggttag attcaggaaa cgcaaacatt gtgccgctga
     3241 taacgagttt tattttattt attgcagtgg gggcatcaga agagctttta ttccgtggct
     3301 tttttcagca tcagatgctt agatttggtc cgttaatagc aatgattgga tctgctgcat
     3361 tatttgctct tttgcacgga ttgaatccta acatgacatt tctggcagtt ttcaatatct
     3421 ttttagcagg ttgctttttc agcgcactta tctacagtac aggaagttta tttacggcta
     3481 ttggtgctca tattacttgg aattggacac aaggagctat tttgggtata ccagtcagcg
     3541 gaacgcaaga acaaggtttt ttctccactc tgatccaatc gcaaaataca ataattactg
     3601 ggggaaactt tggggctgaa gcatctatat caactacatt attcttgtta gtcttgattg
     3661 ttggtttgtt actctatgca tataaaacaa agcggatgaa gaaatacact gctgtttatc
     3721 aataaaagat gaagaaagca aggctgatat cggtcttgct tttttaagtc tgtcattgat
     3781 taaacgaaaa agtaaacaaa ttcaagcgaa tacacgacat cttttgttag attttcacaa
     3841 ctgactgtcg tgttctcatc attttcattt tatataattc ctttatcaaa tagaaggtaa
     3901 ttatatggag agtgatagaa atgaagaaaa cagcgaccgt attatttgtt attcttgggc
     3961 tgagtataat tgcagcaatg attggaatca gtacttctgc aaatgaacgg gcaaatgaga
     4021 atctttcatt tactgtgaaa actgatcgga ttgtttatga catgacacaa caagtcatta
     4081 ctattccagt caaaccaaac aaaagcgtga atgctagtga tgtacatgct gttctaacat
     4141 atggttggga tggtaatgga agttcagaaa aagttattgg ggaagtttat ttaaaagacg
     4201 tacaatggac cgctggtatt gagtatacaa taatgatttc tgcagaacta tcgattgatg
     4261 agatcaagag taaagataaa gttgatttga ttgtttttta tgatggacag atgacgatca
     4321 cggagaattt gaaaccaagc agttggacag ttgttggacc gccaagcagt acggatagca
     4381 caagtagtga aagcagtaca gaaaacacga gcggtgaaag cagtacagag agtacgagca
     4441 gtgaaattag cacagaaaac acgagcagcg aaagcagtac ggagagtacg agtagtgaaa
     4501 gtagcacagg aagcacgagc agcgaaagca gtacggagag tacgagtagt gaaagtagca
     4561 caggaagcat gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa
     4621 aagtaaataa tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg
     4681 cagggagcaa cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc
     4741 atcaagaaaa agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta
     4801 gaaccttgaa caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa
     4861 gctattttcg ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat
     4921 tgaataaaac gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg
     4981 aagttgtagc tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa
     5041 caggaacaca aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag
     5101 tatctctgaa aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa
     5161 gtctaaataa tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg
     5221 aaaggcaagt atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg
     5281 tgaaggatat cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac
     5341 aagcagaatc gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca
     5401 agatgataaa agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt
     5461 tagagggaac gattggttat attttactgg attcacttta caaaggtact tatcaatcct
     5521 tagaaaatct tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga
     5581 acttaatact attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat
     5641 agaaggagaa gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga
     5701 cgaaaatcat tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt
     5761 tgcgaaacat gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag
     5821 aatttcgttg caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa
     5881 caaatttgtg catccattga taacaaaaga agagtttacg cattacatac tagagccttt
     5941 ttttgaagca aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta
     6001 ttttatgttt ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa
     6061 agtacaagaa agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt
     6121 caagatttgt tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca
     6181 tttgtattca agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga
     6241 agacagagat tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag
     6301 tggggtagct cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat
     6361 ttttcaatac tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt
     6421 ctatattcaa tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc
     6481 tatgactgaa cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat
     6541 tagcgattat cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg
     6601 gaatggacaa cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag
     6661 gaatgagaaa aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat
     6721 tgtttatgcc aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga
     6781 ccagaaagca agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg
     6841 attttatctt cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt
     6901 ttcaataaag aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct
     6961 attctgtgaa caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg
     7021 ttgtttgtca attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag
     7081 ttctgttcga taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac
     7141 gagcagccag aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt
     7201 atgaaataat aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa
     7261 atgttgtcac gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga
     7321 aagaaggcac aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag
     7381 gggtatgttt tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata
     7441 aaagaatcaa aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg
     7501 ctcaattagt gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc
     7561 acagttattt tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt
     7621 tattaactga attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta
     7681 tagatacgac tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag
     7741 caacgacaac ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga
     7801 aaatttggga taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa
     7861 aaagcagaat ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc
     7921 ctgttctatt tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca
     7981 tataattctc ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa
     8041 gggagcgttg gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg
     8101 agagagcatt tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc
     8161 tggaaatgaa acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga
     8221 gacatctgtt actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga
     8281 agaagcaaca gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa
     8341 agagaatggg acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata
     8401 tttgaattac ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa
     8461 gtcaaaatta atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa
     8521 agagattatc aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa
     8581 cagtgtgttg atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac
     8641 aggcttctat ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa
     8701 aatgatggat gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca
     8761 tggcggattt cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta
     8821 taaggccggg tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga
     8881 ttatgtgacc tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt
     8941 tcgagagcgg ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat
     9001 gatgtctgta ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg
     9061 atttgcatca attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact
     9121 accggatgcc ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt
     9181 tatgggcctt ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc
     9241 tctacctacg attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga
     9301 tgatgtagta cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga
     9361 tcttttatta gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta
     9421 cacgaaacat gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta
     9481 tgtggatgaa aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc
     9541 ttatggagaa aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt
     9601 tgaaggaccg actgaaggga tttttaatga gacgatacca actattattt tctattacaa
     9661 aaaaatccca gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg
     9721 aaagaatgaa cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt
     9781 gaaagtacaa aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa
     9841 acattcgctt ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat
     9901 ttggcgaaga aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac
     9961 cagttatcat cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc
    10021 cgattagctt gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc
    10081 gaccagatga tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc
    10141 cttcaaagta caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat
    10201 taccgattct tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc
    10261 gtgattattt ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg
    10321 aacgatcacc ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag
    10381 cagctaaaaa agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt
    10441 cggcaggtgc acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa
    10501 aagctgatca tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt
    10561 ctggtctgtt ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt
    10621 atgaaaaatg tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca
    10681 aaagtttagc cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg
    10741 gaaatacaaa tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg
    10801 gggtgaaagt cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc
    10861 agtttgattt cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca
    10921 aggagtaaag tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa
    10981 ggagcaattt caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg
    11041 agttagagat ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt
    11101 cacgatctct tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa
    11161 gcaacaaatc cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag
    11221 aatgctggtt ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata
    11281 gcaaataaaa tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc
    11341 agcacctcct tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt
    11401 ttgttaggat gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata
    11461 agcaattaat gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa
    11521 taatagaaaa tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc
    11581 aaaaattcga tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat
    11641 aaaattacaa atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta
    11701 attcaccaaa atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga
    11761 aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga
    11821 ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat
    11881 tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc
    11941 tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa
    12001 atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa
    12061 atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat
    12121 cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa
    12181 gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca
    12241 atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc
    12301 gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata
    12361 ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg
    12421 aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt
    12481 tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc
    12541 atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca
    12601 ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt
    12661 gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga
    12721 ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg
    12781 ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct
    12841 cattttcata ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga
    12901 gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg
    12961 tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga
    13021 aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc
    13081 gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag
    13141 atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct
    13201 ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt
    13261 attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag
    13321 aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat
    13381 caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc
    13441 aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc
    13501 ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc
    13561 caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat
    13621 catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat
    13681 cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt
    13741 gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg
    13801 aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg
    13861 tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat
    13921 cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt
    13981 tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt
    14041 acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt
    14101 tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga
    14161 tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt taattgaacg
    14221 aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca
    14281 agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac
    14341 gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga
    14401 aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt
    14461 atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga
    14521 tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga
    14581 agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga
    14641 tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt
    14701 tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat
    14761 tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt
    14821 cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag
    14881 caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct
    14941 caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac
    15001 ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa
    15061 ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt
    15121 agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag
    15181 ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa
    15241 tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg
    15301 tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg
    15361 ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg
    15421 ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa caagattttt
    15481 tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga
    15541 ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt
    15601 tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata
    15661 aatacgcaag aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg
    15721 aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac
    15781 tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc
    15841 cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg
    15901 ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat
    15961 cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga
    16021 cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt
    16081 atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact
    16141 ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct
    16201 ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct
    16261 attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg
    16321 attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc
    16381 atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct
    16441 atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt
    16501 gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag
    16561 ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc
    16621 gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat
    16681 tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg
    16741 attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca
    16801 agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt
    16861 aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca
    16921 cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact
    16981 cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac
    17041 tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca
    17101 ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa
    17161 gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca
    17221 cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg
    17281 tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg
    17341 cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa
    17401 gacaagcttt cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga
    17461 cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact
    17521 atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg
    17581 aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact
    17641 tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat
    17701 taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg
    17761 tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat
    17821 ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat
    17881 ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta
    17941 caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt
    18001 tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag
    18061 aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat
    18121 ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg
    18181 taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat
    18241 tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa
    18301 tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc
    18361 gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta
    18421 tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt
    18481 atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc
    18541 atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact
    18601 ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata
    18661 acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag
    18721 catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa
    18781 cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg
    18841 atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca
    18901 gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag
    18961 atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag
    19021 ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc
    19081 agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca
    19141 atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa
    19201 tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg
    19261 aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca
    19321 tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca
    19381 tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca
    19441 atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa
    19501 tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac
    19561 aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa
    19621 aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg
    19681 tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat
    19741 gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt
    19801 gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta
    19861 gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat
    19921 atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa
    19981 ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct
    20041 cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata
    20101 cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc
    20161 gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact
    20221 tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat
    20281 ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac
    20341 ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga
    20401 tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc
    20461 atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg
    20521 gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa
    20581 gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct
    20641 atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat
    20701 gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat
    20761 ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa
    20821 atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca
    20881 aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat
    20941 caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt
    21001 atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac
    21061 aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct
    21121 atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc
    21181 agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt
    21241 tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat
    21301 caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt
    21361 aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg
    21421 atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta
    21481 tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt
    21541 atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta
    21601 ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta
    21661 tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt
    21721 ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata
    21781 ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa
    21841 ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt
    21901 tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca
    21961 aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa
    22021 tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat
    22081 gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg
    22141 cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat
    22201 ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg
    22261 gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa
    22321 ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag
    22381 caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag
    22441 acggtttttt tgtacgaaaa aatt
//
LOCUS       DANKMN010000053        22130 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000053 DANKMN010000000
VERSION     DANKMN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            581..1540
                     /locus_tag="PW224_001899"
     CDS             581..1540
                     /locus_tag="PW224_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2908585.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            1634..1828
                     /locus_tag="PW224_001900"
     CDS             1634..1828
                     /locus_tag="PW224_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908586.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            2097..2396
                     /locus_tag="PW224_001901"
     CDS             2097..2396
                     /locus_tag="PW224_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908587.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(2493..2927)
                     /locus_tag="PW224_001902"
     CDS             complement(2493..2927)
                     /locus_tag="PW224_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2908588.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(3159..3869)
                     /locus_tag="PW224_001903"
     CDS             complement(3159..3869)
                     /locus_tag="PW224_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2908589.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(3859..4719)
                     /locus_tag="PW224_001904"
     CDS             complement(3859..4719)
                     /locus_tag="PW224_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL2908590.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(4724..5362)
                     /locus_tag="PW224_001905"
     CDS             complement(4724..5362)
                     /locus_tag="PW224_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL2908591.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(5377..5676)
                     /locus_tag="PW224_001906"
     CDS             complement(5377..5676)
                     /locus_tag="PW224_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2908592.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(5719..7203)
                     /locus_tag="PW224_001907"
     CDS             complement(5719..7203)
                     /locus_tag="PW224_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2908593.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(7223..7684)
                     /locus_tag="PW224_001908"
     CDS             complement(7223..7684)
                     /locus_tag="PW224_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2908594.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(7702..8769)
                     /gene="ulaG"
                     /locus_tag="PW224_001909"
     CDS             complement(7702..8769)
                     /gene="ulaG"
                     /locus_tag="PW224_001909"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL2908595.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            8987..9757
                     /locus_tag="PW224_001910"
     CDS             8987..9757
                     /locus_tag="PW224_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2908596.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(10030..10857)
                     /locus_tag="PW224_001911"
     CDS             complement(10030..10857)
                     /locus_tag="PW224_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2908597.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            11425..12660
                     /locus_tag="PW224_001912"
     CDS             11425..12660
                     /locus_tag="PW224_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2908598.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(13659..13919)
                     /locus_tag="PW224_001913"
     CDS             complement(13659..13919)
                     /locus_tag="PW224_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2908599.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(13946..15247)
                     /locus_tag="PW224_001914"
     CDS             complement(13946..15247)
                     /locus_tag="PW224_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2908600.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(15539..15997)
                     /locus_tag="PW224_001915"
     CDS             complement(15539..15997)
                     /locus_tag="PW224_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2908601.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(16010..17341)
                     /locus_tag="PW224_001916"
     CDS             complement(16010..17341)
                     /locus_tag="PW224_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL2908602.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(17352..18794)
                     /locus_tag="PW224_001917"
     CDS             complement(17352..18794)
                     /locus_tag="PW224_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2908603.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(18781..19545)
                     /locus_tag="PW224_001918"
     CDS             complement(18781..19545)
                     /locus_tag="PW224_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL2908604.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(19649..20524)
                     /locus_tag="PW224_001919"
     CDS             complement(19649..20524)
                     /locus_tag="PW224_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2908605.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(20779..20863)
                     /locus_tag="PW224_001920"
     tRNA            complement(20779..20863)
                     /locus_tag="PW224_001920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20827..20829),aa:Leu,seq:caa)
     gene            complement(20881..20952)
                     /locus_tag="PW224_001921"
     tRNA            complement(20881..20952)
                     /locus_tag="PW224_001921"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20918..20920),aa:Gln,seq:ttg)
     gene            complement(20962..21034)
                     /locus_tag="PW224_001922"
     tRNA            complement(20962..21034)
                     /locus_tag="PW224_001922"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20999..21001),aa:His,seq:gtg)
     gene            complement(21096..21180)
                     /locus_tag="PW224_001923"
     tRNA            complement(21096..21180)
                     /locus_tag="PW224_001923"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21144..21146),aa:Leu,seq:caa)
     gene            complement(21201..21271)
                     /locus_tag="PW224_001924"
     tRNA            complement(21201..21271)
                     /locus_tag="PW224_001924"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21237..21239),aa:Cys,seq:gca)
     gene            complement(21317..21388)
                     /locus_tag="PW224_001925"
     tRNA            complement(21317..21388)
                     /locus_tag="PW224_001925"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21354..21356),aa:Gln,seq:ttg)
     gene            complement(21400..21472)
                     /locus_tag="PW224_001926"
     tRNA            complement(21400..21472)
                     /locus_tag="PW224_001926"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21437..21439),aa:His,seq:gtg)
     gene            complement(21475..21548)
                     /locus_tag="PW224_001927"
     tRNA            complement(21475..21548)
                     /locus_tag="PW224_001927"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21514..21516),aa:Trp,seq:cca)
     gene            complement(21555..21635)
                     /locus_tag="PW224_001928"
     tRNA            complement(21555..21635)
                     /locus_tag="PW224_001928"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21599..21601),aa:Tyr,seq:gta)
     gene            complement(21637..21712)
                     /locus_tag="PW224_001929"
     tRNA            complement(21637..21712)
                     /locus_tag="PW224_001929"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21677..21679),aa:Phe,seq:gaa)
     gene            complement(21748..21820)
                     /locus_tag="PW224_001930"
     tRNA            complement(21748..21820)
                     /locus_tag="PW224_001930"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21785..21787),aa:Asp,seq:gtc)
     gene            complement(21830..21902)
                     /locus_tag="PW224_001931"
     tRNA            complement(21830..21902)
                     /locus_tag="PW224_001931"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21867..21869),aa:Val,seq:tac)
     gene            complement(21918..21989)
                     /locus_tag="PW224_001932"
     tRNA            complement(21918..21989)
                     /locus_tag="PW224_001932"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21954..21956),aa:Glu,seq:ttc)
     gene            complement(22004..22093)
                     /locus_tag="PW224_001933"
     tRNA            complement(22004..22093)
                     /locus_tag="PW224_001933"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22057..22059),aa:Ser,seq:gga)
BASE COUNT         7038 a         4554 c         3530 g         7008 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttaccttcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
      541 aaaatagttc cgtcgaaaga attataagga gtgagtacaa atgacctata cccatcttac
      601 accaactgaa ctcgtaatga tagaagctta tttcaatcaa tcacactctg tttcaaaagt
      661 agcccatctg gtacaacgtt ctcgacaaac catttataat gtctatcgtt ttcttaaatc
      721 tggtggaagc gcaataagct actatgaaca atataagaaa aacaaacgta actgcggaag
      781 acgtccgatt gtgttaccag atgggcaaca agaatacatt caaaaaaagg tggctcaagg
      841 gtggactcca gatgtcctta ttggtcgagc agagtttcct attgcttgtt cggtacgtac
      901 actatatcgg atgtttaaag ataaaacctt tgaccctcac gatttaccaa tgaaagggaa
      961 aagaaaacca aacggacata aagaaaaaag aggaaaacaa gcttttcgcc gttcgattca
     1021 ggatagaaca actgattata tacagttcaa tcatgagttt ggtcacctag aaggtgacac
     1081 cattgtcggt ggaaaacata aaagtgcggt gatcacgtta gttgagcgat tatctaaagt
     1141 gatcatcacc ttgaaacccg ctggtaggca agctatagat attgagaata gactgaatca
     1201 atggtttcaa tctgtaccga aacacttgtt taaatccatc acatttgatt gtggaaaaga
     1261 gttctcgaat tggaaaaaga ttagtaatac caatgatatt tcgatttatt tcgctgatcc
     1321 aggaacacct tctcaacgag gactaaatga acattcgaat ggcttattac gtaaagatgg
     1381 cttatataaa gctatggatt ttaataaggt atccgaaacc tttatccaat cggtggcatc
     1441 aaaacgaaat catattccta gaaaatcatt acactatcgc acaccattgg aagtattttt
     1501 gagttacgta ggtaaagatg atttgtctag cttaatttga caaacgaaat atcataaaga
     1561 tatagataca taatcatttt cacttacata tatttgaata ttaactaaaa acagcaagaa
     1621 agaagaggat caaatgtatt atgtagaagt aaaaacaaaa ggcgtcaaaa acaaacaata
     1681 tgtgaaaggt atcagtaacg aatatccttt acttggttca tggaaagaag ctgctccttt
     1741 ttccaaacca tgtgccataa aaataaagaa cgagctagaa aaagaactga cttgtggtaa
     1801 agctgttgta gatatcatcg aaaagtaaat ccaatgtata gaaaggaaga tcacacatga
     1861 aaagcgacgt acaaattgct aagattatag aattagaaga agaagacgca gtgttgacaa
     1921 ttgaaaaaaa ataaatgttt gtataccata tgattatttc aatatgtatc ctggaattgg
     1981 agatctcgta gagctatatc aagatgaatc aaatatactt gtcattcatc atgaaatata
     2041 gagacttata aaattgttta tttttataat gtatcagtat agaaaggaaa taaaacatgg
     2101 attatactgt tgaatttgac agtgaactac ttaagcaatt tccacaagaa gaacatttca
     2161 tgtataaaga agcaatttcc cacaaagtga cagagtattt ggagcataat ttttttcgta
     2221 taaagccagt gcgtactcgt tcggcaaaag gaatatacga gatgaaagtc aaaattaata
     2281 aaaaaaatta tcgcatagca tttgctttaa aaaaaaatca tgtgttcatt ttttatatta
     2341 gttcgaattt gcaaaaaatt gtatttgata aagaggtgag taaaaaaata aaatagctga
     2401 gcattggagg atagattgag tgatactaag tctatcctct tgtttttcta aagaactaag
     2461 ctagaatttt ttcttttgta cgttattata tgttatttgt cattaccaaa aagcaccttt
     2521 tttcttctag tatctatgac ataagaaata ccacccattt cctttacttg aacaaatcct
     2581 actgattcat aaaatttttt tgttttctca gtatcatctg tcaataattc gatttggact
     2641 ggtccataac tagtgatctt ctccaaagct tcattcaaaa gcactttgcc tattccttgg
     2701 cgctgatacc tcggatgaat gaggatatct tgaataaatg cgatcgtcgc aaaatcagtg
     2761 atccaacgaa cgattccgat aatcttccct tccccattac gctttatcag agattcagat
     2821 tgttcaaatg ctctagctaa ccttgcagtg tcttttgtgt aattcgtcca accaacgctc
     2881 ttatataatt cagaaatctc atcgatcata agggaatcaa tattcatttt tttcttcctt
     2941 tcttatacaa ttctccctct aataatacag ttctttgact atttaatcta tcacatataa
     3001 ctctcctgta aagattctct cgattcctta aaattcctct ttccttttag aatgcatact
     3061 ttttgataaa taaaaaggtt gtgataaatt ttcttcacaa cctctgtttc tctctatcaa
     3121 gtgttatctt ttaattttct acccttaccc tagcatctct attgcccgta gtatgcatac
     3181 tttccatgtt tgcgaaaata atgtttatct agtaaatatt gcgggatttc acatatattg
     3241 gggttgatca gctcgttttc tttggccatc agacaaattt catctaagat caaactgttt
     3301 tctactgctt tcataggtga atccccccat gtgaacggtc catgtccata gacaagtact
     3361 ccagggactt cgtttggatc taattttctt tccttaaacg tctcgacaat cacattccct
     3421 gtatgaactt cgtaagcttc tcttacttct tctttcgtta attggcgtgt acaaggaacc
     3481 ttaccataaa aagcatcagc atgagtcgtg ccataagctg gcaaatcgcg tcctgcttgc
     3541 gcccacatga cggagtgcgt ggaatgagta tgagtaatag cagttatatc ttcaaaagtt
     3601 tgatacaaaa caacatgtgt ggggagatct gaagatggtt ttaagctatc ttcttctatt
     3661 atatttcccg acaaatctgt taccaccatt tgatctgctt gcatgcattc atatctgaca
     3721 ccgcttggct taatgacgat gactcctagc tcacggtcca cttcactcac attgccccaa
     3781 gtcaatttga ctaatccgta tcgcggcaat gccagatttg cttcaaggac acgttgcttc
     3841 attgctgcaa ttttttcttc acaagacatc atagccagcc tccttcaatt tagaacttaa
     3901 gtagtctttt gcctgctgga tttctcttcg aaagtcttgg cttttttcac tccacatttc
     3961 aatcagaaat gttccatcat aatctagacg tttcaaatgt cttaataatc caagaaaatc
     4021 aacacaaccg ctaccaaaag gaacatcacg aaactggcct gaatattctt ttgttacagg
     4081 gtaagtatct tttaagtgga tagcagtgat ataatcgatc cctttctcaa gttctcgagc
     4141 cggattattt tccggccatg cggacaaatt gcctaggtct ggatatactt gtagaaaagg
     4201 tgaatgaatc tgttctttga tttctaaaaa ttttgaaatg gagttcataa atggatcatc
     4261 catgatttcc acggaaagaa tgatgccata tttagaagct tcctttacgc tttcttttat
     4321 tccttcgata aagtcttctc tggaattcat tgatttctct tcataataaa catcgtatcc
     4381 agctatttgg attactttga cgcttagatt atgcgccaaa tggattgctt ttttcattag
     4441 ttgtttcgct gcttttttct tttctggatc agcagaacca aacggaaatc gccgatgcgc
     4501 acttaaacaa atcgaatgga tacgtacacc ggagctgaac atctcttctc tcagctcagc
     4561 tatcttttct tcagaccaat ctaatcgtgc taaccgctca tccgtctcat caatcgacat
     4621 ctcgataaaa tcgaagtcca tttcttttac aagggaaaaa cgttctttcc atgaaatatc
     4681 tttgggtaac gctttttcat aaatcccaat ttgagccatt tccttacccc cagatacgaa
     4741 tgatctcatc tttgaagttt tgtgcggcct tcatcggatc atttgctgct gtaataccac
     4801 gaccagtgat aaaggtatac acatcgatcc cttcaaataa gtgtaatgtg tgtggattta
     4861 atccaccagt tacagaaaca ttaaagccta gttcaatcag ttttttgatc ttcgataaat
     4921 ctttttcacc ccaagtttca ccagataaca gcgcatcacg gctttgatgg tagattacct
     4981 gccggatacc gatgttatgc caatccatgg cttgttcaaa agtccaattg ccgtagagtt
     5041 ctacttgcaa ttcacctatt tctttttgtg ctgcctccat agttggaatc gttgcacaac
     5101 aaatgactgt catccagtct gctcctgctt ttgcaacatt actcgccact gttcctccag
     5161 cgtctgcaca tttcgtatca gcgactagtt ttttatctgg gaacatactg cgaatacagc
     5221 gaatcgcttt ttgtccttcc tgtaagcaaa gaattgtgcc cacttcaata atatctacta
     5281 catcaccaac tttcataaca tctcctaatg catgttctaa gtcattgtga tctaacgcaa
     5341 tttgcaaatt cggcctgctc atcttagttc cttcctttac ttgttttttt ggtaccactt
     5401 tgtttcagca aatttttcac tgatttcttt gactgacatc acatttttga taccgataac
     5461 tgtaactcct tttttctctg cctgagtaaa catattcaaa aaattgactg gcgtaaaaac
     5521 tacatcgtat tggccagctg aactttttcc ctctgaaatg gcacaatgat ggattttggt
     5581 aattggtacg tttagttctt tcattgcttt ttccacactt ctcatcatca tcaagcttgt
     5641 tcctgatcca tttgcacacg atactaaaat tctcatctta atccctccat tatgagtgtt
     5701 gtctaatcag attttctttt atactctctg cttttctgca tatgtgtcat agtcttctac
     5761 aatcaaaaaa taaccatcag gatttttccg atattgtaac tgcggaatag ccaataaacc
     5821 aatgacaact acggcaattc cagcataacc taggactttc atgattactg taaacaatgg
     5881 ccaagctgtt gcccagtcga acatcccgat ataaccaccg tatgttgaca tcccaatcca
     5941 tgtagcgaat aatgctgaac cgcctacttg tatgattcct gaaagaaacg ggaacaggca
     6001 agcggctttg aatccacctc ggttattggc ataaactgct ataacggcat tatcaaagaa
     6061 caacggaatg aaaccggcta tgacgatcgt tggtgatttc aataaaatca gtaaaccaat
     6121 catcaaaaac tgtcccaaag caccaaacaa gaacccaatc gttaccgcat ttggcgaacc
     6181 aaagccaaaa gtagcagcta cgtcaattcc tggaacagct cctggtaaaa tcgtgtttga
     6241 gattccttgg aaagattgag ttagttcatc gacgaatgtc cgaacaccta attgcaagat
     6301 cgccagatat accgcaaaat ttaaagaagt cgtcataatg tagaataaga aactctgccc
     6361 ttcttgcatg aattgcgctt gaatcaaata atctttgcct agtacaatca gaatgatccc
     6421 aaagaaaaat agcatgagta acgaagtagc aaccatattc tcattgaaaa tcgacaagaa
     6481 tcctggtaat ttgatatctt caattttttt gtcttgcttg attgatctat ttttcttttc
     6541 atcccgcttt ttcatccact cagctaattt tgcaaatata aagattccga acatttgctg
     6601 atgtgccact gcaaatccgg caccttctgt caattcctgc gtgattccta ctgttaagtt
     6661 agaagctact gtccaataac aacctaaaat cagtcccata aaaatcaatg cttgtatcct
     6721 acctagatca ggaaagcaaa acagtaaaat ccaaaaagcg gtagctgctt gttgaacctg
     6781 aacgtttccg gtagtgaaaa cggaacgcag tttcgtatat ttctgaaaac ggactaataa
     6841 gatatttaaa acaaatgcaa tcagtaataa aatcatcaca tctccaaagg tacggccaaa
     6901 tgtctccatt aatccagcat ccacagcatt ttgtccaaaa taaggatctg taaccatggc
     6961 atccaaattg aaccgttcct ttagtccaac caagattggc cgaaaattat tgaccaaacc
     7021 accagagcca acagtcaaaa taaagtaacc gaccgttgcc tttaagaatc cagcgatgca
     7081 ttcatgaagc gggcgtttca gcaaaatata gcctaataac acgataaaac caatcaggta
     7141 tgctggctgc gtcaagatat ttgttgcaaa atacgtccaa atgcttaaca aaaattcccc
     7201 catctttttt ctcctctatc aattaattat attctgccat cacaacttgg taatcatcga
     7261 tggagttcgt agagatgaga cgctcaacca atccatctgt cattaataat tcggataatt
     7321 tttggatatt ttctagatgt tcttctggat cttttgctgc gagtgtaaaa aacaaactag
     7381 ccgttttctc ctcattctcc ccttcacttt caaaacggat tggctgtttc aacttggtaa
     7441 atgaaatagc tgttccaaaa acaccatcac ttttttctga agaatgcggc attgctactt
     7501 gaggtgcgat cacaatataa ggaccgtatt tttctacaca atggatgatc tcctccacat
     7561 acgtttcatc aataatttcc ttatcgatca atacaagaca gctttcggcg atagcttcac
     7621 gccaatccgt gatttcttta tctgggtaat gaatcaattg atggtcaaaa aagtatttca
     7681 gcaagaaact ccccttcttt cttataaaaa cgacggaaac ggtgtatcat tttcaatcga
     7741 gaagacatct tcaaatcctc gataaaaatt aaactcaaga tcatctttgt ttgtcggata
     7801 gatgaatttc ccgcctactt gccagatata aggtttaaat ccatacttca ggcgatcctt
     7861 tttgaatttc caaatttcag tgatctcttt tggatcagcc ataaaattag accaaatatc
     7921 gtagtggaca ggaatcacta cttttgcatt caatgactcc gccatgcgca gcatatcaac
     7981 agaagtaact ttgtctgtta tccctcgtgg gttttcacca taggccccta aacagacatc
     8041 gattttgtgt tcattaccat gtttggcaaa taaattggag taatgtgaat ctcctgcatg
     8101 ataaagattc cctcctgatg tttcgaataa atagtttact gcaatttgat ccatctcctg
     8161 aggcattttt ccttttaata cctcttctgg atcttcacaa gtgaccaacg ctgtccgatc
     8221 aaacgcttct aaagctacta cattgatgtc tttgattttg actgcatcac ctggctttac
     8281 tgtaatcgtt ttttcttcag gaattcccca tttttgccac gtgcgaacca cttcttttgg
     8341 cccaatgaat gttgcttcag gacaattttg atgaactgct gcggctgtat ggatatctag
     8401 atgatcggaa tggatatgtg taacgattaa agcgtctaca ttttttatcg caaaaggatc
     8461 gattacgaaa ggctgagtac gtaaattagg ctgcatattt tgaacgccac tcatacgcat
     8521 catctgatgg ccttttttca tcatgccatt cccatgacta cgttttcccg tcccacacca
     8581 tagatcacat aaaatattcg ttccttcatg acttttcagc caaattcccg tacagcccag
     8641 ccaccacata gcaaaagttc caggatccac tacttctgtt tcgatttctt cattcaagta
     8701 tgtgccccac tcaggaaatg tttctactaa ccacttttct tttgttactt cattgacagt
     8761 tttactcatt tgcttgctcc ccccatatcg ttgttccaac ttacaagtaa atattaatgt
     8821 gtatcagcat ccatgaaaac ccttttatat gaccgtttgt tttttatatg attttctttc
     8881 agtaatcaat gattgtttca gtataataaa gacagatatg aatgtttaaa tgaacaacta
     8941 gtcaaataaa tccatggatc aagatgtccc tcaggaggca aaagtcatga aaaactcact
     9001 tgtcaatatt gaaaaacgtc aaaaagaaat tttggaactg cttcagcaga ctgaacaact
     9061 atcaactttc gaattagctg aatcccttga tgtatctatt agtacgatac gaagagattt
     9121 aaatttatta gaagaaaaaa acgagatcat acgcaattac ggttattgtt cttataatta
     9181 caaaaaccag actgattttg atcaatctgg tcctgaaagg attaaacaag cgattgccag
     9241 agaagctagt aaatacatta acgattatga tacgctgttt atcaattcta gctcgactgc
     9301 gttgaaaaca ctggattttc ttcaatctaa ccatttgaca gtcattacaa acaacgtgaa
     9361 aatcaattac tctccgcaca aagataattg tagttatatt ttaactggtg gagagatgcg
     9421 ctttccaaaa gaagcactag tgggcgacat cgcattaaat acgatttctt cagtcaatgc
     9481 agacatttgt atcattggct gtagcggagt cgatgtagta catggggtca caacaaagat
     9541 attaaatgaa tcaacgatca acgaaatgat ggtcaacaag acaatcaaat gtaagatttt
     9601 agtcgctgat catcgaaaaa tcggactgac atcaaagttc aagattgctg atatttcagt
     9661 ctttgattat ttgatcactg ataaattttg ttctgaaaaa gtattagagg agatcagaaa
     9721 tacggggata aaaaccattc aagtagattc attttaagaa ccaggacgta aatatgtaaa
     9781 taggattatc aaaaaaatga ataacaaatt tatcgataaa tgaaaaacac gctacgctca
     9841 aattgataaa ggaatcccaa gatgtatcca tccaatcgat accatcataa tctattgctt
     9901 cttttataac cgttatatgt tgcctaaata ggaatcaaac aatatcgaaa aaaggaggta
     9961 tgacaaaagt cacacctcct tttttcgaat aaacggtatt caaatcagaa cactgatttc
    10021 acaaccactt tatttcaggt cgttttcaat cacctttacc gcgtaatcat ttccgttgaa
    10081 acgcgctatt ttttttgcgt ccatatatct ttctaaataa tcatcttctc ctaattgctt
    10141 atggcaagaa gcaatttcat aaagcaagta ttccaatgca taagtcgatt tatgtttctt
    10201 attatactca atacccttct cacataattg gattgcttta tcataatcct cgattttacg
    10261 gtaataggtg gcgatatcat aatatacaat gcagaccatc agattatcaa aatcagtctc
    10321 actcaatatt ttaagtgtac gctccacata aactttcgca aaatctaatt ctccttttga
    10381 caagtagatc attgccacgt ttgcagctgc ttgtaagtga tggatatctg tttcggtcgt
    10441 ttgttccaac acttgattaa aataaaataa tgctgcatct ggttttttcg cataaagatg
    10501 atcaagcagt ccttgataaa ataaaatttg atttaaaaga tcagaattac ttactttttc
    10561 ctgatcgatc gtttgtaaaa ttttataaga ttcttggtat tttgatacag aattcaatga
    10621 ttgaatctca tttatttttt tcctgatttt gtcttcttct gtttctacta atatatcgga
    10681 gatctcgaca ttcagctttt tgcaaagcgc acttaagata ttgatgcctg gcgacatatt
    10741 cttgttttcg atattgctga cagttgcttg ttcacaaatg ccttctgcta attgtacttg
    10801 tgttaacccc tttttctttc ggtattcttt tagtaatgct ccatctactt taatcaaaac
    10861 aatttccttc cctttaaaaa attatttctc ctttatatca tacacaaaaa aaagttctaa
    10921 acaaccaaaa atgtttagaa cagaataaaa attttaattt taaaataata ttcaaattaa
    10981 acaactggcg tatctgctcc ttgatttctt tttgtgttat ctgaataatt atcgtttaca
    11041 gttataacac atagtgaaaa ttgtcatggc gagaaagtct aagaatattt ttaaacataa
    11101 ctatttcttt cttacgtgtg tataatgacg acagcacacc tatcaattaa tataattatt
    11161 ttattttttt atgttctcat gtttattttt aatgacacaa cttctcgtat ttatctggca
    11221 caacatataa atcgatcaaa aaattgttgc gtgttttttt gaaaattgga gtttcaactt
    11281 gattacaaaa tttaaattta gaagttgtga aaacagcgag agacagttta atattcaaaa
    11341 tatacatagg ctactcgaac gctgtgtttt ttagtatact agtaagaaac gaagggatag
    11401 actcattcca ataaggagga tttcatgaag acaattcttt ttgctcctgc agtattcaat
    11461 ttagctgaga caacacgaat gattgaggtt gccaaagcta tgaaagaccg ttattactgt
    11521 gtattctttg gcttttctaa aacttttgct cctttgatca cagaaagtgg ctttgagtat
    11581 catttactag cgccgatcct tacgaaaaaa caagaagaac aaatcataaa atttgaccag
    11641 ttgcgtacac ttaaaaaccc ttttaccaaa gaaatggttg cctctcgtat cactagtgaa
    11701 cagagtctga ttgctaagtt tgagcctcga ctgattgtga caggttcgaa tgtgacaatt
    11761 tttttatcgg cccgcaataa aaagattcct ttagtatatg ttaaacccta tgctttgagt
    11821 gaaccttttt ttcaatcaga gcatgcgctg atgccccacg agttacgttt cttcggccct
    11881 ttaagcaatc ccctttggca ctggacaaaa aagcgtatag taaatacccg atggatcccc
    11941 aatgccttca aacacgctgc acaaaaagaa atgcttcctt taccaaaaac aagtttacaa
    12001 atgatggatg ctgatctaaa tctcatcaca acgcctaaac tatttttgga agaagaacag
    12061 ctgccggaaa attatcgaat cgttggtcca atttttgcta agctcagtac ttccttgcct
    12121 gaagaagtgc taacttttat acagcgaaag caacaagaaa gaaaacgtat tatctatttc
    12181 gcaatgggta gttcaggcaa tccgcgtatg ttacgcaaaa ttttgttctt cttacaaaaa
    12241 caaccggatc tagcggttat tgcacctatt gactatctgc ttgatgacat tcaatctgac
    12301 tcagaagatc ttttaataac agaatatctg tctagtcatc ttttgcatga attcattgat
    12361 ttttctttta tccacggcgg ggaagggacc gtccaaactg cctgtgaatc cggcaaaccg
    12421 tttatcggtg tggggatgca atttgagcaa agctgtaata ttgactattg tgtaaaatac
    12481 ggaaacgcaa tcgccctcac aaaaccagtg actgaaagaa aactgcagat tgctctgcgc
    12541 gaagtttctt cacccaagat tcgtcagcag gcacgcacgc tgcaagccgt ttttccaata
    12601 aatggtccac aacaagcaat tcaagaaatc gaaaccttcc tgagcaacca cccaatatag
    12661 tttcaagtag aaaaagcttt tttaatagag actcatcagt aatttcgcta ttcgagaccc
    12721 agacagtgaa aatacaaaaa aagtaaatag tggattgaaa agttagagtt acttaaatta
    12781 cggtgtctaa aaaacacaaa atcatcctta aaataaaaga tctgacgcta tccaaatgag
    12841 cataggtagc gtctgccatt tattcttagt caaattcttt gacacctttc tctggttgga
    12901 tcatctcaac gtttcttcaa aagaaagacc ataataatta ggcaaataca ttttcttaaa
    12961 atgtgtcata tagatctaga agcatcgctt tgtagagatt tgtgccgcta gtcccctcaa
    13021 caattactaa atcaaatgta ggatttacca ctttatcttt gataatcaac ctccctcttc
    13081 tgttgagaca atgatctcgc aatccgatgc atactcctat ataaactgcc agcaatcccc
    13141 acatattgag tagatcatgt cagaatgttc tatgtcgtca gcaacgatac actaaaagtc
    13201 acgttcacca tgactgattg ctattcccag cacaattatt tctatacacg tttcatgatt
    13261 tgaataaaca ctcacatcat acatattacc attcatacac catactgcag cccctactat
    13321 atggttgtaa gctcccaaat ctatatttga cttgatcact ttggtagtag atctactaat
    13381 tctaaatcca ttgctgataa aatttccatt ttttcctcct tctttctgcc aaaacagtta
    13441 aaacaataat aatctttatg taaaaaaggg ctgtgacaaa agccttgtca cagtcccata
    13501 ttccgaataa acggtggtca aaagcttgca tctgcggtaa taagttcaac caaacgaaaa
    13561 ataagccaac atttcacaaa aatttaagaa gcaattttcg taaatttctt cttatttctt
    13621 gaagctaacc acttctgtta caaccacttt tagaacattt aaagcttcga tgtgacaaac
    13681 catctatgct gctcgatcat aaaattgata gcaaaaattc cacaaagtac atatgtcccc
    13741 catgaaaaca aaacattcat atgcggtaca atgatctttg gaagaatcac gatcaaaaat
    13801 gatgccagtg cccaaatcca tcgattgatt tgaatatgat atgtagctag catattcaat
    13861 ataaggtgac tagccgttaa taaaaagata aaaacaaaaa ataaaatcag ccaatacatt
    13921 gtttcccacc ttctaaaaac caaacttaga cagtctggac ttcgttttct ccacgacttt
    13981 gttcatctga cagcatcttt ttatcataag cttttacaaa tggataatag ataataaatg
    14041 ctatcaatgt gttgatcatg atcaagataa caacacgcca gtcatttcct gctgaaagat
    14101 aaccgccgat tggagctggt aatgtccatg ttggcaaaac agtaaatcta ttgaccattt
    14161 ctaatttgac tgcaaaataa ctgataatag tcaaaactaa aggaactaag ttgaacggga
    14221 tgaacatgta aggattcaac atcataggag ctccaaaaat tatgggctca ttgatattga
    14281 agataccagg aataatagaa aaacgaccaa cctctttcaa gaattttgat ttacagaaga
    14341 atataaacaa aacagatacc acgatcgttg ctcctgcacc accaattgtt accgtccatt
    14401 gataaaactg ttcaggagcg atatagggta attgggtggc agctgtacca tctgccaatg
    14461 cctttgcatt ctcatccaac atgatcagcc acattggacg aacaacagcc cctacaatac
    14521 tcatgccatg gataccaaat gaccacaaca aatggatcaa aaacattggt agtaaaacac
    14581 caagcaaatt attccctaaa atattggtta aaggctcgaa taacgataat aaggcagcgt
    14641 ttacatcaaa gcccgcgacg cttcgaatca gccaagtaat tgttaaaatg actgcacctg
    14701 gaatcaatgc ctcaaaacta cgggcaacag caggtggcac ttgttctggc attttgatag
    14761 taatatttcg atttttaaag aaacgatata cttccactgc aaaaatcata gataagatcc
    14821 ccgcaaacat cccagatgct cccaaagaac tcataggtaa gacccagcca agaggtgcct
    14881 cttcaggagt taaaacgttt acaggaactt gtaacataaa gaacgttcct aaagataata
    14941 taccacctgt cacactatct aatccatatg atttagctag atccgaaccc ataacaaaag
    15001 ttgcataaat agccatcaat cccatcgtca tcctaaaggg taaaacaatc gttgccgaat
    15061 aaggggcaat aaattcattc catccaggaa acggtaaatt gataaagacc ataaaaaatg
    15121 tcccaatcaa aatcaatgac aatgttgaaa caaccccatt acgaattgct aataaatgac
    15181 gctgtgtacc aatttttgcc attggtggga caagtttctc ttcgacccat ttcatcagct
    15241 tttccactaa aaccactcct tttcaataaa atatgaacca ttcattgcat gacaacgcaa
    15301 agaatgttca taactaatta tataattaag aaattttatt tcaatatatt ctcttaaata
    15361 atcaaattta tttttaagaa tcctgaaaat aagttttcta aatcatagga gcaacaaaaa
    15421 aattcgataa ttaacaaaaa tcaaaatgat taaatctagt gattgaaaca tatcatttca
    15481 aaaaaatttt gtaaattcac ccataaatat caccctattt aacttttttc atcgtacact
    15541 aaaattcaaa acaactatct ttaatcttta cttcttggtt ttgttttaat aaatttcctt
    15601 caaaaatgcc tggagaaact acaatgatca ccgtatcttt ttgtgcttgc tccaacttct
    15661 tcaaatctat aatagctaac agtgtccctt cttttacctc ctggttactt tcaacccgta
    15721 tctgaaacgg acttccttcg agttcaactg tatctaaacc catatggatc agtatttttc
    15781 tgccagattc actttcgatc gttatggcat gtcgggtagg aaatatatta tcgattattc
    15841 ctttaactgg cgcatggata tatccatcat gattcgttat agcgtatcca actcctatca
    15901 tttttgaaga aaagacctcg tcttttactg ctgttaatgg aataatttga ccactagcta
    15961 cagcttttag aatgtttctt gttttaaaga aatgcacttt ttatcacctc tactttaatg
    16021 ctggccaata ttttttgttt actttgatca tttcatctag tatttttttt gcgactctgg
    16081 catttggtac ggtttgattc aatgtaaatg cttgtaaagc tttttgataa gaaccttcaa
    16141 agtaagcatc tactagtaac ttctctactg caacttgtgc ctccatcaat cctttatgaa
    16201 aatcactgat tggttttctt agtgtaattg gctccacacc agttgcacca acatatgccg
    16261 gtatttcaac aactgcatct gaccgtagat tcggaatcgc cccttgatta ggaacgatta
    16321 gcataaagcg acgacgatca tcattcaaca aggaaatagc tatatcgacg atatattgtc
    16381 catgctcccc aaaattaaag ttcaatactt cttccacaat accgcggcgt attttatcag
    16441 ccatttcttt ggtatttttc tctcgtccat ccatgaccat attagcacgt gtatactctt
    16501 tgtcagtatg ctcaacgacc atatcaggat ataagtaata ctctaagtaa ttattcggga
    16561 tattacttgg aaaatttttc gtgatgactg acatcatttg aaaggttttt tgccaagttt
    16621 tatctcctat attaaaatcg gcgacttgca tttcttttgt tattagtttt tcaataatct
    16681 ctggcataat ttctcgttgc aattctttat cataaatcga tgtataccag ccaaagtggt
    16741 ttaaaccata atacgtaacg atccaattct tacgatcata accgtaattc actgcaatcg
    16801 tttcttcaat tgaaatagtc atatcacagg cattaatcat ttttacattc ggatatttac
    16861 gacgaacagc ttcagaaacg atcgactctg gatttgtata attcaaaatc catgctttgg
    16921 gggcatagtc ttgtatatga tcgacaattt ccaataaacc tttgattgag cgcatcccat
    16981 aagcaaatcc tccgagtcca cacgtttctt gtcctactaa tccatgttta agtggaatct
    17041 tttcgtcctt ttctcgcatc tccaatcctc caacacgaat ttgtgagaaa acaaaatcgc
    17101 atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat gcgaatattt gaaaaacctt
    17161 ttcttttcag cataaattta acaatcaagt acatatccat atttctttct tcatcaatat
    17221 catataaacg tatttcagat aatggcaacc gtttttcatt gttcaaaatg gcttgaacga
    17281 ttcctggtgt atacgtactc ccgcctccag ctatagtgat aatctgtttt ttcatcatca
    17341 tcttttcctt tctattcttc taataattta ttgaattctg ccataacata agtcacttct
    17401 aagccgatga taatttgata atttttttca gttggtttaa tgatcccgct tatccctaat
    17461 tgcttgattc gctgttcctc gaccatggtg ggatccacta cctctacccg taggcgtgta
    17521 ttacaactat agacatccag gatattggct tttcctccaa acgcttgtag catttttgtt
    17581 gccatatatt cataattttt acttgctaac cctaactctg tttcttctaa ggttacttca
    17641 ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa tgtttctctt attgatcaaa
    17701 taataaaaag taacgtaata caaggcaaaa aagaaaattc cgattggaag aatcaaaata
    17761 gcattggtag cgattcgaaa attgatgaga taatcaacaa tacatgctcc gaatgtaaaa
    17821 ccaattcgta tattgaataa gtaacagaca atccctgcta aaccggcaaa aagtgcatgg
    17881 atcacgtaca acataggaga aagaaacatg aaactaaatt caacaggctc agtgatattt
    17941 gcaataaatg aagttcctgc accgcttccc atgattcctt tgaccatctc tttattttct
    18001 ttataagctg ctttatagat agctagacat atagctggta taccaaacat cattactaca
    18061 aaaaaaactg ataaaaacag tccagcctga ggatctccgt tgataaagcg tgtcatctct
    18121 cctctgaaaa cttccttgct aggagatgta tagcttccta aatcgtaata aatatacgta
    18181 ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat tcaaaaacat aaatatcccc
    18241 acacctaatg ctcccatatc aacgagcagt tttgcaaaat tatcaatccc aatttggatg
    18301 agcggccaga taaagcccaa aaaaacagct gcgattgttt gtagaatcat caccatagtc
    18361 aatgggaatt tttcgcccgc aaaataagaa aaaactactg gtaatttttg ttctttaaag
    18421 cgattatacg tccatgccgc tactaaaccg gaaataattc caccaaaaat ccccatattc
    18481 acttctgggt ccataatttc taagcctttt ttcaaggtag caatcgctaa aaaaccagta
    18541 agtgcaggta ttcctttgtc cttatttttg acaaaaccga ttgcaactcc tatagcaaat
    18601 aagatatcca agtttccaaa aacggcattt ccagctgcac caattactgg gatgttcagc
    18661 atatcgtcag ctgataaacg catgatcaat cccgctaatg gcatcgctgc aactggaagt
    18721 agcatcgctc ttccaagctt ctgcacattt gatttaacat tcatttttat tcctcctcta
    18781 ttaaatatcc tcatatgtta ctaactcaat caaaggatca tttttcactt tttgagcaaa
    18841 tgctgaatga attaaaaatt cgacttggtg ggtacgagga tacacaaaac ttgaaccatt
    18901 taacaattcc tgatccaaat acgctgcgtg acacatgact tcgatcgttt tatcttctct
    18961 caatatcatg agtaaacttg ccaaatattc ttccacactc gtattgattt gtcgtttttt
    19021 ttcatcagtt tctcctacga catcgaaaca aggctcaaaa taatcgacat gacgaacttc
    19081 atcttttcgt tcaaagttgc tccgaaccgg caaatcatat ttttttgcta atgtaacaac
    19141 tacctcctga ttttcatcca aagtatgagt atgatgatga ctgtctaaat gagttggtat
    19201 gatccccgca tgataaattt tttgaatctg agcattccat tcctgataca gttgatcttt
    19261 gtcaatgtta aatggatgct gataataaga taaggatctg aactctccgc tttcgataag
    19321 agtatcgaca gttttgagga gtggtttgcc acacgtcaat gttaaatgga ctcctacacc
    19381 tagcctaggc atttcctttc ttagtttcac agcatgctcg aaacccggca tatttgccat
    19441 caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc ataatcccat aattcacacc
    19501 atggctatag ccaaaatcat ccgaattaaa aatcactttt cccatttcat tctccttctt
    19561 ttttctacta aaataggcgc aaccaaataa tcatagtcca tttactatgt gtcatttggt
    19621 tgcgcctata gatagttacg cccttatttt aatagattct tgctagatga attgccaaat
    19681 agatgatttc attttcttga atggcaagtt gaaattgctg ttcgatgata tctttaatct
    19741 ttagtgctgt acaatacgct ttgctatgtt taaatgtttc agtaaaaaaa ccaatatttt
    19801 ctactggcgt ttcttcctga taaatcaagc gtttaaccag gaatttgata tgaaccatta
    19861 agcgatgata agtcagtgat tctttatcat aaacgtttga ataggatttt tctagatggg
    19921 acaaaatcgt ggtcgtcatt tctactattt ttaagctctt taaactatta ggctctccta
    19981 gctcagcatt aatgatatgc atagtgatga atcctacttc atttgaccct aactcggttt
    20041 gatatcgatg attgattcgc tcgaccactt ctactgctat atcatattca tttggataaa
    20101 ggaatctcat ttctggcaac ataattgctg tgacatctct atcttctctt tttcgttgaa
    20161 tagcaaaata aatatgatct gtcaatgtaa tatagatatt tgaatctaat ggagaggaga
    20221 tcctagctgt tatcagttca atcacttctt ccgcaaaatc cataaattcg attggaatct
    20281 gcgagactat ctcactgaat cgtttgatct tttctttaga atctaataca aataatttgt
    20341 cagcatcctc aacatcaaat ggcttactca ctttagcaga aaagccaatc cctttcccca
    20401 tcgcaatttt ttctattcca tcgtcatcga tcaatacagc attatgattc agctttttca
    20461 tcacatacac cgttaattca cctcctctat atgataaaac aaaacaactt gcagactcct
    20521 tcatacatat ttttgtctcc aaacaatttt taaagacaaa aaacgaccta agaaagaacc
    20581 ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca taatttacaa aaatagttta
    20641 acacataata aaagcgttat caatatcttt tttactcgtt ttatgataat acttgtatcg
    20701 aattgatttg gaggtaatta cataaaaaca aaaaaaatat agcaactctc tataagaaag
    20761 ttgctatatt gataattata ccggcggccg gggtcgaacc ggcacgtcct caacggacac
    20821 tggattttga gtccagcgcg tctgccaatt ccgccacgcc ggcaaattta aaaataataa
    20881 ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt
    20941 ggctataccc caagaataaa gggcgaatga tgggaatcga acccacgaat gccggagcca
    21001 caatccggtg cgttaaccac ttcgccacat tcgccatcat aaaagatgtt atttaatttc
    21061 tcaataaaaa actaagatga agacatctta gtcaataccg gcggccgggg tcgaaccggc
    21121 acgccctcaa cgggcactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc
    21181 aagttgattt attagtacta aggcggtaac cggatttgaa ccggtgatga aggttttgca
    21241 gacctctgcc ttaccacttg gctataccgc cattatacta ttatcaatct ataaaataaa
    21301 gaataaatta tagaaactgg ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc
    21361 ccttgcctta ccgcttggct ataccccaag aataaaaagg gcgaatgatg ggaatcgaac
    21421 ccacgaatgc cggagccaca atccggtgcg ttaaccactt cgccacattc gccatggcag
    21481 gggcagcagg aattgaaccc acactaacgg ttttggagac cgctgttcta cctttaaact
    21541 atgcccctat aaaatggagg agagtggatt cgaaccactg aaccctaagg aacggattta
    21601 cagtccgtcg cgtttagcca cttcgctact cctccatggt ggcgcgggac agaatcgaac
    21661 tgccgacaca cggagcttca atccgttgct ctaccaactg agctaccgcg ccatatttta
    21721 aaattattaa ttgaataaca agaacgacgg ttccgacggg aatcgaaccc gcgatctcct
    21781 gcgtgacagg caggcatgtt aacccctaca ccacggaacc agttcattat ggaggttaac
    21841 gggatcgaac cgctgaccct ctgcttgtaa ggcagatgct ctcccagctg agctaaacct
    21901 ccaacattca attaaaatga cccgtacggg attcgaaccc gtgttaccgc cgtgaaaggg
    21961 cggtgtctta accgcttgac caacgggcca ggtaaatttg ctacggagag taagggattc
    22021 gaacccttga gacagtttgg caccatctac atgatttcca atcatgctcc ttcggcctct
    22081 cggacaactc tccagagtga aagaagctca atactctttc tcaaaatgag
//
LOCUS       DANKMN010000054        20207 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000054 DANKMN010000000
VERSION     DANKMN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..1835)
                     /locus_tag="PW224_001934"
     CDS             complement(<1..1835)
                     /locus_tag="PW224_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2908606.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="PW224_001935"
     CDS             complement(1832..2536)
                     /locus_tag="PW224_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2908607.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="PW224_001936"
     CDS             complement(2548..4857)
                     /locus_tag="PW224_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2908608.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="PW224_001937"
     CDS             complement(5052..5516)
                     /locus_tag="PW224_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908609.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="PW224_001938"
     CDS             complement(5516..6142)
                     /locus_tag="PW224_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2908610.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="PW224_001939"
     CDS             complement(6149..6514)
                     /locus_tag="PW224_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2908611.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="PW224_001940"
     CDS             complement(6504..6842)
                     /locus_tag="PW224_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908612.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="PW224_001941"
     CDS             complement(6832..7158)
                     /locus_tag="PW224_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2908613.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="PW224_001942"
     CDS             complement(7139..7417)
                     /locus_tag="PW224_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2908614.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="PW224_001943"
     CDS             complement(7419..8783)
                     /locus_tag="PW224_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2908615.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="PW224_001944"
     CDS             complement(8796..9365)
                     /locus_tag="PW224_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDL2908616.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="PW224_001945"
     CDS             complement(9337..10524)
                     /locus_tag="PW224_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2908617.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="PW224_001946"
     CDS             complement(10588..12315)
                     /locus_tag="PW224_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2908618.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="PW224_001947"
     CDS             complement(12312..12764)
                     /locus_tag="PW224_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL2908619.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="PW224_001948"
     CDS             complement(12876..13256)
                     /locus_tag="PW224_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2908620.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="PW224_001949"
     CDS             complement(13253..13639)
                     /locus_tag="PW224_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908621.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="PW224_001950"
     CDS             complement(13641..13919)
                     /locus_tag="PW224_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908622.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="PW224_001951"
     CDS             13967..14695
                     /locus_tag="PW224_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HDL2908623.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="PW224_001952"
     CDS             complement(14841..15317)
                     /locus_tag="PW224_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HDL2908624.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15624)
                     /locus_tag="PW224_001953"
     CDS             complement(15277..15624)
                     /locus_tag="PW224_001953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908625.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="PW224_001954"
     CDS             complement(15611..15796)
                     /locus_tag="PW224_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908626.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="PW224_001955"
     CDS             complement(15797..16069)
                     /locus_tag="PW224_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HDL2908627.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16269)
                     /locus_tag="PW224_001956"
     CDS             complement(16066..16269)
                     /locus_tag="PW224_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908628.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="PW224_001957"
     CDS             16394..16606
                     /locus_tag="PW224_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908629.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="PW224_001958"
     CDS             complement(16639..16833)
                     /locus_tag="PW224_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908630.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="PW224_001959"
     CDS             complement(16846..17028)
                     /locus_tag="PW224_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908631.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="PW224_001960"
     CDS             complement(17025..17165)
                     /locus_tag="PW224_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908632.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="PW224_001961"
     CDS             complement(17655..18020)
                     /locus_tag="PW224_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908633.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="PW224_001962"
     CDS             complement(18060..18362)
                     /locus_tag="PW224_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2908634.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="PW224_001963"
     CDS             complement(18362..18661)
                     /locus_tag="PW224_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908635.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="PW224_001964"
     CDS             complement(18654..18818)
                     /locus_tag="PW224_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908636.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="PW224_001965"
     CDS             complement(18824..19093)
                     /locus_tag="PW224_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908637.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="PW224_001966"
     CDS             complement(19105..19854)
                     /locus_tag="PW224_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908638.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..>20207)
                     /locus_tag="PW224_001967"
     CDS             complement(19851..>20207)
                     /locus_tag="PW224_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908639.1"
                     /translation="NVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCI
                     CGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGI
                     KLNQKTIKKLNIGGEK"
BASE COUNT         5603 a         4273 c         3141 g         7190 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacatt
//
LOCUS       DANKMN010000055        20183 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000055 DANKMN010000000
VERSION     DANKMN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="PW224_001968"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="PW224_001968"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL2908640.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="PW224_001969"
     CDS             1013..2260
                     /locus_tag="PW224_001969"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL2908641.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="PW224_001970"
     CDS             2336..2680
                     /locus_tag="PW224_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908642.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="PW224_001971"
     CDS             complement(2797..3123)
                     /locus_tag="PW224_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908643.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="PW224_001972"
     CDS             complement(3496..4698)
                     /locus_tag="PW224_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2908644.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="PW224_001973"
     CDS             4815..5330
                     /locus_tag="PW224_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2908645.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="PW224_001974"
     CDS             complement(5529..5975)
                     /locus_tag="PW224_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2908646.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="PW224_001975"
     CDS             complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="PW224_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2908647.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="PW224_001976"
     CDS             complement(7854..8483)
                     /locus_tag="PW224_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2908648.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8467..8931)
                     /locus_tag="PW224_001977"
     CDS             complement(8467..8931)
                     /locus_tag="PW224_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2908649.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="PW224_001978"
     CDS             complement(9418..10686)
                     /locus_tag="PW224_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2908650.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="PW224_001979"
     CDS             complement(10917..11654)
                     /locus_tag="PW224_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2908651.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="PW224_001980"
     CDS             complement(11721..12290)
                     /locus_tag="PW224_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL2908652.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="PW224_001981"
     CDS             12418..13290
                     /locus_tag="PW224_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2908653.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13632..14504
                     /locus_tag="PW224_001982"
     CDS             13632..14504
                     /locus_tag="PW224_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2908654.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14501..16459
                     /locus_tag="PW224_001983"
     CDS             14501..16459
                     /locus_tag="PW224_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2908655.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16710..17561)
                     /locus_tag="PW224_001984"
     CDS             complement(16710..17561)
                     /locus_tag="PW224_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2908656.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17684..18151
                     /locus_tag="PW224_001985"
     CDS             17684..18151
                     /locus_tag="PW224_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL2908657.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18414..18896)
                     /locus_tag="PW224_001986"
     CDS             complement(18414..18896)
                     /locus_tag="PW224_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL2908658.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18996..19859)
                     /locus_tag="PW224_001987"
     CDS             complement(18996..19859)
                     /locus_tag="PW224_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2908659.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6068 a         4157 c         3662 g         6296 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tacaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgactcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactaac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13441 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13501 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13561 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13621 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13681 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13741 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    13801 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    13861 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    13921 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    13981 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14041 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14101 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14161 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14221 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14281 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14341 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14401 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14461 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14521 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14581 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14641 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14701 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    14761 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    14821 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    14881 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    14941 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15001 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15061 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15121 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15181 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15241 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15301 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15361 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15421 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15481 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15541 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15601 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15661 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15721 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    15781 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    15841 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    15901 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    15961 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16021 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16081 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16141 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16201 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16261 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16321 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16381 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16441 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16501 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16561 caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac
    16621 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16681 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16741 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    16801 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    16861 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    16921 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    16981 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17041 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17101 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17161 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17221 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17281 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17341 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17401 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17461 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17521 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17581 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17641 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17701 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    17761 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    17821 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    17881 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    17941 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18001 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18061 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18121 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18181 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18241 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18301 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18361 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18421 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18481 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18541 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca
    18601 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18661 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18721 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    18781 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    18841 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    18901 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    18961 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19021 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19081 gcttcgacta acccataatc tgaagaagga agccattctg catagatttt cccccaagta
    19141 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19201 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19261 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19321 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19381 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19441 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19501 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    19561 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19621 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19681 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19741 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    19801 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    19861 tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    19921 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    19981 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20041 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20101 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20161 gaacgctgct tggatttttc ttt
//
LOCUS       DANKMN010000056        20171 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000056 DANKMN010000000
VERSION     DANKMN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            275..1753
                     /locus_tag="PW224_001988"
     CDS             275..1753
                     /locus_tag="PW224_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908660.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2030..4090
                     /locus_tag="PW224_001989"
     CDS             2030..4090
                     /locus_tag="PW224_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL2908661.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4173..4742
                     /locus_tag="PW224_001990"
     CDS             4173..4742
                     /locus_tag="PW224_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2908662.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4917..5723)
                     /locus_tag="PW224_001991"
     CDS             complement(4917..5723)
                     /locus_tag="PW224_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908663.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5861..7375
                     /locus_tag="PW224_001992"
     CDS             5861..7375
                     /locus_tag="PW224_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL2908664.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7778..8761)
                     /locus_tag="PW224_001993"
     CDS             complement(7778..8761)
                     /locus_tag="PW224_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL2908665.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8766..9413)
                     /locus_tag="PW224_001994"
     CDS             complement(8766..9413)
                     /locus_tag="PW224_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2908666.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9498..10130)
                     /locus_tag="PW224_001995"
     CDS             complement(9498..10130)
                     /locus_tag="PW224_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2908667.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10146..10697)
                     /gene="coaC"
                     /locus_tag="PW224_001996"
     CDS             complement(10146..10697)
                     /gene="coaC"
                     /locus_tag="PW224_001996"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL2908668.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10694..11431)
                     /locus_tag="PW224_001997"
     CDS             complement(10694..11431)
                     /locus_tag="PW224_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL2908669.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11428..12417)
                     /locus_tag="PW224_001998"
     CDS             complement(11428..12417)
                     /locus_tag="PW224_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2908670.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12675..13217
                     /locus_tag="PW224_001999"
     CDS             12675..13217
                     /locus_tag="PW224_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2908671.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13234..14319
                     /locus_tag="PW224_002000"
     CDS             13234..14319
                     /locus_tag="PW224_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2908672.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14331..15137
                     /locus_tag="PW224_002001"
     CDS             14331..15137
                     /locus_tag="PW224_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908673.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15138..15965
                     /locus_tag="PW224_002002"
     CDS             15138..15965
                     /locus_tag="PW224_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908674.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15962..17035
                     /locus_tag="PW224_002003"
     CDS             15962..17035
                     /locus_tag="PW224_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2908675.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17463..20159
                     /locus_tag="PW224_002004"
     CDS             17463..20159
                     /locus_tag="PW224_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2908676.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         6603 a         3495 c         4060 g         6013 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactagatat aactagatat aactagatat attacttaac
     1861 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
     1921 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
     1981 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
     2041 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
     2101 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
     2161 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
     2221 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
     2281 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
     2341 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
     2401 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
     2461 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
     2521 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
     2581 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
     2641 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
     2701 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
     2761 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
     2821 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     2881 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     2941 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     3001 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     3061 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     3121 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     3181 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     3241 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     3301 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     3361 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     3421 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     3481 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     3541 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     3601 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     3661 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     3721 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     3781 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     3841 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     3901 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     3961 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     4021 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     4081 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     4141 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     4201 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     4261 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     4321 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     4381 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     4441 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     4501 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     4561 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     4621 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     4681 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     4741 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     4801 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     4861 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     4921 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     4981 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     5041 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     5101 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     5161 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     5221 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     5281 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     5341 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     5401 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     5461 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     5521 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     5581 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     5641 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     5701 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     5761 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     5821 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     5881 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     5941 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     6001 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     6061 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     6121 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     6181 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     6241 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     6301 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     6361 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     6421 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     6481 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     6541 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     6601 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     6661 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     6721 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     6781 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     6841 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     6901 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     6961 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     7021 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     7081 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     7141 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     7201 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     7261 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     7321 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     7381 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     7441 ttgaacactg tttagtgaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     7501 gccaagtaaa aacagcttct tatgttttta acttggttgg acttattacc gcagatgcaa
     7561 gcttttgaac accgtttatt cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt
     7621 ctcacgctct aaacacgtat aaacggcgga agcagaagaa attccttcgg aaataagctg
     7681 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     7741 cgcttctgtc ccagcctctt ttttgatcag catgatttta ttcagttatc ttcaaaatga
     7801 tttttccgat gtttttgttt gcttccatat attcatgcgc ttgtttgact gcgctgaatt
     7861 ggaatgtacg gtcaatgatt ggccgaatct tgccattttc tagaaaaggc ccagcttttt
     7921 gcataaaagt ttgtgcgagc tctgctttat actgatcact tctaggtgtc aataatgtac
     7981 caagcagttg gacacgtttc ccaatcaata caccaaggtc tatctcaggc acgatggtcc
     8041 cacctagcat accaatcaag acccaacgtc catcaacggc tatagaagct aaattctttt
     8101 cccagtaaga agcgccgatg aaatccaaaa taacatcgac tccccgacct tcagtgattt
     8161 ttcgtacctc ttcttgaaag tcttgttttt tatagttgat caattcatct gcaccaagtt
     8221 ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa
     8281 gctgtctagc taattggatc gctgctgttc ccacaccact tgctcctgca tggatcaaga
     8341 cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa
     8401 cttctgagat tccagcagca gaaacatagt ctagcgtatc agggagaagg attgcacgat
     8461 cggcaggcat cacagcatat tcggcataac cacctagatt gactaatccg tatacttttg
     8521 tcccaggagt aaaagtggag atatcacttt tattctcaac tacctctcca gctacttcga
     8581 ctcccaaaat agggtaggga ggtttcaaag agactgattc tcttttcata atatctgttc
     8641 gattgacagc tgctgtatgg actttgatca gtaattcgcc ttgttttggt ttaggaacag
     8701 gcacttcaat ttgttctaat acatctacag aaccaggatg cttgattgaa atggcacgca
     8761 tggatctatt cctcctttgc ccattcttgg aatgctgtat tcaactcttt gctaccttca
     8821 gaaagtacta gagtcgtacg agaaatatcc gtttcttcca ctagagcggc aagtacagca
     8881 gcaactgtag agcgagtgat cgtttttgat gccttctcat taggcttagt taattgaatg
     8941 gaagtaactt cttcatcgtt agtcaatgtg actggttgaa gaatgacaaa atctaagtta
     9001 gttcgtttca gccattcatc tgcataatgt ttagtaatat aataatctgt catggactca
     9061 ggccattttg ctggttcatc tgcataaaca gcactgacca tcagatagcg agagacatgc
     9121 gcattttcag ctgcgatcac tgttttgatt gcgccatcca agtctacttg cagaagattc
     9181 tttccttgag agccggcagc aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt
     9241 ttttccacgg accaagttaa atcaaaagag acatacgaaa cattctcttt ttttactaat
     9301 gtttgggaag caacatctct cacccctgca aaaatttcat gggaacttgg agctaaatct
     9361 tttatcaggt gtcggccaat ttgtccgttt gcacctacaa caaaaatttt catttggatc
     9421 atcctttcga cgtcaaatct ttccattcaa tagtaacacc aaaagagaaa aaaatcagag
     9481 atcggctacg atgactatca gttagtaaga gaagggtgct tgaagtatct taacaagaca
     9541 ttgcatactg ctgcagtcgc aagacctgcg acgattcctt caggtatacc acttgtaaaa
     9601 ataaccgcta gaatagctga ataaacagca tttaaatttg ttccgatgac tttggcataa
     9661 tcttgctgga ataaaaagta gatcatattc atcactaaaa tcgtattggt caatgaaccg
     9721 gaaagtccag ctaagaataa agaaacggaa tctgcttttt gtttcattag tttgcgcaga
     9781 cctttataaa cgaaataagg aaccacccca atcaagatgc gaggaataaa cgcgatcagc
     9841 aaggctttcc agcttccatg ttctgttccg atgactggga taaacggaga aaagacaaaa
     9901 gacaaaggtg tttggataac tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca
     9961 agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa tgattacagg aatatgcatg
    10021 gttgttgcat tgattggacc gatagggata aaacctaaag gcgtaacagc taataaaatc
    10081 aaaatagcta aaaacatcgc agttaatgta aagttttttg tatttttcat aagtactcct
    10141 tttttctaag catttctttc attaagagtc agcatgacct gttcgatgat cgtctgaata
    10201 tcagctaagg caccttttcc aaaatctcca caagctaaaa gagattctct tggttcaatt
    10261 tcctgatagc cgacagatct caatgtttcc agattacgct gagtgatcgg gttttgatac
    10321 atattcgtgt tcatggcagg ggcaatcaat tttggtgttc ccactggaag cgccattgcg
    10381 actgttgaaa gaagatcgtc tgcaatgccg tttgctagct tgccgataat gtttgctgta
    10441 gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt cgatatgatt gattacctgc
    10501 ggatttgatt cttgcatgac gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt
    10561 aatggcgtga taaaatgtgt gctgctttta gacataatca catctacctg ggcacctaat
    10621 ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag aaatacttcc agtaacgcct
    10681 agtaagatct ttttcatttt gattcctcca gtctaatttt ctcagtaatc aatgcagcaa
    10741 tctcagattt actttgagct tcttccactg ttccatcttt agataagaga tagccgtggt
    10801 gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta
    10861 tcagactatt ttttgcgact gccagaagag attcctttgg tacatctact aatagtttga
    10921 aaccaatgat ttttgctttt ggctgccatt cacgtagata agaaatgatt tttggtgttt
    10981 tcttcaaaac gagcacaaga tgctcggtgt tagaagagat ctttgaaaca gtggtatctg
    11041 tctgttcatt ttccgcaaac caattgtcca gttcttcttg cgttgaagaa gtgggtaggt
    11101 tttttgccag ctgttcttct gaaaaactaa aagcaggagt aaaatcactg actgccatac
    11161 tgtggataat cgcatcgtag tgttcttttt tcgttagttc ttctaattgt aaaaataagt
    11221 ccagtgcaga ttcgatcgta taaatcgtta tattggagtg tcttttcggt ttcagtgctt
    11281 gtttcgtagt cacgtaatcg attgtggtat ccggattggc agctaaataa tcagctaatg
    11341 cttgccctaa tcgtcctgtc gaatgatttg taatagcacg tacttgatcg attttttcag
    11401 tcgttcctcc agctgtgatt agaatcttca tttatggtca cctacattta ctagtgttct
    11461 tccttggtgt gttccagcaa gtagtttaga tgctgtttca ggcaattgat ccagagtaat
    11521 ctcttggacg accagttcat ctgcaatctg caaatccgcc atctgttgcc ataaagaaag
    11581 acgttgatct attggtacat tgacagaatc aattccaatc aactggatgt ttcgcagaat
    11641 aaaaggcaag acagtcgcgt ttagtttgat tcccccagca ttgccacata aagctactgc
    11701 accgttataa gagatcaatg gaagaagact gcttagctgc tcgccaccta ctgtgtcgat
    11761 gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc tctggcagaa attcatcagg
    11821 tcgtacgact tgcaaagcac ctagactttt tagccatgtg acagactctt cttttctgga
    11881 aaaggtaatg attgactgga atcctagttt atgtaacagg gctatcgctg tactgcctac
    11941 accgccagaa gcaccagtaa cgacaacttt tttatcggag gtcatacctt gacggattaa
    12001 agcgttcaca cacaacatag cagtaaaccc tgctgtacca aggatcatcg cttgacgcag
    12061 cgtcatattt tttggtagag gaactaacca gtctcctggt actttttgat attggctata
    12121 accgcctgga tgggtgacgc caagcccaaa accagtagca agtacaagat cttcttttga
    12181 aaaacgtgga ttgttacttt ggacgacagt tccggataaa tcaatccctg gagtcatcgg
    12241 atatgttcgt atcacctgac cagactcgct cattgccaat gcatctttat aattgacatc
    12301 agaataggca atttttacca agacttcatt ttcagccaac tcagtaaatg gcagatcact
    12361 gattgctgta tggaaaacag gttcctttat tacctgaaaa gatcgaaaag cgttcattcg
    12421 tgagacactc cttttaaaat tttcttttat cataccataa agcctactct tttattcttg
    12481 ttcttccgta aatttagaca aaaattaggt gaaaaataaa gaatcaaaca actagaattt
    12541 attccaaaag ataaagaaat attcagcaaa aaaaatttta caaagggttg cctttttcaa
    12601 taaatttcgt atactaatac gagtggtagt aaacaggaag tttactatta gaaaacttcg
    12661 attcggagga aattatggaa attggtgaga agttaagaaa tttgcgcatc caaaagaatt
    12721 tgacacaaga agaactaggc gaacgcacag atttgtcaaa agggtatatt tcccagttgg
    12781 aacgagattt aagttcacca tcaatggaaa cattcttttc tattcttgaa gtcttaggtg
    12841 tgactccaga agaatttttt catcaagaag cagcgaatct gcaagtggtt tattcaaaag
    12901 aagatcatac ggtttatttc gatgaagaaa acgggtatga attggaatgg ctcgtggctg
    12961 attcgaatga aaaagaaatg gagcctgtct tgctgacttt tgaaggtgca ggagaatata
    13021 agacgtttga accttcatta tctgaaacgt ttatttttgt attagaaggg gaattagagt
    13081 taagcttagg agagacggtc tatgtggcaa aagcaggaca gtcgatctat tatcaagcga
    13141 caaaacaaca tcaattaagg aatcatctca aaaaacgaac aaaagtattg attgttgcaa
    13201 cgcaatcata tttgtaatat agggggtaaa aacgtgggga aaccaatcat ttcatttgat
    13261 caagtagtga aacgctatga tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa
    13321 caaggaaaat tttatacact gcttggtcct tccggctgtg gtaaaacaac gatcctacgt
    13381 attatagctg gattcaccga agctacagaa ggggacatct attttgaagg gaaacggttg
    13441 aatgatttgc cagctaataa gcgacaagta aatacagtct tccaagatta tgcacttttt
    13501 cctcatatga atgtttttga taacgttgct tttggattga agatcaagaa aatgtccaaa
    13561 tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa
    13621 aaacgggaga ttagtgaaat gtctggtggt caaagacagc gtgtagcgat tgcacgagca
    13681 attgtaaatg aaccgaaagt attgctatta gatgaaccgt tatcggcgct tgatttgaaa
    13741 ctacgtacag atatgcagta tgaactgcgg gatctgcaac aacgattagg aatcacgttt
    13801 atttttgtta ctcatgacca agaagaagcg ttggcaatga gtgatgagat ttttgtcatg
    13861 aataaaggga aaatcgtcca aagtggtaca cccgtagata tttatgatga gccaatcaat
    13921 catttcgttg ctgattttgt tggggagagc aatatcgtgg acggtgtaat gatcgaagat
    13981 aatctagttg agtttgttgg taagcaattt gaatgtgtcg atggcgggat gcgtctaaat
    14041 gaaccagttg aagttgtttt acgcccagag gatttgacga tcacgacacc agataaagga
    14101 aaactagtcg taacggtcga tactcagctg tttcgtggtg tccattatga aatcatctgt
    14161 tatgatgaac agcaaaatga atggatggtc cattcaacga aaaaagctaa agaaggaagc
    14221 aaagtaggat tggcctttga gccagaagat atccatgtca tgcgtttcaa cgagtctgaa
    14281 gaagaattcg atgcacgtct tgacagctac gaagaatagg ggggacaaga atgaaaacaa
    14341 tgcgcaggat ctattccgtc ccttatattt tgtggctcgg tttattcgtg atcgcgcctg
    14401 tattaatgat catttatcaa tctttttttg atatgaatgg gcaattcacg ctgaataatt
    14461 atctatctta ttttacgtcg gggacatcgc tttctatgac gatcaattct gtatggtatg
    14521 catttttgat cacgtttttc acgctgatca tcagttaccc gactgcttat tttttaacaa
    14581 aactgaagca caagcaatta tggctgatgc tgattatttt gcctacatgg gtgaatctgc
    14641 tgcttaaagc ttacgcattt attgggattt tcagtgtgca cggcagcgtg aatcatttct
    14701 ttgattttat cgggatcggg ccgaaacaga tattattcac agacttcagt tttctgttcg
    14761 tggcaacgta tatcgagatt ccatttatga ttatgccaat tttcaatgca ttggaagaga
    14821 tgaatccttc tttgatcagt gctagtagag atttaggcgc gaatagcttt gagaccttcc
    14881 gacgagtgat ttttccgtta tcattaaatg gtgtgaaaag tggtgtccaa gcagtattta
    14941 ttccttctct ttctttattc atgctgactc gtctgattgg cggaaatcgg gtgatcactt
    15001 taggaacggc tatcgaacag catttcttgg ttacacaaaa ctgggggatg ggttctacaa
    15061 tcggtgtgat tttaatcatt gctatgttca tcgtgatgct cctgacaggc gagaagaaaa
    15121 aaggaggtcg ctcataaatg aaaaaattta aatggtcgta tctttattta gggatcgtct
    15181 ttttattctt atatgcaccg atcttctatc tgatttttta ttccttcaat gaaggaggaa
    15241 cgatgaacag ctttacagga tttacttggg aaaattatga agctgttttt gaagatacac
    15301 gtttgatcat tatcgtattg aatacgttca tgctggcttt cttatctgca ttgatcgcca
    15361 cgatcatcgg gacatttggg gctatgggaa tctattacac gaaaaaacga cgtgcacgga
    15421 cagcgttgtt gagcatgaat aacatcttat tagtctctcc tgatgttatt atcggtgcta
    15481 gtttcttgat tttctttaca ctactaggat tcggattagg ttttcagagt gtattgcttt
    15541 cccatattgc tttcagcatt ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa
    15601 tgaacgattc aatggtcgat gcggctagag accttggtgc taataatttc caagtagtca
    15661 aaaatatcat tttgccttat ctttctccag gaatcatagc tggatatttt atggcattta
    15721 cctattctct tgatgatttt gcggttacat ttttcgtcac aggaaatggc tttacgacgc
    15781 tctcagtaga gatctattct cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa
    15841 gtgcgttagt tttcttgttc tccatgatat tggtcattgg atactacttt attagtaaag
    15901 aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg cgagggggtg gcacggctgc
    15961 gatgaaaaaa ttacaatctc tgtttttagg tattctcatc attattgcgg tgctgctgct
    16021 tggtgttcgt caacttgaaa aaacaagtgg catgagtgga gcaaaggtac ttaccatcta
    16081 taactgggga gattatatcg atcctagttt gatcacgaaa tttgaaaaag aatatggata
    16141 caaagtgaat tatgaaacat ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg
    16201 tgggacaaac tacgacatcg ctataccaag tgaatatatg atccaaaaaa tgatcaaaga
    16261 aaaactagta cttccattag atcattcgaa gatcaaagga ttaaataata ttgatccacg
    16321 atttttagac ttggattttg atccagataa tcagtattcg attccatatt tttggggaac
    16381 tttagggatc gtatataacg ataaattctt tgatgcaaat caaattaagc attgggatga
    16441 tttgtggaag ccggaattaa aagacagttt gatgctgatt gacggtgcac gagaagttat
    16501 ggggctttct ttgaatagtt taggctattc tttgaatagt aagaatatgg cacagctgac
    16561 agaagcagca aataaattga atcatttaac accaaatgtc aaagcaatcg tggcagatga
    16621 gatcaaaatg tatatgatca atgaagaagc ctccgtggca gtgacatttt ctggcgaagc
    16681 tgccgatatg atgtgggaaa atgaacattt acattatgtg atcccaccag aaggatccaa
    16741 tctttggttt gataatatcg ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga
    16801 tttcatcaac tttatgctag aaccggaaaa tgcggcacaa aatgcagagt atattggtta
    16861 ttctacacca aatgaagaag caaaagcgtt attgccaaaa gacatttcgt cggacgaaca
    16921 attctatcca tctgatgaca cgatctccca tcttgaagtc tacaaagatc taggatcgaa
    16981 atacctaggc atctataatg acctattctt agaattcaag atgtatagga gataatggca
    17041 aaactcagta agaatgggga cttatcaacg ttctaaagag ctaaaaatcg tcctttttct
    17101 atctgagttt tccaatcttc taccaaaagc ggatgaaacg ccgctttttt tgtgttattt
    17161 tattttttct accacgtttt tctaccagag aaaagtggac aaaaccattg taaaataggg
    17221 cccgagacgg gctagtaaat ctaagattga aaaagttaca gaaggataaa gtaattggaa
    17281 actatgcaaa tatacttaac ccttctgtaa tctattaata aaactaatat atgaatccag
    17341 ttcaatattt gtagctatga aaaaacataa aaatcatatt agataaaaac taaattggta
    17401 gataaattat tttgtcagat atctgaggca aatattcgac tatttgtgtt tcaaagtaaa
    17461 aattggaata caaatataga atagacatat tgcagccgat agtttattat gcttttagac
    17521 acattaaact ggaggaaaaa atgaaaaaga gcgctaaaga tcgagttttt gatgagctga
    17581 tacgactaac agggaatatg tcttctgtca ctgccgagaa aatttcgaag aatctaggta
    17641 tctctcgtca gaatgccagc cattatttga cgagattagt ggaagataaa aaggtcgaga
    17701 aattaccggg aaagcctgtt ttgtggaagc ctttagatga atatgcggtt gtagataata
    17761 cggagcaaat aaacgaagcc tttcattctg tagttggtca tgacggaagt ctgagagagg
    17821 tcattcaaaa atgtatagcc gctgtcaaat atccgcctaa cggactcagt gtattaataa
    17881 atggagccac gggtgttgga aaaagttttt tagccacaaa aatttttgaa tacgctatcc
    17941 atgagcaaat tattgaaaaa gatgcaccat ttgctatctt aaactgtgcc gattatgcgg
    18001 acaatcccga actgctttcg gcaacactgt ttggatacaa gaagggggcc tttacggggg
    18061 ctgaaaagga taccgatggt ttattggcaa cggccaacaa cggctatctt tttctcgatg
    18121 aggtccatcg cctatcgaaa gaaaatcaag aaaagctctt tctatttatt gatactggaa
    18181 actatcgtcc agtcggtgaa aatgtaaatt ggcattctgc taaggttcgt tttattttcg
    18241 ccacgacgga aaaaggtgaa aattatttat tagacacctt tgatcggcgt attcaaatta
    18301 gtgttatgtt gccaactttt gaagatcgcc ccattcgaga acgattagag ctgattcaat
    18361 tattttttca aaatgaagcg gatgttttac aaaaagatat tattgtttca agagaagcct
    18421 tgtctttaat gttatctcat ccctttagtg ggaatattgg taagctgaag aatatcatta
    18481 aaatcagttg tgcggatgtg tatagtcgaa cacaagaaga accattaacg attgggaaaa
    18541 atgagatctt gattcagtta aatatgctgg aatccgaagt agaaagcttg ccgattgcta
    18601 gtttattgat aaagaaaaat caaaagataa aaacggatcg aatcaaaccg tttgattatg
    18661 attcgatttt ggaaaaaatt actcagctga taaatgaagg taatttcaat gaaaattttt
    18721 cttcgataaa gaatgaagta cagcatttgg tgcatcacat tgataaggat tcttttttat
    18781 caatgagtag aaaattgatg attcagatgt ttagaaaggt ctgggtatcc gttttaggaa
    18841 aaacgtacgg attgacgacg agtggacctg ttgcagaaat tattagcaga tcctatgtca
    18901 tttccccaag ctataagaca gacttaaccg ggataaatcg tacattagct gcaaatctac
    18961 ctcggacgac ttacttatgt aatcagttct tggagcattt accaccgctc tcagaaaagg
    19021 acgagaactt tctaagactg cttattgtcg tgactttatc tgattatgta gatgaatcca
    19081 tcgagctgaa gggcctgttg cttgctcacg gagatagtac ggctagcagc attcaagcag
    19141 tagtgaatca attatgcggc aattatgttt ttgaagcgtt ggatatgcca gtagagagta
    19201 atgtgtcaga gattgtcgaa tcgacaagga atttcgtgaa acggcaagtg aatacagata
    19261 gtctagtgct gatcgtagat atgggctctt taaatcaaat ttacacacag atcaagaacg
    19321 atttaaaagg ggagttactt cttctgaata atttgacgac ttccattgca cttgatattg
    19381 gcttgaagat gacgatgaat gcgccattta aacagattgc ggaggatgca gcagacaagt
    19441 attcaatcaa tgttcaatac tttgaaggct tttctcaaag tagcaacatt atcatttcct
    19501 gtatgtcggg tttggggatt tcggacaaat tgaaagatgt ttttcgtcat catttatcgg
    19561 atgaatccat cgaagttttc acgaaggact acagagaact acgtgaatta atcgaccaaa
    19621 atgatgaaac ttattttgac aagacaaaat tggtcatcac gacttcggat cttccacgtt
    19681 ccttttcaat tcctaatatt aatgtttatg acatcttaga cggtgatgga gcagccaatt
    19741 tggagcatat tttgagtcct gaaatgaagc cggcagaatt taaccgcttg attcaggagc
    19801 tagtaaaatt cttttcgttg gaaggaattg cggaccgttt gaactttctg aacccaacga
    19861 tcgtgatgag tgaagtggaa acagtcattt ctaaatatga aaattactat catttcactt
    19921 taaatggccg tgtaaaacta aatctctata tgcatacggc attgatgatg gaacgtttat
    19981 tcttggctcg gacagaaaaa tctgaagaac cgaattatga tttgaaacca gatgaagatg
    20041 aattttatta tgtttctaga agtatttttc aatcaatgga gatgaaatat aattttaaag
    20101 tcaatgctta tgagctttcg ctgctctatg aattattagg accgtatatt caaaaataaa
    20161 gtgtctaaaa a
//
LOCUS       DANKMN010000057        19502 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000057 DANKMN010000000
VERSION     DANKMN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(455..1084)
                     /gene="udk"
                     /locus_tag="PW224_002005"
     CDS             complement(455..1084)
                     /gene="udk"
                     /locus_tag="PW224_002005"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL2908677.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1168..1863)
                     /locus_tag="PW224_002006"
     CDS             complement(1168..1863)
                     /locus_tag="PW224_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL2908678.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1876..2592)
                     /locus_tag="PW224_002007"
     CDS             complement(1876..2592)
                     /locus_tag="PW224_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL2908679.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2922..3527
                     /locus_tag="PW224_002008"
     CDS             2922..3527
                     /locus_tag="PW224_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL2908680.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3852..4175
                     /locus_tag="PW224_002009"
     CDS             3852..4175
                     /locus_tag="PW224_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2908681.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4357..5727
                     /locus_tag="PW224_002010"
     CDS             4357..5727
                     /locus_tag="PW224_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL2908682.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5857..7158)
                     /locus_tag="PW224_002011"
     CDS             complement(5857..7158)
                     /locus_tag="PW224_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2908683.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7388..9346)
                     /locus_tag="PW224_002012"
     CDS             complement(7388..9346)
                     /locus_tag="PW224_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL2908684.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9523..9789)
                     /locus_tag="PW224_002013"
     CDS             complement(9523..9789)
                     /locus_tag="PW224_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2908685.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10299..12425)
                     /gene="pnp"
                     /locus_tag="PW224_002014"
     CDS             complement(10299..12425)
                     /gene="pnp"
                     /locus_tag="PW224_002014"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL2908686.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12613..12882)
                     /gene="rpsO"
                     /locus_tag="PW224_002015"
     CDS             complement(12613..12882)
                     /gene="rpsO"
                     /locus_tag="PW224_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL2908687.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13133..13696
                     /locus_tag="PW224_002016"
     CDS             13133..13696
                     /locus_tag="PW224_002016"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2908688.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13949..14470)
                     /locus_tag="PW224_002017"
     CDS             complement(13949..14470)
                     /locus_tag="PW224_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL2908689.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14473..15315)
                     /locus_tag="PW224_002018"
     CDS             complement(14473..15315)
                     /locus_tag="PW224_002018"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL2908690.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15308..16033)
                     /locus_tag="PW224_002019"
     CDS             complement(15308..16033)
                     /locus_tag="PW224_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL2908691.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16209..16505
                     /locus_tag="PW224_002020"
     CDS             16209..16505
                     /locus_tag="PW224_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908692.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17073..18665
                     /locus_tag="PW224_002021"
     CDS             17073..18665
                     /locus_tag="PW224_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2908693.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18678..19418
                     /locus_tag="PW224_002022"
     CDS             18678..19418
                     /locus_tag="PW224_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2908694.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4002 c         3697 g         6223 t
ORIGIN      
        1 tgcgacaaaa gagcttcttc aaaaaaataa gtcgaaatct cacacaaatt cgagaaataa
       61 ttttcgtaag tttcttctta tttattgcat ctgaccactt ctgttacaac ctctcgttaa
      121 acggtgttca aaagcttgca tctacgataa taagcccaac ccaacgaaaa acgtgaggag
      181 ctgttttatt tggatgctct tatcgcttga tgcaaagaca gcttttttac aacctctact
      241 tcacggtgct caatcagctg accttcggta ttagctcaaa aaacggtaaa atataaacag
      301 ctattttccg tttcttcttt ttaatactta ggtcaagatg ctgttttcac aacctcttgt
      361 tataaataaa aaaaacagtt ggctggtgac tgttcaaaaa gcagaatcct tgcttttcaa
      421 acggtcgcag tccgttcaac tgtttcgctg cgcactattt atggtttaag aaactagcta
      481 cttttgttgt aatcaaatca atagcaacgt ggttttctcc gccttctgga acaatgatat
      541 ccgcatagcg cttcgttggt tcgataaatt gatgatacat cggtttaaca actgtcaaat
      601 attgttcgat cacggagtcc aatgtgcgtc cccgttcttc catatcccgt ttgatacgac
      661 gaatgatacg aatgtcatca tctgtatcta cgtatacttt gatatccatc aagtcacgca
      721 gacgttcgtc ttctaaaatc aggattcctt ctagaatgat cacttctttt ggttcctgaa
      781 tgatcgttgc ctgacttctt gtatgcgcta catagtcata cacgggtttt tcaatggctt
      841 tatagttcag taagtcgcct acatgctgaa tcagcagatc attatcaaag gcaaatgggt
      901 gatcgtaatt tgtgttcaat cgttcttcaa aactcaaatg gctctgatcc ttgtaataag
      961 aatcttgttc aagcatcatg atggaatggt ccgggaagtt gttgaaaatc gcacggctga
     1021 cacttgtctt tccgcttccg gatccacccg tcacaccaat gataattggc ttgctttttg
     1081 tcattgacta aaacctcttt cgtatccgtt ttttcattct aatctattat agcgaaaaaa
     1141 cacgaaaatg ctatattttt ctgaaaatta atccttttca atttccaata atgaataaag
     1201 ttcctctaat gaatcgattc gataagtagg ctggatttct tcgatttctg gtaatttatt
     1261 tctattgaac caaatcgtat cgatccctgc catatttcct cctttgatat cagaagtcaa
     1321 agaatcacca atgatcacag tttgcttttt atcaaaatta ggaatgtggg aaaagacatg
     1381 atcaaaaaat tctttcatcg gcttttgcgc tccgacagct tctgagacaa agatatcctt
     1441 gaagtaatca agcattcctg catctgttaa tcgccgatgt tgcgtgtgcg acacaccgtt
     1501 cgtcacaata taaagatcag catgcggatg gacctttttc aggatttcta gacttcctgg
     1561 aacaagatca tgaccttggt tcaagtagtg gcggtagcgt ttttccattt gatcactatc
     1621 tactgttttc ccaaattgct ggaaaagtaa cccaaaacga ctgcctatta tttcttctcg
     1681 tgatagtctg cctttttcaa actcctgcca taaatgatga ttcaaagcct tatagctggt
     1741 ttcgatttct ggtgtcaatg tgattccttg ttctgcaaac aaagcactta aagcattggc
     1801 ttcagttaat tgaaaatcta acaatgtatc atctacatcg aacaataaag ttttatattt
     1861 catctttttg acctcctatt taaaataaat acttaacttt ttcatctctg ctaatgtcaa
     1921 cgatcgatac tcgcctactc ctagctgttc atctaattcc aatggtccca tgcttagccg
     1981 tttcaatgcg gtcactttct ttccttgggc taagaacatc tttttgactt gatggaattt
     2041 tccttcttgg atcgtcaata aaaccgtact ttggtgcttg tctgctttga gtatttttaa
     2101 atcagccggc ttacattgat acccatcagc gaatacgatt ccattttcaa atgctgttac
     2161 gtctgccggt gtcacccttt cgttcacaac tacttgataa gtctttgaaa cctttttttc
     2221 tggaagtagc atttcatatc ccaattgtcc attcgaagtg atcagcaata gtcctgttgt
     2281 atctctatct agtcttccca ctggatataa tggttggaaa cgatcggcac ttgcaatcag
     2341 atcgattact gtcgtctttt ctggatctga tacagctgta acagcttttt caggtttatt
     2401 gagaagataa taggactcat ttgtttctaa gtgttcacct gctacttcga tttgatgcag
     2461 taaactatct acatttcgat tttgtcgaaa ttcgactgtg ccatctatca tcacttgctt
     2521 caaagcaaac agccgcttca tttccttgcg tgtcgtttct aatttttctt ctattaattt
     2581 atctaaacgc atgctgtttc ctttcttatt actactaatt aattatacct tgaatattct
     2641 tgcttgcaaa tacatcatca tagttattcc ttgacttaat taacagttca cgttacggtt
     2701 aaatagtaaa gtgaattgga tttctcatac acagcgtaat gacctggtgg agaaaagttg
     2761 aatcctgact ttctaagggg agatgcgcgc atctctcctt ttgcgctttt ttaagcgccc
     2821 tgccttcgat gatgtgcacg agattagcct ttagaattca cttaaataaa atctgtccgt
     2881 taccaaagca gacagtcaca ttttaaggag gaattttggt tatgtcagta tcattagaag
     2941 taagtaaaag agaagtacgt ccccgttcat taagaaacaa acttcgtcac gaagggaaag
     3001 ttccagctgt tgtttatggg tataaagtag aaagtacccc tatctcattc gatggtttga
     3061 cactaagcaa attgttgcgt gaacatggag cgaacacagt catcacgatg gacatcgatg
     3121 gacaaaaagt caatacattg gttcacaaga tccaaacgaa cacttttacc aatcatttcg
     3181 aacatgcaga atttctatct gtaaacatgt cggaagaaac agaagtcgaa acagaagtcg
     3241 ttcttgttgg cgaatcagtg ggtgctaaat ctggtggagt gttagctcaa aacttgtata
     3301 cagtagtcgt atctgctaca ccagacaaat tgcctgaacg aattgaagtt gacgtgacaa
     3361 acttggaatt aggtgattct attacagtag ccgatcttcc aaaaaacgac gattacaaat
     3421 tcgtgaccga cagtgaagag caaatcgctg ctgtagtcgc tcaaagtgaa gaagaaagca
     3481 caggtgaagg tggcgaacca gaagtgatcg gttcaacaga agaataatac tgaaacaatc
     3541 aggtggtgtt agaagcggcg ggtcagcagc ttttaacacg ccttttttat ttctaatcgt
     3601 ttattttcat atggggcttc cacttttgta aatggcttta caaacgaaaa atcctaattt
     3661 accaagcttt tcctcgcata cttttatcgt acctttataa aaatttatac tttgaaaaaa
     3721 agtattcaat tattgttgca tttttcctga ttctttatta gaatgaaccc tcgttgaagt
     3781 aagtatttct agtttatcct aggattaatt ataaaaataa aacattcgtt gattcgaagg
     3841 aggttaaaca gatgtttgaa agttttgata gcagccgcaa ccgttacgct tcagttggtg
     3901 tcgtatcaag tttgccagat gaattgattg acagtatatg gtttattatt gacctagatc
     3961 ttaagggagt cattccatta gaaaatatat taacctttga tttgatcgaa aatcgtggaa
     4021 aagtgacgat gcatttttct caagcaggaa gcaatgtgga aatggatatt gatttgccgt
     4081 ttgcctattc gaaccaattt cctgctcaag tatacgccta tgatgacggt acgagagaaa
     4141 cgatcctttt accaagtgag attagacaat attaaggtga tagataaacg tggatgtccc
     4201 ttattttaca gaccaggcca agttgttgat tctttgactc aattcttggc ttctttttat
     4261 ggagtttcat aaattatcat tgatttctaa ttggtattct caggataaac gctatatact
     4321 actaactaaa ttttaaagat ataaggagtt taaaagatgc gcgatttaac aaaaggaagc
     4381 cccgctaaac tgattttgat gttcactgtt cctttactga tcggaaatgt ttttcagcag
     4441 ttctacaata tgatcgatat gatcatcgtg ggtcagacgc taggtaaaaa tgcgctggca
     4501 gcggttggtg caactggaag cctgacattt ttgatcattg gctttgccca aggtttaaca
     4561 gctggtttag ccattattac cgcacaacgt tacggtgcaa aagattatcg gggtctgaag
     4621 aaaagttttg ctgctagtgt tgtcattagt cttgtcgtca ctgtgatttt aactgtccta
     4681 agtctcctgt ttattcgtcc gatgcttcaa ttgatgcaaa ctcctcctga aatacttgat
     4741 caagcacaaa catttatttc aattatttta ttagggatct ttgcatcagt cagtttcaat
     4801 ttgctttcta atgtcattcg cgcacttggt gacagccgca caccactatt ctttctgatc
     4861 attgcggtca tcatcaacgt cgtacttgat ttgatcttta ttatctattt tggcatgggt
     4921 gtggaaggtg cagctattgc tacagtaatc gcacaagttt cttccagcgt actgtgtcta
     4981 gtgtatatca agaaaaaaat gcctttgtta cagttacgca aaaaagattt caaatttgaa
     5041 aaagaagagt ttgctgtaca cctaaatgct gctttgccaa tggcgtttca atcttcgatc
     5101 atcgctgttg gtgctatcgt gttacaagca gcactaaata gtctagggac agatgtcgta
     5161 gctgctcaag ccgctgctgg tcgtatcgac caatttgcaa atcagccgat gatgtcattt
     5221 ggtattgcaa tggcaacttt cactgcacaa aactatggtg cgaaagaata tggccgaatc
     5281 ttgcaagggg tacgtcaaac tttactgatg agtgtcggat ttagtctctt agcaggtgct
     5341 atcgtcattt tcttcggtca tttctttgtt caattgtttg ttagcccatc agaaacacgt
     5401 gtatttgaat tagcacaaac ctacttcaat atcaatgggt ctctttactg gatcttagca
     5461 gtcctgttca ttttacgcta cacacttcaa gggcttggac aaagcaagat ccctacgatc
     5521 gccggtatga tggaattgct tatgcgttct tttgcagcta tcgttcttac aggaatgtgg
     5581 ggatacccag gagcagcagc agcttctcct ttagcttgga tcggttctgt tgccgtgttg
     5641 ctttactctt atatgcgttc gatgaaacaa ttgaaacaca tgggtgaaga ggaacatttc
     5701 acattagaac atgcagaagc aagatagcct tgattaaaag aaatcgttgc ggctaaaaat
     5761 gaaaactttt attgaaaata tcttcttcgt ttaccgaaaa caggtttttt agataacaaa
     5821 aaggctgtga caaaaaatgt cacagccttt tttcttttat tctatttttc tttccgtgga
     5881 ctctactttg aaataatcga tatgtgcatg ctggtattct aataccgtga aacggaatga
     5941 tgcaatctca atgattgttc ctgcttttat ttctttatat cggctagaag caaagcccgc
     6001 aagtgtagta aaattcgttt cttcaaactc tggaatctct acatgaaaat aacgctggaa
     6061 atcatctaat gttagtttgc cggagatgat ataagagcca tctgactgtt ccgctatcaa
     6121 tgcttcgtcc aaatctgtct catctcggat ttccccgacg atttcttcta aaatatcttc
     6181 aatcgtcact aagccgcttg tccctccgta ttcatctttt aagatagcaa tatgctttcc
     6241 cttttttcgc atgatcgtca ataatttttt tatagggatc gtttcagttg ttacgatcgg
     6301 ctcttcaatc aactgttcta ctgtcagctg gtcatcttta taagattgct tgatcaaatc
     6361 tggcaaggtg acataaccta aaacattgtc ttttgattct aagataaccg gatagcgagt
     6421 atgtccttgt tcaatcgaag cttttatcgc ttctttgacg gtttctgaag cttcgaatac
     6481 ttgcatggac gtacggtcta cttgtatatc ttttgccaag gtatcatcaa attcaaacac
     6541 gttttccaaa taatgatatt cttctttggt caactctcct ttttcataag aattcaatgc
     6601 gactaatgtc agctcttctt gagacaatgt ctcttctcct tcagatacga gacgcacacc
     6661 taatagcttc cctagccctg cagctgaatg attcaatacc caaataaaag gaaacattac
     6721 tttataaaaa taatgaagtg gtttaactac gaacaagacg accttctccg tattgacgat
     6781 actatagctc ttaggaagca gttcgccaat aaccacatcg atatacgtaa ctaaaataaa
     6841 tcccagcgcc acagagattg tactgctaat agccgaacta aaaggtagtt taccgatcac
     6901 tggttctagt aaagccgcga cagatccttc accgacccac ccgatgatca aaccggttag
     6961 cgtaattcct aattgactag ctgataaata gttatctaaa tggtgtgtaa catgaagagc
     7021 tagcttggcg ttctttacat tttccttttc tagttgttcc aatcttgatc gtctgatttt
     7081 cactaaagca aattcacttg ctacaaaaaa tgaagtaatt gcaatcatca aaaataaaat
     7141 aatgaaattg ataaccatat tctcccgcct ttctcttttt ctattgtacc ctttttctac
     7201 aaaaagattg aaaataatgc gtattcatat ttaggaataa aaaaggagat gcggcatttt
     7261 tcagccacat ctccttatca cttcaaaatc attgcgttac ttgttgctaa acagcttttt
     7321 catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc aggtcaaagc
     7381 gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg ataagcggaa
     7441 cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc gaggtagcga
     7501 tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct agagttgtca
     7561 cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct cgccctggtg
     7621 tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa tcatggatga
     7681 cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac agattcactt
     7741 ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat ggctcatccg
     7801 tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta tctaaaatcg
     7861 aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag aagaccacat
     7921 ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc gggatataat
     7981 ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt tcccaaataa
     8041 acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct cggaagaatt
     8101 tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag gtaactaaat
     8161 atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt gctgttgtca
     8221 tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga gacgttcgga
     8281 atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct gggatataca
     8341 tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt ttcaacttga
     8401 ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca atgactgcag
     8461 cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt tggaagaatg
     8521 gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac aaccacgcag
     8581 cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc agacgaatat
     8641 ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc gtagccagga
     8701 aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc agctggatag
     8761 cataataagg attcagtgca cggataactg tccagtcttg actgaagttc attaatccca
     8821 ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt ccgacaatat
     8881 ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg ataatgacga
     8941 tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc aagccttcaa
     9001 tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag gcagctcctc
     9061 cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg tagagtgaga
     9121 aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat ttgattgttg
     9181 tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa tcaggattga
     9241 ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc ggacttgtcc
     9301 cgatgtctcc gtagacgact cccatcgcta caagcaggcc agccattgat atctttttgg
     9361 tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat cctttaggta
     9421 agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat tgcttctaat
     9481 tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt tctttggacg
     9541 ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg cacccaataa
     9601 tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta aaaattcgcc
     9661 gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg ggacttcctt
     9721 tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg accataaaaa
     9781 attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta cgctttattc
     9841 agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg taacaaaagt
     9901 gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg ttgtgaaatc
     9961 agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat attttgccgt
    10021 tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga ggttgtaaaa
    10081 aagctgttct gcatcaagta ataagaacag ccaagtaaaa acagctccta atgtttttaa
    10141 cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc gaaaaaaaag
    10201 gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt ccccctgtca
    10261 ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc atttttcgtt
    10321 gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg tttatcgatt
    10381 tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac tttattgaca
    10441 cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat caagttgacg
    10501 aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc aactttgact
    10561 tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc atcagctgaa
    10621 gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc atcgatgatt
    10681 ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg ttcgatctgg
    10741 atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc aatcgttgaa
    10801 gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc ttcagttagg
    10861 atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat accatctttt
    10921 gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg gatatcagtt
    10981 aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc gactggcgct
    11041 ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact tgcttgggaa
    11101 gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg aaattcttct
    11161 tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc gatttcccga
    11221 cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa attgtagtgg
    11281 tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg ctctcctaga
    11341 ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa acctgatcca
    11401 tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc caatttacgc
    11461 ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt ttcaagatct
    11521 tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc tgcaaatttc
    11581 tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc gcgagcaagt
    11641 ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa gatttctttt
    11701 ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc tactgcttga
    11761 acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa cagcaatgca
    11821 ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt gatggcttgt
    11881 ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac tgttggattc
    11941 aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc gtcaaatggg
    12001 atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc tggcgagcag
    12061 tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa gccttctgca
    12121 aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc cgtacttgga
    12181 cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat tttttcttca
    12241 taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc cacagctgca
    12301 cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg ttttgctagc
    12361 tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac ttgtttttct
    12421 gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa atgaaaactg
    12481 ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct agcttgatca
    12541 cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga atcccactct
    12601 ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt aacgttgaac
    12661 gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt tcattaggcc
    12721 gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt ggttgatttc
    12781 ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat ggcgagcgta
    12841 ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca cctctttcga
    12901 ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg caggtcatgt
    12961 gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt agttaatcat
    13021 taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt ggtaaactat
    13081 aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt ccatgattac
    13141 aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa aagaagtttc
    13201 tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt tcttaaaaaa
    13261 cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg gcttagctgc
    13321 tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc ctgacccaga
    13381 ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga gccattctgt
    13441 tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag aagtaccagg
    13501 atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg gtgaaaaaca
    13561 caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg accatatcaa
    13621 cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa aagaaggcgt
    13681 attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa gaaagcttta
    13741 taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc actaatcgaa
    13801 atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg atacgcatct
    13861 tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga tagaatagtt
    13921 tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct gcttcagcta
    13981 gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta tgcagatgga
    14041 tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt ctcataaagg
    14101 aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg taaaggggat
    14161 gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc agttcttctt
    14221 ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta ttggtttctt
    14281 ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca gctcgtagta
    14341 aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca aacttgctag
    14401 cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc cgtgtctctc
    14461 cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt gtttcatcaa
    14521 atcaacatac gtgtctgtta ttgtttcctt atatccctta tttttcagca gactaacaac
    14581 tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt cttctttttt
    14641 cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa ccaactctcg
    14701 atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta gttcttcctc
    14761 tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg gaaaagattt
    14821 ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac ccggcgtgac
    14881 gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg cgtactccag
    14941 accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct ggacattcgg
    15001 agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg tacaattgaa
    15061 atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat tgataactgg
    15121 ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt gggatcgcag
    15181 ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca tacggtcggc
    15241 aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt ctttttcggt
    15301 tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca accgctccgt
    15361 cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg attactaaga
    15421 ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact tgaaaactcg
    15481 ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc tccatgactc
    15541 catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct ccgcatgctt
    15601 gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat tttcgacaat
    15661 gacattgata cagactccca tgaaaattga cgagttgcgg gcttttagcc tttgcgtgca
    15721 aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga tgtgctattt
    15781 ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca aattgataga
    15841 atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc tgctccatcc
    15901 cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg gatacccctg
    15961 caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct tcgtctactg
    16021 ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg aacaaattta
    16081 ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga gcaatttatc
    16141 acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata aaagtgaggt
    16201 gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt atcgcctggt
    16261 tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat ggaaatcttt
    16321 gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca ttagccgtat
    16381 atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta atattagcca
    16441 tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag aaagcggcag
    16501 cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc tttttttatt
    16561 gtattacata catttttgtg tataattata ctatcgtttg caatggatag atgattcaca
    16621 ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt ttttcaaaaa
    16681 ccagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg tataaataac
    16741 tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa ccctgtggag
    16801 tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac ttttttgctt
    16861 ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt gatgctaagt
    16921 tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct ttttatatct
    16981 ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat ctagaccgct
    17041 cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt tcagttgccg
    17101 tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat gcagatgaaa
    17161 aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc ttcaactggt
    17221 ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt tacgccggcg
    17281 gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa ttagtcatcg
    17341 tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc gacgcgatca
    17401 tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc gatccttatt
    17461 acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa gcaaaaagtt
    17521 tagcagatct ttctggagca aagatcactg ctcagttaaa tacattccac tatactgtga
    17581 tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca gcaatgagaa
    17641 cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa ggtgtaacgg
    17701 caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat ggcttccaga
    17761 caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg gatatccaaa
    17821 aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt atggaccaag
    17881 cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc atttctgatt
    17941 tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga cttaccttgt
    18001 tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt ggtgtcttcc
    18061 gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc ggaaatgcac
    18121 ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa gcaatggtca
    18181 tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt gccgctttgt
    18241 ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg gggatttttg
    18301 cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat ggccaaacga
    18361 tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca ggaaatgagt
    18421 ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct gatttgttct
    18481 tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt acgatcgtcg
    18541 ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg gtagaaaaga
    18601 aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca aatttgcagg
    18661 aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta agaaaaagct
    18721 tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt gaagttgtga
    18781 cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc aatttactgg
    18841 aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct cctaaatata
    18901 atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac ctgttcaaca
    18961 atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa cgagataaag
    19021 aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat cgttatatcg
    19081 aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt gctcgggcat
    19141 tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta gaccctgaaa
    19201 tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg acaatgctta
    19261 tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt tttatggata
    19321 aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg aaagaagaac
    19381 gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg attgggacaa
    19441 agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg taaatttctg
    19501 aa
//
LOCUS       DANKMN010000058        18655 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000058 DANKMN010000000
VERSION     DANKMN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            150..278
                     /locus_tag="PW224_002023"
                     /pseudo
     CDS             150..278
                     /locus_tag="PW224_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            392..631
                     /locus_tag="PW224_002024"
     CDS             392..631
                     /locus_tag="PW224_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908695.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            743..937
                     /locus_tag="PW224_002025"
     CDS             743..937
                     /locus_tag="PW224_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908696.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1023..1694
                     /locus_tag="PW224_002026"
     CDS             1023..1694
                     /locus_tag="PW224_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2908697.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1747..3723
                     /locus_tag="PW224_002027"
     CDS             1747..3723
                     /locus_tag="PW224_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2908698.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3738..4490
                     /locus_tag="PW224_002028"
     CDS             3738..4490
                     /locus_tag="PW224_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2908699.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4506..5261
                     /locus_tag="PW224_002029"
     CDS             4506..5261
                     /locus_tag="PW224_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908700.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5274..5534
                     /locus_tag="PW224_002030"
     CDS             5274..5534
                     /locus_tag="PW224_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908701.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5531..7621
                     /locus_tag="PW224_002031"
     CDS             5531..7621
                     /locus_tag="PW224_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2908702.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7648..8112
                     /locus_tag="PW224_002032"
     CDS             7648..8112
                     /locus_tag="PW224_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908703.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8109..8351
                     /locus_tag="PW224_002033"
     CDS             8109..8351
                     /locus_tag="PW224_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDL2908704.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8369..8671
                     /locus_tag="PW224_002034"
     CDS             8369..8671
                     /locus_tag="PW224_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908705.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8741..10804
                     /locus_tag="PW224_002035"
     CDS             8741..10804
                     /locus_tag="PW224_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908706.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10804..13416
                     /locus_tag="PW224_002036"
     CDS             10804..13416
                     /locus_tag="PW224_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2908707.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13413..15944
                     /locus_tag="PW224_002037"
     CDS             13413..15944
                     /locus_tag="PW224_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908708.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15994..16326
                     /locus_tag="PW224_002038"
     CDS             15994..16326
                     /locus_tag="PW224_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908709.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16327..16920
                     /locus_tag="PW224_002039"
     CDS             16327..16920
                     /locus_tag="PW224_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908710.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17083..>18655
                     /locus_tag="PW224_002040"
     CDS             17083..>18655
                     /locus_tag="PW224_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL2908711.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQR"
BASE COUNT         6891 a         2925 c         3836 g         5003 t
ORIGIN      
        1 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
       61 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttcaagcg
      121 taagaaaccg ttaggtttag ctaagtggtg agtagttcac aatgacgctt acgaatgata
      181 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagcg
      241 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
      301 agataaaggc ttagaagaag tcacgacgaa taataaaaag gtagtattac cttaggcatt
      361 gagtaaggaa ttcatttagg aggaaaatta gatggcgcga ccagttttag aggcgatgga
      421 tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt attcacctaa
      481 agatgtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat tacgaaaaga
      541 attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac ggccagtaat
      601 taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat cataggcttt
      661 tttatttttg caaaaaacaa ggaaggtagg aaaactcatg aattatgaag aaatcgaaaa
      721 cagaaaaaaa gtgtcgaaat aaatggaaga aaagttgcta aaaacgatga aacaaaaaca
      781 ccttaaaaga ttatctgtca tgcaatatat caatgatatg cagataacag gaaaagagaa
      841 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg agacgaactt atgtgaaaac
      901 aagttctaat tgtcaactat tgttagaagt ctcatgacat tcattaatag aattcatttt
      961 taaaactttt tctatggaaa tgagaaaaag attttttgtt ttgtctgaaa gggggtagct
     1021 ggatgagaaa atggttattt ggttttttag gagtcgcttt aatcgtcttt tgcagtgtgt
     1081 ttggctatgt tagctaccaa aaacatgaag gagaggtgtt taagcaaaat atagaaaaaa
     1141 agatgcccgt agatcaaatc aatgcacatg caaagagtta taaggaggat gcaacgaacg
     1201 tcaataatga tatgtctttg gggcaaatgt tgtccataca acaagaagca atcgaaatgg
     1261 gcgtcaataa acaagtattt gcacaaattc agattcctgc acttggttta gcattgccaa
     1321 tttttaaagg ggcaaatcaa tacactttga gtcttggagc ggctacttat ttttatgaag
     1381 atgctgaaat gggcaaagga aactatgtcc tagcaggtca taacatggaa atgccaggtg
     1441 ttttattttc tgatattcaa aagttgtcat taggggaggt gatggactta gttagtaacg
     1501 atggcgtcta tcgttacaag gtaacgcgta aatttatcgt acctgagtac tttaaattaa
     1561 tcgatggcgt tcctgaagag aactctttct tgtctttgcc aaagaaagga gagaaaccct
     1621 tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac gtggtacagg
     1681 gagaactaca ataacaaggt tttaatcaac gtgagtcgtt gaaatatgaa atgaggagag
     1741 taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttattgg aatgttagga
     1801 atcattgcag gctgctttca gggatatcga gcatatgcag aagatgtcac gcaaaaaacg
     1861 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg aacgcaattg
     1921 aatgttgata ttgacgggat caaaaacgat ggttatacgc acgatcaata tcctacaggt
     1981 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
     2041 gatcaagtat taccgagaga agattctgat ctaaccaacg cgaaagtgga cgagattgta
     2101 caagatgtag aagacaaggg atctaacagt gagtacgtca agaatgcaac caacaaaatt
     2161 acttcagctg tagatgaaaa tggtgagatc acttttactg atcaaccagc ctatgtcaat
     2221 tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt agtcacacaa
     2281 aaagcaaaac caatggttgt gattgcgcca atgacagata aaacaggatc aggattttta
     2341 aaaaatattc atttgtatcc taagaacatt gtttctaagt tgagttttga attaacgaaa
     2401 tttggtgacg atggcgcaga ccaaagcaaa caaacccttt taaaaggggc aaaatttgag
     2461 ttatacaagg gcgaacctgg aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa
     2521 ggaaagctga ctgcgactga tttgacgtta ggaaaatact attttgtgga agttccttcg
     2581 gaagttgtag tgggatcaga tcaagaacca acagcagatc aatatttatt aggcgcagac
     2641 gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg cgtgacaagt
     2701 gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac agtaacaaat
     2761 ggaacaggac aagaacattc tttccaaatt ggtgacgcag taaattatca aggaacgatt
     2821 catattccaa cagacattgc aggtggcgca gacggtatca cagtaaacgg tgtgaaaagt
     2881 gaaacttctc cttacagtgt atttaaatgg ggagatacag caggacaggg actttcttat
     2941 gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatctgtggt attaaaagaa
     3001 aatacagatt ataaaatcca aaattcagaa aatggctttg tgatcgattt tatcgtgaat
     3061 aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca aatgacttat
     3121 aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag cgtggatttc
     3181 gtttataaca acaatccatt taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt
     3241 gtgacttatg gtgcaaaatt cttaaaagtc gatagcggac tatttggaac aggaatcaaa
     3301 gcaacaccgc ttgaaggcgc agaatttgct gctaaaaacg cagaaggaaa atattatggt
     3361 ggattagttg atacggataa agacggtgtc aaagaagcag tatgggtaga cgatgtagca
     3421 aatgcagcga tcctaaaatc agataaagaa ggtcactttg aaatcactgg tttaactgaa
     3481 ggtgaatatt cactggaaga aacaaaagcg cctgaaaact atcaaaaatt aacgaaagag
     3541 atctctttca aagtagacaa agattcatac aaagaagaaa accgaatcac gatcaaaaac
     3601 aatcaaaaag cttccgtacc aatgaccggt agcaacggtt ttcaaacgta tgtattaatc
     3661 tcttgcttgt tgctaggagc aggggcactt tctgcagttg tttactttaa gaagaaagcc
     3721 tagaaggatc aaaaccaatg attatcagga agcctaagaa aaagcgaatc atgggtaaat
     3781 ggatcattgc tttctggtta ctttcagctg taggtgtctt acttttgatg cctgctgaag
     3841 cgagcgtggc gaaataccaa caaaatcagc agattgccgc aatcgatcga acaggaacgg
     3901 cagcagaaac ggattcttct ttggatgttg ctaagattga gttaggcgat cctgttggta
     3961 ttcttacaat tctaagtatt tctttaaaac taccaattta tgacggaacg tccgacaaaa
     4021 ttttagaaaa tggtgtaggt attacggaag gaactggtga tattacagga ggaaacggta
     4081 aaaatccttt aatcgcaggt catagtggac tttataaaga taatcttttt gatgatttac
     4141 cttcagtaaa aaagggagaa aagttttata tcaaagttga tggtgagcag cacgcttatc
     4201 aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca aagaaacttt gtcacttact
     4261 tagaatcaaa tcccaatgaa gatcgagtaa cgctaatgac atgtacgcct aaggggatca
     4321 acacacaccg ttttctagtg tatgggaagc gagtaacctt tacaaaatca gaattaaaag
     4381 atgaagaaaa taagaaacaa aaattatctt ggaaatggct tttaggaagt actgtttttc
     4441 tttcagtcat gatcattggt tcgctatttg tttataaaaa gaaaaaataa aggagggtaa
     4501 ccaacatgag aaatagaaaa tggctatttg tttgtgcagc cgctcttttt tctttcactg
     4561 cctacacaca gttgacgttt gcagacagac aagaaagcac ggatagcaca gaacaaacaa
     4621 tccaaacgtc agatagcagc acgattcaaa gcagcgatga aaccgtcgat tcgtcaacag
     4681 ttgattcttc agaagaacct aaaattgctg aagacgatca aatagtcaaa cagaccattc
     4741 acgttcaaaa gattatttct tcagaacaat tagacaacca ggggcatgaa aaacaagaag
     4801 gagtaaatgg cgcgaagttt tctgtttatg atgtatcgga cattttacaa aagatggatg
     4861 ttaaggatct cactactgac caaattgaat cccaattaaa aaatcgagtg aaaaaactat
     4921 ccagtgatca attgaagcta gtttctaatg aagaaacaaa gacaattgat caacaaacag
     4981 gtgtctttga attctcggta gaagtacagg caaatcagaa acaagcttat tatattgtca
     5041 atgaatcaag tcctgaaaac atttctaata gcgaggatat tttgctatta acacctgttt
     5101 ctgataaaaa cggtgaattc ttaaaggatg tgtggatcta tcctaaatca gaagcttcgc
     5161 aacctaaaga agaagtaaaa aaaatcgttt ctactggcgt aaagaaaaac ttctttgaga
     5221 attgttggga ttttgttact cacttgtttt ctagagatta aggaagaaat aaaatgaaac
     5281 tgatcaaatt attattattc aatttttctt gtttaaccgc agtctttttg tactgcggtt
     5341 tattattttc taaactatct attttgccaa gctaccaact atacctaatg aggattttaa
     5401 acataccagc tgcttccatg ccgctagata ttatggaaaa gctcattagg aatccattat
     5461 ttactacgat caatttgatc gttattgtcg gaatttcact agtcatttat aaaaaagagg
     5521 ggatgaaggg atgaatttta aacactcgtg gacaagacgg ttaaaatatg tttttcccat
     5581 aatgatgatg ttaggtacat tgtttgggtt aaaaacagcc aatgtctttg ctgaaaaagt
     5641 tattgtcgat ccgtcaaatc caatccagaa cgtaaagaat aattttatta ttccaaaaga
     5701 tgttcctaat gcaaaaacaa atattactgt aaacggagcg tcaaaagttt acaaatatca
     5761 tgttgatgcc aacaaaggtg gttttacaga cgactacgta ggattagtgg agggaaacaa
     5821 aaatatacgc tatccttctt tccaaaaaga atacggtgaa acaaaccttg ttttagaagg
     5881 cgtaacaacc aacaccaaag tcaatattga ctatggaaaa atcggtacgt ataacgggaa
     5941 aaaagtcaat atcaagctag ttctatctaa tattcactta tactcggata cgttgccgtg
     6001 gaatctacta gataacaact atacaaaaac tcattttcga gacgatggct ataaaaatac
     6061 gaatggtgca atgagtaaat ctaaaaaaag aacagtactt tggatctcag acaatctttt
     6121 ctcaggaatt gtttaccatt caacacaaat gaatgtgcag ctagtagcca catatgagga
     6181 cggtagcccc gttcaatttt caggagatac atttatcagt tttaactctt tgaaccctgc
     6241 aggaggaaaa agcacggatc ttaaaggaga atatgcccac tatgacaaaa tgaatacgac
     6301 agattggtat gtcagaaaag atacggttct ttcagaattt aaatctttct acaacaatct
     6361 gaacgtggta ggtggccacc caggaggaag ctctaagctg acacaagcgg ataatgattt
     6421 caataatttg catgataaat taggagatcc gaagtttggt caaggtactg tgtcctttaa
     6481 aatatctgaa gcaaatccga cgtttgtgat tggttcttct aatgttcaaa cctggtttac
     6541 tttatcttct gcgacaatct ttagtgttgt tcctgatcaa cctgaaaaaa caggggttga
     6601 taaaaatgga aataacgtga acgataaaat gttgcaagta ggagacacga ttcaatatcg
     6661 aatcaaacag aaggtgaatc gattaggagt ggatttacta gcggtttacg atcgatttga
     6721 gttaatcgat aatttaccaa aagaagtaaa ttacgtcgat gcacacgttg aaagtggtac
     6781 aaataaaaaa ttcgatgtat ctggtgaagt gacctatgac aaaacaaaac accaggtaaa
     6841 atatgcagcg aaagctgata cattaaaaaa tcgaatgaag tataacggcg aaacttacga
     6901 attagtgatc aatgtaaaag tcaatgaatt ggccaatcaa aatagtgtag ccaaaaatca
     6961 gggaacgtcg atcattaata aagtagaaaa ggatacaaat atcattaccg tttatttccc
     7021 taaaattcca gtgaaagaag tacaacaaaa tggaaaagat gtaaacggac gaaatgacgg
     7081 taagaagggt acgccgacag ctcctttaaa cgcaggatct gaagtacaat atctagtcac
     7141 acaaaaatgg cacacaaaag gcgtagacgc tgcgtcagat cactacaaac aatttagtat
     7201 tcaagatcca attgaagctc gtttgacata taaagaaggt tctgcgcaag taatagataa
     7261 gagtaaagga aaagatatta cttctgaagg cacactaacc tatgatagca attcaagaac
     7321 gctgaagtgg gaagcttccg ctgacttctt aagtaacaat cttttagatg gacgagaaat
     7381 tcagctgata tttacagcta aaactccatt acaatcagaa aaaaatattg ataaccaagc
     7441 cgtagtagca gtagataatg tttctaataa aacaaatgtt gtgacaattg gggttgatcc
     7501 taacttgcca caggtcattg ttcctaaaac aggttctaca catttagtaa cgatttcagc
     7561 ggtcagctta ctattacttg tgttagcaac ttttagttat gcggttctga gatacattta
     7621 aaataaaaaa acgaaagaat gaataaaatg aaaaagttaa taatagtagt tacttccttg
     7681 attgccatga ttgttatttt agtcggatgt agtaatgaaa aaaataaaca aactaatcaa
     7741 gataatggcg tattgaaatc aggaactatg tggaaagaag aagtaggtgg tcttgtttat
     7801 aatttgaaaa ttatagacga gactacctgg gaatactctg agagtgtatg gcatccagat
     7861 cctgtacaaa taacagtcaa gaggcaaaaa gactataaag gtttagaaag atacaaaata
     7921 gtagattcag ctggtgttag ggaatttata aacaaatctg attctttatt tattgttgtt
     7981 ccctatgaga agaatggcgt aaaaaaaata atttttcttg gatctagcaa ggatgaaaag
     8041 cgaacgaaag agaaattgat tcacgatgga tcacaatcca ataaatataa gttacaaaag
     8101 actagcgagt gattgctagt ctttttattt gtcctgaagg aggaatcaca gtggcaaaag
     8161 ctaaaacgcc atttgaaaaa gccgcgtcta aaatcttaga acgccgcggt gtatctgaaa
     8221 cacagtggaa acaagaagtc cttgaaaaag cacaatttga cctattacgg ggaaaagaca
     8281 aggacttaga aggatatatc tatcaacgtg aacgtgaaaa gcttgttttc gatgaaatta
     8341 ttaaacacta aaaggagcga acaattttat gtttgaatca attacaatga attttttaac
     8401 acaaccgaaa gtagtagctg cggttgatat ttttagagca ttacaaactg ccgcaactag
     8461 tttaaaaaac aatctgatga tttttgctgc tgctgcggcc gttgcttcca ttgttattgg
     8521 gggtcttttc ttcctatttg gacgaagagg agctgaaaat gggaagatgc acgtaggcta
     8581 tgtattgatg ggggtaggag ttattttagc agcagcttct attgtggttt cgttatttgg
     8641 attgtttgga caaacacctc aaggattata agaaagtaaa aaagtaggag taagaagtag
     8701 tcgatacttt ttactccttt tttatgaaag gagaatactc atgtacttta cgattgcagg
     8761 caataccaaa acagataaag agttaataaa aaaagcaacg aaggaagtag gaaagcgtac
     8821 ttctttttta ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac agctacatgt
     8881 gttagggaaa gtactggaac acgaaagtcg atcttctgtt aagttgactc tcacgttaca
     8941 tgacacagaa ggaaaaatag agaaagctgg tacagaatac gtttttggcc cactggatta
     9001 tcaaatttca gaaggtttct atcctacatt gatggattta atccgccttg atgtgtttaa
     9061 agaaacggat gaaactatga gtgtggaaga gtcttatcga aacgaaaaac ttttacaagt
     9121 tttagaagaa gagttagaga gcgaccaaag aaaagtgaat caagggagtg aagtcgaagt
     9181 aaatgaagaa gtaccatggg aacgaccaat atttccaaag aaggagtcaa aagaaaaaga
     9241 gacagtctat gttgacgaac aagctgatat tccgactcag aaatcgatat ttgaggaaga
     9301 gaacgatgtg atgggtcaat tggaggtaga tccctttgaa gaaacaatgg aggatgaaga
     9361 gacggccgaa gtagaagttg atccagttga agcgaatgaa gagtcaacat ggactgaagg
     9421 ggattctcag gaatatattg aaccacttat cagtgaagaa gtagaaaaaa acgaaataga
     9481 gatacaaaag gcaactgtat ttgattatat tttggatcaa tatccgccag agcatgaatg
     9541 gatgaaagaa aaaaacaaat tatttttaaa tgagctttat tcgaaatatc aattacccca
     9601 aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta aaggcacact
     9661 tttagaacag ttagagtctg tccaatcaac aaattggcat gagcttgcac aagaagattt
     9721 attagccacc tttgaagaac gtcaacaaga aacagatcaa gaaattaatg agtatgcgac
     9781 ggaacagtcg tctaattggg agaaagcaaa agaggaacta aataaagaag aacagcgagt
     9841 gattgaagaa gaagtacaaa aaattaaaga gcgatacgaa aagaaacgcg acaatgcgta
     9901 ttccttgtgt gtagcaaaga tcaataattt tactcagacg aatcaagaag cgttatcgaa
     9961 agaaaaagaa aacttgttaa aagaacgtat taaagaacga aaagaagaat tttaccgagg
    10021 tttgcgtaca gataagctaa agattagcaa agaattaaat gaacaactga ataagctata
    10081 tcaaacaacg actgaagttc ttatggaaaa atacgaaaag atccagcaag aattacaagt
    10141 gcaaattcca aaatggaaaa aagagtatga aattcaacaa caagaattga aagaacaaga
    10201 agaaaaacaa cggcaaaaag aaaaagagcg cgaacaatta gaactaaaaa aacgtgaaca
    10261 ggatcttgcg gaacaacaaa tggcaataga aaaacaacgg ttaaaccaag aaaaggaaaa
    10321 agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac aagctcagtt
    10381 aaattatcta gcacaacctc aaccaacgcc ttcagtagct gctgttcaaa ccccaatgaa
    10441 caaccaaaca aatctaactc cttcaccaca gcgttccttt aacggatgga tggtgggatg
    10501 tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta atcatatcaa
    10561 tcaatcttca caggctgatt ctgtggccac aacgacgtca atcaaggaag aagcccaaga
    10621 acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta ccaatcagtc
    10681 aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg ctaaagaagg
    10741 atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
    10801 ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt agaaaagaat
    10861 aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc agaaaaacat
    10921 gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc tttagagtat
    10981 ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg aagcttacaa
    11041 ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga ttcagctggt
    11101 ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat ttcgacagtt
    11161 tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tcctctagca
    11221 gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca gatttatccg
    11281 caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat cacacattac
    11341 aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg tacctctcga
    11401 tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg tgcagaaaaa
    11461 caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag taaagatgaa
    11521 ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact gcaatcaatg
    11581 agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat agatactgca
    11641 acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc ttcagataac
    11701 aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc gttgttagaa
    11761 caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt tgagcttctt
    11821 tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa acagttgaat
    11881 gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa acaatatggg
    11941 gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc aatcacggga
    12001 ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac gattgattat
    12061 aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct tttaaatcaa
    12121 cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg tgtcaaagta
    12181 ggctataaag gattttgtga aatcaatttt gattgtcatt tagttgcagg tgatcaagtt
    12241 tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt ttctcaaaga
    12301 gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga aacccatttg
    12361 ccagaattag aaaaagaagc cgttctaaca ttagatgagg agaaaagtaa cttattagta
    12421 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct tacacctgat
    12481 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata taaagaatta
    12541 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca ttttatttta
    12601 gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac ggtcacagct
    12661 ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta tgcgaagtat
    12721 ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    12781 acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa
    12841 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    12901 gatcgtatct taactcctga aagaatttct acttttttag aaggtgaaac gattgtactc
    12961 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    13021 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    13081 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    13141 attgattatg attatttcct accagatgaa tatgctagaa atctctatca tggactgcat
    13201 gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atctcttgag
    13261 aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc agatcgactt
    13321 caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat tgggaaacgg
    13381 ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt ttttctggca
    13441 tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa accgtaacga
    13501 aagtcttaca agattatggt aattatctgc agtatcaaga tgtatttagt tggatttgga
    13561 acacttttct gtggggattg attaaaggat tatataatct caatacttta ctagaaaaaa
    13621 ccatttatca aagctttgat ttaaaagact tattgaacgc cgctggggta aatgcactat
    13681 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    13741 gcttgaaatt ttttatgagt aaacacccac caaaagtcaa aaatatcttg gttaatttat
    13801 ttatggccat gatcttgatt tttggtggga gttctttaat cgatgaaggg ttaaatatca
    13861 gtaaaagctt ttacggagac gtcactgcag caaataaagc agataaaacg ggttcacctg
    13921 cttttcaaat tattcaaaat aatgtgatcg atgtcagtac attactagca actgacccga
    13981 ataaagtaga atccctgcca acagacaaaa gaaatgcttt aacagcgaaa aattttaaaa
    14041 cagcgaatat caatgggatt attactccta aacaagctaa agaaatggcg gataaagata
    14101 agagtggttt atcagattta caaaaggatc ggttaagagc gttacaatac cgattggaac
    14161 tagatgataa tgggaaagaa attcctgttc aaattagtga cgaaggctta gcaaaatatg
    14221 tctatacttc agggtatcgt cgatacagta gtaaaccagg tattatctta gctggtgaat
    14281 cttctttggc ggtggcctat ttatttatcc tatttacgat cgttacttgc attattgagc
    14341 tggtatttaa aaaattctat ttagtgattg ccgcgtcaac ggactttgag acaggccaac
    14401 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    14461 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggcaggaa
    14521 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
    14581 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagtaatt
    14641 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    14701 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aaaaatggat
    14761 ttggccatcg aaaagatgtt acccctgata acggggaaga acaatcagac gataaagcta
    14821 caactgtacc taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    14881 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagctg
    14941 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
    15001 aagaattggc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    15061 cttcagaagt caaagaagga ctaaaagatg cgtatggtga aggacaagtt aatgcagcag
    15121 taaaagctaa tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt ccttcagtca
    15181 aagatcacca agaagcgttg cactctaatg aaaattcgat tgaagagaaa aattccatag
    15241 aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat ccagaacaat
    15301 atcgctataa acctaacagt gaccttgaaa atgagaagcg aatttcagat ttatcaaaag
    15361 taaatcaaga agacaaagca gaaaatgagg gacagttacc aaaaactgat ccagaacaat
    15421 atcggtatag atctgttaac tccgaaaatg aaaaacgcat ttcagattta ccaaaacgaa
    15481 atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat ggaatcgaag
    15541 ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta ccaatgaaag
    15601 atagtcgttt gaatcaattc aaggagaatg atcaaagctt tgttcaccaa tcaactgcag
    15661 gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    15721 acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt gtgacaagag
    15781 aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca aatgtggcat
    15841 ttactgcacc aacaaaaata aatgtgacaa gagaaactaa aaacaggaac aatgcattga
    15901 aagaacaact attgagggat atcaaacagg atatcaaacc ataaaaggcc tacaagttag
    15961 gccttttatt tatatgaaaa ggagaaatca attatggata aatatataaa catgaaaaac
    16021 cctccaagat ttagtggaga atggaagttt ttcgcttttt tctttttaga tctattaacg
    16081 cttggtggct gctttttttg ggcgaaaatg gtcaataatc ttgtccactt accactcttt
    16141 tttgcactct tgaattatgg cttatggatt gcgatagcgt tagtgattgt ttggcgaccc
    16201 tataataacc caggtcaacg acaaattttt gcgattatcg agtctatttt ttttgcggac
    16261 aagaaccatt atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat
    16321 gaataaatgc gagtcaaagg acaaaattca gttgaaagga aatccatttt gcagtttgga
    16381 aaaccagaag agcgctctat cttggactta tttccgtata aatcctgtac agaagaggga
    16441 gtcttaatta ctagagaaga tcattttcaa cgttattatc gtgtagtttc tacagacgtg
    16501 gaaggattaa atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt
    16561 acgtatgtgc caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa
    16621 atcgtgcaaa agcggcaatt attagataaa aatcgtatgg aacagtcctt aggaagaaat
    16681 ctacaacgat taaaaaaata tgaaaagaaa ttagtcgaag aaatccagga attgaaacga
    16741 gcagaacaag aacgtcctga cttaagcttc tttttcgtca ttgaaggaaa gacaatcaag
    16801 gatttacaaa ccagaaaccg tcagttaatg cgatctagtg gcattttagg gttaaaacca
    16861 ctctacaaaa aagaattgat caagattctg tatcgaatga acaatatgaa tgatgagtag
    16921 gaatggagaa gagagaatga aatatccgtt gatatggtta gaaaaagatg ttccgaagga
    16981 agagaccact agaaaatttc ctcacacttt aagcctgggt aagttgggaa aagggcaatc
    17041 ttttaaccaa gtaaagaatc aaacaagaag aggtgactgt acatgttaga agcaattaaa
    17101 acagcttttt caccacaaaa tgatcaaaaa gtacttgatc cattaaagca actaggaaat
    17161 cgggaaatag aaattttaaa atccaaaggg tatgatttag attttttaaa acaagtgatg
    17221 ccacaaggtg ggttaaattt tgatgaagaa tatgtgacgt atggtaatgg ttattcacga
    17281 tgtgtgacag tttatcacta cacaaaaaat cctacgcttt tttggttggc caatctcatg
    17341 aacaatcctc atacagtggc cacactcgat gttagcacag atgaaaagca aaaagtgatt
    17401 gatcaaatca atcgtgcctt agatgaatta aatgatcgat cagataatga acgaaaagcg
    17461 actgaccgaa ataaaagcgc agaagaatac ctggaactag cggaatatgc tcgtaaattg
    17521 acttctaatg gggaaatttc taagcaagtc aaagtgagag tacatgttta cggaaacacg
    17581 attcaagaag tagatgaacg agcgcaggaa ctgatcgaaa atctaaaagg gatggattat
    17641 cgtgcggtga cttatctgca taaaaccaaa cgagattggc aatcgatgtt taccatgttc
    17701 gatgaacaag ctgaatggtt cggaaatatc aaagggcaat cactaccatc gttaaacatt
    17761 ggcggaggtt tcccgttcca ccaccaaacg ctaaaagatc caggagggac acaatatggg
    17821 gaaaccatga caggcggccc atttgtctgg aatgcgacgt ataaaaatag tgtacggttg
    17881 tctactaata ccttgcttct aggaatgatg ggagctggta aatcgaccgt tcttaaaatg
    17941 atcgcggaag cccatttagc tgcaggagac tttatttggg gctttgaaaa gggaaaagac
    18001 tttattcctt tcttaaagga atataacggt attatggtgc gtttagacgg atctgacgga
    18061 atgatcaatc ctttagagat atttgccaca cggacgtatg atgaagcatc cagtttatat
    18121 gacgatggat cagtcaaaga tttgaagatc aatgaagcag cgtcctatca aacacactta
    18181 gacaaagtgg tctatcaggt acagttagtg agtcctcagc taaaaggaac gatgaaagca
    18241 gaatttaaga cctacttaaa tcgtttttat gaagagtatg gaacagtacc acgcggcttt
    18301 acttcctcaa attcgcgttc aaatacggag acgcaagtca caggtaaaga tccagaagct
    18361 tatccgacgt tcaaagaatt tttagacttt ttaggacaac tggaattacc aggtgcttcc
    18421 caagagaaaa agaatcgaaa agaagaaatg gaatccatcg tagaatcctt atgtgagacg
    18481 tatggcatga tttttgatgg gcatagtacg attcgacacc ttgaccaaca acagttggtc
    18541 ttttttgaca ttgactctat tacaggctta gatcgagggg tacaacaatg tcaaatgtac
    18601 atggctatga cgttgatttg gaatcaggca ttgattcaag gaagaagaca acgac
//
LOCUS       DANKMN010000059        18567 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000059 DANKMN010000000
VERSION     DANKMN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            6..305
                     /locus_tag="PW224_002041"
     CDS             6..305
                     /locus_tag="PW224_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2908712.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PW224_002042"
     CDS             378..1739
                     /locus_tag="PW224_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL2908713.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PW224_002043"
     CDS             1761..2021
                     /locus_tag="PW224_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908714.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PW224_002044"
     CDS             2035..2787
                     /locus_tag="PW224_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL2908715.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PW224_002045"
     CDS             complement(2881..3486)
                     /locus_tag="PW224_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2908716.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PW224_002046"
     CDS             complement(3539..4513)
                     /locus_tag="PW224_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL2908717.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PW224_002047"
     CDS             complement(4891..5610)
                     /locus_tag="PW224_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2908718.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PW224_002048"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PW224_002048"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2908719.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PW224_002049"
     CDS             7161..8558
                     /locus_tag="PW224_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2908720.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PW224_002050"
     CDS             8639..9079
                     /locus_tag="PW224_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2908721.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PW224_002051"
     CDS             9227..9553
                     /locus_tag="PW224_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2908722.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="PW224_002052"
     CDS             9554..10303
                     /locus_tag="PW224_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908723.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="PW224_002053"
     CDS             complement(10383..10838)
                     /locus_tag="PW224_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2908724.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="PW224_002054"
     CDS             complement(11265..11540)
                     /locus_tag="PW224_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908725.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="PW224_002055"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="PW224_002055"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL2908726.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PW224_002056"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PW224_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2908727.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="PW224_002057"
     CDS             14770..17436
                     /locus_tag="PW224_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2908728.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="PW224_002058"
     CDS             17800..18552
                     /locus_tag="PW224_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2908729.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
BASE COUNT         5965 a         3212 c         3833 g         5557 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatg
//
LOCUS       DANKMN010000060        18528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000060 DANKMN010000000
VERSION     DANKMN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            512..1069
                     /locus_tag="PW224_002059"
                     /pseudo
     CDS             512..1069
                     /locus_tag="PW224_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1127..3166
                     /locus_tag="PW224_002060"
     CDS             1127..3166
                     /locus_tag="PW224_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL2908730.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3275..4426)
                     /locus_tag="PW224_002061"
     CDS             complement(3275..4426)
                     /locus_tag="PW224_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2908731.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4580..5110)
                     /locus_tag="PW224_002062"
     CDS             complement(4580..5110)
                     /locus_tag="PW224_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2908732.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5209..5559)
                     /locus_tag="PW224_002063"
     CDS             complement(5209..5559)
                     /locus_tag="PW224_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL2908733.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5716..7245)
                     /gene="ahpF"
                     /locus_tag="PW224_002064"
     CDS             complement(5716..7245)
                     /gene="ahpF"
                     /locus_tag="PW224_002064"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL2908734.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7255..7818)
                     /gene="ahpC"
                     /locus_tag="PW224_002065"
     CDS             complement(7255..7818)
                     /gene="ahpC"
                     /locus_tag="PW224_002065"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL2908735.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7962..8624)
                     /locus_tag="PW224_002066"
     CDS             complement(7962..8624)
                     /locus_tag="PW224_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2908736.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8808..10454
                     /locus_tag="PW224_002067"
     CDS             8808..10454
                     /locus_tag="PW224_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2908737.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10445..11527
                     /gene="uxuA"
                     /locus_tag="PW224_002068"
     CDS             10445..11527
                     /gene="uxuA"
                     /locus_tag="PW224_002068"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2908738.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11635..13188)
                     /locus_tag="PW224_002069"
     CDS             complement(11635..13188)
                     /locus_tag="PW224_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2908739.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13336..14624
                     /locus_tag="PW224_002070"
                     /pseudo
     CDS             13336..14624
                     /locus_tag="PW224_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14750..15829)
                     /locus_tag="PW224_002071"
     CDS             complement(14750..15829)
                     /locus_tag="PW224_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2908740.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15932..16372)
                     /locus_tag="PW224_002072"
     CDS             complement(15932..16372)
                     /locus_tag="PW224_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2908741.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16429..16839)
                     /locus_tag="PW224_002073"
     CDS             complement(16429..16839)
                     /locus_tag="PW224_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2908742.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(17019..17453)
                     /locus_tag="PW224_002074"
     CDS             complement(17019..17453)
                     /locus_tag="PW224_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2908743.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17553..18401)
                     /locus_tag="PW224_002075"
     CDS             complement(17553..18401)
                     /locus_tag="PW224_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2908744.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         6382 a         3429 c         3331 g         5386 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      541 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      601 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      661 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      721 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      781 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      841 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      901 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      961 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     1021 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1081 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1141 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1201 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1261 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1321 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1381 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1441 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1501 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1561 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1621 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1681 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1741 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1801 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1861 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1921 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1981 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     2041 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
     2101 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2161 gacaatcgat gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc
     2221 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2281 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2341 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2401 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     2461 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2521 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2581 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2641 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2701 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2761 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2821 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     2881 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2941 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     3001 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
     3061 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     3121 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3181 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3241 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3301 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3361 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3421 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3481 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3541 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3601 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3661 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3721 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3781 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3841 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3901 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3961 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     4021 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4081 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4141 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4201 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
     4261 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4321 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4381 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4441 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4501 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     4561 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     4621 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     4681 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     4741 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     4801 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     4861 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     4921 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     4981 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     5041 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     5101 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     5161 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     5221 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     5281 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     5341 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     5401 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     5461 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     5521 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     5581 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
     5641 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
     5701 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
     5761 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
     5821 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
     5881 catttcgatc gttctatcta gccaatccgt attagggagt aagccgacta gaatgaatac
     5941 tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc
     6001 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
     6061 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
     6121 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
     6181 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
     6241 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
     6301 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
     6361 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
     6421 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
     6481 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
     6541 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
     6601 accgccaatc actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
     6661 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
     6721 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
     6781 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
     6841 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
     6901 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
     6961 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
     7021 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
     7081 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
     7141 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
     7201 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
     7261 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
     7321 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
     7381 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
     7441 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
     7501 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
     7561 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
     7621 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
     7681 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
     7741 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
     7801 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
     7861 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
     7921 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
     7981 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
     8041 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
     8101 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
     8161 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
     8221 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
     8281 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
     8341 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
     8401 gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt
     8461 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
     8521 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
     8581 ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa
     8641 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
     8701 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa
     8761 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
     8821 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
     8881 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
     8941 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
     9001 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
     9061 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
     9121 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
     9181 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
     9241 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
     9301 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgcg ctactttata
     9361 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
     9421 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
     9481 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
     9541 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
     9601 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
     9661 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
     9721 aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
     9781 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
     9841 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
     9901 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
     9961 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct gatatgcctc
    10021 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
    10081 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
    10141 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
    10201 cagacccact attgacagac tttcagcata agctttccga tattcaactg ggtacgcaag
    10261 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
    10321 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
    10381 caggaccgaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
    10441 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
    10501 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
    10561 agaggtatgg cccagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
    10621 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
    10681 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
    10741 agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta
    10801 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
    10861 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttgaattgc cgggatggga
    10921 acctgagcgc ttagctcata tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
    10981 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
    11041 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattacctcg
    11101 aatcgtaaag aatcgagaag atttagattg gttgtgccgc atagtagaca gcccttataa
    11161 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
    11221 tttagcagaa ttttgtcaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
    11281 tttagctaat cgagatttct atgaggcacc gcataaatcc gaatacggtt cattagacat
    11341 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac gaatacatcc gtcctgatca
    11401 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
    11461 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
    11521 taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga
    11581 tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga
    11641 agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat
    11701 atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa
    11761 aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat
    11821 gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc
    11881 tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac
    11941 aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg
    12001 cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat
    12061 ataaaattgt tcaatggcgg tttggacttc tttcaacacg ctatgttctt gataaagcat
    12121 gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt
    12181 tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac
    12241 agtacaaaag gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt
    12301 taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg
    12361 agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat
    12421 atctacagag ggatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac
    12481 gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt
    12541 atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat
    12601 aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt
    12661 ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct
    12721 gccattatta gaaaaggaga tagacaaacc gtacaatgct aaaaatgcat taatcttttt
    12781 cattttcgta tacaaataag aggcactata gtttgtttgc tgacttaaac tgataatcga
    12841 atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta
    12901 tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa
    12961 aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc
    13021 taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata
    13081 ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt
    13141 gctttttttc ttcaagagaa acgttttaat cgcttcttga gtttgcatga aagaggctcc
    13201 tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa
    13261 aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca
    13321 aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc
    13381 ttatcgctta ctataaatca gacagtccac acagaggaga tagaaacagc caaatggaca
    13441 gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat
    13501 tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat
    13561 tcaggagact tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccgct
    13621 ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc
    13681 ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca
    13741 gatggactac cgaacgatca tcatcattta acgccgtata gtcagcggca gacaattgag
    13801 caagattcag ctatcgactc aggaggagat cttgttgaag aaccaacaga taaaccagaa
    13861 aatgaaaata agccagaagt accgcctaca gaaaatccag acggagaaca gaaaccggaa
    13921 atagaacctg gagaagaacc cgatacagaa actcaaccag agccagacaa tgaatcaaaa
    13981 cctgaaataa cccctggaga aaaaccagat gtagacccag aagaaaaacc agatgtgact
    14041 ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat
    14101 aatgaaactg aaaatccaga aaaaccagag gtagccccag aagaaaaacc agatgtaact
    14161 ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat
    14221 aatgaaactg aaaatccaga aaaaccagag gtagatccag aagaaaaacc agatgtgact
    14281 ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa caaaaaaaca
    14341 attcaagagg atggaaaaaa agagagtaaa aaatcaaatc tagcaattct aaaaataaat
    14401 gaagagcagc tgaataaaaa atcacgaata tttgatagcg cccaatcagc tgaaacctta
    14461 aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa aaataaacag ctaccaaaat
    14521 ccggagagtc acaaaacaaa gttatactgt ggagcggaat cattctatta agtatagcaa
    14581 caatgctttc tgcaaaaaga tttaaacaaa acagatctct ctaacatttg aataatgtct
    14641 gtgaacgcat ttttcaaata aaaagaaata tccagaaaat gaccccaaaa taccagattg
    14701 aaatctgata tttggggtcg ttcatacttt atctttcttc atatttattt taagggatat
    14761 ctgtttcttt tggaagatgg ttcgggatct ttttcacttt caatacatct aaaaagaagg
    14821 taaagatcgg aatgccgaca atcaagcccc aaacgccaaa gaaccgttcg ctaacaagta
    14881 agaccacaaa tgtataaaaa atcggcagtt ctgttttgct ggacatcaac ttcggattca
    14941 gcacataaga ctcaattaga tggacaatca aaatcgtaat caggatataa acaacatctc
    15001 ggatgcctcc ttgggaataa gctatgaaag atagaggaat acaagaaatg ataacacctg
    15061 ctactgggat cagacttaaa atgaaaatca tgatcgctaa actcagcagc tgatgaaagc
    15121 caaatacagc caaggcgatt gtagtgagca acgtatttaa aagagcgaca acaaattgag
    15181 cttctaatac caaaccaaat gtattgacaa acttatcggc aaaatagtaa atatcttgaa
    15241 agaaccaaga aaattcactt tttaaaaaca atcttgagaa tacaattgtt tttttctttt
    15301 caatcataaa aaagaaactt aagatgaatg acatagcaaa cgccactgcc atttttccta
    15361 tatcctgtaa atagccaagc gcaatcgaag cgccattttg aagctgttca aaaaaatcgt
    15421 ttcgctcaat ataagtatgg atatattgta aaacaggatc tgcatctttt ggctgatgct
    15481 cgtaaaaatt aataacggac tggatcatgt caaatgtttg atgaataagc atcgggatat
    15541 actttgtgaa tgccagatag ataaggtaaa cgatcagtgt ataaatcatg atagctaaaa
    15601 cacctgtagg gattcggaca aatcgctgaa tgatttgaac aagatgaatc atcaaataag
    15661 taaagataaa tgtgagcaaa atggttgtaa tcatacttct aactaagaaa agaacaaaaa
    15721 cgacaaataa taaaacagtg agacggcgta ttttttcatt cgccaaaaaa cgctgataaa
    15781 cggtcaaatc gacaacctcc aaataacaaa aaataaacac gttattcatt atatgatgga
    15841 atatgaaaat aagaaagagc aaaaagaaaa accgctaaat gacgttatct tatgagttgg
    15901 ttttcctcat aaaatcgttc atctagcagt ttcagccctt tttctggtct tgatgaaaat
    15961 tatctagcat ttttcgggtc agatgttgca gtgtatccaa ttcttcctgt tcaagtggaa
    16021 gataagactt gatcttttgt ggaatacctt ctgcttgttt ttgcaacgca gcaccttgag
    16081 cagtcagcgt aacatttacg attctttcat cacccgtttc acgctgacga ttaatataat
    16141 ttttttctgc taactttttt agtaagggag tcagcgtacc agaatccaga cacaaatcat
    16201 ttcctagctc tttcactgaa gtctttttct tttcccataa aactaacatc acaagatact
    16261 gggtataggt aagattcagc ggcttgagga ggggagtgta gtgtctaatg atttctttag
    16321 aggcggcata taaaggaaaa catacttgat taactaaata tagatcttcc atttttccac
    16381 ctccgctgtt ttactatagt gtatcatagc ggatttacac aaaaaagtct attcactaac
    16441 tgctttcaga gtaacttcta tatttccagc tgtagctttt gaatacggac acactttatg
    16501 cgctgcctct aataattctt gcgtttcttc cagagaaaga ccttcgatag atccttcaat
    16561 ctctacacct aagacaaagc tcgtttcact ttctgaataa agggaaactt gtgcactgac
    16621 agtagaagcc ccagagatat ttttttgctg tttgatcaat tctaatgcac tgttaaaaca
    16681 tgcactgaaa ccagctgcaa acaattgttc agggttggtt gcattttcga cgcgtttacc
    16741 aggagctgca atattcaatt cgaatgtacg atcgggatta tgcacttctc cagagcgtcc
    16801 gcctgtgttg atcattttcg tagaatacat ttttttcata aaattaccac ctttaattat
    16861 attttataca attgaatttt acacaatata attgtggaaa gcaaagaaat ggattggcat
    16921 aagataaata agagaaagca catgaaacag acgcgctttc tcgaatagca aacatataca
    16981 aaaaatatca gagacaaaaa ataatctcat cataaacatt acatatattt ctcaaaaagt
    17041 tgttttcttt cttcaaaaat tacttcacga tggacctgat gaagcatcca taaataacca
    17101 aaaggatctc tgaacattga gttagtcaca ccaaagtcca tcatttctgt catttcttga
    17161 atctctacac agccatttcg taatgcttta ttatgtgtgt cctcgatatc agagacgatg
    17221 atattgaacc agatcgtatt tggatgttca gcagtaggag cagtcaaacc aagagacgtg
    17281 ttttcatcca gcatatggaa tctcgtacca tacaaggtaa aaatgacttc attattgcct
    17341 ctagggtaat ctgttgcctc aatcagttgt atttcatcaa agattgtttg atacagttct
    17401 agtgctttca aactatcatt taatacaaaa tcgatttccg ttcctttatg cataaagatc
    17461 tcctctcctt ttcagtcgaa cagttactag attcttttat tataccagct aaagaaaacg
    17521 cattcaaaaa aatagcggat ttacttacca actcattcga ttggatgata agtaaatccg
    17581 cgtcccatta cttctgctac gctgctgaca atgacaaagg cacttggatc gatttcttct
    17641 atcaatggtt tcattcggga aaattcatga tcagtcacaa cgatcaataa aacctctttt
    17701 gactttcgat tatacccgcc ttttgcctct agcagggtaa ggccacgtcc aatctcagca
    17761 cttaagcgaa ggcggatgtc ttccaaataa tgttcactca tgaccataac agatttcttt
    17821 cgattgatac ctgtttcgat ataagtcatg acaattgaag tgattgcaat agataaaata
    17881 gcataaagaa actcttctat tccaaaaata aacagattaa gagaaacaac gaatgcgtct
    17941 gtaaaaaata agccaatcga agtattcaag tggaaatatt ttttaaaaat gaggggaggg
    18001 atcgtcgtac cgccgctaga agagttgttt ttatataaaa tcgcaactcc taaagcaaaa
    18061 atcgcacttc caaaaatgac agacaacaac cgatctgaag taatcattgt ttctggaact
    18121 attgcaatga ttaaaggaaa aaccaagctt ccgaaaagta ctttaaaaaa gggtttttta
    18181 cctaaaaata gaagagctag taccaacata acgatattca acactaaaat aactatagct
    18241 cggtcaaagc ctaaagcagc ttccagtaat attcctagac cgctgacacc tccagcagca
    18301 atatgatgag gccctaaaaa catattgata gcgactccta atatgaagag ggcaaaaata
    18361 atcttaatgt atgtccatat aaattgtctc gtttctttca tttattcact tccttaattc
    18421 attaataata ctcttatctt attataactt acttttcacc ttaagttaac tttaattaat
    18481 aaaaaaatta ggctagtcta aattattatg tttcattctt ctagaata
//
LOCUS       DANKMN010000061        18342 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000061 DANKMN010000000
VERSION     DANKMN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            489..656
                     /locus_tag="PW224_002076"
     CDS             489..656
                     /locus_tag="PW224_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908745.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            682..1026
                     /locus_tag="PW224_002077"
     CDS             682..1026
                     /locus_tag="PW224_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2908746.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1031..1312
                     /locus_tag="PW224_002078"
     CDS             1031..1312
                     /locus_tag="PW224_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908747.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1415..1729
                     /locus_tag="PW224_002079"
     CDS             1415..1729
                     /locus_tag="PW224_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2908748.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1707..3401
                     /locus_tag="PW224_002080"
     CDS             1707..3401
                     /locus_tag="PW224_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2908749.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3421..4599
                     /locus_tag="PW224_002081"
     CDS             3421..4599
                     /locus_tag="PW224_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2908750.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4562..5248
                     /locus_tag="PW224_002082"
     CDS             4562..5248
                     /locus_tag="PW224_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2908751.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5248..6408
                     /locus_tag="PW224_002083"
     CDS             5248..6408
                     /locus_tag="PW224_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2908752.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNDFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6418..7293
                     /locus_tag="PW224_002084"
     CDS             6418..7293
                     /locus_tag="PW224_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908753.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7290..7601
                     /locus_tag="PW224_002085"
     CDS             7290..7601
                     /locus_tag="PW224_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908754.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7591..7944
                     /locus_tag="PW224_002086"
     CDS             7591..7944
                     /locus_tag="PW224_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2908755.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            7934..8335
                     /locus_tag="PW224_002087"
     CDS             7934..8335
                     /locus_tag="PW224_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908756.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8328..8732
                     /locus_tag="PW224_002088"
     CDS             8328..8732
                     /locus_tag="PW224_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908757.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8744..9352
                     /locus_tag="PW224_002089"
     CDS             8744..9352
                     /locus_tag="PW224_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2908758.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9372..9734
                     /locus_tag="PW224_002090"
     CDS             9372..9734
                     /locus_tag="PW224_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908759.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9737..9919
                     /locus_tag="PW224_002091"
     CDS             9737..9919
                     /locus_tag="PW224_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908760.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            9937..13269
                     /locus_tag="PW224_002092"
     CDS             9937..13269
                     /locus_tag="PW224_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2908761.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            13320..14057
                     /locus_tag="PW224_002093"
     CDS             13320..14057
                     /locus_tag="PW224_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2908762.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14067..16358
                     /locus_tag="PW224_002094"
     CDS             14067..16358
                     /locus_tag="PW224_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2908763.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            16382..>18342
                     /locus_tag="PW224_002095"
     CDS             16382..>18342
                     /locus_tag="PW224_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908764.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAIN
                     KLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGS"
BASE COUNT         6512 a         2723 c         3866 g         5241 t
ORIGIN      
        1 taaattgttc ttattatcta tcatccgttg ctgtctattg tttttttaat tattcacacg
       61 ataactaaag gtgagtgaag agaaatgatt ccattaataa tttcaatttt tgcgctctgt
      121 cttaatgtct atatgattgg atttaaaaat gggcaaaata aaaaatagta gcagccaaga
      181 ataattttat agtgtcactg tggcggaaag ggtagacgct taaaaataag gtcaatacgt
      241 cgagggatag ccttaacgtt ttatgatttg accatgcaat gttcgattca ttgccagtga
      301 ctttggcaac cgagggttgg aaatgggcgc tcaaagtaca cgagcaaggc gaggtcgata
      361 gtaatcgatg gaatcggtgt aggttgcttg atagagctat aactgcatct cgtttatgag
      421 gtgtagtttt tacagattag atcactcttt gagtggtctt tttattttac acaaaggagg
      481 taacaagaat gtatagacca caatacttag aacagaagta tgaagtaatc actgtgcaaa
      541 acggtaacgg tgagatagta cgaaagtata gaagaccaat aaagagcgat acatacaaac
      601 gaaaggaaag caatgaagtt attccattgt atggcaaaag aatagctaag cattaaataa
      661 gattgcgaaa ggagacggaa tatgaccgag gaattctata gatggctatt acagttgata
      721 agagaagatc gtttagttaa gttctatcag tctcctaaat ggcgcaggct tagagagaaa
      781 gcgatgaaac gagatcacta tgaatgccaa gagtgtagaa gactaggtaa gtatcataga
      841 gtagaaaacg ttcatcatat aaaggaagtc aaggatagac ctgacttagc tttagattta
      901 gataatctta tttgtttatg tgttgaacat cataatgaag ttcatggcag atatcttaca
      961 gcattagata aacaagagaa gaagatagaa agctttgcta acttcgatgc aagtgaaagg
     1021 tggtaagtgc atgatcatca atgataatgg cagagagtat gatacagaaa agattgaaga
     1081 gtattcatct tatactcagg gattaattaa acgtttgata tacgttcgct atgtaggtat
     1141 tagggatctg ttatcagata actgttgtag taaatacaaa gtgaatcaag taagagaagc
     1201 gttgaataaa gataataacg tcgaaagaat aaaaaatgtt tttggatata gtattgaaga
     1261 gattaattat tacattgact tcgctgaagc tttcattccg atggtgagat aaccccccct
     1321 taaaataaat cgcaaatttt ttgggggtga tgaaacggag ggggctgtca ggaaaagaga
     1381 ttttttcgaa ctttatcatg aaaggagggc taaaatgttt aaaaacgaat tgtctcaaaa
     1441 tcgctacaga gaaaaattac gccgctcttt aataagccaa ttggaaagtc agaaaacaaa
     1501 tattgagcca ttcttagata atgttgatcg ttatatcagt ttatgggaaa cggcgatatc
     1561 actggaagaa gatatatccg agaacggcat tagactggag aatggtaaaa agaatgaatc
     1621 agtagcgttg cttgtttctg tcaacaaaca aatgggattg atgttggata aacttgccat
     1681 tactcctgaa ttggtaggtg aagcaaatga atcaattcct gagttataag catattgaaa
     1741 attggttcaa agctatagaa gaaggcacta tcaaggtatg caaagagcaa ttattgctaa
     1801 aaaattatct agaagaaaga gtctttacta gagaagatat ttacttcgat aagcagatgg
     1861 tagaggattc aatcaatata ccagcacaat actttccatt cgaattaatt ccgtgggaaa
     1921 aatttctaca atgttttatt tatggtgttc gatggaaaaa agataaaaca ctagtgttca
     1981 atagatatct ttcattaatg ggacgtggta atggtaaaac tggttttgct tcttggaaca
     2041 acttctttct actaaccgct aaacacggta ttaaaaatta tgatattgat atctatgcca
     2101 ataatgaaag ccaagcaaag actagttttg atgatgtatt taaagtaatt aaagatcatc
     2161 ctgatttaga taaaaaagta tttaaagcta cgaaggaagt tattcaaaat atcgctacaa
     2221 acagcaaact tcgttataac acggcaaatg ctagaacaaa agatgggaag cgaccaggtg
     2281 caaaccgctt tgatgaaatt cacgaaaatg aagattattc aatgataaat gtggctactt
     2341 ctggtggtgg taaaattcga gattatagag aattttatga tacaactaat ggtcatgttc
     2401 gtggtggtcc gcttgatgac attatagaag aatcaaaaat gattctttct ggagaacttg
     2461 gaattgacaa ggatggagca gaattttcta gtttgtttcc atttatttgt cgcttggata
     2521 acgataatga agttgatgat cccgacatgt gggaaaaagc ttgtccaact attaattaca
     2581 atgcagattt aaaacggaaa atgtttcaag aatactctca aatgcaacgt aatgctggtt
     2641 taagacttac gttcatgacc aaacgaatga acagacctat ggaagataca cgatttgctg
     2701 ttgcttcata tgatgatgtt ctgcatacga aagaaaaaga atttcctgaa aaaatggatg
     2761 aagtgatagg aacagtcgat tttgctgata gacgagattt tgccagcgtt gggttgctag
     2821 gaaaatacga taaagatgtt tattttacac aacatacttt tatccacgaa tcagcccttc
     2881 gattacaaaa catcaaacga gaggttatag atatttctat agatcaagga aaatcacaga
     2941 tcgttcatgg aaaaaatata gaagctgatt atattgtagg ttggtttctt gaaatgagta
     3001 ataaatatta tattaaaaaa atcgctatgg atatgtaccg tgcaaaaata ttgaagcccg
     3061 ctttagaaga agcaggtttt actgtggaaa ttgttcgaag cggatctgtt acacatggta
     3121 tgttaaaaga tctggttgat gaccttttta ttaatcaacg tttatttttt ggtgacgatg
     3181 cgattatgcg ttggtattgc atgaatgtat atgaagagca tatttctaat ggaaatatac
     3241 gctatgaaaa aatagaacct gaaactagaa aaacggatgg ctttttttca ttccttcatg
     3301 gtttgaattt tttagatgat atttatgatt ctgctcctgt aacagtcaca aatagctcag
     3361 tagaaaatac aggaactgga tttactcctc tagtattcta acttgaaagg aggtgagaaa
     3421 gtggggattt ttcaaaaggc ggtaggatac ttcacaaaaa aagcaacggt tcctttagaa
     3481 gaatactttt gtaaattgca agttgatttt gtgtatcgaa aatttgcgat tgaaacttgt
     3541 attgatttga ttgcaaatgc gatgagtaaa gcggaattca agtcatatga agatggaaaa
     3601 aataaaaaga atgatcttta ctataggctg aatgtagctc ctaataagaa aaataatgca
     3661 acagaattta gaaaaaaact gatcaggaga ttaatattct acaatgaagt attgatcgtt
     3721 tctccgtcta ataattctag cgaaatattt attgcggata gttgggatgt cacagaatat
     3781 gcattgaaag atgatgtgtt ttctcaagtg caaattaaca acatagtcct tgatagagaa
     3841 tttctagaaa gtgatgttat ctatataaaa tacgcagatc aacaaattag gcaactagtc
     3901 gatgcgtatt atcaagcgta tgggaaactc atttctagtg ccatgaatgt ttacaagcgc
     3961 tctaacgctc gtagatacgt actgaaaggg aatttattcc gaccgcaaga caatacaaca
     4021 caagatcaaa tcaataaaat gatgacatca caatttaagg cttttatgga agctgataat
     4081 gcaggtgcgg tatttcaatt acaaaatgag tacacattag aagatttcag cggaaacttc
     4141 caaagcaatt caagagatat aaaaaactta atagacgaca tctttgagat gacagcagca
     4201 gcgtttcacg ttccgaaaaa cctactaaag ggagacatga gtgggttatc ggatcaagtg
     4261 gacgcttttt taatgttcga aatcataccg attgctgaac ttattcagga tgcgtttaac
     4321 gctagtctct atgaagtaga agaatacttg tcagggaatt ttgtacgtgt ggatacaact
     4381 atgatcaaga ttactagctt caaagatttg gttgacgcta ttgatgtagg cattagaaat
     4441 ggagtattta caatcaacga aggaagagaa cgcgttggaa atgatcgctc tgataaggcg
     4501 atggcagatg aaatatttat aactaaaaac aatcaacaag tatcgaaagg aggtgaggcg
     4561 aatgacgaca atgaaaacat ttctagcagt aaagaatgaa ggcgcagtac cgcaaatttt
     4621 tattcaggga tttattggtt ctagttggtt ctttgaaggg aatactgaca agggaatcaa
     4681 aaatattttg gatagtctag gtgatcaaga agaaattgaa gtagtaatta attcaaacgg
     4741 tggagacgta tttcaaggga ttgctattgg gaacttactt aagtcaaata aagcaaaagt
     4801 taacgttgtg attaacggct tagccgctag tgctgcttca attatcgcaa tggctggcga
     4861 tactataaaa atctacaaca atgcacaatt gatgattcac cgcgcttcca catacggaga
     4921 aggaaatgtc gatgacttcc gtacgattgc tgaccaactg gaatcaattg ataaatcggt
     4981 aaaggcttca tataaaacac gattcaatgg cacagatgaa gcattgcaag aacttcttga
     5041 aaaagaatcg tttatggatg cagaaacagc tttgagttat ggattggtcg atgaaattat
     5101 cgatgcagaa aatagctcag gtactgaagc taaaaaagaa caaagcgttg aagaaatttt
     5161 gaatgacgtt aaagaaaaaa gagcagaaaa aattgctgca tttacagcag cattaaataa
     5221 aacatttgga caaggagatg taaaataatg acagttaaaa atttaaaagg tgtaacagct
     5281 gcaagcgacc aattgatgaa agcttttaaa gatggtaacg aagaatcttt tagcgcagct
     5341 atggtaagct tatctaagga aattcaggat aaaattttag aagaagcaac agcaaaaaat
     5401 caagatcaat tagtattaat gaaccgtggt cagcgtgtat taactacaca agaaacaaaa
     5461 ttctataacg aagtagtgaa aaacgaaggt tttgcagggg tcgaagaatt agtgccagct
     5521 actgtatttg aacgtgtatt tgaagattta gaacaatctc atccactatt gcaaaaaatt
     5581 acttttgtta acacaactgg tgtaacagaa tggattgtgt cacgtggagt caatccagca
     5641 tggtggggta aactttgcga agctgttaaa aaagttttag ataatgactt tgatgtaatt
     5701 aacatgaagc agttcaagct atcaggttat attcctgtat gtaaggcaat gcttgattta
     5761 ggtccagtat ggttagatcg ttatgtccgt actgttttag tagaatcatt gagaattgca
     5821 ttagaacaag caattgttga tggtactggt aaagatatgc cagtcggaat gatgcgtgac
     5881 atgagcaaac aaactagcgg agaatatgct gaaaaaaaag cagaacctat tacagcttta
     5941 gatgctgtaa ctatgggcgg tttgatggcg cgactatcaa aattcaatat cgaaggtgtg
     6001 aatgatccga tttatcgtaa tgtgaatcct tctgatgtgg tcctaattgt gaatccaact
     6061 gattactggt ctaaagtatt cccagctaag actgtactaa ctgctaatgg agaatacgta
     6121 caagtattgc cagtaccagt ttcagatttg cagtcaacgg ctgtgccaga aggaaaagca
     6181 gttattgggg tagcctcaga ttacttcatg ggtgtaggat ctacactaaa aattgaagct
     6241 tcagatgaat accattttgt tgaagacgaa cgcatttatc tagctaaaca atatgcaaac
     6301 ggtcaaccta aacgtaacga tagtttcatt gtattagata ttagcgcttt gggaactact
     6361 actacaacta caaaaccaac aaccacaaca actacaacac aagcgtaggt gatcagaatg
     6421 aagtatattc tttgtcagcc ggcaatcaat cggtttaaat gggagcttga agtttgttta
     6481 actaatctga agaaactagg aatcaaagat atcgtattgc ttttcagcag acacgatgat
     6541 cagattccta ttttttttga gaaggaatat ggtgttgaag ttcatgtgta cgatgatctg
     6601 cgggacgaca aagagtatat tccttcgatt aaaccatatt tatggtggaa atatttagaa
     6661 gaagatcatt cgcgtgagga cgaccgatat ttctatatcg attcgaatgt cattttcaat
     6721 aaaagaatta atttgcgcaa attgccttct aaagatgatg tttggtattg tagcgactgc
     6781 tgtagttatc taagtcttga ttatattaga agctgtgaaa acggagaaaa tattctaaaa
     6841 gatatggcaa acattgtaaa tgttacagta gaatctttgg aaactataaa cactaattca
     6901 ggaggcgcac agtgggttat taaccgtcct aaagcgaatt attggaaaaa ggtttatctg
     6961 gattctaatc ggctatatcg ctaccttaga gggcaaaaaa caaatataca aatctggaca
     7021 gccgagatgt gggcacagct ttggaacatg atgtatttca atattggtcc taaagttcac
     7081 gaggaattag acttttgttt tgctactgat ccaatagaaa aagttaaaga agtaaaaatc
     7141 ttacacaatg ctggagtaac aacaaacgat gaagatttat ttttcaaagg gagatacgtg
     7201 acttctacgc cttttgatga agatttatca tttgtaaaca agaaaaaatg ctcttacgca
     7261 tatgttaaag caattaaggc ggtggttaga tgacgcctga acaagtgact gaagaattgc
     7321 taatagctgt gaaggataat atttacgtta cctggaacga agaagatgag tcaattaaaa
     7381 agatgatagc taaaaatgct gtttatcttc aaagtaaagt gagtacaaca ctttcttttt
     7441 ctcctgaaag cttagaatac ggattgctaa tcgaaagatg tagatacgac tggaatcgtg
     7501 ctttagatga gtttgaacaa aatttcgcta gtgagttatt aggtttcatt caacattatg
     7561 cgctacaaga atatattgca ggtgatgtga atggcgaata atcgtagact cgaagaaaca
     7621 ttcaacgatg gttggttaaa gattttgacg caaactacca aaagaaatga actaggaaaa
     7681 aagattggtg tagaagatac agaaatcact tctttaaaat ttagaaatct ttccatgaga
     7741 gatagtgata taacagctat ggatgcgatg ggatcgaaat taactaagaa agtaaagact
     7801 ccatttcatc caatcgccaa gaaatttaat aaagatcaat attttatcgt aatcgatagt
     7861 atgcgttaca acgttatcta tgccgattac gataattttt atatctattt ttatcttgaa
     7921 agtgtgggtg aatatggtga ttgataattc taaagaaaaa gaacgtttaa ataagcaaat
     7981 ttctgctatc aaaacttcct tagaagagca ttttggcctc aaactctttc aagactccgt
     8041 aggcgaggat gagctacctg atgattttaa ttacttcatt ctcgaaacag gagaaataga
     8101 aatgatcact gagccaaaat atagcgtggg tcaaaatcta tatctaactt tctattcaga
     8161 aaatagagaa gatttaacag gagactcact agatattatt tcattgattc aaaatcgttc
     8221 gattcgtttt cagagaatgg atcccaatca tttaaaacta gagaaccaag atcgctatat
     8281 cgatcaattg gtatttacgt ttagacgatt attgaagagt gattgtcatg gctaaaaata
     8341 gttgggagct aaaaataaat ggacatgatg aacttcttgt gcggatggaa cgctattcaa
     8401 gcgagagcga acgactgatt aacgaagcat tgaaatcaaa aggttcggct attgcagtgg
     8461 ataggattac agaaaaaatt cctgtttctg aagcagattt aagaagaggg caccaacacg
     8521 caaaaaatag tcgtccactt aagactcaat acattaattt gggtttcatc attagaccta
     8581 caagaaaatt tgagtattta aaatatcctg atttggggat aggtacttct aaaagaaatc
     8641 agccggacga atttatgaga agaggattag gtcttgcact tgatccaatt acagaacttc
     8701 tgattcgtca attcgataaa ttaaataaat agggggaaca acaatggcta aaacaaaaac
     8761 tgtagtaaca acgttcgata acataagtat caaacgaatt gcttttaatt ttaagaacgc
     8821 aggaaatgca atcgtaacga attgtaatgg acaattagat ggcgaaacag aaatgcaaac
     8881 ggtggttaaa aaatgtggag cgacagaagt aaaatcaaaa tctaaaccaa tcaatatgac
     8941 ggtaacaatt actgcacatg taccgatgga agtttatcga cgtttcaatg ggttgaaaca
     9001 agatggacgt attaaaccag gcatttactc ttacggtcct gattccgtag gcgaagattt
     9061 ctcacttgct gcagagatcg tggatgactt cgaagaaaat agaaagttag ttggtatgtt
     9121 agcatgcact tcgaatacag gattaacatt ctctattgaa aatggtgcgg atgaagtagc
     9181 tgcgttagaa ctagaaacaa aagttatgca agatgaattt ggtaaatttt atcatgaagc
     9241 aattgttgca gaacttgaag aagacttaac agatcaatgg atgacgaatc tatctgctga
     9301 tgtgattaaa aaaacttcag ttgtgacaac tacggccact caatcacagt aaaaaaaacg
     9361 gaggtagcga aatgaacgaa gattactcaa aaattgaact aaacgatgga acaattttga
     9421 atttagaacc taaactgaat atcaagaaat tattgatgat caatagagat tttaacacag
     9481 acgagtttgc aaaaatgtcg atgggaaaag gctctatgga tattacagtt attcaaggtg
     9541 caaaagccgt atatgtagct tatcgtcaag cgaacatggt cgattacatt tcattcgatg
     9601 aatttataga taaatgggat tttgatatgg aggttgcagt agctgtatac agtactatga
     9661 tgttcaaaca agcacgtgat gcctatcaaa aagaattcga aaaagcaaat aaggaaaaaa
     9721 agcttcaaaa gtaaaaatgc caaagctctt agttgaaacg tgggtcgatg tctattcgat
     9781 gttgaccgac gttttttcta tgccttcaga tttggtttta agcgatatct gtttagatga
     9841 cattttgcaa atggcttaca acaagagcgc ttatgaagga tggaagaact atgcaataaa
     9901 tcaatcccag aaaaactaaa ggaaaggagg taaaaaatgg ctaaaaagag aacggaagca
     9961 gaagtaactt tcatagctaa cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct
    10021 gaattaacta aaaatagagc agaattaaaa ctagaacaag ctcaattaca acagactggt
    10081 tctgaatcag acaagttagg aagtaaatta tcttctttag aaaagcagta tgaattacaa
    10141 agtcaaaaag ttgaagtaac tagtcaacgt ttagccaatg ccaaaaaata ttatggagaa
    10201 aattccaccg aagttcagaa acttgaaaga gaactgatta atcaacaaac agctcagcaa
    10261 aggttatcta atgatataga taaaacgagt aaggcattgg ctcaatctaa aggtgaattt
    10321 aaaacgtatg agtcaactat gaaagactta gataatgagc aacaacatct aaagtcaagc
    10381 gcatcattag tagaatccga atataaaaaa tggcaagcaa ctgctggcca gtctgctaca
    10441 gaatctgaaa aattagcaaa agcacaagaa tatgtcggca aacagagtga tattgctgaa
    10501 caaaaaatcg aagtgctaaa aaaacaatta gatgccacac aaaaagaatt tggagaaaca
    10561 tctaccgaag ctttagaaat gaaaactaag cttaatgatg cggaaagaga attcgaagaa
    10621 ttaagtaatg ctgctaaaaa cgttgatacc tccacagtag atgatatcgg caaaaaactt
    10681 gatatgggta atttaatgga agctactgat cacttgtcag tgatcggaga taagcttatt
    10741 gatgtaggta gtaagtctat tgaagcagct ggaaaagcac aagctatgca ggcccaattt
    10801 aaacaagtct ttggctcttt agaaggggaa gcacaggacg ccgttgaggg aatggctgaa
    10861 gaatttggaa tgttaccaaa tacgatcaag cctgttttta tacaatatac gtcaatgttt
    10921 aaaggacttg gatatgatac caaagaagct atggagttag ctggtgatag tactcagtta
    10981 gcagcagatg cagcagcttt ttacgataag tctatggatg atgctagtga atctcttaat
    11041 tcatttataa aagggaacta cgaaggtggg gagcaaatag gtttatttgc taatgatact
    11101 caaatggcag cttatgctgt taagcataat ttgataccag cgactgaagg agcaaaaaaa
    11161 gccagtgaag agtcattgtt agctgttgaa aaagcacaat ctaagtatgc tgatgctatt
    11221 aagaaacatg gtgaaggatc tttagaagca agagaggctg ctttaaaact taaagatgcg
    11281 caagataaaa taaatgaaga attaggccca caaacgcaaa aatggtctga tttagatgag
    11341 gctaccaagc aagcagttcg agttcaatat gctgaagata tgcaaaaatt agccggtgct
    11401 acaggtcagg ctagtaggga gtctgatggt ttagaaaacc aaatgacaag agcaaagcaa
    11461 gcgttagaag atttttatgc ctcattgggt gaagatatac tgcctgtgtt tatcaaaggg
    11521 ttgcaagcag gagcgaaagc tttgcaagga ttagccgaat ggtggagtaa acttgatggg
    11581 ccaatgaaaa atttcatttt agctcttgga ggaattctag cattattaag cacattagct
    11641 cctgttataa ccgcagttgt tacgatagtt ggcacatttg gttctacagt tttgctgcca
    11701 ataataggaa ttatagcggg cgttgcagct gtaatagcga ttgtaattac agcgtttcaa
    11761 aactggggcg caatcactga ctggtttagt gatttatgga aaaaatttac cgattggtta
    11821 ggtgatacct gggaaagtat aaaagaaagt gcctcatcag tttgggatgg agtcaaagaa
    11881 acctggtctg gatttgtaga ttgggttcaa gaaatttggc aaggagtttc tgattggttt
    11941 ggagagttat ggagcggatt agttgaagga gcttccaaca tctggcaagg agtccaagag
    12001 acttggcaag cattcgttga ttgggtttca aatatttgga acggagtcaa agaagtatgg
    12061 tcgattattt gggcagacat tgtaggaatt gttcaaatac catggacatt aataacgtca
    12121 ttgattcaag ctggtattaa tattatcgtg ggtatctttg atgtagctgg acagttatta
    12181 ggcgcagctt ggcaagctgt ttggacacct atttctgatt tccttaaaaa tacttgggat
    12241 actatgacac aatggataag catcgcttgg aatggaattg taactacatt ccatactata
    12301 tttgatccag tagtggcatg gtggaatggt atatggacat ctattagtac tacggcttca
    12361 aatatttgga attcaattag tgcaacagct tctagtattt ggaacagtat caagaataca
    12421 atcactagct tggtacaagc agctgctaca gtaattcaaa atatttggtc aactgtatct
    12481 agttggttag gtggaatttg gaattcaatc agctctacag catcaaatat ctggaatagt
    12541 gtgactagta gtataagcaa tgctataaac gcagctaaaa gtgccattca aagtgtttgg
    12601 aatagtatat cttcgtggat cagcggaatt tggaacggta tcaaaaatac tgctttgaat
    12661 ctttggaatg gaattacaag cactattagc tctaaagtaa acgatggaaa aaatgcaatt
    12721 tcaagcggtt ggtccaatct aacaggtatt gtttccgaca tattcaataa tgttaaaagt
    12781 acaattgcta acatatggga aggcatcaaa aagactgtta gcgctccaat tgattggatt
    12841 agagacaaaa tcagtggcat ttttgataat ttgaatattt ctataccaca tattccgtta
    12901 ccggaattta tcatggaagg cagctttaac ccgctaaaag gtcaaatccc ccatttgcgt
    12961 gtaaaatggc atgctaaagg aggtatcttt actaaaccaa ctttactagg tggaatgaac
    13021 ggtgtcggtg aagcaggacc tgaagcagtt ttacctttga aaagatctgt tttgcaagaa
    13081 attggtgatc gtatcttgag tagcacatca gtttcatcta gggcacaaac gattcaacct
    13141 gtgaataact acgaattcaa tttcacaatt gatggtaacg cagatgaggt tactatgaag
    13201 caaacaactc aacaaatcat tgatagcatt acaaaaattc aaaatgataa tgcttcggca
    13261 tggcgttaaa caggagagta tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa
    13321 tgactgattg tatacattct ataatcgatg gatttcctga ttatttgcat aaattggctt
    13381 tagctgaaag accaaccata ccttctccaa aaagacagag agttgaaact tctgttttag
    13441 gtaggttagg tggcttagta caagattact cgtttgaaga tatgtcgttt acattgcact
    13501 ataactattt agaggatgtg gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc
    13561 gtcattggtt aaactatgca aagaaattag aattctctga cgatcccaac gtctattacg
    13621 ttatccagac tatcgatatt ggggatgcag aaaacgatat tgttgaatgg ggagagttcg
    13681 atgtaaatat tactgcgaaa ccattcgcaa gagttcaaga agatgtacct ataaccgtag
    13741 ataaaccaca gtcatttaac ttgctgaata atagtttaga agaaagtttt ccaaagatta
    13801 tcatcactcc ttcagctact tcatgccagt tcatcttaaa tgattatgtg tttagtttcg
    13861 aaggcttagt agcaggaact gatgtagtca ttgatagtga tttgatgctt tgctacgaag
    13921 agcaatcgga cggagatatt ttagatcggt ccaacaaaat gaagaccatg caatatccaa
    13981 cgttgcaagt ggatattaat cattttaatt gtactggttt gagcaaaata caaatttatc
    14041 gtaacgggtt aaggtaggtg aaatagatga tcgataattt aataactatt tacgataaaa
    14101 atgacgcgaa taatttagct gaacatttat atgatacgca aggtttaggc gctttgtcag
    14161 actggttaac agctactgtt agcaataaac taaacggagc cgagatattt cagggtactt
    14221 attcaataaa cggaactaat gcagatttga ttgtagaagg acgtattatt cagtgttatg
    14281 tagatgaaaa tcgagcaaaa cagcgtctac ggatttatta tgcaaaaact tctgtaatag
    14341 gcaatacgat agaagtaaaa gctgaaccta ttttcaatga tataagaaaa tcggtgttga
    14401 ataaatatga cagcggaaca gaaaagatca ctgctactca ggcatggcaa aacgcaaaag
    14461 ttttagcgaa accagctatc ccttcgcagt tttctttctc gtcattagta gatacgcttg
    14521 ctaatgtgaa gatagaaaag gcgaattttt tagaattctt tggtggaaaa gagggatcta
    14581 ttctagatcg atttcatggt gagtttctaa aagataataa cacattacgt catgaaaaaa
    14641 gtctaggtac ggatcataaa atcaaagcga tttatactaa aaacttaact ggtcttgact
    14701 tagagattga tgctcaaagt gttttagttg gagtttatcc attcattagc agctcttcag
    14761 aaggagaaga cgagatcact ctaccagaag aagttatttt cacggattac gtggatgatt
    14821 atcctgctgg atatgtttct tttgttgatt ttaaagacaa agcgactgat gtagccacat
    14881 taagggaagc tgctaaagac tggttgaaaa caaacataga taaacaaaaa ccacaagtga
    14941 gtggttcgat tgaattagta ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc
    15001 tagaaaaagt ttcgatgggt gacggagtag atgtgtatca tccacagtta aaagtgaata
    15061 tgtcagcgag aattgtggaa tatacgttta atgttctaac caattcatac gataaattag
    15121 ttgtaggaaa cgtcaaaaca aacttcttag aaaacacaga gaataatgtc agcaatttga
    15181 ttaatgatgc cattgatcaa ttgaaaaatg gtggcgaaat cagtgattta atcaatgata
    15241 ttgtagatca tcaaactgat atgattactg gtcaaaatgg tggttacgtt ttattagatc
    15301 ctaaagaagc ccctagtcgt attttgatta tggacacacc agataagaat actgcacgga
    15361 acgttttgca aatcaacaac gctggtattg gtttctctaa aactggcatt aatggaacgt
    15421 atgacaccgc atggacgtta gatggcggat tcaatgcctc gtttattaca gctggtgaga
    15481 tagtagggat tactattaga ggtactacat taattagtga tggcactgat tatagaacaa
    15541 gtatagctaa tggcaaaatg acttggtact caaaaaaagt taacaaagat attatggagc
    15601 tagaagcacg tgattatgta agtgctgatg ccggcattgt atcatacacc atgaaaactg
    15661 gtggtggttt catgattaga aatccacagg gtaacttggt ttttagtacg tgggataatg
    15721 gtaataacag accgttttta tcttttggtg cgccaaattt cagatacagt aatgctagtt
    15781 atataaatga tggtgacggt aattctttag gtattgatgg tagtgcaggt aattcatggg
    15841 aatttaaagt agctggtagg actatgaaat ttactagtga cggtatgcta acgttgccag
    15901 gttgtttttt tggttcatgg gaagatggga aacttgctag atttgaacaa tcaacggtac
    15961 aagtatataa agattttact gttagaggta ctaaaaactc aaccgtacca acagaacatt
    16021 atggacaacg actattgaac gcttatgaaa ctccagaata ttatttcgct gattatgggg
    16081 aagccgttac aggtgatgat ggtaaagttc gtgttgatat tgaccccatg tttgctgaga
    16141 cagtaaatct aagtcggtat atgacacatg tgacacctac agaactagtt ttgtgtgctg
    16201 ttactcatga agatgttgac catttcatca ttgaaactag taagccaaac gtattggtta
    16261 gatggaattt agtggcacat cgactagggt atgaagatgt tagattaaaa gaggatacag
    16321 catatgatag cacagtgctt gaccaaaaac gtttttaaaa cgaagacaag gaggtatata
    16381 aatggctagc agtttatata atttggcttt agatttcagc aaagaattaa actacaccaa
    16441 agctattatg gctcgtcaag gtgataaagg gatcacggtg actgttaaac cgtttttaaa
    16501 tggcttgcag atggatacga gcggcggaac atttacttta aaaggaacta cgccatctaa
    16561 ccgttacgta gatagtgtcg ctactagtgt aactagtgaa gaagtcacat tttctcttga
    16621 tggcacattt atgagtgaag cagggtatta taaacactgc tatgtagaat atagaaaaga
    16681 cgatcaaatt ttaacgacgc aagatatcat ttttttctca ctaggagtgt ctgacatttc
    16741 gcaaggccaa gccgatgaat atgtttcgca attagaagaa ttgattcgaa agtataacga
    16801 aacttttgat gcttttctgg ctgaaattaa aggtagaatg gatagcttaa ataaacagat
    16861 tactgattta actggtcaag ctaaaacact acaagacaag ttagatgctc tgaaagaaga
    16921 aatttctaaa ttaggtaact tacatgtgat gtactccaac agcatcgact tcgggggcta
    16981 tgattatagt gggaatccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg
    17041 ttaccacggg tcactaacta atgttaacgg aatgttacat tttatgtctg atggtacagg
    17101 ggacattgat atgtttacgc gtattaatac acctcagctt gctagtggca aaacatatac
    17161 tctgagtgca aaagttcgat ttgatgaagg aactacagga gctattaata aattacgttt
    17221 ggtatatcgt acatcaccag gagaaaatat attattggaa gcaaataata caactattac
    17281 aatagatgat gtagggaaag aaataacaat caaaggcaca gctaacgtta attatcaaat
    17341 cacaaattta gatcgttttt atattagtgt tagtttttct aacagggata aaataaatgg
    17401 tggatttaaa ttgtacgaca tcaaaatcga agaaggctca acagccactc cgtatcagcc
    17461 aaacttacta gatgaccctt actgggtagg taaaacatcg ttgggtaaga atattgcaaa
    17521 taaaggtacg acgttcccaa ttaaatctag cgcgtacgaa atatataaag gtaacacgga
    17581 agaagagctt ataataggtc aaacgtatac tatcacgctt aaaggaacaa aacccgcaag
    17641 tcaaaccttt gtagcatata attattggaa cgttaatttt ggagatctaa aaccagttga
    17701 gggattgaca gacgtatggt ctctaacatt cacaccaacg aaacttgaac cgggtttgcc
    17761 taaagacctt cgcatttttc agtcgcctaa agaaacggcc ggcgcatgcc gaattgactg
    17821 gctcaagatc gaaaaaggcg acacacgaac cccgaatatt gagcaatata aataccgagg
    17881 aatcggcatg cgagactcaa acaatccaaa agattatgta tgggatctag aacctagcta
    17941 tgttgagaat aacctagcta cagatatcaa ggttgctgaa atcacaggta aagcaaacaa
    18001 ttataccgat gggaaagtat cggagattaa ttcgcagttg actgcttcaa ttaatgaagt
    18061 agacaccaca gctaaggatg ctcaaacaaa agcgaatgct aatgcgactg ctatagatga
    18121 attagacaat aagatcgatg aacgcattaa tgatacagct actaccacat taacagttac
    18181 aaacgggaat accggatcag caaagcttta tcgtgaagga aaaacagttt ctatatattt
    18241 tgtggcttta aacggaaaaa gcagtggtgg aaatgattca acgatactaa caattccaga
    18301 aggctatcgg ccaccaatta gttttgagca actggttggc tc
//
LOCUS       DANKMN010000062        17955 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000062 DANKMN010000000
VERSION     DANKMN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            133..636
                     /locus_tag="PW224_002096"
     CDS             133..636
                     /locus_tag="PW224_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2908765.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="PW224_002097"
     CDS             763..1800
                     /locus_tag="PW224_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908766.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="PW224_002098"
     CDS             1815..3512
                     /locus_tag="PW224_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDL2908767.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="PW224_002099"
     CDS             3505..3966
                     /locus_tag="PW224_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HDL2908768.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="PW224_002100"
     CDS             3979..4839
                     /locus_tag="PW224_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908769.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="PW224_002101"
     CDS             4877..5266
                     /locus_tag="PW224_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2908770.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5250..7700
                     /locus_tag="PW224_002102"
     CDS             5250..7700
                     /locus_tag="PW224_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2908771.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="PW224_002103"
     CDS             7705..9813
                     /locus_tag="PW224_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2908772.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="PW224_002104"
     CDS             9810..10814
                     /locus_tag="PW224_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2908773.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="PW224_002105"
     CDS             10833..11744
                     /locus_tag="PW224_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2908774.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="PW224_002106"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="PW224_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="PW224_002107"
     CDS             complement(12406..12621)
                     /locus_tag="PW224_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908775.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13047)
                     /locus_tag="PW224_002108"
     CDS             complement(12880..13047)
                     /locus_tag="PW224_002108"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2908776.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(13392..14831)
                     /locus_tag="PW224_002109"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="PW224_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="PW224_002110"
     CDS             complement(15379..16050)
                     /locus_tag="PW224_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL2908777.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="PW224_002111"
     CDS             complement(16399..17850)
                     /locus_tag="PW224_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2908778.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT         5848 a         3016 c         3472 g         5619 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc tctctttttt ctctttaata
    17881 gtgttttcta ttaaaaatta tgataaatat tttcaatgaa ttgagtaata cagtatattt
    17941 cttgatttca tttag
//
LOCUS       DANKMN010000063        17913 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000063 DANKMN010000000
VERSION     DANKMN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(10..1136)
                     /locus_tag="PW224_002112"
     CDS             complement(join(10..908,908..1136))
                     /locus_tag="PW224_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2908779.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1194..1568)
                     /locus_tag="PW224_002113"
     CDS             complement(1194..1568)
                     /locus_tag="PW224_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2908780.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYGVSIKS"
     gene            complement(1816..2652)
                     /locus_tag="PW224_002114"
     CDS             complement(1816..2652)
                     /locus_tag="PW224_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL2908781.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(2663..3592)
                     /locus_tag="PW224_002115"
     CDS             complement(2663..3592)
                     /locus_tag="PW224_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL2908782.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            3784..4137
                     /locus_tag="PW224_002116"
     CDS             3784..4137
                     /locus_tag="PW224_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2908783.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(4211..4867)
                     /locus_tag="PW224_002117"
     CDS             complement(4211..4867)
                     /locus_tag="PW224_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2908784.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            5093..7027
                     /locus_tag="PW224_002118"
     CDS             5093..7027
                     /locus_tag="PW224_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2908785.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(7113..8336)
                     /locus_tag="PW224_002119"
     CDS             complement(7113..8336)
                     /locus_tag="PW224_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2908786.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(8330..10228)
                     /locus_tag="PW224_002120"
     CDS             complement(8330..10228)
                     /locus_tag="PW224_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL2908787.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(10222..11340)
                     /locus_tag="PW224_002121"
     CDS             complement(10222..11340)
                     /locus_tag="PW224_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2908788.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(11423..12256)
                     /locus_tag="PW224_002122"
     CDS             complement(11423..12256)
                     /locus_tag="PW224_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2908789.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(12249..13166)
                     /locus_tag="PW224_002123"
     CDS             complement(12249..13166)
                     /locus_tag="PW224_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2908790.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(13393..14517)
                     /locus_tag="PW224_002124"
     CDS             complement(13393..14517)
                     /locus_tag="PW224_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2908791.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            14700..15607
                     /locus_tag="PW224_002125"
                     /pseudo
     CDS             14700..15607
                     /locus_tag="PW224_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            15748..17361
                     /locus_tag="PW224_002126"
     CDS             15748..17361
                     /locus_tag="PW224_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2908792.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5630 a         3472 c         2959 g         5852 t
ORIGIN      
        1 tagagacggt taagccattt tttcttttgc taattcttca aattcctgtg gggttagata
       61 gtgaatcgag ctatggattc gatttcggtt ataaaatcct tcaatgtagc taaatagcgc
      121 tcgatttgct tcttcaaaat ctgagtagct agtcgtgtag acttcttctt ttttcaatga
      181 ggcatggaaa gattcgattc cagcattatc ataaggtgtt ccttttctac tataagaatg
      241 ccttattttg ttggttttaa gccattgttc cacttctctt gctgtgtatt ggctgcccaa
      301 gtcagtgtgt agaatcattc cttctggaat gtgataatgt atttttgctt tattcaatgt
      361 ttgaatgaca caatccacag tcattcgttt tgaaaatgta taactaataa tttttttcgt
      421 gtgtaaatcc atgattgaag ataagtaaca ccaaccattt ttcttagtag gaatgtacgt
      481 aatgtcagct gcccatttct cacagatcgt ttcagtagaa aagtcctgat ttaagagatt
      541 ctcttttgaa atgatcggtt tattagacct ttgaggtcta tattttttaa ctacaatcga
      601 ccttaaattt aattgtttca ttagcttttg gacatgttta agagatactg aaatcccttc
      661 ttttaataaa acttgatgga tctttgtggc accatagcgt tgtttattcg taaaaaaaat
      721 tgttgaaatc ttctttttca acttattatt tctgatttct gtagctgtca acggtttatt
      781 cttataaaaa taatagacac ttctagggat ttctaataac cgacataaca aagaaacagt
      841 gtaatcctta caccattttt caataaattg aacaagcgaa tttaggtttt ccgagagaat
      901 atggctgcgg ctttttttaa gatttcaagt tcctcattta aacgagcatt ttcttttctc
      961 agttcagcta cttcttttcc agtcagtcct gtggactgat caggtaaata taaattcttc
     1021 catttgtaaa tcgttgcttc agataagcca tattctttcg ctaaccctcg aacagaacgt
     1081 ccagtttgat tcagttcaac aatcatttgt ttaaaatttt cttcatatcg ggtcataaaa
     1141 attcatcctc tcgtagataa gtttaattta ttcttgtctc tacgaaaagc tgtctaagat
     1201 ttgatactaa caccataagt gaattctttt atcaaataac cactaggcgt aatctttcga
     1261 ttaataacag ttattgcttt attcggatga gacgttagat aaacaatttt acacttttca
     1321 ttcttttctc taagcatttc gcctagttcg ataccgctat aataggcgtt caaatcgatg
     1381 tctaggaaaa aaatatctga ggaatagatt tcattatttt taatgaattc aacaaattgt
     1441 ataacgttca atccctgaac gatctcaaat ttccaaggac ttaataaaat tctggattct
     1501 tccaaaattt ctactagcat tttttcaaaa accaaactat catccaaaat aaatatacgc
     1561 gttaccataa aaagtacctc cactcgatat tttactatca aaatcatttc atacaacttt
     1621 tcaacataca tgttaggaat aaattgtaat tctcttcaca atcttttcca acacctttga
     1681 aaccgatatc aaatactttc aggatgctgc ttctaaaata aatatcaaac aaatgaatac
     1741 atcatgcaaa aaaagccaga caaccgacgt ccttgcgtgt cagctgtctg agctatgttt
     1801 atttattttg ttcttttaaa aagaacgata cacatactga tttttcggag cagagacttt
     1861 ctcagcgttt gtaacaacta cagatgggat ttcacgcttg aatggttgat aatagtctga
     1921 ttggatggtt ccttctattt tataccattc atcattttta accgttactc cttccggcat
     1981 atgcgtcaac aaaccaaaga ctcctgaatc tgccacacaa tggataatgc cgaatcggaa
     2041 caagaagata tctgtattgc tatcgttcgg tgtttggtaa acaaatcctt catagctgat
     2101 ttttttgcca atgaattcgc tagggtaatt ataaattagc tccataactt ctaaatagtt
     2161 ttcatctgtg atcttgatag tttgcttatc cccatacttt tctttcaact cattcatttg
     2221 gtctaagtag tctgatttgt tgaaataaat actggtatcc ggctttaagt attgtgtctg
     2281 catctccgga tcccctactg attctttact tactgggaaa ttaaatcctt tggcttctac
     2341 gatcgtagtg tcgagactaa ctgttggaaa caacgtcccc acgattactg gcaaagctag
     2401 cagtacataa gcgattccac gttgataagg ttttgacaac ccatggtcat gatcgtgctc
     2461 gtgtgccttt tcttctgctt ttccgttttt gacccacaaa tacagctgaa ctaaagccaa
     2521 aagagcagat aaaatcattg aaagaatagc taagtagcga taatggacat tgatatattg
     2581 atcgagtttg ccagaaactt gcaagtacat catcaattcg aaatacccag ataaaatcaa
     2641 aaaccgcagc atattttccc tcctaaataa ataatgtgaa tccaataacg ctaagggcaa
     2701 tcagtccgat cagcgataaa ataaagcgtc ctgtaaaata tcgtttcatc atcagtagat
     2761 ttttgatatc gaccattggg ccaaagacca aaaaggcagc tacaggtgct gtgccgaaca
     2821 gacttaacaa agaagatcct ataaacgcat ctgcttctga gcaaagagac atcgtaaagg
     2881 ccaataacag catgatgata atggctaaaa tctttgttga gccgagcgtt agcataattc
     2941 tagttggtaa ataagtctgc atcgcagctg cgattaatcc gccaatgatc aaataacgac
     3001 ctgtatcaaa aaattcgtcg attgaatgtg tcaacacatg cccagtttga tggatcagct
     3061 gattttttgg ttcattcttc tcactttgtt cttcttcatg gacatgatga tggtgatttt
     3121 ctgctacttt agacgtgatt ttttctttca atacagcgtc tttttgaaaa taggcaagcc
     3181 agacaccaat cacaagggca acaaaaaagc tgcctatcac ccgccagctt gccatctgcc
     3241 aagaattgcc aaatgcgata aaagtggaaa aaatcacgat tggattgatg atcggagcag
     3301 tcagcataaa agcaaacgct gtgtgaactg gtacgccttt tttaacaaat tgatgaacga
     3361 tcggtacgat gccgcattca catgaaggaa agaaaaatcc taagatcgaa ccagtaaaaa
     3421 tagagaagat acgattttca ggaagccatc tcttcacacg ttctggggtc aaaaatactt
     3481 gtaaggcgcc tgagataaga cagcctaata atacgaaagg taatgcttca ataacgatcg
     3541 ataagaaaat cgtactcatc tgcaaaaccg aatttggaat aaaagaaaac atgagtttcc
     3601 tcctttttat ttctagttca ttcgctttgc aaatatacgc ttttattcaa aaaaaacaag
     3661 ttgctctgta aatttttaca caactcttat tttttaccaa agcacttcaa ttgacttttt
     3721 ctgttattcc cattaaatta aggtctcata catatcactt ctatcataaa agggagaata
     3781 aaaatgaaac aaaccactaa cttgactgaa gttcaagaca tactgcaaca gtcatccgtt
     3841 gccgtcattt atctttcgat gcctaattgc agtgtttgtc acgctgttcg tccacgacta
     3901 gaggaacttc tcacacatta tgatattccg gcacttcatt tggatgcatt tgaaacacca
     3961 gaagtagcta gtaaatttga agtgctgact gcgccagttg tcctgatttt tcatcaagga
     4021 aaagaagtct ttcggcaagc acggttcatc gactttaaaa aaatccgtta tctactagat
     4081 gaactgacag cagaagatga ttccctttct tatgaagaaa tcttccgaac aaaataatcg
     4141 ttgtccaaac agaaaaaagc gattgaacaa gtgaaagttt tgccttcatt tgttcaatcg
     4201 ctttttattt ttattctgct gtattttcgg aatgcgcatt tgcgtcagaa tgcaagaaat
     4261 agatcaaggt ttgcaattca ttcgtcaaat caacattttg gatcaataca tctgacggag
     4321 cattcaatcg agcagcggta aagttcaaga ttcctttgat tcctgcttcc gttaactgat
     4381 tgactacttc ttgtgctctt tcagcgggta tcgttaaaat tgctacttcg atttgttgga
     4441 tcttgatttg ttctgacata tcttccattg gataaacggg gataccgtct acgatgcgcc
     4501 caacgatttc ctcattcaca tcaaaagcac aactgatacg tatgctatta ctttgatgga
     4561 atttgtattt tagtaaagca cttcccaagt tccctacacc gatcagcgca acatttgtca
     4621 attgatcgtc gtttaatgtc tttgcaaaaa agtgcataag atcttctaca tcataaccat
     4681 acccacgttt tcctaattcg ccaaaataag aaaaatctcg acggatcgtt gcactatcta
     4741 cttgaacggc ttcacttaat tcagttgaag acactttttt ctttccagca ttatttaaaa
     4801 tccttaaata gcgataatac aaaggcagtc ttttagctgt cgcctttgga ataatatgat
     4861 ctttcacgaa atgacctcca cgtattgtct aattacatct ttgatttctc catcatagca
     4921 gtatttatag gaaaaaatca attccattgc tcagatattg tgaaatttta ttctttattc
     4981 atttatttat catttctgca aatctcgcag tataatggaa tttatggtag actactaatt
     5041 gaaaaatagt tttttatttg aggagaattc acaaagtaga aaagaggatt ccatgatatt
     5101 attacaagcc aatcagattg ctcgccattt tggtgcagaa gtcttatttg aaaacataca
     5161 tttagaaatc agtacaggcg ctcgtgtcgc tttagttggc agaaatggcg ctggaaaatc
     5221 gactttatta aagatcattg ctggaattga agcacctgat gaaggaaaga tcgctaaaaa
     5281 taaaacagcg actctcggat acctcgctca ggacacagga cttgattcca atgaaacagt
     5341 ttggaacgag atgctgaaag cttttgaaca agttagacaa atggaagagc ggatgcgcga
     5401 gctagagatt ttcattagcg aaggtacgcc agacacacct gcttatagtt cttatttaaa
     5461 agaatacgac cggctgcaac acgatttttc agatcgaaac ggctatggtt atgaaaatga
     5521 aattcgttcc gtattgcatg ggtttaaatt cgattcttca ttttatgatc agaaaatcga
     5581 tactttatca ggcggacagc gcacacgttt agctttagct cgcatgcttt tgcaacaccc
     5641 tgacattttg attctagatg aaccaacgaa ccatttagat atcgagacac tttcctggct
     5701 tgagaattat ctgcaaagct atactggtgc attattgatt gtttcccatg accgttattt
     5761 tttagataaa gtagtcaatg aagtctatga attaagcaga agaaaaatgc gccattacaa
     5821 aggtaactat tcgaaatatc tagatttgaa agcagaacag ttagcaagtg aatggaaggc
     5881 ttatgaaaag cagcaaacag aaatccataa attagaagac ttcgttgctc ggaaccttgt
     5941 tcgcgcctct accacaaaac gagcgcaaag ccgcagaaag acattagaaa aaatggaccg
     6001 gctggatcgt cctcaagggg atgaaaaaca agcaaacttt ctattcagta tagaaaagcc
     6061 ttctggaaat gtagtcttgc aaatcgaaga tgccgctatc ggatatgata atgtcacctt
     6121 atctgaacca atcaatctag atatccgtcg tcaagaagcg attgctctag taggtccgaa
     6181 tggtatcggg aaatcgacgt tgctgaaatc aatcattcac cagatccctt ttatccgtgg
     6241 aaaagaaacg ctagggacaa atgtcaccat cggctattac gatcaaggtc aagctgatct
     6301 gcatacaaac aagacgatcc tagcagaatt gtgggatgag catcctacca ctccagaaaa
     6361 agagatccga agtgctttag gtgctttctt attttctggc gaagatgtag aaaaaccaat
     6421 ccctctgcta agtggcggtg aaaaagcacg ggttgcttta gccaagttag cgatgaacaa
     6481 agaaaatttt ttgattttgg atgaaccaac aaaccattta gatattgaca acaaagaagt
     6541 attagaaaat gcattgatcg attatgacgg cacactgctt tttgtttccc atgaccgata
     6601 tttcatcaat cggatcgcga caaaagtcgt cgaactctct gaaaatggca gcaaagtcta
     6661 tctaggagac tatgattatt atttagagaa aaagcaggaa gaagaagaaa tcgccgaatt
     6721 gttggcacaa gatgagccag aatctgctcc tgctcccacg aacaaatctg attattacca
     6781 aagcaaagaa cagcaaaagc ttatccgtag ccttcaacga aaaattactc aggttgaaga
     6841 agaaatggcg cggatcgatg aatggatcga tcagttggaa caagaaatgg tcgcgccaga
     6901 aaacctggaa gatcacgtaa aactcaatga gctgaaccaa gatttagaag ctgctagaca
     6961 agatcaagaa acaaaattga cggaatggga agaactaagc ttggaattag aagaatatga
     7021 aaattaacga aaaaaccaaa acagaaacca ttcatgctgt tttggttttt ctttttttat
     7081 tttatcaaac aaaaattcca tatttacctt tttcaccaat aagaagacca attactagtc
     7141 attctcacta aagcttctaa ataatagtaa tcaccccaaa tacaaggtgt atttacccca
     7201 ttatttttgg gcttattata aacagactgt gttaaaaagc catcttcttc ttccgttgta
     7261 gcatatcttt ctttcaattg tcgcataatt ttccaaacag cagactggta taagttcttt
     7321 tctgttttgt ctaatagacc tgattctatt aattctaata atccacaaac catgatcacc
     7381 atactagatg tatctttttc ttctctgcta gaatcatcaa agatcaaatc ccaataacaa
     7441 atggtatctt ctggcaaccg attcataaaa taattggcta atattttgct taaatcaaaa
     7501 tatttttgtt ctttcaaata acgataactt aatgcaaaac cgtaaatccc ccatgcttgg
     7561 cctctagccc aacaggaatt atcactataa ccttgatgcg tacttccata tcttggtttt
     7621 ccatctttga catccatata aaatgtatga taagttgaac catcttctct tactaaataa
     7681 tctgctgcat tttgaatatg agcctgagca gcttttttat atttttgatt tccagtttct
     7741 tcactcgccc aatacaataa tggaagattc atgcaacaat ccatgatcat tctaccttgt
     7801 tgtgaaggat cactaggatc tccccaagct tgtataactc ctgccttctc ccaaaaacgc
     7861 tgaagtaatg tttctgcagc atcaattgaa gcttccttca tttttcgatt tcccgttatt
     7921 ttataatctg ctacggttga taaagaatac aaaaaaccaa gatcgtgatg attagtgata
     7981 atttgacagt caatcctgtg gataaaatct atatctgctt tttcagctgt ttgttgaaat
     8041 aaaggatcac ccgtcttctc aaataacaac caattaattc ctgtccaaaa tccttctgtc
     8101 caaccaatat tgtctcttaa tggataacgt ccttttatag gacactcgct tgggaattga
     8161 tactgactgt tactgatacg tttgattaac ctctccgtgt gtctgcgaca acgctcgatc
     8221 tcattattcc attctttttc cattaattct ttataacatt catttatcca tttttctgga
     8281 tgtattatct cttcaaacat cactttatca agcatttccc caccagcctt tagcataatt
     8341 ttagtctttt tttattgttt ttattttgga tagttagccc ggttgtccca tcaaaatcaa
     8401 ttttgggacg attgttccat acttctaaac tataatcccg atcttgatga gcaaccactc
     8461 ctgttccgag ccaatgggtt ccttttccaa atgtaccctc taatccagga ataaaagtgt
     8521 tttcccatgt catcaagttt gtattgggca aacccataat agctgtggat tttcgtttaa
     8581 tagtatcgtc acttaaacag ataatacctg aaaagccagc ttcactcacc ctatagtttt
     8641 ccttgtcttc ttctatgatt attccttctt tctcaggaga acattcaata gcaaaccctg
     8701 tctcatatag aacatattct gtatctaatt ctactttgtg tacgcgaatc tgccagcctt
     8761 caaaaggaat aagccaagtt ctgatttttg cctgtaaagt aggttcccat gtagaggcaa
     8821 gtacctcttt cttacataca atgtctcgta cattatttcg aacgatgaac tgtccatagg
     8881 ttttgatgct ctcattctta aaactggtcg tgatatatgt gtccgctcgc tgaatcccta
     8941 gcattgaatc cattgccatt tctgctttca attgattcgc tcgcggtatc gaaaaaccaa
     9001 attgactaga ataagcaaat tttaaatatt tttcattggt caatgtatgg aaatttggcc
     9061 ctaattgtcc cgcattcaaa aaaaatgtat gtccattatc atgttgtatg agcaaattgg
     9121 cttctgaaag aagtttagtt ccttcacgaa taggcatagg catttcttta gccgtccaat
     9181 actcatggtc ttcaggtagc gccaacaata aaaaaatctt attcagccat aatggagata
     9241 acgtactatt atagggttca gtcaatgtca attgagggta ggtataacca atattcagga
     9301 tacctttttc atcaaatatt tctttttgca tccaccatct tatattccga ttgactaacc
     9361 ccttgatttc acctaattta aaaggcaaaa gatcggccca caacatagct gaccaaaagc
     9421 ttgaaactgc aaaacgataa atcatgcttc gaccaaaagg cacatttgca ccatcttccg
     9481 caaaaaaatg aatatagtct ctagaaaaat caatagctcg gttcttatat atctggctaa
     9541 tgggcttttc tggttctagt acgctataaa tcaaaccata ataatgaaat gcaaaaggat
     9601 tataataatc ttggcgacct cttgaactgt ctcgatacca gccattttga agataacaat
     9661 tttctatatt atcaaatgct tctttcagtg ctgattcttt aactggataa ccaaattttt
     9721 ggataattgt tccgacaatt atcttaaaaa attgccaatt accatctgca cattcatatt
     9781 caaaaatttg cgagaaccaa tgaataatct ggcttttttc tcctgtatct aattttttcc
     9841 ataataattg ttcatgatga tataacgcta ataacattgc gggcatttct accatacgct
     9901 ggtctctttc atgtatttct ccccaataat cgggatggga aggatctgtt ccagctttta
     9961 ttcctgatag aattaatttc aaccatcgtt catctaattg gtcaattgcg ggccccgctc
    10021 cccataaaac ccgagagaat ccttccattc ctgcaatttt atttccatat cctacccctt
    10081 gatgtatata atcaatttgt gcgccacttg ctcgataaaa cggctggagt ggttgattta
    10141 tctttttaaa taagtcaaag taatcctgtc tggtccttaa tagtggtact ggatgtttca
    10201 ttgtattttc tcctcttttt actaccaaat agctaattcc gactgttcta atcgaagtaa
    10261 gccttcaata aaaaaataat cagaatagat cagtggcaca ttataagtca gttctctatg
    10321 gtatgcttct gctccgcctc caatgatccc atcttcatcc atattccaat ttgcatagtg
    10381 cttttctgtc ttttggagta tattgatagc agcttcatga tacatttttg atcgtacaga
    10441 ggaaacatat cctgctaatt ccagaagacc gcatgcagcg catactccag cactggtatc
    10501 aatattcgaa tcttttacag caaaatcaat gagaggaata taaccagttt tttcgatttt
    10561 ctgaataaaa tttactgcta tcttttctgc tatagctaga tagtacggat tttttgtgta
    10621 acgataactt aacgcgaatc catataaagc ccatgcattt cctctgctcc aacaagaatt
    10681 ctcattgtat ccttgcccgc caagtaaccg aatatatttc cctgattctg catcaaattc
    10741 tgcgatatgc ccggttgatc catctttcct cactagatac ttagctgttg tatctgcatg
    10801 ctgccgagca attttagaga acctcggatc accagtttcc ctcgaagccc aatacagtaa
    10861 tggtaaattc atcatactat caataatcat ccaaccaggt ttatggtcat tccatgctac
    10921 taaatagttc cctgaaatat tatagcgtcc agcaagtacc gtagctgcat gtaaccctag
    10981 ttgtcgacta tgtttgtttt gagactgttg ataatgtgca atagccgatg gtaagaataa
    11041 aaaacctaca tcatgatgaa cgtctgaaaa atggtacagg gcagattcta ataattgttc
    11101 ttgttgtatt gctgcctctt tgaacttttc tcgttttgtc ttgtgaaaga gctgccataa
    11161 aataccactc caaaaaccat tggtccacca gtcgattcct ctgtccttta ttacgtctgt
    11221 atattttccg ttgtaagaaa tatatggaat tttattcccc attctgtcta attccgcatc
    11281 tatcttttca gaagctacat tcaaaatttt tcttgaccat tccttcgtct gttcattcat
    11341 ggatatcccg ccattctttt aatttttatg aatgtggagt tgctcctaga acgaactaga
    11401 tggttaacat tcgttatttt ttttacttat ttgcagctcc aatgtcattc tccttaaatt
    11461 tagactgcgc ccacacataa atcaagacca ttgggatcac tgaagttgct aaaattagca
    11521 taattgcgcc ataattaatt ggctcacctg tactagaaac cgtcatattt ttgataatta
    11581 agggaaccgt atatttatct ttatctgaaa gcatgactaa tggaaataga tattgattcc
    11641 aacttcccat aaagataagt aaaccactag taataatcgt aggtgataaa atcggtacag
    11701 cgattttcgt aaaaatcacc cactcattgg caccatcgat tctggcagcc tccatcagtg
    11761 ctgtaggaaa ttgactgcta tattgtcgca tcataaacac tgaagttgta ctggcaatcg
    11821 aagggatgat cacacctgca taagtattcg ataatcctaa acgactcata atgtaaaata
    11881 aaggaatcaa tgtggtaaaa aaaggcacca tcctagatag cataactgta ttgaacaaca
    11941 gtttattccc cataaaacga tactttgcta atgcataacc cgccattgta gaactggctg
    12001 tagctaaaac tgtactgatt acagcaataa caatcgaatt ccaaataatc aagggataat
    12061 tatatctcgc catgatggct tgccagtttt ttaatagcat atccccaaac ttgaaattga
    12121 ttccgccact taaaatttct tgattacttt gcgttgaagc tacaatcata tataaatatg
    12181 gcatcaaaaa tattaaggca ccaaacacaa taatgacgta cgtaaaaatt ctgctttgta
    12241 tcttttgttt agtcattttt atcacctccg aatttaaatt gaatagccga taatataaca
    12301 gatacaaaca ccatcgtcca ggcaatagca gaggcttttc caaaatcaac catttcaaaa
    12361 cttgttttat ataaattgac tcctacgacc aaagctgaat tttctggtcc attggaagtc
    12421 ccaaagaata tatttgggat attgaaatta cccaagccat tgatagtggc taaaactgtg
    12481 gccattaaaa tgattggctt tagcatgggg atcgttaata aaaagaattg ccgaaatttc
    12541 gatgctccat ctatttcagc actttcgtat atctctttag aaatcccttg aagaccagct
    12601 aaaaataaaa tagtgttata tcctacacta agccaaatca tcgtgatgat cattgatatt
    12661 cgtatccaaa aaggatttgt taaccactgt acaccgttaa ttcctagcgc attcaaaaat
    12721 ccattgaata acccagtcgg attgaaaaaa gtcatgaata cggaagctac agccaccgtt
    12781 gatgtaatgg ttggaatata atagacagtt ctaaaaaatc cttttccttt taactgtgct
    12841 gaattcaata aaacagcaaa gtaaatagct aatagcatcg ttattggaat tgatcctgcc
    12901 atgataatca aaacattcgt gtaagattta ataaacaatg gatctcggaa gatagctaga
    12961 taattgtcaa atccaacaaa ttcaaatgag tagccaattt tcataaaact aaaagcaaac
    13021 gagataatga tgggaatcaa aatcgtcaca ataaagaaaa taacaaaagg tgtgaccaat
    13081 ccataagcac tttttttatg agacgtatct gttgcttttt tcttgctgtt ctttattgtc
    13141 ttgcttggta acgaagattg catcatcgtt aagtcctcct aatatttttt aatcaccagt
    13201 taattttacg ccgtacttat gagcaaaatt ttctgcttct tttagatatg aattttcaaa
    13261 atcacctgtt tgccaatagt tatatgcagc ggtagtcaaa taagacgtag catctgaata
    13321 agtggctggt aaaacagtat ttgatgtatt ttcattgatt ttattcaata gttcatagta
    13381 tttttgtcct ccttagtagt caaacttttt ctcagctgct tctgtttgat aggccaatga
    13441 gttagatgta gccacgccca gctccagtgc gttggctaag cattcttcat ctgtcagcat
    13501 aaatgtaaga aattgttgtg ccacttgtgg attttttgca tctttaggga cataaaaact
    13561 tgatcctcct gatactgcac cttttcctgg attttctttt gaaaaaggaa tcagtggagc
    13621 catgcgccat ttgtcagatg catcaggaaa attcaaagac atattcgtag cataccagcc
    13681 gccagcaaca aacattgcag attcttgaaa tgctttctca gcatcttgtg caccgtagaa
    13741 atgtacaata tctgagtcga tcatttcttt gattaacttt gcagcatgtt tcgcttcttt
    13801 agtattcaga cggatatttc cttcttcatc aagcaaagat tgattctgct gggctagcat
    13861 gttgataaac aattcttggt ccccagattc tggcaaggca attccaaaac taccagtttt
    13921 ttcttttaat gcttttgcca cctggatata ttcttcccaa gaactgattt cttcaaaatc
    13981 tacacctatt tcagcaaatt tttttggttg ataataacaa agtgctgggg taaaatcact
    14041 agcccaagga ataatcgttt cattactcgt ttgttgacgc aaaatattca atttttgatc
    14101 ataaaactga tcgccatatt tgtcataaaa tccatattca tcagcactat aaaaagcatc
    14161 ttgaaatttt tccagcacac ctgctgctga agtgtctgcc atggagataa tatccggcat
    14221 atcttcgtgt gttaccaatt ttggggtgat catttgtact gttgctgttc ccgaagctac
    14281 attcacaatt tttaagtcaa tatttgctcc agttttgcgg ttatagattt ttgcagcatt
    14341 ttcaacatag tttgtcagtt tgccgtttgc gacatatact tctaatgtag tggctttttt
    14401 tatcattgga tctgcgatgt ttgctgttac ttcttcgtta gtttttccac atgcacttaa
    14461 gctgaataat agggtaatgt ttagcaaagc tatcaccaat ttgtatcttt ttttcatgtc
    14521 tatcccttct ttcttttcaa gttcttatat aaaacgcttt cacttatgta tattaacgga
    14581 attagtaaca caaatattta tataaaaaaa tattttactt gtcgttttgg cttttcattt
    14641 ttgttataat agtcattaat tataaataga aagcttttta tataaaaaga gaaaggaaag
    14701 tggttctttt gttaacaaac aaccaatcct ccaatcttgt caaaaaacac taacctattc
    14761 catagatacc catcttcatt tatctgctgc tccctatttt cgactactaa taaatgaaag
    14821 cgctaattta tatgctgaat ttgcacataa atctatagaa ctagataatc cttccctttt
    14881 attcattgat aaacagcaag aagtccaaat cttttctact caaaaggaaa cacaaacttt
    14941 tatcattatc cagttttatt ttgatgagca attattagca catattacgg gacaatcttt
    15001 ttctatagat caagaaataa catctatcat tacttttctg aaaaatgaac agttcaaaat
    15061 tgattatcta aaacatagta agccagctat ttcctatgat caggaaatca attctttttt
    15121 catactatgc caacagatga gcattcattt gctttcctta ttagttactt attgtctgca
    15181 aaaaattcaa ttgtcttcct taccaaggca ttatcaacag gaacttgctt tattgacaat
    15241 accttcaaat gagaaagtca ttcctaaaaa aaagcatatt ctttattcag atgaattagt
    15301 tgatccaatc atacgatatt tagaagaaca tctttcagaa aattttttgc ttgaacaatt
    15361 agcccaaatt ttttttatca gtccagccag cttaaaaaaa cattttagaa aagtgaatgg
    15421 aaaaagtgtt attagctatt ttaaagaact aaaaatggaa aaagcaaaac gtctcgtgct
    15481 agaaaatgaa ttaacctata cagaaattgc agatatatta ggatttcatt cgatccatca
    15541 tttttcttca gcttttaaaa aatatacagg gttatcccct tcaaagtatt atctatcctt
    15601 aaaatagaaa aaaattttta ggttttatcc acaaattatt tctctatatt ttctgtctat
    15661 tcaaaataaa caataatttt ccggcacaat tatttgctgt tttttaccat aacatagtac
    15721 gatcaacgta gaaaaggaga tgtttttatg aaagttgttg tcattggcgg tgtagctggc
    15781 ggtccttcat ttgccactcg tttccgtcga ttgaatgaag cgcacgaaat cattatctat
    15841 gaacgcggag aaaatatttc ttatgcaagt tgtgctttgc cttattattt aggaggtgtg
    15901 atcactgacc gtgactcgct gatcgaacgt acaccagaaa tattgaaaac aaaaaacaac
    15961 atcgatgtct ttactaaaca cgaagtaaca gcaatcgatc cttctactaa gcgattaaca
    16021 gttaaagacc tatccacaaa tgaagaaata aaaacagatt acgataagtt gatcatctct
    16081 tctggtgcta gaccagatta tccggatatt cccggagttt ttgaagcaga aaacggcttt
    16141 gtcctccgta gtgtgacgga tgcggatcga atcaaatcgt tccttgaaga aaaaaatcca
    16201 caacatgtcg tcattcttgg tgcaggtgtt atgggtctgg aattagctga gaatttcaag
    16261 catcgcggct tagacgtgac tttaatcgat caattgccac aagtcgcttt cccttatgat
    16321 ccagaaattg ctaatttagt ttatgacaaa ttgctgaaag aaggattagc cgttcattta
    16381 gaaacaagag ttactgagat ccgtgataaa ggtcgagaaa tagtattatc agatggctcc
    16441 gtcctttctg ctgatatgct aatttttgct gttggtgttt ctccgaagaa tgaagtggtg
    16501 aaagcaaccg gcatacaatt atcagataca ggtcagatca ttgttgatga ccagttacaa
    16561 accaatctgc cggacatcta tgcgattggc gatattatcg aaacgactag tgtagtgact
    16621 ggtcagccga tccaaagtat gctttccagt gcggccaatc gtcaaggaca tatgctggca
    16681 gatattttaa atggtacgcc tatgcgctat cgcggatata ttggtgcagg tgtcgcaaaa
    16741 atctttgatc atacagcaag ttatgcggga atgacagaac atgaattaaa agcatcaggc
    16801 ataacaaatt ataaaactgt ttttatcact ccctttgacc atgcctattt ctatccagaa
    16861 gctacaagat taaatctaaa gctgattttt gatgcagata gcggtcgtat tttaggtgga
    16921 caagcatttg gagaaaaagg tgtcgataaa cggatgggag aactttctgt agcaatcacc
    16981 ggaaacttga cagtctttga tttgcctgat ttggagttgc cttactctcc accgtattct
    17041 actacccgtg acccgttgaa tatagctggt tatgtcgcaa tcaatcaaat gacgaatatc
    17101 gtagaaacga tcaaagcaag tgatataccc gaaaacgatt tgaaagaagc gttcttttta
    17161 gatatacgtg aacctaataa agcaccttct ggcagtatct cggcaacaaa aaatattcca
    17221 atgaatgagt tacgagatcg aatcaatgaa atcccaaaag ataaaaaagt ttacattact
    17281 ttcagaagag gattgaatac ttatacttct gcccgaatct tggcaggttt gggtatcaaa
    17341 gcgattttga ttgaagaata aaaaaacgac cgcggtgggt agccgcggtc taggagttat
    17401 ttttacttga ggagtaaaaa tgaaaaagta tatagggttg ttggttggta tgtatataga
    17461 atagaagata aatgtgaaga aattgtgcat tatttcttca catttattag gttatagttt
    17521 aagaaagttt aaacattgcg caatttaatt attttagctg tttcttttca taaaaaaaca
    17581 tgttgtgact ctgagacaaa tcatagtcca tagtgatgtc tctagagtaa ctacgcctaa
    17641 tattacgagg catcatatta atctatcagc actagtacct aaactagcat ccctttcgaa
    17701 tattttttct tatataataa agtttgcttt cactcctcta atctcaccaa atcgactggc
    17761 ttgcgttgtg tgatactagg aactgattgg tttagaacga cttttctatt gtaatcatat
    17821 ttttcgccat cctcgttgtc ttctaatcct tcaatgaatg atccctaatg tgtgtattaa
    17881 ataatttaat ttttttgttc ctatttattt aac
//
LOCUS       DANKMN010000064        17151 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000064 DANKMN010000000
VERSION     DANKMN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(733..1911)
                     /locus_tag="PW224_002127"
                     /pseudo
     CDS             complement(733..1911)
                     /locus_tag="PW224_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1941..2351
                     /locus_tag="PW224_002128"
     CDS             1941..2351
                     /locus_tag="PW224_002128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908793.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            2432..3052
                     /locus_tag="PW224_002129"
     CDS             2432..3052
                     /locus_tag="PW224_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908794.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            3753..4037
                     /locus_tag="PW224_002130"
     CDS             3753..4037
                     /locus_tag="PW224_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908795.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            4040..4957
                     /locus_tag="PW224_002131"
     CDS             4040..4957
                     /locus_tag="PW224_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2908796.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            4950..5732
                     /locus_tag="PW224_002132"
     CDS             4950..5732
                     /locus_tag="PW224_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908797.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            5733..6413
                     /locus_tag="PW224_002133"
     CDS             5733..6413
                     /locus_tag="PW224_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2908798.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            6406..7302
                     /locus_tag="PW224_002134"
     CDS             6406..7302
                     /locus_tag="PW224_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2908799.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            7497..9146
                     /locus_tag="PW224_002135"
     CDS             7497..9146
                     /locus_tag="PW224_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2908800.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            9740..10675
                     /locus_tag="PW224_002136"
     CDS             9740..10675
                     /locus_tag="PW224_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2908801.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            10668..11438
                     /locus_tag="PW224_002137"
     CDS             10668..11438
                     /locus_tag="PW224_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2908802.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            11854..12837
                     /locus_tag="PW224_002138"
     CDS             11854..12837
                     /locus_tag="PW224_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL2908803.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            13284..14321
                     /locus_tag="PW224_002139"
     CDS             13284..14321
                     /locus_tag="PW224_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2908804.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(14410..14733)
                     /locus_tag="PW224_002140"
     CDS             complement(14410..14733)
                     /locus_tag="PW224_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2908805.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(14720..15427)
                     /locus_tag="PW224_002141"
     CDS             complement(14720..15427)
                     /locus_tag="PW224_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2908806.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            15513..15854
                     /locus_tag="PW224_002142"
     CDS             15513..15854
                     /locus_tag="PW224_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDL2908807.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(16025..16873)
                     /locus_tag="PW224_002143"
     CDS             complement(16025..16873)
                     /locus_tag="PW224_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908808.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         5462 a         2916 c         3574 g         5199 t
ORIGIN      
        1 aatgaaaaac gagaaggaag atgactttaa agtagtttag tatgaaggtc gattgataaa
       61 acgatgtgga ttaatgctaa ataatgagta atttttcaat aatacaaaaa ctgtgtacag
      121 tattagaagt agagaaacgc tagcgtcgaa aaaaagatat ttttcgtgaa aagtaatagg
      181 ggctaaaaag aaagtataca atttatttat gtttctaagc atataaaaaa gagcgttgaa
      241 aggaatattt ccccttcaac gcttcttttt tagaagattt taatgaccat ctcaacttca
      301 ttttcaggtt taggtaattc atggaagctt gtgtttatcg tgcttaattg atcaactaac
      361 catgttttac tttttgactc gtagacaaat ctaaaattaa tatcattgtt ctcgtgttga
      421 agaggtttct ccaaataatc tggtgaatat tcatagtcaa cgtaacctgc taatacaaaa
      481 gaaagcagtc cattatttag tgtcagattt aacggatggt cgtaagcttt tattttttta
      541 atgtcatatt gtacatctta tgaagactta atgactactt gtagtcaata gatttaaagt
      601 atttattttc tcatttatgt atcaagaata aatactgata taataaaaat tatatcaatg
      661 ttttgatgaa gtaatttgga gggagcgtca ataattttgt gtaaataact cgtccttctg
      721 caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc
      781 tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg
      841 ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt
      901 cttgaaagat tcgattaaat tagttgagta aatgcttcgg cggataccag gcggaaactc
      961 ataaaaagtc aataaatctt gattttttat cagtgactgc gtcactttag gataagtttt
     1021 ttgctacttc tcaatcatac tccctaaaaa ggtatttgcc tcttcctttg agctagcttg
     1081 ataaacagcc ttaaaatcat cacagatttc ttttcgatct ttgacacgta ctttatgagc
     1141 aatattacgg gaaacgtgaa cacagcaatg ttggtattta gctttaggat aaatctggtg
     1201 aatcgtatct ttcatgcctt ttaagccatc ggtaataaac agtaagacat catgaacccc
     1261 tctggagtaa atgtcctgta acaactcatt ccaaacgtag gtcgattcag tcggagcaat
     1321 cgcataactt agtacttctt tagtgccgtc ttctcgtata ccaatggcaa tatagattgc
     1381 ttctttggag acggtttgcc gttttaaagg aatataagta gcatccatga aaatagcgac
     1441 atacttatca tttagagttc taactttaaa tgcatttact tcttcagtca gcactttagt
     1501 catgttggac atggtttggg gcgtgtagtg atgcccatac attttctcaa tcaagtctgc
     1561 gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct ctaagatatc
     1621 attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg
     1681 aatctggaga tgaagttccc cgtactcggt cttgaccgta cggtcatagg agccgttacg
     1741 agaattcccc gtgttaaaac caatgcgatc gtacttttcg taatcgagga aagccgttaa
     1801 ctccgttttg agaagcgtat tgacagcttt ttccaaatgg acacggaata attcattcaa
     1861 atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc
     1921 ctcttttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta
     1981 tttacacaaa atattttaca ctctcgtcaa atataaaaac tagtacgaaa ttaatttgta
     2041 aattatttaa gcttgataaa ataagcaata tcgatgatgt aaagtccaag atattaacaa
     2101 gaaaagtaag cgaaccagta aagctaacgg cacaagataa aaaaaatgga attgttcttt
     2161 atggtgatga tgcaaagtat aactataatt gggatacctc ctcagaagct gaggtattaa
     2221 gtattcctac taccgatact tcaggggaaa aagtcacctt taaaataaat tttataacgg
     2281 caatttctaa gttggagtat gggaagcaaa aaaagagaga taacaatgat caatatctac
     2341 ctaaaaaata acgaattaat gatgcatatg ttaaagttgt ttttttgaat taaacaactc
     2401 aatttattca gttatatgtt catcgcagca agtgaacatt aatcgagtta taaaattaat
     2461 aggagctaaa aatataaata aagttcgatc agaatttatt tttccagaag atttttttaa
     2521 ctggtttttt tatcaattca gtgaatgcaa gcgactctta ggtgagaatt attacttaaa
     2581 aggaatcggc ggatttatgg ggaatgtggc tgatgaacaa aatattataa aaggaatatc
     2641 tgaacaaaca tcagagctta cagtaactaa agcatttata agtaatggtg aaagtttgaa
     2701 aaatgtaact gcgcgaatta gaaaagaaaa tgaagtagac attgtttttg gaattgacca
     2761 tgaatgtaat tcagtgatat atgtaacaca atcagcaaat ttaattcttt ttgagaatgg
     2821 ttgcaaggac gattttatgg tggtctattt gtacgatata ctaattccaa aacttcgttc
     2881 cttatatatg tcttctgaaa gtatttttgt gagtgaacaa aagggatctt tctcgaaaaa
     2941 aataggtatt gaagtaattg aatcgattat gtgtcataat catattacgc tagaagaatt
     3001 agaaattttg atagggcaaa aaaataataa cacgaaaata caagtttctt agaaatcatt
     3061 ttttattgag atatctagat aaatgattta ttcttcaatc aaccaactcg ccaagctgag
     3121 gtaatgtttt gaggtaatga taacatacaa ctttttatac cctagtaaaa ctctataaac
     3181 aaagacttat taatttcgca tgaaatacat tcctgtaaaa tgcaataaaa tagagttttg
     3241 tttaaccagg tcttaagaaa gcccgtaaag cttcacagtt ctcaaaacgt taattgcgat
     3301 tatcgttcga gttcacgagt tacgagaatc gtactgggcg aagcaatgct gagcgcagtt
     3361 accttaatcc taacttttct ggcactttcc acaatttgga aagtgccttt tttgtgtttt
     3421 tagctatgtt aataggtagg attcttggtc tataatatca ttcgatctat aaacaaacgg
     3481 gaaaaagata aagtatcatc aaacaatatt atttatccgc ttctgaggtt gtgacagaag
     3541 tggtcagctt caagaaataa gaaggaattt acaaaaattg cttctcatgt ttgataaata
     3601 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcggaag aagggattgt
     3661 gaaaagtctt ttgtcataac ctcttatttg gtagctaatt gtaaatccca aacggcatgt
     3721 attatttttc ataagtccgt ttttaaggat taatgttttc ttttgctttt tatatggtct
     3781 ggcttcagcg agaagttcca acgcatatac aaggacgtct gatgtcaacg ataacaacga
     3841 ttaccatggt ggccagtcca cttggattag cactgtttgg tgcgctcttt gattggcaga
     3901 caactcaacc acgtctgcga gacagtctga tttttcttga aattcaattg gaaaatttac
     3961 actttcttta tacatttctg gctgctttct ttatacccgt caactatact tcacttatca
     4021 ataagaaaga aggataagta tggattttgg aaatgaaacg gtcttgaaag caactggtat
     4081 aaccaaaaaa tacggtgctg ccaaagcatt agataaagta tcgatcgaaa tcaaacgtgg
     4141 aatgatctat ggtttgattg gggagaatgg agcgggtaag tctaccttta tgagaactat
     4201 tatgggtttg atctcgattg atgaaggttc gatcgaatta tttggaacga cggatttgca
     4261 ggcagcaaga cgacgtatgg gtcaatcgat tgaaacccct gctctttatc cagaattaac
     4321 tgcaagagat aacctacgta tccaagcagc gaacggcggt gtgtcggata gagaaatcga
     4381 agacctgctg aaaatgatgc gattggaaaa tacagggaaa aagaaagcaa agaatttctc
     4441 tttaggtatg cgccagcgtt tagctattgc aaatgcactg atcacgaatc cagaattttt
     4501 gattttggat gaaccgacga atgggatgga tccagcaggg atggcagaaa tgcgagaaat
     4561 catccaacgt ttggtaaaag aacggggcat caccgtcttg ctttctagcc acttgctaga
     4621 tgaattgtca cagatcgcca ctcactacgg tattttgcat gaaggacact tgatcaaaga
     4681 attatcgaaa gaagaattgg cacaagaatc tcgtcaattt ataaaaattg acacttcggc
     4741 gacggaacaa gcagtaacgg ttctcgactc tcttggttat agggattatt ttgtccaatc
     4801 tagccgtgtt atccagcttt ttgaaggaat tgaccaagta gcagccatca atcaagcatt
     4861 ggtagaggca aaagttcctg tggatggtat ccacctagtt gggcaaaaac tggaagatta
     4921 tttcttacaa ttaactggag gcaatccaca tgtttaatct attaactgct gaaaaaataa
     4981 aagtatggaa gagccgtaaa atgtgggtct cactaggact gatggtcgta cttccgcttt
     5041 tttattcttg gaacgaatgg tatatgcata caaaatacgg caatgaatta aaccaagcaa
     5101 cggatacagt catcaacggt gcaacaggga ttttaatggt ggaaaaaatg gctgcatgga
     5161 ttcttctggc tttcgcagca tttgcgtgct tttatattgg ggaagaattt caaaacggta
     5221 cgattcgcaa tacgttatct ttaggacgga atcgaatgac ttattatgtg tcgaaacttc
     5281 tcgttacgct tttgatcaca acacttggcg tggttttgat tacggggcta gccatgattg
     5341 cttacacact tgctttcgga tttggtgaag ttgagggaat caaaaactat ggaaattatg
     5401 ttctaaaagt atttcctgta ttgcttctgc tgatattagc gacattatct gttccagtgg
     5461 ctttgacatt catcactaga agtaccagtg tcagtttgct tctttctttt ctttatatca
     5521 tgggaacggc cttcgttccc ggtgttttcg caaaaatcaa aggcttggaa tttctaacgg
     5581 agtggtttac ggaaacgtgg ctcatgtata cggactttgc gcaacaagca acttattcgc
     5641 aagcaccgaa aatgattctg gtcagtctag taacaatcgt ggtatcatca gctctaggaa
     5701 tgtttatttt ccaaaaatct gacattaagt aggtgttcct gctggaaact attttgatta
     5761 tcgaagacga tgaagctgtt cattctttac tggaggaggt tcttgaacag agatacaaac
     5821 ttcttgatgc ttattcaggt acggaaggaa aattattgtt aagcacatat cctgtcgatt
     5881 tgatccttct agatctcatg cttccagggc tttcaggtga agcattgtta gcagaaatcc
     5941 gacagacatc gaatgttccg atcatcgttc tttcagcaaa aagcgatcaa agagataaag
     6001 tttccttgtt ggcagcagga gcggatgact atgtgacgaa accatttgat atcgaagaac
     6061 tgttgttacg aatcggcatc caacttcgtc atcagacttc tgtccaagta aaagatctgt
     6121 cgcaacgcat ctattacaag gaaatcttac tggacaaaga gtcgagggat gtaaatgtga
     6181 atggtgtggc tgtccatttg acaggaagag aattcgatct gctgaagctt tttttagaac
     6241 atccgaaaaa agtattttcg cgagcaaatc tttatgaaac agtttggcaa gaaccatatt
     6301 ttgatagtga gaaaacagtg aatatgcata tttccaatct tcgagggaaa ctagcaaagg
     6361 cacatgctgt gtatattcat acggtgtggg gagtggggtt tagatttgat taagtggctg
     6421 ttgcttctcg ttgttatctt tatgggaagt tttattttct tcctgcaaag ggaaatcaag
     6481 agattaacta agaaaatcgc cactctttct gaacgacaag aatatggtgg tcgtctttca
     6541 gtcgaatttc gagaaaagaa tctaatggac ctagtagatg cactgaatac atgggtggat
     6601 gaaaatgaac aaaaaggaca agcatttcaa gaaatggaag aaaacatcag actatcgatc
     6661 gtcggccttt ctcatgatct cagaactcct ttgactgcta ttaacggtta tgtacaatta
     6721 ctggaacagg tagaggatac ggagaaacgg gagaagtatt taacgatcat caaacaatct
     6781 gttgcaagat tactcagtat gacggatcag ttttatgatc ttgctcgaat agagacgaag
     6841 caaaaagaaa tggatctcca tacgctttct ttttctaaag aggtggaaga agtatttttc
     6901 tctttttatg aacaattttc tgagaaaaat atcgaggtag cgttcgctga acactcgata
     6961 aatacaactg tattagcaga tccgctcatg ttgctgcgag tgctgcaaaa tatcatacag
     7021 aatttattgc gttatgcaga tggacaagca aaggtgacat tttcaaaaga ggattcattc
     7081 attcgattaa cagtggccaa tcctatcaaa ccaggagcgc aaacctctgt tgaaaaagtc
     7141 ttccagcgtt tttataccga aaataccagc cgcacaaata cggaatctag cggaatcggg
     7201 ctttacctgt caaaaaaatt ggtggaagga atgggaggag agatgaccgc tagattagac
     7261 gggggcatct tctccatctc cgtcaagttg cggagggttt aattggctct aatttacctg
     7321 aagtgaatga caaaaaattg tatctcgttt taaagacatc ttcttttttg gaaggtgttt
     7381 ttattttggt gtgattgata cgaattaggt aacgaagaca gttatttttg tacccccttt
     7441 tcaaatagcg caacagtgct atactaaaac taacaaatca aggaacggag agggatatgg
     7501 gccaaagaga atttatcgtt ttaattatta ttgcagttgt tattttatta gttgttttag
     7561 acattttttc acgtttgaaa gtaaaggaaa cagttcgtag taattgggga aaaattcctt
     7621 atcaaccgag atttgataaa gaagaaagtt tgaaggatgc gtggctcacg gagaaaaaat
     7681 tccgttcatg ggatagtgaa atcgacgatc ttacttggta tgacttggat atgttcgaag
     7741 tatttgaagg gatcaacagt acctattcta gtgtaggttc agaagcgttg tatcagcgtt
     7801 tacgtagctt tgattttgga gaagattatc agttagaaaa actaattgct ttttatcagg
     7861 aaaatcctca actgagagaa agaatccaat atcaattcgc tcgtttagga aaaaaagatc
     7921 ataattttgc aaaacaatat ctagcggatg gaaaaagtaa aaagattgga cgcgtaggtt
     7981 tatttatttt tcttggcttg cttccgctgg tgggtgtagg tttgcttctt ttagggcaag
     8041 tactaggaat ctatctagca atcggtggct tagtattcaa tacggtctat tatctagtga
     8101 agaaacaaac actagaaaca gagctgaata gcatgagtta tttggtttca acgatttcag
     8161 ccgccaatca gttagcaaaa atgacgacac cacttcaaaa agagctgaaa cagcaaacta
     8221 aaccattaag agacatccct aaattaggtt tttcatttcg aatgaagagc gggagtgaag
     8281 cagaattgat ttttgaatat gtcaacatca tgttcatgct gccatttatc tcctatcatt
     8341 tcgttctcac taaattagaa aagcattcaa ctgaagccat caagctgtgg gaattgctgg
     8401 gagaattgga agttgcagca gccgttttga attatcgcac ctatatgccg atcacttgcc
     8461 gaccagagtt tactgagggc ggtgtgacag cgaaagatat ctatcatcca ttgttaaaag
     8521 aagcggtagt taatccagtc aactggcaga agaatacatt agtcacgggt tccaatgcat
     8581 caggaaaatc tacgtatgtc aaaagtatag ccatcagctg tattctagca cagacgatcc
     8641 aaacagcgat tgcagaacag tttaccctgc aacctggaca tgtgttgact tccatggcaa
     8701 tcgaagatga tttatttgaa ggcgacagtt attttgtttc agaaatcaaa tcgatcaaac
     8761 gtttgctgga ccaagtggct tccaaagaac gctgctactg cttcgtagat gagattttaa
     8821 aaggaacaaa tacgatcgaa cggattgctt cttccgcttc agtcgttcgg tggcttgcgg
     8881 agtatccatc tttagcattt gtcgccacac atgatatcga attgacagag attttaaaga
     8941 attattgtga gaatgtccac tttgaagaac aagtaacaga aggaaaagga atcagttttg
     9001 atttcaaatt aaaagaagga ccttcaagga cccgaaatgc gattgccttg ctgaaagttt
     9061 tgaattatcc taaatccata gttgaacagg cccaaaaaga aagtttgttg ttcgatgaac
     9121 agcgtcagtg gtatccgttt gattaacttt atcaaacgaa gcatgatgcg tgggtccaaa
     9181 aagacgatta tcaaaagaag tacattgatg ccaaaaaagt ggcagaattc aatgggaacg
     9241 agtctgctgt ccagatgatc ttggaagatt tagcaaaaga cgatacaaaa taaaccacgc
     9301 tgtcttcctt ctagcagctt ttatcgaaaa aacttagcat cagcgacgag acgcacgttc
     9361 catggcaaag ttctccgtaa aacaaacagc gaaaacagaa attgaaaata actatttcca
     9421 ttttatttat ttcggttaaa ataaaagaaa aaagctttcg ataaaaggac ggaggaaagc
     9481 ttaacatgaa taaaaaagac aagaacctca gttataagga tcttctatat gtcatcgtag
     9541 tagttgctgt cggtactttt atgattttaa aagttccaga gttgtttaga tagttattct
     9601 atcgaagatg atcatgaact agaaaacaca tctcctcttt gttagcaggt gtgtttttgt
     9661 gtggaagcca agttgttttt tcatctgctg tattccatga tacaattaag aatcatgtct
     9721 ataaggagat aggaaataaa tggcaaatgc gcttgaaata aataatttga aaaagatcta
     9781 tggaagtggg gtcgaagcat tgcggggaat cgatctttcg gtgaaagaag gagatttcta
     9841 tgcgctatta gggccaaatg gagctgggaa gtctacaacg atcgggatta ttacttcttt
     9901 agtcaataaa acatctggaa aagtgaatgt cttcggttat gatctggaca aggatctagt
     9961 tcgagcaaag caacagattg gattggttcc acaagagttc aattttaacc cttttgaaac
    10021 ggtccagcaa atcgtcgtga atcaagcagg ctactacggt gtctctcgca aagaagcgat
    10081 gaaacgcagc gaaaaatatt tgaagcagtc caatctttgg gaaaagagaa atgtccgtgc
    10141 acgtatgctt tccgggggaa tgaagcgaag actgatgatt gctagggcat tgatgcatga
    10201 accacgtttg ctgatcttgg atgaaccaac agcaggagtt gacatcgagt tgagacgcga
    10261 gatgtgggag tttctacgag aattgaacga aaatggtaca acgatcattt tgacgacgca
    10321 ttatctagaa gaagctgaaa tgctttgtcg caacatcgga atcatccaat cgggtgaact
    10381 gatcgagaat acaagtatga aagagcttct gtcaaaactg caatttgaaa catttatctt
    10441 tgatttagcg ccatatgata ccaaacctgt gattgaagga tatgcttgta catttgaaga
    10501 tgagcagaca atcgctgtcg aagttgaacg aaatcaaggg gtgaacggaa tcttcgatca
    10561 gcttacaaga caaggaatca aagttctttc tatgaggaat aagtcgaatc gtttagaaga
    10621 actgttcttg aaaatcacag aagaaaataa tcatacggag gagaaaaatg tttaatcttt
    10681 attttactgc attaaaaagt ttagctatca aggaaacgaa ccgctatttg cgtatatggg
    10741 tccaaacact ggttccgcca gtgatcacga catccttgta ttttgttatt ttcggaaaaa
    10801 tgatcggtgg gcggatcggt gacatgggtg gtttttcata tatggaattc atcgttcctg
    10861 gtttgattat gatgtctgct atcactagtt cgtatgccaa tgtttcttcg tccttcttct
    10921 ctcaaaaatt tcagaaaaat atcgaagaaa tcttggtagc acctgttcct acgcatgtga
    10981 ttatttgggg ctttgtgatt ggtggtgtgg gtcgcagtat tctcgtcggc tctctcgtaa
    11041 cgatcatttc cttgttcttt gtaccacttc atgtgtattc atggttcatg gtcatcatta
    11101 cattattgat gacggcaatc ttgttttcat tagcaggact gctcaacggg atttttgctc
    11161 agtcttatga tgatgtatcg atcgtgccta catttgtctt gcagccgtta acttatctag
    11221 gtggcgtttt ctatgccatc tctatgttac cgcccttttg gcaagctgtt tcaaaagtca
    11281 atccgatcgt ttatatgatc tccggtttcc gttacggatt ccttggaacg atcgatgtgc
    11341 cagtagcaac atcgatgatc gtacttgtgt tattcatcgt tgttttgtac gccgttactt
    11401 ggtatttgat caataaagga agaggattga gaagttagaa aagaggttgt gaaatcagcg
    11461 ttttgacctg agtattagag aaacaaacgg gaaaatagtt cctcatattt tgttgttttt
    11521 tgagcgaata ccgagggtca gctgattgaa caccgtttag tggagaggtt gtgaaaaagc
    11581 tgttctgcat caagtaataa gaacagccaa acaaaaatag ctcctcctat ttttcgtttg
    11641 gttggactta ttaccgcaga tgcaagcttt tgaacaccat ttattccgga aaatggactg
    11701 tgataagatt tttgtcacag tctttttttt gctcaatcag aaacatcttt taagctgttc
    11761 actttgcttc tttttttgtt tcctagtata atgacaatgt ttttgagaaa gattgcgact
    11821 tcactgcttg atagaatgga gagtaacaaa taaatgacta ctaatttttg gaaagagctg
    11881 ccaaagcctt tttttgtttt agcaccaatg gaagatgtaa cagatgttgt atttcgccat
    11941 gtcgtaaagc atgcagcagc accggatgtc ttctttactg agtttacaaa ctcagacagt
    12001 ttctgtcatc cagatggaaa agacagtgta cgaggaagat taacatttac agaagatgaa
    12061 cagccgatgg ttgcccatat ttggggagac aagccagaat tttttagaga aatgagcatt
    12121 gctatggcag aaatgggctt ccaaggaatc gatatcaaca tgggctgccc agtccctaat
    12181 gtagcagaac gtggaaaagg cagtggcttg atccttcgtc cagaagtagc ggcacaattg
    12241 attgaagcag ctaaagctgg cggtttgcct gtcagcgtca agacgcgtat cggctatacg
    12301 gaaatgtctg agatggaaga atggatcagt cacctacttc atcaagatat agccaatctt
    12361 tctgtccatt tgcgtacgcg taaagagatg agtaaagtag atgcacattg ggaagtcatt
    12421 cctaaaatca ttgctttacg tgaccaaatc gctccacaaa cactaatcac gatcaatgga
    12481 gatatccccg atcgccagac aggagaagaa ttggccgaga aatatggcgt tgatggcatc
    12541 atgattggcc gagggatttt caagaatcct tatgcatttg aaaaagaccc gagggaacat
    12601 acagaaaaag aactgatcgg actacttcgt ctgcaactag atctgcaaga tcattattca
    12661 gaaatcatcc cgcgttccat cacaggacta catcgtttct ttaaaatcta tatcaaagga
    12721 tttccaggag caaatgactt gcgagttcag ttgatgaata cgaaatctac tgatgaagta
    12781 cgagagatct tagagacctt tgaaaaagaa catccaacgc ttttcagtga acaatgattt
    12841 cttgcttgaa caccgtgaat tagaggtcgt gaaaaagctg tcctgtatca agtaataaga
    12901 acagccaaac aaaaatagct cttcatattt tgtcgttttt tgagctaata ccgagggtca
    12961 gttgattgaa caccgtttat atcagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    13021 ggagcaattt caaaaaacag cttctcatgt ttgatgaata ttttgacttg tgaccgaaga
    13081 gttggtagga gaacaccgtt tataagaggt tgtgacaaaa gtcttgtcgc aacctctttt
    13141 ttaaccaaaa aagtgcaaat agtttacttt aagtaagaga ataattatga tgaataaaaa
    13201 aggtggaaca aatagcatac tatatatatt gaaatgaaca ctgtatggat gctccaactt
    13261 gatgaagcta aggaggaatt cgtatgtcag aaaaggaatt aatgaaggta gtcggttttt
    13321 atgaagggtt gccgatcgat gatccaaaaa gctttattga tgaaaaagaa cagatacctg
    13381 tacccagttc acgggatctt ctggtaaaag tcaacgcagt atctgttaat cctgtcgata
    13441 cgaaacttcg ccaagataac gggataagaa atgctttacg tatcttaggt tttgatggag
    13501 tcgggaaagt tgttgctgta ggtgacgaag tgcaaaaatt ttctgtcggc gatcgcgttt
    13561 tttacgcagg tacaacaaca agagcaggaa gcaatcaaga ataccaacta gttgacgaac
    13621 gtattgtcgc tcttgcacca aaaaatcttt cagatgaaga agctgcagcc ttgccgttga
    13681 cctcattgac cgcttatgaa ttgttatttg aaaaatttgg tctaactcca gaagaaaatg
    13741 ccaatcgcgg gaaaaaaatc ttagtgatca acggatccgg gggcgtcggt tcgattttga
    13801 atcaactagc gcattgggct ggcctagaag tctacgcgac tgcaagtccg aagaattttg
    13861 aatggctgaa aaaaacaggt gtagactatc caatcgatta tcatcacgac ttaagtaaaa
    13921 gttttcgtga attaggaatc gatcaagtag agtatgtcgc tgtattattc gatattaccc
    13981 gctactttga tcaaatcaag caccttgtcc gaccatttgg acatgtggga acgatcgtag
    14041 ggatcaaaga gccgttagat ataggtgttt ggaaaaacca atctatcagc ttcgactggg
    14101 agtatatgtt cgctaaaaca gactataact atgaaatcga aacgcaagga gaagctttag
    14161 cgcacattgc tgctttagca aatgaaggga ttatccgcag tacattgagc aaggtctatt
    14221 cagatgggat gaatgcgaag aacttgaaac aagcaaccaa agacgtagaa acaggacata
    14281 tgttaggaaa agtcgttgtt agtggtccgt tcaacggata agcaagaata cgccgatttt
    14341 tttcttattt gactttgata aaaacaagag cgcatgacag taatttgcca taagctcttg
    14401 tttttttatt tataataagc gaatcaacga aagggaaaga attcctgcca agacgatcac
    14461 caataagctt tttgaaagaa tagcagctaa aagtgtagga actgaagcca aagcattttc
    14521 gagattgatc tgcggcagat gtccgatatt ttgggtaaat agactgctga accataaagc
    14581 agacataatg acgatcggga caaaacttaa atactcaagc aacggttgcg gtaaatcgaa
    14641 ttttttcagt aagacaaatg gtaggaccct agaaagccaa gttgctaaag tacaacctaa
    14701 aatagtcagc aatgtaaact caaaagaagg catgtttgat ccacaccccc aatccgcacc
    14761 caatcagtgt tacaactaca atcactaagt tgcttgggat aaaaatcagt ccgacatata
    14821 ccagaatcaa agtgagacca atcaggatca actgtaatcg tttagaggtg tttctatcag
    14881 aaatgacttg aagataaagt aacccgataa acatagcgac gatggcgaaa tccaaaccaa
    14941 actttttcgg atcagtaata aaattcccta aaagtgcgcc aacaagagaa gaacccaccc
    15001 acgtcaaata agaaatccaa ttagctgtat tcatccaacg aaagtttaac ttttgtccag
    15061 tatagttcaa tttattcatt cccagtgcga aactttcatc tgtcagcaat gtgccaatca
    15121 gcagattttg aagcagccga tttttcttga aataaggagc aatcgtcata ctcatcagaa
    15181 tcattcggga attgactaga aaggtcgaga aaacgatgga aagcaatggg ctgtgactag
    15241 caagcatgct gactgtgata aattgggcag aaccagcata gaccagcagg gacatcaacg
    15301 tgacgactag tggatgaaat ccggcagctt tcccaacgat accaaatgca agtccaatac
    15361 cgatataacc gaaaacggta ggtaacgtgt ctttgatcgc agtttttata tctaactttt
    15421 catccaataa caaaactcct ttagcacgac ataaattttc ataattgtcg ttaaacttgt
    15481 gaatattcta aatttttaat gagcatttgt caatggtggg attgaaacgt aaagaactag
    15541 cgggtcgcag atttacacgt attgtttcgg tgggaggagg cgcgaaaaat cttgcatggc
    15601 tacaaatcca agctgatatt ttcgatgcaa cggtctattg tttaagtgtg gaggaaggac
    15661 ctgcattagg cgcaacaatg attgctgcac tagggttagg gtggttcgac acacctgaaa
    15721 gttgttgtca gaattttatc caatatacag atgcagtgga acctctctta caaaatgtag
    15781 aaaagtatca taaaatctat caacaatgcc ggaaaatcta tccagcaacg aaggaaattt
    15841 gtcatgactt ataaaacaaa acgcgtacct aatagtcaaa tgactaatat tgggtacgcg
    15901 ttaagtcttc tttacttcat cctttgcatg attcgtgttt ttaaagcaag gtctgctgta
    15961 tttcttgtgg atttaggatg gttgttatcg tttacttgct atttttcatt gaaatcgtct
    16021 ttctttaccg tttctttagt tttttatcta tttttttctt ttggttagaa aattcttttt
    16081 cccgaaagtc ttctttcagg atttttatag agtagcgttt tgaataagag gcgtcagtat
    16141 gatcgacttg gcagacgaac caagagaaca tttgcctttt ttctgcatga ggtgcttctg
    16201 ctaataattc tttccataaa taataacagt cctctatcag aagaagggtt cgttcatcct
    16261 caggctccca atagaatgtt tcgagagtag caatcacttc attgatcaat tcgaatgcgg
    16321 gaagataggc attttgctga ataagcgatt caactgcttc ttggatgaac tttcgcatct
    16381 ccatgatgaa cttcgctgtt ttcctgtttt tgtttttctt gataatatga tcgtatttat
    16441 ggatgatagt tgttaattta tctgaataat ttattgcatg aaatggcatc ttaactttga
    16501 agcgttcgat aaagtgcgga tgttctataa gaatctctgt taaaagctcc cgtacaagag
    16561 aggaactagc ggaggaaaca agggacgaga gttcggaaag tgagtagttg gattttttta
    16621 ttggacgttc ttcgtaagcg aataagacag cagccatgtg tttgcatttt tttccttcta
    16681 tggcatatag acaatcacaa gtcatgttgg tcttttcatc ccaagtgatc actaccttat
    16741 aatcttccgt gccagaaact gttgcggaca agtacttgct tgtatagttc aaatgacgga
    16801 caaaaccgtg cagatgatag ctgtaaccgc gttccaaaac gttttcagga aaatactcaa
    16861 gccaattatt catctagttc tcctttcatg aaacacttat ttatatttag tataaagaaa
    16921 cgtttaagaa aaaagtagct gattttacat atttgataat cacttttgct cgaattgtca
    16981 aaaattcatt ctccttttct tttcttagag aatgtgagaa aaagtaaaag aatagcgtgt
    17041 tttaaagatg gaactttttt tgttttttat ttatctgaca aaaatgaagt gattttgtta
    17101 gaaataaata attgttccaa ttgataaaag aggtatgatg tatcgcaaag a
//
LOCUS       DANKMN010000065        16767 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000065 DANKMN010000000
VERSION     DANKMN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..301)
                     /locus_tag="PW224_002144"
     CDS             complement(<1..301)
                     /locus_tag="PW224_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2908809.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDK"
     gene            complement(303..500)
                     /locus_tag="PW224_002145"
     CDS             complement(303..500)
                     /locus_tag="PW224_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL2908810.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(894..2621)
                     /locus_tag="PW224_002146"
     CDS             complement(894..2621)
                     /locus_tag="PW224_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL2908811.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2614..3807)
                     /locus_tag="PW224_002147"
     CDS             complement(2614..3807)
                     /locus_tag="PW224_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2908812.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3994..4638)
                     /locus_tag="PW224_002148"
     CDS             complement(3994..4638)
                     /locus_tag="PW224_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2908813.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4732..4866)
                     /locus_tag="PW224_002149"
     CDS             complement(4732..4866)
                     /locus_tag="PW224_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL2908814.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4868..7000)
                     /gene="feoB"
                     /locus_tag="PW224_002150"
     CDS             complement(4868..7000)
                     /gene="feoB"
                     /locus_tag="PW224_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL2908815.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6997..7470)
                     /locus_tag="PW224_002151"
     CDS             complement(6997..7470)
                     /locus_tag="PW224_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL2908816.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7871..8095
                     /gene="nrdH"
                     /locus_tag="PW224_002152"
     CDS             7871..8095
                     /gene="nrdH"
                     /locus_tag="PW224_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL2908817.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8254..10413
                     /gene="nrdE"
                     /locus_tag="PW224_002153"
     CDS             8254..10413
                     /gene="nrdE"
                     /locus_tag="PW224_002153"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL2908818.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10463..11428
                     /gene="nrdF"
                     /locus_tag="PW224_002154"
     CDS             10463..11428
                     /gene="nrdF"
                     /locus_tag="PW224_002154"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL2908819.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11517..12656)
                     /locus_tag="PW224_002155"
     CDS             complement(11517..12656)
                     /locus_tag="PW224_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2908820.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12965..13678)
                     /locus_tag="PW224_002156"
     CDS             complement(12965..13678)
                     /locus_tag="PW224_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL2908821.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13932..14633)
                     /gene="nagB"
                     /locus_tag="PW224_002157"
     CDS             complement(13932..14633)
                     /gene="nagB"
                     /locus_tag="PW224_002157"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL2908822.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14867..16399
                     /locus_tag="PW224_002158"
     CDS             14867..16399
                     /locus_tag="PW224_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2908823.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16590..>16767)
                     /locus_tag="PW224_002159"
     CDS             complement(16590..>16767)
                     /locus_tag="PW224_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2908824.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5205 a         3452 c         2886 g         5224 t
ORIGIN      
        1 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
       61 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      121 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      181 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      241 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      301 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      361 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      421 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      481 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      541 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      601 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      661 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      721 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
      781 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
      841 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
      901 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
      961 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1021 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1081 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1141 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1201 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1261 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1321 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1381 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1441 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1501 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1561 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1621 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1681 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1741 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     1801 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     1861 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     1921 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     1981 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2041 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2101 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2161 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2221 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2281 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2341 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2401 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2461 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2521 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2581 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2641 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2701 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     2761 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     2821 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     2881 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     2941 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3001 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3061 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3121 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3181 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3241 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3301 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3361 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3421 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3481 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3541 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3601 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3661 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3721 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     3781 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     3841 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     3901 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     3961 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4021 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4081 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4141 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4201 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4261 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4321 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4381 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4441 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4501 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4561 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4621 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4681 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4741 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     4801 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     4861 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     4921 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     4981 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5041 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5101 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5161 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5221 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5281 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5341 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5401 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5461 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5521 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5581 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5641 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5701 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     5761 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     5821 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     5881 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     5941 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6001 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6061 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6121 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6181 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6241 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6301 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6361 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6421 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6481 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6541 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6601 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6661 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6721 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     6781 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     6841 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     6901 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     6961 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7021 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7081 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7141 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7201 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7261 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7321 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7381 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7441 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7501 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7561 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7621 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7681 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7741 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     7801 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     7861 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     7921 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     7981 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8041 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8101 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8161 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8221 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8281 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8341 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8401 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8461 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8521 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8581 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8641 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8701 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     8761 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     8821 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     8881 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     8941 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9001 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9061 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9121 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9181 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9241 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9301 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9361 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9421 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9481 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9541 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9601 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9661 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9721 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
     9781 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
     9841 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
     9901 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
     9961 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10021 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10081 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10141 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10201 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10261 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10321 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10381 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10441 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10501 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10561 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10621 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10681 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10741 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    10801 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    10861 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    10921 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    10981 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11041 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11101 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11161 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11221 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11281 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11341 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11401 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11461 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11521 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11581 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11641 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11701 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    11761 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    11821 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    11881 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    11941 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12001 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12061 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12121 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12181 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12241 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12301 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12361 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12421 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12481 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12541 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12601 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12661 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12721 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    12781 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    12841 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    12901 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    12961 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13021 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13081 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13141 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13201 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13261 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13321 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13381 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13441 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13501 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13561 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13621 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13681 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13741 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    13801 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    13861 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    13921 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    13981 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14041 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14101 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14161 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14221 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14281 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14341 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14401 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14461 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14521 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14581 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14641 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14701 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    14761 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    14821 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    14881 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    14941 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15001 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15061 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15121 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15181 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15241 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15301 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15361 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15421 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15481 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15541 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15601 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15661 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15721 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    15781 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    15841 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    15901 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    15961 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16021 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16081 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16141 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16201 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16261 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16321 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16381 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16441 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16501 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16561 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16621 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16681 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16741 ccaataaagt acataaacat aaataca
//
LOCUS       DANKMN010000066        16558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000066 DANKMN010000000
VERSION     DANKMN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..468
                     /gene="scm"
                     /locus_tag="PW224_002160"
     CDS             <1..468
                     /gene="scm"
                     /locus_tag="PW224_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2908825.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(623..1609)
                     /locus_tag="PW224_002161"
     CDS             complement(623..1609)
                     /locus_tag="PW224_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2908826.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1632..2198)
                     /locus_tag="PW224_002162"
     CDS             complement(1632..2198)
                     /locus_tag="PW224_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL2908827.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2212..2628)
                     /locus_tag="PW224_002163"
     CDS             complement(2212..2628)
                     /locus_tag="PW224_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2908828.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3085..5019)
                     /locus_tag="PW224_002164"
     CDS             complement(3085..5019)
                     /locus_tag="PW224_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2908829.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5300..6256
                     /locus_tag="PW224_002165"
     CDS             5300..6256
                     /locus_tag="PW224_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL2908830.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6346..7449
                     /locus_tag="PW224_002166"
     CDS             6346..7449
                     /locus_tag="PW224_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2908831.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7462..8415
                     /gene="menA"
                     /locus_tag="PW224_002167"
     CDS             7462..8415
                     /gene="menA"
                     /locus_tag="PW224_002167"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL2908832.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9350..10696)
                     /gene="gor"
                     /locus_tag="PW224_002168"
     CDS             complement(9350..10696)
                     /gene="gor"
                     /locus_tag="PW224_002168"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL2908833.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10830..12083)
                     /locus_tag="PW224_002169"
     CDS             complement(10830..12083)
                     /locus_tag="PW224_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2908834.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12089..13012)
                     /locus_tag="PW224_002170"
     CDS             complement(12089..13012)
                     /locus_tag="PW224_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2908835.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13233..15725)
                     /locus_tag="PW224_002171"
     CDS             complement(13233..15725)
                     /locus_tag="PW224_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2908836.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15732..16199)
                     /locus_tag="PW224_002172"
     CDS             complement(15732..16199)
                     /locus_tag="PW224_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL2908837.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16335..16408)
                     /locus_tag="PW224_002173"
     tRNA            complement(16335..16408)
                     /locus_tag="PW224_002173"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16372..16374),aa:Arg,seq:acg)
     gene            complement(16466..16539)
                     /locus_tag="PW224_002174"
     tRNA            complement(16466..16539)
                     /locus_tag="PW224_002174"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16503..16505),aa:Pro,seq:tgg)
BASE COUNT         4887 a         3460 c         2944 g         5267 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagctc agattcatct
      121 accactacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga tacatcctct
      181 accacaagtg atagctcaga ttcatcaact tcaagcgata gtacagacac gtcgtctacc
      241 acaagcgata cacgtcatgc aagtgtctcc ttctcaaaaa caagttcgga taacaggacg
      301 aactttaact taactaataa aacaagcaat gacaatggaa acaaatcaaa aagagcactt
      361 cctaaaactg gaagtcaatc gaataattgg ataacactcg caggcgttat attacttgta
      421 atcggtttaa gaatcacttt tatgagttat aagaaatcga aaaggtaaaa aaatattcaa
      481 gataaaaaag agcaacgtcg ctaacggacg ttgctcttta tttttgcttt ttatcataaa
      541 tacaagaact acaattcaat ttgtcaaaaa acacgtagat ttccttctga gaaatttatt
      601 tacgtatcga acctttccct attcagtctt tgcgatccaa aatcagttgg gtaagggaaa
      661 agagattctc ttttgttcga agagaggtgg caggtaattt ctttatttcc ttcaaagcct
      721 tttctgtgta agaggccgct aacttcctag cttcttccac gcctcctgta tgttcaacga
      781 ttttgcgtac ggcgtcgaca tcttcttctg tcattgccgc tttcttctct aaataaggta
      841 gtagctttgg tttgtttttt tgcaaagagt agatcaacgg taaagaatag accccttgac
      901 gcatgtcttc tagtacaggt ttgccgattt cctggctctc ttttgtataa tctaaaatat
      961 catcgataat ctgaaaggcc atcccgatag aaagtccgat atctcctgct ttttttgcga
     1021 agcgctcgct tgttccactt tcaaaagcgc caacagaaca acttaattgg aacagttcag
     1081 ctgtcttacc tgaaatattt tccaaatatt cttttacaga aagttcataa ttatagcgat
     1141 catccatctg ccctaattcg ccattcaata ctttttccat actgcgagaa ttcaattgga
     1201 gacttttcaa ggaagtggag taatcagaca atagtttaaa acagcagaca aataaatagt
     1261 ctcctgcata gacagaaacg acattaccaa actgactacg gatattaggc tgaccacgtc
     1321 gcagatccgc ttcatcaacg atatcatcat ggatcagtgt ggccgtatgg agcatttcga
     1381 tagaagctgc caaagcgatg gctttgtctc gatcttgctc tggtccaaat tgtgagaaca
     1441 atagctgata ggcaggacgc aacagctttc cgccggcatg gatcgtttct ttgattgctt
     1501 gttcgacagg tttgtttttc aggcggacag tttcttccat ccgttttagt gtttctgtca
     1561 attctttact taaagcagga tagttttccc acattggatg tagcttcata aaaaaactcc
     1621 ttgtgtgacg attatttttt ggtaaacatt gggctccatt tttgtttggt tccctgctca
     1681 taatcttttt ggaatcgttg aagtgtctgc acatgtcgta aaagataatt cgctgcgatc
     1741 ccgattgcaa tccctgataa aatccctaaa actgaaagga ctggcaagta caacatgact
     1801 gtccatgatt gtgcaatgaa agaagccatc aaaagctgtc cgacattatg catgaaaccg
     1861 ccggccgcac taattccgat gatactgaca cgttttggtc ctaattgctt gaccaataac
     1921 attccaaaat aactaagcaa agcaccactc atactataaa ggaatgtgga aatcgttccg
     1981 cctagcaaag tagtcaggaa tagtcgtagc caaagtagta agaagctgtc ttgttttttc
     2041 atggtaaaaa tggcaacgat cgtgattaga ttggctaagc ctagttttgc accaggcgca
     2101 aaagaaaaag gataagggat catattttcc aataaaccaa tgatcactcc ttgtgcaaca
     2161 agtagcgaaa tataaatcat tttttgtaat ttactcatgg tgtgtaaagg tctaataaat
     2221 cagactgtct tcgtcacctc catctgttga acggacttcg atcactaatt tatgtggcaa
     2281 acagacgatg gtttcgccat ttttcgttgc ccagcctcga cgtacgcaga tctggtcatc
     2341 gcaatctgct tcttttatcc gtactcgatc gccatctatc tcgatcaagt tataatctcc
     2401 gtgttcatct ttgtaagtat atgtgttgct ctcttctcca gccatcaaag ggaatgtttt
     2461 gatttctgtg ccatccacac gcagtaccgc ttctttttct gctgttgcgg tgttttgctg
     2521 aagaccaaaa atcatgactg gtaaaaaaga aagaacagtc aaaagcaaaa taataaaaac
     2581 atcccaaggt ttgagcagac ttttttcagc aaattcttta aggctcattt ttgttctcct
     2641 ttttccagat taactcaccc aattttagca taaaaacaag aggttgtgac acctgtgtct
     2701 tgctttgagt aataagaacg aagaatagaa aacagctttc catgttttct aattctttgg
     2761 gcttattacc gagaagcagc aggtggaaca ccgtttataa caagaggttg tgacagaagt
     2821 gggcaacttc aagaaataag aaggaattta cgaaaattac ttttcaagtt tttgtgaaat
     2881 ttcggcttat ttttcgaaga agctacttct ggaacaccgt ttataacaag aggttgtaac
     2941 agaggttgtg acacctgtgt cttgctcatg gaggcttatc tcgggcatct tattccttta
     3001 attttacata aaaaggaggc tgtgacggtt tgtctcagcc cctttctatt gaaatcttgc
     3061 attcaaataa cgttctttat ctatttattt cttgccgtac tgggaacttg gtctaccgta
     3121 ccaccaatta cctagtgtac gttgtagcca tggaattacc cagcgatcca acccaatcgc
     3181 acgtccggaa ccgttcatca atgcgaaagc aacaaaaatg aaccaaatgt ttacccagta
     3241 aaacatgcct gataagcaga acgtgatcac caatacaatc gttgtcgcac tgcatagcca
     3301 tgtgaataat ccagcaatca atgctaatgc caaggcaatt tcaaagaata ccatgaattt
     3361 ttggaagaac attgctacat cattgtttgg catgatcact tgcatgatct ttgtgaacca
     3421 gtctggtgac ttctcaatca cagccattgg ttcttcaccg tatgcataac tcaaaccaaa
     3481 atgtgcagct tgagtcgctg cttctccggc acttgcccct gctccgctgg cagctgtggt
     3541 tgcatcacca gcagcagaag caccactggc aacatctgtt acattgtatt cccatgtcca
     3601 agggaacaca ctgtttccag tgaaccatga tccttcacca aaccaagtac ttggtttgaa
     3661 cacttcacca ttcccaatga tttttttgga agcttcaacg aaccaaacta aaccatagaa
     3721 aatacgaagt ggcacactcc ataagacatt gccgtaacga gaagaatgtc ctcgagtcac
     3781 gttgcgatca tctttgatat ggaaaaactc atgcattaaa tattggaaca tataataacc
     3841 agaacgaata tcaaagaagt atttcaaatt cacgatatgt ttcattacga tagctaaaaa
     3901 tccgcttaaa tggatcttat caaataggtt agctaccccc catttggcac caatagaaac
     3961 cataaatcct tgatagtttc ctttaaattt atgcttttct ccgcctttga tcgaagccac
     4021 gatattcgct gcggctgtgt gtcctgtttg ttcagcagct tgaacgatct gcggtgtcgg
     4081 tgttcctggg aattcttcat aaaagactaa atcaccaatg atatagatgt ttttatcttc
     4141 gtagcctttt gcctgcatat attcattggc gatcaaccgc tgtccgcgtg ctgattctaa
     4201 tccaaagtca gcagcgtcag aagttccttt gacaccagct gtccaaatca gtgtgtgagt
     4261 cggaatgttg cgtccgtctt tcaatttgat atgatcagca gccacttcta cgattggtgc
     4321 attcaataat aattccacgt tgtgcttacg caaatagcgt tcagcttttg cagcatcatt
     4381 tcgatccaac atattcaaga tcgtcggcat tgcctctacg accatcagtg tgatttcgtt
     4441 tggatctaat ttataatctt tggctaggcg atttttccag tcgatcagtt ccccaatcat
     4501 ttcaataccg gtaaatccag aaccacagac aacgaaagtc agcatggctt tacgcagttc
     4561 atcatctggt tccaatgcag cttttgctac tgtcttctca atatgttccc gaatccttac
     4621 agaatcttca aatgaccaaa gggtgaatcc gtgtgctttg acaccaggtg taccaaagtc
     4681 attcggctct ccgcccattc caaggatcag ctgatcaaaa ggataggaac ctaatttggt
     4741 cttgacgatt tttcgttctt tatcgatacc gattacagtg tctgtgacaa gttgtacatt
     4801 cttttgacga gcaaacaaat gctgcagatc gtattggatt gcttctggtt ctacacgtcc
     4861 gccagctact tcatgcaact cggtcatcat agtatggtac gaatgacgat cgatcagagt
     4921 aattgctaca tcttcttttt tcagtttctt tgccaagtat tttgtagctg aaacaccggc
     4981 atatcctgct cctacaacga caatattttg ctttgccatt gatcatccgc tccttataga
     5041 ctatctatat aatgaatata tgataattct tttagtttga tacttattac acaatctcca
     5101 tatattatat agatgttctg ttcttttgtc taactccttt tggatttttt tcaaatttta
     5161 aaataaagcg ttttttcacg aaatttgctg aaagaaaacg tttgaaatat tacatgggtt
     5221 atttatcaaa aatagttgac ttataggcta aaaagattag aatgtataat gaatcacaaa
     5281 gaaaaattgt ggactagaaa tggaatggtg gggtattaaa atgaagaaaa aacgttttgt
     5341 aactggattt gccgtattag cattttcagc tctagtgtta ggcgcttgcg gcgcagatga
     5401 caacggaagc tcaaactctt cttctgaatc atctacagca caatcttcga ctgcaaaaag
     5461 cacaacagaa tcatctgcaa aagttgtagc tggcggagat ttgcaagacg gcacatataa
     5521 attagaagaa aaaaattatt caaatggtta ccgtgcagtc tttgaaatgg ttgtaaaaga
     5581 cggcaaaatc actgaatcaa aatatgataa cgtcaatgaa aacggcgaat caaaaacaaa
     5641 agatgcagaa tacaacaaaa acatggaagc aaaatctggc acaaacccag aaaaatacat
     5701 tcctgagttg aatgaccaat tcttaaaagc acaaagcgca agcggtgtgg aagtcgtaac
     5761 tggagctact cactcatctg aaagcttcca aaactacgca caacaattga tccaagctgc
     5821 tcaagctggt aacacagata caatcgaaat cgacaatggt gctgatttga aagacggcac
     5881 ttacaagctt gaagaaaaga actactctaa cggctaccgt gtgcaattcg aaatgacagt
     5941 tgctggcggc aaagtaactg aatcaaactt tgattatatc gacaaagacg gtaaatcaaa
     6001 acaagatgat acagaatata atgaaaatat gaaagcaaaa agcggtacag aaccaaaaac
     6061 ttacatccct actctgaacg acgaattcgt caaagcaatg ggcgaagaag atggctcacc
     6121 tgctaatgta gaagttgtta caggtgctac acacagctca cattcattca tcatgtacgc
     6181 acaacaatta gtgaatgcag ctgaaaaagg cgacactcaa acaatcgaag tagataatat
     6241 cgtaacagaa aaataatcac ataatcatac taataaaagt tggagggtgg gacgatagcc
     6301 agattaggca gtcgtctcac ctttcaaaat gaagagagga aaaagatgcg gaaaaaacta
     6361 ctattttcct taatcacact gggcatctct tttatggtgc taactggctg cagcaactca
     6421 gcaaataaaa caaccactga aagttcagca gctaccaaga tattgaaaga tccatattct
     6481 gatgaacagt tcttgttagg gacttacgtt cgtatccgtg tgtatgatga aggcaaagaa
     6541 tcagcattga aacctgcctt cgatcgagta aaagaacttg gtgataaaat cacgatcaat
     6601 caaaaaggtt ctgagatcga tgaagtgaat gagcaagctg ggatcaaacc agtaaaagtt
     6661 tctgatgacg tctacacgtt agtcaagcgg gcctacgaat atagccaaga ttctcaaggc
     6721 ggattcgata tggcgattgg tgcaatcaca caattatggc gaatcggttt tgatgacgcc
     6781 agaaaacctt cccaagaaga aatcgatcag gcattgaaat tagtggatta tcataagatc
     6841 gaactaaatg acaaagaaaa aactgtttat ctaaaagaaa aaggaatgat catcgattta
     6901 ggtgcaatcg ccaaaggtta cattacggac gaagtagtga aagtcttgaa aaaacaagga
     6961 gtgaccactg ccatcgttga cttgggcggt aatgtctatg tcttaggcca cagcccaaga
     7021 ggcgaaaacc aagactggac agtcggcatc caagatccga acatggctcg cggatctgtt
     7081 ttaggttcta ttaaagaacg gaacaaaaca ttagttacct ctggaattta cgagcgttat
     7141 ttagaagtag atggcaaaaa ataccatcat ttgtttgatc caaaaacggg ttatccgttc
     7201 gacaatgata tcgcaagtgt cacgatcata acagataaat caattgatgg tgacgggttg
     7261 tctacagcag tcttttcaat gggtgtgaaa aaaggactgg aatacgtcga aagcgaattg
     7321 aataacggga cggaagcgat cttcgtgaca aaagacgata aagtatatgt gaccgatcca
     7381 atcaaagaca ccttcaaatt atcggaggac tcccattaca cgatgggtga ccgttctgaa
     7441 ctaaaataag gagtacgaac catgacacta aaaatctttt tagaagtagt tgaaatacga
     7501 acaaaggtgg ctagtgtatt tccttttgcg gtaggggtat tattctctat cgcttttttt
     7561 catgagatac attggctaaa cactttttta ttttttattg gaatgctagt ttttgattta
     7621 gcgactaccg ctatcaataa ttacatggat tatgaaaagg ctcattctga agtatataag
     7681 tatgaagaaa atgtcattgg ccgcagaaat ctatctcctg tattgattcg aaacatgatt
     7741 ttagctatga ttgcatttgt ttttgttatc ggcgttattc taacgatcaa tacgggttgg
     7801 cttttacttt tgatgggatt tatctgttgt tttattggag tattctatac attcggaccg
     7861 attcctttat ctcgaatgcc tttaggggaa attttcagtg gggtgactat gggccttgga
     7921 atttttgcca tggtcgttta tgtcaataca ttcggttcag atgtctatga tctgacagtc
     7981 aacttttcat ctggacaatt tgctttggta ggaaatcttt gggctatagc agcaatcgta
     8041 ttagcttcct tacccttggt gtttacgatt gccaatatca tgttggctaa taatttgcgg
     8101 gatcttgacc gtgatattga aaatcatcgc tatacactag ttttttatat cggacgtcct
     8161 gcgggaacag tcttattcca gctattgatg tatgcttgct atttcgtgat tttaatgggg
     8221 ttgttgagcg gtatctatga atggccgatc ttggtcacct tcttgacctt gccaattgtg
     8281 aagaagcatt tggatcaatt caagcaaacg ttaccccagc ctatcagttt cagctattcc
     8341 atcaaaaata tggtcttatt caatgccagt tatgtattgg ggctattact aacaatcatt
     8401 atgcaagcca tttaacagca tgaagaggtt gtgaaaaaac tgccctgcat caagtaataa
     8461 gaacagccaa ataaaaatag ctccttatat ttttcgtttg gttgggctta ttaccgcaga
     8521 tgcaagcttt tgaacaccgt ttatcaggag gttgtgaaaa agtcgcttag tttcgagtat
     8581 taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga
     8641 gaaactggct tttgaacacc gtttatcaga ggttgtgaaa aagtcgctta gtttcgagta
     8701 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg
     8761 agaaactggc ttttgaacac cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt
     8821 attagagaaa caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg
     8881 aaggacagct gattgaacac tatttgttcg gaaaaagtga ctaactatga cacaattttt
     8941 ttcacagtcc cttttttcac ttgatttgat tcgtaccagc gaacttgacg agaactttct
     9001 tggtttggta actatatggt attcttttaa catcctcgtt tgcttcttct aatttattta
     9061 tacaaagtca cactttttta aacatctgtt cttcgataca tttctatttc ttttaaacat
     9121 cgataccaat attttgaact taagagtgca taccaaatgg attcatttac tcttcgtcag
     9181 ttacgaagat aatcaaaatc tccaacttcc ctacttattt cgttttgaga attatcgaag
     9241 caatcttaca taaataacta aaaaacaaaa aaccagaaaa tcagtatact gattttctgg
     9301 ttttacatgg tttgttcaat aaaaagctgt tggaactgtt aatattaaat tatcgcatcg
     9361 ttacgaattc ttccgctcct gttggatgga tggctactgt attgtcaaag tcttctttag
     9421 ttgcacccat cttgatagcc acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa
     9481 tagcatgtaa gccgacgatt ctttcttctt ttccgacaca gatcaatttc atttcgcatt
     9541 tttggcgata atcatttaat gcaaagtaca ttggggtaaa gcgtgaacga taaactttca
     9601 attcattttc gccatatttt tccaatgctt cttcttctgt caaaccaatt gttgcaattg
     9661 gtggatgcgt aaagacgact gtcggaacaa gtttatagtc aagataagaa tctttctttc
     9721 cattaaataa acgttctgat aaacgtctac cagctgcgat tgcaacagga gtcagatcta
     9781 atttaccgat cacatcacca acagcaaaaa tatgttcatc cgttgtattt tggtatttat
     9841 ctactttgat aaaccctttt ttgtccaatt ccacatttgt attttccaga cctaattttt
     9901 cggtattcgg tacacgtcca ccggcaaaaa gtacacaatc accagttaag gtttcaccat
     9961 tttcaaatag caccgtatat tcgtcattgt ttttagtaat ttctttagca atatgatgtg
    10021 cgtaagtttt tattccagct tcttggtaca tttggatcaa attgtccgaa agcattttat
    10081 caaacgttct aagtggtcgt tcttttcgat atgcccacat gacttcacta cctaaaccac
    10141 gaaatacacc tgaaagttca gccgcaatgt atccagcacc taaaacgatt gctctcttag
    10201 gtaactctgt taattcaaaa aatccatttg aatcaattgc gtattctcca ccaggaatgg
    10261 tcattttact tgcacggcct ccagtagcga tcaagatttt aggagcacga taggtttcac
    10321 cgtttacttc gatcgtttga ctatcgataa aagtagcgta accttttata gcctcgacat
    10381 gattgctatc taatcctctt tgataagcac cgtgtagaaa atcaatatat ttttctcgtc
    10441 tttctactag accggcgaaa tcaacatttt ttaaatctgc ttggataccg taacttgagg
    10501 catctcgttc aattgtttcc aaaatcgtac ttgcttgcca cataactttt ttaggtacac
    10561 aaccgacgtt gacacatgtt ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc
    10621 catgcatccc tgctctgttt gctgaagcaa taccgccaga gccgccacca attacgatgt
    10681 aatcatatat ttgcattttg ttcctcctta ttgaagagat caatcaaaac ctttatttaa
    10741 cgatcctttt tctacagaaa acaagtgtaa cacttttctt gaattttgta tgcgaagatg
    10801 cttaaaacag ggatatatca gctttttcct tatttacgtt ttcggatcat atctccaggt
    10861 ttcaccggct gtgcgactgg catagttaaa atcatcattg gatttggtgc tcggtcaatg
    10921 gattcgcctt cttcattatg cataacttca acggtttgac tgaaatgact gaatcctgga
    10981 ccataaaatt cgatttcatc tcctacagaa aacaagtttc tctgacgtat cgtagcaatc
    11041 tgtgttgcct catcataaga taatacttca ccgacaaatt tatatactgg gattttacga
    11101 cgttcaccga ataattgttc gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat
    11161 tctcgttgtg ctactttcca taactcgtcg atccattctt gcttgcagac atagttttca
    11221 ggatcttcca tataactgtc tacagccgct ttatagacat ttgacaccgt agatacataa
    11281 tggatcgatt tcattcttcc ttcgatcttg aagctgtcta cgccgttttc aatcaattct
    11341 ggaatatgtt cgatcattga catatccact gcactcatcg agaattcttc ttccacttcg
    11401 cctgtatcag ttaaactgtt tcgttcttga ccaaaaggca tatcgaacaa tccatacttc
    11461 cagcgacaag attgagaaca tccgccacgg tttgcatccc gcatcgacat atggttggac
    11521 aatgtgcatc gaccagaata agagatacac atggcaccat ggataaatgc ttcgatttcg
    11581 gtatccgtat ttttacgaat ctctgccact tcctccattg aaacttcacg agctaaaacg
    11641 acacgttcca acccttcttc tttccagaat tccaatgtct caaaattagt tgctgaagct
    11701 tgagtggata aatggatcgg cagacctggg gcttctgtcg cacaaatttc gatcaaagct
    11761 ggatcagaaa caatcactgc agaaataccg acatcacgca aactgcggaa aaattcccct
    11821 gctccatctt catttccttc atgggtgacc atattagcag caacgtatac cttcgcatca
    11881 tgttcttttg caaaagcaac tccttcagcc atttcttcat aagtaaaatt acctgcacgg
    11941 cttcttaaac catatgcatt cccgccaata tatactgcat ctgctccata atggatcgcg
    12001 gtttttaact tttccagtgt tcctgctggc gctaggattt ctggtcgttt caattttctt
    12061 tttactgtca tttctttcat catactcctt atttgatagc agaaggatca atataaaaga
    12121 atcctgtatc taattcacgg ttttcaggat gcaatttact gatttcgcta gtcatttttt
    12181 ccgctttttc ttgatcccag ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc
    12241 gagtgatgtt tacgaatttt tcgcctggag taaataatcc gtccaatttc catgtacagt
    12301 aatgatgctg ataaagttca ttcaattcat tcgagagatt cagatcattg ctggcaaaaa
    12361 tatgtgtgcc atgactatct tcataaatag aataatgtgt ctcttctttt ttaggttccg
    12421 agataaaaag acctctttca cgatcttttt gttcgtcttg tttcgtatag ttatagtaat
    12481 tctgtagcag tggacgtttg gattgatgga tgcatgtagc accatacaca agggtttcta
    12541 caggaatatc gagtttttct tccatcgcct tcatctcttc aaaaggaact tcacgagcaa
    12601 gtactgctcc aactgcgcct tttttcgccc agaaattgat ttgacgtgcg ctcgttacta
    12661 atgtttcagc atcataaata tacggtatac gcaattcagg attttttttc atcgtataaa
    12721 taatacctgt atcaccaact gtgatcacat ctacgtttat ttcttttaaa aaagccaaat
    12781 attcaggtac atttttcatt ttttcaggat gaatgatccc atttacagca atattcgctt
    12841 ttttaccata cttgtgtgct aattctacca attgtcgctg ttcttctcgt gtaaatgaag
    12901 caggcagacg caatccaaat tgctcttcac cgaaatatag tgtatctgca cctgcagcaa
    12961 gcaactgttc tgcttgttca atagtttcta ctgtggttat gagttcgatc ataattttct
    13021 cctcctacaa cttcataata gtagcatagc tctaaaaact ttccaatact tcttctattt
    13081 aaagtcggat ttttgaaaat tatttcacaa aaaatcaaaa taaagttcga tatattctgt
    13141 ccaaatataa aaagagattg tgacaaaaat cttgtcacaa cctcttaatc aagtcttact
    13201 attataaaaa ttcagctaca caggccaagt aactataggg aacctactgt ttccttttta
    13261 ggtccgcgaa cattcaatgt gatcttacct tttgaagcac ctactgttac gtggtcacct
    13321 aaacgaattt ctccgccaag caaagcctcg ctcaatcgat cttcaatttc tttttgaagt
    13381 gcgcgacgga ttggacgtgc accatattca ggatcaaatc cagctttccc gatcacatcg
    13441 attgcagatg gtgtgatttt cacttgaata tcttgttctt tcaatcgttt gataatcgct
    13501 ttgctcatga tttggacgat tgtatggatt tcatctttac tcaatgaatg gaagacgaca
    13561 gtctcatcga ttcgattcaa gaattcaggt cggaaagctt tctttaactc ttctaagata
    13621 cgtttttgca tggctttgtg atctttagta acatcttgga cattgaatcc gacatttttt
    13681 tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa tcatgattgt atttctaaag
    13741 tccacttttc tacctttaga atctgttaaa tgaccatcat ccaatacttg cagaagtaaa
    13801 ttgaaaacgt ctggatgtgc tttctccact tcatctaaga gaatcactga atatggtttt
    13861 tgacgaattt tttctgtcaa ctgtccacct tcatcatacc ctacgtatcc tggaggagac
    13921 ccgattaatc ggcttgtact gtatttttcc ataaattctg acatatcaac acggatcagg
    13981 gcatcttcac ttccaaacat gacttctgaa agtgctttgg ccaattctgt ttttccgaca
    14041 ccagttggtc caaggaacat gaatgaacca attggacgat caggatcttt caatccgctt
    14101 cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct catcttgacc aacgacacgt
    14161 tcatgcagaa gtccttccag ttctaacaag cgttcgcttt cttttttctc caactgctgt
    14221 agagggacac cagtccactc cgatacaact gtagcaacat cttcttctgt tacacgatca
    14281 gaataacctt gctcctgttt tgcttcaatt gcagaaactt tttgcagctt tttacgtagt
    14341 gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt cttgtttttg gatcgctgct
    14401 tccttttctt gtactagttc atcgatttct agtttgatca cagttgattt tgttaagtga
    14461 tcggtttgat ccaaacggac tttcgctgct gattcatcga tcaaatcaat tgctttatcg
    14521 ggtagttgac gagaagtgat ataacgaaca gaaagattga ctgctgctcg tacagcttca
    14581 tctgtgatct ccactccatg gtgctcttca tatcttgtac gtaatccttg aagaatcact
    14641 tctgcttctt caggagttgg ctcatctact tgaatgcgag caaaacgtcg ctctaatgca
    14701 gaatcttttt cgatatattt ttgatattca tctagggtcg ttgctccgat cgtttgtaat
    14761 tctcctcgag ctaacgccgg tttcaagata ttcgaagcat caattgctcc ttctgctcca
    14821 cctgcaccaa ttaaggtatg caattcatca atgaataaga ttacttgacc atcattatag
    14881 atttcgtcga tgaccttttt cattcgatct tcaaattcac cgcgatattt cgtaccagca
    14941 accaaagatc ccatatcaag catcatcagg cgcttttctt gcatgtcttc tggcacttca
    15001 cctaaaatga ttttttgagc taacccttca gcaatggctg ttttgcctac accaggttca
    15061 cccaccagta cagggttatt ctttgtccgt cggctaagaa tctggataag ccgtttcact
    15121 tctctcgaac gaccaacaac tgggtccagc ttgttttctt ttgctaattt cgtcaaatca
    15181 cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt gccgtttatc ttgtacattt
    15241 acatttctac gacgattcat tccttttcca gcagaatcac cagctagccc cattttttgt
    15301 ttcagaactt gacgggcttt tgaaaggctg atacctaagt tgatcatgat tcgagtagcc
    15361 atgatacttt catcttttaa taagctcatc aaaatatgtt ctgttccaac tttttgagaa
    15421 ccagttttct tcacttcgct gctagctaaa gcaaaaactt gtttcattct tggtgaatat
    15481 ggaagaaaga ctcctttagg gtaagaacgg ataccaccat aaccaatcag atgttcaatt
    15541 tcttcataaa tttccgtttc attcaaatcc atttcacgta atacttttcc agcaattcca
    15601 ttttgctcga ccactaaggc tagtaaaaca tgttctgaac taactgattg atgtttcaac
    15661 cgttttgctt cttcttgtgc aatttctaat actgcttggg ctctttcagt aaaaagttct
    15721 tccatctaat ctcacttccc atcttcataa cttaaacgtt ccagaaatgc tcggagaatc
    15781 cgagcacgtg tatgatcttc atattcattg aaattcaatg tgttttttgc aattgcactc
    15841 aacatcaagt ttccttcttt ttttgtaatt atttggtctt cataaagttt ttgaataata
    15901 gcaaaagcat ttttctcact gattgtatca tcaaataact gatttatctg ctcaagtaat
    15961 tgttttttat ctgaaatcct tacttttgca attcgtatat aaccaccacc accacgtttg
    16021 ctttctacta gataaccacg ttggatggta aaacgggtat taatcacata gttgatttgt
    16081 gaaggaacac agttaaagag gtctgccatt tcaatccttc gaatctcgat catttcacta
    16141 ttttccagaa ttttctttat atatgattca attaaatccg aggtattctg atgtgccatc
    16201 ttattcattc cctttgacta atattgacct taattataac gaatttgata atagaaagaa
    16261 agaagaacgc tttttgttaa ataaatcctt tagaatcaac aaaaaaaaat acggaatata
    16321 ctattccgta tcgacgcgcc caagaggagt cgaaccccta accttttgat ccgtagtcaa
    16381 acactctatc cagttgagct atgggcgcat attcaattta aaaaaattat aaatcatttg
    16441 aaaacaaaaa gcaagataaa aaaaatcggg aagacaggat tcgaacctgc gaccccttgg
    16501 tcccaaacca agtgctctac caagctgagc tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DANKMN010000067        15945 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000067 DANKMN010000000
VERSION     DANKMN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            226..894
                     /locus_tag="PW224_002175"
     CDS             226..894
                     /locus_tag="PW224_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL2908838.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="PW224_002176"
     CDS             complement(1094..1324)
                     /locus_tag="PW224_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908839.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="PW224_002177"
     CDS             complement(1451..2833)
                     /locus_tag="PW224_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2908840.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="PW224_002178"
     CDS             complement(2989..4176)
                     /locus_tag="PW224_002178"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL2908841.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="PW224_002179"
     CDS             complement(4183..5214)
                     /locus_tag="PW224_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2908842.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="PW224_002180"
     CDS             complement(5260..6684)
                     /locus_tag="PW224_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2908843.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="PW224_002181"
     CDS             6859..7764
                     /locus_tag="PW224_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2908844.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="PW224_002182"
     CDS             7855..8532
                     /locus_tag="PW224_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2908845.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="PW224_002183"
     CDS             8529..8699
                     /locus_tag="PW224_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908846.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="PW224_002184"
     CDS             8813..9709
                     /locus_tag="PW224_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2908847.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="PW224_002185"
     CDS             complement(9764..11134)
                     /locus_tag="PW224_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2908848.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="PW224_002186"
     CDS             11372..12118
                     /locus_tag="PW224_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2908849.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="PW224_002187"
     CDS             12115..12954
                     /locus_tag="PW224_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2908850.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="PW224_002188"
     CDS             12955..13905
                     /locus_tag="PW224_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2908851.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="PW224_002189"
     CDS             14046..14798
                     /locus_tag="PW224_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908852.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..>15945
                     /gene="queG"
                     /locus_tag="PW224_002190"
     CDS             14817..>15945
                     /gene="queG"
                     /locus_tag="PW224_002190"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL2908853.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQK"
BASE COUNT         5169 a         2993 c         3215 g         4568 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaat
//
LOCUS       DANKMN010000068        15229 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000068 DANKMN010000000
VERSION     DANKMN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            278..787
                     /locus_tag="PW224_002191"
     CDS             278..787
                     /locus_tag="PW224_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2908854.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="PW224_002192"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="PW224_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2908855.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="PW224_002193"
     CDS             2322..3302
                     /locus_tag="PW224_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2908856.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="PW224_002194"
     CDS             3424..4842
                     /locus_tag="PW224_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDL2908857.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="PW224_002195"
     CDS             4913..6382
                     /locus_tag="PW224_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2908858.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="PW224_002196"
     CDS             6392..6859
                     /locus_tag="PW224_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2908859.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="PW224_002197"
     CDS             6895..7767
                     /locus_tag="PW224_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2908860.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="PW224_002198"
     CDS             7848..10382
                     /locus_tag="PW224_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2908861.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="PW224_002199"
     CDS             10538..11311
                     /locus_tag="PW224_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2908862.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="PW224_002200"
     CDS             11304..12095
                     /locus_tag="PW224_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2908863.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="PW224_002201"
     CDS             12105..12596
                     /locus_tag="PW224_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2908864.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="PW224_002202"
     CDS             12614..13024
                     /locus_tag="PW224_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2908865.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="PW224_002203"
     CDS             13028..14428
                     /locus_tag="PW224_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HDL2908866.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="PW224_002204"
     CDS             14451..14825
                     /locus_tag="PW224_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2908867.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2134 c         3045 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactccta
//
LOCUS       DANKMN010000069        15139 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000069 DANKMN010000000
VERSION     DANKMN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            4..2376
                     /locus_tag="PW224_002205"
     CDS             4..2376
                     /locus_tag="PW224_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908868.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="PW224_002206"
     CDS             2437..3897
                     /locus_tag="PW224_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL2908869.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="PW224_002207"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="PW224_002207"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2908870.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="PW224_002208"
     CDS             6128..6277
                     /locus_tag="PW224_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908871.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="PW224_002209"
     CDS             6352..6945
                     /locus_tag="PW224_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL2908872.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="PW224_002210"
     CDS             6958..7857
                     /locus_tag="PW224_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL2908873.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="PW224_002211"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="PW224_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2908874.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="PW224_002212"
     CDS             8723..8983
                     /locus_tag="PW224_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908875.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="PW224_002213"
     CDS             9428..9634
                     /locus_tag="PW224_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL2908876.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9682..9885
                     /locus_tag="PW224_002214"
     CDS             9682..9885
                     /locus_tag="PW224_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908877.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="PW224_002215"
     CDS             9901..10338
                     /locus_tag="PW224_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908878.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="PW224_002216"
     CDS             10331..11038
                     /locus_tag="PW224_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908879.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11418..11597
                     /locus_tag="PW224_002217"
     CDS             11418..11597
                     /locus_tag="PW224_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908880.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="PW224_002218"
     CDS             11922..12185
                     /locus_tag="PW224_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDL2908881.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="PW224_002219"
     CDS             12179..12529
                     /locus_tag="PW224_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2908882.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="PW224_002220"
     CDS             complement(12553..13167)
                     /locus_tag="PW224_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2908883.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="PW224_002221"
     CDS             13481..13903
                     /locus_tag="PW224_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908884.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="PW224_002222"
     CDS             13913..14116
                     /locus_tag="PW224_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908885.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14327..14911
                     /locus_tag="PW224_002223"
     CDS             14327..14911
                     /locus_tag="PW224_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2908886.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5571 a         2451 c         3061 g         4056 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
    14341 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
    14401 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
    14461 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
    14521 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
    14581 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
    14641 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
    14701 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
    14761 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
    14821 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
    14881 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
    14941 ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact
    15001 tattctcaaa tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa
    15061 aaagtccgta ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt
    15121 ttcaaattaa ttagtaaag
//
LOCUS       DANKMN010000070        15135 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000070 DANKMN010000000
VERSION     DANKMN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..588)
                     /locus_tag="PW224_002224"
     CDS             complement(<1..588)
                     /locus_tag="PW224_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2908887.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELY"
     gene            complement(741..1067)
                     /locus_tag="PW224_002225"
     CDS             complement(741..1067)
                     /locus_tag="PW224_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908888.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(1079..1279)
                     /locus_tag="PW224_002226"
     CDS             complement(1079..1279)
                     /locus_tag="PW224_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908889.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            1402..2004
                     /locus_tag="PW224_002227"
     CDS             1402..2004
                     /locus_tag="PW224_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2908890.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            2059..3225
                     /locus_tag="PW224_002228"
     CDS             2059..3225
                     /locus_tag="PW224_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2908891.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            3642..6869
                     /gene="fss3"
                     /locus_tag="PW224_002229"
     CDS             3642..6869
                     /gene="fss3"
                     /locus_tag="PW224_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2908892.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            6981..7295
                     /locus_tag="PW224_002230"
     CDS             6981..7295
                     /locus_tag="PW224_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2908893.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            7308..7682
                     /locus_tag="PW224_002231"
     CDS             7308..7682
                     /locus_tag="PW224_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2908894.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            7683..8036
                     /locus_tag="PW224_002232"
     CDS             7683..8036
                     /locus_tag="PW224_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL2908895.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            8105..9121
                     /locus_tag="PW224_002233"
     CDS             8105..9121
                     /locus_tag="PW224_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2908896.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            9715..11589
                     /gene="ltrA"
                     /locus_tag="PW224_002234"
     CDS             9715..11589
                     /gene="ltrA"
                     /locus_tag="PW224_002234"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2908897.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            11715..12071
                     /locus_tag="PW224_002235"
     CDS             11715..12071
                     /locus_tag="PW224_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2908898.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            12116..13258
                     /gene="dcm"
                     /locus_tag="PW224_002236"
     CDS             12116..13258
                     /gene="dcm"
                     /locus_tag="PW224_002236"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDL2908899.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            13248..13537
                     /locus_tag="PW224_002237"
                     /pseudo
     CDS             13248..13537
                     /locus_tag="PW224_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13793..14977
                     /locus_tag="PW224_002238"
     CDS             13793..14977
                     /locus_tag="PW224_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2908900.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            14974..15087
                     /locus_tag="PW224_002239"
     CDS             14974..15087
                     /locus_tag="PW224_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2908901.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         5110 a         2555 c         3072 g         4398 t
ORIGIN      
        1 atataattct agttcatttt ttgaactatc agcaaaattg tttttgtctc ttgttttcat
       61 tgatgtgtcg catagttcaa attttgaact atcaacagaa tattgatttg ttccatagtt
      121 caaattttga actatcaagt ttttttgtct attttcttga tgggttatct tattaatttt
      181 ataaacatca tctttgcaca gaacaggctt taacaaataa taaaaatttg ggaacatttg
      241 cattttactt ttattgaagc cattttttct tttcacaaga agtttaactt tttgaagttc
      301 atctagtgct gataagattg tattttttga cattcccgaa atttctacga gatttttgat
      361 ttcaaatatc aaaaaaattt tttgattctc atcatacaag ttatttttca atgaaagtct
      421 atttctatct tttagtaaca tataaactaa cttagaactt agtttcattt tcttatattt
      481 ttcatttacg tttaaaaaaa taggatattg atagtataat tctgcatata tatcagaatt
      541 agaatagcct tgcttttcaa ctaagctttc aacttctttt tcattcattg tcacacctgc
      601 ttctgatttt atttgctata atagattcaa ataaagtttt ctcagaactt tattattgag
      661 tgttcccccg atctgcaaac tgacgggaac actcttttta attttttcta cttcatcaaa
      721 tttaaaaaac gaggtgtttt ttaaatttga tgactcaata tccatttttt gcattcttct
      781 tttgaaaaca actccatttt ttctaataaa ataactggca atcccattct tttaaatttc
      841 aaaaaagttg tatatgatac attcaaatat ttagccatct cttttccttt tagaaatggt
      901 ctttctaatc cggtatcaat tcttgcttga tcaatagcct cttttgtcat ttcaaaaaca
      961 taatttttca attgattcgt ttgttgttta tctaacatta ctgtaattcc tgcatccatt
     1021 atttttctcc tctcattttc tatttcttca atcagccaac tcgccaagtt gtatttattt
     1081 agattttcaa atcattaagt aaccaattta ccaacttttg ataagtagat gctctaatac
     1141 tttctttgct cccattttcg attttgacat atgttttccg atttatacct atcaattttg
     1201 ctgcatcgtt tttagtaagt ataagatcgg ctctatgtct tcttaagctt ctaatgaatt
     1261 gatcattaag agttatcatt cagcgcctcc tttcaaatta ttcactttgt ggtttttatc
     1321 ttaataagca cattttaaac cacaaaacga gtaatatcaa ttaaaatatt actcaatttg
     1381 tggttttcat gctataatag tttggaattt actatagggg gcgttacttt gaatagaata
     1441 aaattttata ttgattccag taaaaagacc attaaacaaa ttagtacaga aactggtatt
     1501 aactatacaa ctcttacgaa ctattaccgt ggtgagcgaa atcctagaaa tactgaaaca
     1561 tggaatatcc ttgctgatta tttccatgtt cctgtaagtt atttgatggg attagacgaa
     1621 acaaattcca tagaacaacc taatcttgat agaataaaaa ctgctcgttt agaaaaaaag
     1681 gacaaaatag tcaagaatat tgccagtctt gactcagatt attttactga attatctaat
     1741 ctatactttt ccatgacttc tttacttgaa gtcaaacttc aagaagatga ctccgacttt
     1801 ctttttttaa aaagtattct agaggactcc tttgaataca cgattctatt tgccaaacaa
     1861 aagaagttta ttaataaagc tttaaactta aatatcgatg aaagaaaaga gttagaatct
     1921 gagttatttg gcaacttaac ccaagtacag gttaacaatc taaaaagaga gataacagct
     1981 cgattaatat caaaacttca ataaaactaa ttattcaatc agccaactcg ccaagttatc
     2041 agattggaga gattagttat gtcaatacaa aagtacacaa caaaaagcgg tgcacgatat
     2101 agtgtccgct tgtatgttcg taaagatgaa aatggaaagc aagaatacta tgtaaaaaaa
     2161 gggtttaaaa ctgaaaaaga agccaagtta cacgaagcaa gaaaaaaggt cgagattgaa
     2221 gataccggct atcataagcc ttctaatgac ttgtttataa cagtgtatga agaatggttg
     2281 ccttcttata ggaatcgtgt acaagaaacg acatatcaac gtacaaaaga ccttttccgc
     2341 ttgcatatac taccaaaatt cgggaataag tccatttcta agattactcc tgttcactgt
     2401 cagaatgcag tcaatgaatg ggctttaaaa ttcaagaata tcaaacagct taagtcttac
     2461 agctctcaaa tatttgagta tgccattttt gcagagctat tacaccgaaa tcctatggca
     2521 aatacaaatc taccaaagcg ggaaaagaaa gaaaatgaaa attacttttc attagaagaa
     2581 ctaaaagctt tttgggatat tctcaataaa gaagagcctt taaaacatat tttaatcttc
     2641 cagcttttga ttactactgg tatacgtaaa ggagagctag ctgctcttca ttggtctgac
     2701 atttattttg aagaacagct aatgtatgtt agaaagagct atgcaactat tcgaaatgaa
     2761 gagaagaatg caagaagaaa aactgtcagg attcaaaaaa atacaaaaaa tatatcttcc
     2821 gaacgaatat taccaattga tgcacaaact attgaaatgt taaaaatctg gaaaaaagaa
     2881 caagctcgag agcttttgca gtttggcata aatacaaata caaaaaatca acttatcttt
     2941 acctatgtaa atgcagacgg tgacattaat caacctttgc atgcggacta ttcaaataat
     3001 atattgaaac gtttagagaa gaaatataaa tttaagcacg ttactattca tggattccgg
     3061 cacactcacg ctactcttct actagaaggt ggagcaagta tcaaagaaac acaagatcga
     3121 ttgggtcata aaaatgctga aactaccctt aacacttatt ctcatgttac tgaaaaagct
     3181 caacgaaatg cgttagaaaa attcattgct tacactggtt tataatattg tcattacctt
     3241 tctcattacc agaacgaaaa aaagacacct tctcgcaatg agaaagtgtc tgaaatcgtt
     3301 ggtataacaa cgattaacgt tttgaaaact gtgaagcttt acgtgctttc ttaagacctg
     3361 gttttttctt cgtatcattg aataagtttg aaaagccttg atttataaag gtttttccgt
     3421 aaatatattt tgttatattt gattaaattt gaaatgtttg tgttctaaaa tgtgttctaa
     3481 tttaaaggca gtatatttcg tattttatat taaattttta ggctcattca ttctcaaatc
     3541 cattctattt agtcaataaa tattttaaaa agactttata aacaaagatg ttcaagcgag
     3601 catcttattt ttttgtgcct aatttgaaag gagaacattt catgtataaa aaacaaaaaa
     3661 agaaattatg tgtattaact attttgagtt tggctgttca atcaattgta ggagcaattg
     3721 ctcctacgat tgcttttgct gatgaaatta cccatccaca aacggtaacc gtagaactag
     3781 atttagcaca tcaatatgct gttgaaggaa cttttagtga tggtcgtcca atgtcagaag
     3841 tcactgttcc gcattatgca gtgtacaacg gcgttaaaca agatattttc tgtgttgaac
     3901 caggagtccc gatttacaat gagttcactc ctggatatga gaagaacccg ttaccagata
     3961 tgtccgataa agccaaatta gtttccgttt tatggaaaaa agtaggtaca gatgcagata
     4021 ctcatattgt ggcacagaag atgatctggc aagaagttaa tgggtatacc cttcactcaa
     4081 taaaacgatc agatggtagt gccgtaaata tcgcagccat tgaggcaaaa atcaatcaag
     4141 ccattgccga ttatcagaag aaaccaagct tccataatag tacggctaaa acagtattag
     4201 ggcaatcgac tactgtgacg gatacgaata atctgaattt gtcagagttc gatgaggttg
     4261 tggaaaatac cgcaaacatt gattatcgtg tgaatggcaa tcagttgata atcagcccaa
     4321 atgccaattc aaaagaaagt ggtgtgttga cgcttaagaa atctgctggt accggaacac
     4381 ctgtcgccta taaaaaggct ggccaacaga ctttaatggc cggggcgatc gataagccga
     4441 acacttatac ggtcaaaatt gatgtggaaa tggagggttc tttgaagatt aaaaaagttg
     4501 ataaagaatc gggtgatatt ataccaggaa cggttttcca tttagacttt ggaaaaactt
     4561 tacccgcaaa agacgtgacc accgataaag aaggcattgc gaccttagat gagattcctc
     4621 atggtacaaa ggtaaccatt actgaaaaat cggtgccggc gccttatacg attgatacca
     4681 cacccatgac tgccacaatt aaagcaggtg aaaccatttc agtgacttca aaaaatacgc
     4741 gagaaaaagg tcaaattatt ctggataaaa ccggaattga aacagggagt gatctttgga
     4801 acgataatta ctctttagcg ggcaatacgt ttgccattcg caaggatagc ccaactggtg
     4861 aaatcgtcca agaaatgacc acggatgaaa aaggtcatgc agaaacacca aaagagattg
     4921 ccaatgcgtt ggaattggga acctactatg tgactgaaac caaggctagt catggctttg
     4981 tgaatacttt caaaccagta aaagtcgaat tgaattatgt caatcaaaca gtggctcttg
     5041 ttaccagtaa cgtaaaaggg caaaaccaag aaattactgg tgaaaccact ttgacaaaag
     5101 aagacaaaga taccggtcat gaaactcaag ggaaagctga gtttaaaaga gctgaataca
     5161 ccttgtttac tgttaaggat ggaaaagcgg tcaaatggac cgagaacttt aaaccagaat
     5221 tgttgaaggg aacgaaagct tctgatgaaa ccgtaacttt gactttagat gaaaagaatc
     5281 aagttgccgt taaacactta gcgattaatg agtattactg gcaagaaacc aaagcgcctg
     5341 aaggatatac cttggatgaa acaaagtatc ctgtatccat caaaaaggtg gatgatagcg
     5401 aaaaaaatgc tgtgattact cgagatgttg cggcaaaaga acaagtgatt cgctttggct
     5461 ttgatttctt taaatttgct ggatcaacta ctggcactgc cgaaactggc ttcaatgatc
     5521 taacctttaa agtgtcgcca ttggaaggaa ccaatgaaat caccggtgcc gaagatgaag
     5581 cgaccacagc ttataacgag caattagggt ttgatggtta tggcaagttt gaaaatcttc
     5641 cttatgggga ttatttactt gaagaagtag aagccccaga aggttttcaa aagattacgc
     5701 cattagaaat ccgttctact tttaaagaaa ataaagagga ttataccaag agtgattata
     5761 tctttactat taccgaaaaa gggcaaaagc aaccgattaa aacggtaacg gtcccttatg
     5821 ggaatctaac caatgagaat ttctcagtca gtttgaaccg tttgatgctt tatgatttgc
     5881 ctgaggaaga agatagctta acttctcttg cgacttggaa agaaggagat aaagaattga
     5941 ctactcttga ttctaccgag ctagtcgata aattgagcta taatctacat gaaatcaaag
     6001 aagactggta tatcgtagct caagccatcg atgtagatgc tacaaaagct gcccaagaaa
     6061 aagataaaaa agccaaaccg gtggcgattg cggaaacaac cgcaacttta gcgaataaag
     6121 agaaaacagg aacttgggag attctgcata aattaactgt cgaacaagtt ttagacaaga
     6181 ccattgtctt attcaactat gtgtatgaaa gcaaagaagc ctatgaagct ggtgatgagc
     6241 cggtagcgaa ggatgctagc ttgaacaatc aagcgcaaac tgttaattgt atggtggaac
     6301 gtcatgtttc catccaaacc aaagcgcact tagaagaagg ttctcagacc tttactcatg
     6361 gtgacgtggt agatatgttt gatgatgtat ccattaccca tgatgtacta gatggctcaa
     6421 aagaagcttt tgaaacaatt ctgtacgctt tatttccaga tggtacaagt aaagaaattt
     6481 ggaaatctgg caagattgat tacgaagtga atgacaaaga atttaccaaa accgtacttg
     6541 cggaaaaagt ggatacaagt aaatatccag aaggtacaag ttttaccttc aaagaaatta
     6601 actatgacaa agatgggaat atcaatggaa aacacaatga agatttgaaa gaaaaatctc
     6661 aaactttaac gcccaaaaaa gttccgacaa caccaagtac accagaacaa tctgaaatgc
     6721 caactgttcc aagtggctct caagaatcta gtcctacagt gaaggcattc cctcaaaccg
     6781 gggagaaaac ttccaatgtg ctactgttca ttggctttag cttgatcttt gcgacggctg
     6841 gctattattt ttggaatcgt cggaactaag gtgataagat gaaacatgta aatagtagaa
     6901 aacaatacgc gcgaccgcct cctaaaaaag tggtcgcgct atttttgtat tcaaaactaa
     6961 caatgaaaag aggaaaaaat atggaattga aatttgttgt accagatatg gtcgagacgt
     7021 tcggaaaact aacatacggt ggtgaaggtg aagttctatc ggaagggtat ggtcgaaata
     7081 ctacaattgt tggtcgaagt taccatctgt tttcaaataa acaacgggcc gatgatattg
     7141 aagtggtggt agctgcggaa gccggcgaaa aggattttga ccaagatcaa ccactaaaag
     7201 ctgtaaatcc ccacttaatt gccaaaggct atgaaattga gaatcgtggg tttaccgatt
     7261 acgtgttgta tgttgatgat ttagtgaaag cataggagga aaagaagatg agattaacag
     7321 aaggtattgt cgtagattca ggattaacct ttggcaagtt gcgtttttcc gcattacgtc
     7381 gagaagtacg caagcaaaat gaggacggaa cggttagtaa cgaggtaaaa gaacggacct
     7441 ataatttgaa atcctccgcg caaggtcgaa tgatccaagt cagtattcca gcaaatgtac
     7501 ccttacgcga gtttgcttat gatgcagagg tagagctggt caatccaatc gtggatacgg
     7561 tcgccaatta tgtttttcgg gaaggaacca ccgttaattg gtttatcaag gcagatgatt
     7621 tagtcttgaa acgacaactg aatcaaggaa aacctacgaa ccaaaatgaa ggaaagaaat
     7681 aggtgaagaa tatggaaatt aatgttgaac atattttaga ttgtctcgat caatttggca
     7741 aaggtgagct aacggaggaa caactcacta acgcattaac ctatgatgaa aaaatgtttc
     7801 tgattatgca tcaagggtta ctggaagtag gtaacgatgc caaagaagac tttgatgtac
     7861 tggattggtt aggggaagaa gagtctttct ttatggttgt cgaggtaaat gaaagtttat
     7921 gtcggcaggc agaatctgtt ttggaagaaa tcgatgtgga gatgccggat gccatcgaaa
     7981 gatttttaaa acaattagtg gaaacgaaac aacttcccgt agtagtaaat gactagcata
     8041 actttttgaa attgcccaat gaaatcctat tgggcttttt cgactggagg tggaaaccat
     8101 caaaatgtac aaaggacatc gcatacgcgc aggagatcaa cacttgattt atcactttgt
     8161 tcttggctgg ctaatctttt tgttcattgg ttggatgagc gtcttttatt tacaagaact
     8221 tagacaattt gatatttcta aggtatcatt ttccactagt gaaattggct ggtctatgaa
     8281 ggatgtaatc tgcttgctag gaagtctcgc tctttcagga agcatgatgt tactttatat
     8341 acactttttt caggatcatt ggcgaagtct ttggcatcga caaaagttag ctcggatgat
     8401 tctagaaaac cattggtatg aagtaaagca gactcaaggt gaaggttttt tcaaagattt
     8461 gaatagtagt tggaccaaag agagtatcag ttacttcccc aaaatttatt atcgaatgaa
     8521 agatggctta ctttctattc gcgttcaaat ttcactggga aaatatcagg atcagctatt
     8581 gaagttggaa aataagttag aaagcggact gtattgtgag ttagtagaaa aagaactcaa
     8641 agactcctat gtggaataca ccttgttata cgatatgatt gccaatcgaa ttgggataga
     8701 agaaatagtg gcagaaaatg gggcgttgcg attgatgaaa aatcaagtat gggcgtatga
     8761 ttccttgccg catatgttaa ttgctggcgg cacaggtggt gggaagacct atttcttact
     8821 caccattatt caagcactat tgaagtcaga tgcagaattg tttatcctcg atcccaaaaa
     8881 tgcggacctc gccgatttag gtacgataat gcctcacgta tactcccaaa aagaagaaat
     8941 ttctgaatgt gtggaagatt tttacgaacg catgatagct cgtagtaggt caatgaaaga
     9001 aatgcccaac tacaaaacag gagagaatta tgcgtatctc ggacttccgc ccaacttttt
     9061 aatctttgat gaatacgtgg cttatatggg tgcgaatcgt tttcctacaa gcatcgaata
     9121 gatgtgaaat ggaagcctat tgagattatc aatagtgaca actaatgttt tgagagatgg
     9181 aatgaaagcc ttcaagttga gctgaaacat ccccgctaat actcctacat gctctaatca
     9241 atgaagtaga ttggttttgg tatgaagcta ggtgaagtcg tctgaaatct gcctaagtgc
     9301 ttgagcataa ggctgactat agggcgggcg gctgaaaaag ataactatgg taagaatgtc
     9361 tgaaaatgac tgacgaatct gcgaatgtac gggtctaaaa ggctgatctt attgaaagat
     9421 aggaacatac ggacgtatgg tgagataccg acaagtaagg ttccatgata tgaaaatcgg
     9481 gatagctaac gaagaagcta taatttcaca ggctcatagt agatacctaa ggttatacat
     9541 gatagctaaa aacattggaa cgatgtaagc agaaaactat caattaaatg actacttcta
     9601 gataggtgca tataagtaat aacgaaattg ctttgtttct gtgaaagtaa gggcacagta
     9661 ccgatgaagc gtctaacaaa tgtggaggga tagccctaag tctatttaat aacaatgaat
     9721 gttgaaacag atgaagttca aggatctggt aagaacgtgg gaaaatccgt tatggaggtg
     9781 ccacgcttga acacaaaaat acgttactgg gaatattatg atctacaaga aacattcgat
     9841 cagctattta ctcaaagtag aaacgggaaa aagttttatc aattatatga gttaatcatt
     9901 tctgaaaata atattctttt agcataccgt actatcaagg caaacaaggg gtcttcgaca
     9961 ccaggaacgg attccttcac aattgataac tataaggaaa tgaaccaagc agagtttatc
    10021 catcttattc tcagtcattt agaaaattac aaaccaaaat ccataaagcg agtaatgata
    10081 cctaaaccca atggtgagaa acgcccatta ggaattccat gcatgattga tcgaatgatc
    10141 caacagatgt tcaagcagat actggagcca atatgtgaag ctaagtttta tgaacatagc
    10201 tacggtttca gaccattgag aagtgcaaag catgctctag gtcgcattat gtatctcata
    10261 aacatcagta aaatgcacta tgcagtagac attgatatca aaggtttctt tgataatgta
    10321 aaccaccgtt tactgataaa acaattatgg aatataggaa tttgtgacaa acaagtctta
    10381 gctattcttt caaaatctct gaaatcaccc attcaaggag aaggtatccc gagcaaagga
    10441 acgatccaag gaggtatcat ttctccacta ttatctaatg ttgttctaaa cgacttagac
    10501 cattgggtct caaagcagtg gcataccttt gaaacaaagt atccctatac taaaggttac
    10561 aataaatttc gtgctctaag agatacaaat ctaaagcaag gctatattgt caggtacgca
    10621 gatgacttta aaatcatgac taatgattat ccgacagcat taaaatggtt ccacgctgtc
    10681 aagctttacc taaaagacag acttaaatta gatatttcca atgagaagtc taaaatcgtc
    10741 aatttgcgta aatgtaagtc ggaattttta ggattcgcta tttgcgtgaa acaaaaaggg
    10801 aaaaaatggg tttgtaattc acgtatttct aacaaaaaaa aggatcagat taaagaagag
    10861 attaaacaaa gaatcaaaga tattcaaaag agtcctacag cacaaaatgc tctcctgttt
    10921 aatgctctcg tgctcggtgt tcataattat ttcaggtatg ccactcatat taatttagat
    10981 ttgaatagga tgaactacgt tctttcattc actacttaca accgattatc aaattgtagt
    11041 acaagatgtt accccaacaa agcttcccca acttacaaaa agttttactc aacaacgatg
    11101 aaaacgtata aagtagctgg agtctatttg taccctcttt gcgacgtatc tacaaaaaca
    11161 atttacggat ttaatacgga ggatacaccg tttactccgt ttggtcgtca aagattggag
    11221 cacaaaagcc tacagtctct tgtgttccaa gaattaagaa aattgatgga atcgaaaata
    11281 ccgaatagaa cggttgagta tttagatagt agaatatctc gttactcaat gaaatcaggc
    11341 aagtgtgaga ttacaaaaca atttttgcca gcaaaagccg ttcactgtca tcactatttg
    11401 ccaaaaagtc ttggtggaga cgacaaattc gataacttga gaattattca taaggatatc
    11461 catcttctga tccatacaac aaataaaatg ataatcgatc actacgtgca tgaactgaaa
    11521 ctggtgccag agcaaatagc aaaaatcaac ctttatcgaa agatgtgtaa tctgcaaaac
    11581 attcaataga aaataaatag atggaacgcc gtatgaggtg aaagtctcac gtacggtgtg
    11641 gagcggggga aaagatggag attatatcaa attcttacct atcgctatag atgttaacga
    11701 caaaagaaag tgcggtgatt ttgaataagc ttaaacaaat cgtgatgtta ggccgtcaat
    11761 ctggtttctt tctgattcta gcttgtcaaa ggccggatgc gaaatatcta ggagatggga
    11821 ttcgcgatca gtttaacttc cgtgtggctt tgggtcgtat gagtgagctt gggtattcca
    11881 tgatgtttgg agaagtcgat aaaaattttt ttatgaaacg catcaaagga cgaggttatg
    11941 tagatacagg gggcagtgtg attagtgagt tttatacgcc acttgtacca aaagggtatg
    12001 atttcttaag ggaaattagc aacattgttg gattaaccgt tcatacggaa agagaaaata
    12061 atagtgtgta gcctaggaat atcaataatc ctgggctttt ttattgaggt gatttatgac
    12121 atttttagat ttattcgccg ggattggtgg ctttcgctta ggtatggaac aagccggtca
    12181 ccagtgtatc ggtttttgtg aaattgatga atttgctcgg aggagttata aagcaatcca
    12241 tgatacaagg aaggaggtgg aaatgcatga catcacaagt gtatcagacg aatttgttcg
    12301 atccctcgga ccagtggaca tcctttgcgg cggctttccg tgccaagctt tttcaattgc
    12361 gggaaagcgg caaggatttt ccgatactcg aggtacttta ttctttgaaa tcgctcggtt
    12421 cgccactctt ctccaaccta agtttctatt ccttgagaac gtccggggat tgctcaatca
    12481 cgaaggaggg gctacgttcg agacgatcct ccgaacgttg gatggattgg ggtatgatgt
    12541 ggaatggcaa gtgcttaact caaaggccta cgttccccaa agccgtgagc ggatcttcct
    12601 tatcggacat tctcgagatg catgtaccga acaagtattt cctatcattg gatcgtcttc
    12661 aacatctgat caaaacatca gaaacttggt aaatatcaat ccttccaatc gaggaatggg
    12721 tggccaagtc tatggttcag atagcgtagc accaaccttg accggtgatg aaggaattaa
    12781 aattgcctta ccggtaaagg atgggatttc tgtagcgggt atgttgtcgg gaaattttga
    12841 acaaggcaat aggatttatg aggtaaccgg aacagccccc actttgtcaa cgaaacaagg
    12901 gggaacgaag attatgattc ggaaaaatga aacagaatat caagaggtga agccagggga
    12961 cagtgtgaac ctggcttttc ctaattccac aagtcgccga ggtcgtctgg gaaaacaaag
    13021 tgtgcatacc ttactaacag gagaccaaca agcggtagtc acggatcagt accagattcg
    13081 aaagttaaca cctagggagt gctggcggct tcagggcttt ccagattggg cttttgatcg
    13141 agcttctcaa gtcaattcag atagtcagtt gtataaacag gccggaaact cagtaacagt
    13201 tccggtcatt tttgatattg ctagaagatt aaagggggta aaagcatatg atttatgacg
    13261 agttgattgg agaaatctac tggatgattg gcaagattca ggcagatccg aaattggaag
    13321 aagaactgta ccggctcaat tttgatattc gcaaaaacgg ggtgaaagtc caggtgattc
    13381 gtatgtagtg gatgaggaaa cggatgctcg aatggagctc aatcaagtca tcggtgaatt
    13441 ggaacgaatt gcggaccttg ccaaagaacc tgatatccgc caatacttgt ttgagataaa
    13501 agcagagttg gaaattgagg gtatcaccgc ggagtaatag ggaaataacc cactgatagg
    13561 cactgaccgc gggggatttg cgaagcagaa atcctccgtg gtgctgtgcc tgttcatgga
    13621 cggcggcagc cggccatgat gtctcacccc ccgtatctaa caggggggta caaaaacaca
    13681 aaaaacaagc aagtaaccat aaaaaatgct tgttgtatca aggatattaa gtaatttgta
    13741 taggtgttaa ctcatttgga aaagttaaca ccttgaattt ggaggtgaaa acttggcgca
    13801 acgaaattta gattatcggt tattaaaaga acgacgcaac gaatatggcg tttcacaaaa
    13861 taaattagct actgcttgcg gacttagccg cccataccta aatcagatag aaaacggtgg
    13921 cgtaatcgcc tctacaaaga ccatgagaaa aatttttgat caactggaaa gtttcaatcc
    13981 cgatttacct ttaacgttac tatttgacta tgtaaggatt cgctttccca caacggatgt
    14041 gcggaaaatt attcaagaaa ttctccattt gaaattcgag tatatgcttc atgaagatta
    14101 cgccttttac tcctatcaag aacaatatgt catgggcgat attgtagtga tgttatctca
    14161 tgaagaagat aaaggtgtcc ttttggaatt aaaaggtcgt ggttgccggc agtttgaaac
    14221 ttttttactc gcccaaaagc gtagctggta cgactttttt gaagattgtc taaaagccgg
    14281 tggcgtgatg aaacgcttgg atttagcaat caatgatcga gtggggctac tggatattcc
    14341 tgatttaacg aaaaaatgtc agaaggaaga atgtatctcc ttgtttcgta ccttcaaaag
    14401 ttaccgttcc ggggaactgt tgaaagctga tgaaaaggat ggtatgggca ataccttata
    14461 tattggcagc ctcaaaagcg aggtttattt ttgtttgtac gaaaaagatt atgaacaata
    14521 tatcaagtta gggattccat tggaccagac cgaaacgaaa aatcgttttg agattcggtt
    14581 gaaaaatgac cgtgcttatt atgcgattca agatttgtta aaaggtcgta gcattgaaag
    14641 taccacgttt tccattatca atcggtattt gcgatttgcg gataaagtag atggaaaacg
    14701 gcgaaccaat tggcccttga atgaacaatg gggtcgtttt attggacgga atcggaaaga
    14761 gattcaatta acttctgaac cgaaaccgta taccattgaa cgaacattaa attggctagg
    14821 tagacaagtc gcccctacgt ggaaaatggc taaggagttg gatcgattga atcaaacaac
    14881 gtatatccag gatatggtac agaatgcgcg actctcggac cggcataaaa agattttgga
    14941 gcaacaaagt atggcaatcg aaaacttgat tgtatgaaaa gaggaatttt gatggaaatt
    15001 gtaaaagata tgttattggt acttggtggc agtatgcttg gtgttaccgt gatgtgcttg
    15061 atgaatgcaa gtgcagcagc ggattgagca atggaaaaga aaggaaagtg acaaaatgaa
    15121 tttcggacaa aattt
//
LOCUS       DANKMN010000071        15095 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000071 DANKMN010000000
VERSION     DANKMN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="PW224_002240"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="PW224_002240"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL2908902.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="PW224_002241"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="PW224_002241"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL2908903.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3872)
                     /gene="iolC"
                     /locus_tag="PW224_002242"
                     /pseudo
     CDS             complement(2837..3872)
                     /gene="iolC"
                     /locus_tag="PW224_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
     gene            complement(4029..5045)
                     /locus_tag="PW224_002243"
     CDS             complement(4029..5045)
                     /locus_tag="PW224_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2908904.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5147..5344)
                     /locus_tag="PW224_002244"
     CDS             complement(5147..5344)
                     /locus_tag="PW224_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908905.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5316..6635)
                     /locus_tag="PW224_002245"
     CDS             complement(5316..6635)
                     /locus_tag="PW224_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL2908906.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7376..8356)
                     /locus_tag="PW224_002246"
     CDS             complement(7376..8356)
                     /locus_tag="PW224_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2908907.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8369..9247)
                     /locus_tag="PW224_002247"
     CDS             complement(8369..9247)
                     /locus_tag="PW224_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2908908.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9263..10063)
                     /locus_tag="PW224_002248"
     CDS             complement(9263..10063)
                     /locus_tag="PW224_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDL2908909.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(10338..10487)
                     /locus_tag="PW224_002249"
     CDS             complement(10338..10487)
                     /locus_tag="PW224_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908910.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            11455..11781
                     /locus_tag="PW224_002250"
     CDS             11455..11781
                     /locus_tag="PW224_002250"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2908911.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            11762..12697
                     /locus_tag="PW224_002251"
     CDS             11762..12697
                     /locus_tag="PW224_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2908912.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            13326..13577
                     /locus_tag="PW224_002252"
     CDS             13326..13577
                     /locus_tag="PW224_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HDL2908913.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            14119..14277
                     /locus_tag="PW224_002253"
     CDS             14119..14277
                     /locus_tag="PW224_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908914.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            14349..>15095
                     /locus_tag="PW224_002254"
     CDS             14349..>15095
                     /locus_tag="PW224_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2908915.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDF"
BASE COUNT         4389 a         3252 c         2559 g         4895 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa agcagcgctt
     3181 tcattatctg acatgttagg ctggatcaac ttctcagtta gatcgaactc ttctcttgaa
     3241 cccatgataa tgtctgattc cttagcaatc atcgaatagt aaattgaaat atcatcgttg
     3301 ttcttccagt tataagcacg gtaatcaata tcaaaaataa ttttgacatt attccttttc
     3361 gccagcatca ctgccttgat tacggcttct ctggacgggc tctctgctag tgcagttcct
     3421 gagattaata gtgcttttga ccgtttcaag tagtcctcat caatatcctc aacgctaagc
     3481 tgcaggtctg cggcttgatt tcgatacatc aaaatattac tttcctttga tgagagcatt
     3541 tcggtaaaag tcagccccag cttttcacca tgctgcgctt tgctgatctg agacgtatcg
     3601 atgccttgtt cattgaaata atcgatcaca tattcgccaa actgatcgtc cgagactgtt
     3661 ccgataaaac cagctttcat gccatgttta gtaacaccca cagcaatatt ggcaggagat
     3721 ccgcccacaa atttttcaaa catatggacc tttttcaatg gtttaaactc ttctttgact
     3781 tgatcattgt aggcaggatt aaaatcaata gcgactcttc ctaaaagcag caagtcaaat
     3841 tcttttgatt ggtcaaactt tatataactc aatatatcca cctctttatc cgttcttcta
     3901 tttctgaggc aagtatagca cgccaaaaca aagcgtgcaa tatttatttt agaacgcaat
     3961 attgcgtgct gtttgaaaat gacggcaaaa aaaacgtgaa tcgccttaca tcacgattca
     4021 cgcctagatt aaatattgta ttttgtctta ataattatat cagtaaatag cgcatctttt
     4081 gtaaccgttt tattattttg cagctgattc gctaacatca gtaatgaacg atagccttga
     4141 acaaatccat cttgatcgat tagaaaatca acctgatcgt ttttcagcat ctcgatattg
     4201 ttcttagtca aatcataaat gattacaaag ggccgcttgc tttcatcgat tttttccaac
     4261 gctcgggcaa ttcccgcttg tccgccagaa aagacgacta cgccatctaa gtttgaataa
     4321 ttctccaagg tgttgcttaa aatcttttca acctcagcac tcgaatcaaa gctgctctga
     4381 acacccgtca gctccagttc aggaaacgac tctttcaatt cttcaacaaa gcctgccacc
     4441 cgtaagctgt tgacgctatt tccaaagtag ccggtaatcg ctagaacctt gccctttcct
     4501 tttgtcagca tacctaacaa tcccgcagcc gtttttccgc ttttgaagtt gtccatccca
     4561 acaaaacact gtcgattcgt accaagaata tccgaattaa aggtaatgat gaggatgcct
     4621 tgatcggtca attgattaat tttttctctg ataccatcac tatctactgg catgatcgcg
     4681 atccctttta ctccaagctc caccaatcga tccaacgcct gcagctgggc cgcttcatcg
     4741 accccgtcta cctcttctaa aaaacaatca atgtttcgct tttttaaatc cccgataata
     4801 gattccagac ctgatcggat cggaatcata aaagaagatt tcgccaattg agtgacaatc
     4861 cccagcttga gcgttgtctc ttttatgacc ttctttttct ttggaacata gttcatttcc
     4921 ttggcgattg ctctgattct ttctgcaacc tcaggattga tccttcctcg atcgttcaat
     4981 gcccgatcca ctgtccccct tgacactcct gctttctcag ctatttcttt aattgtaccc
     5041 gccatcttgc ctctcctttt ttcctattgt gtttatttta tcacaaaact taagaaaacc
     5101 accggagaat gaattctccg gtggactttt tgtatgatta accttcttat tgaacatcac
     5161 tgatcttaaa ctctattcca agggcgtttc tataatattc tggatacatc tccttcccac
     5221 atttttcaca ggtaaattgg ggaggattgt tcggatcatt tgcaatatct gcatcatcta
     5281 aaaattttac tacatccatt gggattgatt ctttttcacc acaacttaga catataaatt
     5341 tcacgttggc gttcccaatc ccagcgaatt gctttaaaag ttttttcttt ttcttcttca
     5401 tactgttgcc tgaattcttt ttggtcaatt tttcgattct cctctcgcat aaaccgcttt
     5461 gcatggttat ctttcgcata tttaataact aaccgtccat ttttgcgcac actcatccca
     5521 acgaattcat agtactcacc gcaacaagaa cattctaagg gattttcacc aaattcttcg
     5581 gttattcttt cttcccagga ttttggtctt aaggcctcat tcagattgat aaacagcttt
     5641 tttactcgtt tctgatactc cttcatgacc tttttcatta atgttttgat tctacgacta
     5701 tagattccat agcgacgaat cgttttgaaa ttcttatttt gaatatggcg aatcagtgca
     5761 gctatgaatt cgtctatact catggtcatc gtctctttag tattcgttcg tttgtccatg
     5821 tagttgaaca tgacagtgtc gccatcatac attaagattc ggcttaatgc gatcgggcca
     5881 cgcttcatat aacgactgat gtattggagt agtgacttta gatttgtcct gctcctttta
     5941 ggagcattta cgtaaaaccc ttctccattg ttcttgtacg cagcttgtaa tcttgattga
     6001 actctttgct tgtcccactc acctaacgtt cgtctaatta gtttcaatac agcatttttc
     6061 cagtattttc ttaacataga aaaaggtaaa taatcgtatt cttcccaact tccatctctt
     6121 gttaagccac ccatagtaac aaccatatga acatgcggat taaattctag ttttgatcca
     6181 aaggtgtgga ttgtagctac tatacctgcc ttcacctttc ttttctcaaa ataggcaaga
     6241 ataatttttg cggcttcatc cattagtcct ttcagtaagg aatctcgata gttttccatc
     6301 ataaagattt cccgtaaccc ctcatcgatc gtaaagatta cgtgccgatg tgtcacttta
     6361 aacatatcat tcgccacaac cgctgaccac ctttggcttt cacctgttgc acacgttgga
     6421 cagaactttc cacgacattt caagggaaca aactttgttt gatgacatcc ctcgcagaca
     6481 aacagtcggt atcctttttg aatatctcca caatatctaa acttgtttac ttcccgtttg
     6541 acgactagcc gaatctttcg cccgtatttc tcaacaaagt tatcccaatt atggtgttcg
     6601 tcaaaaaaga ttgcttcgag aatattcgct ttcatagtaa tattttactt gttcaaaaac
     6661 aaaatgaaaa gggctaaaag actggtagtg cagccttttt agcccttaac tttttatact
     6721 ttctttactg tctaaaaaat atgcggcgca cagaacgcgc cgcatatcgg actaagtttt
     6781 attcaaattt gtcttcgcca acacaatttg acgaagagcc ttttctttca ggaagacttc
     6841 ggtaattttt aggagttcta cctattcttt taaccgttta ttctcttttt ctaagcgtgt
     6901 aatttcattt ctgcgaatgg atttcatgac ttcctctacc gagaaaagta cgttatccat
     6961 atttttcaac ttcttgaact catcaataca cgctattaga gcgagcttct agctatagac
     7021 taacttcttt aacagaaaac ccttcttccg ttaccagttt tacagcggta attttaaata
     7081 tttcatcaaa tgaacgtctt ggcataatcc agatcttcgc tttctcttaa tttggtgtca
     7141 attttagtat aacccgtcca acttattttt taactaagtg cccggttttt cttgacaact
     7201 ccacccaaaa gttagatttt ttggtctaag ttttagggtc gcttcattac tagaattcaa
     7261 caagagatgt tttttatttt atttaaatta tggggtcagt ttccatttga ttacaaactt
     7321 ctttgttttc tttttttaaa cgcaataata aaggttttaa tttcagcctt tttaattaga
     7381 aaatcaattt tcccaaatta ttttctttag ctttcttaac gatttctgtc gcaactacca
     7441 catctaatac agccgaacca actgttttga agatcgtgat ttgttctcca cttgtccgac
     7501 cgatcaattc ttcattgatc agttggccta gttccccatg atagctgtct ctttgaatat
     7561 acccatcttg cagcggtgaa atgaagtccc cagcttcttt tagaacaccg tccatggtat
     7621 caaaaataac aacatctgcg gctttgatta tttctcgtgg aatttcacac atttccggtg
     7681 tgtaagcacc gactccattg atgtgcgcac cttttttgac ccactcagca gagaatgtcg
     7741 cacgttttga agtggtcaca cttgtaatga tgtctgctcc ttcaacgcat tcttgactgg
     7801 tttttgttgg gcgcatcgtt acagaaaatt gttgcatcat tttttcagca aattggctgg
     7861 cacgctcaaa atcaatatca aaaatccgaa cttcttctaa tttacgaacg gtcaacattg
     7921 cttctaattg tgactgtgct tgtccgccgg ttccaatcag cgcgccaatt ttagcatctt
     7981 ctcttgcaag aagttctgtt gctgctcctt gaacagcgcc tgttcttaat tgtgtcaaat
     8041 aagtaccatc caaacaagcg gataccatac ccgtttctgg atccaatacg atcatcgttg
     8101 ctggtacact tggtaagttt ttcttgatgt tttcaggata cacggaaacg attttgacac
     8161 ctagcgcgcc ttcgccacct tctacataag caggcatata taagctttgt ccgttgcttt
     8221 ttggcacatc gatattggtg cgaagaggca cacttgcctt tcctgctgaa tacaatgaca
     8281 gtgctttttt gtccgcatcg atggcttctc tcattgaaaa acttttgata atatcttctt
     8341 ttttaagaaa taacatttga atcgctcctt aattagctgc tgtaactttt tttaattgac
     8401 tgcccaacat cgtatatgca ctgtatagta agaccaaaat cacgatccat ttcaggatat
     8461 aggtatctaa agatttgact aggaatacag cgaccaatac ccccaataca ccgaaggttg
     8521 aagtaaaaag tgtgattttt ctgctgtatt cattaaattt aatgaattga acactgccga
     8581 tcggtactga gaaggtacaa gcccccatca taataggaaa ggctgcaact ggattcaagc
     8641 ctagcaagta gacagttacc atcgttaacg cataagaacc aatgccgata ttgttcaatg
     8701 caccatagac aaagagtaaa aatcctgcca agattaattt tccgccatac aattcactgg
     8761 ctgttccgtt tgatggaatc cagttcattt gtcctgcaaa aatcaacaat gcagcaatga
     8821 ccaatcctat gccgacaaac agtttgatcg ttttctctgg taacttcacg acaaatcttg
     8881 gtcctaaata tgcaccgata atttggcaga taatacaaac agctagtgtc ttgatcccta
     8941 cgttgatggc agtgatgtaa gacaatgcca tgacggctac cggtactaca cattgggtat
     9001 tcaacgttcc gggtagtttt ttcatgctga cccatttcaa ctttggataa aggacggttc
     9061 cgatagcaaa gtcggaaacg ccaaatgttg aaagaaagaa gatgataaat gacgtgaagg
     9121 gcaacagacg catatcccct ggttcttctt tgaaattttg tttgtttttg aaaaggtctc
     9181 ttagaaagta aataataaaa tatagattga ttgcgacaat tagtgtcagc aaaatattcc
     9241 gaatcataaa aaattcctcc tactagtaaa tctttctgat tccctcaaag ccctcaccat
     9301 attttgcttt aagttcttca ggagattctg caatcacttt tagtgtatat gcatcatgtt
     9361 tgacatattc ttttgctaga ttgaccatgg cgtggttttt tgtccaagtg acatcggttg
     9421 attgcagttc ttcgttaatg gtaccaaaca cgacttcttg ctcatcggcg acgatgttga
     9481 tccatcttgc tccaaagtca gcaagtttgt ctgtttcaaa accatgaata taatactttt
     9541 ctaagggaag atcatattca tgggtagaac tgaataaaat cagtacaaac ttatcaactc
     9601 ttttttcagc ttgctgcaat gcctttagta gtgaatccgt gaaattctca tgccaaactt
     9661 gaatgagcaa actttctttt gcgtttttca ccaaatgctc tactttatcc agaacatact
     9721 cgataccatc aactttccac agcatttctt cttcatcttt ttctttgatc atccctagat
     9781 tcttggtaag aaaagaaaga tcatttttaa cacttttttc taatttctct aaaaactcat
     9841 ttgccgagat ggcatgataa agtttaggtt ctgatttatt aacgacaatc aaattctttt
     9901 ttaatagttt ttccaaaata ttatatactt tcgagcgtgg cacacctgag attttactta
     9961 cttcatatcc tgtaagatta cctttttcta atagtgtggt atacactctt gtctccatct
    10021 ctgaaaaatc atattttttc atgatttgaa taatcgtttc cattgattaa tcctcacttt
    10081 atttttagta gctactacgg taacaactat aaattctttt tgattatctg tcaagagtta
    10141 gttcaataac acacatataa taattagctt aaaaacaatc tttttattaa aaagaaacat
    10201 tataatatga atgtttgtga aaaaataata caacttagtc attctttaca acgatttata
    10261 gaaaaacaac ctctttaaat ataaaaggta cgggttactg attacttgta aatctttacg
    10321 ttccagtttt attctttcta aaaaatatac tcacttcttt tcttagactt ctttttttga
    10381 ttctcctctt tatggtcttg tccttttttg ccttcaaaat atttaaataa acccctttca
    10441 aaagcatttc taggttttgg acaataagga atgacttttt tcttcatata aatacctcat
    10501 ttaactattt gatatcatca cattatcaaa aaaaataggt tttttattaa aactatcttg
    10561 acctttcggg agatttcact gtgatttatt tttattccca tacttttttc gttggtcact
    10621 ttcgtatacc taatctaagc gatcatcggc gacggtgctt ttacaacgaa tggcaggaca
    10681 aaacaagggg cggaaaaact tttttgccta tttcataaaa aactttttcc tacagcaatc
    10741 ccttgttttg tcctaccaca cgttgtgaac ccaaagcacg ccgacgaacg cttagctata
    10801 cgaaaatgac ctccaaaaac aaaaaagtga ggtgaataga aacaaaaaga aattgaaatc
    10861 tcccaaaatg tcgtccgcct cggagacaac ttgctgctga aaatggttgg ttaattcatt
    10921 taagtattaa ataagataaa tcatcaaatt aatcggaaaa atagcatgat tggccttatc
    10981 aaaaataaac tgtaaaaact tatatttcta tgagtggtgg aacaatgcta ataccaaatt
    11041 aatttatcga ttacccacct gcacaaacca aagatagcat tattaggaaa cgaatcaatc
    11101 agcgaatcca gcattataat gaggatacta aaaataaagt tttcataatt ataaatccgc
    11161 cttcctacga aaggcaccaa tgaggtgatg aaaaccttag agcgctttct ttcttttttt
    11221 attccgtgtt accctttcct tgcaggtccc tggtacacta cagaacccga ggaggtgagt
    11281 ggcggggctg tatagcggca accccgcata attatatggt gtcggtcatc cgttaaggca
    11341 tcaatgaatg gcgcaaaccc gtagcccgta aacttatctc ttccgaagtt gactcgattt
    11401 cgccggatga ccagtcccta ccaaacctgt actatttttt tacaggaggt tgctatgttc
    11461 aaaatctttc gtaaaaattg ttgtggcttg gatgtccaca aaacgtggat ctatgcgtgt
    11521 atcggaatca cagatgcgaa tggtcgtaca gtgtataagc aagctcgctt ctcttccttt
    11581 acaaaaggtc tcaatgaatt agctgaatgg cttgctaaat acaactgtaa ggatgtgtgt
    11641 ttggaatctt ccggtaaatt ttggattcct gtttttaata ttcttgaaaa aacttgtttt
    11701 gtcacactgg ctcatccgaa gtacaccaaa ccccagaaag gaaataaaac agatcgcaaa
    11761 gatgcaaaaa tggatttgtg atctctttat gtgcgacatg atcaaaccca gttttattcc
    11821 tggtcttgaa atccgccagc ttcgtgatct aatgcgttat cggacgaaac tgaccaacat
    11881 gaaaacaagt gaaaagaatc gtgctttaaa ctgcttaacc gtttctaatc tgaaactaga
    11941 tgatgttttt agtgatgtgt tcggaaaatc atccacttct atcatccaat acttgttaga
    12001 acatcccggt gaatcatttg atgttcgacc atttatccat gcccgttgta aaactcccgt
    12061 tgaagaaatc caagcggcag ttgagggggc gatttctgta cctcaagcga ttaagcttcg
    12121 ccaatgcctc aaccatatta acgagattga gaagcatcga gaagagatca atctagagat
    12181 tcaagctctt gcagaaccct tctcccctgt tttggaacta cttcgtacca taccgggctt
    12241 agacactcag ccaataacag cgattgcgat tctttcagaa ataggaccgg atatgtccgt
    12301 gtttccaact tcgaaacact ttatttcttg ggctggatgc tgtccaagaa acgatcgcag
    12361 tgcccgacga acaaaatcaa cacgtatctc tcgtgcaggg cagtcgttga aaccgctact
    12421 tgtacagatt gccaatgctc ttctcagatc caaagaacat ccagaattta aaaatcgcta
    12481 tcgtagaatc aaagcgaatc gtggacacaa gaaagctatc attgctattt gtaagatgct
    12541 cctgaccgct atctggaatg tcttatcaaa ggtggaaccc tattctgcta aaggatatct
    12601 ggagccaaaa cctgcgaaag aagagaaagt tctgacgcaa tcccaagctc tggacctttt
    12661 aagaaaacgc ggatatacca ttatagatag tgcttaaacc aaagttacgt tgattcttcc
    12721 taaatagact cgttgtcttc catttcaaaa attttgaaaa cggtaggctt atttagtgta
    12781 ccgttcttca ggtatcttca aatagattta ttttcaaact tatttccttt cttcttttac
    12841 tctcctatag cttagcattt aagtgggcac tcgtgtcacc ttgctagata gctatgaatg
    12901 ccgtaataag cctcctagta tataggcaga ctgccaatta taactgattc aatccacatt
    12961 atgataaaat ccaccttttc gtttcatatg tgttgaaata tcacatatgt caaaagtgca
    13021 cgagtctttt cattcagtgc tttgtaaata ctcaaaatta ttccttttca ctttaaaaaa
    13081 aagataatta gtacaaaatc agaaatgagg aaagtattag gatggactag aaaggagttg
    13141 ttgaatttgt tgcaacagat tggtcattta tcaattaaaa attaggtgaa gttttattca
    13201 tcactgcagc tgctgtttcc cagagaataa aacgtctaga agaaactggt cagttactgt
    13261 attatcaagc agcaattgac tacgaacaag cttgattact aaccaaagcg tttatccacc
    13321 tgttgattaa atcaaatcaa aaatcaggtt tttatacgtt cataaaagca atctccagtg
    13381 tactcgaatg tgactgtatc ataggtcctt attcgatgtt aatcaaaatg gggtttcctt
    13441 ctaccgaatc attggacagt tttatcaatg atttatcaaa gtttggacga accaacaccc
    13501 aaatcgtttt ctcgactctt gtttccttaa tggtttatca gttctggaat gtttatcatg
    13561 cgcttttatt aaaatagaaa agagcttttt tcatgcgaca aagcagtgat catttattgg
    13621 cacatgcaat ctttgtggat ccattttttg aaaatacgat atttagtttt gacccaaaaa
    13681 tctttaaata cgtatttgat aatttatctt aacaaccaga taattttatt cagcaccgta
    13741 ttatctatat tgaattctcg gaatcaagat aaattttttt ccgtttttgt ttctttttta
    13801 atcttcttct tgttattaat agtgattagg atgatattcc tatattgatc tttgataatt
    13861 agatataatt tttgaataac gttccctgct attcgagcta gaaatacaag tacgccatga
    13921 cctagttagc gacaactact tagagatttc ttttgttctg ggacaacaaa atgccatgac
    13981 aatagcagta aataatgata cttccgtaag gctcgccgta aactggggag aggtgaaatt
    14041 cctatgtgct tacccaaagc ggttcaaaaa ttttaaacta ttattttagg agatgatttt
    14101 ataaaatcga aagcctactt gaataaaaaa caagcctgtg aatttctagg aattgataat
    14161 aatactttag ataagtggat tcgtgtatat aattttccag taataaaaat tgatggcgta
    14221 gttcgctttg acgtagagga tatcaaagat ttcatgaata cattcaaaaa aaattgaaat
    14281 tttcttctcg tcacatatac tataaatagc gaaatgtact gccttacttt ttaaaggagt
    14341 gaggaagttt gcctacaaag ctttcaaatg ggaaatataa aactaatctg aggtatccaa
    14401 aaaaattcag agaaatcact ggaattgcat cagagaagta tcaaaaagta tttccaacta
    14461 ggcaattagc tattaaggcc gaaagggaaa tgaaaaagaa gattgaaaca gttttaagag
    14521 aggagaatgc aaactcacta gaattgaaag gaaaaataaa atttaaagat ttttatgaat
    14581 caaaatggct tcctagatat gaactaggtc aaactactag gtcaaacagg gctccaagta
    14641 atgtaacaat tagtaatact aaggatatat tccgattgca catccttcca atgtttggtg
    14701 aatatgcaat gaattatctg aattcaaata ctgagataat atcagatgaa ttaacaaaga
    14761 aatcgaaaga atacgcaaat ataaaaatta tcaaaggcta tgttcgctct atgtttgata
    14821 ttgccgaaat actatgttat atcgaattca accgaactac caaaattatt caatgtatta
    14881 ccgcgcctaa gaaaatggct ttagaagaaa aaaggataag ggaagggaat caagcattat
    14941 catcagaaga gctgactgat tggatagaag cagtaaacaa tgacttaaat aatcacttgc
    15001 ttacacttca cgattacaca cttttcatgc ttaccttata tctaggtgat agaaaaagtg
    15061 agacttatgc tctgcaatgg aaatatattg atttt
//
LOCUS       DANKMN010000072        14991 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000072 DANKMN010000000
VERSION     DANKMN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..608
                     /locus_tag="PW224_002255"
     CDS             <1..608
                     /locus_tag="PW224_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908916.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="PW224_002256"
     CDS             628..1656
                     /locus_tag="PW224_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2908917.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="PW224_002257"
     CDS             1653..2411
                     /locus_tag="PW224_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2908918.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="PW224_002258"
     CDS             complement(2497..3237)
                     /locus_tag="PW224_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2908919.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="PW224_002259"
     CDS             3298..4551
                     /locus_tag="PW224_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2908920.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4852..5100
                     /locus_tag="PW224_002260"
                     /pseudo
     CDS             4852..5100
                     /locus_tag="PW224_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="PW224_002261"
     CDS             5222..5548
                     /locus_tag="PW224_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2908921.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="PW224_002262"
     CDS             5616..6329
                     /locus_tag="PW224_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2908922.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /locus_tag="PW224_002263"
                     /pseudo
     CDS             6639..7491
                     /locus_tag="PW224_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7612..9477
                     /locus_tag="PW224_002264"
     CDS             7612..9477
                     /locus_tag="PW224_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2908923.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9525..10934
                     /locus_tag="PW224_002265"
     CDS             9525..10934
                     /locus_tag="PW224_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL2908924.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11042..11743
                     /locus_tag="PW224_002266"
     CDS             11042..11743
                     /locus_tag="PW224_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2908925.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11902..12381
                     /locus_tag="PW224_002267"
                     /pseudo
     CDS             11902..12381
                     /locus_tag="PW224_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13229..13804
                     /locus_tag="PW224_002268"
     CDS             13229..13804
                     /locus_tag="PW224_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL2908926.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13804..14244
                     /locus_tag="PW224_002269"
     CDS             13804..14244
                     /locus_tag="PW224_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2908927.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14260..14961
                     /locus_tag="PW224_002270"
     CDS             14260..14961
                     /locus_tag="PW224_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2908928.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5065 a         2485 c         3161 g         4280 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     9301 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     9601 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     9661 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     9721 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     9781 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     9841 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     9901 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     9961 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    10021 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    10081 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    10141 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    10201 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    10261 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    10321 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    10381 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    10441 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    10501 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    10561 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    10621 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    10681 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    10741 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    10801 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    10861 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    10921 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    10981 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    11041 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    11101 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    11161 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    11221 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    11281 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    11341 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    11401 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    11461 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    11521 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    11581 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    11641 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    11701 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    11761 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    11821 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    11881 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    11941 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    12001 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    12061 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    12121 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    12181 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    12241 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    12301 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    12361 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    12421 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    12481 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    12541 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    12601 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    12661 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    12721 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    12781 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    12841 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    12901 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    12961 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
    13021 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
    13081 aaaatggctg aaaaatgatt tttttctaaa tactatatat agtggcgtct atcctatata
    13141 ccactaaaaa actacaaaat ctagtctttt attttaaaaa aacacgtgta gaatgaagat
    13201 agacttttga gttagggagt gtttttttat gaaacttact gttttttgcg gttcccgttt
    13261 tggtaataaa gaaagttaca aatttattgc gcaaacgtta ggaaaataca tggcacaaga
    13321 agaaatcgaa ctggtttacg gtggttcata ctccgggatt atgggagtga ttagtaaaac
    13381 agtactggaa aataacggaa aagtcacagg tatctatccg aatgggcttt ttgaaactga
    13441 attgcctgga aaagatgtta ctacatttat tcctacagaa acgatcgatg aaagaaaggt
    13501 attattattt gaaaagggag atgctgtcct cgttttccct ggaggtctag gtacattgga
    13561 ggaattctca caacttcttt cttggatagc aatcggtctg acgcccgaca agccaatcgg
    13621 aatattgaat attggcggtt attacagcgg cttgcaacaa ttattagaaa cttttgcaaa
    13681 agaggatttt atggataaaa aatggttgga tcaagtaatc ttttccaaca atccattgga
    13741 actggtcaat gaattacgag cagtatctat taaaaatcag ttattattga aggaggcaaa
    13801 ataatgagtc aaatatattt agcaggacca tttttttcag aggagcaaat cgatcgagta
    13861 agtcgtatcg aaaaagcgtt ggaggaaaac aaaacagtca caagtttcta ctctccacgt
    13921 catcaccaag aaagcaatta tgaactattc agcgctggat gggcgcagga agtatacgaa
    13981 aaagatatgg aagaactgac aaatgcagaa tttgttgttg ctatccttga ctttgagcat
    14041 caaacaatcg atccaggaac ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg
    14101 atgatcattg tacaagaaga gacggtacct acgaacttga tgattacaca aagtctccat
    14161 acgtatttaa aatcagacca agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa
    14221 acttacgtag gcgagtactt atagaagagg aagggttgta tgtcagtgat tttgatttca
    14281 ggaacgattg gtgcaggtaa gagcagtttg acggatatgc ttgcaaaaga gattgattct
    14341 aaaccatttt atgaaaatgt cgaagataac gaagtattgc cgctctttta ttcaaatcct
    14401 gagcagtacg cttttttatt gcaaatcttc tttttgaaca agcgattttt agcaatgaaa
    14461 aatgctttag tgaacgatga caatgtgcta gaccgatcca tctatgaaga tagcctgttg
    14521 ttccatctaa acgctgattt aggtcgggta acggatatcg aagtacagca gtacgatagc
    14581 ttattagata cgatgctaaa cgagttagat gatgttgctc caaaaaaacg gcctgatttg
    14641 atggtccata tcaaagtttc tttggacacg atgctagaac gtatcaaaaa acgtgggcgt
    14701 gactatgaac agctagaaag cgatgagacg ttatatacct attatgaaac gttgaacacc
    14761 cgctataatc aatggtatga agattttgat gtatgtccta aaatacaagt ggatggggat
    14821 aaattcgatt ttgtagaaaa tccagaagat gcaaaaaagg tgatccgtca gatccaagaa
    14881 aaattagctg aattggaagg aaagcctgct ggaaaatatt ttacggataa ccagcatgca
    14941 aaaggtcaag aattaggcta aaaataaaaa accaaacgac gggaaagaaa a
//
LOCUS       DANKMN010000073        14327 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000073 DANKMN010000000
VERSION     DANKMN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            644..1507
                     /locus_tag="PW224_002271"
     CDS             644..1507
                     /locus_tag="PW224_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2908929.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1636..3258)
                     /locus_tag="PW224_002272"
     CDS             complement(1636..3258)
                     /locus_tag="PW224_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2908930.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3507..4028
                     /locus_tag="PW224_002273"
     CDS             3507..4028
                     /locus_tag="PW224_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2908931.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4214..5944)
                     /gene="spxB"
                     /locus_tag="PW224_002274"
     CDS             complement(4214..5944)
                     /gene="spxB"
                     /locus_tag="PW224_002274"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL2908932.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6109..6927
                     /locus_tag="PW224_002275"
     CDS             6109..6927
                     /locus_tag="PW224_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL2908933.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(7030..7227)
                     /locus_tag="PW224_002276"
     CDS             complement(7030..7227)
                     /locus_tag="PW224_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908934.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7396..7974
                     /locus_tag="PW224_002277"
     CDS             7396..7974
                     /locus_tag="PW224_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2908935.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            8069..8206
                     /locus_tag="PW224_002278"
     CDS             8069..8206
                     /locus_tag="PW224_002278"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908936.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(9019..9522)
                     /locus_tag="PW224_002279"
     CDS             complement(9019..9522)
                     /locus_tag="PW224_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2908937.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9881..10906
                     /locus_tag="PW224_002280"
     CDS             9881..10906
                     /locus_tag="PW224_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2908938.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11229..12107
                     /locus_tag="PW224_002281"
     CDS             11229..12107
                     /locus_tag="PW224_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL2908939.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12583..12699
                     /locus_tag="PW224_002282"
     CDS             12583..12699
                     /locus_tag="PW224_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2908940.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13627..13965
                     /locus_tag="PW224_002283"
     CDS             13627..13965
                     /locus_tag="PW224_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908941.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            14059..14289
                     /locus_tag="PW224_002284"
     CDS             14059..14289
                     /locus_tag="PW224_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908942.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4548 a         2406 c         2744 g         4629 t
ORIGIN      
        1 agaataaggg actgtgacaa aaaccgtgtc acagtccctt tttccgaaca aacggtgttc
       61 aaaagcttgc atctacggca ctaagcccaa tcagatgaaa aatatggagt gctattttta
      121 tctgattgtt cttactgctt gatgcagagc agctttttca caacctctcc actaaacggt
      181 gttctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgttt
      241 tttgaaattg ttccttgtga ctcggagcta aacggcaggc tcacaacctc ttggtaaacg
      301 gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
      361 ttttccgttt ttttctctaa tactcaggtc aaaacgctga tttcacaacc tctgattcac
      421 ggtgttccag aagtagcttc tacggaaata agccgaaatt tcacaaaaat ttgagaagca
      481 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctcttattt
      541 tgattagaac ttgcgaaaat aaaccggtta aagtgtgtct agcgcaagag atttgctata
      601 atagacgata agggtaaaat gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac
      661 agattcttgt tgtgatctgc cataccatgt attagcaaaa gaaaaagttg attttttatc
      721 aatgaatctc caattgaatg atcaagagtt agtagatgac ctcggaaaga cattcaatta
      781 tgaccgctac atgaatcagc tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt
      841 tggccgatat ttagaatttt tccgttctta tgcggaaaaa cagattcctg ttttatatgt
      901 ggctttttcc agtggattaa gcggctctta cagtagtgcg cttcaagcgg ttgaattgct
      961 gaaggaagaa tatgaagacc ctgagatcta tgtttttgat acaaaagctg caagtattgg
     1021 ggaagggctt ttagtattgg aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga
     1081 aaccctagag tggctggaaa gtaattattt gaaagtccat tcatgggtaa cagtagacga
     1141 tctgaaatat ctagaacgag gcggtaggat ttccaaagca agtgctactc ttggcagtct
     1201 attgagtgta aagcctatta ttacggtgga taaagaagga aggctgacga atgttgacaa
     1261 agttcgagga agaaacaagt cgatccagac aatcgtcaat gagacggtaa aaacaatcgt
     1321 tgcaccactt gatcaaatcg tttatgtggc ttacgctggg gatctagaga gcgcggagaa
     1381 ggcaaaacgg cttttggaag agcagataaa gatgaaagat gtgaagcttt atcccttagg
     1441 accaacgatc gccagtcaca caggctatgg ctgtatcgcg attttttcga tgggaatcag
     1501 tcgataaaag cagtaactca ttctgtaaaa aagcccagaa attgattgta acaaaaacag
     1561 cgactcttaa aagatagatg agcatcctat ctcttagagg tcgctgtttt tttatttttt
     1621 aagctaaata ggccattatg cccataactc gttcaattgt ttttgaatat ctgcattttc
     1681 caaaaattca tcatatgtcg tatcagctcg atcgatcaca cctttatccg aaacggcaat
     1741 gatgcggttt gccaatgttt ggataaattg atggtcatgt gacgcaaata agattgagcc
     1801 agtaaatgcc atcaagccgt cattcaatgc tgtgattgat tctaagtcta aatggttcgt
     1861 tggatcatct aatacaagta cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg
     1921 gactttctct ccaccagata aaacattcac tggtttcaat acttcttcac cagaaaacaa
     1981 cattcttcct aagaagcttc ttaagaaggt gttatcatct tcttctttac ttgcatattg
     2041 acgcaaccag tctaaaatcg ttagatcagt atcgaattct ttcgttgtat cctttggtaa
     2101 ataagaatta cttgttgtca caccccaacg tactgttcca gtatctggcg taatttcacc
     2161 catgataact ttgaatagcg ttgttgttgt tatatcattt tctgcgacga aagcgacctt
     2221 gtcttctttg ctcaaattaa aagatatatc atctaaaatt ttctttccat caatcgttac
     2281 acttacattt tctacctgca ataagtcgtt tccaatctca cgttcaggtt tgaatccaac
     2341 gaatggataa cgtctagaag aaggttggat atcgtctaaa gtaatttttt caagcatttt
     2401 tttacgtgaa gttgcttgtt ttgattttga tgcattcgca ctaaagcggg caataaattc
     2461 ttggagttct ttgatttgtt cttctttttt ggcatttgcg tttgcttgaa gtttagccgc
     2521 tagttgacta gattccaacc aaaaatcata atttcctaca taaagtttga ttttaccaaa
     2581 atctagatcc gccatatgtg tacatacttt gttcaaaaag tgacggtcat gggatacaac
     2641 gatcacagta ttttcaaaat tgatcaaaaa ttcttccagc caattgatcg attggatatc
     2701 cagaccgttt gttggttcgt ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc
     2761 taataacact tttacctttt gtccaccagt taattcactc atttgctgat ggtgcaattc
     2821 ttcagggatg ttcagacctt gcagtaaaac agctgcttct ggttcagctt cccatccatt
     2881 aagctcagca aattctcctt ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc
     2941 ctctttcatg taaatcgcat ctttttcttt cagcacttca tacagacgct tgtgtcccat
     3001 gatgactgtt tcaagtacag tatagtcttc atagtcgtaa tggttctgtt tcaatacggc
     3061 catccgttca tctggtccca tagtaacgga acctgtggaa ggctgcagat cccctgataa
     3121 cactttaagg aacgttgatt tacctgcacc atttgcacca atcaagccat agcagtttcc
     3181 gggggtaaat ttaatattca catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac
     3241 atcatttaca gtaatcaatt tttttcctca cttttatcat tattagtttt gacaagtctt
     3301 atagaattat ataggggaaa ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca
     3361 cgtgaaacag aatgaaaaaa ataaaaataa aagcggagcg aaaatagcta ctataggatt
     3421 tattagtttg caacagcata atttttgacg ttatttataa gagggagtac tataaaactg
     3481 taaaagaaat gaaagaagga ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat
     3541 tgattttggg aattttattt gtacttgtct cattgttatc atttcaagac cctgtcggaa
     3601 atttagttgc aattgtagta gtctttgcga tttttgcctt tattaaaggg atatttgaac
     3661 tgtttgtacg gaacagatta aaagagctaa caggatataa aggaaagatg cccttaataa
     3721 tcgggatcat cgatattcta gttggcgtct ttttcttatt caatattggt gcaggagtcg
     3781 cagcattgcc ttttgttttt gcagtatggt ttattgctga ttcagtttta gcgttattta
     3841 ctgctgactt ggcaagagga gtaagtgaag gatattattg gtttacgatc atcgtcaata
     3901 ttttaggaat tttattagga attttcttat tgtttaatcc aatttcttct gcgttgacat
     3961 taagcttttt agttggattc tattttatgt tatttggtat cacacatatc gtctatgcat
     4021 ttagataagt aaaatcaaac aaataaaaaa gccggcttcc gattacagtt ttactgttgt
     4081 aattggaagc cggctttttt atcatggaca aatatgcacg aggtgatttt taggtgtcga
     4141 gacaaattta tgcaaaaatg tgagaagcta tatacacaaa ttttatctta atactcgaag
     4201 catagcgcag ttgtcacaac ctcttttcat ataattcatt caatactggc aaatcatgga
     4261 cttcatattt ttctttgaat gcttggattt cttcttcaga atattttctt ggatctaaat
     4321 gcagatcttc tactggcaat gggcgcttgt tattgatctt cacatcgatt actacaggac
     4381 gatctgattt tgcagctgca tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa
     4441 agctatttgc cccgagcgct tctccaactt taccgaaatc tgcatcttct aaaaatacac
     4501 caaatttctt ttgttccgta tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg
     4561 aaaagacaac attaataatt ggaagctgat atttgacttg tgtaatgata tcttgcatga
     4621 ccatagcaaa cccgccatca ccacttaagg taaatacttg tttttctggg tagcttagtt
     4681 gtgcggcaat tcctcctggc acaccattac ccatagttgc gaaccatcca gatgtcgtat
     4741 gtttttgttt accgttcata ttcaataaac gaatagaatg gatagtcgtg ttaccgacat
     4801 ctgtgacgaa aatcgcatcg tcttcagcaa tacgattgat ttctttatag acagcctctg
     4861 gacgaatcgg ttcttcctct cgatcttcaa aagatttcaa ccaatttacc catttttctt
     4921 tattttcttg atttgctagg taccagctat cagcagggcg agcatcccct aactcgacaa
     4981 gtctttgcaa agctgtgttt gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc
     5041 caaattttga cgcatcgata tcgatttgga taaattcagc agttggatta aaaaatgcac
     5101 gtccaaatgg gaagtcgcta ccgacaaata agatcagatc ggcttctgcc aatgcttcat
     5161 ttccgggttt agtagcgaca cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa
     5221 taattccttt tgctagaacg gaagcgacaa caggaactga aaaatgttct gagaattctt
     5281 tgatttgcgg aaatccgttt cgcgttcctt gaccaacata aagaactggt ttttttgctt
     5341 tttcaagata aggcaaagca gctagcaaat ctttttcttc tggtaatatc acacttgttt
     5401 tgtgagtgtg agcggtggag aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag
     5461 ggatagtcac gatagccacg cctttatgct cataggcggc tttaatggct tcatctacta
     5521 catgcggaag actttgtggt gtcatgactg tacgattgta tacgctaaca tctgcgaaaa
     5581 taggattttc atttagttcc tggaaagagt tatagttcat ggatgtagaa gcaacttgtc
     5641 ctaagaacgc tactagaggc acatggtcca tctgtgcatc atacaaacca ttgatcaaat
     5701 gtgtcgcacc aggtcctgca gagccgaaga cagcgccgac tttccccgtt aattttgcat
     5761 ctgcagcggc tgcgagtgcg ccgacttctt catgacgaac ttgaatgtag ttgatttcgg
     5821 ttttttcttg atatagcgca tccataattg aattgaaaga tcctccggga atgccgtaaa
     5881 tatggtcgat accccagctt tcaagaactt ttaccattgc gattcctgca ttgatttttg
     5941 acatggtgtt tcactccttt agcaatgatg atacataaat tttttgtatc tagagtatag
     6001 aactttttat cgaaaatccc aactaattcg tcttttcaca aaaatgtagt catgtttttg
     6061 ggaatatgct agagtagtac atagatagat tcaagagagg ggaatggcat ggttgaatgg
     6121 ctattgagac ttgaaccttg gcagcaagca cttgctggga cagcttttac ttattttatg
     6181 acagctcttg gtgccgggtt ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac
     6241 ttgatgttag gatttgcttc tggtgtcatg attgctgcaa gtttttggtc tttgttagat
     6301 ccggctatta caaaagcaga agaaaatgga gatattgctt ggctagttgt aagtattggg
     6361 tttggtttag gcggtgtgtt tttatatatg gcagataaaa cactgcccca tatgcatttt
     6421 ggaccacaac atgaagcaga aggattgcct acccatttaa aacggacgat cttgcttgtt
     6481 ttttctatta cattgcataa cattcctgaa ggtttagccg tgggggtagc attcggtgca
     6541 gcagcaactg cggataatcc gacagccgct atattagccg ctgtttcggt ggcgctaggt
     6601 attggtatcc aaaacttccc agaaggagca gctgtatcaa tcccgttgcg acaagaaggt
     6661 ctgagtcgta aaaaggcctt tgtttacgga caagcttctg gaatcgttga accaattgct
     6721 ggagtgattg gagcgctact agtaacgaaa gtggagctgc tgcttcctta tgcacttgct
     6781 tttgccgcag gagcaatgat ttatgtcgtg gtggaagaat tgatccctga agcacagcag
     6841 acattgtcca gcaaacatca ttttgcagtt tttggcgtca tgagtggatt tatcattatg
     6901 atgattttgg atgtggcatt aggataaata aaaaaaatat atatgatata tcaaataggg
     6961 ccgcgaactt atatcgcgac cctatttttt tagtaagtta tatcataatg aagtgaatgg
     7021 tatcatacat taataatagt tttttctatg tgtatttcct ttgatccagc gaacaagcca
     7081 gatgataagt gcgattggga ttagaacttt gaccgcaaac cataaaagcc cgcttaggac
     7141 actgacaaaa agactgatga cgaagctagc tgcaattagc actaaaagaa ccatgaagat
     7201 atttaagaac atattttttt gacgcataca aaaccctcct cttgttcatt ctttactata
     7261 gaaaaagtat aacagggatt gtttaaaaac acctccaact gaagactgat ttttacttca
     7321 tctacttaaa tgatcaaaca attgcttttt ctctggttta tcatctaaaa tagagcggaa
     7381 aaggaggaga atatcatgcc taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg
     7441 attatcatga ctattttagt tttagaactt catgaaccta acggagacac gtgggaggcg
     7501 ctcgctgcat taggttaccg attttttatc tatattgtca gttttatcat tttagcgatt
     7561 tattggaaca atcatcatca tttatttcaa gttgtacata agataaatgg acgggtctta
     7621 tgggcgaaca gtttctttgt ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc
     7681 agtgagtaca tcaatagcct ggctccagaa atgacttatg gttttgttac cttgaccgct
     7741 aatattgcgt attatatctt ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa
     7801 gcattgaagg cgatgttgaa taattacaag aaatcgtatc tatcgattgg tttgaatgtg
     7861 ttaggaattg tcttagggta tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg
     7921 attctagctg cttggatcat cccaagtcgt accatcgaat cacaatatcg gtaaaatgat
     7981 ccgtgtttaa aatttaagag aaaaatatag aaaaaataaa taaacgatca aatatccgag
     8041 tgatactagt attcaaagag ggatgcagat ggatcaaaaa agaattcatg taggactgat
     8101 tactagtttt gtcacgatgc ttatttattt ggatcaaggt catttgactt ggacagtagt
     8161 tggtgtcgta ggatttttga cattattcac agcaataaaa aaataaacat ctttatgtaa
     8221 tctgatgttc tatctattta agaaaagcat gtttacagca gtatgattct gttgtaaacg
     8281 tgtttttatt tgtggaaaat ttaattttta gaaaagtgta tcgaaaaata gatagttagt
     8341 ttttccacag tggataactt cgtaagagat aaaaataaaa aattagtcaa agaagggcgt
     8401 atagatttct tttgatagga gatatacaac agaaatgaat aaatattaac gtgttttcaa
     8461 acagtataaa acggtgtggt ttttggaata aaaaaataac atatctcatt ttacactttt
     8521 gttattttat acttaaaaaa taacgacaca gaaaataccg tgtctcagat ttctctacta
     8581 cttctaaaaa ctactgtttt tttcctttga aatgccttca aaaagtagga tcaggcagaa
     8641 tatactggca gaatcaagga gaagggagcc taaataagga gaaaaaccat agaaaatata
     8701 aagtaattac ttataaaaca agctttttat agaaaaataa tatacctgag tagggtataa
     8761 attaagacgg aattcctttc tagattgaga tattgactag gaagaatgtt aagattttaa
     8821 gcctttaagc taaaaaaaaa ccctttttgt taaataaggg gaatatagcc tgtcagtttc
     8881 aaactgcaag tcgtcctgct gcaataatat agtagattag agatgaaaat agattttttt
     8941 aaaccaatga ctgaataaaa agaccgtcat agatacacat tggaatgtgt ctattacggt
     9001 ctttttttac gaacgatttt attgaagacg gacagttcgg gtcaaaaata aaaaagctac
     9061 gatcagaaat aagaataaaa tgtatcccaa aataatggct agacggtcta acttatttgt
     9121 cgatagatgt ttgtttagaa gttgataggc ccataaacct atcagtcctc ctgttgtatt
     9181 agttatcaaa tcagtgatgt ccgctacgcc aataccgaat aggaattgaa tcaattctgc
     9241 caagaagctt acaaggaaaa taatccctaa ttttttagaa aaagaaagtc tcttgaaatt
     9301 gacattcaaa agcaccccaa aaggaaaaaa gacaatcaag ttataagtgg tctcacgtaa
     9361 taccgtcgta ttttcaaaag aaaaagtaga aaaagggacg agattgacat tgctgtaaga
     9421 ataagtgaga aattgtggaa gattaaatga tagcttgaat aaaatcaacc ataatagaaa
     9481 gaataaataa atccctaata aaaattttga tacataccgc atagacatct cctccattta
     9541 aaaagtatac catcgaaaac cgctgaaaaa tcataaaaaa gtatgaagtt taaggaccaa
     9601 cgtagttatc gtacgaaaat tagaagcttt tttttgttgg gaatccggtt tctttaacaa
     9661 agctaagata gcgagttgct tggaaaagtt gctatctttt ttctgtcaaa acgactcgga
     9721 atgttcgttt tttgttctaa tttctccaag ttgtttactt tttgatacta agtttatgaa
     9781 aaatcaagaa atagttgtta tactaggtaa tgctgacaaa cgggattggc atttcatctt
     9841 ggttcagctt tttttccaat tactaggaag gagggataga ttgttcaaga cttattttcg
     9901 tttcaataaa atacaagata atccttttcg tatcatgaat ctgatgctac ttatctttat
     9961 tgtattatgg ttaggttaca taaatcatga ttttttgatt tcaagttttt catcattagg
    10021 gatctttact tttttctatt atcaaaacat accgatgaaa tatttgctga aacgtatgtt
    10081 tctgatcggt tgcggactgt tggttgcttt cgttttgggg atcttatcga cttatgtttt
    10141 atggcttgaa ccatttgccg tagctttggt tgctttttcc agcagattcg tgttgcgatt
    10201 attccatatt tctaaaccag gtggcttgtt ttttgcgatg ctttctgcca tgggaacgag
    10261 catgcagctg ccaatcgctc aattaccgat cgtctcactt tattttttta tgggtgtcgt
    10321 ttttgccctg attgcggcag tgattacaaa attactggat tcacggcctg agcagacaat
    10381 tgaaaaagca actttaaaag aaagatttca cgaagagcct ttagtgatca ttgattctgt
    10441 tttctacagc gctgccttat ttctttccgt ttatgtcagt cacggcttga atctgcataa
    10501 tccttattgg ttgaccttgt catgtgcatc gattttgcta gcagaaaact tggatgcgat
    10561 gaagcatcga caagtccaat atctgattgg atcgatgggt gggttatgtg tgtcggcatt
    10621 cttgtcattt gtgccgttta cgcagttgca gaccattttt ctgatcactt ttctatatgg
    10681 aatcgcacaa tttctagttg ctagaaatta tgcagttgct aatatttttc ttaatccgat
    10741 ggcgttgatg ctatctacac ttattcgtgg agcatacttg atttctttga tcgaatatcg
    10801 ttttttaggg atcgtcatag gaagtttcat cggtttaggt gttgcgtggg taatgacggt
    10861 tggtttgcaa cattatttaa cggttgttcg tcaaaaatta gaatagaagg acaaatatat
    10921 gtgccaaaag aggttatgac aagatttttg tcacaacctc taattcaagg tgttcaaaag
    10981 ccagattctc agcattaagt caaattcaca caaaaatgtg gaaaggaact ttcaaaaaaa
    11041 ttttatattt attctatcaa aaaattttgt ttatgatggt aactgaataa actttcctga
    11101 gatggtcaat gctttccttg aaagtcttac agatattctt tcttcataga aagtttaagt
    11161 tttatagtta gtgtacaata aaagtatgtt tataccatat ggtgtccgga taatcagtca
    11221 tttaaacaat gaggttcaaa gatatgaaaa ggggaaattt gatgaagaaa ataagcaatg
    11281 aaatccaaat tctggcagaa actttaactc ttcccaaagt cgttatagag caaatgattg
    11341 tacaagcaaa aaacatcctt gatgaggtgt ttgagcaata tagtcatgaa ctaatggatc
    11401 ctaataaagc aaaggcgact gcaaagaaaa tagacaaagt ctatgaagag gaaccttttg
    11461 cagcattagc tatttatcta tatgcagctt ctcaaagttg gaaaaaaatt tatcaaccat
    11521 tgaagattcc aagaactatc tatttggcaa caatgaatgc ttttactaga tttattcatg
    11581 aacatttcca agtaacagga agctatcgct ttgaccgtgg tttttggatt tggcattaca
    11641 ctagtggctt gatctttcgg attggtgaat tggagtacga aatgacatat tttccgaaag
    11701 ggtacacgga tctaagatta gaaggaaagc agtgcctttc tcttcatatt ccttccgatg
    11761 cagatttatc tcagaataag ataagagata gttacgaaag tgccattcgt ttttttgaac
    11821 tttattttcc tgattaccat taccaagtga tgtacacaga tacttggtta ctatcaccca
    11881 atcttactaa atggttgaaa aaagagtcaa aaatctgtct ttttgcagcc gattatcgat
    11941 tattatcagt tgatgaacag gacgatagtg gagtcccttg gatctttgga cgagtagatg
    12001 cccaaataca tgattatcca gaaagtacga gtttacagcg acaagcaaaa gagcaattgc
    12061 ttgcaggcga gcacattggc tctggcttag gtattcttac ttcataagtt tagtacttcg
    12121 aaatagttta aaagagcgtg agacaaaagt aaagaacact tttgtctcac gctctaaaca
    12181 cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
    12241 tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc
    12301 ctctttttta gtgttctatc gaacacacag tacgttcaat aggtaaacta gctataacaa
    12361 tagatattaa agttaacgga caagagggcc ttttttaggt agctaaaacg taataatgaa
    12421 aatttatttc cagagatagg agtcaatcag ctagtctgat cgactgtttt aacaagttga
    12481 ctttcatttt aacttttgat accatttaaa acacaaatcg taattgtttc gatttgtgtt
    12541 ttttagcatg actacaatca aagaagaaag tgggtcgtcc atatggaaaa aacagatatc
    12601 tcaagtgcat accgccgctt gaaaagccca aatatcaaaa cacgaaaacg tgcgctgaag
    12661 atcatcaaag atgtgaaacg gaatagtggg aaaaggtaag aagaaggaaa cttaattaaa
    12721 aaaatagccg aaaaacgttt ttcggctatt ttcaataatc ggtttttgtg taaggtttcc
    12781 gaaagaactg ctgttgattt tccattttta acatatgata aattggtaga atctttcttt
    12841 tcaaattgat accattccct aattccagtt atatagttaa ctacagaacc acaaagcacg
    12901 tacgtttata aaagtattat ctgtagttat ttttcaattc cctttcaacc tcttattttg
    12961 accagtagta ttgatcactt taccttttaa cacctttgtt cgtttttgaa acatttttag
    13021 tcaagaatgg aaacgtaact acataatcaa aggttttgcg aaatatttca tgaaatattt
    13081 catgtagaag cgagcaatac ggttgcaaat aaccattatc gtttaataag cagttacgaa
    13141 agctcccaat atctctaacg ttcagctcac tccatttctt ttcttcaaaa aaatcagtga
    13201 ttcgtatctc tttctatttg tcgaaaaagt taactttaaa aagtaacgcc gaaaaaatat
    13261 gcaaaaaaat aaaaaagcag ttactattgc tagaataaca tcaaggaata ataagttttg
    13321 gttttcagat aatagtcgat aagttgtgta ttggaatttt agatacagta ataaagtatt
    13381 tttcatgaaa ttttgtgaga atcactatta caagtatctt taataaaatt aatggttaag
    13441 gaggaactta aagtagaatg taaatgaata gttgtagtca gtcgattgtg atactagact
    13501 caaagttgca ggaataagtt tttcaatttg tgattggaag ataaaaaatg aaaaagaagg
    13561 gctttttttt atactttgtt gattgtagtg tttattttcc ctctgtttag ccaagtaaca
    13621 ttttctatga ctcaaccagt ttcaagtgag gaacatgata acctttttcg tgctacagaa
    13681 gagtttaaga aatatcagga aaatattgtt ccggagactc cattagctag tgagccaatt
    13741 actgatattg atggaaacca aataggatgg tatatacagt acgaagttat ggtggtaaat
    13801 aatgactcaa tagtaaaaaa tacagtaggc ttagagctaa caccgattga aaaggtgagt
    13861 tcattgttaa cgtatattta tactgatgaa agtgacgaga ctgagatcat tttaacggat
    13921 tactcaaaaa ttgctactga taaaaagtat atattgtgga tgtagacact agtgaaatac
    13981 tttatgatat agaaattgat gaaaatttac tagatgtagc acaagaaatc gatcatcaaa
    14041 agcaggaagc tattcgatat gctggtagaa aagggaaaag aaaatattta ttacgtgaat
    14101 gtagcaaaag ttagagaaga tgaaaatgag tggaaagaat tcaagagtcg ctactcaatt
    14161 aactcgactc cgacatttac agtttacaga gaaggatcga tagaaaaaac tgttttttgg
    14221 actaaagaaa gtggaatgtc tttagctgaa gtagaagagt ttttggatta tgttagtatg
    14281 caacagtaag ggagttctta ttatgaaaaa ttttgacaca tattatg
//
LOCUS       DANKMN010000074        14208 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000074 DANKMN010000000
VERSION     DANKMN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(45..476)
                     /locus_tag="PW224_002285"
     CDS             complement(45..476)
                     /locus_tag="PW224_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDL2908943.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(827..1519)
                     /locus_tag="PW224_002286"
     CDS             complement(827..1519)
                     /locus_tag="PW224_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2908944.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1956..2525)
                     /locus_tag="PW224_002287"
     CDS             complement(1956..2525)
                     /locus_tag="PW224_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2908945.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2599..2922)
                     /locus_tag="PW224_002288"
     CDS             complement(2599..2922)
                     /locus_tag="PW224_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908946.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3076..3888)
                     /locus_tag="PW224_002289"
     CDS             complement(3076..3888)
                     /locus_tag="PW224_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908947.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4242..5090
                     /locus_tag="PW224_002290"
     CDS             4242..5090
                     /locus_tag="PW224_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL2908948.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5235..6176
                     /locus_tag="PW224_002291"
     CDS             5235..6176
                     /locus_tag="PW224_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL2908949.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6166..8337
                     /locus_tag="PW224_002292"
                     /pseudo
     CDS             6166..8337
                     /locus_tag="PW224_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(8449..10620)
                     /gene="copB"
                     /locus_tag="PW224_002293"
     CDS             complement(8449..10620)
                     /gene="copB"
                     /locus_tag="PW224_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL2908950.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10640..12826)
                     /locus_tag="PW224_002294"
     CDS             complement(10640..12826)
                     /locus_tag="PW224_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL2908951.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12826..13035)
                     /gene="copZ"
                     /locus_tag="PW224_002295"
     CDS             complement(12826..13035)
                     /gene="copZ"
                     /locus_tag="PW224_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL2908952.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13048..13488)
                     /locus_tag="PW224_002296"
     CDS             complement(13048..13488)
                     /locus_tag="PW224_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL2908953.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(13562..14101)
                     /locus_tag="PW224_002297"
     CDS             complement(13562..14101)
                     /locus_tag="PW224_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2908954.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         4041 a         2919 c         2483 g         4765 t
ORIGIN      
        1 gtacaattag tattatctgc actactttct aattaaataa atttttagtt attaacttga
       61 ttaaaatcag agataatatc atttaattca gtttgaaggg ttgtaacgat taaatggaaa
      121 ttttcttcat ctgttttatt atatggggat gttttcgtta tatataacaa cttttctttt
      181 tcaattttca ttaataactc tgtcgaagtc tgtactttat cttgataatt aaatgcagac
      241 ataccacttt gtataattgt aattattgct gaagcaattg aaacaatcaa tagtaaagac
      301 ctgtgttcgg aagcctgatc aattaatatt gggatactag ctgataagac tatttttata
      361 atatttccga taccaattac tctattataa aatcgaattt ctttttttaa tctatcgatt
      421 gtagaatcaa tagattcgat aaacgctttt tgatccatca ttttctctcc aatcatttta
      481 ttaaaatcat taacaaaaat attataatag gatttaagta agttctaact aatcacatat
      541 aaaaataaaa aaacatttgt attttatcaa agttttatta tacttgtcaa tttgtgtaca
      601 tcctaaaata taagctattt gtttacttct gtagaatata tactttgttg ttagataagt
      661 tgcacatgtt tatagaatct aaataataaa tttttatgta ccgcccctca aagtgaactg
      721 aaccccaaaa gttgaactat ttaatggact gtttccgata ttctactgga gatagtccat
      781 taaatagttc aacttttggg gttcagttca aagagggggg gattttttat cgttgtggaa
      841 tatttaaata ccaacgcttg tcatgaaaat cttgtgctcc acccttagta tttccttctg
      901 gatcgtttgt cgcacgcatc atgacgtata ctttcttatt agggaaatca cgcatattga
      961 aagatacatg atagccaaca tttcctaaag tattataagc ttgatttacg tctggtctat
     1021 aaatcccatc agctcttact ctagctagtt cttttccagt attgtagtcc ataatgaaaa
     1081 tatactcgta tttataatta gcaatgtgcc atccagctac atgcaagttt gcgttttcga
     1141 tttctccgaa ctgatcaatg tgggcgtaat ttgttccatc tgtcagcgta gaatttgcag
     1201 cacctgctcg agttggatca attactggtt tatcctccga agtagttgga ttatcatccg
     1261 taaaaccatg agccaaatca taggctagtt tttctttact tacacccatt tcagaaagat
     1321 aaccgtaagg atctgtatga tcgccccaaa tattttgtgt tacccataaa tgcgatttga
     1381 ttcctggttg gttataagga gtgtctaatg ttaatggaat accatatttc attgctgaat
     1441 ctcttgccaa ttcaacgtat actttatagt tcttttcaaa agtttcttta tcatgtgtat
     1501 gttgtaactc gatttgcaca ggacttttgg ctcacgaggt tgtcctactc tacatcttgc
     1561 tgatagatat gctgaaagga aaacaccaac attatgtttt gctttttcag ctagaacgag
     1621 acaagcagga atacctttag aagatatcaa aggtttttta ggtcataaag atgtttccac
     1681 tacacaagtc tatgcccata tttctcccga agtgaaaaaa agatcaatga atcaactaga
     1741 aaactatata gaagagcaaa taaaaaagca ctcaaattga gtgctttttt aagctcacta
     1801 tcactaaaac tatcactaat tttatttata agcgaacatg tttctatcac tgatttttta
     1861 aatctagcga aaaaccctta tcttaaaggc gttcgttcaa ttctttggca aggtcttcaa
     1921 agcctggctt gcctagaagt gcgaacatgg ttttcttata gctcatgtag aaaaattttc
     1981 ttgatgatga ttttatccat caattgagat tgactgtttt gctctcctat gatttcagct
     2041 atgattgtcc gattttgcgg ttttcttctt aactggtagt tgattttccc agtttccatc
     2101 aactcgctaa gcatatccac gactccgcca aatccggtct ttttcgtaat aaaacctagt
     2161 gctaccattc gttcatctag attttttgct gtaatcgtca caccagtaat gagcgagtgc
     2221 tgtaatgttt gggccagctc ttccatccct tcagcattgg atacgacgga agctgttttg
     2281 ttaataggtt cttcgtttat ttcttttggt tctttcctct ccactttcac tgtttgacga
     2341 aggacatcta gttcttgcaa gagcgtttcg tattgttctt gggtcagcat cacgccagct
     2401 actttttcac gattaaatat atatacgcca gttcccgcgt ctcgcgcttg agcaaataca
     2461 tccatcggcg atcgtttcac ttctgtgatc gaagatgtgg gaacttctaa tttctttaat
     2521 tccaatggat ttcctccttt actaatcgct ttttattata gcacagactg acttgttttg
     2581 atcttttttc caaaggtttt atcggttttt gcctaaccag taattgatat cggtaagaag
     2641 tctttcgata ccaaatgaag gatggtacag atacgttttt tgatcttcgt tatggtctag
     2701 tggaaaaaaa tgatcgatga tcactagatc tgcataccga ggcggcttat ttcgagtaca
     2761 tacatgtata gagatccgtt ttcgatgtct tagcgttttg ttcagtaccc cagcaagtct
     2821 tcttgcttct tggtgttcat agcgaaaaaa aaagacgatt ttcacttcag gagggtggac
     2881 atctaaatac cctcgttcct ggagtgtttt cagtaaaagc atcccccact ctactgcggg
     2941 gtgtgcaagt gcttgttgga tcgctagccg aaattgatat agtttatctt tggaattggg
     3001 aaatttttct aatgtttcct gataataacg aacttcttct atcagccggt catggatacc
     3061 aattacgtaa cgaagtcatt cttggtaaag tacttttgtc agctttgatt tgctttcgct
     3121 ctcaaattcg ctattcagcc aaaacagcga atcaagcttt tcagtcacaa tcgtcaattt
     3181 ccgatagtct tgtgtctctt ctatccgttt ccaccgcttt tcagtaatct ggttacagct
     3241 caagcgcaaa attgctagat aatttttttc ttttattggc aaaatctgct cctctctaaa
     3301 tagcaattca ggaaacaatt caaagaatag tgttcgactg tctcctaaaa tctcgatttc
     3361 tgccggatcc tttctttgct tcaataattg gatgaacatc tcagaaaagg actgctctcc
     3421 atcatctgca aaaaaatgcg gaaaatcttt caaaaagcaa aacagcagat atcttacttt
     3481 tctttctgaa ccaaagatct gtgctttttt ataagaaaaa gaaaggtggt actcgccaaa
     3541 ccactttctc aatcgctctt gcgtagaacg atcgtacttc tgctcgtaag gttcattcaa
     3601 tagcagattt ttcagtaaac aaaacggggg tgattggatc agaaaaacaa acaagagata
     3661 aggttcgcta tatccttgat cataaactaa ctgaacggtt ttcttatgaa gaatcagctg
     3721 aatgggacag tcgctttccg ccaaacgttt cagatgccga tccactgttt taacatcgat
     3781 tttcagttct tcagctaaat cagcttttcg ccagatcaaa tctttcatta gattgattag
     3841 tgaaaataaa ttctgtgcat tttttgataa tagtttttgc catctcatcg atcatttctc
     3901 acctgatttc taaacatatt gtttgatcaa aatgggttca ctcctaaaaa gataaaaaac
     3961 tccctttcta tttatttaaa tacgcaaatc ttcctcatta ttttttattt tactcaattt
     4021 aatcgtattc tcatttcaaa acgaatgtac tttaaaaaaa cataataaga tcactttgct
     4081 tgtaggatgt attcccttcc attacaatga aataaatagc gattttatga gaaggtgatg
     4141 tgaaaatgga agaaaaaata gaagaatcca aacaaaaaat cgatcagttg atataagaat
     4201 tagaaaaagc ggtcatcggc aaacgttcaa ctatccaatt gatgatcgtg gccctattag
     4261 caggtggtca tgtcttattc gaagatattc ccggtgtagg taaaacgtta ctggtcaaag
     4321 cactagcaaa agctgtacag ggaaccttca gcagagtcca atgtactcct gatctgctgc
     4381 ctagtgatat tcttggtttt tccgtataca attcacaatc aaaagagttt gaatttcgtc
     4441 cgggaccgat ctttaccacg atcttgttag cagatgagat caatcgtacg actcctcgta
     4501 ctcagtcagc acttttagaa gcaatggccg agaatcatgc aacgatcgac aacaatacat
     4561 accctctaga caatcatttt tttgtcttag ctacgcaaaa tccaatcgag tacgaaggaa
     4621 catatccttt gccggaagca cagctcgatc gttttttatt ccgactacaa atcggttatc
     4681 cttcttttga tgacgaactt ttgctacttt tggataaatt cgaaaaagtg ttggataact
     4741 taaatgaagt attgaacagt tatgaaatag aagaaataaa aacaaatgtg gcgaatgtct
     4801 ttgtcagtcc cgaagtggca tcttatgcct tgcagttagt gtcagcaaca agaagtcatg
     4861 ctgccatcca gcttggcatc agtccacgag gaagcctttc ctttattcag gcagcaaaag
     4921 cttatgcttt aatccatggg agaaattacg tcacgcccaa agatttgcag gatttagttc
     4981 cttacgtgtt ttcccatcgc ctgatttttt acgatcgttc attgaaggaa gacgaaaaga
     5041 aacaaatcat tcatactctt gttgcacaaa ttcctattcc tgtgaggtag attgaggtag
     5101 acaatggaaa aaatcaaaga ttatttcatt aattttgtgt tatttctggt atatttcatc
     5161 attgtgcttt ttgctcttgt attcaacaat tctttaggct ggttcttgtt ggcctttttt
     5221 actctttatg tatcatggag cttgatttca ttaagcagct ttctaaagaa actttcggtt
     5281 acttgtacat tgcagccact cgtttatcgc aaaaaaagta tgcaggttat tttttccata
     5341 gcaaaaaaga gaactttttc tttacctctc cctcgtttac aaatcggctt cccagcaatc
     5401 tttgcagaaa aaaacaagag attttgattc ttacaaataa accgcaagaa gtccaattgc
     5461 tttggcagcc aaaagaaaga ggatgccatg aaacattaac tgtcattttt acagcctatg
     5521 atagtctagg acttttcaga aaaagtgcaa aaagagagat ctcttttcct gtgactgttc
     5581 tgcctgcttt ttgtaagatt caagcagaaa aattacagag agttctggaa aaaaaacagc
     5641 agctgaacgc gtaccaaaga ggtcttgatt ttcgggaaca tcgcgcttat cgtccaggtg
     5701 attctttcaa ccgaatcgat tggaaattgt ctgctcatac tcaagagtgg ttataccgtg
     5761 aatacgatta tcaagaagaa gaatgttctc ctcttttttg cttttggggt tcgcccagtc
     5821 caaaatttga acctctgcta gacttgtatt tttctttatg gcatttgaga aaagaaaagc
     5881 agccggaact tctgatatta ggcaaccgcg cctttcatgg caaagacccc ggtcatacct
     5941 attttgcttc tttggaagct ggcgatgaga aagctttttt cgcacacttg aaaaaatatg
     6001 caaaaaaaca aatcgtcctc tttgttcctg agaattctcc tgaagtctca gaagctattg
     6061 aaacattgtc aaaagatcga acggtttatc ttgtttattt cgtagaaaaa caactcatgg
     6121 tccaagaaaa agacaagata tgttcattgc caggaggtga atggattgac gattaaacgg
     6181 gaaaaatggc tgcgtggcgt ttactccttt gtgatactaa gtgtaggtgc tccgatattt
     6241 gtagtagcta atcaattgca aaacagctac cttcttatcc taactattgg tgtcctatgc
     6301 cttattgtct gctgtattcc taaaaaatgg gtacgtttgc tgctttcttt gccagtaatg
     6361 ataggttgtt tgtttttata ttttccttcg aagacatttt ccatattatg gatcttacag
     6421 tttttttcgt cagtcacaga ggaattcttc catctatccc aaggtaaact ttattatttg
     6481 ccagaaaaga cagctttttt tctcataatg ttgctgatct atttatttat tacattgacc
     6541 gtggattacg actattggta cgcacccttt ctttctttga tggcttactt catgatgctt
     6601 accgtttttc ggaaaaaaga tttactgtac gaaatgattg cggtagtaac tgttttgttc
     6661 gtgtatctgg cagcaaggca atttttctcc atccggttgt tgtcgggaaa cactcgattc
     6721 cagtcattga ctacagttct gcttatttgt ttcttaactt ttagcgctat cttgccttta
     6781 tatcttccga aagtacacca atctctcgag gaaacgagtg aaccgatccg agaatatctt
     6841 aatgatgaag ggctttatga cacactccat gagtatcaat taaccggaag tgcaagaaca
     6901 ggcttcagtg agaatgatga acagcttggt ggacctttat atgataatgg cgaagttgtt
     6961 tttacagcga accaaaaagg aaatggctat tggaagatcg aaaataaaaa tcattacacc
     7021 ggttctggat ggatagaaga tcaggacgca cccatccaac caatccaaga attccctgtt
     7081 gacatcacga gctaccaaac cgttatagaa gacaaagaaa acattacatt gaccctaaaa
     7141 aaacaagttt tcttacctta tccttatgga aatactacct tgactgattt ttcattagct
     7201 agtggacaaa cgctttacta tttgcctgaa agccagcgat tcattcctgt cccgaatgaa
     7261 tcgacacgtt ctgcaacttt acaaacagaa aggttagttt ttacgagaga agaattaaaa
     7321 agcagtcagt taaaagctga tgatttagaa aatcagcaga caaatcttca attgccaaaa
     7381 agtttgccta aacgtattga ggacttggcg gtcgaattga ccaaaaatga agcaacttta
     7441 attcgtaaag tcgaagctgt ctaacagtat ttaaaaacaa gcggaaattt ccgctattcc
     7501 aaaacggata ccgggcatac tccaaaagaa cacgattatg tggattattt cctctttgat
     7561 tcaaaagtcg gctattgtga caacttttca acttctatgg tcgtccttct tcgaacactt
     7621 ggtatcccag ctcgctggac aaaaggtttt acaaatgggc tcagaacagg cacagataat
     7681 caagggaatg aggaatacag cattctcaat agtcatgctc attcttggcc agaggtatac
     7741 tttcctggat ttggctggct tccatttgaa ccaacccctt catttgccaa tccaatgggt
     7801 tcacaaccag ttgctgaaac aaccgaaacc gtccagcctg ctatcgaaca accaaatgaa
     7861 agcgaaaata tggcagaaac tatttccagt gagagatctg aggtcattcc atcagaagaa
     7921 acaacactca caccagctta taaaatcgtc ttattcagcc ttggagtgtg tttatccgtc
     7981 ttgttgttct ttctattgtt attcagaaaa tctctgcgct ggacaagtta tcttatctat
     8041 cttcattttt ttgcttctac taatacgatt gcatatcaaa aacttcttaa ccctttggaa
     8101 acattggtac cgcgcaaacc cgcagaatct ttgcaggaat atagcctgcg aatagaacaa
     8161 gaattcaagc tagatctgcg tttcattcga ttaactactc aatatgaagc agaaatatat
     8221 ggacgacaag cagatccggt aaaattcgat aaaaaaacct atttagaaat aacgcatata
     8281 ataagaaaaa tggtatacaa aaaaattctg acaaaaggga gccgcttcaa aaaataagat
     8341 aaaatttctg gagaatctac ttctggaatg ccgagaacca gctgattgag tatcgattac
     8401 tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt atctgatttt
     8461 caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac
     8521 tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag
     8581 aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc
     8641 tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga
     8701 atcgattgcg atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact
     8761 tggcgcatca ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt
     8821 ctggacaaca gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa
     8881 ataatctgct actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat
     8941 ttttctttct ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa
     9001 agcaacgatt gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc
     9061 ttggtaaggt ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc
     9121 attaactatt ttcacttgtt gttttttgac tgttgcctct agcccgactc ctgaaagatt
     9181 ttttagattt tcagcttgat aaggcttgat ttcatgttct ttcaaataat tcataatgcc
     9241 aacagccaat ggatgattgg cattcgcttc caaggctcct agatattgaa ggatttcatt
     9301 tttattgaac gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag
     9361 tgttcctgtt ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg
     9421 gtttttcaaa agtaatccgt ttttagctgc tattgatgtg gaacgagcaa caactaatgg
     9481 gatagctaaa cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa
     9541 tgcattagga agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa
     9601 tgcgacataa aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc
     9661 tccttgtgct tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc
     9721 agttacttcg atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt
     9781 ggcttgtttt ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc
     9841 acctttcaag attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc
     9901 accttcgttt acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt
     9961 cactgattct ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc
    10021 attcatttca atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa
    10081 gaaatccatg acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata
    10141 aatatatgca actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt
    10201 caattccatc ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc
    10261 taagatcaat acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc
    10321 catcattggt gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg
    10381 tttgaaattc cccatgtgca tggaatgatc catgccgccg tgttcgtgat ccatgtgcat
    10441 cgaatggtcc atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga
    10501 atgatccatc tgactatggt catgtttcat ctccatctgt ttttcatgag attctgtact
    10561 ttctttgttt actacttctt ttttatgctc atgttttttc tccatatcgt tcccattcat
    10621 ttttactcca tcccttctct tactttttca tgtgtcgatt taagcacagg gagttcaaca
    10681 atacactgac ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta
    10741 gtgcggcaaa aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga
    10801 tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc aaactgctat
    10861 tcatcagcgt cacatcggct gtttccatcg caatgtccgt tccacttccc attgccatac
    10921 cgacatcagc taatgctaag gccggtgcat cattgattcc atcgcctgcc ataccgactt
    10981 ttttgccatc tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag
    11041 caaagatatg ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt
    11101 tgtctcctgt cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt
    11161 tgacttctag tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag
    11221 ctaaaaacat gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat
    11281 caaatgatag gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt
    11341 taatcgtacc gctgattcca gcacctgggt gggctgtaaa atcaatcata tcgtatgttg
    11401 ctgcttcttg cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt
    11461 gttctaaagt ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg
    11521 gctttccttc tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag tgagcagctg
    11581 cttctaatgc ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta
    11641 ccataatagc tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag
    11701 agacactatg gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg
    11761 tgataaacgc gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt
    11821 tttggattgg ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata
    11881 ctgtctgctt gccgacttgt gtaacttcca tgtgaagtag accattcgta ttgattgtcc
    11941 cgccaaagag cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac
    12001 tttcatctat cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag
    12061 gacggatgac tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc
    12121 cgtttcttat cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac
    12181 ctgttttcgt ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca
    12241 tactgctgct ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc
    12301 tcaatgcaaa ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg
    12361 tcttgattgc atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag
    12421 cagacagcac taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc
    12481 ctaacatcat agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa
    12541 gatcgaatag catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg
    12601 cttcatcgta caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag
    12661 tggtcgtccc ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga
    12721 ccacaccagg ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca
    12781 tacctgtaat gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc
    12841 tttataaccc aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc
    12901 aaacttgacc gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag
    12961 tgcgcttact gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga
    13021 aaattgttgt ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg
    13081 gatacagtta cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc
    13141 attgatatct tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat
    13201 cctttttgca cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc
    13261 cgagacagct ggataataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt
    13321 ttttaccaaa cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc
    13381 taagatctgc gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat
    13441 ctcccactct gaatcactga tctttactgg cgttttgact ttttccattt atttcacacc
    13501 tttcgtttac aaatgtaatc attatcttat gaatacagat tacacttgta aacaaaaact
    13561 gtcaattatt tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg
    13621 ttttaaggat gatcagttta tttcttgcat tagctacttg gatcagctgc tgtattttcg
    13681 gttcattgtt ttcttcaaaa tgccagataa aagataaaaa ttctaagtat tccctgttta
    13741 gtttctccga aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta
    13801 gagaacgcca aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct
    13861 tatctgcttc tttaatcctt tcctctaatg tagaagtata atttccttca acgatccatg
    13921 acggattaga taccataaac aattgctgtt cttgcaaaaa ccactttttt gcctgaatac
    13981 tgtagtctgt tgtatgccat aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt
    14041 tgatcttcct tgcgagggtc gattttcccg acccactttg tccaatcacg atgatcttca
    14101 ttcttctccc tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa
    14161 aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaa
//
LOCUS       DANKMN010000075        13941 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000075 DANKMN010000000
VERSION     DANKMN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            135..308
                     /locus_tag="PW224_002298"
     CDS             135..308
                     /locus_tag="PW224_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908955.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            948..1421
                     /locus_tag="PW224_002299"
     CDS             948..1421
                     /locus_tag="PW224_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL2908956.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1430..1657
                     /locus_tag="PW224_002300"
     CDS             1430..1657
                     /locus_tag="PW224_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2908957.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2293..2664
                     /locus_tag="PW224_002301"
     CDS             2293..2664
                     /locus_tag="PW224_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908958.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2920..3162)
                     /locus_tag="PW224_002302"
     CDS             complement(2920..3162)
                     /locus_tag="PW224_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2908959.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3194..4096)
                     /locus_tag="PW224_002303"
     CDS             complement(3194..4096)
                     /locus_tag="PW224_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2908960.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4109..4297)
                     /locus_tag="PW224_002304"
     CDS             complement(4109..4297)
                     /locus_tag="PW224_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2908961.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4311..4874)
                     /gene="amaP"
                     /locus_tag="PW224_002305"
     CDS             complement(4311..4874)
                     /gene="amaP"
                     /locus_tag="PW224_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2908962.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4912..5805)
                     /locus_tag="PW224_002306"
     CDS             complement(4912..5805)
                     /locus_tag="PW224_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2908963.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5883..6806)
                     /locus_tag="PW224_002307"
     CDS             complement(5883..6806)
                     /locus_tag="PW224_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2908964.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6855..7205)
                     /locus_tag="PW224_002308"
     CDS             complement(6855..7205)
                     /locus_tag="PW224_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2908965.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7238..8140)
                     /locus_tag="PW224_002309"
     CDS             complement(7238..8140)
                     /locus_tag="PW224_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2908966.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8133..8990)
                     /locus_tag="PW224_002310"
     CDS             complement(8133..8990)
                     /locus_tag="PW224_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2908967.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9324..10133
                     /locus_tag="PW224_002311"
     CDS             9324..10133
                     /locus_tag="PW224_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2908968.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10173..10670
                     /locus_tag="PW224_002312"
     CDS             10173..10670
                     /locus_tag="PW224_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2908969.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11055..11128)
                     /locus_tag="PW224_002313"
     tRNA            complement(11055..11128)
                     /locus_tag="PW224_002313"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11092..11094),aa:Ile,seq:cat)
     gene            11315..11638
                     /locus_tag="PW224_002314"
     CDS             11315..11638
                     /locus_tag="PW224_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908970.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11802..12056
                     /locus_tag="PW224_002315"
     CDS             11802..12056
                     /locus_tag="PW224_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908971.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12126..12371
                     /locus_tag="PW224_002316"
     CDS             12126..12371
                     /locus_tag="PW224_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2908972.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12447..12812
                     /locus_tag="PW224_002317"
     CDS             12447..12812
                     /locus_tag="PW224_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908973.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12873..13436)
                     /locus_tag="PW224_002318"
     CDS             complement(12873..13436)
                     /locus_tag="PW224_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908974.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4087 a         2578 c         2303 g         4973 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      841 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat
      901 aattattctg ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa
      961 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
     1021 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
     1081 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
     1141 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
     1201 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
     1261 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
     1321 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
     1381 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
     1441 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
     1501 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
     1561 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
     1621 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
     1681 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gcctattatt
     1741 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
     1801 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
     1861 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
     1921 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1981 gtgcttaaaa cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta
     2041 agctcccata tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta
     2101 ctatgtagtt ttgataaaat gatttgaata acagtgatgg acaattcttt tttattttta
     2161 acattatttt gataataatg attatttatt taaaaataca ttagataaca tttgttacga
     2221 cacgaaaaag cttcacaaac aaatatcgtt attttaaaat gagggcgaac tcacacaatt
     2281 aggaggaata gtatgccatt tattgtttac ttttttcttt cattactcgt gatctatatt
     2341 ccattaccaa catttttcct gctcgtcaat cgtctagcag agcaacatga tacgattgct
     2401 gctcttacga aactttgttt acttctattt gtttgtctag attactttac gagctattat
     2461 tggctaaaac agacaagagt aaaaaagaga tattatttct tccttgtctt tttcaatctc
     2521 tttgagtttc tgatccattg ctttttactt catcagtatc atacgatcca tttgtggatc
     2581 atctgggttt tcaaagtggt cgtattattt tgtatgttca cctttcctat ctcaagaaaa
     2641 tttagggagg cattgtttta ttaaagaagc ttctctgtat caataaagac acatttaagg
     2701 aaaaactttt aaaaatctaa ttgggaattt gcccgcataa ataagaggct gtgacaaaag
     2761 tcttgtcaca gcctcttatt ccgtataaac agtgttcaaa agcttgcatc ttcgataata
     2821 tgcccaacca aatgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat
     2881 gaagaacagc tttttcacga cctcttttta aaagtctttt tacagttttc gagcaaagaa
     2941 tgatacgatt agtactaaaa tgatggcacc aatcaatgat ggcacgattg ccattcctgc
     3001 aatacttggt ccccaagaac ctaataatgc ttgaccaagt gctgatccaa ctaaacctgc
     3061 tacgacattt gcaataatcc ccatcgaaga acctttattc gtgattgctc ctgcaattgc
     3121 tccgataatt cctccgacga taagtgacca aataaatccc atgtttcgtt cctcctaatg
     3181 ttttacttcc ttattactga acccgtggtt ctgtattttc ttttactttt tgtgcgccgc
     3241 ttccagctgc tgattttact ttatctgttt gattcgaagc aaattcaccg gtttttttcg
     3301 ctgcgtcagt tactttgtct tgcaccgttt cttgatcttt ttcaaattct tcttttgttt
     3361 tgatgtccac tacgttagcg ttgacttcga tcacttctaa atgtgtcata ttttttactt
     3421 ctttgccgat tacttcttgc atttcttcaa agattttttc gatatctttg ccatattcca
     3481 caatgacatc catatcaact gctacttgtt ttttaccgac ttctgtttcg atcccagccg
     3541 ttacattatc tgtattcaca agttttcctg ccacattaga gaagaaacca ccgtttgcag
     3601 ataataaacc gtctacttgt tcaattgcga ttccgatgat tttttgcaca actttatctt
     3661 cataattcag ctcaccgcgc attgaatctt cctgttcaga atcatgcttt ccttggttat
     3721 ttgtatgatc agaaccaagt tttccttgtc cgtttgtgtg gtcagaacta agtttttctt
     3781 gtgctttcgc atggtcagaa ggatgttttt gctcttccac aggtttagaa ggatcgattg
     3841 catcatgttg ttctggaact ttagattctg gagttcctgg atgatgaggt gcatgctttt
     3901 ctgcatcatg tttcttctct tctactcctt gttgaacgtt agggactact ggatctggat
     3961 gtgcagatac tggtgctgtt ttgttctttt catcgttcac accatttgat tggtttggtg
     4021 tcgttgggtc tggatgtgca gacactggtt tttggttgtt cattttattg ttattatttt
     4081 tgttattgtt attcatggat gtttcctcct aagatttaaa atagtgatct aagattttcg
     4141 tttgtttcag ataaaaaccg atagcgacgc cgatcgctgc catgatcaag atcaataagg
     4201 tcttgaaaaa gcccacggtg atccataaaa cggctaatat caatccaagt accccaaaga
     4261 tgatagggag cttgtatgtc gaaaatagtt ctttcatttt tggtcccccc ctattctact
     4321 cgtttgcggt cattgcttcg tttagctggt tcttcaaagc ctgtgtattt caccgatact
     4381 ttcactggct cttttgttcc caatatgtgt tgtacttcat tttcgatttc tttcatcaat
     4441 gttcctgttt taccaatgag tgctgaagtt cgtttcaact ccccttttac attgacggcg
     4501 atcttgtttt tcgttgcttt gacatttact tttggtgaat ggatgaattc atcttcatgc
     4561 aagtgagaac gaaccattcc ttcaattgct tttttatcca acgtcaactc cccatcacca
     4621 tgtttcagta gaaagtttcc gattgttttt ggataaaaca ggaaaacaag taacgtgata
     4681 attgtcaaaa tagcaaatgc aacggcaagc caaaatacat attgttgcac gtatgaatta
     4741 gtgatggaaa agaatcgaaa cttctctagc tgaaaaggca atggataggt tgcacttaat
     4801 agtatcgcaa acaaaagcac agatagcaaa agtaatgttc cgatcactcc gatcgttttt
     4861 acacctttat tcattttcgc acctcctcat aggggtgaaa cgatcaactt cttataagtt
     4921 gatcgcttct ccaccagtta ctccgtaaat ttgggctgta acataacttg ctttattcga
     4981 tgctaagaaa acataaactg gtgctaattc tcctggctga cctgcacgtt ctagtaaact
     5041 ttgctgacca aattcaggga tcgaatcaga aggttgtcct tcgtctagtt gtaatggtgt
     5101 ccagattgga ccaggtgcta caccatttac acggattcct ttaggtgcaa gctgttttgc
     5161 taaaccgatc gtaaagttag cgattgaagc ttttgttgct gcgtaatcta ataaatgttc
     5221 gcttggattg aatgcttgaa cagaagttgt tgttacgatt gctcctcctg ctgggatatg
     5281 cggaacagct tctttcacta aagcaaacat agcgatgatg tttatcataa atgtgtcttt
     5341 gacttgctca agtggtaact cagcaataga ctgacaagaa atttgctgtc ctgcgtttag
     5401 taccatcgta tctagcccgc caagttcact cacggcttta tctatgattt ctttaggtgc
     5461 ttgttcgtca cgaagatcaa aaggtagtaa gacaatctta cgtcctgcat ctttcacgta
     5521 ttttgctact tcctctgcat ctttttcttc tcctgggaag aattgaagag cgatatctgc
     5581 cccttctcta gcaaatgcaa tcgctgctgc acgaccgatc cctgaatctc caccagtgat
     5641 tagtacacgg cggttttcta gttgattatt gccttcatag gattcttctc cacaatcagg
     5701 aacaggaatc atattctgct gtaaagctgg tgtatcctga tcttgtttag gaaatttctc
     5761 cgtatggtac aagtttcgcg ggtcgattaa ttttggttct gtcattgctg tattcctcct
     5821 agtttttaaa ttgtattgcc aaaacaaatt tctttgtttg atacctgttt taaatagatt
     5881 ctttaatttg tataatcttt ccggtataaa tggtaaccta aaactactgc tgctatcaaa
     5941 gagccaaaga ctacgagtat aaagcctaga gcagaacttt tacccggtaa tcctcctgta
     6001 ttcattcccc aaatccctgc gaacaacgcc ggaacggaaa tgattaatgc agctgaatct
     6061 agatatttca tgatatgatt tagattattg tccatcatcc cgttgaatag atcaccaata
     6121 ctctctaata aatctcgata gatctcgatc attttttctg tttgtcgctg tctcaaatgg
     6181 acatcataga gtagctgttc atcgtctaaa cgtttgatga aagtttcaga attccataat
     6241 tgatccattg tttcccgttg atctcttaag gtatgatcga tatatacgat gtctctttca
     6301 agatctgcct gtttatacag ttcttcattt tccgttgttt tacgagctgc tttatccaaa
     6361 gcgtcaattc gctttttcat ctctcttagt tctttcacgt aatgggtgta aatatctaat
     6421 aatgtatacg tgatgatctt ttcaaacgag tttaaatgat ttccatgctt tgtcagcagc
     6481 cgatccacaa attcggtttt tctatcgata caagtaatca agagattatc ggaaagtaca
     6541 aaagatattg gcgttaattt tttttcgata cgattatctg actgatctag attaatcact
     6601 accaaagaaa atggatgctc tagtttcgtc tcataaaggc gctccatccg agaaacttct
     6661 tccggatatt cggttcctac gaaaatatca tcaggaagct cgaattcttc gattgcttgc
     6721 tcaatctctt cgggctttgc actatcaagt acgagccaat tatatttttt ttcatctgtt
     6781 tctattacat tcttttcttc atccacaaag tagtatctca atgtatacct cctttttgga
     6841 ctttctcgaa actattagct gaagaagcag ttctttaatt tgatattata atcgagtact
     6901 gcgatttcat tttctaatga aaagctgtca agctgtttgt tgaagtccat gttcaatcgt
     6961 ttaccgaaat aattttgctc taatttagat gtatagatgt ccaataatgg atgctcgatt
     7021 ttagtcgcat attctccaaa ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt
     7081 gcaaaagcac ctttgtccct tagaaatgga tcggaatcat tgataagcgc gagtgcgatt
     7141 tttgtatttt ccccaacctt ttcaattaac gtctctactg catttttaga cattgtaatt
     7201 tccattccta ctccccctct tcagttgttg tcttctatta tttaacaact acatctgttt
     7261 ttggttgaac ttttgcatat tttggatatt tagggaattt agctacacaa cggaaagccg
     7321 tcatccctgc atatttttcg taatatttct tcgttggccc ttcaaaatcc ttgctccacg
     7381 gtttcacatg tcctgaaaaa tggataatgt aaggattgtt gcgagtttct tcgtattcac
     7441 gttcacctgc tgctgttgga tgtttttttg cttttgccat gatatagcct tgtgcattcc
     7501 aacgcggatg aagtggtaac caacggtcat gtaagatagc atttaatgca tcttggtcat
     7561 ggaatcgtaa tttttctgga ttatgttcga tgaaatctaa tactttttgc gtaatatttt
     7621 catctaacca ttttttcaca ttgatcagca tcaaacctga gttgaaataa cgcattgatt
     7681 tagctgaaat tttcattttt tccagtcgtt gatggaaccc agcgtcttca acagctgcta
     7741 cgattgaatc accgaagtca agacgccata atttagagat atcttgtaaa gcgatcatgt
     7801 cgcaatccat atataagata cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa
     7861 tacgataata agctgtttct ggaatacgat cgctgattac cacatttgta aagaattctt
     7921 tattgatttt caagaattcc acatctgagt tcattcgtgc gtttttcact gagaagcgta
     7981 aaagctgttt actttcaaaa tcgatatcat cgtcaatcac atagaaccga acaaatttag
     8041 acgggttgca attatctaat actgagacaa ataatgcagc taaatgtggt acgaattttg
     8101 tgttacaact tgcgactact gctatttctt ttttattcat tgatctcgcc tactttcgtt
     8161 agagtactgt gtgctaattt ttttaagtat aaattggtat acggatgatc tttcaacgta
     8221 ttccaaggtt tatcatgtcc tgtaaagtga acgatcgaag gtttttcatt tccttcttta
     8281 tactgacgtt catatttttc atttggtgct ggatgacgtt caaaaatcag cgaagtctgc
     8341 atgttccatt ttggatgaag ttgttcccaa tcttcataaa gtacagcatt caaagcatct
     8401 tggtcgtgat agatgattcg atcgccattt tcactgaggt aatggatagt tttttctgta
     8461 atttcttttt tattccattg atcaatgtca atcaccatca ctcctgaatt gaagtacaag
     8521 tcatcggaat cgattcctaa tctttccaaa gctttcactt ggcctggatc gataatagcg
     8581 ccgattgttt ttccatttaa cggctcatca tatagtttta cgatatcgtc tagtactaac
     8641 acatctgaat ctaagtaaag gactttctta taatcctctt tagctaataa gtttggtaaa
     8701 gaaattctta agtaagcggt agttgtgata tggtcactga ctaggaagtc ttcgtagata
     8761 tctttttcca ctgtcaggaa ttggatactt gcattacttg aatatttatt taccgtttct
     8821 tccaatcctt ctttactata ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt
     8881 cttgttttat tgcaattttc taatgcagtt gcgatcatca cgctcaagta aggagcataa
     8941 ttttcgtctg aagcagttac cacaggaact acgccgtatc tcttctccat caaaatcact
     9001 cctttttcta atctttttct aacctgttct acatctagaa tattcctcat ttggttttaa
     9061 agcaaaatat atgctttgtt ttcattgaat attttaaaat ttttttatca caaaacgcat
     9121 cggtaagcgc taactattcg tttttcatgg ctttttattc aaaggtattg accaaaaact
     9181 actatatata gccacctttt tcttaagaat gttttttgaa aaaattcata atgacaaagc
     9241 aagttagttt cattttttta aattctcatt taatcttaag gtaacaggaa aatagatatg
     9301 taaaaagaaa gtaggaagat aaaatgaaac tagcagcgat tgatttagac ggtaccttgc
     9361 ttgatcgaaa tggaaagata ccagaagaaa atatccaagc acttcggaaa tttgatgaaa
     9421 atggcggaat cgtaaccgtc gctaccggaa ggaacagcat ttctgctaaa gatatttttt
     9481 ccgaattagg aatcggtggc tatttgattt cttccaatgg ttcactgatt gctgaaatga
     9541 aggatggaaa aatggcgaaa gttctcagac gttcaaaaat agatagcgat acactgagaa
     9601 aagccttctt ctatgctcaa gaagcgaaaa tatccatcat tgctagccga gaaacccaag
     9661 atgatcagct gacattcgat gaatcaaatc tagtcaaaga tgacccttat tatgaacatt
     9721 tcaatttaca gaaccattct tttgatcaaa ttttaaatat gttggaagat ccttctctta
     9781 gctatcttaa attagcatta acagataaga gtgaagaaaa actacagaag ctacaaagag
     9841 atctgcaagc agatggtatc caatcagtat tctctgatcc gcatttctta gaaatcacgc
     9901 ctacaggtat cactaaagct cattcacttg tttttctggc ggattacctt ggaatcgaat
     9961 cgagtgacat catggctttt ggcgatcaag aaaacgacat tgctatgttg aattacagcg
    10021 ggttgagtat tgcgatgggg aatgcacagc gacaagtaaa agaaattgca aaccaagtaa
    10081 cactgacgaa tgaagaagct ggtgttgctt atattttaaa aaacttctat taaaaagaat
    10141 aaaatgacga tatgaatgta ggaggaaaga aaatgttagt tattattgat atgcaaaatc
    10201 atattcttga tccaaacagc gaattttata ttccagagtc agatcaattg gcagaacgta
    10261 ttgctcaaag attagctaaa gcacgccaaa ataatgaata tattctcttc acacgggata
    10321 taccagttga actgaaggac aaagaagaag aacgagaaga tttgcaattg atccctcttc
    10381 tttctccaca agaaaatgaa cgagtgatta aaaagtatta ctttacgatt ccacctgaaa
    10441 ctttagctga aatccaggaa actttatttg aaagcaaaga agaacaaaaa gagatcgaag
    10501 tagtcgggat tgaaacaaac ttgtgtgtac tctctaatct gataggcctg cagagtgctt
    10561 ttcctgaagc agactttttt gttgatccaa tgttagtctc cagtagaaaa cacggagaat
    10621 ccgctttaga gctgttgaaa gatttcaacg tctctatcgt cgaatcataa aaatatctat
    10681 cttcatttac gatatttaac acacaataaa aaaagcacgc agaactgttt tagtcctacg
    10741 tgctttttct atcaaagtag attggctttt ttatgacaca gagtgaaacg gcaggctcac
    10801 gacctctttc ttaaatggtg ttcaatcagc tgaccctcgg tattagctta acaaacggca
    10861 aaatatgagg aactattttt ccgtttgtta agctaatact caggtcaaaa cgctgatttc
    10921 acaacctctt ccttaaacgg tattcaaaag cttgcatctg cagtaataag aacagccaag
    10981 taaaaacata agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
    11041 ttcacaacct cttatgtacc ttgtaggact cgaacctacg accggacggt tatgagccgt
    11101 ctgctctaac caactgagct aaaggtacat cataaataaa atttattttt gttacttttc
    11161 aactatagca cgaatgacaa actctcacaa gtttcaaaat ataagtaaaa taatattgtt
    11221 attcttattt cttttacttg tgtcataact attttgttac tttaattttt atttgcttat
    11281 tttagaaaaa atgttattct tttatttatt ctcaatgtta ggagtgatta ttttgccatt
    11341 tattgcgatt ttattcgatt tagttgcggc agcagcttat tttctccaat taaattatca
    11401 aacctcagga attctgttta ttggaatgat ttttcaaggt gtgattaccc ttttgcttct
    11461 tgtcatgatg ctcagctata aaggaaaaag atatgcccgt attcaaacaa aagtatttgt
    11521 taaatatgtt tccattcgct atgggatcat cattctttcc tttttagtga atgcgatcgt
    11581 cctcttttta tatgtattga attacttaga tatcaatcca ttgatttttt ctcgatagat
    11641 aagacacatt cagcggaaca gttcctgctt atcagcagaa aactgttctg ctttttcatt
    11701 tttcattgat ctattcttct tttattttcc tagcatttca tttgtgatgt atgcaataac
    11761 atgttatctt ttattatatg aatcgttgag gagtccgtta catgattacg ttggtgagag
    11821 ttttgttttg ggtgcctgcg gtcgcactgg tcgcaagtat tgtttatctt atgaattgga
    11881 acaaagaacg gttttattta gctattttga ctcttccggc tatctacttt atgtggaaag
    11941 tttttaatta caactacttt gaacccgata gtgtttttat agaagaatta tcaggactgg
    12001 ttttgtcttt attgatcgtc attctttatt tgatacgttt aaataaaaaa cattgagtac
    12061 tattgattgc atgtgtagct ttctatagtc attataacta ttctactata atgaaaggtg
    12121 attaaatgag tatcgaagaa gctattttac tggatacttt acgttgtcgt ctgaatttct
    12181 taagaactgg ccgatcttta tctcaagtag ctgtttctag cgcgttaagt attcctttat
    12241 tcaaatatcg taagtacgaa gaaggagaag aaattccaag catcattgaa ttaatctata
    12301 tcgctgactt ttacgatatt tccgttgatt acctaattgg tcgctcagaa acagcaagca
    12361 aactgaagtg attttttcca gccttggtgc tggttttttc atttaacgtc taaggttaac
    12421 aatctatata taaaggagac atttctatgc gattatggca tgaaaaattg atttcaaagc
    12481 ttcctcgtca acagctttta ggacaacatc gggaatgctg tgcattaaga ggaaatggct
    12541 ggggaaagaa acatgctact gtggattatg tttttcacta ttctccttat agattattcc
    12601 aatatcatca gcttgtcatg gatgaaatgg aaaaacgtgg ttacagccca gcaccagagt
    12661 ggaaaaatcc tatgtatcgt ggcaaatcat gcgaagcatt ttttgattta cctactgtag
    12721 agcgcaacgt gccgatttat ccggaacacg atgaagttta tctagcagaa tgtttagcaa
    12781 atttggagca aaaaggaatt tatcttacct aatataacta gaaaagcctt ctatctcctc
    12841 ttacttcatt aaaaagcttt aaccatctta gatcaagcta aaatgtcgta agttttaggg
    12901 ttatcttcat cctcacgtgc aaaactaaaa gcgtactcta gttttggttc atcgccaact
    12961 tccctagaca taaatgaaca tctcctgacg tggtttaaaa gtaccgcatc ttcgacgtct
    13021 gtatacgatg cacagcacag attgcttctt ttgatctttc ttttgatttt tctgttgctt
    13081 cttctacacc ttcttcgatt actacatcga tgcttacaat tgcttctgcc taatattccc
    13141 atgcatcgtc gatttaagcc tctgtagtca ctgtgatcgt ttccacattt taactcctct
    13201 gtcgtggctt cagttgattt tgtgatcagt tcagttgcta agccagagtc agaacaacct
    13261 gccattgcta gcgttaatat tgatagtaaa aatagttttt ttatcataag gttccccccc
    13321 tgaaaataga ctaaaataat aacgctttct ttttttgttt tcagacgaaa attaataatt
    13381 tctaaatatt ctttaaaaaa gcttataact attatgcctt ttttaatata taacacagtt
    13441 atttttaaaa ataagaatgc taaatcactc tacaataacg gcgaaagcac aaaaaataaa
    13501 aaaattatta cgttattaaa aaaacagatg aaagatcagg ttttattaga tctcattctt
    13561 tcatcacttg aagggattta atgaactact ttttcctctt tgtcgttttt atatattcgt
    13621 attccttcaa acagtaggat agcctctttc aatgcttgat atttgaactg tccgttactt
    13681 aaaaatttag atgtgccgcc ctcttcctag aacatattct tttttacttt ttcattaatt
    13741 gacagaccaa tcgaacgaaa ctgtaaattt cgataggata atgattggtc aaactcattc
    13801 attttcaaga catctcgatt catattcaag ataggaatct ttttatcaac atcactttat
    13861 tcgtattttc ctctaccata aaagcttcac gctccacaaa attgttttat acactactat
    13921 tcagcccatt caacgaccaa t
//
LOCUS       DANKMN010000076        13933 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000076 DANKMN010000000
VERSION     DANKMN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(99..1229)
                     /locus_tag="PW224_002319"
     CDS             complement(99..1229)
                     /locus_tag="PW224_002319"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL2908975.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="PW224_002320"
     CDS             complement(1878..3080)
                     /locus_tag="PW224_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2908976.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="PW224_002321"
     CDS             complement(3140..3343)
                     /locus_tag="PW224_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL2908977.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="PW224_002322"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="PW224_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="PW224_002323"
     CDS             4880..5692
                     /locus_tag="PW224_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2908978.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="PW224_002324"
     CDS             complement(6716..6868)
                     /locus_tag="PW224_002324"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2908979.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="PW224_002325"
     CDS             complement(7059..7937)
                     /locus_tag="PW224_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2908980.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="PW224_002326"
     CDS             complement(7976..9094)
                     /locus_tag="PW224_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2908981.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="PW224_002327"
     CDS             complement(9091..11190)
                     /locus_tag="PW224_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL2908982.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="PW224_002328"
     CDS             complement(11241..12062)
                     /locus_tag="PW224_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2908983.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="PW224_002329"
     CDS             complement(12059..12868)
                     /locus_tag="PW224_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2908984.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="PW224_002330"
     CDS             complement(12889..13383)
                     /locus_tag="PW224_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2908985.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="PW224_002331"
     CDS             complement(13395..13883)
                     /locus_tag="PW224_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2908986.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
BASE COUNT         4236 a         3019 c         2250 g         4428 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaa
//
LOCUS       DANKMN010000077        13899 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000077 DANKMN010000000
VERSION     DANKMN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="PW224_002332"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="PW224_002332"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL2908987.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="PW224_002333"
     CDS             complement(2262..2513)
                     /locus_tag="PW224_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL2908988.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="PW224_002334"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="PW224_002334"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL2908989.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="PW224_002335"
     CDS             3463..3681
                     /locus_tag="PW224_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2908990.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="PW224_002336"
     CDS             complement(3970..4482)
                     /locus_tag="PW224_002336"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL2908991.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="PW224_002337"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="PW224_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL2908992.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="PW224_002338"
     CDS             complement(6935..7378)
                     /locus_tag="PW224_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL2908993.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="PW224_002339"
     CDS             complement(7379..8167)
                     /locus_tag="PW224_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2908994.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="PW224_002340"
     CDS             complement(8164..9105)
                     /locus_tag="PW224_002340"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL2908995.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="PW224_002341"
     CDS             complement(9282..9869)
                     /locus_tag="PW224_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL2908996.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="PW224_002342"
     CDS             complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="PW224_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL2908997.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="PW224_002343"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="PW224_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2908998.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="PW224_002344"
     CDS             complement(12786..13721)
                     /locus_tag="PW224_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2908999.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3848 a         3107 c         2452 g         4492 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataacc cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac
    13861 tcaaattgta tttccgacga aagaaaacaa aggagtgta
//
LOCUS       DANKMN010000078        13692 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000078 DANKMN010000000
VERSION     DANKMN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            93..1520
                     /locus_tag="PW224_002345"
     CDS             93..1520
                     /locus_tag="PW224_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2909000.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1612..1977)
                     /locus_tag="PW224_002346"
     CDS             complement(1612..1977)
                     /locus_tag="PW224_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2909001.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1974..2339)
                     /locus_tag="PW224_002347"
     CDS             complement(1974..2339)
                     /locus_tag="PW224_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2909002.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2531..2779)
                     /locus_tag="PW224_002348"
     CDS             complement(2531..2779)
                     /locus_tag="PW224_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909003.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2778..2906
                     /locus_tag="PW224_002349"
     CDS             2778..2906
                     /locus_tag="PW224_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL2909004.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2963..4657
                     /locus_tag="PW224_002350"
     CDS             2963..4657
                     /locus_tag="PW224_002350"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL2909005.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4661..4780
                     /locus_tag="PW224_002351"
                     /pseudo
     CDS             4661..4780
                     /locus_tag="PW224_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4757..5053
                     /locus_tag="PW224_002352"
     CDS             4757..5053
                     /locus_tag="PW224_002352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909006.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5431..6564
                     /locus_tag="PW224_002353"
     CDS             5431..6564
                     /locus_tag="PW224_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL2909007.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6595..6915
                     /locus_tag="PW224_002354"
     CDS             6595..6915
                     /locus_tag="PW224_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL2909008.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6944..7408)
                     /locus_tag="PW224_002355"
     CDS             complement(6944..7408)
                     /locus_tag="PW224_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2909009.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7539..8792)
                     /locus_tag="PW224_002356"
     CDS             complement(7539..8792)
                     /locus_tag="PW224_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2909010.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8821..9138)
                     /locus_tag="PW224_002357"
     CDS             complement(8821..9138)
                     /locus_tag="PW224_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2909011.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9164..9484)
                     /locus_tag="PW224_002358"
     CDS             complement(9164..9484)
                     /locus_tag="PW224_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2909012.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9948..11363)
                     /locus_tag="PW224_002359"
     CDS             complement(9948..11363)
                     /locus_tag="PW224_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2909013.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11380..12696)
                     /locus_tag="PW224_002360"
     CDS             complement(11380..12696)
                     /locus_tag="PW224_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2909014.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12846..13544)
                     /locus_tag="PW224_002361"
     CDS             complement(12846..13544)
                     /locus_tag="PW224_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL2909015.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4347 a         2782 c         2357 g         4206 t
ORIGIN      
        1 gctttttcag aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat
       61 tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata
      121 ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa
      181 agctctccaa tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa
      241 aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat
      301 atttttcaga acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt
      361 ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg
      421 aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt
      481 ttaatgactg cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac
      541 gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg
      601 aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat
      661 tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc
      721 agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta
      781 tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag
      841 aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc
      901 agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa
      961 taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac
     1021 cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca
     1081 tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga
     1141 caattttttc cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca
     1201 tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta
     1261 tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg
     1321 taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga
     1381 agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg
     1441 cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt
     1501 cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt
     1561 gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt
     1621 ttggacatct acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac
     1681 taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat
     1741 cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat
     1801 agcattgttc tcgctgactc ggtcgctttg cacttttgta tggacaaata aatatttcaa
     1861 aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag
     1921 tcccagtaca agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct
     1981 acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg
     2041 actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata
     2101 actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct
     2161 agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga
     2221 gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa
     2281 caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata
     2341 tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt
     2401 gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact
     2461 ctcccatttt tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt
     2521 aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg
     2581 tacccaaatt tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc
     2641 tatttttatt tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt
     2701 ctcgtcctat catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa
     2761 acaaggaggc gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc
     2821 ttgcccttgg cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt
     2881 tatatgatga attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt
     2941 ggaagacatt ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat
     3001 tgccaatatg gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat
     3061 caccttttat tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt
     3121 ggatgaatat tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg
     3181 cggtcgcatg cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc
     3241 ttctgttact gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca
     3301 ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa
     3361 actgggattc aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc
     3421 agaagaagaa aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt
     3481 ttttgaaaca aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc
     3541 tgctcaagaa ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca
     3601 tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat
     3661 cgagtacttg aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga
     3721 attcaaagaa acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac
     3781 actgactcaa gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa
     3841 tgggtccatt acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat
     3901 ggattatggt gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg
     3961 aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac
     4021 gacgaaaaac catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa
     4081 tgccttgaat tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa
     4141 tatgtctatc gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt
     4201 tatttggggt tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat
     4261 taaaatgaat ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc
     4321 tggaccagtg aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat
     4381 ccctgtctat atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc
     4441 aaaagtcatt cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc
     4501 ttatcagatg atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca
     4561 ttttgcgggt attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc
     4621 tactctagct cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc
     4681 cctgcatcaa gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt
     4741 ggacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt gtgaaaaagc
     4801 tgccctgcat caagtaataa gaacacccaa gctaaaactg cttcttatgt ttttgacttg
     4861 gttggactta ttaccgcaga tgcaagcttt tgaacaccat ttaccaagag gttatgaaaa
     4921 agctgccctg catcaagtaa taagagcagc caagctaaaa cagcttctta tgtttttgac
     4981 ttggttagac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga
     5041 aaaaggcgtt tagtttcgag tatgaagaaa aattttgtga aaatggttcc tcacgttttt
     5101 gcataaattt gacttaatac cgagaaactg atcccttctg tcataacctc tttttattta
     5161 tgtctgtttc tttttgaaaa caactatttt ttcaacgttt actctcttgc caatccaaca
     5221 tcatctaacg gatacaacta tttcaatgat taccattaca ctaaaaaatc ggaaacacac
     5281 ctctccgaac acaaatacat aagactatag aatgtttcat ttaagaaaaa aagcgtttca
     5341 ttatcaaaaa ggtaagatct cctttaaaat gaaattatct gtagtactaa taatgcagac
     5401 aattttatga atctgaaagg agcatctcca atgaaagcag tcacttggca aggaaaacag
     5461 aaaatggaaa tcagaaacgt agatgatcca aaaatattag aaccaacaga tgcaatcatc
     5521 cgtatcacag caacagccat ttgcggctcc gatctgcatc tttaccatca tggtgaagca
     5581 gttttggaaa aagggtatgt tgtaggacac gaaccaatgg gaatcgttga agaagtggga
     5641 tctcaagttc aaacattgaa aaaagggcaa cgtgtggtta tcccttttaa tattagttgt
     5701 ggtcattgtc atttctgcct gaatcacatg gaaagccaat gtgatgaatc aaatccagat
     5761 cagttatatg gcgggctttt cggatttggc aagttgaacg gtaatcattg gggtggtcaa
     5821 gcggaatatc taagagttcc ttatgcagac tctacttcat ttgttgtacc agataatgat
     5881 cttcctgatg aaaaagtttt atttctatca gatattttgc ccacagctta ttggagcgtt
     5941 gaaaacgcag gagtaaaaaa aggcgataca gttgtgattc taggttctgg tccagtcggt
     6001 ctattcgctc aaaaattcgc tgttatggct ggagcagagc gcgtaatcgc agtagatcca
     6061 gtacagcacc gtctagataa agcggctaat tacaatgggg ttgaaactta tttcttagaa
     6121 gacacggcac aagctggaac agaattgtat gaactggcta aaggtggcgc agatgtcatt
     6181 atcgattgtg taggaatgga cggattagag cctgtcaaag aaaaagcgaa aaatttagta
     6241 agcttacaaa gcggcacaat ctctccatta cagatggcat cgcaagctgt caaaaaattt
     6301 ggaacagtcc aagtaactgg tgtttatatg actccagctt cttcttaccc gctgcaagaa
     6361 ttttttatgc gggatatcgc tgtcaagcat ggacaggcac caattatcca tctaatgcca
     6421 aaaatttacg caatgattgc agaaggacta ttcgatccta cacaaatcat tactcattcg
     6481 ctgccactat cagaagcagc acaagcatat gagatctttg acaaaaaaga agataaaaat
     6541 ataaaagttg ttttgaaacc ttgaattcag taaaaaatac aggtgatttt ctttatgccc
     6601 aaaatcaaac gaaataccca tgagatcgaa ataggaaaag aagaagatat cgaaatacga
     6661 ggacgtcgct tccgcttcta tttccaaaat cgttacacat tgatctcact ggctgtcgat
     6721 ttattaaccg ggattttcta tatttttgga agccttgctt ccttaactaa aatccccgat
     6781 cgatatggca cctattttta tttggctggt gctattttct tgacgattcg tcctattctc
     6841 agaatcgtaa ggaacatttt tatttacgac gaaaaaaaag caatgaagag aacaagcaaa
     6901 aaaaaagagg catgagaatt tttcaaatct catgcctctt ttttcaccct atcgtatcac
     6961 tttgatacag acgagcggtc gtcccacttt cttccttgac cattgtcaaa aatgtccact
     7021 cgtatcgttc ataaaacgtg gtatgatctg taattaagta aagtttttcg tacccaagtt
     7081 gatgcatttc ttttctgatt tccgctaata tccgcttggc tatcccctgc ttacgctttt
     7141 ccggttcaac aaacagggca caaagatttg gtgtcagatc agggcggtta tggaagtcgt
     7201 tttcaatgac gcccgctcct ccgacgatgg tttctttttc atctaagacc acataccatt
     7261 gaggaatact ttccttatta gcgattccag tacgaatact ttcctcatac acaacagcag
     7321 gaactcccca ttttgatgaa aaccaattcg cgctttgttt tactaattct gggtgttctc
     7381 ttaatttgat ccagttatat gtaaccaatg tttaaacctc cgactaaaat aaaacgttta
     7441 caaaaattat acaagtcttt atgaaaatta gcaatgacaa caatagtgat gtaaaaaaaa
     7501 aacggaaaac aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt
     7561 cttctttctt ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc
     7621 aaattgacag cggctagtac accacctgca atactttttg ttgccaatac accgccaata
     7681 attggtggag ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc
     7741 gcaaagtaag caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc
     7801 gggttcaaca cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc
     7861 gcaatcgaca agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata
     7921 gctaacagta aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa
     7981 ggtttgttca cgataaacgg tgcgctatgc ccagctgcga ttgcattaac gtttgcttca
     8041 atggctggcg cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca
     8101 aagaaccata agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca
     8161 agtcccataa atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg
     8221 aatgcagcaa aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt
     8281 aatgaagcaa atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat
     8341 ttttcatttc cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca
     8401 ataaacaaac cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa
     8461 gaaatcacac ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc
     8521 aagaaagcaa tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat
     8581 cctttccatg attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt
     8641 aagttattga tcattactgc taatgctcct aagatcatta acggcatact caccataaat
     8701 gagtcccgaa tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa
     8761 tgtttctcca taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga
     8821 ttacgcgttc aatcgacgat acagatcaat gatctctttt gctaaatctg taaaagctat
     8881 agatgtcatc agatgatctt gactgtggac cgtcaaaagc gtgacttcaa cgtgatttcc
     8941 ttgtgcttct tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc
     9001 tgcatctttg attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc
     9061 ttccattgca tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag
     9121 atttttttga tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt
     9181 tttctgcttg gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta
     9241 catctaaagg aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta
     9301 cttgcggtcc taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg
     9361 aagcagatac agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt
     9421 ttgtgactag gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt
     9481 ccattttaaa cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac
     9541 tttaaaagcg ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac
     9601 acactaaaaa agaaagcgca cactaaacac acaaattaat ttttattacc ttttttcttt
     9661 taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca acttttttca aataaacggt
     9721 attcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agatgttgtt
     9781 tttactttgc tattcttatt acttgatgca ggacagcttt ttcacaacct ctcaataaac
     9841 ggtgttcaat cagctgaccc tgggtattag ctcaaaacgc tggattcaca acctctttta
     9901 tttcaaataa ccagtaaata caatgaacat ctacaaaaaa cttctattta tttatagatt
     9961 tcgtcaggca aatccaattc tctcttatca atcaaagtct gataccacga agcagatttt
    10021 ttcaatgttc tgcttcgttc atcgttcaat tcgattcgaa ctaatccata tcgattctta
    10081 aatgcattca ttggtgacac acagtcagtg aatgcccata acatatagcc ttcgcaattt
    10141 gcgccttctt ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa ttcgatacgg
    10201 taatcatctt gcacgattcc tttctcatcc atatactgtt cttcatattc tcttcccata
    10261 ccgttttctg ttatcagcca agggatatta tgataatttt cttttagata catggctata
    10321 tcatacatga ttttcggata gatttcccat cccctggaat tattcatttt cttcccaggt
    10381 aaatcaaaga cttcataata tcgttcagga tggaaaggtg tctcttcatt ccattgataa
    10441 cgaggggcgc gaacgcgttt aggataatat tgattgattc caaggaaatc tactgtatga
    10501 tgctgaatcg tttctaaatc ctctgttgca ggatcgaaat aaatcgattg tttttgacac
    10561 aactccagga actcttctgt gtagtatcct tttaccatcg gatcaaaaaa tactcgatta
    10621 taaaacaaat catacatata agcagctttt tggtcttctg gagaattgga tcgagcatag
    10681 accatttcag gatttaaaat acagccaatc cttccaccat aatttccgct gcggtaaatc
    10741 tctactgctt tagccgttgc taaaacttta tgataattcc acatcatcca cttttttgtg
    10801 ttttgttcat gcggccagcg aacagcatcc aaatagcaac gcgtttgggg aacgattggt
    10861 tcgttgaatg taaaccagtt ttttacccta tcaccgtaac gatcaaaggc aatttgagca
    10921 tacttagcat aaaaatcaac tacttttctt gaagaccagc catcatattt ttccaaaaga
    10981 aaagctggga cttcataatg ttccaaacaa atcatcggtt ctacgtctgc ttcgatcaaa
    11041 gagtcgatca tttcacttat atgctgagca tattcttcat ctactataca gttctcataa
    11101 tcagtaaaaa aacgagccca attgatagaa gtccgataat gtgtcagatg gatggatttc
    11161 atcagatcca catcctgctg gtattgttcc ataaaattag ttgctacggc tgggccatac
    11221 ccattatgcc aaacaaaatg ttcattttta taccagctat ctataaacga atcctgtcct
    11281 tctttttttc ctttccaacc ttccgtttgc caagcagaag atgcagcacc aagaataaaa
    11341 ttttttggaa tagtaatttt catgatttcc tcctgttttt tagatcccat cattttccac
    11401 acgatttgct acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag
    11461 ttgcgtaata actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg
    11521 cattggccaa gggaccatta caactgcttt cgccgcaaat cctaaagcgg ttgcaaaata
    11581 accaatcgtt ccagtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag
    11641 catgatcggc ataccaaaaa taactggttc attgatgttg aagatcccag gagcaaggga
    11701 taacttggtg atttgtttca tatcttcccg ttttccaact aacaatactg caatcagtaa
    11761 accgatcgtg actccagacc cgcccatact catatacata tcccaaaatg gcatagtaat
    11821 aatgttcggt acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa
    11881 aagcaacggt tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa
    11941 gacttgcgca ataaacatca atagcaaaat acccggtaac ccttgtacga caccttctaa
    12001 tgggcgttgt acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat
    12061 gataaatccc gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa
    12121 actagcagaa acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt
    12181 cctcaaccag caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc
    12241 tttcgtatct gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt
    12301 cactaattca taaacagttg gattaacaga taaataagac atcactgcga tgataccagg
    12361 gaatacacca tccactttat tcagcttggc taattcgatc cctatcagaa aaacagcata
    12421 gatggtcaag aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc
    12481 taaaaaacgc aagaaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga
    12541 aaacaatgtg ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt
    12601 tatcaccatg atatacctta aattattgat tctcgttgcc acgttcccta aaacattggc
    12661 taatttatcc ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca
    12721 tctctttttg tctattagtc agcgcttaca tttcgatgaa cagaaactat ttactcctct
    12781 tttttaccaa atataaaaca aaaattcatc tgatgtattt atcataaaat acatcagatg
    12841 aactgtcaat tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc
    12901 atatggacag tcattgcatc aaacgcagca agtgcattcc gcctttcgat ggcctgaacg
    12961 atctctgtat gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta
    13021 aacaaagcaa ttgattcatt aatgattggg ataatatgca gcaaggctac gtttccgcta
    13081 gcttctgcaa tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct
    13141 cgaaaagaag tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt
    13201 gcatattccg ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag
    13261 tctcgtatca tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc
    13321 cctcgcttat cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa
    13381 gctagagccc gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat
    13441 tcatattcat tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg
    13501 atcttgtcag tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt
    13561 tcatctgatg tattttttcg taattattat aacacgtttg aatcatctga tgattttttg
    13621 tatacaaaat tgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa
    13681 aagactagga ta
//
LOCUS       DANKMN010000079        13547 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000079 DANKMN010000000
VERSION     DANKMN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            255..1034
                     /locus_tag="PW224_002362"
     CDS             255..1034
                     /locus_tag="PW224_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909016.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1080..2024
                     /locus_tag="PW224_002363"
     CDS             1080..2024
                     /locus_tag="PW224_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2909017.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2040..2819
                     /locus_tag="PW224_002364"
     CDS             2040..2819
                     /locus_tag="PW224_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2909018.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2831..3529
                     /locus_tag="PW224_002365"
     CDS             2831..3529
                     /locus_tag="PW224_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL2909019.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3557..4321
                     /locus_tag="PW224_002366"
     CDS             3557..4321
                     /locus_tag="PW224_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL2909020.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4365..5816
                     /locus_tag="PW224_002367"
     CDS             4365..5816
                     /locus_tag="PW224_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2909021.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5848..6984
                     /locus_tag="PW224_002368"
     CDS             5848..6984
                     /locus_tag="PW224_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2909022.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6984..8090
                     /locus_tag="PW224_002369"
     CDS             6984..8090
                     /locus_tag="PW224_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2909023.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8096..9118
                     /locus_tag="PW224_002370"
     CDS             8096..9118
                     /locus_tag="PW224_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HDL2909024.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9140..10195
                     /locus_tag="PW224_002371"
     CDS             9140..10195
                     /locus_tag="PW224_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2909025.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            10813..12048
                     /locus_tag="PW224_002372"
     CDS             10813..12048
                     /locus_tag="PW224_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909026.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            12132..13220
                     /locus_tag="PW224_002373"
     CDS             12132..13220
                     /locus_tag="PW224_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909027.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            13217..>13547
                     /locus_tag="PW224_002374"
     CDS             13217..>13547
                     /locus_tag="PW224_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2909028.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQD"
BASE COUNT         4964 a         1786 c         2661 g         4136 t
ORIGIN      
        1 aacacctatg aaaattatgg aaataaaaat gaaatcgatt aatttttatc ttattaatgt
       61 tttatttact tttaaatcgt aaattgccct agtcaagaaa acagtttgtg gtatgattat
      121 atagggaaaa cttgtttaca aatatttctg tgaatatatt agtttaatta tgttttttta
      181 aataaaaagc tgtcattttt atgtaagtga attatatctt aatttatcta tcatcaatac
      241 ttaggagtga atctatggaa aagccattcg tcaagtcatt gactgctgtg gcggtaggtg
      301 caggtgctat tgcgatctgt ttgtttggat accatatcaa taatcagcgg caacatcagc
      361 aacggatcaa ttatgcagaa tctgccatca ccaatcaaaa agacaccttg acttctttat
      421 caaaagaagt agacaagctg tatagtacaa aagaaaaaat ttttctaaac cctgaaataa
      481 cagaagaaac aatcacaagt ttgtcaaata agctatcctc tgtcaaactt tcagcagatg
      541 attacgacat caaagaatca gaattgccta aagaagctgt tgcaatccaa gatgagaaga
      601 aagcaatcgt gacacagtta gatagtgcag aatcaaaact gaagatccag acagctgtca
      661 acgatttatt tactaaaaat gtatcaaact ggcagcaagc tgtagacgat gtcatcatca
      721 aagaaaaggt gacaagtgca gatgtcgctc gtgtacgtga aaatatgagc ttttttaaag
      781 atagtccttg gaaaacggtt gttatgcaat atctaggatt cgctgatatt cagatcgcgc
      841 aagtcacgca actagatcag ttattcgata cgatgttaaa ggatggtcaa gtgactgcca
      901 ccgctactta cgatcaatat ttgacggtgc tcagtcaaat agagcagatc cggaatgaaa
      961 aaataagcgc agcatacgca acaaaagcac aaactgttgc tgagcaaatg ggctatagca
     1021 atataagcta ttgacagtta aaacgttgat tcaagcaagg ttagcaaacg gaggaaacta
     1081 tggcaagacg aagaagaaaa tcaagaggga aaaaaatatt tctgattatt ttagcagttc
     1141 ttatcgtatt agtattagca attgtaggag taggagcaaa attatatctg gatctctcac
     1201 attctattga gaaaacttac gaatcagtgg aacgaaccca aggagaacga acgaaaccag
     1261 ttgatctgga caaacaagaa tctttctctg ttctattgat ggggattgat actggagatt
     1321 taggtcgggt cgatcaagga agatcagata cgatgatggt tgccacagtc agtcctgaag
     1381 acaaacagac aacgatcgtc agcattcctc gtgatacgta tgtcgacatc gtaggtcgtg
     1441 acacaaaaga caagatcaac cacgcttacg cattcggcgg agctgccatg gcgatggata
     1501 ccgtacaaaa atatgtagac atcccaatcg atcactatgt ctctatcaat atgaaagggc
     1561 taaaggaact cgttgatgca gttggtggta tcgaagtcaa taatgacctc actttctcac
     1621 aagatggcta tgactttcca atcggtaaaa ttacattgaa cggggatcag gcattatctt
     1681 attctcgtat gcgttatgaa gacccaaatg gtgattacgg tagacaaggg cgccaaagaa
     1741 agatcgtaga aggcgtagcg aaaaaagtat tgagtctaaa cggtgtcagc aactatcaat
     1801 ccgttttgaa tgctctagag tcgaatatga aaacagacat gaactttgat gaaatgaaaa
     1861 aaattgcttt agattatcgg gaggcatttg gtacgattaa gcaagatcag ctgcaaggag
     1921 aaggcttcat gcaagataat atctcctatc aaaaagtcag tgatcaagaa cttgatcgtg
     1981 tgaaaaaaga attaaaagat caaatgaatc tagagaataa ataggaaagt ggagaaaaaa
     2041 tggaagagac aatcagcttg aaagaaattt ttgaaacatt acgcaaacac ttggtaataa
     2101 tcatcatctc aatgtttgct ggcttggcca tctcaggaat cgtaacattt ttcatcttga
     2161 caccaaaata cagttcacaa gcacaattga tcgtgacact gccacaatca gacactgcaa
     2221 atgtcaatga tgtcaatacg aacctgcaaa tgatcaatac gtataaagat atgatcgtca
     2281 gtgatttagt gctcaatgaa gtcaaagatc gtttagaaac agaagacaat gtgaaaatga
     2341 ctgctgaaca aatcaaagat gccatctcag ttaatcaggc agaaaattca caaatgttct
     2401 cgattcaggc aataagtacg aatgcagtgg ctgctcaaca gatcgctaac acaacttcac
     2461 aagttttcca agagaacgca aaagatgtga tgaatgttga taaaatctcg atcatttcag
     2521 gtgcagttgc caatacgaca cctgtttcgc caaataataa attgaattta atgactggct
     2581 taatgttagg aatgatggta ggtgtaggtc tggcattctt attggaatta ctcgatcgga
     2641 cagtgaaaga tgaaaaatat gtgacagata cattaggatt cccaatctta ggaacagttc
     2701 cagaaatgtc agcgaaagaa ttgaatgctg gtatccgtaa aaaaggacca gctcagacac
     2761 aaccaaaagc tacaaacgat aaaacagcgc catcacgcag aagccgctca agagtctagg
     2821 aggaaagaaa atggcacgaa cacagaaaca aaaagcagcc caaactcatg cagtcagttt
     2881 ggtcacactc accaatccct cttcaccaat cgctgaacaa tatcgtacga ttcggacgaa
     2941 tatccagttt gcctccgcag ctggtcagca aatcaaaacg attgttgtga cttcatcagg
     3001 acctggagaa gggaaatcga ccactgcagc aaacattgcc gttgtttttg ccaaatctgg
     3061 tcaacgggtg ctgctagtcg atgcagactt acgtaagccc gtcatctata aaacgttcaa
     3121 gttgaacaac gcaagcggat taagtactgt tcttagttca tcaggaagtg tagcagatga
     3181 gatccaacga acaccagtcg acaatttgtc gattctgcca agtggaccta aaccaccaaa
     3241 tccatcagaa ctactaagtt cgccaagaat ggatcagatt ttagcagaag caagacaact
     3301 atttgatgta gtgatcttcg acatgccgcc agtcgttgcc gtaacagatg cacagatcat
     3361 gtcttcaaaa acggatggca ccttattggt cgtacgggaa aatacgtcac gaaaagaatc
     3421 attgactaaa gcaaaagaac tgttagatat ggtacaagca cgagtcttag gtgtcgtata
     3481 caacggagca gaacacagca aagatgctgg ttattactac ggaaattaag gaataagaag
     3541 agaggagaca agagaaatga tcgatctaca ttgccatatc ctaccaggaa tcgatgacgg
     3601 ggcagaaaac ttagaagcaa gtattgcaat ggctgaaaaa gcgattcagc aaggcattac
     3661 gcatatttta tgcacaccac accacaataa tggaaaatac agcaatgaaa aatcacaagt
     3721 catttctcta gtcgcttcgt tacaagccga attagagaaa agacagttgc ctttgacgtt
     3781 attggaagga caagaagtac gtattaccgg aacgctcatc gaagacatcc accgagatga
     3841 aattttattt acagatttag atgatacata tcttctgatc gaatttccga cactggaagt
     3901 gccgttatat gcagaacggc ttttttttga gttgttagaa ctaggaaaga taccagtgat
     3961 cgtccatcca gaacgcaacg cgcattttcg agaagaccca aatcatttgc ttccgttcct
     4021 agatatggga tgtttggccc aacttactgc gccaagttat gttggtgtat ttggcaaaga
     4081 tatccagaaa acagccaaaa caatggtcaa acataatctc gtgcagatgg ctgcttctga
     4141 tgcacacggg gtctctaagc gcacctttta cttaaaagag tgctatgagc agattgccaa
     4201 ggagtttggt aaggaaaaag tagtacagat gaaacaagtc gcaaaggatc tgatcaacgg
     4261 ggatgagatc aattatccaa tctatgaaga ggtaaaaaag aagaagtttg gtttatttta
     4321 gttctttatt cgtagataaa aaggaaatga ggagagagtg agagatgtat agaaaaaact
     4381 caagtggttg gtctaaacat attgatttta ttttattaga cttattttgt gtccagcttg
     4441 ctttttatat atcatatgta atgcgacagg gtgactggaa tccgtatgtg attcctattt
     4501 atcgccacat ggcaattttt atagaacttg agaatcttac gattatattt ttatttgagg
     4561 catataaaaa tgttttaaaa agaggatatt ataaagaggc tgtagcttct ataaagcaat
     4621 cttttatgct gttacttgtg tgcagtttat accttattac aatgcaggat ggaaatgatt
     4681 attccagagt ttctttattt ataatggggg tggtgtatgg attattaaca tatataattc
     4741 gtatactttg gaaagggttg ttgcatcacc gcatgaaaaa cggtgaagat gtatcgttag
     4801 ttattgtaac atctcaaaag attgcaaaaa atgtagttcg taatcttaaa gagaagaatt
     4861 atggcatgta taaacttagt ggtgtgattg tagtagacag agatatgtat ggaactacca
     4921 tcggaggtgt cccagtagtt gcaaatgctg ataatgcagc ggaatatctt ttacagaatt
     4981 gggtagatga agtattcatc aatgttgacg aaagtgttcc atatcccaaa gaattaatcg
     5041 ccagatgttc ggaaatggga cttgctgtac atatgaatct tacaaaagtg tctgatagta
     5101 cacgtggaaa acaatttatt ggccgagttg gtgattatac tgttattaca tctagtatta
     5161 atgttatgac gatgaggcaa gcatttataa agagaacgat tgacattctt gctggaatta
     5221 ttggatgtat tgcaacaggt gttattttta tcttcgtagc accagttatt tacatcaaat
     5281 caccagggcc gattttcttt gcacaagaac gtattggtca aaatggtaag acatttaaga
     5341 tgtataagtt ccgtagtatg tatatggatg cagaagaacg caaagcagag ttgatgaaag
     5401 aaaataagat gagtaataat ctcatgttta agatggattt tgacccaaga atcattggca
     5461 ataaaatact tcctgatgga acaaagaaga caggaattgg acaatttatt cgatctacat
     5521 cattggatga gtttccacaa ttttataatg tcctaatggg aaaaatgagc ctggtggggt
     5581 ttagaccttg cttgaagtca gaatatgaga agtatgattt tcatcatcgt gcgagaattg
     5641 cgatgaaacc tgggattact ggaatgtggc aggtgagtgg tagaagtgat atcacagact
     5701 tcgaagaggt tgtaaaactt gatgcagaat atatcaggaa ttggagtatg ggattagatt
     5761 ttagaatttt gtttaggaca gtcaaaactg tgcttcatag aaacggttcc atgtaagata
     5821 aagatactat agaaggagta aaacgtgatg gcaagaaaaa aattaaaaat ctgtatgcta
     5881 ggtcataaaa gaattccatc aagagaaggt ggaattgaga tagtggtaga agaattggca
     5941 acgcgaatgg ttgcacttgg gcatgaagtg acttgctata atcgaagtgg tcatcatgtt
     6001 agcggaaagg aatttgacca gcataaaaat aaagaatata aaggtgtaaa attaaagact
     6061 atttttacat taaatataaa aggaattgca gcaatgagtt cttcagtatt tggtggaatt
     6121 agagcagcat tcggaaaata tgatattgtt cattttcatg cagaaggacc atgtgctatg
     6181 ttgtggttgc caaaactttt tgggaaaaga tgtgttgcaa caattcatgg tctagatcat
     6241 caaagagaaa aatggaacaa gcttgctagt acatacatca tgttagggga aaagtgtgct
     6301 gtaaaatttg cagatgaaat aattgtactt agtgaaagcg tccaaaatta ttttgaagat
     6361 atatatggaa gaaaaacaag gtttatacca aatggcgtta aaaaaattga aataaaatca
     6421 gcaggattga ttacagaaaa gtatggatta acaaaagatt cttatatttt gtttttgggg
     6481 cgattggttc ccgaaaaagg aattcggtat ctaattgagg catttaaaga tgttcagaca
     6541 gataaaaaac ttataatagc cggaggaagc tctgatacag atgaatttgc aaatgaacta
     6601 aaggaattag caaaaggtga tgagcgaatt atatttactg gatttgtaca aggacaggaa
     6661 ttagaagaat tatatagtaa tgcgtatatc tatacacttc caagtgattt agaaggaatg
     6721 ccattaagtt tgttagaagc aatgagttat gggaactgtt gtatagtttc aaatatctca
     6781 gagtgtacgg aagtagtaga agacaaggct atgattttta agaaatcgga tgtaagtgat
     6841 ttaaagataa gattagaaga ggcatgcaat caatcggaaa tggttaaagt tttgaagaat
     6901 caagctactg agtttatttg tagtaaatat aattgggata aaatcgtaca agaaacatta
     6961 aatttgtata ggggaaatat ataatgattc gaatattaca gtgtgtgaat gatatgcatc
     7021 gtgcagggtt ggagacgatg ctaatgaact attataggaa tatagatcga actaaaattc
     7081 agtttgattt tttgacacat cgtccatata aaagtgatta tgatgaggaa ataataagtc
     7141 ttggaggaaa ggtctattat gcaccgagac tttatccaca aaattatcct aaatatttta
     7201 aatggatgga acaatttttt aaggatcatc cagaatatga gattgttcat tctcatattg
     7261 atgcgatgag ttatttacct ttaaaagctg caaaaaaagc tgggattccg atacggatag
     7321 cacatagcca taatacatcg atagatagag attttaaata tcttttaaag caatattatc
     7381 gatgtaagtt aacgtctgtt ttgacacatg aattttcatg tggaaaagta gcaggtgatt
     7441 ttttgtttcg aaatgataaa ttcactttga ttccaaatgc agtggatgct aaaaaatttt
     7501 attatgatga aagtattaga aatgatgtac gaatgaaatt aggaatttca agtcagacgt
     7561 ttgtaatggg acatattgga agaatttcat attctaaaaa tcatcgtttt ttgattgaaa
     7621 tttttaaaga atttcacgct attaagaata attccgtatt attaattatt ggaactggtg
     7681 atatggaaga agaaataaaa aattatgcaa agaaaagtgg catagatgat gatattaaat
     7741 ttttaggaaa tagaaatgat atagaaaaat tttatcaagc atttgacgta ttaatgttgc
     7801 catcattatt tgaaggggtt ccgttagtgg gtattgaagc tcaatttgca gatttaccgt
     7861 gtttcttttc ggagaaagtt cctacagaag ttgctttcag tgataagacc aatttcataa
     7921 gtttaaatca atcggcaaaa gaatgggcga gagagattgc aaatgtagat atctctcatc
     7981 gagattcaga acgttcgatt ttaataaagg cagattataa tattcaaaca gcatacaaaa
     8041 tcttagaatc aaaatattat gagttatttg aattaataca aagaggttaa agaatatgac
     8101 acagaaacat tcaagtataa aaagaattat acatcgcata tccgaagaat tgaatgcaac
     8161 aagacgaatt gcaaagataa catcttggaa ggaagcattt gccacgttac aagctaaaat
     8221 tgatattcaa gtcatgaatc gtaatggata taatgaaagt aaaaaacaga aggaacattt
     8281 attaaaaaaa catgaggtaa tgctgaagta ttatgaaaag actttttcag attttttgaa
     8341 aacatatgat tatagtcaaa atcaaataat taatcaatca aaatcaccat attctaactg
     8401 catttgggtt tgttggtggc agggattgga tcaatcgccg gaaattgtaa aagcttgtgt
     8461 aaaatcaata aaacgaaatg cagggaaaca tactgttata attttaacag aagataatta
     8521 tagacaatat gttgatatac caaaatgggt ggaagaaaag aaaaacaaag gaatcattac
     8581 gagaacaaat tattctgatt tattaagatt atctttgcta gcaaaacatg gaggtttgtg
     8641 gttagacgcc acattttatt gtacaaaacc aataccagat gagtattttg atatcgatat
     8701 ttggtctatt aggaggcctg attatgctca cgcatccgta gcatcaggat attttgcggg
     8761 ttattcatta gcatgtaatc aagagtctag atggatgttt gctataatta gagacttttt
     8821 tttaaattat tggaaaaata atgatatgat ggtggattat ttgatgatag attatatgat
     8881 tgttttagca cagagaataa atcatgaaat agcacagaca tttgataaaa tacctagtaa
     8941 caatccatta tgtgacgaat tgattaaaaa tttaaataat atatatgatg aaaaagtatg
     9001 gaacttatta aagaaagata ctttactgtt taagttgaca tggaaacaat cttttaaaaa
     9061 gcaaaaagat gataaagtta ctttttatgg gatgttggta gaaaacaaat tattataaat
     9121 attagaggta taaaaagtta tgaaacacaa ggtaagtgtg atagtaccaa tttacaatgt
     9181 tgaaaaatat ttaagaaaat gtgtggatag tttattgagt cagacattaa aagagataga
     9241 aatcgtatta gttgatgatg gttcgccaga tgcttcgggg gaaatagcag atgagtatca
     9301 aaaaaaatat tctaatgtaa aaacaataca tagagaaaat ggggggttag gaccggcaag
     9361 aaatacagga attgagaatg cgacaggaga atatgtggct tttcttgatt cagatgattg
     9421 ggtagaaagt gatatgtatg aaaaattata cttagttgcg tccaaagaaa atgctgatat
     9481 agtagttagt ggacattgtg attttgcgaa tgacaaagct atggtgaaaa agcaacatcc
     9541 tttgagtggg aatgtatatg atacgcctaa tagtattaaa aatgtacgaa aaaatttatt
     9601 tggacatagt ccaaaagatc aagaagtaga ggccttcccg atgtcagtat gtatgtcttt
     9661 gtataataaa aatctgctag ataaatatag actaagattt gaaaaaattt tatctgaaga
     9721 tacaattttt aacttgaatg catatgatgt cgcaaataaa attgctttta cagaatatac
     9781 agattattgt tatagaaaag aagaacaaga gtctattaca aaaagtttat ctccaaataa
     9841 gcagacggaa tttaaagaat ttttaataac tctaaaagct atagcatcga aagaagattt
     9901 agaatgtcaa atcagagcaa aacgtatggc aattgattat tgtaggcttt atgtgggctt
     9961 agttgatgat gcaaatttgg gaattaaaga gaaaagaaag tatataaaaa aatttattga
    10021 agatgaggga ttaattcaat gttggaaggg atatcctaca aatacgctac catttcaaca
    10081 aaaatttttt cataaaatgc ttgaatgtaa gtgttataca atattgttgt tgttaagtag
    10141 aattagaaag ataattaaaa atatgaccag aaaaaaattg tttataatat tataaactaa
    10201 aagtatatca aataatagaa attaacaagt tacaaaaaaa tattataaag aataatattt
    10261 tctaaaaagt aaactatagt aatgtggatt atcaccttcc aacagtatat aaaatgagcg
    10321 taaaaacggc tatttttgcc ttaacaacga tttttttgta tacaaatacg atatgctcat
    10381 taattattgg aatttgtagt gctgtttaaa ttaatgaagg tgtaattata tgagtgatca
    10441 ttttgaaaaa gtttgttgag aagatgtgct tctagtcatg tgatgggaga aaataattta
    10501 actgttagag tgaatagtta aattattctg gttatatgaa aaaagtagtt tctaaaataa
    10561 tcttgcaggg attaacttaa agaaattgag agaatagttt ctttgaaatt tctatataca
    10621 atgagaagct gaaatttact tttatttgat gactggaaat agaaattaaa tatattgtat
    10681 tgttacatta aagaaaactt gtatggaaaa atcagtacaa taccattatt taatgttctt
    10741 cacttaaagc gcaaaaaata tcagtttatt aaactcgtat atagaacaaa tatatgtaga
    10801 cggggcgaaa cgatgagtgt aaaaaaaatg ctatttatgg taagtacaat aattgtatta
    10861 acatcccgat tttggggaat taatactagt ttatctatgc gatattttat tatggcaata
    10921 tgggatttat attttatgtt aagctatttt tcttatcgtg agaaaacata taaaaaatac
    10981 gttatcaaga ataacttttg gttgactata atgccgtttg tgatattttc tatctatact
    11041 ctaataatat ggggaattgg ttcaaatgca gaatttgaaa attttataaa gttatgttcc
    11101 acgttgcttt atttaattct tgcatatggt tacgcatgta cagcatttta cttatttaag
    11161 gcagaaggaa ttgattttat tttttggtca ggtgttattt catatatgag tggttcagtt
    11221 ttttatttaa ttgtatctta tggtttaaat ggtttaattt cttatataaa aagtcttata
    11281 acaggtgaaa caaatactgc aaattttgct atggaggtac atgatttaac ttttgcgatg
    11341 ggggtttttt tcctttatta cgtatttttt gaaaataaaa atacaaaaaa acataagata
    11401 aaaattatcg tatctttaat tttaattttc atgggattga agagaattga actgttagct
    11461 ttaagtggtg caattctagt gtattatgtt ttattaaagt ggggaaaaaa gattcaaatc
    11521 cgtgcattag tgtgtactat ttgtgcaact gttatttcgt tagtgtttat ttacattata
    11581 gatacaggaa ttttggcatt gcttatgtca aatttgggca ttacagatga cggtgcaagg
    11641 ctttcatttt atctatattc atctaaattt tatgaacttg gtttaggata tgtaggaaca
    11701 ggatggacat ggttttcaaa atactttttt aatctttatt tatcgcactt taggttaaat
    11761 ggtggaagaa ttgcagcaag cctacacagt gatatattaa ctatttatat agaagttgga
    11821 tgtatagttt ttattatatg gatgttttgg ttattttttg tgaaaccgat aagactaact
    11881 aaaaaatttg gattgaaaac aggggagtgt gtattattat taactatata tatgtttata
    11941 ttatatttga cggataatac gctcacttat ccagacactc aaatgatttt tctcttagta
    12001 cctatgatag tttcgtgggt tgataatcaa caacgaatat cggaatagat agctaaagta
    12061 tattttaatt atgaatttat aattagttgt aaaagttaat atttaacgga caggaagtag
    12121 ggtgaaaaaa tttgaaaaaa attttagtgt tagcaaatac atattatcaa ataatagtag
    12181 caatacaaat gaaaaataca attttttccg atgagtatgt tgaaattatt atttctgatc
    12241 attctaaaaa cactaaagaa attgcgaaaa aaatagaaga aaaaaaagtg tttgatggct
    12301 gtcattattt agaaagcaaa tcttttgatg ataatattgg gtattttcaa aagattaagg
    12361 actgtcttca gatttcttgt ggtaaatcaa atcgatatgc ctattatttg aaaaactgta
    12421 aaagtgaata ttttgatgaa tttatttgtt tttcaatggg agaggtttgg ttggaaggaa
    12481 tatatgcttg cttatatcga aataataaaa atgtacaggt atcaatttta gaagaaggta
    12541 ttttaagcta tggagttgac attattccaa cagcaagaac aaaagctatc gcagtactca
    12601 attacttaaa aaaaaggact ccactatata acaaaagcta taatttttat tgtttttttc
    12661 ctaatatata tacaggaaga gagaatacaa tacaaattcc attagttaca gctgggtcaa
    12721 gaaccgcatt gcaactttcg gatattttta accctatgat agatatttat aaagaaaaaa
    12781 aatatatatt ttttacgtct gtttatgatt ttgagggcgg agaacctgtc ggagaatatg
    12841 aattagtatg caaaatagca gacttagtag gaaaagataa tctaattgtt aagacgcatc
    12901 ctagagatgt aagaacaatt tataaagata atggatttat cgaggataaa aattcatcaa
    12961 taccttggga ggcgattcaa ttgtcaggcg attttaacga taaaatattt atgacaatta
    13021 attcaggaag tgttatttca ggtagtacaa tgtctgcaaa acctgtacgc acgtattata
    13081 tgtataaatt gtgtaacata gaaggaaatc cgtcttgcaa aaagaatgcg tatgatattg
    13141 agcgattgct taagcaaaaa acgatgaaaa acattttaaa gaatgtgaaa atagcagaaa
    13201 gcttagatga tatactatga gcaaaactaa atatttaatt aaaaatatgg gaatattaac
    13261 aataagtaat ttttcttcaa aaattttagt ttttttgtta gtccctttat atacaagtgt
    13321 attatccaca acagaatatg gcacatatga tttgacaatt tcaacagcca cattattgtt
    13381 tccaatttta accttaaaca ttgtagatgc tgtgatgcgc tttttaatgg ataaagataa
    13441 aaataaaaaa aatatagcat ctatagcgat taaagttata tttttaagcg tgatagtctt
    13501 tggatccgta atgtatttgt taaatattac aaaattatgg caagatg
//
LOCUS       DANKMN010000080        13090 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000080 DANKMN010000000
VERSION     DANKMN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..537
                     /locus_tag="PW224_002375"
     CDS             <1..537
                     /locus_tag="PW224_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2909029.1"
                     /translation="HDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHG
                     SQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGP
                     DLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSD
                     KLLNRIDKEILKVGNIDE"
     gene            530..1957
                     /locus_tag="PW224_002376"
     CDS             530..1957
                     /locus_tag="PW224_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL2909030.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1959..3029
                     /locus_tag="PW224_002377"
     CDS             1959..3029
                     /locus_tag="PW224_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909031.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            3016..4437
                     /locus_tag="PW224_002378"
     CDS             3016..4437
                     /locus_tag="PW224_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2909032.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            4450..5457
                     /locus_tag="PW224_002379"
     CDS             4450..5457
                     /locus_tag="PW224_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2909033.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDAIELMSGQEFIRIAKENNVVT
                     W"
     gene            5469..6473
                     /gene="neuB"
                     /locus_tag="PW224_002380"
     CDS             5469..6473
                     /gene="neuB"
                     /locus_tag="PW224_002380"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL2909034.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            6470..7618
                     /gene="neuC"
                     /locus_tag="PW224_002381"
     CDS             6470..7618
                     /gene="neuC"
                     /locus_tag="PW224_002381"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL2909035.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            7591..8268
                     /locus_tag="PW224_002382"
     CDS             7591..8268
                     /locus_tag="PW224_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2909036.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            8258..9400
                     /locus_tag="PW224_002383"
     CDS             8258..9400
                     /locus_tag="PW224_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2909037.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            9413..10390
                     /locus_tag="PW224_002384"
     CDS             9413..10390
                     /locus_tag="PW224_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2909038.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            10390..11298
                     /locus_tag="PW224_002385"
     CDS             10390..11298
                     /locus_tag="PW224_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HDL2909039.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            11299..12051
                     /locus_tag="PW224_002386"
     CDS             11299..12051
                     /locus_tag="PW224_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2909040.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            12056..13003
                     /locus_tag="PW224_002387"
     CDS             12056..13003
                     /locus_tag="PW224_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2909041.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         4706 a         1633 c         2354 g         4397 t
ORIGIN      
        1 catgattatc ctagaattaa taggaataac acaactgcat ttaaaatttt tagagctata
       61 attaatcgtt gtctgaggat gattaaactt cccccaaaaa ttagacacca taaatttgat
      121 ttgtatcatg gttctcaatg gtgggctttg tcgaaagaat gcctaacaga attaatacaa
      181 atgtatgaac aaaatcaaca agactacctg aattttaaaa taggtatgtt tgccccagat
      241 gaaaaatttt ttcatacact tttttttaat tcctcgttca aaaataaaaa tgttattgga
      301 ggaccagatt taccacttga attgaaaaat attgaagaaa ctactttaca aacttcgaaa
      361 ttagcaaata ttcatattat tgatccttcc atgaacaaat ggtttgatga aagggatata
      421 gatcaaataa aagaaagtga aaaattattt gtaagaaaag ttaaatcgga ttactctgat
      481 aaactattaa atagaattga taaagaaatt ttgaaagttg gaaatataga tgaatagaat
      541 agtacctgtt ttagagaaga gtttattaag tcgagatgcg tttcgagtaa taaatttaat
      601 catttgtctg ctttatttta tgcctagtac ttttaatgta actaatatcc cgatgaaaat
      661 agcttttgct tggggtggaa ttatagtttt gtatgaggtt tttataaaga aaaatttttt
      721 taaaatgcgt ttttcttggc tactgattgg agcaatgatt agttttatct taactataat
      781 cattaacaga gaaaattttt taattccatc tatttataat tttggatatt tgctgattac
      841 tttgatagta atttttcctg cagacttttc acaaagtgaa gaggaaaaaa agaaaaaaat
      901 ggtgtatttt aataatgttt tcattatcat aattttttta gctgcgctaa tatctatcta
      961 tcaatttatt tttttgattt cttatcatgt cccaacagga acgccaggat tacttgcaag
     1021 acaaggtttt attgaacatc ggttatttgg tgtctataca agtccaaatg ttggagctat
     1081 gtttggatat acttctatta ttttaagttt aattagtgta ttaatttcaa aaataacaaa
     1141 aacgattcgt gttttttata tgatgaattt tatcgtacaa atattttata ttacactttc
     1201 ttcgtctaga ggtgcccagg tagtaatttg ttctttttta gtattatttc ttttgttgtt
     1261 tgccaattca aaatttagaa agagcattag agaagtacta cctgtaaaaa tcaaatggat
     1321 ggtaagcata ttattagttc tattggtata tttttctttt ggtactaatt atgctaagga
     1381 ttttttagca acaattcctg tatcgattga tagaaaagta gaagttatag agtcaaatga
     1441 atcagataaa actccacttc cagaaaaaga agtaatatta cagcattcta gtgataactc
     1501 cgaaatttca gctggtcgtt ttacgatttg gaaagctgcg cttaaagtta taagaccaac
     1561 tttgttattt ggttatggag agaccgattt ctatgggaat agcgatgcaa aatttttgaa
     1621 aaaagtagat cttgatacta cagatgtgaa tgaactaaaa agagcacatg gaaacatgca
     1681 taatggattt cttcaagtac ttgtatcaag tggtataatt gcttttatat gtatctatgc
     1741 tttctatgtg ttaaatatct ttagtttact caaaacattt tttagaagta aagtgaatta
     1801 tgcgtttctt ggtattgttt taatttttat actatctatt tttatagatg aaatggtgga
     1861 ggctcatgta cttttcaata agcgcgatgt catttcaatt gttttctggt attatttggg
     1921 aagcatttct tacttgtatt taaaaaagga gaattaatat gaagacagct ttcattgctt
     1981 ggactccttt acatttgatt aatatattaa atgtttatca aaatttttac cgagaatcaa
     2041 aaggagattt atttatttat tctgaatttg aaaacgctag aagatatagt gagcgtataa
     2101 aagaattagg gctatttaat gatgttgttt ttgtgaatca ccggaaaatc ggttcttcct
     2161 ttaaacgtaa aattaacttg ttaactaacc ataatcaatt tttctcggat gatttatccg
     2221 tttatgatga aatttttata cagggggaaa attatttcac aaaaatactt ttctccaatg
     2281 tttacaaaaa aaatccagat gtaaaactaa attatataga agatggtttg ggtacttatt
     2341 tgaagaagag tatattttca acaagtaaaa acgttcaaag agtttttaaa atattaaata
     2401 aaaatagcat cttcaaaaaa aaaattgacg attattttgt ctattgtccg gaattgttac
     2461 tttatgatgt aaaaaatata aaaaaaattc ccaaaataga tgaaaacagt aaattgatga
     2521 aaaacataca atcaatcttt gggatagaag aagtttttgt cggagataaa tgtatctttt
     2581 ttgatcaacc tttattatct gatggttatg gcatagatga aacagatatt ttcgctaaag
     2641 taaatagtat attttctgga aaaaaagaaa ctttaattaa gcttcatcct agagcaagga
     2701 caaatcgtta tggtgaaaaa aacatattga attccagttt accgtttgaa gtcttgctac
     2761 ttgaaaatga cttctcttca acacttttga ttagtcctat atctacaatt tctttttcac
     2821 catctatgat gtttgaaaag acgatcaact ctattatgtt ggctaaatta attttaaatg
     2881 aatatacagc attatctgaa gataaaaaag aaacattgac aaatattgtt cttttctgtg
     2941 aacattataa ccgtctatca gaagaaaaaa ttttactccc agaaaactgg gcagaattaa
     3001 gaaagttggt agagtatggg attatttaac acactttcca agtcaaaatt agttaagtcc
     3061 agtttctggt atactatagg gaattttttt attaagggaa tttcattcat aactattcct
     3121 atttttgtaa atattactac tcaagcagaa tatggtttga ttaataattt cacttcattt
     3181 gtatcaattt tttcgattct tgttgggcta agcctaaatt cggcaattaa taatgcaagt
     3241 tttgactata aagatgacat aaatgaattt atgtcatcgg tactttctct agctacactt
     3301 tcgttaattg tgttcttatt aatagggaac atatattttt tgttttcaaa tgcttttttt
     3361 gatatgaacc aacttgtatt taatttgatg ttattacaaa gttttgctag ttttgtaatt
     3421 agttatatcc agtcttattt tacgattaac tttaaccatt ttaaatattt gattttatct
     3481 tttatatcta cttttttgaa tattgcaatc tctgtagctt tgatgcagac attatatttt
     3541 gatgataaat atgttggcag agtagtcggc ggaacactgt catttgtgtt gataggttta
     3601 gtaatttatt taagaataat gataaagggt gctcattttt ttgataaaaa acagtggaga
     3661 tacgctttaa aaatctcttt gccgctaatc ccacattcct tagcaaatat agttttagca
     3721 caattcgata gaattatgat taattcttat ttaggtcctg ctgcgggggg aatatattcc
     3781 tacattagta acataggtat tattctaagc gtaatatgga tgtctactaa taacgcctgg
     3841 gttccatggt tctatggtga gatgagtaaa aataatgaaa aaaaagtgaa gaaagttagt
     3901 aactattatt tagtagtctt tactttgatt acgatgattg taatggtagt ttcgattgat
     3961 ttagcaaaat ttatggctcc gaaagactat tattctgggt tatctttagt ggttccgata
     4021 accttaggct atttctttca gtttttatat agtttaccag ttaatgctga attttatgag
     4081 aaaaaaacat cgtttattgc gattggtaca gtagcaagtg ctatagttaa tatactatta
     4141 aacatgattt tcatacctag gttaggaata attgctgctg gatacacaac cgtatttacg
     4201 tattttttac tttttatttt tcattataat atcgctaaaa aaattacgaa taaacaatta
     4261 tttgacacaa aaaaaatatg tatcataata atttttgtat caattttttc tttgatactt
     4321 catattttaa tcaatcaaat gttgattcgt tacgttttga catcattatt gataattagt
     4381 ttagttatat attttttaaa gattaagaat cgaattatag atgactataa ttcataggag
     4441 gagatataag tgtttaaagg aaaaacaata ttagtaactg gcggaactgg ttccattgga
     4501 agtgaaattg ttcgtcagct tttaaaatat gaaccaaaag ctatcagaat ttatagtaga
     4561 ggtgaagaaa aacaatttta tatgcaacaa gagctctcaa atcacactaa tttgagattt
     4621 ttaattggtg atatcagaga ctctcaaagg attgattatg catgtcgtga tgtggatatt
     4681 atttttcatg ctgccgcaat gaaacacgtt ccagcaagtg aatataatcc tatggaagct
     4741 gtaaaaacaa atgtgatggg aactcaaaat gtaatagatg cagcgatcaa aaataatgta
     4801 aaacattttg taggtatcag tacagataaa gtagtgagtc ctactaacac catgggtgcg
     4861 acaaagttat tgagtgaaaa attaataata gctgccaatc agtataaagg aaaaacagaa
     4921 acaatatttt cttgtgtaag gtttggaaat gtcatggggt caagtggatc tgttattcct
     4981 ctttttagta aacaaataaa aaacaataaa gatattactt tgacagatgt tgatatgaca
     5041 cgatttatga tgtcaatacc ggatgctgca agattagttc tgtctgctgc tatgaattca
     5101 aaatttggag aaacatatgt cttaaaaatg ccggcaatca atattttaac tttagctcaa
     5161 tcattaataa aaatatataa tgatacacat gatgaaaaat atagaggtga aataaaagtt
     5221 gtaggtatca ggccaggaga aaaattatat gaagagctaa tgactacaga cgaaattgaa
     5281 cgctcatatg aaaatgataa aatgtatatc attccaccac actatcatgc tactgacgaa
     5341 ctgtacaaag attatgaaaa aattaaaatc actgcatact cttcagatgc aatagaatta
     5401 atgagtggac aggaatttat tagaatcgca aaagagaata atgtcgtgac ttggtaagga
     5461 gttttatgat gaaatcagaa gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa
     5521 gtctagattt agctttagaa agtattgata aagctaaaga atgtggagct aacgcggtta
     5581 aatttcaaac atttaaaacg aataagctag tttccaaaaa ggctccaatg gcaaaatatc
     5641 agaaggataa tcttaaagag gacagttctc aatttgaaat gctaaaaaaa ttagaattat
     5701 ctcaatctga ttttattaga ataaaagatt attgtataca aaataaaatt gattttttgt
     5761 ctactccttt tgatgaagaa agtgcagact ttctaaaaca aattggtgta caaggattta
     5821 aaattggctc aggagattta acaaatttac cttttcttag aaaaatagat atgtatgggt
     5881 tgccagtttt attatctaca ggtatggctg atattcatga ggtcagtgag gctgtagatt
     5941 gtttcataga tagtccagtt actattttac attgtacttc aaattatccg gctagttctg
     6001 aagatattaa tttattggcg attaacacat taaaagaaat gttcgggata cccgttgggt
     6061 actccgatca ttcattggga tatgatgttg gtatttgtgc tgttgcgatg ggtgctaagg
     6121 taattgagaa acattttacc ttggataagg aactacctgg tccagatcat aaagcttctt
     6181 tagatcctca ggaatttgca gattttgtaa agcatattag aaacactgaa atatttttag
     6241 gtgatggtgt taagcgagca atgccttctg aacttagtac aaaagaagta tcaagaaaaa
     6301 gcctagtggt cactgagaat ttaaatgtgg gagatattct tacagagaaa tctcttacaa
     6361 taaaacgtcc tggaaatgga atagctccta agtttttata tcaatatatt ggcaaaaaag
     6421 ttaaaagacc aataagtgca gatagtgttc tgcttgagga agatgttgaa tgaaaaaaat
     6481 acttatagta agtggaacac gagctgatta tggaatatat aaacctgttg ctgagaattt
     6541 actacaagat aattgggatg tatcatttgt tgtttctgga atgcatttat cccataaata
     6601 tggatttact aaagaattga tcttagctga cggatttaga attagtggag aagttcaaag
     6661 tttgttttta gagaacaata agggaaacat ggctcggtct gtggcattag agataaatgg
     6721 tttcacaaat atatttgaac aaactagccc tgattttgta ttattactag gggatagagg
     6781 agaaatgttg gcagctgcga tagcttgttt gtatcttgga atcaaaacag cacatattca
     6841 cggaggcgaa gtgtctggta ctgtagatga atcaataaga catgccataa cgaaaatagc
     6901 atctgttcat tttgctgcta cggaaaaaag caaagagcgt ttaataaaaa tgggggaaga
     6961 ttcttggaga gtttttcccg tcggagctcc tagaatagat actattttaa ataatagatt
     7021 acctagtttt agagatataa aagaaaaata tcatctaaaa tttgaaaaga aaaaatacgt
     7081 tttgcaagta tttcatccag ttgtaaccga actagaaaat atagaaaaac aaattttagc
     7141 tttgatcaat tcaatggaac aacaatctca accaattatt tgcattttac ctaatagtga
     7201 tgctggtagc gaaattatca gaaaaatgta ccagaaatat ttaaaaacgg atattattta
     7261 tattacgaat ttagagccgg aggaatacct gactatatta aaagagtgca aatttatgat
     7321 cggaaattct tcttctggga taattgaagc tgcatcattc aaaataccag taataaattt
     7381 aggaaccaga caaaatggac gagaacaatc taaaaatatt ataaatgcaa atctaaatga
     7441 aaatgaaatt gaaactgctt tgaataaaat acaagatcct cagtttataa aaagcttgaa
     7501 aaatgttaag aacatctacg gtgacggcaa cagttctaga agaataacaa aaacattgaa
     7561 tcaaatattg aatgatgggg aagatattaa gtggattcaa aaacaaatag cttattaaaa
     7621 tatactagtg atccatacat tattattggg gctggcggac acgcaaaagt cattattgaa
     7681 accttaagat tagaaaataa gttaatatat ggattttttg atgataagca aaataaaatg
     7741 caagatctga aatatcttgg gaaaataaac gaattgaata gctgggcgga tagcaaaaaa
     7801 aaaatctata aatttattgt agctattggt gactgtgtag taagaaaaaa gattataaaa
     7861 aaattgaata tgccaagtga gcaatatgga atagctgttc atccaagtgc tatagtggca
     7921 aaagaaattt caattggtta tggaactgtt atttttgcga atgctgtaat taacccagat
     7981 gctgtaattg gagagcatgc tataataaac acaggtagca ttgttgagca tgattgtaga
     8041 attaataact atgttcattt atctccaggt gtatgcttag cgggtggagt acatgtaggt
     8101 gtaggaactc aaataggaat tggttctcaa tgtatccaga tgaagaaaat tggatcttgg
     8161 tgtatgatag gcgcagggag cactattgtt aaggatattc cctcacattc cttagcttat
     8221 ggcaatccag ctaaaattaa gaaagaagga attaattttg aattctagaa ttttattatc
     8281 aagtcctcat atgggaggaa cagaagaaaa atatgttgct aaggctttta aagataattg
     8341 gatagcgcca ttaggtgaaa atgtggataa atttgaagaa agtattacaa aatatactgg
     8401 ttctaagggc ggagcttctg cgttaagttc aggtacagct gccatacatc ttgccttaag
     8461 actgcttaat gttggaaaaa acgatattgt ctttgctcct tcatttactt ttatagctac
     8521 agtaaatcct attttatatc aaggggctgt accagtgttc attgactcag aacctaatac
     8581 atggaatatg agccctgttg cactagagag cgcattagaa gaagctgaaa gaaacaacca
     8641 attacctaag gcaatcatcg tagtgaattt atatggtcaa atggctgatt atgaacgaat
     8701 tttagctatt gcaaaccaat ttaatgttcc agtgatagaa gatgcagcgg aatcgttagg
     8761 ttctacttat aagggaattt cgtcaggctt atttgggaca attgggatct attcatttaa
     8821 cggtaataag atcatcacaa cctcaggtgg aggagctcta gtgtctagtg ataaaaaata
     8881 tgttgagcat tcaagatttt tagcaacaca agcaagagat caagcaccgt attatcaaca
     8941 tagcgaaata ggttataatt accgtctaag caatatttct gccggtatcg gacgtggaca
     9001 aatggaagta ttgaatgata gagtagctaa acgaagagaa attttcaatc gatataagga
     9061 agctttttct ggaattgata gaattgaaat gatgccagaa gtggatggca tggtgccaaa
     9121 tcgttggttg tctactttaa cgatcaatga ttataattct attgatcaag tagttaacgt
     9181 aataaataaa ctaaatgaaa aaaacattga aacaagagct ttgtggaaac cactacattt
     9241 gcaaccagta tttcaagggg taaaaatgta ttaccatgat aataatctta gtgtgtcaga
     9301 aaagttattt aatagcggct tatgcttacc ttctggaagt aatatgacag atagtgaaca
     9361 agaatttgtt attgatgagt ttaaaaaaat attcaagtag gtgtgatatt ttatgaacat
     9421 aggaattatt ggtttaggtt ccatggggaa aagaagactt agactcattc aagaacactt
     9481 taatgatatt ttactatgtg gtgttgatac taatgaggcg agacgaaagg aagttgaaaa
     9541 ggaatttgga ataccaactt ataatagtct aacagaggca aaaaatttgt ttgaaataaa
     9601 ttcagttttt gtttgtacat cccctataac ccatgaattt attataaaag aggctttaaa
     9661 tttagattgt aatatcttta cggaaatcaa tttattaaat gaatattatg atgaaataat
     9721 cgaagtagct aaaaagaaag atagattact ttatctatct tctacatttt tgaagagaaa
     9781 agaaattcaa tatattaagt ctaaagtgac gcataataaa aaaatttctt atcaatacca
     9841 tgtaggacag tatttacccg actggcatcc ttgggaagat tatcgcaatt tttttgtttc
     9901 aaacaaaaaa actaatgggt gtagagaaat attcgctatt gaattacctt ggattataga
     9961 tacatttggg aaagttaaat cgtttactgt aatgacccag aaattatcca atcttgatat
    10021 tgattattcg gacagtttca ctcttattat tgagcatgaa actggtatag ttggaacttt
    10081 aagtgtagat gttgtttcaa gagtagctaa aagaaattta actataattg gtgaaaagat
    10141 tttaatagaa tgggaaggta acccggattc tctattttca tggaatgaag aaacttctag
    10201 tatgacagat attcaattgt acgatgaggt tatgcatgat aataattatt cacatactat
    10261 tattgaagat gcatatttag aggagattaa agaatttttc acgcttttgg aaaagaaaat
    10321 ggaaccccaa tatacttttg aaaaagataa gtatattatt aacttaatta acgacattga
    10381 ggtaaattaa tgatgaaagt aatttttttt gggttagggt caattggaaa gagacatcta
    10441 aaaaatctaa taagctatgg aaaaaagaat tctattaatt ttgatataac agcttatagt
    10501 agcagtggaa aaaaagagtt tgaagaaata acgtatattc aaaatagaga cgatttggat
    10561 acacattatg atatagcttt tattacaaat ccgactttta agcatctgga aacactaaat
    10621 gtaattgttg aaaaagttga ttacatattt ttagaaaaac ctgctttttc tgagagtatt
    10681 gatcttactc aatataatta tgattacaaa aatatttatg ttgctgctcc actgaggtat
    10741 aaaaatatca tggaagttgt tcgtaatagt attcaaagta aaaaagtata ttctgtaaga
    10801 gctatttgtt ctacttatct tcctgattgg agaaaaggta ctgattatag aaataattat
    10861 tctgccttta aatctatggg gggaggagta gaattagact gtatacatga attggattat
    10921 attataaacc tatttggttt ccctaaatca gtaaaaaaac agtttggtaa atattcgaat
    10981 cttgaaatta acagtaatga tttagcaaca tatatattga gatacgatga taaaattata
    11041 gaaattcatt tagattattt tggaagagtt cctacaagaa aatttcaatt gtttacttta
    11101 gatgaatgta tcaatgttga cttattaaat aataaaataa gttttgaaga aaacattgtt
    11161 aaagaaatgc ctgaatctgc taatgatatg tatgagaagg agttgaaata tttctttgaa
    11221 aacgtagtaa caggcaaaga aaactggaat gatttatatc atgcaaacga agtattgaaa
    11281 atagctgagg agaaataaat ggaaatttta tttactattt gtggtagagc tgggtctaag
    11341 gggtttaaaa ataaaaattt gaaatatttg gcaggaaagc ccctagtcca tttcgcttta
    11401 gcaatgatag atatctatca acaaaaacat cctgaacata attgcacgac ttgtttgaat
    11461 acggacagta atgagttagt agaattgacg actaagttaa atccgtctgt gaaaattgta
    11521 gaacgtaaac ctgaattagc tacagatact gtagggaaaa ttgaagtgat acgtgattca
    11581 ttcttacaac tagaaagaga agataatata tttgatttaa ttatagattt agatttgact
    11641 tcacctttac gtgaagtttc taatcttgaa gaattaatag atgaacatct aaaaaattta
    11701 gataaagacg tctacttttc agttgtttct gcaagacgta atccctattt taatatggta
    11761 aaaagaattg aaaatgaagt tactatagtt aataaatcaa gatacacatc taggcaacag
    11821 acacctgaag tttttgaaat gaatgcctca atgtattctt ttaaacctga atttctaaaa
    11881 aaaagtaatt atatttttga tgggcaatgt gggataataa aaatgagaga ttatctaatt
    11941 ttagatattg atagtgaaga agattttaaa tggttagaat ttttgtttcc taaattttta
    12001 gaagattctt cggagcttca agatatctat aattatttat atcggaggta aaaggatgag
    12061 ctgttatctt atcaccggtg gggctggctt cattggttct accttagcca atcatttagg
    12121 agaagaacat gtggtaatcg tcgtcgatga cttatcaatg ggaaaagtag aaaatcttga
    12181 tatgacgaaa aatatcactt ttattgaggg agatgtcgct gagaaatcct tgatggaaga
    12241 gatcatgaaa gctaataaat ttgattatat ctttcattta gcagcagttg ctagtgtggc
    12301 agattcagtt gcccgaccag tagaaacaca tcgtgtgaat tttgaaagtg tattaatgat
    12361 tttagaattg atccgcaagt atcaacctga tttaaaacgg atcgtgttta gttcttcagc
    12421 ggctgtttat ggggatgaac cgactttacc aaaaaaagaa gagtccgtga ttcgcccatt
    12481 gacgccttat gcggttgata aattcgctgc agaacaatat gtgctagatt attgtcattt
    12541 atatgatgta cccggtagcg cagttcgttt tttcaatgtc tatggtccaa atcaaaatcc
    12601 gaattctcca tacagtggtg tgatttctat tctcgtagat cgctataaga aacaaatcgt
    12661 aggagaagca acttcattta cattatttgg tgatggaagc cagtctagag actttgttta
    12721 tatagatgat gtaatccaag cattactttt agttgcgaat gaggaagctg cactggggaa
    12781 acaattcaat gttggaacgg gtaaagcaac cacacttttg gaattgattc atgcaatcaa
    12841 tgaaattctc ggtgtagaat tgacacttga atacaaagaa gagcgttcgg gtgatatcca
    12901 tgactctcta gcagatatta ctaagatcca gtcaattggc tatcaaccga aatatggtat
    12961 tttaagtgga atgactaatt atttgaaaac tgaaatcaat taaaacatac taagataaga
    13021 ttagagggta aattcaggct ctttgtcaat aaggactgat gagttttgta aaatcaaatc
    13081 tgggatagaa
//
LOCUS       DANKMN010000081        13025 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000081 DANKMN010000000
VERSION     DANKMN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            410..979
                     /locus_tag="PW224_002388"
     CDS             410..979
                     /locus_tag="PW224_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL2909042.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            966..1826
                     /locus_tag="PW224_002389"
     CDS             966..1826
                     /locus_tag="PW224_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2909043.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2016..2720
                     /locus_tag="PW224_002390"
     CDS             2016..2720
                     /locus_tag="PW224_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2909044.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2725..4560
                     /gene="walK"
                     /locus_tag="PW224_002391"
     CDS             2725..4560
                     /gene="walK"
                     /locus_tag="PW224_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL2909045.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4557..5873
                     /locus_tag="PW224_002392"
     CDS             4557..5873
                     /locus_tag="PW224_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909046.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5874..6743
                     /locus_tag="PW224_002393"
     CDS             5874..6743
                     /locus_tag="PW224_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL2909047.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6798..7607
                     /locus_tag="PW224_002394"
     CDS             6798..7607
                     /locus_tag="PW224_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2909048.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7710..8339)
                     /locus_tag="PW224_002395"
     CDS             complement(7710..8339)
                     /locus_tag="PW224_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL2909049.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8509..9801
                     /locus_tag="PW224_002396"
     CDS             8509..9801
                     /locus_tag="PW224_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2909050.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9877..10539)
                     /locus_tag="PW224_002397"
     CDS             complement(9877..10539)
                     /locus_tag="PW224_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2909051.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10764..11048
                     /gene="groES"
                     /locus_tag="PW224_002398"
     CDS             10764..11048
                     /gene="groES"
                     /locus_tag="PW224_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL2909052.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11099..12724
                     /gene="groL"
                     /locus_tag="PW224_002399"
     CDS             11099..12724
                     /gene="groL"
                     /locus_tag="PW224_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL2909053.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4337 a         2234 c         2803 g         3651 t
ORIGIN      
        1 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
       61 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      121 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      181 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      241 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      301 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      361 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      421 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      481 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      541 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      601 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      661 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      721 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      781 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      841 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
      901 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
      961 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1021 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1081 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1141 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1201 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1261 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1321 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1381 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1441 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1501 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1561 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1621 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1681 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1741 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1801 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1861 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     1921 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     1981 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2041 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2101 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2161 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2221 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2281 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2341 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2401 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2461 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2521 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2581 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2641 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2701 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2761 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2821 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     2881 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     2941 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3001 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3061 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3121 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3181 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3241 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3301 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3361 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3421 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3481 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3541 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3601 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3661 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3721 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3781 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3841 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     3901 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     3961 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4021 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4081 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4141 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4201 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4261 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4321 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4381 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4441 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4501 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4561 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4621 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4681 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4741 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4801 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4861 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     4921 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     4981 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5041 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5101 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5161 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5221 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5281 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5341 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5401 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5461 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5521 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5581 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5641 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5701 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5761 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5821 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     5881 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     5941 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6001 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6061 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6121 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6181 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6241 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6301 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6361 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6421 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6481 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6541 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6601 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6661 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6721 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6781 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6841 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     6901 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     6961 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7021 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7081 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7141 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7201 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7261 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7321 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7381 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7441 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7501 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7561 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7621 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7681 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7741 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7801 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7861 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     7921 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     7981 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8041 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8101 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8161 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8221 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8281 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8341 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8401 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8461 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8521 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8581 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8641 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8701 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8761 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8821 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     8881 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     8941 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9001 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9061 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9121 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9181 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9241 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9301 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9361 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9421 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9481 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9541 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9601 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9661 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9721 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9781 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9841 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
     9901 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
     9961 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10021 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10081 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10141 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10201 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10261 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10321 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10381 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10441 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10501 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10561 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10621 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10681 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10741 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10801 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10861 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    10921 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    10981 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11041 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11101 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11161 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11221 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11281 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11341 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11401 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11461 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11521 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11581 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11641 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11701 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11761 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11821 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    11881 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    11941 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12001 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12061 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12121 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12181 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12241 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12301 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12361 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12421 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12481 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12541 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12601 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12661 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12721 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12781 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12841 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    12901 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    12961 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13021 ccatt
//
LOCUS       DANKMN010000082        12911 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000082 DANKMN010000000
VERSION     DANKMN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(24..959)
                     /gene="murB"
                     /locus_tag="PW224_002400"
     CDS             complement(24..959)
                     /gene="murB"
                     /locus_tag="PW224_002400"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL2909054.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1032..1952)
                     /locus_tag="PW224_002401"
     CDS             complement(1032..1952)
                     /locus_tag="PW224_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2909055.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2065..2817
                     /gene="xth"
                     /locus_tag="PW224_002402"
     CDS             2065..2817
                     /gene="xth"
                     /locus_tag="PW224_002402"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL2909056.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3681..4214
                     /locus_tag="PW224_002403"
     CDS             3681..4214
                     /locus_tag="PW224_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL2909057.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4704..5228)
                     /locus_tag="PW224_002404"
     CDS             complement(4704..5228)
                     /locus_tag="PW224_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2909058.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5221..5694)
                     /gene="tsaE"
                     /locus_tag="PW224_002405"
     CDS             complement(5221..5694)
                     /gene="tsaE"
                     /locus_tag="PW224_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL2909059.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5817..6797)
                     /gene="pta"
                     /locus_tag="PW224_002406"
     CDS             complement(5817..6797)
                     /gene="pta"
                     /locus_tag="PW224_002406"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL2909060.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6950..7630)
                     /locus_tag="PW224_002407"
     CDS             complement(6950..7630)
                     /locus_tag="PW224_002407"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2909061.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7756..8625
                     /locus_tag="PW224_002408"
     CDS             7756..8625
                     /locus_tag="PW224_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2909062.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8651..9166
                     /locus_tag="PW224_002409"
     CDS             8651..9166
                     /locus_tag="PW224_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909063.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9213..9806)
                     /locus_tag="PW224_002410"
     CDS             complement(9213..9806)
                     /locus_tag="PW224_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2909064.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9831..10010)
                     /locus_tag="PW224_002411"
     CDS             complement(9831..10010)
                     /locus_tag="PW224_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909065.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10087..10596)
                     /locus_tag="PW224_002412"
     CDS             complement(10087..10596)
                     /locus_tag="PW224_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909066.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10692..11072)
                     /locus_tag="PW224_002413"
     CDS             complement(10692..11072)
                     /locus_tag="PW224_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2909067.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11402..12151
                     /locus_tag="PW224_002414"
     CDS             11402..12151
                     /locus_tag="PW224_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL2909068.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12472..>12911)
                     /locus_tag="PW224_002415"
     CDS             complement(12472..>12911)
                     /locus_tag="PW224_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2909069.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         3732 a         2612 c         2321 g         4246 t
ORIGIN      
        1 cttattttaa gttccattac ttattaattg gctaaacttc tgcttgtttc tgatattgct
       61 tgtttttctc caatgatccg cacttctgtt tctaaagaca catcaaattt ttctttgatt
      121 acttcttgga tgtgcgcaat caattcaata taatctgtcg ctgtcgcatg atcgatattg
      181 acgatgaatc cagcatgttt ttcagagact tgtgcaccgc cccatttcag gccttgcaat
      241 cctgcttgtt ggatcagctg acctgtatag tgtcctactg ggcgcttaaa gacacttccg
      301 catgaagggt attctaatgg ctgttttgat tgacgcaatt cggtcaactc atccattctt
      361 gctttgattg cttcataatc ccctgtttgc aaactgaatc gagcttccaa tacgatagca
      421 cgtaattctt ggactttact gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt
      481 ttcaatgttc catcttcaag caaaagatcc acttcagcaa aaacatcttt gatctctccg
      541 ccatacgcac ctgcattcat ataaaccgct ccaccgacac ttcccgggat gccgcaagca
      601 aattcaaatc cagtcaatga ttctgccaaa gctgcataag tcgtatcaat cagtgaggca
      661 cctgcttccg caatgaccat cgttcctttg acatgaatct gtttcatttc agtcagcatg
      721 atgactacac cgcgaatccc gccatcttga acgatcagat tgcttgcatt tcctaatacc
      781 atccaaggta tatcatggat tcggcaataa tcgatgattt gctttacttc ttcttgtttt
      841 ttcgggaatg caaggacatc agctggtcca cctgttttcg taaacgtata gttcatcaat
      901 ggttcatcaa acaatatagt aatcgtggct aagctttcaa ctatttcttc tttattcact
      961 gaggattttc ctttcttttc tcaaacgtcc tcattattct atcacgctta atacaatctt
     1021 gccagtcttg ttcaaagttg atgacggtag tgatctttaa gcacttcctc acataattca
     1081 tgacttctta caacttcttt ttgtttaagc aattctgttt ccgatttacc taaacggatc
     1141 tgagaaaaga aggcctctac cattggctca aacccgcgtt tgactagcgt gtttgtccag
     1201 tcgccaaatt ctttcacttt ttttccatca gcatcttgaa tgacaagctg ggtcagatcg
     1261 tttacttcat aagtaccatt tttacttgtt acttgatatt tttctatatt agcgccactt
     1321 gctaaatcca tcgttaaaat agccgtttga ttttgtgttt ctaattgtaa aatggcatat
     1381 tccaaaaatt cctttgtttc tcttatctgg tattttgttc tcaaaatggg ttcatctaat
     1441 aaatacacag ctgtatcaat aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt
     1501 tcttttgccg ctattcgatt tttttgcagg tggatcaacc gcttatctgg gatacgtttc
     1561 aattcttcta ccataggagc aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc
     1621 ttatcagcta attcttgcaa ttcacgaact tcatttagat ggacactcag tggtttgtca
     1681 atataaacat ggataccatt ttctagacat tttttggcta attcataatg tacttttgtt
     1741 gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa gctcatcgat cgtttcatat
     1801 agatgttgga acccgtactc tttttgaatt ctttcttttg tttctttatt tcttgttgca
     1861 aaataaaaat caagtgttcc ttgttttttc gcataagttg gtaaatatgc tttttctgcg
     1921 atattcccta caccgattac accaatcttc atctactttc ctcctggatc atttagttac
     1981 tttcatctta caccattttt tactaaaatg aggtacaata gtatcgcaaa ggtaaggaaa
     2041 ttaattcgaa ggagtgtgct tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt
     2101 gccatcgtaa ataaaaattt tttagaagtt tttcatgagt tagacgccga ttttttctgt
     2161 ctgcaggaaa cgaaactcca agcaggacaa attgatttag atcttccagg ttattatcaa
     2221 tattggaatt acgcggaacg aaaaggctat tccggcacag cgatttttgc taaaaaacca
     2281 gcattgaatg ccacttacgg aatgggtatc gatatccatg atacagaagg ccgtctgatt
     2341 actttggaat attccgattt ctttcttgta acctgttaca cgccaaattc tcaaagcgaa
     2401 ttaaaacgtt tagattaccg attagaatgg gaagaagcct tttataatta tttggaaaat
     2461 ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt
     2521 gatttgaaaa actggaaaac gaatcaaaaa aatgctggat tcactccaga agaaagagcg
     2581 gctctttctc gactgttgga taacgggttt atcgatacat tccgttattt ttatccaacc
     2641 caggaaggtg tctactcttg gtggaactac cgattcaatt caaggaaaaa caatgctggt
     2701 tggcggatcg attacttttt gacgagcaaa gatttagagc caggactagc tgatgcaaag
     2761 atccatacgg acattatggg cagtgatcat tgtccagtag aattagattt gaaataaaga
     2821 ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
     2881 acattttgca taaatttggc ataataccga gaaactggct tttgaacact gtttatcgag
     2941 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
     3001 catatttttc gtttggttgg gcttattacc gcagatacaa gcttttgaac accgtttatt
     3061 aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac aaaaatagtt
     3121 cctcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
     3181 attaagaggt tgtgaaaaag ctgcactgca tcaagtaata agaacagcca aacaaaaata
     3241 gttcctcata tttttcgttt ggttggactt attaccgcag atgcaagctt ttgaacaccg
     3301 tttattgaga agttgtgaaa tcagcgtcct gacctgagta ttagagaaac aaatggcaaa
     3361 atagttcctc atattttgct attttttgag ctaataccga gggtcagctg attgaacacc
     3421 gtttagtgga gaagttgtga aaaagtcgcc tactttcgag tcgtaagaaa tttttttgaa
     3481 aatggttcct cacaactttg cataaatttg acttaatacc gagaagtcat gatccccatc
     3541 taactccaaa tcacaaggaa tgagttcaaa aactagcttt tctcaacaaa tattacataa
     3601 aaaattctat gttttttgtt aaatttattt gcttttttca aaagtatgtt acagtaattt
     3661 cccaaggagg ttttacagaa atgaattttg ttgccatgga ttttgaaact gccaatcatc
     3721 agccttatag tgcttgttct cttgcgctag tcatggtaaa aaatagccag atcgttgatg
     3781 aattctatac attgatacag cctgagactc cttttttctg gcgaaatgtc caaattcacg
     3841 gaatccatca agaagacgta cgcaatgcgc cgaaattccc agaagtctgg caaactatcc
     3901 aaccatattt tcaaaaaaat cgtttagtcg ttgcacataa tgctgctttt gatacaaagg
     3961 tcttagcggg atgcttagat tattatcatc ttgaacagcc taattatctt tctttatgta
     4021 cagtgaaaag tagccgtcgc ctgtttcctg aatttccaaa tcatcgattg aacactgtgt
     4081 gcgaaaattt gaacatccaa ttagatcatc accatgatgc attggaagat agtcgtgcat
     4141 gtgcggaaat cctgctttat caagaaaaaa aattcgggac agatccattg aaaaaacttg
     4201 tgactataaa gtaaaagaat acttatgaaa cacacaaaga ggtcgtgagc ctgccgtttc
     4261 actccgagtc acaaggagca atttcaaaaa atagcttctc atgtttatta aatattgtga
     4321 ctggtgaccg aggagctggc aggagaacac cgtttattgg agaggttgtg aaaaagctgt
     4381 tctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcatttggtt
     4441 ggacttatta ccgcagatgc aagcttttgg acaccgttta ccaagaggtt gtgaaatcag
     4501 cgttttgacc tgagtattag agaaacaaac ggcaaaatag ttcctcatat tttgccgttt
     4561 tttgagctaa taccgaaggt cagctgattg aacaccgttt attcaaaaaa aatggactgc
     4621 gacacgactt ttgtcacagt cccttcgtgt gtttttctgc cttctcaaaa ggatgacagt
     4681 ttctcactct tttcctattt cttctattca atcaaataac tcattttata tacatctaaa
     4741 aattcaccta gatctgttct tgcccctcta ggcatgaccg cttcgatttg gaagcccatt
     4801 tttcggtaaa ggtgtacggc tctttcatta cgaacttgaa cgtcaagctc caatcgaaaa
     4861 agtacaccca tttccttcgc ccaactgatg atttcttcta gcataagagt gcctagtccc
     4921 attccccaat attcctgcag aatactgata ccgacttctc ccacgtgaga caaacgaaat
     4981 tgactgtctg ccttgacaga tgctgttcca ataatcgtcc cttcatagat tgcaagcagc
     5041 aataaattat tattgctttc gtatatataa tccaactctt cttctaaggt ttctggtggt
     5101 aacaggagtt ctgcttcatc caaaactaaa aattctgtct ccttattaac ttgtcccatc
     5161 atcaaaagaa tgtcttttgc atctcctggc actgcttccc gcaagatgat cccaacttct
     5221 tcactcatga ttttcagccc atttttctag aattcgctgt ttgaaggttt cactttgctc
     5281 cccgtatgag attagtttca cataccgctt ttctaaatct gtatcacttt tttctaaaat
     5341 cagttccaaa taatcctccg gcagtgcttc ttctaataga tttccccatt caacaacgga
     5401 aagcccatca ccttcaaaat attcatctaa ccctaaatct gctccgctag ctgctacccg
     5461 ataaatatcc atatgataca acggaatccg accttgacta tattctctaa taatcgtgta
     5521 tgtcggactt ttgatcattt gcgttatccc taatccttgg gcaattcctt tcgtcaacgt
     5581 tgttttaccc gcacctagat caccagtcaa tatcagattg tcacctggca ttgcggaatg
     5641 accaattgcc tctgcgaatt gttctgtcat tgttaatcct gatagttcga tcatttttca
     5701 tcttctcccg ctcttctttc tttttgccag tatatcgcgt aatgaagttt ttttaaagaa
     5761 aaaatgaact agtcaagaac agcagctgct gtttttggct agttcgcgtg tatttgttaa
     5821 tcaatcaatg tttgtgctgc tgtgataatc gataatttat atacatcttc ttcatttgct
     5881 ccacgagaca aatcagaaac cggtttgttc aagccttgta gaattggtcc gattgcttca
     5941 aagttaccga aacgctgagc aattttatag ccgatatttc ctgactgcaa ttctggaaat
     6001 acaaagactg ttgcttgacc agctacttct gaatctggcg ctttcagttg tgcaactgaa
     6061 ggaacataag aagcatcaaa ttgcaattcg ccgtcgatca tgtattgtgg cgctagtttt
     6121 ttagcgattt tagttgcttc aactactttg tctacttctg gcgatttcgc tgagcctttt
     6181 gtcgagaagc tcatcattgc tacttttggt tcgatatcga acaattctgc tgtttttgca
     6241 gaatctacgg cgatttcagc caattcttgc gcagttggat tcacgttgat cgcacaatcc
     6301 gaaaagacat atttttcttg atctcttcca cgtaccatga tcatcgctcc gcttgtccga
     6361 ctgatgccag gctttgtttt gatgatctga agtgctgggc gaacagtgtc gccagtcgaa
     6421 tggatcgctc cagagaccat tccgtctgca atccccatat acgtaagcat cgtaccaaag
     6481 taattcacat cttttaaaat cttttctgct tgttctttgg atgcctttcc atttctacgt
     6541 tcaacaaaag tatccaccat ttcatcccac ttatcgtaat tatcagggct gatgatcgta
     6601 aagctagatg ttttgattcc tctggcatgc gctgcatttc ttatttcttc ttcgttccct
     6661 actaaaatgg gttccacaag ttcttctgct ttcaaccggg cagctgcgcc taaaatacga
     6721 gcatcagtag cttccgggaa aacgattttg atcccacggc gaacaatttt aaatcttaaa
     6781 ctatcaaaca attccacaag atgacctcca gacgttttta tatgtattat catacactat
     6841 tccagaaaaa aagaacagtt tattttatct gtatcagtca aaaatgctgg aaaaattaag
     6901 tttttcacat acaaagcgaa ctgcactata acaggaaagc gctgttttat tacactgttt
     6961 caggcagttg ccaatcaatc ggttcttctc ctaacgcaag gagcgcatca ttcgttttcg
     7021 agaagggttt ggaaccaaaa aagccacgac ttgcagataa cggactggga tgtggagatg
     7081 ttatgatgat atggcgactc ttatcgatca ttttgatttt ttcttgtgcc ggttttcccc
     7141 acaagataaa cacgattggt ttttcccgtt catttaattt ttcaatgatc gtgtctgtta
     7201 atctttccca acctttcccg cgatgagaat acgcttgccc ttcccgaacg gtaagaacgg
     7261 tgttgagtaa aaggactccc tgtttagccc atgacactag atttccatgt tgaacaggtg
     7321 cgattccaag atcatcataa agctctttat agatatttct taacgatggc gggatcttta
     7381 cgccaggttg cacagaaaaa gataagccat gagcttgatt ttctccatga taaggatctt
     7441 gtcctaaaat caccacttta acttcttcat aaggagtcaa ttccaatgct tcatagatat
     7501 gatacatatc tggatggatc ttttgggacg catattcttt ttttaaaaat tctcttagat
     7561 gcagataata atcttttgaa aattctgagg acaaaacttc ttgccaactg ttatgaatga
     7621 tttctttcaa aaactccacc tcctaataaa aagaataact cacattttct tcaatcacaa
     7681 gggacaatct tcaccctcag cacttgatta atatggtaaa ctaggaacaa gaagcagaaa
     7741 ggaacgataa tcattatgat caaattaatt gcatcagata tggacggcac attattagat
     7801 gcccatatga gtgtttctcc tgaaaatgcc gaggcgatac gttttgcaaa tgatgccggt
     7861 gttgagttca tggtcgctac tggtcgaaac tatcaagaag ctcgcgcagc tttagatgag
     7921 gtaggaatcg attgtgcgat gatcactttg aacggggcac aggtatttga taaagaagga
     7981 aattcattgt ttaccgtgtc gattccaaat aatcaagcaa tctctgtttt agatattcta
     8041 gatgctaacg gtatttacta tgaagtagca accaacgatg gtctttattc tgaaaatcaa
     8101 gcgaaacgaa ttgaaagttt tgcttccatg gtggctaccc atttacctca ccttacgtat
     8161 aaaatggcga ttgccatggc ctcagctaag ctggagttat tgcacatcac atacgtggac
     8221 agtattcgtg ctattttgga agaaaagaat ctggaagttt taaaaattat ttgttttcat
     8281 acagaagggc cagctgtatt aggtccagtt ggaaagcaga tttctctttt aggcgatcta
     8341 gccgttactt cctctggtca aaacaatatc gaagtgaacc ataaaaacgc tcaaaaagga
     8401 attgctgttg cccatgtcgc tcacgagcgt gggatttctt tagaagaagt catgacgatt
     8461 ggcgataact tcaatgatgt cagcatgctg caagtagcag gagtcagttt cgcaatggga
     8521 aatgcagaaa tcgaagtaaa agattatgcc aaatatacaa cggacacaaa ccttgaatct
     8581 ggcgtaggaa aagccatatt gcgcgcgatt gcagaagact tgtagtttta tctagaaaag
     8641 agggatatag atgtcagaat ttttcattca ggaaaagcag cttggacgtg caactcggac
     8701 aattgtcaaa aacgaaaaag gacattccct gtttttgatg gttggtcgct ggggaacaaa
     8761 gggcgatgca ttgtctcttt atgccatgaa tggcgatctc gttgctcaca tcaagcagat
     8821 aagcctgaca ttcggtacgc gttttgagct gtataaagag ttcgaaaagg tgggaaccat
     8881 gcagaaaata ttcaattggc ccggtgactt ttattatatt cgtagacttc actggacagt
     8941 ccaaggtgat atctataatc atcaatatca aatccatcat ttcaatcagc aaatcatgaa
     9001 aatggataaa gcaacacttt tttcaggaga ttactatgta ttggatgtct caaatgatga
     9061 agatgctcca gtttgtattt gtattgctgc ggttttagat tactggctct accgtaaaga
     9121 caaaagtcgc gataagcctg tttcttaccg acttaactta agttaaaaag atgtacctga
     9181 aatgcggaaa tacgcatttc aggtacatct ttttagatat tcttttgcat atgatcataa
     9241 agatgaccac tgatcatccg ctgaccgaca actttgaagt catggcgttc gtataatttt
     9301 ttagcctttg gatttttttc atccacactt agaccgatga cggagcgatt agctttcttc
     9361 gccaatttag gtaatgcttc taataatcgc gaaccgatgc cttgtcctct aaaatcttct
     9421 gatacactga tagagtccaa ataccattcg ttgggaaacg tttctttatc aatgaacaaa
     9481 cgtgtagacg catctagctg atatttctct aagataggta ccagtggctg atcgatgatt
     9541 gattcttctt catctgtata accgtaagca actcctgcaa ctttaccgtc tatttcttct
     9601 acgatacctc gtgaataact gtaacgataa tttggatcag tgattgcttc ttctaaaatc
     9661 tctaaagtct tttgttcccc tgtctgcttc aaaaatggca gttccatatc ttttagaatc
     9721 actaagatca atggggcgat ttctggagca tcttcttttc tcgcaaaacg aatcatagtt
     9781 tcctctcctt ttctccattc taacatgcag aaaaagaaat cgcaaaatcg ttattttaaa
     9841 aaatcttcaa tcttttcagt aatgaaataa ccatgagtaa cgattcttgc cgcttcagtt
     9901 cggcaataag ggcagacgac tggcatttct ttatcattat gttcgactac attattgact
     9961 tcttcgtatg caaatttttt tccgcaatta gggcatttca ttttttccat gttatctcaa
    10021 cttcctttca tctttactct cttagcttac tcttatattg taaaaaagag aagcaatatc
    10081 cttcttctat gcagatatcc atacctgacg gtcctgttct ccatctatta atcggacttt
    10141 ataataagga cgttcatctt tgactagaag tgtccactct ttcacttttg cttttttccc
    10201 gtagtagctg actgcttcct tcatcgctgc ttgcggttgt ttgaactctt ccgttttttc
    10261 tatatcaaaa agatgttctg cggccttttc cgtgtcattt cgtacggttg ttttcccagt
    10321 ggatggattg actgctatga cttgtccttc acaaaaaaac tggtattcat atgttgcatt
    10381 cgtaggattc tccaaatctt tttcaactgc aaaatgaata gcagatatgt tatgttcttt
    10441 tgtttcgttt tttacgattg ctgctgcttt tttcaatgag atccgatatt cctggtcact
    10501 aatatcatcc atgtgaaaag cggcattgag cagtcccgtc ataccggtaa tccctattac
    10561 gatgagggca gcaacaccaa acatcatttt tttcattctt cattctcctg tttctttttt
    10621 ttaaagaata tcaagaaatg aacgaatgaa aaatcgaatg aaacgaaaaa aaagtgacaa
    10681 ttttgttatc tttattcgtg cagttctaat ggcaatccgt ctggatcaaa gaaaaacgtc
    10741 attttttttc ctgtaaaggt atcttctcta atcggttctg attcgatccc taaggactgc
    10801 aattctgcaa ttgtttcttc aatatcgtct actttgaaag caagatgacg caagccacaa
    10861 gcttctggat agtttggtcg ttgtggcgca ttagcaattc caaaaatctc caattcagca
    10921 cttcctagtt tcagatctaa tttatagtca tctcgttctt tgcggtaatt ttctctgatt
    10981 acttcaaatc ctaatttatt aacataaaaa tcacgtgatt tctgataatc tgaaacaatg
    11041 atggctatat gatggatcgt tgtaaggttc atatgagcac tcctattctt cgtttaagct
    11101 cggatctata ttaatctcca gttccattgt aaatgtgtca gattgaaaaa aatagctgtt
    11161 tttccttttt ataagaaaaa aggcaaaaaa aacagaaagt aggtcctcca agacactctt
    11221 cctgttccca atataataaa tgaaagtaag aaattatatt ttcaagaaaa caaacagaaa
    11281 attatcataa cacaaattat ttataattga aagactagtt ttttttcaag aaaagcatac
    11341 aatagattgt gttagtaaat acagaacaca ctacatttag tgtcttaagg agtcctcata
    11401 catgaagtca tattttcagt ttttagctca tcagctaaaa ggctggccgc aacaaaatta
    11461 ttatcttttc tttttcagtc ttggctgcca ggtcatgact cttgtcaata gtcccatcac
    11521 ttggctgtcc gttattacat ttatcggtac cacgcttggt gtattgtgta ttttatccat
    11581 caatgcggca aaatcagtca acggtatttt aggtattttg tctgcgattt gttttattat
    11641 cgttgggttt tcagcaaaga actatttaag tatcggtgaa cagattgctt atatgatcac
    11701 attggatatc cccgtattgt taagtaaaga atggaatcat aatatggctt caaaaatccg
    11761 aaaattcact ggaaaaagct ggtttatcgc aatcatcagt actttccttg tctatcttgt
    11821 ttcaggattc ctgattggtc gttatacaga tgatcctcgg ccgtgggtag atgccattgc
    11881 tttttctatt agcttgactg ctggtatcat cagtttcttg cgttacaaca accaatatta
    11941 ctggtggtta gcttcagggc ttgctcaaat ggtcctttgg tatatttcat ttacgcatgg
    12001 atctgcaagt ttggcgatgt tcgtgaacag ctctgtttac ttgctgaatg atgtccttgc
    12061 atttaccgtt tcaccttggt ataaccaaaa agaacgtgaa cgtatgcaaa aaatggaaga
    12121 agaatactac aacgcacaac aacacgcata aaaaaactga gcgtgggaca aaagtaaaga
    12181 acacttttgt ctcacgctct aaacacgtat aaacggcgga atcagaagca actccttcgg
    12241 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct
    12301 tggagttaaa cgcttctgtc ccagcctcta tttcctacga ttagtcaagt aaaaggtgca
    12361 agaaaaatag ctcgtaaacg aaagcgacaa aatcaaagca caccaaataa agccccgttc
    12421 gtaaaaaatt ttcgatctgg gcaaagaagt ggtatactgt aagtggttta gttaatggtc
    12481 cactgagacc gcgtacgcat agtcgtactc tgtatgtgcc ctaaccatag cgtgcataca
    12541 tttcagaagc ttgttcatac aagcaactgt ggcaacctta tctttcttag ggataggttg
    12601 cttttttaat ttgtagtaat aatcgacaat atgattagga gctgcgcgtt gctggcgaat
    12661 catatttcga atggtgaaga aaataatttt ccgtgctttg ggattaccac gcttattgat
    12721 gcggtcttgg ccagtatact tacctgaatg ataccgtcgg atgtcgattc cgacaaaggc
    12781 attcaattgt ttatgggttt taaatcgtgt gatatctcct aattcaccga gcaataaagc
    12841 ggctgtgagt gcgccaatcc cagggaacgt ttggtacaac aaaaagcaag gagagccttc
    12901 ggcttttttg a
//
LOCUS       DANKMN010000083        12740 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000083 DANKMN010000000
VERSION     DANKMN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            99..542
                     /locus_tag="PW224_002416"
     CDS             99..542
                     /locus_tag="PW224_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2909070.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(548..1732)
                     /locus_tag="PW224_002417"
     CDS             complement(548..1732)
                     /locus_tag="PW224_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL2909071.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2000..2698
                     /locus_tag="PW224_002418"
     CDS             2000..2698
                     /locus_tag="PW224_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2909072.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2705..3382
                     /gene="nth"
                     /locus_tag="PW224_002419"
     CDS             2705..3382
                     /gene="nth"
                     /locus_tag="PW224_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL2909073.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3508..4041)
                     /locus_tag="PW224_002420"
     CDS             complement(3508..4041)
                     /locus_tag="PW224_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909074.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4338..6719)
                     /locus_tag="PW224_002421"
     CDS             complement(4338..6719)
                     /locus_tag="PW224_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2909075.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6709..7356)
                     /gene="recU"
                     /locus_tag="PW224_002422"
     CDS             complement(6709..7356)
                     /gene="recU"
                     /locus_tag="PW224_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL2909076.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7420..7959
                     /locus_tag="PW224_002423"
     CDS             7420..7959
                     /locus_tag="PW224_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL2909077.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8049..8468
                     /gene="gpsB"
                     /locus_tag="PW224_002424"
     CDS             8049..8468
                     /gene="gpsB"
                     /locus_tag="PW224_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL2909078.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8490..8857
                     /gene="rnpB"
                     /locus_tag="PW224_002425"
     ncRNA           8490..8857
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PW224_002425"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9066..10232
                     /locus_tag="PW224_002426"
     CDS             9066..10232
                     /locus_tag="PW224_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL2909079.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10278..11774
                     /locus_tag="PW224_002427"
     CDS             10278..11774
                     /locus_tag="PW224_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL2909080.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11855..12538
                     /locus_tag="PW224_002428"
     CDS             11855..12538
                     /locus_tag="PW224_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2909081.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4273 a         2230 c         2630 g         3607 t
ORIGIN      
        1 tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg aataggaaac
       61 aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga caactgctaa
      121 tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca gaaatggaaa
      181 aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc gggaaaatcc
      241 ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga gccatcagta
      301 agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc gaagagaata
      361 aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat agacacgagc
      421 aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg tcaaaagaag
      481 aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga agtagcaaat
      541 gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa aatccgaata
      601 ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg ccgatgacgc
      661 gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg ttatctgaga
      721 cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg aaaccataac
      781 agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat gtgatgaata
      841 gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct gaaaattttt
      901 tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca atcaaagaga
      961 agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat tgagaaaaga
     1021 tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat aaaggaataa
     1081 cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg gagaaactta
     1141 aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag ccataaacgg
     1201 catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc aactcagcta
     1261 atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca cccatccatc
     1321 caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca tataaggtgg
     1381 agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc catatcgaat
     1441 ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata ataaggatcg
     1501 gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag aaggcagcta
     1561 gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg gagtgagaat
     1621 aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat ccagtgaata
     1681 agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac atgtaaaaac
     1741 tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt ttgtttcctt
     1801 gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa agtgaagata
     1861 cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc tgaccgatca
     1921 gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac gtatactgtg
     1981 attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg gacaaacagt
     2041 catatctaat cttttactta aaaattatca taggatcggt ctacaatctg aagaattttt
     2101 atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg atttgcagat
     2161 gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg atcgtctcgt
     2221 aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag cagatcgata
     2281 taatctttac cccatctatg atctattagg tgagtattta aaaacacaag aaaaaaaata
     2341 cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg aacaagaatt
     2401 tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag aagaagacca
     2461 ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc aggcttatag
     2521 ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac gtacaaaaca
     2581 acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag aagtagaaca
     2641 gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg aggagtagcg
     2701 gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg gaatgtttcc
     2761 cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg cagtgatctt
     2821 aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt ttgcctcttt
     2881 tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta aaataaaaac
     2941 gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc agctgattga
     3001 acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc ctggtgtagg
     3061 cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa tcgcagtaga
     3121 tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg ctacggtcat
     3181 ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa agacacatca
     3241 tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt gcgaggtatg
     3301 tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag aaaaggcgct
     3361 aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg tatgaattat
     3421 cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa gtaaatgtac
     3481 atactcagta gctaggatgg taacttatta caatttattg cgataaaaat aaaagttgtt
     3541 atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac ctataaaata
     3601 agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag gtgtaataaa
     3661 aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg
     3721 cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag
     3781 aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac
     3841 agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca
     3901 aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg
     3961 gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt
     4021 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
     4081 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
     4141 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
     4201 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
     4261 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
     4321 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
     4381 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
     4441 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
     4501 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
     4561 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
     4621 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
     4681 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
     4741 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
     4801 gattctgaag tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa
     4861 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
     4921 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
     4981 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     5041 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     5101 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     5161 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     5221 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     5281 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     5341 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     5401 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     5461 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     5521 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     5581 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     5641 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     5701 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     5761 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     5821 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     5881 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     5941 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     6001 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     6061 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     6121 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     6181 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     6241 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     6301 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     6361 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     6421 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     6481 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     6541 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     6601 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     6661 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     6721 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     6781 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     6841 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     6901 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     6961 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     7021 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     7081 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     7141 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     7201 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     7261 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     7321 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     7381 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     7441 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     7501 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     7561 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     7621 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     7681 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     7741 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     7801 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     7861 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     7921 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     7981 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     8041 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     8101 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     8161 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     8221 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     8281 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     8341 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     8401 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     8461 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     8521 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     8581 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     8641 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     8701 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     8761 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     8821 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     8881 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     8941 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     9001 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     9061 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     9121 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     9181 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     9241 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     9301 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     9361 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     9421 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     9481 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     9541 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     9601 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     9661 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     9721 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     9781 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     9841 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     9901 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     9961 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
    10021 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
    10081 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
    10141 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
    10201 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
    10261 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
    10321 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
    10381 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
    10441 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
    10501 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
    10561 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
    10621 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
    10681 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
    10741 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
    10801 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
    10861 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
    10921 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
    10981 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
    11041 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
    11101 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
    11161 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
    11221 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
    11281 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
    11341 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
    11401 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
    11461 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
    11521 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
    11581 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
    11641 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
    11701 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
    11761 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
    11821 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
    11881 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
    11941 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
    12001 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
    12061 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
    12121 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
    12181 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
    12241 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
    12301 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
    12361 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
    12421 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
    12481 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
    12541 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
    12601 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
    12661 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
    12721 gcaggagaac accgtttatt
//
LOCUS       DANKMN010000084        12738 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000084 DANKMN010000000
VERSION     DANKMN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="PW224_002429"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="PW224_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2909082.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="PW224_002430"
     CDS             complement(322..891)
                     /locus_tag="PW224_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL2909083.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="PW224_002431"
     CDS             1077..1979
                     /locus_tag="PW224_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2909084.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="PW224_002432"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="PW224_002432"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL2909085.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="PW224_002433"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="PW224_002433"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL2909086.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="PW224_002434"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="PW224_002434"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL2909087.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="PW224_002435"
     CDS             8547..8732
                     /locus_tag="PW224_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL2909088.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="PW224_002436"
     CDS             complement(8794..9990)
                     /locus_tag="PW224_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2909089.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="PW224_002437"
     CDS             complement(10160..11596)
                     /locus_tag="PW224_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2909090.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="PW224_002438"
     CDS             11889..12698
                     /locus_tag="PW224_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2909091.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattt
//
LOCUS       DANKMN010000085        12432 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000085 DANKMN010000000
VERSION     DANKMN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..190)
                     /locus_tag="PW224_002439"
     CDS             complement(<1..190)
                     /locus_tag="PW224_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909092.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="PW224_002440"
     CDS             complement(200..1015)
                     /locus_tag="PW224_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2909093.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="PW224_002441"
     CDS             complement(1277..1720)
                     /locus_tag="PW224_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL2909094.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="PW224_002442"
     CDS             complement(1854..2192)
                     /locus_tag="PW224_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2909095.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="PW224_002443"
     CDS             complement(2180..2557)
                     /locus_tag="PW224_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2909096.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="PW224_002444"
     CDS             2781..3974
                     /locus_tag="PW224_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2909097.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="PW224_002445"
     CDS             complement(4140..4562)
                     /locus_tag="PW224_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2909098.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="PW224_002446"
     CDS             4957..5151
                     /locus_tag="PW224_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909099.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5594
                     /locus_tag="PW224_002447"
     CDS             5148..5594
                     /locus_tag="PW224_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909100.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5804..6976)
                     /locus_tag="PW224_002448"
     CDS             complement(5804..6976)
                     /locus_tag="PW224_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2909101.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="PW224_002449"
     CDS             complement(7182..7997)
                     /locus_tag="PW224_002449"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2909102.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="PW224_002450"
     CDS             complement(8147..8593)
                     /locus_tag="PW224_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2909103.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..9594)
                     /locus_tag="PW224_002451"
     CDS             complement(8590..9594)
                     /locus_tag="PW224_002451"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2909104.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9634..9963)
                     /locus_tag="PW224_002452"
     CDS             complement(9634..9963)
                     /locus_tag="PW224_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2909105.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10089..12416)
                     /locus_tag="PW224_002453"
     CDS             complement(10089..12416)
                     /locus_tag="PW224_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2909106.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3513 a         2430 c         2272 g         4217 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag ctaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9361 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9421 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9481 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9541 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9601 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9661 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9721 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9781 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9841 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
     9901 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
     9961 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10021 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10081 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10141 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10201 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10261 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10321 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10381 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10441 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10501 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10561 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10621 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    10681 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10741 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10801 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10861 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    10921 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    10981 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11041 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11101 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11161 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11221 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11281 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11341 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11401 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11461 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11521 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11581 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11641 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11701 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11761 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11821 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    11881 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    11941 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12001 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12061 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12121 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12181 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12241 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12301 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12361 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12421 cctctcaata aa
//
LOCUS       DANKMN010000086        12226 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000086 DANKMN010000000
VERSION     DANKMN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..566
                     /locus_tag="PW224_002454"
     CDS             <1..566
                     /locus_tag="PW224_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDL2909107.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /locus_tag="PW224_002455"
     CDS             967..1572
                     /locus_tag="PW224_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL2909108.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="PW224_002456"
     CDS             1588..2160
                     /locus_tag="PW224_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2909109.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="PW224_002457"
     CDS             complement(2412..2669)
                     /locus_tag="PW224_002457"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909110.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="PW224_002458"
     CDS             complement(2795..3610)
                     /locus_tag="PW224_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909111.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="PW224_002459"
     CDS             4127..5452
                     /locus_tag="PW224_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2909112.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="PW224_002460"
     CDS             5445..5795
                     /locus_tag="PW224_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909113.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5792..5962
                     /locus_tag="PW224_002461"
     CDS             5792..5962
                     /locus_tag="PW224_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909114.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6866..7141)
                     /locus_tag="PW224_002462"
     CDS             complement(6866..7141)
                     /locus_tag="PW224_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL2909115.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="PW224_002463"
     CDS             complement(7113..8066)
                     /locus_tag="PW224_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2909116.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="PW224_002464"
     CDS             8678..10171
                     /locus_tag="PW224_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2909117.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="PW224_002465"
     CDS             10306..10602
                     /locus_tag="PW224_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2909118.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="PW224_002466"
     CDS             10703..11554
                     /locus_tag="PW224_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909119.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11858
                     /locus_tag="PW224_002467"
                     /pseudo
     CDS             11575..11858
                     /locus_tag="PW224_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4205 a         1873 c         2286 g         3862 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca cctggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaacggttc tgttgcaaag ttttaaatct actatcaaat
    12181 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatc
//
LOCUS       DANKMN010000087        11969 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000087 DANKMN010000000
VERSION     DANKMN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..799
                     /locus_tag="PW224_002468"
     CDS             <1..799
                     /locus_tag="PW224_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDL2909120.1"
                     /translation="NKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQE
                     LELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIA
                     YMQKANADVEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGE
                     DIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNK
                     INQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            871..1992
                     /locus_tag="PW224_002469"
     CDS             871..1992
                     /locus_tag="PW224_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2909121.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2074..3846)
                     /locus_tag="PW224_002470"
     CDS             complement(2074..3846)
                     /locus_tag="PW224_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2909122.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3849..5561)
                     /locus_tag="PW224_002471"
     CDS             complement(3849..5561)
                     /locus_tag="PW224_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2909123.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5676..6344
                     /locus_tag="PW224_002472"
     CDS             5676..6344
                     /locus_tag="PW224_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL2909124.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6411..7199)
                     /locus_tag="PW224_002473"
     CDS             complement(6411..7199)
                     /locus_tag="PW224_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL2909125.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7282..8754)
                     /locus_tag="PW224_002474"
     CDS             complement(7282..8754)
                     /locus_tag="PW224_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2909126.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8884..10077)
                     /locus_tag="PW224_002475"
     CDS             complement(8884..10077)
                     /locus_tag="PW224_002475"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL2909127.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(10075..10154)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10343..10414)
                     /locus_tag="PW224_002476"
     tRNA            complement(10343..10414)
                     /locus_tag="PW224_002476"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10379..10381),aa:Glu,seq:ttc)
     gene            complement(10426..10499)
                     /locus_tag="PW224_002477"
     tRNA            complement(10426..10499)
                     /locus_tag="PW224_002477"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10463..10465),aa:Ile,seq:gat)
     gene            complement(10512..10582)
                     /locus_tag="PW224_002478"
     tRNA            complement(10512..10582)
                     /locus_tag="PW224_002478"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10548..10550),aa:Gly,seq:tcc)
     gene            complement(10587..10659)
                     /locus_tag="PW224_002479"
     tRNA            complement(10587..10659)
                     /locus_tag="PW224_002479"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10624..10626),aa:Phe,seq:gaa)
     gene            complement(10663..10735)
                     /locus_tag="PW224_002480"
     tRNA            complement(10663..10735)
                     /locus_tag="PW224_002480"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10700..10702),aa:Asp,seq:gtc)
     gene            complement(10754..10827)
                     /locus_tag="PW224_002481"
     tRNA            complement(10754..10827)
                     /locus_tag="PW224_002481"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10791..10793),aa:Met,seq:cat)
     gene            complement(10840..10929)
                     /locus_tag="PW224_002482"
     tRNA            complement(10840..10929)
                     /locus_tag="PW224_002482"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10891..10893),aa:Ser,seq:tga)
     gene            complement(10952..11023)
                     /locus_tag="PW224_002483"
     tRNA            complement(10952..11023)
                     /locus_tag="PW224_002483"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10988..10990),aa:Glu,seq:ttc)
     gene            complement(11045..11132)
                     /locus_tag="PW224_002484"
     tRNA            complement(11045..11132)
                     /locus_tag="PW224_002484"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11096..11098),aa:Ser,seq:gct)
     gene            complement(11157..11230)
                     /locus_tag="PW224_002485"
     tRNA            complement(11157..11230)
                     /locus_tag="PW224_002485"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11194..11196),aa:Ile,seq:gat)
     gene            complement(11244..11314)
                     /locus_tag="PW224_002486"
     tRNA            complement(11244..11314)
                     /locus_tag="PW224_002486"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11280..11282),aa:Gly,seq:tcc)
     gene            complement(11316..11391)
                     /locus_tag="PW224_002487"
     tRNA            complement(11316..11391)
                     /locus_tag="PW224_002487"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11356..11358),aa:Asp,seq:gtc)
     gene            complement(11410..11483)
                     /locus_tag="PW224_002488"
     tRNA            complement(11410..11483)
                     /locus_tag="PW224_002488"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11447..11449),aa:Met,seq:cat)
     gene            complement(11496..11585)
                     /locus_tag="PW224_002489"
     tRNA            complement(11496..11585)
                     /locus_tag="PW224_002489"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11547..11549),aa:Ser,seq:tga)
     gene            complement(11609..11682)
                     /locus_tag="PW224_002490"
     tRNA            complement(11609..11682)
                     /locus_tag="PW224_002490"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11646..11648),aa:Ile,seq:cat)
     gene            complement(11696..11769)
                     /locus_tag="PW224_002491"
     tRNA            complement(11696..11769)
                     /locus_tag="PW224_002491"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11733..11735),aa:Met,seq:cat)
     gene            complement(11797..11869)
                     /locus_tag="PW224_002492"
     tRNA            complement(11797..11869)
                     /locus_tag="PW224_002492"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11834..11836),aa:Ala,seq:tgc)
     gene            complement(11889..11962)
                     /locus_tag="PW224_002493"
     tRNA            complement(11889..11962)
                     /locus_tag="PW224_002493"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11926..11928),aa:Pro,seq:tgg)
BASE COUNT         3878 a         2527 c         2079 g         3485 t
ORIGIN      
        1 caataaacta actatttcta atatagactc gactagggaa aaaatcataa aaatatttga
       61 taaaattaat aatttattca agaatgacaa tgctgaatta aggaagggtc ttttagagtt
      121 taaacttcaa gaactagaac ttcattactc ttatttagtg aaaattaccg aagaaaaaga
      181 acaacaaaaa gcaataaaag aacagatgat agaagaggaa aaagttcgta gagaaattga
      241 tcgtgaaaag aaacgtattg ataaagaaca acgtcaattc aattctgaaa tttccaaatt
      301 aatcgcatat atgcaaaaag ctaatgcaga tgtggaaaaa gaattatatg caaataaaat
      361 tcaagaactg gaagaaaaat taaaagaatt agaagttata cgtgaaaatg tattacaaag
      421 agaattaaat actagagccg gttatgtgta tgtaattagt aatattgggt cttttggtga
      481 agacatatat aaaattggta tgactagaag attagaacct atggatagaa taaaagaatt
      541 gagtagtgca tcggttccat ttgaatttga cgttcatgcg atgatttttt cagaggacgc
      601 tccgtctttg gaaactaaat tgcataatca ttttagaaaa caagaagtta ataaaattaa
      661 tcaaagaaaa gaatttttca aagtttcact agacgaaata gagaaagttg ttttagaaaa
      721 ttataatgga acagtaacat ttacaaaatt agctaaagct gaacaatata gacgatcttt
      781 agaattgtca aaagattaaa tatataaaaa gccttcgggc tttttctttt aacaattaat
      841 aggaacgtat attcgaaagg aatagaaata atgtggttag aaaaaataaa tgacaaaaaa
      901 tttaagtaca ccgaacgtta tacggatccc ttaactgaaa aaaagagaaa agtgtccgtt
      961 actttatcta gcaattcacg ccaagcttgg aatcaagcta acttactttt gaatgaaaaa
     1021 atcgcagaaa aaataaaacg aaatgaagaa ttaccactaa cttttggaga attaaaaact
     1081 aaatgggatg aaaaatacaa gccaactgta aaagaaagtt cctatcgaac aactcaggtt
     1141 tatctatcat taatttcaaa atatataaaa gatgatgtac ttgtaaaaaa cgttaatagt
     1201 aatcttatac aagatatgct agattactac tattttgaaa aaaatttatc atataattat
     1261 gtaatacatt taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga
     1321 ttaaaatata atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa
     1381 ataaaaaaag aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa
     1441 caaaaaagtt cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact
     1501 ggactacgtt atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt
     1561 acggtttctg gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt
     1621 tcttatagaa ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa
     1681 gaaaacaaat taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac
     1741 ccgatatcta ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaaccta
     1801 gataaaaaag tttcttctca catgttaagg cattcccaca tttctctact tacagaatta
     1861 ggaatcccat taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta
     1921 aagatttata ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa
     1981 atagaaattt aaattgcccc ttttctgccc cttataaaaa caaacaacat aaaatatata
     2041 aaaagaaccc cgttataacg aggttctttt aatttaactt tcaaatacaa attgattatg
     2101 ataaagttcc gcataaaatc cattttctgc taataactca tgatgactgc cttcttcaat
     2161 gacttcccca tcacgcaaga caacgattct atctgcattc aagattgttt tcagccggtg
     2221 agcaatcaca aagctggttc ttcctttgat tgcttcatcc attgcttttt ggatccttgc
     2281 ttctgttacc gtgtctacat tacttgttgc ttcatccaaa atcaataaag atggatttgt
     2341 gatgatcgtt cgagcaatac tgatcaactg cttttgacct gtactgaata agttattttc
     2401 ttcggatatc tctgtatcat aacctttttc taacgtcatg atgaagtcat gaatattcgc
     2461 ttgttttgct gcattgatga cttcttcatc tgaagcgtcc ggtttgccga atgtgatatt
     2521 gtctttgatc gttccggaga acaatacaga ttcttgcaaa acgattccta catgtctacg
     2581 taatgcatca agatcataat ctcggatatc tattccatcg atgactactg aaccatcatt
     2641 gacatcgtaa aaacggttta gcagattcat gatcgtcgtt ttccccgatc ctgttggacc
     2701 aacaagagca accatttctc ctttgtccac attgatcgag acatctttca agactagttt
     2761 ttctggagaa taaccaaaat ctacgtgctg caacgcaaga cctttttcaa caccatcaaa
     2821 tgctttaccg ttttctggtt tgacttcatc tttttcatca aatatttcat tcaatcgtct
     2881 tgcccctgta atcgctaatt ggatcatgct gtatccagat gaaatctgca tcagtggttg
     2941 gtaatattgt tgtgaatact ggacgaatgt cacaactaag cctaatgcca ccgaacgttc
     3001 caaatcacca tttaatgcta accagccgcc gaaaaagata actatcgctg tattgaccag
     3061 tgacattcct tgcatcatcg gaaatagtag accagaataa gcttgtcctt tgaaggttgc
     3121 ttcgcgaaca ctttgattat gctctaagaa tccttcaatc gtttcttctt gtaagccatt
     3181 tgtgatgatg acacgttgtc cgctgatttt ttcatccata taaccattta gtttccccac
     3241 ttcatcttgc tgaagatcca catatttccg tgctttgcgg ataatcaaga cagctatcaa
     3301 aacggctact ggagtggaag caattgtagc ccaagctaac tctacatttt ggcggaacat
     3361 catgatcaag atcccgacaa gtaaaacagc attcgtgatg acttgaagca aagcttggtt
     3421 caaactattt tgaatattat ctaagtcact ggtaaaacga cttaaaattt caccgtcttg
     3481 atgagaatca aagaaacgga tcgtcaattt ttccaatttg ttgaatagac cgatacgcat
     3541 tcggttcgtt gacttcccta ctacttgtgt gaacaaaata ctatagataa agttggctgc
     3601 actagctagt acataaaaca gcaagagttt ccatattaca gaaacaaatt tgcttttatc
     3661 gtctacgcct tgcatcaagc ctcctacgta attagctaat tcttgaaaag cttccccgat
     3721 gtattgaggt gcctttactt gtagataggt agcaatgatc actgtgataa agataaagaa
     3781 aaaggagagt ttgtaccgct tcaaatagtg atagaaaaat ttacttgctt ttattagatc
     3841 agtcatactt actcctccgt tcctttttga gtttcaaaga tttcctgata gacacggttt
     3901 ccagctacca attcctcatg ggttccttcg ccgactaatc tgccttcatc caatactaag
     3961 atgcggtctg ctttgacaac agaagcaatt ttttgtgcga tgacaatcgt cgttgttccc
     4021 ttcaaatctc gatcgagtgc ttctcggacc agcttttcag agcgagcatc cagtgcactc
     4081 gtactgtcat ctaggatcaa gatctttgga ttaccgataa cgccacgagt aatggataaa
     4141 cgttgctttt gaccaccaga aaagttactg ctacgttctt ctattggtgc ttcgtaacga
     4201 tcagataact tttcgatgaa ttcttttgct tgagcaatag aagaagcatg ttccatctct
     4261 ttttctgttg cattcttttt cccttggcgc aagttttgag caatcgttcc tgagaataaa
     4321 attgcttttt gcaaaacaaa ggaaaccgtt tgtcttaaac tcttttcatt tacttgacga
     4381 agatctacac cacctacttc aactttgcct tctgtcggat caaatagacg agggatcaat
     4441 tgtgctagtg ttgacttccc ggcaccagtc gccccgacga taccaaccat ttctccaggt
     4501 ttgatagaaa agctgatatc tttaagcgta tctgtatcct caccaggata acgaaaagaa
     4561 acatggtcaa aagtgacaga acccgtcaac tcttgttctg gaacgtctaa ataagtaaga
     4621 tctggttcag catctaaaat ttccgcaatc cgcttcagcg atacggctgc tcttgaagtc
     4681 atcatcatca tcattccgcc aataatgatt gccatcataa tctgcatcaa gtagttcatg
     4741 aatgaagcaa ttccaccaat taacgtagga tcatctttaa caagatcgct cacaaaaaag
     4801 atcgccccaa ccacagccaa attggcagcc agcatgaagg acgggatcat cacagaaaat
     4861 aatgtaccga ctatgatgtt gtgttttgtt aagtcctcac ttactttgct gaatgacttc
     4921 aattggtttt cttcttgtac aaaagatttc actacacgaa tccctagtaa gttttctttt
     4981 gcaatgctat tcacacgatc aatcagtttt tgaatgatca tgaagtgctt gcccatcctt
     5041 gtaaatgaaa gtgccgtaat cagaaatacc gtaacgataa gcgctacgat gatccaccat
     5101 aattgcggca tcgtgatcat agctaaaatg aaggcaccaa taaataagaa tggaattcga
     5161 aaaagtgatt gcaaagaaat catcactaaa ttttggattt gcgtgatatc atttgttaga
     5221 cgaacaacaa gatttccagc agagaactgt tcgatatttc caaatgaaaa tgtctggatc
     5281 tttcgaaatg cttcttctcg gatatctgca cttacgcctt gagctacttt tgcagagaaa
     5341 atcgtgttcg ttacacctgc ggctaatcct agcaaagcca gactgatcag gtaaatacca
     5401 attgttttca tttcatcatt atcttctgta ataatagctt ccaatacttg ctgtagtaac
     5461 ttaggttgcc ataatgctgc tgcaaccatg actgctacgg acagcaagga aataaaaaca
     5521 gatactttat attttttcgc atgtcgtaag attaatttca ttcgttgatt acaccccttt
     5581 gtttaaattg acaggactaa tttaacactg taaaattaga ccgactaaca atttaccatg
     5641 tttaaaaaga taatgcaata cacgaggttg ataaaatgga aataaagctt tctcaagcaa
     5701 tcatcattca ggccgccttt gatatcctgg ctgaaacaca agctctttcg cagctttcca
     5761 tgcgaaacat cgcaaacaga attcatgttc aagcacctgc tttatattgg tatttcaaaa
     5821 acaaacaaca gctcttgcaa aaaatggctg aaacaatgga agaagaatta gcgcttccag
     5881 atccaacttt gccttggaat gaacagttgc tgcagtatat ggaaaattac tatgaccttt
     5941 atacacgttt cccttgtggg gcagaattag aaatcaatac tgtgcccgct tttccgagtc
     6001 gattagaaca tttggagcaa atgatccaat tacttgttcg tgaaggattt tcagtcaccc
     6061 aaagtcatca ggccattgcc tctctccaca atttattgat tggacagtta atggatcagc
     6121 agcatgaaca gcagcttcga caaaaaatta tggacggaaa tgaatttttg aaagaaacag
     6181 ttctatttat gcgtaattat gtagttgaaa atcaattaga gggatttaaa gaaagtatgc
     6241 agtcccatgc agaattgaat gaaaaagcac tctttctaac gagtgcacaa acctatattg
     6301 aaggcttatg ccaacaaaat aaaagaagag aaaacgagca gtaaaaaagc tcctatagag
     6361 atcactatgt ttgtgatctc tataggagct atctttttaa gactcttttt ttatttttct
     6421 tccattagcc acgccaatgc acgttgaatc cattggtccc agaagatcca gttatgatcg
     6481 ccaggtcctt gttcaaaggt gaccgccaag ccttgttcct gcatagcaga taccatttcc
     6541 tgatttgcct gataaagaaa atcagattcc ccacaagcca tataaaatct tggaactggt
     6601 tttccgcttt ttactagttc tttcaataag tattgcggat cattttttga accttcgatt
     6661 tgatccaatg gtccaaagat cccttcccaa aaagatgctt tacgaaccat caaaagatca
     6721 tccattcgat ttcctacaga aacagcgcct gaaagagaga ttactgctgc gaaattttct
     6781 gattttgcaa ggcctagttt taacgcgcca tatccgccca tcgataatcc agcggcatat
     6841 gttttttctc tttttcttgt taattgcggg aataattcat gacaaatctc aggtagctct
     6901 tctgcaatga atgtccaata cttcatgtca taaactgtat ctgtgtacca gccaagatca
     6961 gtagatggca tgatgacagc taatccgtag tttgctacat aacgttcaat cgacgtacgt
     7021 ctttcccata cactatgatt gccacccatt ccatgaagta aatataaaac aggtatatcc
     7081 gttgtctctt gctctgaact ggtgccaatc accttttttg ttgtttgcgg tagtatcaca
     7141 tttagattga cttccatttc taacacattc gaataaatat ttgcttgaat aaatgccata
     7201 ttttcgcctt ccttagtaag aagttatttt tgaataactt taaatttgaa tgattcggct
     7261 acgtacatat cttaaccgaa actactgttt tattttacca cttttttcat gaacgaaaag
     7321 cgttaagacc aaagcaatga tcacgagtaa caagccaact agataaacgg aatgtaaccc
     7381 atcataaaga atttttctta atggtaacaa aatatccgca tcaatatatt tagccgtttg
     7441 tggattcacc agtttattca tgatatctgc atcatttgtt aaatttgctg ctgcaagttc
     7501 tcgtttagtt accccattta tcaataaacc aaaaatagaa accatgactg tttgaccaat
     7561 tgttcgaact aacgtattaa aagaagttgc tacaccaagc tggtcacgag atacactatt
     7621 ttgtgcactg acagtcgagg tagtcatggt cacacctaag cctataccga tgaccgttga
     7681 tatagcaaaa aaccataaat agttacttct gaaaggcata agatacaacc aagcgccccc
     7741 aatagctgcg attagtagtc caattctcaa gacccaatga gttttcattt gagtcatcca
     7801 cttgcttgca aggaaagaac cgaccatcca gactagtgat aatggtgcta ataccagccc
     7861 accaattgct gcattcagcc ctaatacacc ttgcatccac atagggatat aaacatcgat
     7921 tcccatcaaa aaaccactag ccaatgctgc agccaagttg accacaacaa atagtggatt
     7981 tttgaataaa tgcatcatga tgacaggatc tttagctctt ttttcggcaa aaacaaaaat
     8041 acctaacatt acgatactac ctagcagcaa agcagaaaca agcggactca ttccttcgtc
     8101 tcctaataat tggaacccta acagcaatga gacaagcatg gccatcaaac taaaactacc
     8161 aaaaatatcc attggtttag actcatgtat gatttttggt tcatttaaat atagccaaat
     8221 caaaataatt aatagaattc cgatcggcac attgataaaa aagatccagt gccagctgat
     8281 cgtttcaacg atgaatcctc ccgcaagcgg tccaaaaaca ctggcaatcc cccaggctga
     8341 actattcaaa cctaaaactt ttgctcgctt ttcaatagaa taaagatcgc cgatgatcgt
     8401 caaagctact ggcatcattg ccccagcacc catcccttga atcgcacgag cagcaatcaa
     8461 cccaatcatg ctttgagata aaccgcaaaa taacgaaccg ataataaaca ataatgtccc
     8521 aaaaataaag attggtttcc gtcccacttt atccgcaagt tttccgtaaa tcggagtaag
     8581 catagcgttt gtcagcaaat aaatcgaaaa tacccagttc atgatttcca ttccatgtaa
     8641 tgaaccgaca atcgttggca tagcggttgt tacgattgta ccttcaatag ccgtcatgaa
     8701 cgttgcaatg aaaatactca ctgtgaccaa agtaacattt gtttcttttt tcatcttatt
     8761 cctccctttc attatgacag gatctaaaaa tagataaata attactatct ttttcaagag
     8821 gttaactgcc ataaaaaaag ctggaacaac caccacttgg cgatcgttcc agctgaatat
     8881 aacttatttt gctaaacttg ctttcaacgc atctactttg tccgtatgtt cccaaggcaa
     8941 atctacatct gtacgaccaa aatgaccgta agcagctgtt tgtttgtaaa tcggacgacg
     9001 caaatcaagc atctcgatga ttcctgctgg ccgaagatcg aagttttctc tcactgcagc
     9061 aatcagttct tcttccggaa ccgttccagt accgaaagta ttgattgaga tcgaaacagg
     9121 ttgagcaaca ccaatcgcat aagctagctg tacttctgct ttgcgagcaa gccctgctgc
     9181 aacaatgttt ttagcaatat aacgtgcagc atagctggca gaacggtcaa cttttgtcgc
     9241 atctttacca gaaaaagcac cgccaccatg acgagcataa ccgccatacg tatcaacaat
     9301 gatttttctt cctgttaatc cggcatcccc ttgaggacca ccaatgacaa atcggccagt
     9361 cggattgata taatatttcg tttgatcatc taataattca gctggaataa cttccttcac
     9421 tactttttcg atgacatcat gacgaattgt ttcattatct actgcatcat catgctgtgt
     9481 tgaaataacg attgtatcta cgcgttccgg ttgtccttga tcatcatatt caaccgttac
     9541 ttgagatttt gcatctggac gtaaatacgt taattcattt gatttacgca aatctgccaa
     9601 gcgtcgtacc agacgatgac tcaaagcgat tggtaaaggc atcaattctg gtgtttcatc
     9661 tacagcaaac ccaaacatta aaccttggtc accagcacca atctcatcta atacgtcttt
     9721 tttatcttca tcccggatct caagtgcttc atcaactcct tgagcaatat caggagattg
     9781 ttcatcaatc gcaaccaata cagctgctgt atctccatca aatccgaatt ttgcacgtgt
     9841 ataaccaatt tcttttattg tttcccgtac gactttttga atatctacat atgccgtcgt
     9901 agatatttct ccaaatacta aaacaagacc agttgttaca gacgtttcac acgctactcg
     9961 tgctgttgga tcttgtttta aaattgcatc caagattgca tcactgattt ggtcagcaat
    10021 tttatctgga tgtccttcag aaacagattc tgatgtaaat aagtgtcttt ctaccattat
    10081 aatgattccc cctagttaaa ttcggttaca aggcatctcc gtcctgcgga tcctttcggg
    10141 aacttgcaac gaaccaagta tagcagataa aacagttttc tgctcgtatt ttttcatttt
    10201 tttgcttaaa aaatataaaa atattttaca ttagaactgt ccaaaaaagc ttagcacaat
    10261 catccataaa gataactctg ctaagctttt taaaatcatt ctttcagata aaaagaaaaa
    10321 gatactcaaa aagtatcttc catgacccgt acgggattcg aacccgtgtt accgccgtga
    10381 aagggcggtg tcttaaccgc ttgaccaacg ggccaataaa ataaatgggc ctaaatggac
    10441 tcgaaccatc gacctcacgc ttatcaggcg tgcgctctaa ccagctgagc tataggccca
    10501 taaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
    10561 tttaccacta aactacaccc gcataatggc gcgggacaga atcgaactgc cgacacatgg
    10621 agcttcaatc cattgctcta ccaactgagc taccgagcca aacggttccg acgggaatcg
    10681 aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg gaaccaatgt
    10741 ttttagtttt taattgcggg agcaggattt gaacctacga ccttcgggtt atgagcccga
    10801 cgagctacca gactgctcca tcccgcgata atatgaaaaa ggaggataag ggattcgaac
    10861 ccttgcacgg ttttacccgc ctgacggttt tcaagaccgt tcccttcagc cggacttggg
    10921 taatcctcca tgtaaaacta tattaaatta atgacccgta cgggattcga acccgtgtta
    10981 ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa acaaaatatt
    11041 gaaacggaga aggagggatt tgaaccctcg cgccggtttc ccgacctaca cccttagcag
    11101 gggcgcctct tcagccactt gagtacttcc ccaaaaccaa taagattttg tactaatggg
    11161 cctaaatgga ctcgaaccat cgacctcacg cttatcaggc gtgcgctcta accagctgag
    11221 ctataggccc ataaaaaact aaaagcgggt gacgagaatc gaactcgcga caacagcttg
    11281 gaaggctgtg gttttaccac taaactacac ccgcatggcg gttccgacgg gaatcgaacc
    11341 cgcgatctcc tgcgtgacag gcaggcatgt taacccctac accacggaac cagtgttttt
    11401 agtttttaat tgcgggagca ggatttgaac ctacgacctt cgggttatga gcccgacgag
    11461 ctaccagact gctccatccc gcgataatat gaaaaaggag gataagggat tcgaaccctt
    11521 gcacggtttt acccgcctga cggttttcaa gaccgttccc ttcagccgga cttgggtaat
    11581 cctccatatg aaacttacaa cgccacaatg gaccttgtag gactcgaacc tacgaccgga
    11641 cggttatgag ccgtctgctc taaccaactg agctaaaggt ccaggctctt aaaaatcgcg
    11701 gcggagggga tcgaaccccc gacctcccgg gtatgaaccg gacgctctag ccagctgagc
    11761 tacaccgcga aaaagatatt cattatttaa atgaaatgga gcctagcggg atcgaaccgc
    11821 tgacctcctg cgtgcaaagc aggcgctctc ccagctgagc taaggccccg tgtttaaatt
    11881 tataattatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc
    11941 taccaagctg agctacttcc cgtttaatt
//
LOCUS       DANKMN010000088        11866 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000088 DANKMN010000000
VERSION     DANKMN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            560..1531
                     /locus_tag="PW224_002494"
     CDS             560..1531
                     /locus_tag="PW224_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL2909128.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1609..1845)
                     /locus_tag="PW224_002495"
     CDS             complement(1609..1845)
                     /locus_tag="PW224_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2909129.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1814..1945)
                     /locus_tag="PW224_002496"
     CDS             complement(1814..1945)
                     /locus_tag="PW224_002496"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2909130.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2021..2404)
                     /locus_tag="PW224_002497"
     CDS             complement(2021..2404)
                     /locus_tag="PW224_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2909131.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2437..3222)
                     /locus_tag="PW224_002498"
     CDS             complement(2437..3222)
                     /locus_tag="PW224_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDL2909132.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3400..4500
                     /locus_tag="PW224_002499"
     CDS             3400..4500
                     /locus_tag="PW224_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2909133.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4490..5314
                     /locus_tag="PW224_002500"
     CDS             4490..5314
                     /locus_tag="PW224_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDL2909134.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5311..6126
                     /locus_tag="PW224_002501"
     CDS             5311..6126
                     /locus_tag="PW224_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2909135.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6123..6953
                     /locus_tag="PW224_002502"
     CDS             6123..6953
                     /locus_tag="PW224_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2909136.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6960..7991
                     /locus_tag="PW224_002503"
     CDS             6960..7991
                     /locus_tag="PW224_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2909137.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8098..9371)
                     /locus_tag="PW224_002504"
                     /pseudo
     CDS             complement(8098..9371)
                     /locus_tag="PW224_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9598..9783
                     /locus_tag="PW224_002505"
     CDS             9598..9783
                     /locus_tag="PW224_002505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909138.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            9907..10623
                     /locus_tag="PW224_002506"
     CDS             9907..10623
                     /locus_tag="PW224_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDL2909139.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10525..11045)
                     /locus_tag="PW224_002507"
                     /pseudo
     CDS             complement(10525..11045)
                     /locus_tag="PW224_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11333..>11866
                     /locus_tag="PW224_002508"
     CDS             11333..>11866
                     /locus_tag="PW224_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2909140.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKD"
BASE COUNT         3404 a         2193 c         2711 g         3558 t
ORIGIN      
        1 ataacgtgat tttttgctgt aatgacaagg ttttagctgt ttcagatacc atgtatccaa
       61 aaatagttta ttgagaaaat aggagagtat ttgatactaa ctagtaggaa aaaatgagat
      121 aaaaagtaaa tggaatcatt taaggtaatc tgaaataaaa aatatacagc cctgtggtac
      181 tttacctaca aggctgtata tattttttac tctcagcgtt tttgactccg tgtctgactc
      241 cgttttattg attttgaatg ttattcaatg accatttaac aggtcaaatt tgatgttttt
      301 gattccgtat gacgccgttt gatatctagt gatatagtga tttgagtcta catcgaataa
      361 gggaataaac caataaaaaa tgatttagaa tattaaaata acaaaaaaga ggactacctg
      421 tattagtcct cttttttgac cagattttga ccagtctatt ttacatccta gtatatttat
      481 atgagctaat tttatgatta aatggtaagt ttttccttgt gtgttttagt ttaaactagt
      541 gtgtaattca aaaatctata tggaaggaac agtattaaga atagaacagg gttctctaca
      601 tgatggtgct ggtttacgga ccgttgttta tttaaagggc tgcccattac gctgtgcctg
      661 gtgttcgatt cccgaatcac aaagcaagca gattgaaaaa ggatttggcc agaccatgac
      721 cgctgaagag gtaatggatg agattgagaa ggatgctgtt ttttactttc actcggatgg
      781 cggagtgacc attagcggag gcgaggcact ggttcaggcg gattttgcta aagaaatttt
      841 gcaaaaatcg aaatacattg gaatcaatac cgtactggaa accagctttt gtggtgctta
      901 taatgaaatc caaaaggtag ctccctatgt ggatacattg tttgtggatg taaaaatgtt
      961 taccagcaag ctccacaaac aatggaccgg cttagataac caacaaattt tgaagaatat
     1021 tcgtcgcttt ttaattgagt atcctaactg cgaagtgcga attcgagttc ctgtggttcc
     1081 aggtatcaat atgaacctga ccgagctgct gacgattgct tgttttgtgg cagatcttga
     1141 tcggttcgta ccgttagaat tactaccgta ccactgctat ggtatgcacg gttaccaagc
     1201 attgggactg gagtatccgt tggcagacac gccagcgcct tcacctaaag aaatgtttgc
     1261 attggcagat caactggctc gcacggttcc tcatttgctg gtcacagctt taaccaaaac
     1321 atttatttat ttattgaatc caagctttat cttatggata aatgaaagac actcactagc
     1381 tgcagccagc tcaagaatag agcttttgct gaagtcagtg agtgtctttt tacgatgttt
     1441 tttcgaagtt aatttgtcga atgaacactg gctcaatcgg tacatcttca ctgtttgtcg
     1501 gctgagctgc gatcgctgct acgatttcta acccttggta tacctgtcca aaaacagtat
     1561 aacgaccatc cagctccggt agtcctccaa gctccaagta tgcttgcttt acattagcag
     1621 gtcagcctgc gcctttgtgt tttgcacaat aaaaaactgg ttgccgttgg tgtgggggcc
     1681 gtggtcccct aaggccaagg cgccattaaa atgataaagt ccgcgatgca cttcatcagc
     1741 gaaaaagccc tcatagctgg aaacagcttc atcaccagaa ccatccaaag cgccaccttg
     1801 aataacaaat tccttaatga cacgagccga aaggtgtgtt gtcataaacg ccttgctcag
     1861 ctagggagcg ccagttggct acagccaggg gagcttggtc aggaaataag cggagacgaa
     1921 tttcaccagc atcggtgacc agcacacaaa cttcttcctg ctgcggtttt tctaattgaa
     1981 agagaggaag gtttgcaact gaaacgcctt gatcctgcca tcaaatatcg aaaatccgcc
     2041 actcgacctt cgcctgatca ccaaggcgag ccattcccaa taaaacggta taggcggttt
     2101 catccgcttg aaaggtcaat tgcagctgtc ctcgtaaatc gttgtagtca tctggggcgg
     2161 ctacttgata aaaaagatga aactgcgcat gcaaatattc caaaggctga tctttaaagg
     2221 ctgcaaaata agcctgaact ttttcccgct gcaagctgtc agccaccaaa aaattttcca
     2281 gttgttgttt tggaacggct gctaacaagg atcggcaaac cgcctcaatt ttcaacgcat
     2341 cccaatgtcg cgcaattaaa taatggatgg gtgcctcttg attgtagtcc cacaggttgc
     2401 tcattttttt caccactttc ttctattata taggaatcac aaaatcggca gcggcgtcag
     2461 gcggcgttct tttccttgaa tgactagcaa cttttctacc tgacagtctg ccaacagctg
     2521 actcattttt ttaaagccca gctgataatc ttcggcaaca tgagcatccg agcctaaggt
     2581 aaagagcttt ccgccgacac tttgatacaa aggaacggca taacggtaaa ggtcttcgtt
     2641 gccgtatttc aagaagctct tggcatttaa ctccatcgcc aattctcgtt caacaacctt
     2701 tttgaaaatc gttagcagct gagcctcaaa atgctccgcg aattcttccg ctgtgaaggt
     2761 gaagcggcgt aatccataat caaagtgagt caaaatatct ccttctggaa aatccgtcaa
     2821 gacctgcaac atctgatcaa aatattcctt ggtagtttga atgggatctt tggttaaaac
     2881 cacatcgtcc atataatcaa agcggccatt ttgatgaata cttaccagct ttaaatcata
     2941 aggatgctga gcgaggtaat ccacgatccg ctcttcttgt cctggaacaa cgccaatctc
     3001 gatacctcgc agaaatttgg tggaataggt ttcttttaaa gagtccagtt tgtttatata
     3061 ggctggataa tccggaatgt catcttttcc ggtagatgga ttgtttaaat caaagtgatc
     3121 cgtagaaacg aaaaactccg gctgataggc taaatagttt tcaaattttt cctctgaatc
     3181 aaaggaaaaa tacgtgtgga gatgctgatc ataatactgc atagggagcc tccttttttc
     3241 tactatcata ccgcaaaagc tttagaaggt taacgggaat tcgtcgaaat ttacaaacca
     3301 tatacaacct ttacattgtt catgaaacct tttcaagaaa ctgattctct cattacaaag
     3361 ctttgttaga atggaattat cgaaagggga gaagaatgaa tgaaaatgaa tgaaaaaatt
     3421 cgtaacatga ctatcggttt attttgtgca gcaacagtaa gtatcctgct gacaggctgc
     3481 ggctcaggtg aagcaacgaa gcaatcggct gcggcagacg actctttaga tacgattata
     3541 gaaaaagcca aggaagaagg agagattgcc agcttgggaa tgccggatac ttgggcaaat
     3601 tgggtaggca cttggaacga ccttgaaaaa gaatacggct taagtcacgt ggatacggat
     3661 atgtccagtg cagaagaatt ggctaagttt gaatcagaag gaaaaaatgg aaccgccgat
     3721 attggtgatg tcggtatcag cttcggtccg ttggctgaaa gcaaagagtt aaccttacct
     3781 tataagacca actattggga ggaaattccg gattgggcaa aggatgatga tggggattgg
     3841 ttgttaagct atacgggaac catttccttt atcaccgata aagagaatgt gaagcaagca
     3901 ccgactagct ggaaggagct gcaagagggt gactatcaag ttagcgtggg ggatgtgacg
     3961 attgccaacc aagcacaatt tgctgtattg gctgcggcga ttgccaacgg aggtgacgaa
     4021 aaggatattc agccaggtct gaattactac aaaaaaatgg cagaaaaagg ccgtttgtcc
     4081 accgttgatg ccagcgtagc aaatcttgaa aaaggtgaaa tcgatgtggc agttgtttgg
     4141 gacttcaatg gactaaatta tcgtgaccaa attgactccg agcgttttga ggtaactgtg
     4201 ccaacagacg gctccatcat tagcgggtat acaaccttaa tcaataaaaa tgcccctcat
     4261 cccaatgcgg caaagctagc acgggaatat attttatctg atgaagggca aatcaatttg
     4321 gcaaagggct atgcgcgacc tattcgcaag gtagagctgc ctaaagaagt agctgaaaaa
     4381 ttactgccgg aggatgctta tgaagcggcg gtgccagttg aagacaatga agcttggaac
     4441 aagacctctg tagagcttcc agagctttgg caggcggagg tactgaccta tgcaaagtag
     4501 acttgacaaa ctgattgtag ttatactaga cggctgtcgc tacgacacag ccgtcgccca
     4561 gcttgggttc atgaatcatt tagttgagca ccagcaagca agcttattga aggtaaaaag
     4621 tgagctgccc agcaattcac gtccgttata cgaagtactg atgaccggcg ttccagccta
     4681 tgaaaatggc atctatagta attaccatgc tcagcgatcc aaagagtggt cgctgtttga
     4741 attagttaag gaagctggcg gaaaaacagg agccgccgct taccactggt acagcgagtt
     4801 gtacaatcaa gccccctata atcgtttggt tgatcgcatc cagcatcagg aggagcgact
     4861 gattcagcat ggcatctttt atagtgagga cacttaccct gattcgcatt tatttgcgga
     4921 tgcccattat ttagtgaatc agtatcagcc agactttttg gtcgtacatt cgatgaatat
     4981 tgatgatgtc ggtcacaaat ttaccgccga ttctcatgaa tatgcagcag cggttaatca
     5041 ggcggacaga ttattggcta tttaccttcc tgaatggctg caaaaaggtt atcaagtcgt
     5101 ggtgactgcg gatcacggga tggatgcgca cggactgcat ggcggcagct tggctgcaca
     5161 tcgtgaagtt cctttttatt tgatttcaaa taaacgccaa aagctgaagg aacaaactgt
     5221 accacagctg ctggcggctc cattgttttg ctggttatta ggaattgcac cgtcagagaa
     5281 aatgcaggac attcaagaat tgatgagggg atgaaagaga tgaaaaaacg cttggttctg
     5341 gttcctgcct cctttatctt ctgtgcattt ttactgctgc cgctgctttt tatggttctg
     5401 tgcagcttgc acagtgattt aactaaccag tggacattgg ccaattacag tgatattttc
     5461 agcaacaaat attacacaca ggcctttgtt aacagcgtac tgattgctgt cagctccagt
     5521 gtgattgggt taatcgggac gtttatttta tgtttatgct taatgaatct atcgactaaa
     5581 ttgcaggaga aaatcacctt tgtttccaat ttggcagcta attttgctgg gattccgctg
     5641 gcattctctt ttattatttt gttggggaat gtaggtgtgc tgaaaatgat tttgccattt
     5701 ctagcggact tcaatttgta ttcttggtgg ggattgacca tcacgtatac ctatttccaa
     5761 attccgttag gggtcttgtt tttgtatccc agcatgaagg aagtgaaaaa ggaatggctg
     5821 gagtccgcac aattgctggg cgcttcatca gcgtatgctt ggttcaaggt ggctttgcct
     5881 tttttgcgac catcgattgc cagcaccttt gtcatcttat ttgctaatgg catggggacc
     5941 tatgaaacag cgtatgccct gaccagcagt aatgtaaatc ttttaacgat tcgaattgcg
     6001 gctttagtgt cgggagatgt gttcgccaag cctaatttag gcagtgcgtt ggcagtcgct
     6061 ttaggtgtcc tgctggtaac ggcaatgttg attattcaaa agaaaggcaa ggtggtgcag
     6121 acgtgaaaaa gttaataaaa ccgattttgt tctgctttgt cctgtattac ttgctgccga
     6181 ttattggaac gattttgtat gccagctcca ctaaatggtc aaaatcgctt ttacctagtg
     6241 attttacctt gcaatggttc caacagctgc tgacggacag ggaatttatt gctgcggtgg
     6301 gtcgctcact tttattagca ggtgtggtct tggtgacgat tctgttgttg atgattccca
     6361 ccattatttg gattcatttg tatttccctc gtttgaatcg ttggctggaa aagctgctcc
     6421 tgctgccgta tgctttacca ggagtgattt tggtaacggc tttgcttcga acgtatgctg
     6481 aaactggtat cccaatgttt gtagtgttgg taggtgcatt gtttattacg gcgttgccga
     6541 ttgtctatct tagtcttaac aaccagatgc gcttaattaa tctgaaggaa ttagtggatg
     6601 cagcagaaac cttgggcgcc ccgatgtcta cgattattat ccaggtgctg tttccaaaca
     6661 tccgtatcgg tgtgacccta gtttctctga tgattttttc ttcggttttt ggggagtaca
     6721 tgctgacgaa cctgttgatt ggcggtcgat ttcagacggt acgcatctat atgctgcggc
     6781 ggatgaacga aaacggccat ttggctagcg ctgtgatggt tttgtatttg ctgtttatgg
     6841 tgggaattgc tttaactacc tttatgctga cttatcgaca aaagcaacag ctgtccctta
     6901 agcagacgag ggaagagaag cagtcgaaaa agaagccctt aaaggagttg tgacggaaaa
     6961 tgtttttaga aatacatgat ctcaatctgt tttttgatga aaaacagatt ttagctattg
     7021 atgaaattca gattgaaaag ggtgagctgg ttactttact aggtcccagc gggtgcggaa
     7081 agtctacgtt gctgcgctgt atcacgggat tggaacaacc aaagagcggt gagattgtgc
     7141 tggacaatga gaatattgcc gataagccta cgaaggatcg caatatcggc tttgtttttc
     7201 aacagtatgc attgtttcca actatgactg tttttgaaaa tgtagccttt ggtttgaagg
     7261 taaagaagct gagtcctgag gtgattcagg aaaaggtttt tgagatgcta tcattggtgg
     7321 atatgacgga gcaggcagat aaaaatgtcc agtttctttc tggcggtcaa cgacaacggg
     7381 tggcactggc tcgctcattg gtcactgaac cgaaagtcct gctgctggat gagccattaa
     7441 gtgctttgga tgcccgaatt agaaaacagc tgcagcggga tttgcgcgct atccagcaat
     7501 cgctagggat gacgatgatt tttgtgactc atgaccaaga agaggcgatg cgaatcagtg
     7561 atcggatttt tgtgatggaa gctgggcggg tggctcaggt ttccacctcg aaggaattgt
     7621 atcagaatcc tcagagccga tttgttgcgg aatttatcgg caactataat cgtttcgaat
     7681 ggtatgagct gaatcagtat acccacgagt tttccgctaa gggcaatcgc tgcatttatt
     7741 atttaagacc ggagctgatt caatttgaac cggttcctca gggggttaag attccggttc
     7801 tatggaagga atcatttatt ttaggcaaca ttcaacggta tgtttttcaa acgctggagg
     7861 gcaaagaaat tttagtggat cggttgaacg atcgggaaga gaaaattagc gatgccttat
     7921 acattcagct gaaagatatt ctagcaatcc ctgtggaaat tgatactcag cattttgcac
     7981 aacaaagcta gtgtatagtc ggcaaacatt tctggctctt tgtcaaatag gactgataga
     8041 gtgatataag aacatctaag gtagaattga ttttctgctt tagatgtttt tttatgttta
     8101 ataagtgatc agatgattga ttaagaaaat tctggttctc atcctaaccc gtaagctatt
     8161 cgtttcaatg ctttgatgtg ggtatgcaga ttttctattt tgccatttga gtaaggcaac
     8221 tctaaggcta aacgaatgcc ggattcatag tttataaacg ctttttttat atgatgcagt
     8281 tctcttggaa ccgacttggg cattgttttt acaagctgaa agaagatgtc cgcttctttg
     8341 tcataaaaag cttgaagaat ttcttgatag taagcataag ctattcgtag ttcatcagaa
     8401 gtagaaagta gacgttctac gacttcactt tcggttaaat aggcccaacc aaaattacgc
     8461 cattttgtcc atttatcata aacaagcgtg tcctgtcgtt tgatcagaag tcgagataaa
     8521 gccttcactt gtcgatagtg tttggcttgt attggatcgt ttcggtcgaa gcttttcatg
     8581 actcatagtc aaatatcctt aaaactgcga atgaggtgct tgacgatgtg aaagcgatcc
     8641 acaacgagtt tggcatgagg aaagcactct tttatcagca aagcataaga ggcgttcata
     8701 tctgttacga gtatttttac gtttaaacgt gcacgtcgtt caaattgcat aaaataagaa
     8761 atgagatctt tcttcttacg tgaaggaaga atatccagta atttcccagt atcgccatcc
     8821 atacaagaaa aagaataggg accgacttga tttttcaatg aacgaaattc atctaccaat
     8881 gaacagcgag gcaaccaatt tattttcacg tttgtcatgg ggagcagaga agcgagtgtt
     8941 cgcgaaacgg ttgaccaaga aacgccgcaa tgtttagaaa tcgtcgtcat tgcttgtttt
     9001 tcagtcaatt cctccaatat tttataatgt acttgtttgg cgataaaaca gtttttttca
     9061 acaatgtagg tattcgccgt aaaataggtg tgacagccac gacagaggta ccgttgcttt
     9121 gccaaagcaa gataagtccg ttgattcgca cacttattga gaaggaccag cgattttttc
     9181 tttccgttct taacgatcag cccatgttga ttcacctgtt tacactgagg acagggctga
     9241 atcgttttaa tgagcgtggc agaaacaacg atactgttga ttgaatgtat ttttttcttc
     9301 tcgacatgag tgatttgaat gtttccatct ttgattccta gtaatagttt gataagatga
     9361 agatcaggca tacagaacgc tcctttgatt gtgttgtggt gattcaattc taacggagct
     9421 tctgtatgtt ttgttttttt atgctcaaaa ggactgatag aatgatttac tatcagtcct
     9481 agaaattata gcgccacatt tctttcacct ttgacttttc agacggcagt ttttatgctg
     9541 atactgtaga gagagcagga cacagctttt gcttgttttg ggtaaggggg aaagaagatg
     9601 accaaagcac ttatttttga ttttgatggg acgatgattg acagtgaatg gatgttttac
     9661 gagagtattc atcactattt actgaagcct atttactgga gcagttcggc tataaaattc
     9721 cttttgcaga ctatcaacaa ggtgttggga cggcagcaga actgtccttt tatgaaggga
     9781 taagtcaagc ggttgggaga ccagtggatg ctgaacattt tgagcagcgg atcagcagca
     9841 ctggaaaatc ggagtagtct caattctgaa ctgcgggagc tagactattt ttttgaagca
     9901 catccgatga ttaaggaaaa aattgattcc tttgtgacga tcgatgatct tcaggtcggc
     9961 aaaccagatc cagaaggtta tttgttgtgc ttgaaacggc tgagggttga acaggctgtt
    10021 gtagttgagg atagtccagt cggtgcccaa gcagcaccgc atatgacagt tgatgtacaa
    10081 gcgttggatg cagatttttt cgcctttagc gggcataaaa tgtgcgggcc gacaggaatc
    10141 ggtgtattat atggcaaacg tcaattgtta gaacaaatgg agccagtcga attcggcggt
    10201 gaaatgatag attttgtcaa tctttacgat agttcatgga aagagctgcc atggaaattt
    10261 gaagctggta ctccgaacat tgcaggagca atcgattact tgaatgagat tggtatggag
    10321 aatattcatc gttatgagca agaactggtt gcctacgttt tacctaaatt acaagcgatc
    10381 ggcggtatca ccatttacgg accgcaagat cctgaacatc atactggcgt ggttgctttt
    10441 aatttggatg gcatccatcc ccgtgatgtg gcgactgctc tggatatgga aggtgtcgct
    10501 gtacgttcag gacaccattg tgcacaacgg ttcatcgacg atttctgttt gactaattgg
    10561 tcgtttaaac agtcgatgat aatgccgttt gagcggctcc aatgtcgtgg aatctttgag
    10621 taagagtttc caatatcgct tgagccgacg gtacttcttc tgattttcag attacgtgat
    10681 tgattttcgt acagttctat gagaatcgcc tgtttcagtt cctttgttag ttgatgatgt
    10741 tcatcaacta agtccgtttt cgcagaaaag gtctgcccac aatcatagca tagaaaacgg
    10801 gtacgtttta attctaaaat agtcaacttt tctttgactt tcaacatctg agtacgtgtc
    10861 gtataggaac cgttgcggat aatacgaatc gatccacagt gttcacaatg ggaaggtgag
    10921 tcatccaatc tccctttaat aaactgcgct tgaacgcctt tatattcttt ttcaatcagc
    10981 cattcttcat caaaaaataa atgtttatct gttaatccga gtaattttct agtatgatta
    11041 tccatagagc gactcctttg gtagggtgat gttttggtcg actaaatttt accaaaagtg
    11101 agtctctttt tgagttgaaa cgaagaaaaa gtgttagtag aaaatcgcct tcgattttcc
    11161 aacaacacaa aatattatag aagccttttt tctaaaggat ttcaaggtgt ctgtttgcat
    11221 ttattaaaaa aaagaaaaag tattggcaac agaaggcgtc accaacacta gatattatag
    11281 agccataata tgctgtataa tatctagtgt ataggcttaa gtggaggtat atatgaaaat
    11341 attaactgtt gaagatgata atatgataag agagggaata agtgaatatc tttcagaatt
    11401 tggatatact gttattcaag ctaaagatgg aagagaagcc ttgtcaaaat ttaatagcga
    11461 tataaatttg gttatcttag acattcagat accttttata aatggtttag aagtgttgaa
    11521 agaaattaga aagaaaagca atttaccaat tctaatcttg actgcattta gtgatgaaga
    11581 atataaaatt gatgcattta ctaatttagt agacggatat gtagaaaagc cattttcatt
    11641 gccggtctta aaagctagaa tagattcttt aattaagaag aattatggac atttagagaa
    11701 gtttgaatat aaggatctat cggttaattt taatagctat acagctaaaa taaatggtga
    11761 agaaatagat gtaaatgcaa aagaactaga agtattaaaa tgtttattgg ataataatgg
    11821 acaagtgtta acaagaatgc aaattattga ttatgtatgg aaagat
//
LOCUS       DANKMN010000089        11803 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000089 DANKMN010000000
VERSION     DANKMN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..195)
                     /locus_tag="PW224_002509"
     CDS             complement(<1..195)
                     /locus_tag="PW224_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909141.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="PW224_002510"
     CDS             complement(211..825)
                     /locus_tag="PW224_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL2909142.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="PW224_002511"
     CDS             complement(972..1655)
                     /locus_tag="PW224_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2909143.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="PW224_002512"
     CDS             complement(1764..2222)
                     /locus_tag="PW224_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL2909144.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="PW224_002513"
     CDS             complement(2306..4069)
                     /locus_tag="PW224_002513"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL2909145.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="PW224_002514"
     CDS             complement(4290..5750)
                     /locus_tag="PW224_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909146.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="PW224_002515"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="PW224_002515"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL2909147.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="PW224_002516"
     CDS             complement(6907..8334)
                     /locus_tag="PW224_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2909148.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="PW224_002517"
     CDS             complement(8365..9579)
                     /locus_tag="PW224_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL2909149.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9920..10162)
                     /locus_tag="PW224_002518"
     CDS             complement(9920..10162)
                     /locus_tag="PW224_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2909150.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10194..10751)
                     /locus_tag="PW224_002519"
     CDS             complement(10194..10751)
                     /locus_tag="PW224_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2909151.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10764..10952)
                     /locus_tag="PW224_002520"
     CDS             complement(10764..10952)
                     /locus_tag="PW224_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2909152.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10965..11531)
                     /gene="amaP"
                     /locus_tag="PW224_002521"
     CDS             complement(10965..11531)
                     /gene="amaP"
                     /locus_tag="PW224_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2909153.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3461 a         2447 c         1933 g         3962 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca tataaaatat
     9841 ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc atttcaacat
     9901 aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact aatactaaaa
     9961 tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt ccccaagttc
    10021 ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt gcgatgatcc
    10081 ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata ccgccgacga
    10141 ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac ttcttattgt
    10201 acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc agattttgct
    10261 ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt tactttgtct
    10321 tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc aacattcaca
    10381 ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat caattctttg
    10441 atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc tagatccaca
    10501 gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc tgtgttgact
    10561 aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc atctacatct
    10621 ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa ttcaccattc
    10681 atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt attcatgttt
    10741 ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat tcctgactgt
    10801 ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa caatgtcttg
    10861 aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc aaagataata
    10921 gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc cactcgagag
    10981 tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac tgccactttg
    11041 accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg catcaataca
    11101 cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg gatatggatt
    11161 cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc ttcgtgtaaa
    11221 tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc atcttcccgt
    11281 ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa gatgataata
    11341 gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta gtaattcgtt
    11401 aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc attgatcaac
    11461 gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat tatttttaac
    11521 cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa ctctaccgac
    11581 aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg ctaccaaaca
    11641 ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa ttgcttactt
    11701 attctctatt tacttttgat tgctggctat acttaaagga tattattcaa gattaaaaat
    11761 tgcaaagatt atgctcagtt attattacaa gattctattt att
//
LOCUS       DANKMN010000090        11665 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000090 DANKMN010000000
VERSION     DANKMN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(132..509)
                     /locus_tag="PW224_002522"
     CDS             complement(132..509)
                     /locus_tag="PW224_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909154.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /locus_tag="PW224_002523"
     CDS             765..1493
                     /locus_tag="PW224_002523"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL2909155.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="PW224_002524"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="PW224_002524"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL2909156.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="PW224_002525"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="PW224_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL2909157.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="PW224_002526"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="PW224_002526"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL2909158.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /locus_tag="PW224_002527"
     CDS             4628..6067
                     /locus_tag="PW224_002527"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL2909159.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /locus_tag="PW224_002528"
     CDS             6090..7142
                     /locus_tag="PW224_002528"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL2909160.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="PW224_002529"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="PW224_002529"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL2909161.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="PW224_002530"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="PW224_002530"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL2909162.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="PW224_002531"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="PW224_002531"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL2909163.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="PW224_002532"
     CDS             10661..11575
                     /locus_tag="PW224_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2909164.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
BASE COUNT         3834 a         1972 c         2733 g         3126 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc ttttt
//
LOCUS       DANKMN010000091        11128 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000091 DANKMN010000000
VERSION     DANKMN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="PW224_002533"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="PW224_002533"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL2909165.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="PW224_002534"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="PW224_002534"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL2909166.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /locus_tag="PW224_002535"
     CDS             2139..3020
                     /locus_tag="PW224_002535"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL2909167.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /locus_tag="PW224_002536"
     CDS             3017..3733
                     /locus_tag="PW224_002536"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL2909168.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="PW224_002537"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="PW224_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL2909169.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="PW224_002538"
     CDS             4450..5451
                     /locus_tag="PW224_002538"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL2909170.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="PW224_002539"
     CDS             5448..6809
                     /locus_tag="PW224_002539"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL2909171.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="PW224_002540"
     CDS             6769..7920
                     /locus_tag="PW224_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL2909172.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="PW224_002541"
     CDS             7895..9064
                     /locus_tag="PW224_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2909173.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="PW224_002542"
     CDS             9239..10618
                     /locus_tag="PW224_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2909174.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10899..11128
                     /locus_tag="PW224_002543"
                     /pseudo
     CDS             10899..11128
                     /locus_tag="PW224_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3702 a         1945 c         2494 g         2987 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10861 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10921 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    10981 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11041 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11101 gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DANKMN010000092        10088 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000092 DANKMN010000000
VERSION     DANKMN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(114..2234)
                     /locus_tag="PW224_002544"
     CDS             complement(114..2234)
                     /locus_tag="PW224_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2909175.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2464..3642
                     /locus_tag="PW224_002545"
     CDS             2464..3642
                     /locus_tag="PW224_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL2909176.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3658..4416
                     /gene="aroD"
                     /locus_tag="PW224_002546"
     CDS             3658..4416
                     /gene="aroD"
                     /locus_tag="PW224_002546"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL2909177.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4439..4924
                     /gene="ybaK"
                     /locus_tag="PW224_002547"
     CDS             4439..4924
                     /gene="ybaK"
                     /locus_tag="PW224_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL2909178.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4995..6248)
                     /locus_tag="PW224_002548"
     CDS             complement(4995..6248)
                     /locus_tag="PW224_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2909179.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6365..7714
                     /gene="gdhA"
                     /locus_tag="PW224_002549"
     CDS             6365..7714
                     /gene="gdhA"
                     /locus_tag="PW224_002549"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL2909180.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7826..9172
                     /locus_tag="PW224_002550"
     CDS             7826..9172
                     /locus_tag="PW224_002550"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL2909181.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9480..9866)
                     /locus_tag="PW224_002551"
     CDS             complement(9480..9866)
                     /locus_tag="PW224_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2909182.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9915..>10088)
                     /locus_tag="PW224_002552"
     CDS             complement(9915..>10088)
                     /locus_tag="PW224_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2909183.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3218 a         1839 c         2054 g         2977 t
ORIGIN      
        1 aaaatctgag ccagaatgag gttcattctg acccagattt gattttgtac aacccatcag
       61 tccttattga caaagagcct aattttgtcg ctgctccttt taaatttatt ttatcattcg
      121 gaagaacttg tggtcgttcc gtgaagttct tggtacgttt gcgttttata aagatcaaca
      181 gttgattgtc cgttgtgttc actaaagaca ctagttgact tgtcaccaag agatttttca
      241 gcttctttta atttctctaa gccattcttg tagtcataat cactggtatc aattgggtct
      301 aatccactat tcgtatagaa ccgaagaaga tcaccattat tgatttggtc agaaacagct
      361 aactgtttat caactttttc tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt
      421 tcagtaatag cttcaccagt ttcgttacta tagatcgtac caccgtagta agtatatttt
      481 ggtgtaacaa aatcaccatc acggaaagaa acaagctgtt catgatcttt cgagaataga
      541 tcttgtccta actgaatata attttttgta tctacaccta gtaagtgaag taatgtaggc
      601 aaggcatcaa cttgtccgcc gtaagtgtga ttgattccgc cctgtgtttg tccagggata
      661 tggatcatat aagggacacg ctgcatttgt gcattgtcgt agtttgtcca agtagaactt
      721 gtttttccaa ctaattcagc tagattttta tttcttgaat tcgagacacc gtaatggtca
      781 ccgtaaagga cgatgactga attatcatac aagccgcttt ctttcaaata attgaagaat
      841 tcttgtacgg cggtatctaa ataattggct gtcgcaaagt aaccgttgat cgtttcatca
      901 tttgtattgg ctaacgggaa tcctgcttcg tcattggtga attttgaata aggataatgg
      961 ttagatacag caatgaattt tgaataaaat ggttgttgca gacgttccaa gtattgtacg
     1021 gattgttgga aaaatggttt atcatgcaac ccgtattgga acgaattgtc atcattaaca
     1081 tcataataag aagaatcaaa gaaataatca tatcctaaat tcttgtatgt ctcgtttcga
     1141 ttccagaatg taccagcgtt cccatggaat accgcactag tgtaaccttg tgtttgactt
     1201 aaaatatcag gtgcagcttg gaaagtattt tttccgccta attgtgtgaa caaggagcct
     1261 tgattcaagc caaacaatga attttccatc agcgtttccg catcgcttgt ttttcctgct
     1321 tttacttgat ggaaaaagtt gtcaaaactg aacgtgctgt tgctgtggta aagactattg
     1381 atgaaaggtg taacttcatg ctggagacca ttctcatctg tcaatttata atcgatcagg
     1441 aattgctgga agctttctaa atggatataa atcacatttt ttcctctagc gagcccgaac
     1501 aaatcgtcat ttggtgctgc ataatgttct tttacataat tttctacttc tttcatatca
     1561 ttgggacttg cttctgcccg cacttggttt gtctggtaag tttgaacacc gtcataaacc
     1621 atgaaagcat tcaagccaag atatttgacc aaataatcac gagagaatgt tcgagtcagc
     1681 aattctggac gatcagcttc cgccataaaa agatttcctg agaaaatcat aactgctaat
     1741 gtagagatcg caaaagccat tcttgctcgg atcgggcgag ggtccaactt gatttttttt
     1801 gcaatcagca gaacagtcaa gataataaaa tcgatccaat agatcacatc atatgggcga
     1861 aacagtcgaa tggcactttc tcccagacca ctcgctactt tcccagcacc taaaatcgtg
     1921 ttgatcgtaa tgaagtccgt gaattctcga taataagtga cattcgaaaa aacaagcaat
     1981 gtcaaaagaa aataaatgac catcatcgtg atataagcaa ctctagggcg tctgatataa
     2041 agagcaattc caagtaataa caatgtagtt gcaatagggt tgataaacaa gataaagtat
     2101 tgaatgccac tttcgattcc gagatggaaa tcaactaaat aggcaaaaat cgttttcaac
     2161 caaagtaaaa cgactagtaa agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt
     2221 tttatacatt tcaagataat cttcctttct gcacttctta atcacagaag tgtcctgaaa
     2281 ccatgttaca cagctcactt attctcattt attttactag tatgacaata tagtgtcaat
     2341 tgaaaccaga agtttatgct gtttcattaa ggttttcttt aagcttcatg aaaaaatgag
     2401 cagtaagaca agtcctatgt tttttgttat acttgggaag agaaagctaa agcaggtgaa
     2461 ggaatgaaag taaaagtaaa aaaaagagca agcaatcgat tgaaaaaagg atatccactg
     2521 atccaaaaag aagaccttgc tcatccccaa aaagaaacca cgcaatgggt ggaattagtt
     2581 gattcgcaaa accaatttat cgcaaaagga tatctaggaa agcagaataa gggaatcggt
     2641 tgggtattga gccagcagaa catcccattt gatcaatctt tttttgaaga attttttgtg
     2701 aagtcaaaag aaaaaagaac tttctttttt tcagatgatt ctactaccgc ttttcgtata
     2761 ttcaatggag aaggagacgg gatcggcggt ctgattgttg attattatgc aggttatgct
     2821 gttttttctt ggtataacga aacgttatat gcacatcgtg aattattgat tcaagctttt
     2881 cgtgaaatct acccggaaat cataggaggc tatgagaaaa ttcgatttga atcagtaaat
     2941 ctgcctgaga gccagttcat ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa
     3001 aacggagtaa aatttgccgt ttatttaaat gaaggattaa tgactgggat ctttttagac
     3061 caaaaagaag tacggggatc attagcagaa ggtttcgctt ctgggaaaac agttttgaac
     3121 atgtttagtt ataccggcgc cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca
     3181 acaagtgtcg atctagcaaa acgcagttta aaaaaaacac aggaacagtt tgaagtgaat
     3241 ggtttggatg tcgatcagca aaaaatcatt gttatggacg tattcgagta tttcaaatac
     3301 gccaaaagaa aacaattttt atatgacatg attgtattgg atccaccaag ttttgccaga
     3361 aataagaaaa aagtctttcg cgtagcaaaa gattatgggg aactggtcaa ggatagtctg
     3421 gatattttag cagaagacgg gatacttatt gcgtctacaa atgctgcgaa tgtctcgata
     3481 gaacaattcc aaacaatgat tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa
     3541 cttgctttat atcgtctgcc aaaagatttc cagacgacgg ataccttttt agaaggcaat
     3601 tatctgaaag tgtttgttta tcaaattaaa aataaccatt agaaggagcg atgcagaatg
     3661 gttcgggtaa agggattgaa tataggaagc ggtagaccaa agatttgtgc gcctcttatc
     3721 ggggccgtcc gagaggaagt attacgtgaa gcagttcttg cacgtgaagc aggggccgat
     3781 ttagtcgaat ggcgagtcga tcattatcgt gaagtttttt cttttgatga agtagcagaa
     3841 acattggttc ttatccatga gatgcttgat ggactgccgt tattatttac gtttcgtacc
     3901 ttagctgaag gcggagaaca agacatatcg atccgtcagt atcgcgacct ttatgaacat
     3961 gtgatacaca caggattagt agatatggtc gatatcgagt tgttcaaagt agaaagtcta
     4021 ggaaaaagac tgatagagga aatcaagtct ttaaacattc ctttaattat cagtagtcat
     4081 gattttaaag aaacaccagc agaccctgtc ttgctttacc gattgaatgt tatggaacat
     4141 tttggagccg atatcggaaa actggcagta actcccaata atgaacgaga tgttttgcgg
     4201 ttgatggagt tgactagacg ggcaaatgct tttgtctcca tgcctattat tacgatgtcc
     4261 atgggaaatc ttggtaaaat ttctcgtctg gttggcggaa cgacgggttc cgttatgaca
     4321 tttggttcct taagcagcga ttctacttct gctccaggac aattaccggt caaagcattg
     4381 aagcaaatta ttcaattatt aaatgaaccg ttgtagaaca agtttgaggt gatatttttt
     4441 ggcaaagaaa aaaatagtaa aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc
     4501 ctatacagag cacgaatatg agtgggacga aagccaccta agtgcgtcat cggtcgctga
     4561 acagcttcca gaaagccaat ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac
     4621 ggggcctctt gttgctgtga taccgggaga agccgaattg aatctaaaaa aattagcaaa
     4681 agtttcaggg aataaaaaag tagaaatgct ccatttaaaa gatttggaag caacgaccgg
     4741 atacatacgt ggtggctgct cgcctatagg tatgaaaaaa ctatttccta cttatttaga
     4801 tcaaattgca gaaagctatg aacaaatcat tgtctcggct ggcagaagag ggctacagat
     4861 ggagttagca cctcaagata tctgtgcatt aacgagcgga caatttgcag atataaaaca
     4921 ataaatcata cgtataaaag aaagaaggag gccgggatgt tgtttgtcca gtctccttct
     4981 ttctttttta tacattatgc tctttgcccg gcattcattc ctgctacatg ccctgtagcg
     5041 aaagccgctg tgatattaaa gccgccagta taaccattga catccagtaa ttctcccgca
     5101 aaatacagac catcaatgat tttactctcc aatgttttag gataaatttc ctttagagaa
     5161 acccctccgc cagttacaaa tgatttctca ataggaaaag ttttgttgat tgatagctta
     5221 aactctttgc atagttgaac aaaatcttga atttgttttt gagaggcttg ttgtcctgtc
     5281 agatgatcca tttcaagccg ctcaagaaag aaaactaaca atcgctcagg caatatacca
     5341 tgccaagcat tgaccaagtt ctttttggtt gctttacttt tttctgtcaa tatatgaata
     5401 agctcttgtt tggtttgtgt agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt
     5461 ttctctaatt ctttattgac aaaacttgaa caacgcagtg cagccggacc agaaataccg
     5521 aaatgggtga aaagtaaatc catcgtatgt tcagtcaata cacgaccttt tttatttaaa
     5581 acgcgtaaag tgatgtcttg taaagaaagc ccttgaagtg ttttctcctg aatatacggt
     5641 tcatccgata tcaaggggga ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc
     5701 ttcacgatct tgtacccatc tccagtagaa ccagtagatg gataagtccg tccaccagtt
     5761 gttaagataa tggcaggagc atggaactct tcgaattccg tgcgtatacc gtaaatttta
     5821 tgttctttgt ggatcaattt ctctactcga gtagagaata gaagcgtcac atctagttct
     5881 ttcagacgat tgagcaatgc ttcgataatc gtttttgatt tatttgttac aggaaacatc
     5941 cgtccatgat cctcttcttt taaatgtacg ccttgtgatt caaagaagtt catgatatcg
     6001 aaattattcc attgcgcaaa cgtactatat aaaaactttc catttccagg aatatgtgca
     6061 atcaaatcat caacaggacg attgttcgta acattgcacc gaccaccacc tgtcatcagt
     6121 agtttttttc ctgctttttt gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct
     6181 gcactgatcg ctgccatcat cccgcttgtt ccagcgccga ctacgatcac atcatacagt
     6241 ttttccattc cctcacctca aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa
     6301 atgttttgag ccaaaagaga aaatgagcta aaatgaggga agataagcaa aggagcgtgg
     6361 aacgatgaca aacgcaacag aatatgttca agcaattcaa gaaaagctac atcaaaagga
     6421 tcaagggcaa gaagaatttt tacaagcaat cgatgaattc atgcccacgg ttatgtcttt
     6481 tttggacact catcccgaat atatcgaaaa gaatatctta gaacttttaa cggaaccaga
     6541 aagagttatt caatttcgag tgccttggca agatgatgaa ggaaactggc gagtaaatag
     6601 agggtaccgt atccaataca actcagctat cggcccttat aaaggtggat tgcgcttcca
     6661 tcctagtgtc aatctaagta tcttgaaatt tttggctttt gaacagattt tcaagaatag
     6721 tttaaccggt ctgccaatcg gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa
     6781 atctgataat gagatcatgc gtttttgtca aagtttcatg ctggaattgt caaaacatat
     6841 cggcccatcg attgatgttc ctgcgggaga tatcggtgta ggagctagag aaatcggcta
     6901 tttgttcggc gaatataaac gattgaaaaa atatgatact ggtgtgttga caggtaaacc
     6961 tttggatttt tggggaagta agatacgtac ggaagctact ggttatggat tagtgtatta
     7021 tgtcaaacat ctattgaacg aagaaaaaga ttcatttaat caaaaaacag tttttgtttc
     7081 cggaagcggg aatgtagcca tttatgcaat cgaaaaagtg caagagctag gcgggaaagt
     7141 tgttacatgt tcagattcta gcgggtacat ctatgatcca gaaggaatag atgtgaaact
     7201 attgaaagaa gtcaaagaaa tcaatagaca acgtttgacg gaatacgcaa accaacgtcc
     7261 ttctgctatt tatcatgctg gagaatctgt ttggacattg aaagaaaaag cggatatcgc
     7321 tttgccatgt gctactcaaa atgaaataga cgaaagcttg gccgaaatct tagtagaaaa
     7381 tgggatcaaa atcgtagcag aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt
     7441 tcttcatgaa gcaggcgtgt ggtattgtcc aggaaaagct gctaatgctg gaggagtagc
     7501 agtatctgct ttagaaatga gtcagaacgt ccagcgattg acttgggaaa gagagcaagt
     7561 ggatgagcag ctggatcaaa tcatggaaac gatttaccat acgtgtcgcc aaacagcagc
     7621 cgagtacggc gacgaaaaaa atctgcttct tggagcaaat gttgctggat ttgaaaaagt
     7681 tgccaaagca atggcagcag aaggtttagt ttgaaaaatg aaactttctt ggaaatattg
     7741 tcagcatttt gtttatttgc ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag
     7801 agtaaaccta taaggaggag aacaaatggc acacattcat tttgactatt caaaagtagc
     7861 accgtttgtc aacgaacaag agttagggta catgcaaagc caagtaacag cagcccacaa
     7921 cgaattacgg gaaggtactg gcgcaggaag cgacttccgc ggctggatcg atttaccaac
     7981 gaactatgac aaagaagaat ttgcacgtat caaagaagca gctaaaaaaa tccaatctga
     8041 ttctgaagta ttagttgtta tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat
     8101 cgacttcttg aatcacacat tcttcaattt attagatagc aacacaagaa aagcaccaca
     8161 aatcttcttt gccggaaatt caatcagctc aacatatatt gctgacttga tcgaagtaat
     8221 cggtgatcgc gacttttcag taaacgttat ttcaaaatct ggaacaacta ctgaaccagc
     8281 gattgcgttc cgtgtattca aagagcttct tgttaagaaa tatggacaag aagaagcaaa
     8341 caaacgtatc tacgctacaa cagataaagc aaaaggtgca gttaaagtag aagcagatgc
     8401 agaaggttgg gaaactttcg tgattcctga tgacatcggc ggacgtttct ctgtattaac
     8461 tgctgttggt ttgttaccaa tcgctgttag tggtgcagat atcgatgcat tgatgcaagg
     8521 tgccgctgat gcaagcaaag catactcaag cgacaaacta gaagaaaacg aagcttacca
     8581 atacgcagca atgcgcaaca ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa
     8641 ctatgaacca ggaatgcaat acttctcaga atggtggaaa caattgtatg gtgaatctga
     8701 aggaaaagac caaaaaggca tctatccttc aagcgcaaac ttctcaactg atttgcactc
     8761 tttaggacaa ttcatccaag aaggacgtcg caatattttt gaaacagttg tgaaggttga
     8821 aaaacctcgt aaatcaatca caattcctga acaagcagaa gatttagacg gtctaggcta
     8881 cttgcaagga aaagaagtgg acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc
     8941 tcatacagac ggtgacgtgc ctaacttgct agtcaaaatt ccagaaatgg atgcttatac
     9001 attgggttat acaatgtact tctttgaaat tgctgtaggt atttcaggtt acttgaatgg
     9061 aatcaaccca ttcgaccaac caggtgtaga agcttacaag aaaaacatgt tcgcacttct
     9121 aggcaaacca ggctttgaag atcttgctaa agaattgaac gaacgcctgt aagataaggg
     9181 tttttcgcta gatttaaata atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg
     9241 tacccattta ggtactcaaa catactgcat cgtttattca gaataagggg ctgtgacaag
     9301 acgtttgtca cagtcccttg tttgtataga aatcgattga ttgccttgct ctttagtttg
     9361 ttttaggact ttaagaacag tgaggctgac ttcgaaggtt aattctgttt ttttgttttc
     9421 tagaatcagt aagcaaaaaa gcactcactt cgagtgcttt tttgcgatat attcttagct
     9481 taagataggc ctaatttttc ttttacagat gagggtacct tatcgctggt aattactttt
     9541 ttctctatga tccgcttttc gcttgctttc aactgcaaga tcgttccttc ggggtaagca
     9601 gaaccattgg cagagtcacc agaagtaatg acatcgaaat ctaactgtct tttttcaccg
     9661 ttttcagcat aagcatctac gttgtattta aaacctcttc caattacttc cccattatct
     9721 gctttgatat cagtttctgc aggcattgga gctttgacta ccgcataata atctttacct
     9781 acataagtag actgatagta gtcccaacct ttccatccta taaaagcaat caatatgata
     9841 acgatcaacc ctaaaatttt tttcatgaaa gatctccttt tttattttta ttatacccgt
     9901 ggtgctagtt aattttaata tcctaaagaa tatctcaaag ttccttcaaa acgtcgttgt
     9961 gctcgactta atagaacact gtaagccagt aatatgcttt caaatctact ctctagccct
    10021 ttaacagaac gtgagttgaa attttgacaa cctaattttt ctaaggaaga aaaaacagtc
    10081 tcaactgt
//
LOCUS       DANKMN010000093         9941 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000093 DANKMN010000000
VERSION     DANKMN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..1189
                     /locus_tag="PW224_002553"
     CDS             <1..1189
                     /locus_tag="PW224_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2909184.1"
                     /translation="IYKGTLLYKPEECSHCLCVVPSRIIRWGTTTVRLLLNDASEYRT
                     YLELKKQRFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNI
                     STSSVYRVMKRFYRPLNPFKQALPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIV
                     ENRRLPYLECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHI
                     GRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDY
                     LTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTL
                     RTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTF
                     LTK"
     gene            1309..1509
                     /locus_tag="PW224_002554"
     CDS             1309..1509
                     /locus_tag="PW224_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2909185.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1585..1782
                     /locus_tag="PW224_002555"
     CDS             1585..1782
                     /locus_tag="PW224_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909186.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(2079..3626)
                     /locus_tag="PW224_002556"
     CDS             complement(2079..3626)
                     /locus_tag="PW224_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDL2909187.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3710..4513)
                     /locus_tag="PW224_002557"
     CDS             complement(3710..4513)
                     /locus_tag="PW224_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2909188.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4516..5298)
                     /gene="phnE"
                     /locus_tag="PW224_002558"
     CDS             complement(4516..5298)
                     /gene="phnE"
                     /locus_tag="PW224_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL2909189.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(5295..6074)
                     /gene="phnE"
                     /locus_tag="PW224_002559"
     CDS             complement(5295..6074)
                     /gene="phnE"
                     /locus_tag="PW224_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL2909190.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(6046..6837)
                     /gene="phnC"
                     /locus_tag="PW224_002560"
     CDS             complement(6046..6837)
                     /gene="phnC"
                     /locus_tag="PW224_002560"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDL2909191.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6898..7827)
                     /locus_tag="PW224_002561"
     CDS             complement(6898..7827)
                     /locus_tag="PW224_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2909192.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            8022..8705
                     /locus_tag="PW224_002562"
     CDS             8022..8705
                     /locus_tag="PW224_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDL2909193.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8866..9909
                     /locus_tag="PW224_002563"
     CDS             8866..9909
                     /locus_tag="PW224_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2909194.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         3032 a         2036 c         1978 g         2895 t
ORIGIN      
        1 gatttacaaa ggaactcttc tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt
       61 cccctctagg atcattcgtt gggggacgac aaccgttcgg ttgttactga atgatgcgtc
      121 tgagtatcgc acttatctgg agttgaagaa acaacgattt aaatgtaagt cttgccaaag
      181 aacattcgta gcggatacct ctgttgctga aaaacactgt ttcattagtc aaaaagttcg
      241 ttggtctgtc attgctcgct taaaagagaa tacttccatg acggaaatcg ctcgtcagaa
      301 aaatatttcc acttcttcgg tctatcgtgt catgaagcga ttctaccgcc cattgaatcc
      361 tttcaaacag gctttgccaa aggtcttatg ctttgatgaa ttcaaatcgg ttcgaagtgt
      421 tgccagtgca atgagtttta tcatgatgga cggacaaagc catgcattat tggatattgt
      481 cgaaaatcga cgcttgcctt atttagaatg ctatttttct cggttttcat tagcgactcg
      541 tgaagccgtt caatggattg ttattgatat gtatgcgcct tatgtttcat tggtgaaaaa
      601 gcttttcccg aatgctcagt tgatcatcga ccgtttccac atcgtccaac atattggcag
      661 aaccttcttg aatcatcgag tgaaagaaac cacggcacgg cttaaaggaa cttcccactc
      721 tcaacgaggg ttagggaaaa aattgaaacg ctattggaaa ctcctacaaa aagaagaagc
      781 aaagttaaac tacgaaaaaa gggtttggcg tgccagtttt aaagattatt tgactgaaag
      841 tgagattgtc gatcgactgt tatctgcttg tcccaacctg cgccaaggct atcagttata
      901 ccaagacatt ctttatgcgg taaagaaaag agatcagtca ttatttgagg actgtttaaa
      961 cagagaagtc agcggtcttc ctgaaaccta tacaaccact ttacgaacgt ttaaaaagtt
     1021 tctgccacag atccaaaatg cgcttcacta cagctattca aatggaccat tggaatgttt
     1081 aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcagct tttacaattt
     1141 caaactacgg atcatgattc gtcatggcaa aacattttta accaaataaa cagaacagaa
     1201 agagagtttc ttctttctgt tctgtccaaa ttattctaag atttctcttt accaacatca
     1261 tttgacaaag agcctgtatt tattactgtc gaggacaatg gcatcggcat gattgaagag
     1321 gaacaaaaaa acatttggga tcgctattat aaagccgatc cgtcaagtaa aaatacaaag
     1381 ataggcgagt caggtcttgg attatcaatc gtgaaggaac tagtgcgcct gcaccacggt
     1441 tcaattgaag tgaccagccg aaaaaatgaa ggaacgacct tcctgataca atttccaaaa
     1501 gaaaaataaa attaccagta aaaaaccgtg gagagatagc tgctattgat ttttgctatg
     1561 tcttttcttt gagaggtatc cggtatggtg gagtgtctga aaaataaaaa aatggaggcc
     1621 aattttgtga aagctgttat ttctggaagt attgttttcc aagaagaata tcgagatatg
     1681 gaaaaagatt tcagtaaaaa aggcattgaa ttaattgatt atcctcgctc atgtccgatc
     1741 ctagacaaag agtatccaga aatcttgacg actttttttt aaatatcgca gcagcggatt
     1801 tattttttgt atttaatcaa gatcggaaag gggtattagg ctatattggt gccgcaactt
     1861 tttcagaaat gaagtattgt ctgatgcaaa atcttattca tggaaaaagg atcaaaatag
     1921 tcttatttaa ggaaccatca gaggaaaatt ttttgttttg atgaggtaga tcgctggcta
     1981 aagaatggct ggatggaaat cagacagcct aacaatttgg cataacaaag gtgtgggcaa
     2041 aaaactgctc acaccttttt gaatttttta tttatatact acaaaatcac ttgaaaatta
     2101 tgattcactt ttgcttcgat gcgtgggtga atattcaaat agtcggcaat taattcggtc
     2161 atgtcgattt ggatttctcg gacaattttc tccgcaccaa acatcgcata atttcctccg
     2221 ccgattgccc gatattgatt tgtcaccact tccagcattt gttgcggaag aacggggtga
     2281 cctttaaaac gaagttcggt aatccgttgt ccaacaggct gtttcatatc aatagtgtac
     2341 tgaatccctt catacatgtc ataattgtag tactggggct taggctgaat aaaggctgga
     2401 ttgaatacga tttggttatt ttctacagct aaataatttg cggtctgctc caacgccgct
     2461 ctcaaatctg caccatttat ttgaatcact gccagagtat tcggataaat atagttggtg
     2521 atgacgtccc gcatcgtgat cgtctcacca aagccttttc cttcattatt gaataacgca
     2581 gttccagaaa tctctgagcc gctggcaaat aattgtacac gattaataaa ctctacatag
     2641 ggatgttcct caagacgagc ctgcatcgga tcattaatac gcatatttcc gttcacttta
     2701 cctaagggtt gatccaacca tttctctagt ttttcttcta ccggtacgat cgctgcaaga
     2761 atttctggat caggctctga tttttcaact gagtgtaaag caacctggtg atctgtaaca
     2821 accacttgcc cctctttttt ctcgacggta agacgaattt caccgatgaa ctcgccacga
     2881 tagcctggtt gaacaacggg aactccatga accgtttcag caattttccg gtgctgatga
     2941 ccggtgaaaa gggcgtcaat gccttcaacc tgctgcaata gctcgtagcc ttcattctct
     3001 ccagttagcg cttctgtgtc ctcaccggtt aacaagtctt tttcaaatcc gccgtggtaa
     3061 ctaacgatta ccaagtctgc ttgtttcctt aatagaggaa catattcttt tgctgtttcg
     3121 acgatactct taaacgtaat tcctgctaag gtttctggct tttcccagtg aggaatgtat
     3181 tgtgtcgtta atcccaagat agcaattttt acacccacct tttcgataat cttataggcg
     3241 tttccaaagt atggttggtc atttttgttc accatattgg ctcctaatat aggcgcttga
     3301 taattcttaa tcgcttcctc gagataatcc acaccatagt taaactcatg atttcccaac
     3361 acttgaacat catagctcat ttggttaatc aagtctgtaa gctctttggc tgaaccatat
     3421 tctttagcaa gataataact caaaggagac ccttggatga aatcaccatt ttcgatcgta
     3481 attaccggtc catctgcttt tgcccgtaat tccttcagtt tcgacgatac tttagccgtt
     3541 cctaaggcca gatcggttct gccggaatag tctgttggtg aaacatagcc atgcatatca
     3601 cttgtctcta aaaacgtaat gttcattctg cgtgctcctt tagctgactc aaaatagaaa
     3661 aaccgaagcg tattcagctg ttcggtctct ttttttgatt tataatgcct taatccttca
     3721 tttgaagtgt ttttaaaatg ttgcctggaa taaagttttc tgttgtctgg cgaacaatcc
     3781 ttccttgatc gagatgataa aggttatacc catgacgttc ttcaaagcgt acgaagccat
     3841 cgccttcatc ttcaccaaca aaggacatac tcgatacagt aaaatagggg atcccattaa
     3901 tttcatcagt aaatgtgtga tgggtatgac cgtttaggat acagcgaata tcgtaaggtg
     3961 cgattagctt caagaaacgt tcggtaccag gccattttgg tatactgctt tggtgagaca
     4021 gtagatgatg atgggtgacc aagaaaatcg gttggtcttt ttcttgctct aaggcttttt
     4081 tcagccattg aaattgctga tcgtccacta taccatctgc gtatccatag cttgaattat
     4141 caaaggaaat cactgtaaac tcaggatatt tgactatctg gttgtacggg gcagaagagg
     4201 cggcttcatc gcaccagcct acccaaaaat gttctttgat atcgtgattg cctagagtaa
     4261 caagaatcgt cgtttctcct agcgcatttt ccagccaagt cttcaagtat cgataatcgt
     4321 ctatttcgcc atcttcggtt aaatctccgc tgataatcac aaggtccaag ggtgtttgct
     4381 gcaggagatg atgaatgcat tgctccagag ggataagggg attctgcatc gtagccagca
     4441 ttcccttata gccttcttca caagcctcat actctctgcg aaagtgtata tctgatatat
     4501 gcaatatatt cattcttatc tcaatctctt tcttataatt ccggttattt gatctaaaat
     4561 aaacacggtt acgactacgc caatcaaaat aatactcact ttgctccaag accgagtttg
     4621 aatcgcgaat atcaatggag caccgattcc gccagcgcca actaagccta aggtagccgc
     4681 actacgaacc gctatttcaa aatgatttaa tgctaaagaa caataaatcg gcatcaaata
     4741 ggggatgcga acaaagaaaa tcgtttgcca aaaatttgct cctaccgcat gcatttcttc
     4801 aacaagtcgc tcatccatcg cttccatttc ttctgtaaaa agtttgccaa gcattccgat
     4861 ctgatggacg ccaatcgcca taacaccagc aaaggggcca ggtccgacga ctttcacaaa
     4921 aataattgca aaaactagtt caggaaaagc tcgtaaaaca ttacagataa gcttgccaat
     4981 ttttgttacc catggatgtg cttgccagag gttaaccgca ctgatcaagg taatgggaat
     5041 cgctaatacc gtagcgatca ttgtccctaa acaggcgatt ccaatggtta ataatagcaa
     5101 actaatcaaa tcctcgccgc tgccatcata tagaaactgc caatcaggct gacctaaccc
     5161 tctcaaaacg tccattccca tggaaggaga gaaggtgctt aatccagagt aatccagagt
     5221 agcggccgac aaaataacga gaatcactaa agcaactaat gataaaaggg ttcttctgtt
     5281 catttcacga agcatcataa cagggacctc ctgacacgtt cacttaattt atcaacgatc
     5341 gcgacagtta ctaggatcgt tagaataatg attgaaactc gttcataatt caaaagcgca
     5401 agtgaggaat ttagaataac gccaatacca ccagctccga catatccaag gacagtagaa
     5461 gcacgaacat tcacctcaaa cgcgtaaaaa gcatagctcg caaactgact caaaacttgt
     5521 ggagcgatcg accaaatagc gatttgcatt ttatttgctc caacagccgc agcggcttca
     5581 atcgggccaa agtcaatggt ttcaattgct tgaaacatta attgtgacac cataccaaaa
     5641 gtaaagacag cgatagtcaa cacgccagtc gcttcaccaa ttccaaacac agcaacaaaa
     5701 agcgcggcta aaagcagggt agggatggtc cgtactaaac ctaaaagaaa acgaaagatg
     5761 gtagaaagaa gacggttttc tgtcactaca gtggtcgcta aaaaactaac agggatcgct
     5821 aaaattaagc caatgaccgt tccaagcaaa gacatttgaa gtgttttcac cataggctcg
     5881 actaatttag gtaaatagct aaagtctgga tgaatgaagc gtgataaaaa gacagtcatt
     5941 tgattggagt tttgaaaaac ctgagaaaaa tcagctgacg tgatccaggc acttccttga
     6001 aaacaaacaa ttaagaacag aagaataagc aggtgtttat accatttaaa gtgaacggtt
     6061 tctttcaagc tcatcgattt cgcctgcctt ttcttcaaag attgcattgc cataaatttg
     6121 ctctaagcgt tcatcggtta agtcgtctgg acgaccatca aagacaacct gagcagcttt
     6181 taacgcgagc acacgcgtag aaaactcacg agccagaggg actgaatgca gattgacaat
     6241 gactgtttta tccatcgttt gattgatatt ttttaggtcc atcataacct tttgagtcgt
     6301 gattggatct aatgatgcaa cgggttcatc cgctaaaata atgggggctt gctgcacgag
     6361 tgctcgcgca atggatactc gctgttgttg cccaccactg agctcatcac tgcgtgtctg
     6421 caatttatca gacaaaccaa ctttttccag tgcttccttt gtctgctggt agtcttcctt
     6481 cgtaaaaaga cctaaaatac ttttccaagt ggagtaatag cccaaacgtc cagatagcac
     6541 atttttctga accgtactac gctttaccag attgaagctt tgcgagatta gaccaatctg
     6601 gcggcgaatc tgtcgcagct cttttttatt cgcttttgta atagaggttc cattaatgcg
     6661 aatatctcct ttggtggttt ctgccaagcg attgatactg cgcagcagag tgctctttcc
     6721 agcaccactc aaccctatga tggaaacaaa ttctccatct tcaattgtta agtcaatatc
     6781 cttcaagcca acaacgccat tgggatagac cttcgaaaca ttgtcaaaaa taatcattat
     6841 ccttctccat ttttttaagt gtaagcaaaa aaaagcttgc tgacacttac tgatgtttta
     6901 ttctgctgct tttttcgtgt agtctctgat cgtgtcatag gcagaatcag cagcttcttc
     6961 atagccctga tgcccccaca aagccattgc ttctttgcca tctttatcat ccttcatatt
     7021 taagaaggtc tctttaattt tcgcttttaa atcttttgac atgtcaggct gtacagcgat
     7081 tgcgtcattc ggaatatcgc cttttgtcag gtacaggact tttaaatcct taaataaatc
     7141 attatctggg aaattcgaag caaaaacatt tcgcgcgcct tcaaaaacaa acgccgcatc
     7201 catttgcttg ttcaacactg cggtaatctc actcggaata tcattcacag tggtcatctt
     7261 tgtatctgca gtaggatcaa tcccagcatc tttcaattca gcgacaggat agatgtagcc
     7321 gctagcagag ttaggactta aggttgcgat attttttcct tttaaatcag ttaacttatt
     7381 caatccgcta tcggcacgaa ccaatacttc tcctttaaag gtgttcgtaa gtttgtcctt
     7441 taatggcaag ccagtttctt gatcataatc gccgagctga gaagtcagga ttgcatctgc
     7501 agctccagca tcctttgctt gaacataggc tgcaggcggc ataattccga catcgacttg
     7561 accagaagac atcgcttcga cgatcgttga ataatcagta gctaacgtca cttctacctc
     7621 acgatctaac tttttcgata aatacttagc aaatggtttt gcttttgcct ccatactacc
     7681 atcattgttt gttggaacga attgaacaac taatttttct ttgctctctt tttctgagct
     7741 gctacttgta ttcccagaac cgcaggccgc caaagtggtc aataacatga ctccgatgaa
     7801 cccttttgct aactgtttga actgcatttt tctcctcctg ttggatataa tttctaatac
     7861 gtctaaatca tagcagggaa tcaaaatcaa tgaagtgacg cacgtcctat tttacacaac
     7921 ttttacatac aatttacaaa caattataaa taattattcg tattgagccg aaaaacaatc
     7981 taagctataa taaagaaaaa cttcttagag gtgacgattt catgaaaaaa atccatgata
     8041 cgaagcaatt aactgcttac gttgagaagc acaacctgca ggattatatg gattcagata
     8101 tattggcaat ggcttcttta cttttttttg aaaaggaaga gcacctcatt caaacgggaa
     8161 aaacctcaga ttatttgtat tttttagtgg atgggacggt aatggtgtac tcgtattctt
     8221 ctgatacaca aaatatttgc atcgattacg caaagcccgc tacaccactc ggcgaagcct
     8281 cttcattgtg ggaattatcg ccgaaaagca gcgttaaagc agtgactaag tgcatctgta
     8341 tcagcattcc gctaaatcaa tatcgaacac agcttcaaaa agatgttcgc tttttgcaga
     8401 atatttgcca gttactaagt tatcgtttaa attccgggat aaatttggcg aactcactga
     8461 cagaacctgt tgaaactcgt ttagcaaagt ttattttgac ccatcacgaa aacgaacgtt
     8521 tctcatttcg gttaaccacc tgcgcctcaa tcttaaatgt cagttatcga catcttttac
     8581 gaacaatcac gaattttcgt gaggagaata ttatcgaaaa gaaaaagaat tattacttga
     8641 ttcatgatat cgaagcttta gaggacttag ctgagaatat aagtagtcca gtaaaagcag
     8701 aataacagct ataaaaaaac agcagtcaga agtgattgct ggttttttga tagtggagaa
     8761 attgggaaac ggaaaatctt cgttgaagag tagtgttcta tatattggta tgctatactt
     8821 ttaaaagttc aatgtgtcaa gacacataaa aaggggagaa aaattttgaa gaaaatactt
     8881 gtttcattgt tgtttttggt gtcattagta acgttagtcg gctgtagtca gtctcagggg
     8941 gagaagcaaa gtgaaaaaga gaaaagtaaa atcgctttgg ttcttggatt tggcggggtt
     9001 gatgaccgct ccttcaatca agctggctgg gaaggattag aggcttgggg aaaagagaaa
     9061 aagctgacag caaaaaaaga ttttacgtat atagaaacga acaatccaga ggataacagt
     9121 accaatatcg atcaggcaat cagaaacggc tttaccacca ttattagctt gagctacttg
     9181 caagcagagg aactggaaaa tgctgctaaa gataatccaa agttgaaatt tgccttggta
     9241 gatggtgaga ttactgggaa aaagaatatc tcatcagcaa cctttaaaga tcaagaaagt
     9301 gcgtatttag caggactagc tgccgcatat acgacaaaaa gtgatcaagt aggctttatt
     9361 ggtggaatgg aagggcccgt gatccaacgt ttccaaagcg gatttgaagc tggcgttaag
     9421 gcaggggcca cagcactgaa caaggaaatt actgttaacg tacaatatgc tgcatcctat
     9481 gattcgctag ataaaggaaa agctattgct gcttctatgt acaaagaggg agtcgatatt
     9541 ctttatcagg cggcaggaag tactggagct ggggtgttcc aagaagcaaa agcccgcaat
     9601 gaaaacgact atagcagaaa agtatgggtg attggtgtag atcgcgatca agcggatgaa
     9661 ggggattata aaatggagaa cggcgagaca gggaatttca cgttgacttc tacaaagaaa
     9721 aatgtcggtg cagaagtcaa agaatttgca gaaacttctc ataacgataa atttcctggc
     9781 ggcaagcatt tgacctttgg tttgaaagaa gaaggggtag atctgacaaa aggacaatta
     9841 tcagaggaag cctatcaggc agtttcagac gcaaaagaga aaatcatcag tggtgatttc
     9901 agactgtaga caaagtccat tttatggatg atgtctacag t
//
LOCUS       DANKMN010000094         9873 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000094 DANKMN010000000
VERSION     DANKMN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(22..267)
                     /locus_tag="PW224_002564"
     CDS             complement(22..267)
                     /locus_tag="PW224_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL2909195.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="PW224_002565"
     CDS             complement(381..959)
                     /locus_tag="PW224_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2909196.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="PW224_002566"
     CDS             complement(1036..1566)
                     /locus_tag="PW224_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL2909197.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="PW224_002567"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="PW224_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL2909198.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="PW224_002568"
     CDS             complement(2423..2818)
                     /locus_tag="PW224_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL2909199.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="PW224_002569"
     CDS             complement(2815..4227)
                     /locus_tag="PW224_002569"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL2909200.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="PW224_002570"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="PW224_002570"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL2909201.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4846..5085)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5082..6542)
                     /locus_tag="PW224_002571"
     CDS             complement(5082..6542)
                     /locus_tag="PW224_002571"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL2909202.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="PW224_002572"
     CDS             complement(6712..7869)
                     /locus_tag="PW224_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL2909203.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="PW224_002573"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="PW224_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL2909204.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="PW224_002574"
     CDS             complement(9336..9863)
                     /locus_tag="PW224_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL2909205.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DANKMN010000095         9667 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000095 DANKMN010000000
VERSION     DANKMN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..650)
                     /locus_tag="PW224_002575"
     CDS             complement(<1..650)
                     /locus_tag="PW224_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2909206.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="PW224_002576"
     CDS             975..1391
                     /locus_tag="PW224_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2909207.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="PW224_002577"
     CDS             1388..1858
                     /locus_tag="PW224_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2909208.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="PW224_002578"
     CDS             1872..2645
                     /locus_tag="PW224_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2909209.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="PW224_002579"
     CDS             2659..3471
                     /locus_tag="PW224_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2909210.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="PW224_002580"
     CDS             3525..4490
                     /locus_tag="PW224_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2909211.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="PW224_002581"
     CDS             4606..7329
                     /locus_tag="PW224_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2909212.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="PW224_002582"
     CDS             7342..7974
                     /locus_tag="PW224_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2909213.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9667
                     /locus_tag="PW224_002583"
     CDS             8873..>9667
                     /locus_tag="PW224_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2909214.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLYL"
BASE COUNT         3261 a         1477 c         1884 g         3045 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtattta
//
LOCUS       DANKMN010000096         8493 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000096 DANKMN010000000
VERSION     DANKMN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..173)
                     /locus_tag="PW224_002584"
     CDS             complement(<1..173)
                     /locus_tag="PW224_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2909215.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="PW224_002585"
     CDS             complement(593..1714)
                     /locus_tag="PW224_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDL2909216.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="PW224_002586"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="PW224_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL2909217.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="PW224_002587"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="PW224_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL2909218.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="PW224_002588"
     CDS             complement(4304..5215)
                     /locus_tag="PW224_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2909219.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="PW224_002589"
     CDS             complement(5232..6239)
                     /locus_tag="PW224_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2909220.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="PW224_002590"
     CDS             complement(6236..8365)
                     /locus_tag="PW224_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2909221.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8493)
                     /locus_tag="PW224_002591"
     CDS             complement(8370..>8493)
                     /locus_tag="PW224_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2909222.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DANKMN010000097         8421 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000097 DANKMN010000000
VERSION     DANKMN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            471..2108
                     /locus_tag="PW224_002592"
     CDS             471..2108
                     /locus_tag="PW224_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909223.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="PW224_002593"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="PW224_002593"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2909224.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="PW224_002594"
     CDS             complement(3184..3819)
                     /locus_tag="PW224_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2909225.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="PW224_002595"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="PW224_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL2909226.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6401
                     /locus_tag="PW224_002596"
                     /pseudo
     CDS             5405..6401
                     /locus_tag="PW224_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            6417..>8421
                     /locus_tag="PW224_002597"
     CDS             6417..>8421
                     /locus_tag="PW224_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2909227.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQ"
BASE COUNT         2782 a         1547 c         1293 g         2799 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa a
//
LOCUS       DANKMN010000098         8297 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000098 DANKMN010000000
VERSION     DANKMN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(52..1518)
                     /locus_tag="PW224_002598"
     CDS             complement(52..1518)
                     /locus_tag="PW224_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL2909228.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1905..2501
                     /locus_tag="PW224_002599"
     CDS             1905..2501
                     /locus_tag="PW224_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2909229.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2559..3041)
                     /locus_tag="PW224_002600"
     CDS             complement(2559..3041)
                     /locus_tag="PW224_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909230.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3147..3344)
                     /locus_tag="PW224_002601"
     CDS             complement(3147..3344)
                     /locus_tag="PW224_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909231.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3639..4397
                     /locus_tag="PW224_002602"
     CDS             3639..4397
                     /locus_tag="PW224_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2909232.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4457..4885
                     /locus_tag="PW224_002603"
     CDS             4457..4885
                     /locus_tag="PW224_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909233.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4931..5003
                     /locus_tag="PW224_002604"
     tRNA            4931..5003
                     /locus_tag="PW224_002604"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4964..4966,aa:Gln,seq:ctg)
     gene            complement(5153..5524)
                     /locus_tag="PW224_002605"
     CDS             complement(5153..5524)
                     /locus_tag="PW224_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2909234.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5739..5924
                     /locus_tag="PW224_002606"
     CDS             5739..5924
                     /locus_tag="PW224_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909235.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6007..6195)
                     /locus_tag="PW224_002607"
     CDS             complement(6007..6195)
                     /locus_tag="PW224_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909236.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6219..6482)
                     /locus_tag="PW224_002608"
     CDS             complement(6219..6482)
                     /locus_tag="PW224_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909237.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6640..6897
                     /locus_tag="PW224_002609"
     CDS             6640..6897
                     /locus_tag="PW224_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL2909238.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6968..7159)
                     /locus_tag="PW224_002610"
     CDS             complement(6968..7159)
                     /locus_tag="PW224_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909239.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7300..7620)
                     /locus_tag="PW224_002611"
     CDS             complement(7300..7620)
                     /locus_tag="PW224_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909240.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7643..8077)
                     /locus_tag="PW224_002612"
     CDS             complement(7643..8077)
                     /locus_tag="PW224_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2909241.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2635 a         1636 c         1313 g         2713 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tatttaagaa atataaacta attttctata
     1621 aaagcaagta atttttaatg gcaatatcat aaaaaaaaga agatagagat ttaataatta
     1681 tatatcagct gtttttttca aataagcaaa aattcctaaa acacaacaat gaaagcgata
     1741 actaagaaaa tccttacata acgatttttc ttattaaaat tgttttttga acttcgctta
     1801 ttttttgtga gcatatctgt ttacttactt tttgtaaggt gctatacttc atttcgtaat
     1861 caattaaaaa aatgcaaaac gcaaatcaaa ggagaaattt tattatgaca acttttactg
     1921 acactttgaa aaaccgccgt tcaatctatc atctaggacg taatgtttca ctttctaatg
     1981 aagaattaac aactttgatc aaagaagcaa ttaaagaaag cccaactgct ttcaatgctc
     2041 aatcaacacg cgcagttatc cttttcggag atgcacatga aaaactttgg gaaatcactg
     2101 aagaagcact tcgcccattg actccagcag aagctttccc aaatacacaa aacaaattag
     2161 ctggtttcaa aaatggttat ggtacagtat tattcttcaa agatactgac gtggtaaaag
     2221 gtctacaaga acaatttgaa ttatatgcag acaacttccc agattggtca gaacaatcaa
     2281 acggaatcgc aacagctaat acttgggtag cattagttga taaaggattg ggcgcaaact
     2341 tgcaacatta caacccagtt attgacgaag cagttgcaaa agaatggaat atcccttcaa
     2401 actggaaact acgttcgcaa ttagtattcg gttcaccaga aacaccggct gaggaaaaag
     2461 aatacatgaa cgatgccgat cgcttccgcg tatttggata attttatttg aaataaaaaa
     2521 cagaggttgt gacaaacatc ttgtcgcaac ctctgttttt attctggatc aatcataata
     2581 tcaatctctg cctcatcgtt taagcgttgg gacaaaaatg gaactaattc ttctaatgat
     2641 gcagaaaatt tcagcatcgg tcctcgccat ccgtttcgga aaaaattcat ttttacgatt
     2701 ttagaacctt ttggctgata taatacaatt ttattgatct cagaaaaagg aacacctttt
     2761 ttgtcaaagc tattcaaaac aagtccttct tctgtgattc ctctactgtc caacaaaaaa
     2821 ctaataaaaa tcattgctga caagatccct tttgccaatt catctgctgt aggatgactg
     2881 atgagtgtat accaaatgac tagtgacact aagaagggta tcaccaagta gacccatcga
     2941 tttcgcacag tccgataaat aatttctttt cgaaccagcc aaagaaataa aaacatcaca
     3001 acaacgaata aaaacaatag aaccgtaaag aatgttacca tgacaactcg cctcgctttt
     3061 tcttctccag aaaataggaa aagaatactg atttaccttc tcaaagaaat tggacttgca
     3121 gcacgccgca cgtccaattg ttttctttat tcaaatgtat catattttgc aacaaccatg
     3181 cttgcttttt catctaataa ttcttggtcc tctgcagcac attgggcaac tttgaccacg
     3241 gcgcagtttg tcatttttga tacaacttca ccaatgaccg tttttgtaaa accaattggt
     3301 ttgcatgcat attgttctcc taaattaatc atttcttccg tcatcaaaat cctcctaaac
     3361 tgctggaact tttctccaca ttaccactgc tatttctttg gagatttaac tcattgattt
     3421 ttatttacag ttaaaaatca accttacagg cattatagcg tactgttttc gttttcacaa
     3481 ttaaaacagc tgttgtattt agaagaattt ttttcaaaaa attaaaacat attccttaaa
     3541 aactaattat ttcctacata ttaaaatata atacttccat ttccaataaa aatacattta
     3601 taataggaaa gataaacagc tgaaaaggga gatttcaaat gaagaaaaaa acgttcaaaa
     3661 aactattgtt tctagggggc acagctgctc ttttattaag cggtacaagt tattacctac
     3721 atatccattc tccagaacat acaatgaaca ctgcacaaaa aagttcaaca caaaaaacga
     3781 aaaagacaaa agagaaagca gaagttttac tagatgttcc attagagagc cagtttgatt
     3841 ctccttcttt agaaaatggc tgtgaagtca cttcgctttc tatgctttta tctttttatg
     3901 gttatgaaac aactaaaaac cagttagctg agcaattgga ttacgtccct gttttcaatg
     3961 cagatgggac tcatggcgat cctaatgaag gatttgttgg tgatatcagt ggcggagact
     4021 gggctatggt cgtctatgtt cccccagttg cctcccttgc tcagcaaatc gtccaaacgg
     4081 attatcacac ttttccaaaa accgatgctc aactcgatga tatcaaaaaa gcactatcta
     4141 aaggaaatcc tgtctggact tctgtgacta tcaattttga agtcccagat gaaactgatt
     4201 ttatgacttg gaccacaaat aatggagaag taagagtcac tcctcttgtt catgcttgtg
     4261 tagtcactgg atatgacaaa gaaaatattt atgtcaacga tccctatggt gtaaaaaatc
     4321 gagcagtacc gatcaacgat ttttcggcga ttttcactgc aatgggtgga cagatgctaa
     4381 ctttggagaa aagctagtaa ttcaaacaaa aatcccttat actcaagata ccaaaccttt
     4441 gaagggagcg tagataatgg aaaaattcac acataaaaaa atggatccaa atgaaattcc
     4501 gattattttt gtccgtgact gcaagggaaa tgttcaaggt aaagtctcta ttaatgagtg
     4561 gaacgagcgt cgcagacctg ctaccctcaa tgaactggaa atcaaattgt atcgtcaatc
     4621 tcttgtttat tacgcagatc aagagtacga gaaagccacc gatttattga aatttctaat
     4681 tgctcgaaca gagtatactc gctttgaata tattgaacgt ttagcaaaca tctatcatat
     4741 tatgaatgaa cctgtcaaag aatatcagtt attggacacc gtcttgtcag tagcagaact
     4801 gattgccctt cctgctggat tagaaaagaa actagtacgc cgccttttac gagtaaagca
     4861 acaactatcc gatcaagaaa aataacttgc gtttttttag gaaagtgcta tgattatttt
     4921 gttgaataaa tgggatgtag ccaaactggt aaggcacttg actctgaatc aagcaattgt
     4981 aggttcgaac cctgccatcc caatttgatg tgcttgctga aaaaatgaac cccctgtaaa
     5041 agaacaatct ttttgcaggg ggattattgt attattttta ctacttgtat ttagtttaca
     5101 taattaaatt atgaataata aaatcgggcg tattcttaat acaaaaactg atttacccaa
     5161 taattgctaa tgctttctcc ggaacttttt ctctaggaac ttcctcatac gtttctatgt
     5221 gtgctccttt attgctaatt ttcaaataat gattttcttt taacacagaa agaccattaa
     5281 attcaaccgt tcgttctttc ccattggcat cagcagcttt ctgcacatac cttgctgtac
     5341 catattcatt tacttcctct ggtttttgtg tctttacata gacctctcct ttttcaacta
     5401 atggatttag ctgatcaagt ataccaggga tttctccata acttccttca gtataatatt
     5461 tcagtccaaa tagcaaaata ccactaatcg ccgccagtcc aatcaaatat ttaatcactt
     5521 tcatctttct acctccttgt tatatattca gtatatgggt cagttaaaga aattccatcc
     5581 gtcccaagtt ggagattttt ctcctacttt agaagcgaga atcgagtgtt tttttagtaa
     5641 aaatgatcgt tatttgatat tattgacgtg tggataaatc agtttttctc gttttgtcca
     5701 tgtaaaaaat catgttgata gcaaaggatg tgttcaaaat gaatcaagaa gaattatttc
     5761 aaaaagtcaa ggaaatgatt aagaacggca atttcgatgg cgcaaaacga tttatcgaag
     5821 aacataaaga acaattagga ccttataaag agaaagccca aaatctttta aaagatgtaa
     5881 atatcgacag tgtcaaaaac aaatttaaaa atctattcaa ataagtaaaa ctttcacttt
     5941 tgtaaaatca aaggaggaca atgctgctgc atcgtcctcc tttggttctt ttctgaatac
     6001 ttcatactaa aataaacgtg tcggatcgta atgataagtc acattgccgt tcagtgaggc
     6061 ttttcccacc acatatttac gattctccaa cttcagccac gcttctcgaa aagccattaa
     6121 ttcttctctt tttacatcaa tcttttcgat ttttccatcg atcaaatctt gaataagttg
     6181 ttcatatgga ttcatttttt tcctccatct gctgtcaatc aataagttgt aaaaataggt
     6241 tcatatgctt ttttggtatc tagatcttct ctgactttga ctgtcgtata aataattttt
     6301 tctactggga tactataata acttccttca ccattattga atcggtgaac gtctttcttt
     6361 gctaaaatac catttatttt attcgtcagt acacgttgtt cgacactgtt acttacaaca
     6421 tagtaaatca cccgaatatt attgatcaaa tgcaaatcga tttctaacac tacatgatcc
     6481 atgaaaatcc cactccttct ttcactttga ttatagcaag tttctcctct aatttcaaaa
     6541 tcgatacaga ttttaccgga taaatccaca aaactgcttg cgccatttcg cacaaagtgt
     6601 tattttgtat catggacttt atttatcgga ggcaatagta tgatcgcgat cggtcaattt
     6661 gttttttata ttcctttttt tattatgata agcattcttt tctattacat aaaatggaca
     6721 aaaaagaagt tttcagtttt actggcctct ttacctgctg tatattttac ttatcaaatt
     6781 ttttctttta ggcattggga aacaacgtct gtattaataa cacacatcat cgaattgacg
     6841 ctttcagtaa tttttcttat tatttggatc tattttttat ataagaatca gaattgaatc
     6901 cctcgcatgt ttaaaaagaa aacgattaaa gatgcagcaa atttctgccg atctttaatc
     6961 gttttttcta ttctgttata ccaaaaaaat gtttgaatcg gtgaaggatc gttccttgtt
     7021 ttaattctat aaattcttgt gcgatcgggt acttttcttc caaattttct ttgttctttt
     7081 cgatcaggct tttcgcttct tcatattttt tatctgcaca tagtgctttt acttcctgta
     7141 atacttttcc tgcttccatt tgcctcaccc tcttgccatt ttattgcttt aagttttatt
     7201 taagttatac gcattattct agtttcatcc caaataacct tttttcattt ttactcctcg
     7261 aactaatcag ttttctaaag gctgattggt tcgtcttttt tatttttcct ccatttcatt
     7321 ctgctcttct gttaatttat ctaatgcttt gattgatgct gcggctcttg cataatcata
     7381 gctgtataat tccataaatt tatcaggcag aacaaaccac gctggatgat aaccgttagc
     7441 tgtgacaggg aatcctttat cttgttggtt atctccatcc catctcagag caacaccttt
     7501 ttttcctttc cactttacta aagcgatcga gtatcccttc tttgtttgtg tgccgtcttc
     7561 aataattgct aaaatttcta ttttttcttt tggcgcacgg acttcttgag gacgaaccat
     7621 tttctattcc tcccttatct cattacttca cgaccaacac actgcaaggg gcatgtgata
     7681 cgacataaga agcggtcgat ccaactaatg cttgttgtat agcacctttt cctgtcgctc
     7741 ccataacgat caaatcaata ttttcttgtc cagggatgac attggcaatc aaatttcttg
     7801 gatctccagt ttcaactatt gcgtggatct cagcaatgcc gtactctttg gcatcattga
     7861 tttttttcag catggctact tctgttcttt gtttttcatc tgcatagatt tttgaaaagg
     7921 cataggcact atgggtcagc tcaactttgt tgatgattga cacgataaat aagtgggctt
     7981 cattgtcctt tgctaagtca agggcttcag cgaacgcttt gatcgattgt cttgatccgt
     8041 caactgctac catgatgttc tggtatcttt tactcattgg aaccgcctcc tttttctttt
     8101 attgtaacat ctcaagcaaa aatacaaaaa atatacgcaa tatgtaaata aaatgatgaa
     8161 caaaagcaat ttctttcaag ttatttatac atttcttttc tcatcagaac acctgttcta
     8221 atcccattat ttctgcatta tgtgaaattc ctcatcattt ttcacatcga gcgaacatca
     8281 ttctgttaca aaaaacg
//
LOCUS       DANKMN010000099         7789 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000099 DANKMN010000000
VERSION     DANKMN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            158..730
                     /locus_tag="PW224_002613"
     CDS             158..730
                     /locus_tag="PW224_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2909242.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            875..1579
                     /locus_tag="PW224_002614"
     CDS             875..1579
                     /locus_tag="PW224_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2909243.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1807..2145
                     /locus_tag="PW224_002615"
     CDS             1807..2145
                     /locus_tag="PW224_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909244.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2130..2984
                     /locus_tag="PW224_002616"
     CDS             2130..2984
                     /locus_tag="PW224_002616"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2909245.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3131..3364
                     /locus_tag="PW224_002617"
     CDS             3131..3364
                     /locus_tag="PW224_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL2909246.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3562..5466
                     /locus_tag="PW224_002618"
     CDS             3562..5466
                     /locus_tag="PW224_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2909247.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5776..6138
                     /locus_tag="PW224_002619"
     CDS             5776..6138
                     /locus_tag="PW224_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2909248.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6155..6766
                     /locus_tag="PW224_002620"
     CDS             6155..6766
                     /locus_tag="PW224_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HDL2909249.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6868..7776)
                     /locus_tag="PW224_002621"
     CDS             complement(6868..7776)
                     /locus_tag="PW224_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2909250.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
BASE COUNT         2489 a         1305 c         1688 g         2307 t
ORIGIN      
        1 aatttaatag agaagcctgg cgtctattaa gaaataattt tcttgatgag cgtcaatttt
       61 tttaacggaa atctatccta tactaaattc agtaaagaaa aaggaggaat caaagaaagc
      121 tattttaaac aaaccaaaat tataggagga ataaatcatg actgaaaata aagctactca
      181 agaaagaaca ccccaagaaa gattgcaaga agaacaagaa cacaaggaac atgttcatca
      241 tacgaaaatt aatgcagcgg ccattgcgga tcatctttta ggcaacatgc atacattaca
      301 tgtgaaactg caccaatatc actggtatgt aaaaggagcc aatttcttta cgttgcatga
      361 aaaatttgaa gagttgtata acgaaaatga aaaatggttc gacaaacttg cggagcgtct
      421 aattacttct gggcataaac ctgcttcgac aacggttgaa tttgaaaaat attcgatgct
      481 ttcggaagat gcagttaata aatacgctaa agcagaagac atggttgaaa atattatcga
      541 ggacttcaga agcactcgtg acctaactat tcgcgcgatt cgtttagctc aagacgaggg
      601 tgacgatgct ttagaagata cattgattgc ttttaaaaat gacttagaca agaacatttg
      661 gatgctgcaa gcgttcattg gtaaagaagc attagaagat gatgacgctc ttgatgagga
      721 tgaagattaa ggtaaaagta gaagctgatt tagggattgg aattctttcc agtccttttt
      781 ccattttctt gatgtgcatc aatttttttg tagtaagaga cttatataat aaaagcagcg
      841 aatataaaca aaataaataa agggggagaa tgaattgact aaacataccc atcatgaaat
      901 tggcgaccac gcagcctgta tccgcttggt tccaatattc aatcacttag aggatagcgc
      961 gatgaattac attgctgaaa aagcccacac gaaacagtat caaaaaggag agtttctgtt
     1021 tcgttcacaa gaaaaagagg atactttata tattatcaat cgtgggaaaa tccgtattta
     1081 tcgattggca gactctggca aagagcagct agtgcggatc ttgaaccctg gtgatttcac
     1141 cggagaatgg accttgttca acccggatgc agtacacgag gaatatgccg aagcaacccg
     1201 agataccgtt gtctgtatga ttcagcaaaa agatgtccaa gattttctgg aacaataccc
     1261 agctatttct ttaaaactat tggcggaaat gtctaaacgg ttagaaagtt cagaacgtca
     1321 aacgacacaa gtagccgtgg agagtgtcat tacccgttta gttttgtttt tggcagaaaa
     1381 tgtggaacct gaaatgggca actctcccac catcaccctg ccgatggcaa aaaaggacat
     1441 tgcttcctat ttaggaacga cacctgagac catcagtcgc aaatttggag aattggagga
     1501 cgaaggattg attcaacagc tgtctgggaa aaggatcaag attcaggatt tagatgattt
     1561 attgctttac agcgaataat cggacacact tttttctaca cgttggtgtt ctgtcgctct
     1621 tcattaacaa cgggacagaa ctcagtgctt ttgcttccat ctgtccgttt ttggcttttt
     1681 taagacagaa cgaccttcat tcattggaaa aataaaagca caaacatgat aaccctcaag
     1741 tttttttgtt ttatttttct ttaagatagg gttgtcgaac aaaacgtaaa aataagtagg
     1801 gtgaatgtgc attgtttttg ggtgatagtt gccatcacgt tgtcagcagc gggggcattc
     1861 tttgtcgtcc ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt
     1921 gaggcagata ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc
     1981 ggacacggaa aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact
     2041 gtttggtttc catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg
     2101 cgaaaaattg aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc
     2161 atgtggtgga gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca
     2221 tattagcggc tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata
     2281 ccattatcga tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt
     2341 atgtgttatt tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga
     2401 atggcggcat tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg
     2461 caaagaaaaa aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt
     2521 tagcccagtc cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg
     2581 ttactcgtca attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata
     2641 tgaatggtac ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct
     2701 ttgtcctcat cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt
     2761 tgagttacgt tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca
     2821 gtttatggat tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg
     2881 gcgcgattgt agtatggttt atacgcagac aagattatcc accaatttct tattattctg
     2941 atggcaataa ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag
     3001 aagtgcaaaa cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg
     3061 taaagaggaa aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga
     3121 gataggaatt atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat
     3181 gcaaaaaatt gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt
     3241 attattcaac tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga
     3301 gattcaaaaa gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt
     3361 ttaacagatt aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt
     3421 ttttgttgca atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga
     3481 tgtcagtcaa ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa
     3541 ttaaatttgg agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc
     3601 gtcatcagtg gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta
     3661 ggactttcag agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt
     3721 caagcgtttc aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca
     3781 gcgattggtg cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc
     3841 ttattcggac actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa
     3901 ttgactgaaa tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa
     3961 gtagaagtgg atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt
     4021 ccagtagatg gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt
     4081 gaagctattc cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa
     4141 aacggaacga ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt
     4201 attgaactcg tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc
     4261 ttttctaaat actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt
     4321 caaaatattg agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc
     4381 ggagtgcctg tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta
     4441 aaagggagtg aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca
     4501 ggtactttaa ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct
     4561 gctgaaacgc ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa
     4621 gcggtcttaa atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg
     4681 aaaggcggcg gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc
     4741 ttgatggaaa aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt
     4801 gaacaacaag ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg
     4861 ggcattcgcg atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg
     4921 ggcgtgaaaa atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc
     4981 ggcgaacttg gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat
     5041 atcggaaaac ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat
     5101 agtccgtcct tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg
     5161 attgaaacat ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta
     5221 ggattagtaa aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta
     5281 gtgttggtct tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg
     5341 ttggttcatg aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat
     5401 aagttgaaag gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata
     5461 tcttaagaat aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg
     5521 aaactcagat ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga
     5581 gtatagagta tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat
     5641 aaaatttaaa ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat
     5701 taaggtagat tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa
     5761 tgtgaggtga gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta
     5821 aatgtaataa aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     5881 tgtttcagcg attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg
     5941 tgtgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     6001 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat
     6061 tctttagcga atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat
     6121 ctatctatga aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat
     6181 cgctgtttat atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca
     6241 aagccacaca aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat
     6301 tttggtagcc gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat
     6361 cattggtctt ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga
     6421 agaggaggaa gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt
     6481 attctggaca gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat
     6541 tccatacttt acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat
     6601 ctctgttgcg gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga
     6661 aacgttagaa aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt
     6721 tattatgata gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg
     6781 aaagataaaa agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa
     6841 tgaagtattt tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc
     6901 ttctttgctt gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac
     6961 ttctgcaatt tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac
     7021 cgtgatgata agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc
     7081 ttttacagca ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg
     7141 cgaaccattc aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat
     7201 ataaagtgca atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat
     7261 tagaatactc acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt
     7321 caacatgctc tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa
     7381 tgcaatagct acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat
     7441 caaacgtggc acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt
     7501 tatcagcgcg cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt
     7561 tttctctgaa taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc
     7621 atgaactgcg tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct
     7681 tatagagaaa ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg
     7741 attcgagtga ttatggtctt tatctgattt tgacatttaa aaacctcct
//
LOCUS       DANKMN010000100         7599 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000100 DANKMN010000000
VERSION     DANKMN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(942..1355)
                     /locus_tag="PW224_002622"
     CDS             complement(942..1355)
                     /locus_tag="PW224_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL2909251.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="PW224_002623"
     CDS             complement(1811..2296)
                     /locus_tag="PW224_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909252.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="PW224_002624"
     CDS             complement(2435..2650)
                     /locus_tag="PW224_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL2909253.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="PW224_002625"
     CDS             2874..3674
                     /locus_tag="PW224_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2909254.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="PW224_002626"
     CDS             3693..5561
                     /locus_tag="PW224_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2909255.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="PW224_002627"
     CDS             5554..6045
                     /locus_tag="PW224_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL2909256.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="PW224_002628"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="PW224_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL2909257.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..>7599
                     /locus_tag="PW224_002629"
     CDS             6605..>7599
                     /locus_tag="PW224_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL2909258.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DANKMN010000101         7257 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000101 DANKMN010000000
VERSION     DANKMN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..412
                     /locus_tag="PW224_002630"
     CDS             <1..412
                     /locus_tag="PW224_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2909259.1"
                     /translation="ESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDY
                     RQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKT
                     DHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            488..706
                     /locus_tag="PW224_002631"
     CDS             488..706
                     /locus_tag="PW224_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909260.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            693..3371
                     /locus_tag="PW224_002632"
     CDS             693..3371
                     /locus_tag="PW224_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909261.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3362..3559
                     /locus_tag="PW224_002633"
     CDS             3362..3559
                     /locus_tag="PW224_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909262.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3563..4174
                     /gene="mobC"
                     /locus_tag="PW224_002634"
     CDS             3563..4174
                     /gene="mobC"
                     /locus_tag="PW224_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL2909263.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4159..5550
                     /locus_tag="PW224_002635"
     CDS             4159..5550
                     /locus_tag="PW224_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2909264.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5766..6080
                     /locus_tag="PW224_002636"
     CDS             5766..6080
                     /locus_tag="PW224_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909265.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6073..6567
                     /locus_tag="PW224_002637"
     CDS             6073..6567
                     /locus_tag="PW224_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909266.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            6635..6949
                     /locus_tag="PW224_002638"
     CDS             6635..6949
                     /locus_tag="PW224_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909267.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7048..>7257
                     /locus_tag="PW224_002639"
     CDS             7048..>7257
                     /locus_tag="PW224_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2909268.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         2774 a          984 c         1410 g         2089 t
ORIGIN      
        1 tgaatcactt tcaactaaaa ataataaatt agatgaagaa ttgatcgaaa catttattga
       61 agaaaatctt cttaaacaaa tatggggaga aagtattgtg aattgcttaa aactggctag
      121 caatagtgat tatcgtcaat tcgacaactg gtacaaaaaa ttcaaatatg cgattagatc
      181 tgctgaaaaa gaacaaaaaa ttcaactgaa aattatctat gaaatttgta ataacaagta
      241 ttttgtagat catgttagag aacagctatc aatgacttta cgtgacttga tacgaagagc
      301 aaaaacagat catcgtatca aacaaaaaga cgattatatc tttgcttctc taaaaaataa
      361 agcgttagaa ctaattgaat tgcaaatcag cgaaggaata gataagcaat aagaatagaa
      421 ttgagtcaaa tgaaatggag gggtatctcc acttcatttg agaactttga atataggagg
      481 ggttattttg attaaaaatc gtttaataga tcaagtttgg tgtctagatc aaaatacaga
      541 tagtctcgaa ttaatcgaga ctatttgctt caacactata gttctcgatt taagagctga
      601 aaatgataat tgtgtaactc atgtcataat taatcaaaaa atggctcaga agctctctga
      661 gagcctcaga aaatgggcta agggaggtaa cgatgaaaat aattgaaaaa aagattgcca
      721 ataaaaaaat aaattggttg gttgttaatt ttcttgttct gatagctatg attattttta
      781 caagtaatat tgctcatgcg agtattttca attggtttga tgacacaaaa agtcaaacag
      841 agtttttgaa taattctctt tatagacctt acctaaaaaa gtacaatgga gatttcctta
      901 gtcagttcgg gatttggctc ggatgggctg tggtaaaagg catgtttact gtgacagatt
      961 cgattcaaaa tatgattcca gatgtgctgg atttgttcaa tttcattgaa agtacagggc
     1021 taaataatgt ttatcaatca gccgtaaata caattgtcat tggattaatg atcctttcat
     1081 tgatgtttgt tggttacaaa atgattactg gaaaaggaac agtggattta aaatcggttg
     1141 gaatgaacgt agtgatgtct atagcattaa tacttttaat gccaaccatg atttctagtg
     1201 gtattcaatt tgctaaaaca ttttataagg actctactac gattacaaat tcagatgacg
     1261 gagtcgcttg gtcattgata aaacaaggag taacagattt agcatatatt aataaaacag
     1321 atcagtactc aagcattgat aaaacggagg atcgaaataa tttaacacga aaaaattttc
     1381 aacagacgga tctcacacaa gtgttaacgg ataaagtgat tgataaattg gaaaaagaaa
     1441 atccaattgc ggacaatctt cgttatgaat tagttgaaaa ttctaataat gaatttgttg
     1501 ctacaaagtt ttctgacaac ttcttatcaa cattttctga tagcttgaaa tcaggctatt
     1561 accgatacca agcaaatcta tgggggattt caattgggtt gacagcgtta gcaatcgcct
     1621 atgtgttcag cgcgtttgtg atcattacag caatactaga acttgcattt aaacgtgtgt
     1681 taggtgttct tgtttttgca acagatattg agacaggaca gcgttcaaaa gttgttctat
     1741 cagatattct acaatgttac ttaactgttg ggtttcaagg atttgggtta tcaatgtttg
     1801 ctatgtttat caattttctg aactcaggac aaggtatctc aacaaatatt tttataaaaa
     1861 caattgctta tatctgtgcc gtttttgttt taatcaaagg cagtggaaca gtcatgagat
     1921 actttggtgt tgatattgga ctcaaagaag gctatgggca acttgcttct gcttttggaa
     1981 tgggcgcaat gctgtttaga aaaggatcaa atggatttaa tcgtgctaaa ggaagcggag
     2041 aaggagaaga tcgaaaacca gaaaagaatt tcggcgaaac tctatcaaaa aaagctggca
     2101 aaactggaag agctttggga tatgcccatg aacgtggatt atccggttta gcttcagatg
     2161 gagctacaat ggctagtgaa agagcaacaa aaccatttaa gagtatgcgt gatatagcta
     2221 atgataccaa gaataagttt aaagaaggtt tagatgatgg tacagtgtcc gctattaata
     2281 aaaatagcaa acctatgtta gctaaaaata aagaagatga aactggaaaa tatgctgatt
     2341 ccgtgccaac acgtctttca gatagaaaag atggtgctaa agttgaaaat gccgatagaa
     2401 ttatgtctag ttctgaacgt atgcgtgaag caatgaaaaa taatgctgaa tcgaataata
     2461 atcctgcaag cgcaattcag caaaaagtcc aacaagatat tgaagaacgt aaaaatgcta
     2521 tgcacggtca agctaagtct gctgaagaat taattaatca gaagcgtcat gaagcgaaat
     2581 acacgcctga agcaatgaat cgcgaggaaa taatgttaag gaaacgtgta gaagggcgga
     2641 caggcgctaa tattgacgga aaagaagcgt tgaccaaaga aagaattcaa gaagctaaaa
     2701 attcaaatac aggattagaa aaattagtca aagaaaatcc acaaaattct acaagtagtc
     2761 atggtggccg atcagtagat gtccgagaaa atattcaagg ttcactgaat ggtcatgttg
     2821 gacggaatgt tgatgtaaga gagaatttac aaagttcttc aaatgctcaa ggtactaaga
     2881 caattgatgt tcgagaaaat ctgcaaagta gcagtacaag tcaaccaaaa acagtagatg
     2941 ttcgagaaaa tgtacaaaat tctacaagta gtcaagggtt taaatcagtg gatgtccgag
     3001 agaatgtgca aggtagcagt gcaagtcaac caaaaacagt agatgtccga gaaaatgtac
     3061 aaagttctac aagtagtcaa ggattcaaaa cagtagatgt tcgggagaac attcaaaaag
     3121 atagtggaga aatgaaagaa aaaacacaaa aaatcaatat cgttgaagac agaaagtcag
     3181 cgaaaaaatt tgattcaaat catgaaacag tgattattga tagtgaaatc cgtgaaaatg
     3241 ataagggatc aaaatcaaga cgaagattca cttatgaaga caatgagtta ttcagagata
     3301 cacttaatga tcctaatcca cttttcgata atttattaag aaataaaagg gggggattgt
     3361 catgcaatta attctaaaga ttagtacgca tgatttctta gaacttgacg aattatgtga
     3421 atcagtaaag aacgcaacaa atgaaaaaat cacggtcact aattatattg aactgatgat
     3481 tcaagaagaa ttaaagatga aaaatgaaat agagagcaat ttcaaatgtt gctctctaga
     3541 aaaaaatgtg aggggataat caatggatga taaacaaaaa atgtacgaaa aaaatcgcaa
     3601 acatagtgag gccttaaatt ttcgtgtcga taaaaagact tatcttgaac tgacattgat
     3661 tgcaaaacaa aggggaattt ctagagcaga cgtttgccgt gagcggttaa agggactcaa
     3721 atatgaaccg ccgttgttag ctgaagatga tagggtagtt ttaaccaaac agttaaatca
     3781 aattggtaac aatttgaatc aagcggtaaa ggcattaaat atcattgcta aatattttag
     3841 agaagaatct gaagataaaa aagaaaagag cctacatttt agttttgatg ctagccgtta
     3901 caacattttt aaagatgaac ttgcgaatag catagcaaaa aagttgatga ttagttatcc
     3961 agatgacatt tttttggagt ccaatcggag tgccccctct ttttttaaat tatcagaagt
     4021 agaaaaagaa tatttcgaat attggataga aaagcaaact agaccgggta cgaaagaaga
     4081 gtattcccaa aaagttgatc ggaatttaat tcactcaatt cagattattg caagtttaaa
     4141 agatgaggtg aaacaacttt gggagctact ataacatcga aaggacgcaa gaaaaattta
     4201 tcctcgctat tacaatatgc agctaatcct tcaaaaaaag aaaaatgtgt tgcagtctca
     4261 ggaataaatt gtagtgatga tagtgaggaa gcactgcaag aaatgaattt agtacgtacc
     4321 aagatgaata aatctaaaga tatagcacaa gcgtttcatg ttattcattc gtttgataag
     4381 agtacttcta aagagttaag tattgataaa atgcacgaaa tagctgtcga atttgcagag
     4441 aaagcttttc caaattcgca aatcattgtt gcaagtcaca atgataaaga tcattttcat
     4501 agtcatcttg tcattaataa tattaacatg gaaacatgta aaaggttgcg tattgaccct
     4561 aaagatttag aactaatgcg aaaaattaac gatcaagtcc tcaatgaaca tggtcttgaa
     4621 tcagtgaata atgagtacta tgaaaactta gaaaagagaa ccaatcttta ttcaaacgat
     4681 ccttctaaga tgaaatatgg aaaaattaat tatcaagagt ttgtgcaaaa tgcgatcgaa
     4741 gcaacgttgg aaaaatgcga agtgaattct tttgaagatt tttcggatca tttggcaaaa
     4801 gaatataaca ttgaagttta tcaattttca aaaacaaata acaaacttgg ctatgttctt
     4861 tacaaacaag gtgtaaattt ctatgagcgt agaggtgagt taattacttt aggaagagat
     4921 aaacaatcta gaaaagaaaa aagtcagtat gtcgaacgta cttttagtgc aagaaagtta
     4981 gggaaaagat attcatttga atctgtttct gaacaacttg aaaagaattt agaagaatca
     5041 catataaaaa atttttttaa tatggaaaga gagcaagtta tgtcgctaat tagtggagta
     5101 aattacaaaa ctggtgagtt aaatgtaccg tttgtggaat tagattccat cgcacaaaag
     5161 caatttagcg accgtgaagg cgatgaaaaa ttcattaaat atgatgactt cttgcgagag
     5221 gtagacaagc attttgtaaa tacaatgtct ttagaagatg atcaagaata cacgattaaa
     5281 cttcatacgg atgtgaatcc tcaaacttta cgtagtgcga aaagtaaaac aatttctatt
     5341 aaaaagactg atcagaaaga gttatttcta ccggcaaagg gttttgaaaa ttgttctcat
     5401 acaccttctg ctaaacagat tcactatcaa atcagtcgca taatgaatga cgatgtaaaa
     5461 atgtatcgtg ctaaaaagga ttatgaattc acacaacaaa tgattgaaaa tcagcaaaaa
     5521 aggcaacaaa attttgaaat ggaattctga atgggcttgt tagtggactt tccagttttt
     5581 tgcctatgaa gagttgcttg gaaagaagca gtttttagga catacgccag ctacccatct
     5641 acttgccagt caatccgccc cgctcacgct aggctacttt ctggcaagtc agatggcggg
     5701 taaatctctg agggagcaag ctccctacag actccctccc aaaaaaagaa aggtggaaga
     5761 aaaaaatgaa ctatccatat tttaaagtct cggcttcaga agaaactaaa gaaatattca
     5821 acaattttta caaccaaaac aaaggaattt ttggttcaaa ggcaaatatg tttcgtgtta
     5881 tggtttcaaa tttaccagtg ttagctagtc catcaaataa taaatttaat gatcctgaaa
     5941 gcattaaatt tgaacaaaaa atttcagaac tagaatcaat gataagtaat gaagtaattg
     6001 aaaagctgga tgacattgat caaaagttat cgtattctct aaaaaacaaa tacaaaacgg
     6061 aggagaaaaa agatgtttaa agtttacttc ccgtttcagt ggctcgttaa aggaattctt
     6121 ttagtgctac taggattgat tctttttgga agttatctaa caattaaagc cagacatttt
     6181 ccagaagaaa agccaataaa aattgtaaca aaagatttaa aaataggtga aacttatcag
     6241 gcagatgggg ttgaactaac atttaaaaat attgaagtga ccgaaaaaag aaatgaattt
     6301 gcacaacatg atcgtgtatt acaagtgacc tatacactta taaatgatac agaaaatccg
     6361 atacgtccac aacaatggtc gattgaaaat aaggatggtg aagaacttta tgagtatgat
     6421 atgatattgg ataatccaga gtatattgaa agtggcaaag aagaaacagt aaattactat
     6481 tatgaattac cgattgaaag gcaggactac caaatcgtag tacgtagaga gaatggttac
     6541 atagttgggt tgccatttac tttataagtt ttattttgtt agtagttttt aaggtaatat
     6601 cagttttgtg atttttgaaa atggaactgg gagaatggct gaggaaaata aagttgttcc
     6661 gtttgaaaca ttagtgataa ataaggaaga gtttgagcaa ctttttcttg atttttttat
     6721 tcaatatgac atgatttatg attttagttc gtcactagat ttatttgaat tagaagcaga
     6781 aataatagaa aacagtcgag agaagaatgg taaaatcatt atttcagtag aaaaattcaa
     6841 tgaattattc tcacaaacat ttgaaataga tccagactat tatcaaccac aagaagtttt
     6901 tcgagagttt agaaataaga ttattggttt tagtcaaact acaaaatgaa agcttaataa
     6961 agcaagtgtt gatagaatta ttcctcaacg cttgctttat tttatgggca cttcataata
     7021 gaggtataga gaagagaggt attcattttg gatactatta ctccaacaaa cttcaaagaa
     7081 aatataggta aagtgttgca agaagtagtt cattcaaatt ctgaaataga aattactagg
     7141 gattctaaaa atggattgaa cgatggcgtc attatgattt ctaagagaaa atgggagttg
     7201 ttacaagaag aactttattt acaacgaacg ggtacatggg cttatgtatc tgattta
//
LOCUS       DANKMN010000102         6869 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000102 DANKMN010000000
VERSION     DANKMN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..227
                     /locus_tag="PW224_002640"
     CDS             <1..227
                     /locus_tag="PW224_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDL2909269.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..574
                     /locus_tag="PW224_002641"
     CDS             227..574
                     /locus_tag="PW224_002641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909270.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            571..1620
                     /locus_tag="PW224_002642"
     CDS             571..1620
                     /locus_tag="PW224_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2909271.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1635..1841
                     /locus_tag="PW224_002643"
     CDS             1635..1841
                     /locus_tag="PW224_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909272.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="PW224_002644"
     CDS             1862..2458
                     /locus_tag="PW224_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HDL2909273.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3410..4009
                     /locus_tag="PW224_002645"
     CDS             3410..4009
                     /locus_tag="PW224_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2909274.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4058..4774
                     /locus_tag="PW224_002646"
     CDS             4058..4774
                     /locus_tag="PW224_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL2909275.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            4788..5567
                     /locus_tag="PW224_002647"
     CDS             4788..5567
                     /locus_tag="PW224_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDL2909276.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5650..6006
                     /locus_tag="PW224_002648"
     CDS             5650..6006
                     /locus_tag="PW224_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2909277.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            5996..6262
                     /locus_tag="PW224_002649"
     CDS             5996..6262
                     /locus_tag="PW224_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2909278.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6421..6699
                     /locus_tag="PW224_002650"
     CDS             6421..6699
                     /locus_tag="PW224_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909279.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(6696..>6869)
                     /locus_tag="PW224_002651"
     CDS             complement(6696..>6869)
                     /locus_tag="PW224_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2909280.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT         2527 a          957 c         1333 g         2052 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttggatccat
     3061 aacatgacta aattctgaat actttttaaa gcttatcatt tggtaaaact gaatataagt
     3121 taattatata gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa
     3181 attttttaaa ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa
     3241 agtccaatgt gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg
     3301 ctctaatagg aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac
     3361 ttattatctg ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt
     3421 ggaaccgatt cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa
     3481 atatgggcaa cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc
     3541 cgatttaaga cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt
     3601 caaagaacgt ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc
     3661 gaacaaattg cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca
     3721 agcacaagac ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac
     3781 ccgacaaagc ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat
     3841 tggtccccat tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac
     3901 agagatcatt cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg
     3961 aatcacccag gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc
     4021 cctatataat agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa
     4081 ctttcctgat aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat
     4141 tgtagaactg gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac
     4201 agaagggatt attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt
     4261 cgttaactta aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga
     4321 gacaagtaag cggattaatc ggatcgttgc agcagaggat gcgctttttc ccggtgaaat
     4381 acgaaccggt ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac
     4441 tgaagacgag gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga
     4501 aaaagccctg cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt
     4561 gggtacagcc ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc
     4621 aatacccgaa aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa
     4681 tctattagtc aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat
     4741 taattatttg aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag
     4801 acgaagcaaa acaattagca acttattttt cagttggttc agcgggatat attttagact
     4861 ttactaatgt aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc
     4921 attatggggg ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg
     4981 attcgttaaa gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat
     5041 atgaatctac tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag
     5101 atacttcaac tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc
     5161 ggcagctacc cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga
     5221 caaaagcaaa tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac
     5281 cgagtgttaa tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc
     5341 tcaatattaa aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca
     5401 atacattatc agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg
     5461 gctcaaatag aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg
     5521 ttttgtgcga gtatgtgttt actaagtata aaggattaaa tagataataa aatgcatatt
     5581 ttatagtata caattcgaat ttttatgtta tatactataa tataaaatca atatatgata
     5641 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
     5701 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
     5761 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
     5821 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
     5881 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
     5941 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
     6001 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
     6061 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
     6121 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
     6181 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
     6241 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
     6301 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
     6361 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
     6421 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
     6481 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
     6541 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
     6601 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     6661 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     6721 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     6781 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     6841 aatttatgtc gacgttttat cgatcgatg
//
LOCUS       DANKMN010000103         6676 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000103 DANKMN010000000
VERSION     DANKMN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            127..579
                     /locus_tag="PW224_002652"
     CDS             127..579
                     /locus_tag="PW224_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909281.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="PW224_002653"
     CDS             593..1282
                     /locus_tag="PW224_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909282.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="PW224_002654"
     CDS             1407..3758
                     /locus_tag="PW224_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL2909283.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="PW224_002655"
     CDS             3838..4233
                     /locus_tag="PW224_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909284.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFKPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="PW224_002656"
     CDS             4558..5175
                     /locus_tag="PW224_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2909285.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="PW224_002657"
     CDS             5216..5515
                     /locus_tag="PW224_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL2909286.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="PW224_002658"
     CDS             5549..5716
                     /locus_tag="PW224_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2909287.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="PW224_002659"
     CDS             complement(5761..6366)
                     /locus_tag="PW224_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2909288.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2296 a          935 c         1303 g         2142 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaacct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt tggttctgtt gcgaagtttg aaaatcaaac
     6601 gcgccctctc tgaactccaa atatcttagg ctgttattcc cattaatacc ttgatttcag
     6661 tagacaccga aaagcc
//
LOCUS       DANKMN010000104         6517 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000104 DANKMN010000000
VERSION     DANKMN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(53..826)
                     /locus_tag="PW224_002660"
                     /pseudo
     CDS             complement(53..826)
                     /locus_tag="PW224_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            847..2049
                     /locus_tag="PW224_002661"
     CDS             847..2049
                     /locus_tag="PW224_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HDL2909289.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            2782..3750
                     /locus_tag="PW224_002662"
     CDS             2782..3750
                     /locus_tag="PW224_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2909290.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3761..4576
                     /locus_tag="PW224_002663"
     CDS             3761..4576
                     /locus_tag="PW224_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDL2909291.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4644..5354
                     /locus_tag="PW224_002664"
     CDS             4644..5354
                     /locus_tag="PW224_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2909292.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            5390..>6517
                     /locus_tag="PW224_002665"
     CDS             5390..>6517
                     /locus_tag="PW224_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2909293.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2196 a         1001 c         1329 g         1991 t
ORIGIN      
        1 gccaataaaa cttagtccga tatgcggcgc gttctgtgcg ccgcatattt ttttagtttt
       61 caaagatcag accttgttgt aaatagatcc gtgcccgaaa gttataaaag ttgcgataac
      121 caaacgcaat tcgtttaatc agcttgatct tgttatacag cccctcagtg acgccgtttg
      181 agtgggacac cttaaaggca tttgaaatac ccgattggta gcgatggaag atcgtgaatt
      241 tctttttaaa cgactgaggt aacgtagatg aaattgtttt aagggtttct tgaaagccta
      301 ccgtatctcg tttaacgaaa taatagcgta ataattgaca aaaatgatac gcagttcgca
      361 attcttcgtt gtagcttaac agctcattaa cgatttctgt ttgactaatt ggtcgtttaa
      421 acagtcgatg ataatgccgt ttgagtgcct cccatgtcgt ggaatctttg agtaagagtt
      481 tccaatatcg cttgagccga cggtacttct tctgattttc agagtgtgag gttcgaaaac
      541 gattcatgat ctggacacgt aattgattga agctgcgatt gatgtgttgg acaatatgaa
      601 agcggtcagt tacgatctcc gcatttggaa agaccgtttt aagcaactga caatagctgg
      661 cattcatgtc catcactagg tacttgacct ttaaacgggc ctttctagga aagcgcagga
      721 agtattgagt tagtttgtaa agtctgcgat cttcaagggt cgtaaatagc tgattggttg
      781 tcccatcaac acaaataaaa ctcatggaac cagaagtggc aacattgtgt acttttcttt
      841 gcaatcttgt gtacaaaact attgcaaaac acagcgcata agaatcctta caaaacagta
      901 gatgaagcaa cccaattaga tcttgttata gttgttaatc agctcttaac gggttacgat
      961 tcaaaatggg tcaacaccct atacttagat aagtcacttg aatatcagaa cataattcag
     1021 gctttttcac gcacaaatcg tcttaatggt cctgaaaaac cgttcggaac cattcgttat
     1081 tatcgtaaag cacatacgat ggaacagaac attgaagagg ctattgagtt atactcaggc
     1141 aaacaagcaa aagggttatt tgttgaaaaa ttatataata acttagaaaa gttgaacgat
     1201 atattcgaag aaattaagtc cgtatttaat atttctggca tagaaaactt cagtcattta
     1261 ccggaggttc caggggaaat taagcaattt gtaaatcttt ataatcaact aatccattgt
     1321 tatgaatcag cgatgataca gggattcaca tgggcaaaat cgacttatga aatccaggat
     1381 tatgaaccta taacagtagt aatcacagaa tatgaattta atatcttggc acaacgttac
     1441 agcgagatag agagagaacc tagagacgga aatgatagta tagcgtatga cattgattac
     1501 caattacctg aaggtagtcg tagtcgtatt aattatgagt atttaaacac aaacttcaaa
     1561 aaatacatga atataaaaga agaacgtgat tcgaatttaa ttcaatcatt gttaaacgac
     1621 ttacatcgca catttgcgca attaccagtg gagttacagc catatgctga gatgattcta
     1681 aatgatgtaa ataatgggga attagttatc aaagaaggat gggaatttac tgattacctc
     1741 aatgaatatc agcttagtga agaggacgat tttatccaag atttagtgga ttcaaccaat
     1801 attaatgagg cattattacg tgagatgttg gatttacgct taaccgaggt aaacattaat
     1861 gaatatagtc gatttgataa attaaaatca agtgttaacg taggtcattt cagtggatat
     1921 atcgagaaaa atttaggtaa aatggtaata ccaatcaagg taaatatgct aattgacaga
     1981 ttattgcgtg catttttatt agaagatgta ttcgatgtta cagagtacat aaagaattac
     2041 tttaactaga gtacacctac atcttatagt acataaaaaa attcatcttt tttagtttaa
     2101 taattcacca aaaaggtgag taatgccatt tttgaaaatt gagcttgttt tatgtctaca
     2161 tctgtatccg tatataatcc aaaggattat aaactcagaa gagtctttat tttttctgaa
     2221 tataactact gtatcaaaaa caatcgatta tgagacaaaa caaaatgacc tcttttattt
     2281 aagaaaggag gtcatttttt gtatcataaa ttgtattaaa atcaatgtat cactttttaa
     2341 tcaattttaa gaccataaat tgcgtactaa aagaaattct gcaattcaaa ataaagcatt
     2401 ttaagccatt ttcaaattta agtgcaccca tctacccgtc aaatcacttt attttccact
     2461 ccagtagttt cctaattcaa ctgtgtgcaa aacgatttct tctaaacaaa tcaagacaaa
     2521 cttataaacc ttcatcccag caccataatt atttaaatca taggcatttt tcgaaattag
     2581 tatatgtact caagtttcac tgcagattat ttatattttt tagagaaata aatttttcct
     2641 attgacaaac acaaagaaag cggatacaat acaattatgt cacatgacgt tatatgacgt
     2701 tatgtaatat ctttacattt aatcagttac ttttattttg gataaaatta acatttttaa
     2761 aattttaagg aggaaatgtt gatggatgta gcaaaaacgg ttaacgcaat tgtagaaaaa
     2821 aatgatggag agatttctgg aatttatttg gtggcttgtg gcggttcgct ggtagatatg
     2881 tatgtctcag attattttct taaagccgaa gcatcgaagt gtattacagg cttatataca
     2941 gcgaacgaat ttaaccatgt aaccccaaag caattagatg ataaatctgt tgtcattcta
     3001 tgttctcatg gaggaaatac agcagaaacg gtaaaagcag gagaagtcgc tcaagctaga
     3061 ggagctgtga ctgttggtct gactcataat aaaactgcgg atttattaaa ggtttctgat
     3121 catagtttcc tttacgaatg gggagatgat gcaaaaatag tcaataatcc tatggccatc
     3181 attcttgatt taactgtatc tttggtcaat gttaccgaaa catttgaaca ttacggtaaa
     3241 ttcagagaag gcatgaaggt tatcgatacc gtaattgaaa atggcattaa gcaggtgcag
     3301 gatcgtatta aagtgtttgc tgataagtac aaagatgaaa caatgtttta tatcttaggt
     3361 agtggaccat catatggaca tgcttatggt ttttcaattt gctctttgat ggaaatgcaa
     3421 tggcttgatt caacttctgt tcattctgga gaattcttcc atggaccttt tgaagtaaca
     3481 gacaaagata cgaattttat tctcttgatg agcattggac gcactcgtgc tttagatgaa
     3541 cgggcaaaac gattccttga taaatatgca gaaagagtag aaatcgttga tgcaaaggaa
     3601 ctaggattgg atatgatatc agatgaagtt tctgaattct tcaatcccat tttattctac
     3661 agtattcttt cagaatatcg ttcagcttta gcagatgcac gcaatcatga tttagatgtt
     3721 cgtcgctata tggggaaagt ggagtactag gaggattaaa atgaaggtat taggcttagg
     3781 tgataacgtc gtcgataaat atgaaaatct gggaattatg tatcctggag gaaattcatt
     3841 gaattttgca gtttttgcaa aaaaactggg acatgaaagt agctttatgg gagtttttgg
     3901 ctcagatgag gaggcagctc atgttttatc agcaataaaa aaaataggac tggacaacag
     3961 tcactctcgc tttgaaaaag gcgagaatgg ctgtgccaga gtgaagattg atgagggcga
     4021 ccggattttc cttggcagca attcgggagg tgttactcgt gaatacccga ttcagcttac
     4081 tgaagaagac agagaatatt taaaccagtt tgaattgatt catatgggat tatatagcca
     4141 tgtcaatcat ctattagaag agttacaaaa tgtttctgga aagatttctt atgatttttc
     4201 tgacgacttt acggaagaag aggttcagcg ggcaattgat aaagttgatt ttggcttctt
     4261 ttctatagaa gacttttccg atgaagaagt aaagcagttt ttgatgaagc atttttgtga
     4321 gcgaaatgat gtattgattg caacccgagg agaaaaaacg gctattggtt atgatggagc
     4381 aaagttttat gaagtgaacc ccgtgctaga agaaccagtt gatacaatgg cagcgggaga
     4441 ttctttctta acagctttcc tactttattt tctcgaaggg gacgatatag tcaccgcgat
     4501 gaaaaagggg aatgaatttg ctgggaagtc ttgtctggtt gacggttcgt tcggctttgg
     4561 tattcattac gattgagctt gttttaaatt cctgcatttg ataacctaaa gaaagaaatc
     4621 ccggtaaagt ggaggttaaa aaaatgctgg atacaaatgc acaattacca ttgtacttac
     4681 aattaaaaga aatattaaaa aataaaatca atacagggga gtatcccgag aatacgaaaa
     4741 ttcccgctga atcagagctg atagaaagtt atgaggtcag ccgaatcaca gtgagaaaag
     4801 cagtagaaga actggtagaa gaagggtacc tacttaaaaa acaagggaaa ggaacttttg
     4861 tcaaccggca taaagttttt cgtaaaattg aatatgttat gggtttcagt gagagttgta
     4921 aatctaatgg ttttacaccg actagcattg ttttggaacg caagatcatt ccagcaactg
     4981 aagggatggc aaaaaaactc gatatcgctc aaggtgatga tgttttatat atccagagaa
     5041 agcgaatggc ggataacatc cccattatgt tagaaaacaa ttatttttct tatgaaagat
     5101 atagtttctt aatggaggaa gagctgactg attctttgta tgaagtactt aaagtccaag
     5161 gtaccttagc catcaatcct ggggaaactg cacttgaaat tgtcacagct gatgaccaaa
     5221 ttgccaaaat aatggaagta gctgttggga cgcccttttt ctttatggat actttaatca
     5281 acgatcaaaa caacatacca attcatgtag gtaatcaata ttacctggga gaagtatatc
     5341 gtttttactt atgatttcct gttttgattt caaggagtag attttattta tgaagcaagt
     5401 attgcggtca aaaatgattt ttgatggaga gcagccacct ttcaaaggtt atattgtaat
     5461 caatgaaggg atcattgaaa aggtggaaaa caactggaat tacagtgatc tattaaatga
     5521 aaacacggaa ttaattgctt atgatgatca attcgttatg ccagcaattc atgataatca
     5581 tgtatttttc agtggttatt tggctatgaa tgctggcata gatttaagcg atacatcttc
     5641 agctaacgag gctgtagagc gtattaaagc tgtaatgaac cataaaaaag cagatgaacc
     5701 aatttatgct catggctggg atgctgccct gtggaaaact gacccatcag aagagctatt
     5761 gaatgaaata aaccactttg gtccaatcac agcgatcgac aaaaatcgca gccattgttg
     5821 gatgaatgct attgctaagc agacttatgg ttttacagaa aaagaaacca gtgctgaaag
     5881 tcgagtatta ttaattaagg aaatgctagc aaacaaaaag cttttagcag aggtttacgc
     5941 tgaatttgaa gcattactgc tttcaaaagg tgttatatca atgaaagaaa tcgtttttga
     6001 tgacgcagaa tttctttcta atatacctca gaggaaaata cttagtaacc tatatgtgca
     6061 ggcagtccaa gaacctctac agcatgaact tctatatgag tataaggaaa aagttttccc
     6121 tgaaaaggtc cgctttggtg gagtgaagat catggtagat ggtgttgttg cggatagaag
     6181 tggagatatt tacggtgttt atcagtcagg agtagcttca cccgagattg actatgaggc
     6241 aattaaagca gaagttgctg cattcaatga agtagagatt ccttgctgtt tgacaacgga
     6301 aggagataaa gcgggttata aagctgcgtc aattcttgtc gaatttggaa agcggttacc
     6361 tgaaggcgtt tttaattcga ttagtgattt ggaaatggtt actcaaaaga cagcagaaaa
     6421 aatgaacgaa ggacaagtag tagcagaggt atatccacaa attctcggct tgaatcaaac
     6481 gttagaagag gcttacatgt caactgttat aaaaaat
//
LOCUS       DANKMN010000105         6387 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000105 DANKMN010000000
VERSION     DANKMN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            330..1175
                     /locus_tag="PW224_002666"
     CDS             330..1175
                     /locus_tag="PW224_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2909294.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="PW224_002667"
     CDS             1456..2676
                     /locus_tag="PW224_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL2909295.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="PW224_002668"
     CDS             2673..5393
                     /locus_tag="PW224_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2909296.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="PW224_002669"
     CDS             5377..6321
                     /locus_tag="PW224_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2909297.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          987 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DANKMN010000106         6372 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000106 DANKMN010000000
VERSION     DANKMN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(59..472)
                     /locus_tag="PW224_002670"
     CDS             complement(59..472)
                     /locus_tag="PW224_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2909298.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            complement(548..844)
                     /locus_tag="PW224_002671"
     CDS             complement(548..844)
                     /locus_tag="PW224_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909299.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            complement(844..1050)
                     /locus_tag="PW224_002672"
     CDS             complement(844..1050)
                     /locus_tag="PW224_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909300.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            complement(1047..1244)
                     /locus_tag="PW224_002673"
     CDS             complement(1047..1244)
                     /locus_tag="PW224_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL2909301.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            complement(1268..1573)
                     /locus_tag="PW224_002674"
     CDS             complement(1268..1573)
                     /locus_tag="PW224_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909302.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(1570..1743)
                     /locus_tag="PW224_002675"
     CDS             complement(1570..1743)
                     /locus_tag="PW224_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909303.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            complement(1740..2309)
                     /locus_tag="PW224_002676"
     CDS             complement(1740..2309)
                     /locus_tag="PW224_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL2909304.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            complement(2455..2790)
                     /locus_tag="PW224_002677"
     CDS             complement(2455..2790)
                     /locus_tag="PW224_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909305.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            complement(2787..3173)
                     /locus_tag="PW224_002678"
     CDS             complement(2787..3173)
                     /locus_tag="PW224_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909306.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            complement(3170..3487)
                     /locus_tag="PW224_002679"
     CDS             complement(3170..3487)
                     /locus_tag="PW224_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL2909307.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            complement(3487..3792)
                     /locus_tag="PW224_002680"
     CDS             complement(3487..3792)
                     /locus_tag="PW224_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909308.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            complement(3789..3950)
                     /locus_tag="PW224_002681"
     CDS             complement(3789..3950)
                     /locus_tag="PW224_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2909309.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(3965..4324)
                     /locus_tag="PW224_002682"
     CDS             complement(3965..4324)
                     /locus_tag="PW224_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL2909310.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(4321..5172)
                     /locus_tag="PW224_002683"
     CDS             complement(4321..5172)
                     /locus_tag="PW224_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2909311.1"
                     /translation="MESLASTMEKLIRRVLVQSGRCPECSEPLYSWRGKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(5189..6019)
                     /locus_tag="PW224_002684"
     CDS             complement(5189..6019)
                     /locus_tag="PW224_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL2909312.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLGSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDIDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(6022..>6372)
                     /locus_tag="PW224_002685"
     CDS             complement(6022..>6372)
                     /locus_tag="PW224_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909313.1"
                     /translation="NVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCI
                     CGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGI
                     KLNQETIKKLRIGG"
BASE COUNT         1585 a         1465 c          954 g         2368 t
ORIGIN      
        1 cataatatca ctggtaaata gctaaaaacc gccatcattc cgccaaaaaa ccgccaaatt
       61 atttataagc aattattctt ccgtgtttat atgcttctgc aaactctatt agagcttccg
      121 acttcatccg ttgtatactt ctttctgaat aacccacttc acggctaatt ctgtagtttg
      181 agaagctatc tggcacacaa aagctgtagt agagtatctg acggctaatc agactaagag
      241 ccatcaaagc cgctaaaatc gcatctctct ctgcttctat atccatcatc tgaatgatcg
      301 cgtcctctgc cttattgccg tgcttcggcg ccttcggcat atccgtaata attggagact
      361 taatatctat caaagagcga cctgccatcc gctccaaacg ccgaaagttc ttcagcacat
      421 ctctcgcatt acatcttgtc tgtttgaaat ctacctctcg taacaattgc atcaagtcaa
      481 accgctcctt tatgtgatat aataaacttg tctgttttat tatgttagtc ggagcgatcc
      541 ggctttttta tttgctctcg attacttcca tgtcaactaa tctcgctaca gctaaattct
      601 ctttgctttt cgctaaccac ttgtcacatt ccatcgtgtt ttcaatacga atgattgctg
      661 agttcgacaa taccttttct acatatccac gaaatggata gataaatcct tctgcttcac
      721 agcgaaccat gtcaccgact ttgaattttg gtttctcacg tgttttaggg ttctttgtcg
      781 gcatatctag catcaaaccg ccgataccat gactactagc gtaaaatccg tcttttagtt
      841 tcattactct accccctcat gatcgacagt gaccagttca tatacttgcg ttaatccacc
      901 aagtcgtcgt gaaacttggt tagcttcttc gagattatca aaccatcttg cgctttggag
      961 tatgtctaca atggataatg tgttagcccc tatagaacgt tcatcgcttc tgtaaaattg
     1021 atttccaaac tttactacat aaatcttcat tctgtatcct cccatttaat atctagtatc
     1081 gaaattccaa acttacgaat agcatcactt gcatcagcaa cacactgact tgccacttta
     1141 tatgtttctt ctgcagaaac accatactcc cgttcgaact ttactttcgc tacattcagt
     1201 tcttgatttc ttagttttgc tactctgcgg tgtctgttgt tcatttttct tcacctctgt
     1261 tttgttatca ccatttcttt gttgaaaatc cacaacgtct atactgttta attcgtttct
     1321 taggcctttc tagatcaatc agaataaatc tgtcggatat atgataatct tctattacag
     1381 ccgtatacat tggattcgga accgagtacc atgatctttt tttatcaaac tcatccgcat
     1441 catctcttgt aatttctatc atgttagcag acattagata gccctcttct tctgcctcgg
     1501 atgggtggga atttgtatcc cagcttcgag gaaacctggc caaatctctt tttaaataaa
     1561 cttgaacatt cattccgctt cctcctcatc gatctctatc tcgacatctc tgccttctaa
     1621 aatctgtttg gtatcaatgc tttttgtttt caacgccaac ccaccgtctt tttgtgcagc
     1681 aacaatggta atcgtcattg cctttgttcc gtctggtata tggagcatta tctgtctact
     1741 catccttccg ccacctcttc cactggcaca gcaaatggcc agtatctttc atcaattgct
     1801 ttgatctgtt tttcggtcag ttggtagtgg tgtgcaaggt attgtggcag gtaagctgtt
     1861 gatactagtt taacttcatt actgcaactg atctccttga caaggtactc gccagcaatt
     1921 attacactgt acaacggctc tttctcgacc tcgtagccgt tgtacaagct caatagtgtt
     1981 tcatatgatt gtctcgttgc ccaagcgagt aattcttctc cttgttcttg cgtcactatg
     2041 ccatctcgta caaaccaatc acagaaataa tatgaatcac catctttaga tttgattaaa
     2101 tacgctattt tttcagcttt cgtaaaaggg tccgtagatt tctcaagcca accggccacg
     2161 agttgtggaa taactagctt cttcggttcg tctatttttt tcattaacgt aatacctgca
     2221 tttatactct cgttatagca agcagctagt gcatcatttc ctatcacttt aatactttct
     2281 aactttttga ttgcttcctg tttattcatc gctgttcctc ctaaaaaatt aactgccaaa
     2341 gcaatataat cactttcaat gatacaaatc ccaccagtgc aataattaca gatataaacg
     2401 cacagtttat gaaacagcca actaaggcgc ttgcttcttc taaccagtct ttcattactg
     2461 ttcctccttc aaccataaat ttggaaaatc atcggcatat atctctattg cgccaaataa
     2521 cttgatcgct gattttgatt ttccatgtat gtcaggactg attaaacgat cataataatc
     2581 ctgactgatt gtttttccct tgtactgata acgtgtagcc gcagtgtaat atactgctgt
     2641 cttgctcaga taaagatatt ttgctaaatc agccgatatc cgtaacaaaa tgattctatt
     2701 cttactttta attccacaat ttagcaatgc tgggacattc ccattttcat cttttaaaga
     2761 atcgatgtag tcagtagcta agataatcac tctacctcca ataactcgct attctcgtgt
     2821 atatttccgg tgacttcata ttcgtatccc atcttttggt ggctgaatat tctatattcc
     2881 attccactaa ttaatgattt acaaactaat ccggaatgaa actcggattc ttgtacaacc
     2941 gttttttcat taactaaatg atcaaaacca ttacttacat taaccagaac tacatcccct
     3001 tcaaatattt ctacgccatt cttatctttc agtcctgtgg attgcataag cacatattta
     3061 tctggagcca tttcagcatg agtgatcaac cttcctgctt gcccatattt catttcttgt
     3121 ccaattgttt tacctttaaa tggtgtgtac cacgatcgaa atctcggtat catttgctgc
     3181 cctccatgta ttcgtctaat atctctttat atttctctac aaatttgaaa cgatcttgat
     3241 gaagtttctt gctccaattt gtttgccgat ccagctcacg catttgatcg aacccttttt
     3301 gaatttcgtt gtaataaaat tcaatgtttg ctgctgcctt ccaatgcctg ctacttcgca
     3361 ctcctgcccc tgtttcagcc atttccaact taactaattc cgctcgttct tttgattttt
     3421 tgtctttctg aatcttcatc atgattttct tgaggatgat atcactgtat tgtgtaatga
     3481 gattcattat ttctcctcca catacctaaa ctgtcttcct ttagaatcaa tatccatatt
     3541 cttcgctcta tcccagatga tgtttttgct cagaccagta atttcagata actgttcagc
     3601 ggtacctgtt actagaattc ggtcaccgtg ccagattgca atttttctcg gcattctccg
     3661 tctggttttt tcagtccaca ttgatttacc gagcttttgg acttctgcaa ctatttcttt
     3721 gtcttcttgc caattctcag aatgtgtcag ttcgatgatt cgtttcattg ctgctttctt
     3781 atccacgctc attcctccaa tctacgaatt tcccttctta agttctctat gtgcaaatcg
     3841 attgcctttc tcgccgtttc attgaccatc actgcctttg ttcgttccag atcgtcaatc
     3901 tcacgttgaa tgcttcgaat tcgcatttga atcacttctt ctgttgtcat gatagaccac
     3961 ctctttaaaa acgctcttcc ttgaacgtat tccgatattt tttagctaat atcaacggca
     4021 cttgatattg atgacaaaac agctttgcct tgatcttaaa gtcttttgtc tgcattcctt
     4081 taacatctac gactttgaca agtttgccgt ttttataaaa tgtgaagtcg ggaatatact
     4141 cgatcttgcg atacttcttt ccgtctagtt caaatttcgg catcagctca aatctttcct
     4201 gaagttttac tttccagcca ttcgcttcag cttgccacaa ggctagatcg taatactctg
     4261 cttctgcgat agaatcaaac ttgatacctc gatgaacagt ttttttatta cggtatttat
     4321 tcatgcgata ctacctttca ctggttttat gcgcttgtct gctgtttgct ggaatttcag
     4381 cgcataacct tctgaattct taaatatcct agaaacaatt ctttcaccgt aggcttctct
     4441 tagttcagga ccagataagt ttgttgtgat gatcgttgcc ttgttctgtc ttgcttctaa
     4501 gagcgtgttt aacgtgttgt ttgtaaactg cctactattt gataccccac tacctaattc
     4561 agctccaata tcgtcaaaaa ccaccaaatc agttgtttta atatcggcta taagcgatcc
     4621 ttcaatttct tttctcagtt cagcattgtt ataagaaaac tttatttgct ctaataattc
     4681 ttgatagctt ataaaaagta ttttcttgtc ataatttgag cgctcaagta tttcccaagc
     4741 tgtagccatt gataagtggc ttttcccgct tcctgatttc cctgatagaa tgaaatgtgc
     4801 aggatggttc agcagaacct cattcgtata gctttttgct ttttctaaag cgatttttgt
     4861 ttcttgatcc actacgtgat aattttccat tttgcattta aacaaagttt tatctgttaa
     4921 taccgaacca ttttgaaaaa aactcaacgc tcgtgctttt aagctgtcgt tatatatccg
     4981 ttcggtctgt atatcctctt tcacacgtaa cgctttataa ccacaactca tgcatgttgg
     5041 tttacaacgt tctgacccgt ccttattttt ccctcgccaa ctatacaaag gttcgctgca
     5101 ctctgggcat cttccacttt gtactaaaac tcttcttatt agcttttcca tagtacttgc
     5161 taggctttcc atgtgatgca tctccttttt aaattggcaa gtcgtcatat tcactaggat
     5221 tgctgtactg taatttttga ctttgctttt tatgattatt tttatctgcc ttgatttcga
     5281 atttaagctt ctcaaatttt tctcgcagtt tcttagcact tctaatattt ccaaaccaaa
     5341 attcatttgt aggtagccaa ttgatcacat actcaatcgc ttctatagat gctttatctc
     5401 tttcttccat caacctgatt gtgtctgccc atttttcgat atctacttta ttcatttctt
     5461 ttggaaaatc ttcagttaaa ttactttgca actttttagc aaggcgtaag tgttcgtcag
     5521 aatacttacc tttcttttct tctttatcta tatctatatc ttcttctata tctttatctg
     5581 taccgtcacg tgacgtcacg ctaacgtcat tttccaattt gagacgttcc tgtctctttc
     5641 tttctctgta tttacggttt ctttcagcgt tttttagcct tactttatcc atcccttcga
     5701 tattttgatg tttttcccaa ttactgatgg caatcagtcc atcactgcct agatcaatca
     5761 tgttgaaatt tgccaatgta gttagcgcta ggcgaaccgt atttacgttt ttgccgaaca
     5821 atgtagcaag catttcttcg gtataaggca tgttcctctg gatatatatc agaccatcgt
     5881 cgttagtctt tcctgctaaa actagtaatc gaatccatat aacgatgatg gcatccgact
     5941 caggaacagc ttggattaat cgtatttttt catcgtcaaa catagtagtt ttaagtttga
     6001 tccaacttat ctcagccaaa tttatcctcc tatccttaac tttttaattg tttcctggtt
     6061 taacttgatt cctttgattt gatatttatt tttgaaattg atcacaccta ttttgtgttt
     6121 ctctgtgtga tggattctgc agagtgctgc aaatgtgtac tctgaatgat caacttcctt
     6181 gcgctttcgt cttcccagcg ctttgtcaaa gtgatcgata tcagctcctg ttttgccaca
     6241 gatgcagcag actctttttg taatgcattt gtagaagtaa tactcttgat tcgctggtaa
     6301 aatctcatat ccttctttga aaggaatatg atgttcaaag atgaaatcta aaatgatatt
     6361 tgctaagaca tt
//
LOCUS       DANKMN010000107         6230 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000107 DANKMN010000000
VERSION     DANKMN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            133..633
                     /locus_tag="PW224_002686"
     CDS             133..633
                     /locus_tag="PW224_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2909314.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="PW224_002687"
     CDS             730..2577
                     /locus_tag="PW224_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL2909315.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="PW224_002688"
     CDS             2580..3476
                     /locus_tag="PW224_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2909316.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="PW224_002689"
     CDS             3526..3915
                     /locus_tag="PW224_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2909317.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="PW224_002690"
     CDS             3902..>6230
                     /locus_tag="PW224_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2909318.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          925 c         1336 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag caaagcaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DANKMN010000108         6224 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000108 DANKMN010000000
VERSION     DANKMN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(207..1169)
                     /locus_tag="PW224_002691"
     CDS             complement(207..1169)
                     /locus_tag="PW224_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2909319.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="PW224_002692"
     CDS             complement(1171..2610)
                     /locus_tag="PW224_002692"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL2909320.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="PW224_002693"
     CDS             2795..4741
                     /locus_tag="PW224_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL2909321.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="PW224_002694"
     CDS             5005..5877
                     /locus_tag="PW224_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2909322.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1986 a         1061 c         1107 g         2070 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 atgaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 aggttctgtt gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata
     6181 gcaggaggaa tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DANKMN010000109         6175 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000109 DANKMN010000000
VERSION     DANKMN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            725..811
                     /locus_tag="PW224_002695"
     CDS             725..811
                     /locus_tag="PW224_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2909323.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="PW224_002696"
     CDS             complement(932..1621)
                     /locus_tag="PW224_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909324.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="PW224_002697"
     CDS             complement(1628..1906)
                     /locus_tag="PW224_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909325.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="PW224_002698"
     CDS             complement(1866..2780)
                     /locus_tag="PW224_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2909326.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="PW224_002699"
     CDS             complement(2762..3142)
                     /locus_tag="PW224_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL2909327.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3594..3881)
                     /locus_tag="PW224_002700"
     CDS             complement(3594..3881)
                     /locus_tag="PW224_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2909328.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3901..4125)
                     /locus_tag="PW224_002701"
     CDS             complement(3901..4125)
                     /locus_tag="PW224_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL2909329.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4580..4750)
                     /locus_tag="PW224_002702"
     CDS             complement(4580..4750)
                     /locus_tag="PW224_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909330.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4765..5298)
                     /locus_tag="PW224_002703"
     CDS             complement(4765..5298)
                     /locus_tag="PW224_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2909331.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5319..6161)
                     /locus_tag="PW224_002704"
     CDS             complement(5319..6161)
                     /locus_tag="PW224_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2909332.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1635 a         1152 c         1040 g         2348 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccaac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag cttactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccccactct ccacaataca
     3361 aaaaagacta gcaaatgcta gccacttttt gtaaagtttc gcttgtttca ccttcggtga
     3421 aattgttgca aatcaataat taggtagagc tacatttttt tcgttgttca aagattctta
     3481 tgccatttac tattaataaa tctataaata tgataatttt tcggctctat aatttatggg
     3541 actgatgaat cagaattgat tcttcagtcc cattttcatt ttattccaaa gaattaactc
     3601 caaataccaa tagaagccca tcctctatat acttgagatt ccttactaac aatttcttgt
     3661 aattctatta aagatttagg taatccaaat ttatgtttta aaatatatct tccaagaaaa
     3721 ctacttaatt tcatactaac ttcaccaaca ctttttccct cactgatttc tttagaataa
     3781 agaaagagct tttcttttaa gtcaggatag tcatctatag ttgcttcatt gtatacttta
     3841 ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca ttataataac ctccctacca
     3901 ctaccttcta ggtgcccatg gaccatgatt aacccaacca ttaacaataa tttttccaat
     3961 ttctccttta gcttgattcc aatctaccca acatttttct ttgttacaat aaagaccatt
     4021 tccatagtaa gtagctgcat caacttttat tcctgtaccg atgccagcta gacaagttcc
     4081 taatattcct agaataacac aatgttttaa tactttcttt ttcatgattg tttttccttt
     4141 tcgttttttt aaaattgctt gttatattaa ataatcttag taattttatt atataacatt
     4201 ttattatata acaatgatta acgtcataaa aagtagtttt gaattcataa tcgtttaaat
     4261 aaaattataa gatcagttat ttaaacagct agtagacata atgtatatac taccaagtaa
     4321 cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt ttattggtct aggttaaaag
     4381 ctctttttaa ggcttggttt tctgcggttg tttctttcta attctttgtg aatattgatc
     4441 ggtcattttt taaatacttg ttatttttgt ttgtgtttgt tatgcttttt acatatctca
     4501 tattcattta ttattctttt ctaaggttaa taataggata atctctttca gaaagactgc
     4561 tcgtacgagc agtcttttct tatttatcaa gtttttctac aaatacttcc tcattttgac
     4621 tgttgtagta attaggattg agttttagtt cccctttttt atttcctttg ttggccactg
     4681 ataataacat tgggctaaag cttaatattc ctagtaggat agaaccgaat agcaagtgtt
     4741 tttttgtcat aagtatttcc ttcttcattt ccatagctgc caccattttt tccaattctt
     4801 tgtttgttcg tccattgttt ctgttgcttc agaaagagtc gttgaactat tctctgaaga
     4861 ctcttccgta taagtcaact gcagttgctc ctgaagtttt tcgatttgtt ggttggcttg
     4921 cagggtgagt tgttgttgct ggtcgagtag tttttgtata tgagaattat tttgctcttt
     4981 gagggcaagt tgttctcgta aaaattcatt ttcttcacgc aactgatcta gcaacttgtt
     5041 tgcaaccacg tttttgtttg gtatataagc atttttatcg ttgttttgtt catgtttatg
     5101 caacttatct agcaaccttt gattgtctaa gttgcgagca agttgcaata ctccagaacg
     5161 caatacaacc ttataaccac gctcattttt agctatcatg gaagaatcta atttttggat
     5221 atgtttcgat attgcttgcc tactcatctt aaaaagatct gacaattcct ttattgtaaa
     5281 ggtttcttgt ggttgcatac tggttgcctc ctaggttgct actggaataa attatctaaa
     5341 tcagataata aacttttttt ctctttttcc gaaagtgcgg actctgtttg tttttcaaga
     5401 gcttttttct ctttttctag ttgtttgatt tgttggagga gttcaagttc tcgtttcttc
     5461 tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt attctttttg tttatcagct
     5521 aaagccaatg ctttattttg gtcttctttg gttggtgcat gtttgttaaa gtcgtctgct
     5581 tttttgatct caggtttgaa cgtaaattga tagccaataa caggttttcc ccgtcctttt
     5641 ccgtatttct ttttgattgt tagtcctttg aagattgcag ttagttcttg acgtataggt
     5701 tttataactc gtttatctat atcaggttgc ttataacttt taggaatatc taataattca
     5761 agaaagtctt ctttggagaa atatgcgaaa cctttggttc tgtattgctt taacagacga
     5821 aacatagtct ttgcatagcc actttgtagc tcgttgaatt gttgtaaaga atagcgtacc
     5881 cactcgtcta aattatttaa caaaggcagt gctttttcgt aaattcgtat atctacgtaa
     5941 ggattttcta cgtccccgtt gattttaaat tctgtaaaca ttacaaacat atcgtaagaa
     6001 agcccagact cacttctacg accaaagcgt aaagataaga ttttttcata agttgctttt
     6061 aaatcttcta caaatcgtac atttgcagtt gctttgtagt tacttaaatc ttttagttgg
     6121 tcaaacgtaa aacgaactgt cttatcccct ttttcacgca ttctagacac gatgg
//
LOCUS       DANKMN010000110         6050 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000110 DANKMN010000000
VERSION     DANKMN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..1062
                     /locus_tag="PW224_002705"
     CDS             <1..1062
                     /locus_tag="PW224_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDL2909333.1"
                     /translation="YYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLL
                     ILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATV
                     VGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDN
                     ETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNC
                     ASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYV
                     VRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINISYIKQ
                     LCYRGRNIISMIKSREHKR"
     gene            1098..1814
                     /locus_tag="PW224_002706"
     CDS             1098..1814
                     /locus_tag="PW224_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2909334.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            1833..3416
                     /locus_tag="PW224_002707"
     CDS             1833..3416
                     /locus_tag="PW224_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HDL2909335.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            3551..4129
                     /locus_tag="PW224_002708"
     CDS             3551..4129
                     /locus_tag="PW224_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909336.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            4075..4593
                     /locus_tag="PW224_002709"
     CDS             4075..4593
                     /locus_tag="PW224_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909337.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            4601..5641
                     /locus_tag="PW224_002710"
     CDS             4601..5641
                     /locus_tag="PW224_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2909338.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            5890..>6050
                     /locus_tag="PW224_002711"
     CDS             5890..>6050
                     /locus_tag="PW224_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2909339.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2191 a          695 c         1173 g         1991 t
ORIGIN      
        1 tattatatat cctatgtatg gaatcaattt ctaatacaat ttgctaaagg gttagaaaaa
       61 gttaaagcta tgggcatagc aggtattatc agcacaattg taacaataat tgccaatatt
      121 ttcttcttgc ttattctgaa atggggatta attggttttt tcattgcaaa tattttatca
      181 cagattattt cagctactta tctgtcagta ttagtgagaa tatgggaata tatcgatttt
      241 gaggctaaag atcctcagct aaagagagaa atgctaaatt actcgattcc attaattgca
      301 actgttgttg gatggtggat taatagtaca gctgataggt atgtggtttc ctttatagta
      361 ggtgtctcgg cgaatggctt attgtctgtg gcatataaaa tacctcaaat aattaatact
      421 ttgcagggaa tatttataca ggcctggcaa atttctgcaa ttaaagaata tggagataat
      481 gagactgcaa aattttatgg aaatacattt gttatgatta atttgttaat gtgcataacc
      541 tgttcaagtc ttatcatatt aactagaccg ttttcacata ttctttatgc gaaagacttt
      601 tatgaagcgt ggaaatatgt tccattttta ttaatagcta atgttttaaa ttgtgcatca
      661 ggtctgttag gacctatctt atcagcaaaa aaagattcta aatccatgat gtggtctgca
      721 atcatagggg cattagttaa tgtgattatg agtgtagtcc ttgtttataa gataggggtt
      781 caaggtgcca caatagccac tgttatatgt tcatatgtaa tatatgttgt tcgtaaacga
      841 gctgtgggaa aagatatttt tataaagcat tatcatattg ttgtggaatc ttggggatta
      901 ttgtttgttc aggcaatcat agaaatatat tttaaaaatt atgtaataga aattataatt
      961 atgattattt taattattat aaatatcagt tatattaagc agttgtgtta tagaggaaga
     1021 aacattattt caatgattaa gagtcgagag cacaaacggt agacgatgtt atttttataa
     1081 aaagatataa tttattaatg gtcgataatt atgaaaggaa aaatattctt aaaaaaaaga
     1141 gtataggatg tcgtatgaaa atagtcgcta tagtaccaat gaagttaaat aacaaaagat
     1201 tgccacaaaa aaacacgaaa gcttttacca atggaaaacc attgtgttat tatatacttt
     1261 caacactttt aaaagtaaaa cagattgata aagtgtatgt ctattgtagt aacccagata
     1321 ttaaagaatt tataccagaa ggtgtaacat ttttaaagag atctagttcc ttagatcagg
     1381 atacaacgag tattacacag gtattagccg gtttcacaaa agaagtagag gctgatgtat
     1441 atgtaatgac acacacaaca gcaccattta ttagcgaaaa atctattgaa aaaggactag
     1501 atgccgtgct gacaggagaa tatgattctg catttgctgc aaaaaaattg caagatttct
     1561 tatggaaaga cggtgaacca tttaattatg aattggacaa tattcctaga acacaggatt
     1621 tgccgccttt atacgaagaa actagtggat tttatattta taatcgcaat gtgatgaccg
     1681 agttgcatag aagaattggg aaaaaacctt gcatagttga agtaggtgag attgagtcag
     1741 ttgatattga tgaagccgag gattttatga ttgctgatgc gatttataat catcttattt
     1801 tacaaggtag ttaagacagg gggaaggtta ggatgaacaa tgtacagatt ttagattgca
     1861 cacttagaga tgggggctat tgtaacaatt gggaatttgg atttgataat acaaaaaaaa
     1921 ttatttctgg acttatagaa tcaggaattg agattataga atgtgggttt ctcacaaata
     1981 aaacagtata caataaaaat gttacaaaat atacgacctt agaagaatta gcacagataa
     2041 ttcctgaaaa aagagaagga aaattatttg tagcaatgat gaactatggc gaatataata
     2101 tagaggatct tccagattat gatggttcat ctattgatgg aattaggatt gcgtttcata
     2161 aaaaaaattg caatgaagcg ttgaaattat gtcgtgagat aaagaaaaaa ggctacttag
     2221 tttttgttca agctatggta tctctcaatt atacagatga agagttttta gcagtaataa
     2281 aaaatgtgaa tcagtttaaa ccatacgctt tttatattgt tgatagtttt ggaatgatga
     2341 aaggaaaaga tttaactcgt cttttttaca tgattgagca taatctagaa aaagatatac
     2401 agataggttt tcactcacat aataacatgc agcttgcata ttctaatgca caaaaactta
     2461 taaccgtaca aacaaatcga agcctgatta ttgattcttc ggtgtacgga atgggaagag
     2521 gagcaggtaa tcttaacaca gaactatttg tagaatattt gaatgataat gcaggaaaaa
     2581 aatatcaatt aaaaccatta ttatatataa ttgatgaaat actaaatagt ttctatcaga
     2641 agagttattg gggatattca ttaccaaact atatttcagc agttcataat gctcatccta
     2701 attatgcaag ctatctgtca tataagaata ctttaacaat tgaagcaatg aatgaaatat
     2761 tcgaaatgat ggatgaagaa aaaaaggtat cttatgataa acaatatata gaagatttat
     2821 actacaaata tatggaaact gggaaaatac aggaggaata tatggaagaa ctgagaaaat
     2881 ttctctcaga caaaaaaatt ctactgattg caccaggaaa aagttctgta gatgaggtag
     2941 aaagaataaa agagtttgtt accaaagaag aagtcgtaat aattagtgta aattttgaat
     3001 attctcatat agatatagat tacgtatttt taagtaattt aagacgtttt catgaattac
     3061 cgaaagaaag cagaactaaa tgtattgtta catcaaatat acaagcatcg gatgtatatt
     3121 tacggacgag ttataaaaag ttgttatctg aaagggaagt tgtaagagac aatgcaggat
     3181 taatggcaat tagattttta gccgatatga atgtggatga aatattctta gctggatttg
     3241 atggatattc acataatgaa gatgagaatt atggtgagca atccatggaa attataacaa
     3301 aatttgcatt attagatgca atgaacgtgc aaatgagaga tgaattgtca gaattagcta
     3361 aaaaagtaaa ggtgacattt ttaacggatc cacgttgggt tcgaattgat aagtagtagt
     3421 aataatgttt ttaaaatttt gataaagaga tagtgagaga ataaaaatgg gtaaagtctt
     3481 aaataaaaaa gaagaaaaat atttaagggc cgatggggcg agatgaaact ctaattttga
     3541 acttttaaga atcatttcta tattgtgtat tgttgcttac catgctaatt tgcgtttaaa
     3601 ttataatgca gacggaatta tagtacatgg ttcattaact gctaaccaga ttttcttgaa
     3661 ttatattggt atttggggtc agctaggagt taattgtttt atatttattt cggcatattt
     3721 tttaagtgag aaaagaaaca ttcactttga aaatgttttg ggaattgtat tagaatgtat
     3781 tttttatagt gtattagttt atggaattct ttgtgcatgt ggaatagaaa cattttcatt
     3841 gaacagcttt gtaaaagaaa tattatcatt tgctctaggt agatattggt ttattacatc
     3901 ctatttagca ttgtatattc taagtccgta tttaaatatc atgcttaaca atctggaaaa
     3961 acttcagttg caaaaaataa gtatgttatt gtttataggt gtatgctgtt atcgtatgtt
     4021 atttaaaaca tctttgtttg gggattgtgc tgttttaatt gcactatatg tcatatgtta
     4081 tacactgaaa aaaacgataa taatattttt gagagacaca gaatcatagg aacaattgta
     4141 tgtataacag ttataccact tctcatatcc ataggaatac ttttcaaatt tataccaaat
     4201 gcaatggtta accagatggt tcatattggt tctccattga tggttatagc aagtatattt
     4261 ttattttatg tatttaaaaa tttttcattt tacagtaagt atataaataa acttgcgaga
     4321 accagtttag gggtatatat tttacatgaa aatcattatt tcattgatgg tatgaagtct
     4381 agaggcttaa tatgggatgg gatatttgga ttaggcaaaa ggtttttaga tagggatttt
     4441 tggatttggt ttataattgc agttgtagtt gtgtgcataa tttgcatggc tatcgatgtt
     4501 gtaagagaaa aatttgaaag acagattttt aaaattaaga gtgtaaagaa cctgtgtaaa
     4561 cttatgaatc atgttataaa tatataccca taattaataa atgaggagaa aagaagtgaa
     4621 agttatatca ttcggagaat tattattgag acttgcagca cccggttatt caaagttatt
     4681 tcaaagagat agtttggaga ctacattctg cggaggggaa gctaatgtag cggtatcctt
     4741 agcaatattt ggatatcaat cagaatttat aacaaagctt ccggattcag atgttggata
     4801 tgcggcagct aatgctttgc gttattttgg agtaaatgtt gataatatta tatttggaga
     4861 gggacgcatg ggactgtatt atttagaaaa aggagcagct cagagaccat ctaaagtaat
     4921 ttatgacaga aaattttctg caatttcgtt agctgatagt gatgaattta agtggaagga
     4981 gatcctaaac gatgcaaatt ggtttcattg gacagggata aatccagcat tgtctgagaa
     5041 tctctttgag acgtccttag atgcatgtca ggctgccaaa aaaagaggta taacaatttc
     5101 ttgtgattta aattatcgat ccaagctttg gacatcagag gaagcaaaag aaaagatgga
     5161 acagatacta ccattggttg atatttgtat ttgtaatgaa gaagatgcag aaaaagtgct
     5221 aggaattaaa gctgagcata caaatattaa tagtggagaa ttaaataaag atggctatgt
     5281 gcaggtggct agggagattc acaagaagtt tggatgtagg tatgtagcta caactctgcg
     5341 tagaagttat tcggcaagta gaaatggttg gagtgcaatg ttatacgaag cagaaaaaga
     5401 taaagcgtat ttttccacag aatacaacat acaaattgtt gatcgcgttg gtggtggaga
     5461 cagcttttct gctggtataa tatatgcgtt atgtgaaaat atgtccggac aaaaggctat
     5521 tgaatttgcg actgcatcta gttgtttaaa acatactata gaaggtgatt ttaatcgaac
     5581 tacaattgag gatgtatata atttaatgaa tagcggtgga aatggtcgcg tgttgcgtta
     5641 gtcaacatat aaagataaat cacagtaatt tttatggtga ttatctgtct tagtagtgat
     5701 tagaataacg tgaaaataaa ttgttgagcg tttatactgt tttgaaaaat aaatttaaca
     5761 tattgaaaaa tacaaatata tacgtatatt ataaagcgcc gattgtaaaa ttaagctaga
     5821 caactaaaac agtcatttgt gctacactca aaatagttcc gaccaaagaa ttataaggag
     5881 tgagtgcaaa taacctatac ctatcttaca acggatgaac ttgtaataat agagtcttat
     5941 ttgaaaatta accaatctgt tgctaaaact gcgcattgct tgaatcgttc aagtcaaacg
     6001 atccataaag tatatctgtt cttcaagcaa agaaaatcag ccttagaata
//
LOCUS       DANKMN010000111         6020 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000111 DANKMN010000000
VERSION     DANKMN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(60..362)
                     /locus_tag="PW224_002712"
     CDS             complement(60..362)
                     /locus_tag="PW224_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909340.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(582..948)
                     /gene="ssrA"
                     /locus_tag="PW224_002713"
     tmRNA           complement(582..948)
                     /gene="ssrA"
                     /locus_tag="PW224_002713"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1119..1892)
                     /locus_tag="PW224_002714"
     CDS             complement(1119..1892)
                     /locus_tag="PW224_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2909341.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2036..2386
                     /locus_tag="PW224_002715"
     CDS             2036..2386
                     /locus_tag="PW224_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2909342.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2367..3083
                     /locus_tag="PW224_002716"
     CDS             2367..3083
                     /locus_tag="PW224_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2909343.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3083..4531
                     /locus_tag="PW224_002717"
     CDS             3083..4531
                     /locus_tag="PW224_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2909344.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4602..5111)
                     /locus_tag="PW224_002718"
     CDS             complement(4602..5111)
                     /locus_tag="PW224_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909345.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
BASE COUNT         2045 a         1050 c         1170 g         1755 t
ORIGIN      
        1 cccttttata agtatggtga taaaatccgt tccgggctac cttttttagg tagcctactt
       61 taatctttat acctttctgg atcaacgaaa gtatacttca aatagtcata acgccgatga
      121 tcgctccgtg cgtctggcac atcagtcacg atatcaaaca aaaaatacac atctttcttc
      181 attctggttt ttgttgccgg aattttaaag tagttcttat tagagtaata gagattgatt
      241 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
      301 ttgacaaatc tattcgaagg atcaaattta aaccataatt gtgtttttcc ttccatcaac
      361 ataatgttca cctcaaaaag agtatacgaa ctaatgttct ttttgtaaag taggaatatc
      421 attgataata tggatggaac atatatatta aaaagtgttc tttttgaatg ctattttgaa
      481 tgccattttc attcatttaa cgaaaatctg cgaaaataaa aaatgctcaa acccttataa
      541 aataaggatt taagcatcta gcgaaatctg ctgaaatctc acaatggaga cggcgggagt
      601 cgaacccgcg tccaaacaca tcgccactta aaaatctacg ttcatagtca actcatttaa
      661 ggattcactt tacagcttgc cgagtgacag gcattctgta tcgctagtct gatgttctct
      721 ttttcaactt acagacggaa agttgaaacg tatcccacta aagttaggac ccttactcga
      781 gcacatgggc gatgccgaga ggatctacgc taactgtttt taggcagcta aagcgtaaga
      841 gttgttttcg tttttagcag ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac
      901 gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa
      961 gggttacgtg tatttttcat acaaaattag tatagcatat attgggataa attcaataaa
     1021 aaagcgaatc atatcccatt atttatataa taactgcccc tttcttaaga aaaaataagc
     1081 gattttctta agaaaagagg tagcttcaaa aaaagatctt aaatcagatc aaaatacttc
     1141 aatgcgtgga caattccgcc ttctgtattt ttctttgtga taaaggtaga aagttctttt
     1201 aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa
     1261 tcattcggac catcgccaaa tccaaaagta ggaacttttt ccagctgaag ttctttttgc
     1321 agacgtttga cacctgcccc ttttgagatt cctttcttca ctgtatcgat cgagtaagga
     1381 ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc acctgattga
     1441 gataagatca gcaacatatt tactgtgttt tctttaaagc ctaaaggatc aatttccgga
     1501 acatctgaat gaatgaagtc gtaagcattg atcattgttt ggctatgacc ggtacaccag
     1561 attttctgat cgttatagta agaaagttca tgaccgttca actgtacatg atcatacatt
     1621 ttttgacatt cttctgaaga aaatgtttga gaatagattt cttcgccgtc tactcgaatg
     1681 aaagaaccat tcatggtgac cgcagaagta atgcccgcat cttccatgat agagtggatt
     1741 tctgcttctg tacgtcctgt agcgatgacg gggagaacat tgttttcttt taatgcgttc
     1801 atagcttttg ccacttcggg tgtgattttt gatttcgcat ctagtaatgt tccatcaaga
     1861 tcaaagaaag tgattgcacg ataatttgac acaaataaaa ctcctttttt attggaataa
     1921 gtttatctta acaaagaatg aacaaaagac aaacctttcg tgaagaattc ctgaacatat
     1981 tggttcagtc aacaatttag agtatactat aagaagagaa tttaggaggg aaagaatgat
     2041 ttttttcagt attggtatct tattcttaat aattggcagt ctttttcgga ttctgccctc
     2101 aaaaggaaat ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa cagacgctca
     2161 ttggcggctg gctcaaaaga caagcggaaa ctggtttttt cttatgggct tactgatggc
     2221 attgatcggc tattatttaa agacatctgg gcatacgaat tattttctga ttgaaatgct
     2281 tctgctagta tttccaatta tgcctatttt tattatgaca gaaaagaaat tacagaaata
     2341 tgacctggaa actggaggga acgataatga atattttaat gattgaagat aatcaatctg
     2401 tttctgaaat gatgcagatg tttttcttaa atgaaggttg ggaagcaact ttcaagtatg
     2461 atggaaaaga agggctcgat gcctttttag aaaacccaca acattgggat atgattacac
     2521 tagacctgaa tttgcccaca ctggacggta tgctagtagg aagagagatc cgcaaagttt
     2581 caagtactgt gccgattatc atgctgacag ctagagattc tgaaagcgat caggtgatcg
     2641 gcttagagat gggggcagat gattatgtca caaaaccttt cagtcctctg acgcttattg
     2701 ctcgaatgaa agctttgcat cgccgtagtg agttggcaga agcaagagaa gaagcagtag
     2761 tttcagatga tcattttgat atcgagacta gtcatttcaa gatgaatacg aaaacacgag
     2821 aagcgtatct ggacaaccgg ccaattgaag gactgacgcc taaagagttc gatctattgt
     2881 ttactttagc aaaaaaacca cgtcaagtat tctctcggga gcagttgctt gaattagtct
     2941 gggattatca gtactttggt gatgaacgga cagtggacgc ccatatcaag aaattacgac
     3001 aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt ttggggagtc ggctacaaat
     3061 tcgatgattc tggtgttgcc taatgcgtta tttgtatcag caactcttag ctttctgcgg
     3121 aatggtcgcg ttgatcttat tgatcgttgg agtttctttt acacaactga caaaacaaac
     3181 gattgaagaa aacaattacc agcaactgtt tggatatgct gaatcagttg aaaagacaac
     3241 gcaaacctat gccgatacgc tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt
     3301 gctgctgaca gagcaagtac tgacagaaca aaatgtgaac tttatattta tcgataaaaa
     3361 cgaacgtgtc atttatccga ctaatacagc tttactgaat tttaccttaa cagctgatca
     3421 atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa atattttagg
     3481 acaagcacag gcgacctctt acgcacttgt gccatttaat ttgaatcatg aattctatgg
     3541 agcacttgta gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc caattacact
     3601 gaacctgttc aaaggattta ttttttccag tatcgttgca gttattgcca gctatgcttt
     3661 tgcagcattc caagttaagc gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa
     3721 tggtaatttt gatgtacagc tccccgtaca tgacaaagat gaatttgatg agttagcaga
     3781 tgatttcaat aaaatgacga attcattgaa agaatctcaa gcagaaattg aagaacaaga
     3841 aaatcgtcga agacaattta tggcagatgc ttctcacgag atgagaacac ctcttacgac
     3901 aatcaacgga cttctggaag ggttagaata caatgcaatc cctgaaaatc agcgagaaaa
     3961 cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga atgaaaactt
     4021 ggattatgaa aaaatccgta cgaatcaaat cagtatggtc attaaaaaat tcaatggaac
     4081 agaaacattg aaaaatattg ttgcacagtt agaagctaaa gcagaagcag caggagatac
     4141 attgacattg aaagccgttg atgatattga tgtttacgct gattatgacc gatttgtcca
     4201 aatcatggtc aatattattc aaaacgcgat acagtttact gaaaacggcc aaatcacgat
     4261 tactttagaa aaaggctatc ttgaaacgat catcacgatt gaagatacgg gaattggaat
     4321 gtctgaacaa caaatgaaaa gtatctggga tcgttattat aaagtagatc cttcaagaaa
     4381 aaatacaaaa tatggtgaat caggattagg tttaccaatc gtgcagcaac ttgtacgctt
     4441 gcataaagga aaattagaag tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg
     4501 tttgcctgac gtagaaatca aaaaagagta aaaaacaagc ggtataaaag atcatgaatc
     4561 gatcttctat accgcttgtt ttttactata aaatgattga tttagcccgc gggctctaaa
     4621 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4681 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4741 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4801 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4861 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4921 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4981 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     5041 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     5101 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa agtgaaaggg
     5161 aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc aaatccctta
     5221 agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt ctgttgatcc
     5281 atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg cagttcagta
     5341 gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga ctggaagctt
     5401 acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag ctgccagaaa
     5461 ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat caattcctgc
     5521 ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa tagaaaaaat
     5581 aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg aagaagctct
     5641 gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc taaagccgat
     5701 tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct tgaaaataaa
     5761 caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata gcggattatt
     5821 ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag tatgaaattc
     5881 ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag aggttgtgaa
     5941 gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta ttcgaaaaga
     6001 ggagcagcga caataatttt
//
LOCUS       DANKMN010000112         5954 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000112 DANKMN010000000
VERSION     DANKMN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..813
                     /locus_tag="PW224_002719"
     CDS             <1..813
                     /locus_tag="PW224_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL2909346.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="PW224_002720"
     CDS             807..1229
                     /locus_tag="PW224_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909347.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="PW224_002721"
     CDS             2399..3367
                     /locus_tag="PW224_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2909348.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="PW224_002722"
     CDS             3384..3908
                     /locus_tag="PW224_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2909349.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="PW224_002723"
     CDS             4284..4565
                     /locus_tag="PW224_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909350.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="PW224_002724"
     CDS             4567..4983
                     /locus_tag="PW224_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909351.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="PW224_002725"
     CDS             5613..>5954
                     /locus_tag="PW224_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL2909352.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2258 a         1018 c          965 g         1713 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggtctagtcg acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DANKMN010000113         5769 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000113 DANKMN010000000
VERSION     DANKMN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(1014..1304)
                     /locus_tag="PW224_002726"
     CDS             complement(1014..1304)
                     /locus_tag="PW224_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909353.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="PW224_002727"
     CDS             1706..1942
                     /locus_tag="PW224_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909354.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="PW224_002728"
     CDS             1949..3901
                     /locus_tag="PW224_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2909355.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="PW224_002729"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="PW224_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2909356.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="PW224_002730"
     CDS             complement(4797..5489)
                     /locus_tag="PW224_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2909357.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          996 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DANKMN010000114         5335 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000114 DANKMN010000000
VERSION     DANKMN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(15..410)
                     /locus_tag="PW224_002731"
     CDS             complement(15..410)
                     /locus_tag="PW224_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909358.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(400..612)
                     /locus_tag="PW224_002732"
     CDS             complement(400..612)
                     /locus_tag="PW224_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2909359.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1684..2286
                     /locus_tag="PW224_002733"
     CDS             1684..2286
                     /locus_tag="PW224_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909360.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2641..2877
                     /locus_tag="PW224_002734"
     CDS             2641..2877
                     /locus_tag="PW224_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL2909361.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2874..3287
                     /locus_tag="PW224_002735"
     CDS             2874..3287
                     /locus_tag="PW224_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2909362.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3806..4756
                     /locus_tag="PW224_002736"
     CDS             3806..4756
                     /locus_tag="PW224_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDL2909363.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4753..>5335)
                     /locus_tag="PW224_002737"
     CDS             complement(4753..>5335)
                     /locus_tag="PW224_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2909364.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1908 a          760 c          901 g         1766 t
ORIGIN      
        1 atgaaccttt atttctacat ttttttctca agcacactac tgataatgat taaaataatc
       61 ggctggattg caatgatact tccgacaata atagctggaa tgatgtagtc taaacaaata
      121 aatataactg ttgccacccc taacagtatt gtgttaaaca tatttgttat atttcccgct
      181 ttttctttga ttatgatatt ccgttcgtcc ataacattga ttttgtagtc tgcatcattt
      241 ttaatcattt tatcatgcaa aatttgaaca tgagaaacgc caaaaacggc actgaataaa
      301 atagcaagtc caatatctac cattttagga aaatctgtaa ccaaaattgt cacaatcaaa
      361 aacaacgaaa caatataacc gatataccaa atctgaccct tatttttcat cttcactttc
      421 ctcctcatag ataaaaattt cttcaatgct catttgaaag taccttgcaa ttttaaatgc
      481 caatataata gagggattat aacgtccatt ttctaaagaa cctattgttt gacgagatac
      541 ttcaagagca gaagctaaat cttcttgttt aattcctctt tgttttcgta attcttccaa
      601 acgatttttc acaacatgct cctttctaaa aaatggaaag tttactttcc tttcgtgctt
      661 ttataatact cctgcgagca caaaatgtca aggaagcttt ccatataaat tttgatttta
      721 atgttttagt tactgttttt atgacacatg aacacactta ttcttatttc ctcttatctg
      781 ctatcaacag aaaagaaaaa gccctgtcaa gagccgatta gatcaagcca gtcgagaaac
      841 gatgttagac caaattgatt ttgggtaatg aatgtttaga acttagtagt catccaaaaa
      901 cgactacttt ttttgttgaa tcgtccaagg acgattcgat gcttttgact tgtaagaaca
      961 ggattgaatg aaaccatact gttcgcatat ttttagtgga tgaacaaaca aaatacgaga
     1021 gattttttgt tcgttcatcc atggttttag aaaaaagagg gacgattttg gaagaagaaa
     1081 atcgtctctt ttttttcttc tttttgtatg acaaaaagaa agatcttttg cccattttat
     1141 ttttataaaa tgggtaggtg gcgttcgcgt aaagcaaatc gatacaatcc aatggttttg
     1201 gcgaatcaga tttaccattc taggcttgtt taatacagac atcgattgtt attttgagaa
     1261 tttatatcct tattggcaat gaaatggata ttctccatac caatagaaat cttcaagata
     1321 attttcggaa atatataaga gttaatcaat tatgatcata tagtaggtaa aaattaggac
     1381 agccttcaat tatatgggaa accaatataa tcaagtaatt attcgggtaa cacaaatact
     1441 agagtactca tttcttataa ctgttttaca cctattaggg atcagtatta ttaatagatc
     1501 tagataaatt aatacgtaat tatataataa tttatgtttg tgccattaaa caatcttaac
     1561 agatatttca ttttatttat gtaaaacaac acgaataatt attgcttcgt aaagttatat
     1621 gagtataagt taatttactg gaggtagtgc aatgcttttt tctgaaagat ttggttttaa
     1681 aaaatggagg atatcatact gagagataga ttgacagacc ctcttaaaaa tagtttagca
     1741 aatgtgattt atgatatatt acgagaggct gataaagttt atagtaatta taatggaaaa
     1801 gtctcatggg taatatattc ttttttaatt aaagagtttt ttaaagaaca gataaataat
     1861 agctatgagt tgggttatca ttttactgtt tattatgaag atattatcaa gaggttagaa
     1921 gacttggagt ggtatgctta ttatgattta ttagaggttt ttgcaagtgc taaaatcttg
     1981 gataaaaaga aaagagagga agtaaataaa attctggagt cggaaaattc tggttacaga
     2041 ttaagcgaaa gcaatgaatt tattccaatc acagatgata tttctattaa gtctataaat
     2101 gctgtatctg aatcaccatt taattacgca aaaaatcata tccataaatc tcttacatat
     2161 ataagcgaga aggaaaatca agattataat actgtagtga gagaagcaat tagtgctgtc
     2221 gaaagttgtc ttatcgaatt ttcaggatta ccagctaata aaaaaatact ttgggaactg
     2281 ctataaaagc aattaaagac aaatcgaata acaatgtcga tcttacattt ttaaaaccat
     2341 ttgagacaat gtatggtttg gcgagtaata atggaatacg acatgcagaa aatgaaaaaa
     2401 caattgtctc agatttacct gatgcagttt tggtgttaac aacatgttca gccgtaatta
     2461 attatttggg agtaaaaata gcatatgact ccaataaatc atgaaaagtt aaaattcatc
     2521 caagagctac attataacta ttaatttcca actctttaaa agtttagtaa ttgaaggaag
     2581 taataagggt gattgaacaa tacggatata cggtgtatac ttatcgtagg agttgatgat
     2641 atgaatatta aaaaacgcaa aatcagagaa gtaggtaatt caatagttgt aactttgtca
     2701 aaagagagtt tattgcaaaa aggtttgaaa cctggagata ctatttttat tgatgaagat
     2761 aaaatgatgg aagctattgt aaaagaggaa agcaagatag atcttgaaat tgatatgtat
     2821 gttaaccaag ctttatctga gtatgatgca gctttcaagg aattagttga aaaatgatct
     2881 atctaagtga aaaagatatt gtaaaattga atgttattca aataaagaaa tattcaccgc
     2941 gagaaacaat cggagtcatt gacaaaaatg cgctgagtat ggctgtaaat caacctaaac
     3001 agcatatttt tggaaaagat ctgtatcctg ataaatttag caaagcagct gttctgctaa
     3061 taaatctgat caaaaagcat ccatttcata atgctaacaa acgtacagca tttttagccg
     3121 cgtatgtttt tctcaagcta aatggctatt cactaaaaat gaaaaatcag gaagtagtag
     3181 aatttgtagt tgaaattgct acgtatcagg gtgattttga tgacttaaaa gaggcaacaa
     3241 cagatatact aaaaaagaat tcaacaaaga ccgattcaaa aaagtagaag caaactaggg
     3301 attcagttaa atctgaagat ttagtttgtt ctttatttta gtttacataa gatacattat
     3361 gcgaagttca ttattttaaa agcttaaata ataaactttt ttataatacc aatttattta
     3421 tgtaaaccaa tgaccttaat cgcttaagca agcatgttat gtaatactct actctctcca
     3481 aaactaatga ctatctattt tcaaagaagc tagaatgatt atctgatcaa atgataattt
     3541 atatttaatt cctggtctac tgctcttctt ttaatcaatt aatgtctaac gtactgtaca
     3601 cgataaagta ggaagactta ttttcaactt cggattcatt tttttaattc ggtgaaaatc
     3661 actaaattaa gagtttatcc aagttcattt tagctgagaa tacgcttgaa aataattttg
     3721 atgaattttc ctgaattaaa atttattaac ttgttgagtg atataattgt aatgtattac
     3781 caaatagtta gcacttagat tagtgatgga aaggaatagt gagaacaaga tgactggtag
     3841 aaatcccaaa gtatttatat catattcatg ggagaataag gaaaataaag aatgggctag
     3901 agaactagca gacaaattac ttgaaaacgg tattgatgca tatatagatc agtatgattt
     3961 aagtttaggt gatagattac ctcagtttat ggagcagcaa atatccgatt cagattatgt
     4021 tctaattata tgtacaccag agtataaaaa aaaggcaaat aatcgtacaa gtggtgttgg
     4081 gtatgaaggt catattatct cagcagaatt gctttctgga aaaaatgaac gtaaatttat
     4141 cccaataatt aaagaaggac gtgcaagcga agtacttcct acttttcttg ctggaaagct
     4201 tggaattgat ttatcagata gccagcatta tgatgaaaac ttagatgatt taattacaac
     4261 tatatttggt gttaagagaa aaccagcatt aggtgatata cctaggcata ttaaaggcag
     4321 tcctttacta cctatagaac ataatgaaaa taaagatatt gaaatcttag gaattatcac
     4381 tgataaagta actgtaccta aaatggatgg ctcaaggggt tctgcattat ataaaattcc
     4441 atttagatta aatagagtac caagtcagct ttggaaagag ttgtttgtta gaaattggaa
     4501 ttcaccacca cgatatacaa caatgcatag accaggaatt gcttctgttg tagacgatga
     4561 aattttactt aatggaacaa ctattgagga agtccgggaa tatcatcggg aaactttaaa
     4621 actttgcatt gaaaaaagta atactcaaga aaaaaaaata ttgctagagg aggaacaaaa
     4681 aaaagagaga gagaatgagc gagtcagatc acataaagaa agtgttcaga aattagcaga
     4741 tgaattagag ttctagttga tagtcagttc tttcttaatt cgatacacag tttgtctagt
     4801 tacattatag tctgttgcta tttttgaaat agcaacacca ttctcctggt cctgaacgat
     4861 gcttctataa acaaggcgac gttgaggatc ttttgtgttc gcactgtata acttaggacg
     4921 tcctttatag acgccgtttg ctttagcaat ctttatccct tgtgcctggc gtcgttttga
     4981 ttcagtacgc tcttgttcag caatcatggc caagatttga atgattaggt ctttgataaa
     5041 tttatccagg agaggatttc cgattgcttc agccatgatc ggtaaattag tgataataag
     5101 tcgcacattt ttcttcttta gataatttac tgaatcgata atctcgtcat aattacgtcc
     5161 aagtcgatcg atagcctcta caataaaaat atcttggtct ctaacaaaat ctagaacttc
     5221 ctgaaaaatc ggtcgatgag taatagtcgc tccagattgt ttctcgatga aaattttttc
     5281 agcaccaaat tttctcattt cctcgatttg acgttgttca ttttgatcga tcgat
//
LOCUS       DANKMN010000115         5311 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000115 DANKMN010000000
VERSION     DANKMN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PW224_002738"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PW224_002738"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL2909365.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="PW224_002739"
     CDS             complement(1297..2133)
                     /locus_tag="PW224_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2909366.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="PW224_002740"
     CDS             complement(2317..3534)
                     /locus_tag="PW224_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2909367.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="PW224_002741"
     CDS             complement(3683..3862)
                     /locus_tag="PW224_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909368.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="PW224_002742"
     tRNA            3917..4000
                     /locus_tag="PW224_002742"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="PW224_002743"
     CDS             complement(4240..4818)
                     /locus_tag="PW224_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909369.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4824..5207)
                     /locus_tag="PW224_002744"
                     /pseudo
     CDS             complement(4824..5207)
                     /locus_tag="PW224_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DANKMN010000116         5207 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000116 DANKMN010000000
VERSION     DANKMN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            34..1368
                     /locus_tag="PW224_002745"
     CDS             34..1368
                     /locus_tag="PW224_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909370.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1355..2989
                     /locus_tag="PW224_002746"
     CDS             1355..2989
                     /locus_tag="PW224_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909371.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3164..3997
                     /gene="msrA"
                     /locus_tag="PW224_002747"
     CDS             3164..3997
                     /gene="msrA"
                     /locus_tag="PW224_002747"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2909372.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4110..5066
                     /locus_tag="PW224_002748"
     CDS             4110..5066
                     /locus_tag="PW224_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2909373.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1825 a          806 c          951 g         1625 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttcaat caaaaaataa tttctcagcc gggacatatt gttgtaaaca aagaaacagt
     2581 agaggcgtat accactaaag gaatggatta ttttttatat gattattctc ctatcgcttc
     2641 attggagaat aaggagcata taataaaaca cgaagcaaag attggcagtc aatgggtaaa
     2701 ccttccttat aaaacaactg gtagttctat catatataga gtaactaatg agaagagcca
     2761 agtaatcaca cttcctgtat atgcttattt aggaacgaat gtagaaataa atcatcaacc
     2821 tgtaaaggag caggtgaatg ctaatggatt ggtacaaata agcggagtca aaggagttgc
     2881 tgatattaaa gtaagcacaa gttatacagt aacgactatt atttcttatc ttgttacatt
     2941 aactgcatat ttatgtttat tttttaaaat agttttccat aaaaggtaaa ttaacttttc
     3001 atttataaaa taaattgatt aaaaaggaga atctgtcgat aaggattctc tcttttttat
     3061 acgaaaaatc tgtaggggga tgaagtaatc gcttatttcc tcactttatg ataaattaca
     3121 gctaaagtca ctagcttgtc ttcttcggaa aggaggagaa tctatggatg aggaacaaat
     3181 attcattcaa acactttata atctgatttt gaatccgaat acacgagatt gggaacgaaa
     3241 agtattgatt cagacaaaga atgacataag agaaaatata tccgttaaag aacagttatc
     3301 aaaactagaa gcaacactac gtccattagc tatccgaatg aatctgacgc ctgatgtaat
     3361 ggacttttat ttattgatta cggaaggatt tgataaggag caaaaatatg acttttccaa
     3421 gcatgccatg caagatgctg actatcaaga acgtgcagta tttgcagggg gatgtttctg
     3481 gtgtatggtc gagccgtttg aatcgaaaaa agggatccta tctgttttgt caggatacac
     3541 gggcggacat gttgaaaaac caaattatga tcaagtaagt ggtggttaca caggtcatgt
     3601 ggaagctgtg gagatcattt atgacacaag agaaataagt tatagcgagt tgctgacaat
     3661 ctattggcaa attactgatc ccacagatac atttggacaa tttcaagata gagggaagca
     3721 atatcgtcca gtgatttttt atcaaggcga acgacaaaaa gaattagcgg aacaatcaaa
     3781 acagaaacta gacagttcag gaactttcca ccaaccaatc gtcacaaaaa tcgaaccagc
     3841 cggtacgttt tggccagcag aaaactatca tcagcaattt tacaaaaaac aaccgaaacg
     3901 ttataaaaaa atccagcaag cacgtaatca atttctgatc tatcaacgag taaaaaacaa
     3961 atggcaaaaa aatatacgaa aaaatcattt tgattaactc cttttcttta aaatgtagta
     4021 aactaaaaat agaaaaaaga aagcgtttta aaaagatttg atgaggagga gaaaaattgt
     4081 atattgcaga tagtgcacgt tatgaaaaaa tggaatatcg taaatccggt cattcaggtt
     4141 tgaaattacc gattttatct ctcggattat ggcaaaattt tggtgattat gatcctatcc
     4201 ataatcaaag agagatttta agaggcgcat tcgacatggg aattacccat ttcgaccttg
     4261 cgaataatta tggaggccct gctggagctg cagagaaaaa ctttgggcgt atttttagag
     4321 aagattttca agcctaccgt gatgaactga ttatttctag caaagcaggt tatcatatgt
     4381 gggaagggcc atatggggaa tggggctctc gtaaatccat catttcaagt tgtgaccaaa
     4441 gtctccaaag aatggggcta gattatgttg acatcttcta tcatcatcgc ccagaccctg
     4501 atacaccttt ggaagaaaca gcagaagctt tgatgcaact tgtaagacaa ggaaaagcat
     4561 tatatattgg tatatccaac tataatggag aagatacaaa aaaaatgact gaaattttaa
     4621 aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt tctcgtgcac
     4681 tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca atcacgttca
     4741 gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct gaagattcta
     4801 gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca actctggaaa
     4861 aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg caaatggccc
     4921 ttgcatggaa tttgcgacaa aaatctgtta caagtgtact ggtcggagca agtcggttaa
     4981 gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca gaagaagaat
     5041 tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat ccatatatta
     5101 aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg aacatcatat
     5161 tttttgtcgt tcaaaataaa aatgatcgaa ggctaggagt gaatttt
//
LOCUS       DANKMN010000117         5135 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000117 DANKMN010000000
VERSION     DANKMN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(383..1849)
                     /locus_tag="PW224_002749"
     CDS             complement(383..1849)
                     /locus_tag="PW224_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2909374.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2140..4224)
                     /gene="cadA"
                     /locus_tag="PW224_002750"
     CDS             complement(2140..4224)
                     /gene="cadA"
                     /locus_tag="PW224_002750"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2909375.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4749..5108)
                     /locus_tag="PW224_002751"
     CDS             complement(4749..5108)
                     /locus_tag="PW224_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL2909376.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
BASE COUNT         1549 a         1103 c          830 g         1653 t
ORIGIN      
        1 aatggtgttc aatcagctga ccttcagcat tagctcaaaa agcggcaaaa tatgaggagc
       61 tatttttccg tttgtttctc taatactcag gtcaaatcgc tgattttaca acctcttcct
      121 taaacggtgt tccaaagctt gcatctgcgg taataagccc agccaaacga aaaatatgag
      181 gggctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
      241 gattcacggt gttccagaag tagcttcttc gaaaaacaag ccgaaacttc acaaaaattt
      301 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
      361 tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca gtgatttctt
      421 tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc aagacgattg
      481 gtgtgatgat attcgtaaat aattggaacc accatgcttt aggatcagca gtataatcta
      541 cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac caagcaccaa
      601 agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat ttcttataag
      661 ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc atgacaactg
      721 agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca aaaatcggca
      781 acaaaataat gattcctact agtatacctg tcagaagata accattcacc aatgtacctt
      841 tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct gctaacaaga
      901 tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc atgtttgtca
      961 atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa atttgaaaag
     1021 cactgtatgc cccatttgcc atcaagtctt taggaatatt atcactagca aacagcatac
     1081 ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag aagatcatcc
     1141 ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat attttttccg
     1201 cccctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa tcaaatgttg
     1261 gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt ggattgatga
     1321 acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc gctgttccgg
     1381 ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat aaaaaggcaa
     1441 gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt acgatatttc
     1501 catttccttg gactgcccaa ccaccagcaa tcagaattgc ttgaggcttt tgtgccaaat
     1561 aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt ttattagatg
     1621 tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct gatcctaatt
     1681 gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc caagaggtga
     1741 caactgatac tccttgctga gcatagttat taactacatt ccccatgccc caaaccatgt
     1801 taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt cgttctctcc
     1861 tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca aacttacaag
     1921 acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt catatttttt
     1981 ccatcaaaaa aataagttat tttcttttat ttcattgacc ttattgaatt tcaagctatt
     2041 ttgaattttc tgcaaaatta aaaacattag ttattttata atcctatcta ttttctttac
     2101 atttttcatt aagaaaagga tataaaaaaa agaggacgtt cattcgtcct ctttttttac
     2161 atgcaagctt cgcatggcat tcaaaacagc taatactgtc accccaacat ctgcaaatac
     2221 tgcatctccc attgatgcaa aaccaaaagc acctaaggca agtaccaaaa tttttatccc
     2281 aatagcaaag acaatgtttt gtttgacgat tttcaatgtt ttctttgcta aatggattgc
     2341 ttctgcaatc ttcgatggtt gatcattcat gatgactaca tctgctgctt caattgcagc
     2401 atcgcttccc aatccaccca ttgcgattcc tacatctgct cttgctagca ccggtgcatc
     2461 gttgattcca tctccgacaa atgcggtctt tttattgttt ctttgtaaga tttcctctaa
     2521 ccgctgcact ttatcttcag gaagcaattc gctatatatc ttgtccacgc ctacttgctt
     2581 cccgatatga tcagcaattt ttttcgagtc gcccgtcaac atgactgtgt tcttcacacc
     2641 agcctgtttt aattgaacca atgcgtctac ggcatcttct ttcaaggtat ctgcaataac
     2701 aagataacca ctgtaacttt gatcgatcgc tagataaagc aatgtaccga tttcttgact
     2761 taaaggaaaa gagatttcaa atctttccat caatttgtga ttacctgcta atacttgttt
     2821 gccatcaact tcagctaaga tcccttcacc tgcaaattcc tggatattag ttgttggtaa
     2881 aagcggacct gcgtagcctt ctaaaacaga ttgagcaatc ggatgtgtag aaaactgttc
     2941 tgcactcgca atatattgca tgaatttctt tggatcgatt tttgtatcga tcgtctggat
     3001 cgaaaagtca cctttcgtta atgtgccggt tttatcgaat accactgtct cggtttgcgc
     3061 aagcaactcc aagtaatttg aaccttttac taatacaccg attttacttg cgcctccgat
     3121 accgccaaag aaactaagtg gcacggaaat cactaatgca catggacatg agatcactaa
     3181 aaatgtcaat gctcgataga tccattcaga aaacgccgct ccagaaacaa caagtggagg
     3241 taaaacagct aataaaacgg ccaatcctac aacgactggt gtgtaatagc gcgcgaattt
     3301 agtaatgaaa ttttcagctg gtgctttttt actgctggca ttttctacta attctaataa
     3361 tttgctgata gtagaatttt cataagattt ttctacttga actaaaaatg gtttattttt
     3421 attgataaat ccgcttaata ctgtttcacc aacatataca gatttaggga ctgattctcc
     3481 tgtaagtgct gacgtatcga ctaatgattc tccttcgatg atcgttccat ctaacggtac
     3541 acgttcacca ggcttcacaa gtacaaactg accgatctgc acgtcttcag ggttcacggt
     3601 catcaatcct tccgtcgtct gcacgtgagc tgtttctgga cggatcgcca tcaattcgcg
     3661 aatcgatttt cttgaatgac tgactgccaa tccttggaaa tactcgccga tttgataaaa
     3721 tagcatcact gctacggctt ctggatattc accgatcaac atagcgccaa tcgtagcaac
     3781 agacataaga aaattctcat caaagatttt tcctttgacg atattcatca ctgccttctt
     3841 cactacatca tatcctatca gcagataaat caataaatac cctatcaagc gaattggttc
     3901 attaaaatca aataatagca ataaagcgat acctgcaagg gaaaataaaa tccggaacag
     3961 cgtatttctc tctttttcat ttccatgttc agatactttt tccttatcta tttctgttac
     4021 tttgacatct ggttctaatt ccttcactac tttttttgtt tcttgttcaa tcgtagccaa
     4081 atgatcttcc tctgcgtcaa tcgttagttt tccagaagtg aagtttaccg ttgcttcctt
     4141 gactccattg atcttctgaa ctcctttttc gattttcatt gcacagttcg cacaatctaa
     4201 accttctaat ctgtaacttt tcattttaat gacctcctcg cctcaacata tgaacatctc
     4261 ttcataacta attataaatg aatggttatt catatgtcaa ggtatccgaa agaaaaaatg
     4321 aaaaacatct gcatcggaaa atttcttcca tacagatgtt ttggcttatt ctttcttttt
     4381 ctcttcgatc attgcccctg ctcttatcca acccgcactt gccttcttga tcttgacagg
     4441 atagcaacta agtttgaagc ctgttgctgg caatttgtct aaattcatta atttttcgat
     4501 aggacaatat cttttttcaa tccctgcaaa atgtccttcc cagatattcg aagctccact
     4561 acgctttcaa aaatatagaa agagttcttt aatgaaaatt atttcaactt tccataaaca
     4621 gtaatcccat ataaataaaa gacgtaaaag tcacatggga gaacttatac aggaaaacac
     4681 atagagtcag gtgaaataca tgtatcccac ctgactctat gcgttttcta acctttatta
     4741 ttctcttttt atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa
     4801 gattgcttgg caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca
     4861 tgtgcgagag caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac
     4921 atgccagact ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc
     4981 ttaaaacatt gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct
     5041 gctaaccagc taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa
     5101 caactcattg tatttctcct tctattactg ttttt
//
LOCUS       DANKMN010000118         4943 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000118 DANKMN010000000
VERSION     DANKMN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(50..1090)
                     /locus_tag="PW224_002752"
     CDS             complement(50..1090)
                     /locus_tag="PW224_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2909377.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            2256..2342
                     /locus_tag="PW224_002753"
                     /pseudo
     CDS             2256..2342
                     /locus_tag="PW224_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2902..3120
                     /locus_tag="PW224_002754"
                     /pseudo
     CDS             2902..3120
                     /locus_tag="PW224_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3380..3634
                     /locus_tag="PW224_002755"
     CDS             3380..3634
                     /locus_tag="PW224_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2909378.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            3635..3952
                     /locus_tag="PW224_002756"
     CDS             3635..3952
                     /locus_tag="PW224_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2909379.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            4511..>4943
                     /locus_tag="PW224_002757"
     CDS             4511..>4943
                     /locus_tag="PW224_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909380.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT         1727 a          707 c          770 g         1739 t
ORIGIN      
        1 taattttaat gctactccat gaagcaacaa tcaatttgtc agtgaaattt cactctaacc
       61 agtctttcgt aatagttttt gggaagcttt cagaattcaa acgtttttca cttgatactt
      121 tagcagctat tggtttttcg ataaaccaat tttttacata gacaaaaatc aggttctgga
      181 tattttcaac tttttcggtt tttaattttt gataggcttt taacagtgtc gtgtataatt
      241 cttgttctgc attgacataa taactatcga gttcttcaaa aattattgaa ataccacttt
      301 ctgtttcggc ttttttcttt gcattatgaa tagtctggac agttttttga gcttcactgt
      361 aacttgaaga aaatgttttt attaaattta atactcgttc agggataaac gtagcaatgc
      421 tgtcgtttgt cataattgtc tcaaaattgt ctaacaacaa ttgatcttgt aggcggtctt
      481 tttcagtgtc tatattgtgt ctattagtgt ctaatatatt attattaagt tctggtatga
      541 ttttcatacc ctctgattta tcaacgtttt ggcacggtat gattttcata ccctcttgag
      601 ccccaaggga ttcagcgtct gatttttcgt cgagggtggg tatgattttc ataccctctg
      661 atttatcaac gttttcccta ttctctctat caaatttttc gagtaagtag atatcatttt
      721 ctgagacttt taattctgct agatatagtc tattaggatt atttttccct aactgtttgt
      781 taaatcccat ctgcttttgt aataataaac caaaggattc caactctttt ttgattgcta
      841 tcgctttttg tttttggcag tttaatacgc tacatagttc atttgtcgta taagtaaaat
      901 aaacattacc ttcttcatcg atttgccgtt tgctgattgc gatctctaat cttgctttta
      961 aaagcatata ggcaatctta gttgaatctt tcattttttt gtattcgtca gaaacaataa
     1021 agacttttgg aaattgataa aataattcgt tgtaaactct atccgcctta aaaaaattaa
     1081 aatcagtcat tgtagatcct ctactttcac gttgttttat ttatgtaaaa ttacctactc
     1141 ttttcgaaaa tagcaaagat aaataaaaaa tgcaaacgca ttaaaactgt tttttaatta
     1201 aatactacct aaaatcagaa ataaaatgaa ttatgtacta cctttaaagt cattaagaac
     1261 tactttaaat aaatattatt tataattaaa tactaccaga agaacgtatg aataaaattt
     1321 agttttaaaa ttaaagataa ttaagtacta ccagtgtcaa aacaaaaaat aaattaagaa
     1381 ctaccttatg catataaaac atactaccat tactagtaat atatactacc ttatttcagt
     1441 agtatatatt atattcttct tataagacaa ggctttaggc tagaaaacaa tagattatat
     1501 actactaaaa aatacaataa gtactaccaa tgtcagatat atataataat tatgtactac
     1561 cattaaaatt aacttattta ataagatact actgtgttaa tctgaaaaaa attactttaa
     1621 agtagttaca tactacctgt taaattagat actaccatta atacttaaat tagatactac
     1681 ctatatttat attaagtact accgttctag gaataagata ataaaaaaaa actaccaaat
     1741 tgcaattaaa tactacttaa aatgtaaaaa ataagaacta cccaaattag gtagttctta
     1801 ataagagaat atcagataaa tttgtacaag tcataaaaaa attaatctta aatttttaaa
     1861 tatatatacc tgttaattat aaactacttt atttaggtag tacataatta acagataaaa
     1921 ctacatgctt gtagtttgtc tagctaatga gttgcatatg atttatataa aggtgtgcag
     1981 cataacttag ttgaagttaa gagaatagaa taaatatttt ttgttttgct aataattaaa
     2041 cactacctaa atatggtagt gtttaattat ataatattca ttttgttaca aataatcgta
     2101 ttataaaagc ataacaattg ggataactat tttttagatt atttaagtac tacttaaatt
     2161 aggtagtact taaattttgg tttaaggtag taggtaatta agaaataata tatatcttct
     2221 tttataatag aaggggagag gcctagttaa aaacagcgac aattaataac aatgtattta
     2281 atgcttcaca aaatccattt ggtggacaat gcattgttat tagcgatgat ctaccgttct
     2341 aagtagtttt ttctattggt tgcattcgtg actaattatg gtataattat gagtaacaaa
     2401 gaggtatttc attatatcat caaaccccat cagcgtgtag gctgatgggg ttttttttac
     2461 gtttctatat tatagaatta actggtaggc ttactgtctt ttttggcaat tttgcaatca
     2521 agccacttgc agatgtagtg tgcagttacc tctgcaaatg gcttgattgc aaaaacaaaa
     2581 tcaaaaaggt atttcattat atctcctcct ccccttgcca gattaggccg gggtagcaga
     2641 cagcaaaact attataacat aactgaaata cctaaatgac atatacagat atgataattt
     2701 atgtattgat ttttctaaca aaagcacata ttttaatata tactacctat tttaggtagt
     2761 atatattttt agatacaata tcgatcagtt ttgaggaaat gaaattctag atattttttg
     2821 ctacatagag ttactttcaa tattttttaa cgtaattagg cagaagtcac tcatcctcta
     2881 taggtttgtg atgaaattgc tattgatcca tttaagtaag agcaaaaaac tggaggttta
     2941 tcaggtaatt ggtctaatgg atttaagtat gcggtcaccg tgtatagagt tgcttatgag
     3001 attcaagaaa ataaagttat tccagtattg ctatatggaa cacataacaa tttttatgaa
     3061 ttagccgaga acactcgcaa ctttattcgt gtagatgaat cggcttcggt tgagttataa
     3121 ctttagctat atcaatcaac cgaccgacat atgttaaatc tatatataat aaaaatacct
     3181 acttaaatgg atgttaacgg aagtgtatgg tgagtagttc actcgtattt tgatatcgtt
     3241 tgtatatgtt ttcagcgttc taagtggctt attttgtcca ataataatat ggagtggaat
     3301 cccttaagaa acaccgttgg caattgtcgt tataacatgt tataatttat ttatcaaaaa
     3361 ctaaaagaag tgtgataaca tgggaaaaga actaaaggtt agaaagatcg gcaatagcgt
     3421 gggagtgatc cttccaagct ctcttggatt aaaaagcgga gacactatcc aagccaaaca
     3481 agaaggcaac ttattcatat tagatactac tcaaatagca aaagaacacg atcgcaaatt
     3541 gattgaagaa agtttccaag actttgagaa aggtttaaca gtttcagaaa ttgaaatggt
     3601 aaaagctttc ggaaaatacg gctggagtga atagatgaaa gagtacacgc tagtttattc
     3661 taaaagtttc cagaccagcc ttgaaacgat aatagaagaa tgggaaaatg agcttgctct
     3721 ttctccagcc agcataaaaa gatttgtagg tgcgatccag aaggctttag aactaaccaa
     3781 aaattttcca actatgcatg aggaagtttc cgaaatctat ggaatggata tcccaacata
     3841 tcggatcgtg atcggcaaaa gctatgcctt attttatcgg atcgaccatg aaaaaaaaga
     3901 gatacttgta ggacgtattt tccaatctaa acaaatgaaa ctaggctttt aacaataatt
     3961 aggcaaaagt cgctcatcct ctacaggtgg gtgattaatg ccgctcgata tggttgttaa
     4021 ccgtcggtta ctcgaagatt gatatataag acttagtacc tttttatttt gctccatgtt
     4081 atggccgaag gtctaaagat acaatcatta gtctagagaa aaattaagaa ccgtaggaac
     4141 tacggggatc gcttggtgaa taagagacac tgctattagt gaaaaaataa gatatcaagt
     4201 acgctctatt cccaaaaatt tcgtgacttt actcatgaga ggttcaagta gtatattttt
     4261 aatcaaaatt gacgtaactt ttaataaata ctacctaaat aaggtagtat ttatttttgt
     4321 gttttaaaag aggaagtcca atatatctat gggaaacttc ttaaatatta tgttggtaca
     4381 tgaaaatata catccctgct agactattta caacatttta ctttgtacgt attcttgttt
     4441 taaaaggaat gggataaaat ataggtacaa taactatttg atgctaaata taattaatgg
     4501 aggagagata gtgaacatac ttaatgaaaa aagatatgta tggtttgctg gtaaaaaata
     4561 tattactact ttcaaaagat tatccgagtt aatgcagcag actgttaaaa gtgctatgcc
     4621 tgaaggatac aagtttaatt ttgttcaatc ttcagtatct actgaaagta tctatttttc
     4681 aattctttct gagagtaggc tttttcattt tagaataagt tctcatgtaa agaaaactga
     4741 tgttgatata tatacgttcc gtacaaataa ttataaaaat ttaaatgatt tgagtagaag
     4801 tatcagtgaa tatctcaagg ggaatagcta tctcaagatt cgattttcac atatattaat
     4861 tttactacca tatttgaatg agaatttcac cctaattcaa gtagataagt ctttcattgc
     4921 ctcaggtaat caaggaaata tga
//
LOCUS       DANKMN010000119         4931 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000119 DANKMN010000000
VERSION     DANKMN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            84..971
                     /locus_tag="PW224_002758"
     CDS             84..971
                     /locus_tag="PW224_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2909381.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1111..2037
                     /gene="cysK"
                     /locus_tag="PW224_002759"
     CDS             1111..2037
                     /gene="cysK"
                     /locus_tag="PW224_002759"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL2909382.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2273..2719
                     /locus_tag="PW224_002760"
     CDS             2273..2719
                     /locus_tag="PW224_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2909383.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2992..4344
                     /locus_tag="PW224_002761"
     CDS             2992..4344
                     /locus_tag="PW224_002761"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL2909384.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4515..4802
                     /locus_tag="PW224_002762"
     CDS             4515..4802
                     /locus_tag="PW224_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909385.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1694 a          813 c         1030 g         1394 t
ORIGIN      
        1 attaaaagta taatttaatt aattaagttt gttttaagaa gagaactttt tattaagttt
       61 ttttattttg aggaggaatt tgaatgcaag agctgaagac ccgcagtgcc cggcaccata
      121 gacctaaaag aaaaatggtg atcaataaaa aagcgattgc tcctttaatt ggaacgagtc
      181 ttttagttgg gccgatgatg gttacagcgg taccgcaaca tgttcatgca gatgaacaag
      241 gatctcaaac agtcgctgat cctcaagcgt ttatcgatca aatcggctgg tctgcgtcag
      301 aagttgctgc aagtaatgac ttgtatgctt cagtcatgat cgctcaagcg ttgttggaaa
      361 gtgcatatgg cacttccgga ttagctagtg cgccaaatta taatctattc ggtgtgaaag
      421 gaagctataa cggacaggtc gtttatatgg caaccaaaga atatgtaaac ggacaatggg
      481 taacgatgaa tgagccattc cgaaaatatt cttcttattg ggaatccttc caagaccatg
      541 cgaatgttct tcgttcaacc agtttttcca cgggcgatta tcattatgct ggcgtgtgga
      601 aaagcaatac aacaagtttt tatgatgcaa cagcgtactt gacaggtcgt tatgctaccg
      661 atccaaatta tggggccaaa ttgaactcat tgattaatac ttataattta acccgttttg
      721 atacgccatc aacaggttcg gctacaacaa ccacaagtac aacaacaacg actactacga
      781 cgacttcaac gtcatcatct tcaacaacag atgtaaatac aaataatggt cagacatata
      841 cagtcgtgtc aggtgatacg ctatgggata ttgcacaaaa atttggcatg tctgttgatg
      901 atctaatggc gaaaaatggc atgtctatgt ccagttattc tttattagtt ggtcaagaac
      961 taaacgtttg atttagataa aaaggcaaag aaattaccat atatggtata tctttgtctt
     1021 ttttttgcta gtcaaagacg aatcactcgc cataaatgaa cagattgtat tatgataagt
     1081 aagaagagca aagacaagga ggaattgaag atgacagcta tctataactc agtcaccgaa
     1141 ttgattggca aaacgccaat cgtcaaatta aataaaatcg taccagaaga ttcagcagat
     1201 gtttttgtaa agcttgagtt ttttaatcca ggaggaagtg taaaagatcg tattgcttta
     1261 agtatgatag aaaaagcaga acacgatggt ctactgaaac caggtgatac tatcattgaa
     1321 cctacttctg gaaatacagg aatcggattg tcgatggtag gagttgcaaa aggatataaa
     1381 gtaatcatcg tgatgcctga gacgatgagt atcgaaagac gtttattgat gaaagggtac
     1441 ggcgcagagt tgattttgac tcctggtgcg gatggtattt ctggatcaat cagagaagca
     1501 gaacgtttgg caaaagaaaa cggctacttt ttaccattac aatttgaaaa tgaagcaaat
     1561 cctcttgtgc atgagaagac gactggtcct gagatacatc aagcatttgg agtaaatggc
     1621 ttggacgcgt ttgttgcagg tatcggtaca ggaggaacga tcactggcgc tggccgagaa
     1681 ttaaaacgtg tgtatccgaa aatcgaatta atcggggtag aaccagcaga atctgctatt
     1741 ttagaaggga aagaagcggg acctcacaaa atccaaggaa tcggtacagg ttttgttcct
     1801 aaaacactgg atacttctgt gtatgataaa gtcctttcta ttagtggaga cgaagcgatg
     1861 gaaacagcta gagaagttgg gcgaaaagaa gggattttag taggcatctc atctggcgct
     1921 gcaattgctg ccgcgctaaa agtcgcaaaa gaattaggaa aaggcaagaa ggtattagct
     1981 gtggttcccg acaatggtga acgttattta tctacagccc tttaccaaga gatataaggc
     2041 acttggtttt tctccagata ggtttatgta aaaaactttt aaagatgaaa agaacacaca
     2101 aaaatttatg atgagaaaaa ttatttgaat agagacgtag aatagtgaac gcgactgttt
     2161 tacgtctttt ttcattaaaa tacaataaaa ttgaattttc ttttcatgtt aagtggattt
     2221 taaaatgtaa aaatagtatg ataagacgag aataacaacg tggggtgttt acatggaaaa
     2281 taagttagtc gaagaggtta tcgaaaagtt aaaaaaagag aacattcgaa tcacacctca
     2341 acggtacgcg gttttagaat atttgattga acatcattcc catccaacgg ctgatgagat
     2401 ttataaagca cttgaacatc ggtttccaaa tatgagtgtt gcgactgttt acaataacct
     2461 tcgcttattt acttcgattg gatttgttca agaaatgaag tacggcgatg cttctagccg
     2521 atttgatttc agttcaaagc gtcattatca tgtaatttgt gaaaactgcg gtaagattgt
     2581 tgattttcat tacccagggc ttgaagatat agaaatggct gcaagcagac tgactgattt
     2641 tgagattaat gaacatcggt tggaattgta tggactctgt ccggattgta aagagtcagc
     2701 aaaaaaggcg caaatgtaaa aacagtttct acaagtgtta tacctaaaaa aagctagctg
     2761 tgtaaaaaaa acgctagctt ttttattttt ttgtctttta ttcacaatct tagatataaa
     2821 agtgaatatt attaaaataa gaatcttgta gatattgggt tcttatgctt gaaaaggaat
     2881 gaaactacaa acaaaccttt gtgaattcac aagcattaaa ctgttgtatc cgttttattt
     2941 ctgttatagt agttttgtcg aaggacaaaa tgttaatcgg agggaccata aatgaaagta
     3001 gtagtaattg gctgtactca tgcaggtact gcagctgtaa aaagtatttt aaaaaatcat
     3061 ccagaagcag aagtgacagt atatgaaaga aatgacaata tttctttctt atcttgcggt
     3121 atcgcacttt atgtcggcgg agtagtaaag gacccagcag gcttgttcta ctcaaatcct
     3181 gaagaattat cttcattagg agcaacagtt aaaatggaac acgatgtaac agacgtcgat
     3241 acagaaaata aaacagtaac ggcaaaagat ctgaaaacag gagaaacaga aacggtttct
     3301 tatgataaat tagtgatgac aactggttca tggcctatta tcccaccgat tagtggaatc
     3361 gatgcgaaaa atgtactcct atgcaaaaac tacaatcaag caaatgaaat tattgcacaa
     3421 gcgaaagaag caaaacgtgt agtaattgtt ggtggtggat atatcggtat tgaattagta
     3481 gaagcttttg tcgaatctgg aaaacaagtg acattgattg atggattaga tcgcatcttg
     3541 aataaatatc tagataaacc attcacagat atcttagaaa aagaattgac agaccgcggt
     3601 gttactcttg cgctaggcga aaatgttact gaatttatca cagatgaaga aggaaaagtg
     3661 aaacaagtag ctacaccaac agatacattt gatacagata tggtcatttt atgtgtgggc
     3721 ttccgtccaa atacaaaact gatcgaagga aaagtagata cactgccaaa cggggcaatc
     3781 aaagtgaatg aatacatgca gacaagtaat ccagacattt ttgcagctgg agactctgca
     3841 gtagtcaact acaatccaag tggcacgcaa aactatattc ctttggcaac aaatgccgtt
     3901 cgtcaaggtt tgcttgtggg aaataacttg acagatcata aaatggcgta tcgtggtact
     3961 caaggaactt caggacttta cctatttggc tggacgatcg gttcgacagg tgtgaccaaa
     4021 gaaagcgcaa ctttgaacga tctagaagtc caagcgactg tatttgaaga taattatcgt
     4081 ccagagttca tgccaactac tgaaaaagta atgatggagc tagtctatga aaaaggaacg
     4141 aatcgtatcg taggtgctca gttcatgtcc aaatacgaca ttacacaatc agcaaatacc
     4201 atgtctttag ctgttcaaaa taaaatgact gtagaagatc tcgcactttc agatttcttc
     4261 ttccaaccac attttgaccg cccatggaat tacttgaacc tacttgcaca agcagcgcta
     4321 ggcgaaatcg aaactgcaca ataaagaaaa agggttgtga cacaattttg tcacgaccct
     4381 ctgtcttttt ttgtgaaaag tacaattagt tgtctttctt cgaatttttt gtcacaataa
     4441 gcagcaaaga taaagctgtt acataaaaga acaagcagct ttatttgatt ttgaaaagaa
     4501 aggtgagaaa gaagatgaat aaagaatatt ttgtccaaca agatgcattt ttagaagatt
     4561 ataaaagtgc ttcgaagaaa ttaacgatca atattgatga gttggcagat caagtactct
     4621 ctcagttgaa tgaaacgcaa gaggaatatt acacgatgcc agcaaatgac gccaaagata
     4681 atgtttctca tcgttttcct ttcagtagac gcatctactc agatgaaaat gacggcacat
     4741 tgaatacaga gtacacatat gaaggtcagc catatgtgct agaaactgat gatgatcttt
     4801 aaagacagtt tttgaaaagg ccgctcatcc ttaagtgatg acaaaaaatc atcacaatta
     4861 ccgatgactg tctctttact atcttttaat cagaaaaagg ggttatgaca agctttgtca
     4921 accccttttt t
//
LOCUS       DANKMN010000120         4900 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000120 DANKMN010000000
VERSION     DANKMN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="PW224_002763"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="PW224_002763"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="PW224_002764"
     CDS             complement(227..697)
                     /locus_tag="PW224_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL2909386.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="PW224_002765"
     CDS             complement(856..1314)
                     /locus_tag="PW224_002765"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL2909387.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="PW224_002766"
     CDS             complement(1485..2630)
                     /locus_tag="PW224_002766"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL2909388.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="PW224_002767"
     CDS             complement(2643..3560)
                     /locus_tag="PW224_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL2909389.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="PW224_002768"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="PW224_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL2909390.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4652..4822
                     /locus_tag="PW224_002769"
     CDS             4652..4822
                     /locus_tag="PW224_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909391.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1531 a          891 c          931 g         1547 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacct
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc
     4801 gaaaatattt tttcatcact aaatagtgtt gaatttatgt ttttatgtat aaatagcttc
     4861 taatagttcg ggtattttgt ttttataacc atttttattc
//
LOCUS       DANKMN010000121         4883 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000121 DANKMN010000000
VERSION     DANKMN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            509..2353
                     /locus_tag="PW224_002770"
     CDS             509..2353
                     /locus_tag="PW224_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2909392.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2409..3875
                     /locus_tag="PW224_002771"
     CDS             2409..3875
                     /locus_tag="PW224_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2909393.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3912..4754
                     /locus_tag="PW224_002772"
     CDS             3912..4754
                     /locus_tag="PW224_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2909394.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1605 a          785 c         1028 g         1465 t
ORIGIN      
        1 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
       61 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
      121 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
      181 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
      241 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
      301 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
      361 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
      421 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
      481 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
      541 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
      601 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
      661 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
      721 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
      781 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
      841 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
      901 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
      961 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     1021 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     1081 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     1141 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     1201 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     1261 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     1321 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     1381 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     1441 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     1501 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     1561 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     1621 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     1681 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     1741 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     1801 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     1861 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     1921 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     1981 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     2041 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     2101 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     2161 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     2221 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     2281 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     2341 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     2401 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     2461 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     2521 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     2581 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     2641 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     2701 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     2761 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     2821 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     2881 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     2941 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     3001 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     3061 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     3121 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     3181 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     3241 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     3301 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     3361 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     3421 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     3481 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     3541 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     3601 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     3661 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     3721 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     3781 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     3841 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     3901 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     3961 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     4021 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     4081 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     4141 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     4201 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     4261 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     4321 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     4381 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     4441 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     4501 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     4561 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     4621 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     4681 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     4741 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     4801 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     4861 ttaacgtaag ataataaaaa atc
//
LOCUS       DANKMN010000122         4712 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000122 DANKMN010000000
VERSION     DANKMN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(673..1491)
                     /locus_tag="PW224_002773"
     CDS             complement(673..1491)
                     /locus_tag="PW224_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2909395.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="PW224_002774"
     CDS             1652..2341
                     /locus_tag="PW224_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2909396.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="PW224_002775"
     CDS             2355..3857
                     /locus_tag="PW224_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2909397.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="PW224_002776"
     CDS             3870..4346
                     /locus_tag="PW224_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2909398.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4443..4553)
                     /locus_tag="PW224_002777"
                     /pseudo
     CDS             complement(4443..4553)
                     /locus_tag="PW224_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1590 a          673 c          829 g         1620 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4501 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4561 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4621 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4681 tatataccaa atatagtaaa atgctgaaag at
//
LOCUS       DANKMN010000123         4694 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000123 DANKMN010000000
VERSION     DANKMN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            81..1030
                     /locus_tag="PW224_002778"
                     /pseudo
     CDS             81..1030
                     /locus_tag="PW224_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(1075..1708)
                     /locus_tag="PW224_002779"
                     /pseudo
     CDS             complement(1075..1708)
                     /locus_tag="PW224_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(1968..2516)
                     /locus_tag="PW224_002780"
     CDS             complement(1968..2516)
                     /locus_tag="PW224_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909399.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2517..3374)
                     /locus_tag="PW224_002781"
     CDS             complement(2517..3374)
                     /locus_tag="PW224_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2909400.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            complement(3660..3947)
                     /locus_tag="PW224_002782"
     CDS             complement(3660..3947)
                     /locus_tag="PW224_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2909401.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3937..4266)
                     /locus_tag="PW224_002783"
     CDS             complement(3937..4266)
                     /locus_tag="PW224_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2909402.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(4343..4489)
                     /locus_tag="PW224_002784"
                     /pseudo
     CDS             complement(4343..4489)
                     /locus_tag="PW224_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(4541..>4694)
                     /locus_tag="PW224_002785"
     CDS             complement(4541..>4694)
                     /locus_tag="PW224_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2909403.1"
                     /translation="NKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKV
                     LLGIQA"
BASE COUNT         1417 a          907 c          688 g         1682 t
ORIGIN      
        1 agaggacagc atatcgatat tgcttccttt tttttgctat ttatttaagg gatagaagtt
       61 tagaaaggac tgtttataag atgaaaaatg atatagtgac ttatgcaaac acaaaatgtg
      121 aagaaattgg catcagtaat ttagaagagt gcttttttga cacaaatgac ctgtcgcaat
      181 ttttaagaac gggaagaaca aatgccagta aacttgccaa tgacttagtt gcaaagaacg
      241 ttctatttaa aatcaatact cgtccagtgt tatttgccat caaagagcgc tttgaagagc
      301 actttttgat tccggtaaaa gatgtatacg aaagtatatc tgaattaaaa acagacttgg
      361 agcccaattc tacagattcc attctacaaa agatgattgg gtatgatatg agcttatccg
      421 atgcggtgga acaaatgaaa actgctgtct tctatcctgg aagagggctt cctatgatga
      481 tcactggaga tactggagtt ggaaaaagtt tttttgttca tactgcttat cagtttatga
      541 aaaaagtgaa cgttattcat gagaatgctc cttttaaagt acttaattgt gcgcaatacc
      601 ataataatcc tgaattattg tcggggatac tatttggcta tgttaaaggt gcttttaccg
      661 gcgcagatga agatcgaaat ggattgttgc aagatgcaga cggtggagta ctgtttttag
      721 acgaagtaca cagattgcct cctgaagggc aagagaaact atttgtttat atggatggcg
      781 gaacttattc tagagtcggt gaaactatcc atcacgcctc caatgtacgt ttagtttttg
      841 cgactacaga aggcaaagag gattttctgg aaacctttat gcgaagaatc cctatacata
      901 tccatatccc aaatattgag gaacgaggta ttttagaaaa aaacaaatta tcgaaaatac
      961 tctctttagc gaaagcaaaa actttagcaa ggctattgat gtttctccta aagtcactag
     1021 tgttgcataa atatagtcag aatattctga attattattt tcttgaaaaa agtaaaaaaa
     1081 atcgtatccc aaactttcat ctacatttcc agaaagatct tcatgttgat aatcaattcc
     1141 tgaatcaatg accgctacct tcacatcctt agaacccttg gtttcttccc aagcggatgg
     1201 catgctaatc tctcttaaat tagcttgttt actccaatta ggatcgttat cgatttccta
     1261 aagaatcagt tgtatttggc tcctcaacat cattcaacgg ctctagttca accacatagt
     1321 ttggctctgc ataattgacc atatcagaag cattaaatgt tcttactgcc tcttccacac
     1381 taatttcttc gttcaatttc actaataata cttgatctcg cttattataa tcgctttcat
     1441 ttgtcctaag agtcaaatct ttttccacca taacttttac acccaataaa ttttgcggtt
     1501 tatttttctc tgttgaaatt ctttgtgtag cctttaaact gacaattagc tcattgggta
     1561 catactctat ttgagagtca tctaattggc tcataatcct ttctttttgg ttagtatctg
     1621 cagttacttg tttagtatga gccatgaacc caaacatact caccccaaca accaatccta
     1681 aatatatttt ctttttaaac tttttcatct acatctcctc ctaatagttt tattttagag
     1741 ttagctctat tacttattta ctaactcaac aacagattat catacactta ttaatgaaat
     1801 cacaatatct cattaaaaca caccctaaaa tcataaccgt tcctatgctc attttttgga
     1861 ttttttaatc acgtaaccag aaaaataaat cccctctatt tcacgcaaat caaagaagaa
     1921 ctaagtaact accgtttaaa ggtagttact tagttcttct ttgattatta tctaacttta
     1981 ttattcattc cccacccttt gcgcagtgag tcttcggtat cttgttttat ctgactggta
     2041 gttcttatta tttctccacg atcatctaat tctggaacaa ttggatattt ttctctgaac
     2101 aagtctagtt ctttcaccac gtcattaaac aaatcttgtt tatcttcttg cgtaagattc
     2161 tgtttaatat actcatctaa agcttttcta agcaattcat ctcttgtcat attagtctca
     2221 tacttttcta ctaaaactct ggaagtcgca tttaacaaag tcgacagttt taaaggatgc
     2281 ttggtggaat aaaccttatc aaactttctg attggtgcgc caactttttt tgttcttcct
     2341 ctactctgtc tttcaacatt ggaggaagtt tttatttctt gaacttggtg cacttctaat
     2401 tgcttatttt cagatggtgt aattgcttgc tgctttttct ttgcttctgc ttcttttttt
     2461 attttggcga gtcctttgtt gttcccaaaa atatcaagtc ctttgatgtc agccaattac
     2521 tccacctcca cataccaatt ctcgtgttct tccaattctt tcaaaatact gataaataat
     2581 tcgtggcttt tgcgatccca ttgttttaag agacctttct ttgtcttctc catcgtaata
     2641 cctgatccgt cgtatcgttt taagcgcgca tttttgagta caacattttt aatcaaatgt
     2701 tccccataaa tctcttgggc ttgtttatac atttcctgat ctactgcgtc accgctttcc
     2761 aacatgaaag gaataacacc agcaacatat aaatttgtat caaattcttt cacaaaaaag
     2821 tccatatact caagatattg catcgcattt ctcatcgatt ttacttgggt ctgtaagacg
     2881 acgattgccc aatccgcaat gtacatcgcg gatttactat atacagaaat agaaggtggg
     2941 acatctaaat agatgacgtc aaactcatca aataatggtt ctagcttttc tttcatatat
     3001 ttgaattggg ataatgaatc attatattgg tctttgatcc aatcatcata catttcaaag
     3061 gacatgtctg ctggaagtat atagaggtta ttgattactt tatagacagc ctctttaggt
     3121 ggaagattgt ttatcaacac gtcagtcaca gtattttcgt aaacaatcgg ttcaccttct
     3181 ccgcctcctt cttcgttagt gaagttcata tgcgttaagt tcatcacatc agaagtattt
     3241 ccttgcatat ccaagtcaat tatcaaggtt ctttttcctt cattaactgc tgattcaaac
     3301 cctaacattt gcgcgcacgt acttttacct acgccacctt taaaattacc gattacatat
     3361 ttcttcgcta ccacgttcct acctcttttc ttttagcaat attcatttga tactattata
     3421 acatatttat ttttagttac taccaaaaaa aagatatata tgtactacct tataattaat
     3481 aagtaaaagg tagtacataa ataaggaaaa gctgaaatat attgatttat agctttcctc
     3541 aaagtaatga cgttatttaa tttatcgaga agcagagaat actcgcgact ttagtcatgt
     3601 agacgaatca gattttcgat aaaaaagaag ccgtccttct taagaacgac aacaaatcac
     3661 tagattaatt ttttcaactg ttcataaaag ttttcatgcg ttccacataa caatactgga
     3721 ataaccttgt tttctataat ctcgtaagct actctatatg ttgtgcccgc atatttgaac
     3781 ccactagacc aaatacctga taaatcccca gttttttgtt cacccttata tggatcaatc
     3841 gcaatttcat cataaattaa attcaaaaat ttttctttca atggcttctc ttttagcttt
     3901 tttaaatact taatgacatg ttttgaagga acgatttcat aagccaattt ctgttgccaa
     3961 ctcctcttta gtcattactt tagaattagt aagtgtatct tcagcaatat ctctaaagga
     4021 agaatgcatt ttgttttttc tattcttaaa ttctacaaga atctcatctt tatcgtagcc
     4081 ttctgcaatt atatcagaca aaatatcttc gctaaaatca taaaattctt tttgtggttc
     4141 aacaaattca taaagaatac cattctctac caactttgct tcaacttcga ctccattagg
     4201 aacattaaaa tctttaggta ctgtcaacat gattgaattg ccttgttttc ttgtttttgt
     4261 aatcatttaa atccctcatt tctaacagta tgtaattact tagtaaatac attataccac
     4321 gagataatgt atttacaagt aattactgtg actcgtaatg cgtccttgct gaataaattt
     4381 caaccacgtg ttcatcttca tctaccttat aaacaacccg gtgttgtcta ttcaatcttc
     4441 taaaatacct accttcatgt gtaggtttta acttctcaaa tgattgggtt ctgttgcaaa
     4501 gttttaaata aagaataaaa tcccttacgg tatctatgat ttaagcttgg attcccaata
     4561 ataccttgat ttcagtacag accgaaaacc cgaagagagt gccttctttt cgggttttct
     4621 tatataatcc tcgaatggct tccatgcctt taatcgtggt agaggcagtg cgtaaacttc
     4681 gatagaattt attg
//
LOCUS       DANKMN010000124         4587 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000124 DANKMN010000000
VERSION     DANKMN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            186..2855
                     /locus_tag="PW224_002786"
     CDS             186..2855
                     /locus_tag="PW224_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL2909404.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3566)
                     /locus_tag="PW224_002787"
     CDS             complement(2946..3566)
                     /locus_tag="PW224_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2909405.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="PW224_002788"
     CDS             3680..4219
                     /locus_tag="PW224_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909406.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="PW224_002789"
     CDS             4216..4554
                     /locus_tag="PW224_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HDL2909407.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1518 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggaggaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgac
//
LOCUS       DANKMN010000125         4477 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000125 DANKMN010000000
VERSION     DANKMN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(832..1125)
                     /locus_tag="PW224_002790"
     CDS             complement(832..1125)
                     /locus_tag="PW224_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL2909408.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1528..2706)
                     /locus_tag="PW224_002791"
     CDS             complement(1528..2706)
                     /locus_tag="PW224_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2909409.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2810..3124)
                     /locus_tag="PW224_002792"
     CDS             complement(2810..3124)
                     /locus_tag="PW224_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2909410.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3231..3446
                     /locus_tag="PW224_002793"
     CDS             3231..3446
                     /locus_tag="PW224_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909411.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3746..3988)
                     /locus_tag="PW224_002794"
     CDS             complement(3746..3988)
                     /locus_tag="PW224_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2909412.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3975..4403)
                     /locus_tag="PW224_002795"
     CDS             complement(3975..4403)
                     /locus_tag="PW224_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2909413.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1418 a          841 c          663 g         1555 t
ORIGIN      
        1 ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt attattttat
       61 tgatgtatag aaagtttggg gctttttttc tctttttatt ctaaggttat tttagtcttg
      121 tcctttaata caaagatttt atttttaaat aaaaaatact ctttttttac gattattgaa
      181 aaaaaaagat aaaacttgat taattgaagt attgcttaaa tttggaagct atgagggaag
      241 aataaaagag ttaggacggg attatctact atttttcttt gttttaaata tttgttatct
      301 ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt ccctcatcac gaataaacgg
      361 tgttcaatta gctgatcttg tattcactta aaaaatggta aagtacgagt tgctattccc
      421 cattttcaat tttctaacgc tcaggacaaa atactgtttt cacaacctca ttggaataaa
      481 agcatatata ttgtttcaag aacgaaatct tgttatcttt ttaaagggca tctgtccaaa
      541 atctaatttt aggaggtacg ttttggtatt tattcgtaca aataaaagag aattatcttt
      601 attcatgtaa cgcatccaat tgattttttc atgtacgctc ctggaacgat tttactgacg
      661 attgcttatt taactgcgaa tcgctcatta gcttttatca tagctatcca tattttgaat
      721 aacgttcttg gctttgtatt atagatacag atgacaaaaa aagttcaagt atgcggtgta
      781 gaaccaatat cgcatacttg aacttttttc aatgagtata aattaaaact tctaattttc
      841 aattcccatc atctgatatg cccgaccgtc tgaacttctt ttgaaaaagc caaatgtgat
      901 tagataacgt ctttgttcga cataatcgcc actagaaaat acgttcagca gtatcgaatt
      961 aacctctgtt tctgtataag tcactccatg gtcaaagcgt ttcaagactt ctttcaatag
     1021 gatgtatttc ttttttgttg aacttggtac ttgttttact tttccatcat tcatgaatgt
     1081 gtctaaaact ttcatctttt cttgctccgt ataagtataa accatcttct ttctcctttt
     1141 gattctaaga atactccatt tatgttagaa tattctggat tttttattaa agcggttact
     1201 cttaagaaag atattatcat agtgctcttt tttttactac tacttatagg tagatttatt
     1261 ctaatccatt attctcccct accaataatt aaagaaaaaa ctggatcagg gtgttcaaat
     1321 tacatgctaa gaaaaaagaa cccttgaata ccaagggttc tttctcagtc tgataacaat
     1381 gattaacgac ggatttcttt gatacttgtg aaaagtcgtt aaagtattga tataaaagga
     1441 tttttattac ctctgactac atattgacta atttgttaaa aaatcgtttt tcaagaatcc
     1501 tgtaatacca gtgctttcac cgttttttta actctctaaa gaactaaaag caatcagagg
     1561 ttttaccttt tcatttgacg ttaagtggcg ataaattttc gaagttgttt ggtcagtatg
     1621 gcctaagcga tctttcaaga cttcccagct acagtgttcc gctactaagc ttgccattgt
     1681 atgacgagct aaatgagtat gaaatggctt attaattcct gagcgattca cgcaccgttt
     1741 gacgtgatca tttacatact ctcgacgata aggaacacca cgtttactag aatcaagata
     1801 cacaaacaaa tatgtttctt ctataataaa cctgtaatgt tgcctcatct cacccaaagc
     1861 cttaatcaac tgttgtagct tttccataac tagaggagtt actagaactc gtctagcaga
     1921 actaactgtc ttagtagttt caattcgaaa atccttactc ttacctttaa ctgccgtttg
     1981 cttatagaac agaatttcat ttcttttgaa atctatatct ccaggctgta gagcaagcgc
     2041 ctcgctgact cggcagccag tgtaaaatag aacatggaaa agtacagaca tcggtaattc
     2101 ctcatgaaca atgccataat tcaaaaaagt tctcaattca tcgacagtaa gaaacttgtc
     2161 ctcaatatta tttatggctt cttttaaatc agttaccgtt aactgatatt ttggcattct
     2221 cacagacttc attggatttt cacttatcaa attctttctt tgacaatatt taaaaatcat
     2281 atttagaact tgaataagtg attgtaaact atttttggca tagccaacat ttgttccatt
     2341 aacgccacat tcctttaagg taaaaagcaa ttcttgaagg cactctggtg tcaaatcaga
     2401 caacttttta tcaccaatcc atcgaacaat aatttttaat tgttcctctc gcttatcata
     2461 ggttatttcc ttttgttcat ttaacgaacg gtaatattgg aaccattgct cacctacaac
     2521 actaaacttg tcattgcttg caacaatttt tttcttaagc ctttgttgtt tctccatttc
     2581 cagataagcg agttgcgcct cacgttttga attaaagcca cgtcttgttg ttcgatatgc
     2641 ttgaaagtac catgtgatcc taccagtaga actgatttgc ttcttgaatc gatctgtttt
     2701 ttccatttat tgattcctcc tattttgaag gaacccaaca agcagaaacc ctttggaaat
     2761 ctttctatct atgtcatcat tgtaccgcat cttagaaaga aaatgtaact tataaagatc
     2821 gaagtagtcg caatttttct cgatagtcat tcgaatcact ctttctcatc cattctaaaa
     2881 catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca gggaggaaac tcaccaagtt
     2941 ttactaaacg gttcagtgta ctatcactta tctgaaatag tcgctttaaa tcagtatttg
     3001 ttaataccag aggtaaatga ctgactgcaa gcaatttttc cgtgtaatct tccagttttt
     3061 tttgtaaaat tgaatagaac tctatttcca gagaattaac atttgtttta acttccaact
     3121 ccatcaccct tctctctcaa aatcaaaacg accaccacgg taccccttaa aattgtaaaa
     3181 aaacactcca gtactccatc aaaacggact agaaaagagg aatttttaat atgaaacaag
     3241 gaattgcaga cataaaaata attaaagaaa ttcttgaaaa atctacggct aatgcaatag
     3301 cattcggaac aggaatcaac ttgagtacag taaaaaaact taaaagtgga gagagagctg
     3361 aagaaaaatt aaatcttgcc gatgctatta aaatcacaga atttggcatg aaaaatatgc
     3421 caaccaaaat cgaaatatgg aaatagtagt ttttctgatc tatcagctgt tttgccaaag
     3481 cacataggaa tctcacctcc ccccagtaat ggcgggccga tttggttacg gaagtatcat
     3541 tatcgtagtc gcgattctca tattaaaatt tgtcgcttaa aagctaacct tgtcatggcg
     3601 taactagaga atcaaaatct acatgtactg atagatttgc tattcatttg tcaaagagcg
     3661 ctaggctatt ttcagcctat ggaaatcctt tattttgacc cgataaaaaa cttccataga
     3721 atgaaaagga aagggaaaaa atactttaac gagttgtatc aaattcaagt atcttctcaa
     3781 tcaattttgt ttccagttgt ctacgcatat attcatctac atacataacc attccttttt
     3841 cttcttcata aactggtttc atggctaacc gtgaaatata attttgatat tttgacagaa
     3901 cgatggacat ggcaattgaa tcaccagaag ctgcggcttt gattgtcgtt agtggtatga
     3961 atggtatata tcgtttattt tgcttcatat ttgacaccca aatactttcc tagctcttcc
     4021 aaagccattt gtcgttggta ccaaacactg ccgagacttt tcccaataat atcgccaatt
     4081 tcacgatcat taagaccgat gaagaaggac ataaaaatga tttcttgtct ttgactaggt
     4141 aatgctgcaa tcgcatttgc caactcctca ttggaaataa agattttcat accaaggaca
     4201 ataaaagtac cactactatt caaatttggt tccaaataat agaactctgt acctaaccct
     4261 ttttgttcca atatttccat tgatatttgc cgttggcgaa atctagcgta cttcttttga
     4321 atatttaacg cctcattgca gactactttc ttacaaaaac tgtcaaacgt atgttgaatg
     4381 tgggtttcct ctgagtcgcc cataatattc tccccctcaa aaaattcatt caactcgttt
     4441 tacttctatc cctttccatt attactacaa tctagga
//
LOCUS       DANKMN010000126         4433 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000126 DANKMN010000000
VERSION     DANKMN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(543..2048)
                     /locus_tag="PW224_002796"
     CDS             complement(543..2048)
                     /locus_tag="PW224_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2909414.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="PW224_002797"
     CDS             complement(2045..2731)
                     /locus_tag="PW224_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2909415.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PW224_002798"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PW224_002798"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL2909416.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DANKMN010000127         4382 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000127 DANKMN010000000
VERSION     DANKMN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..1015
                     /locus_tag="PW224_002799"
     CDS             <1..1015
                     /locus_tag="PW224_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL2909417.1"
                     /translation="DFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNLNVAMAD
                     RERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKTIEQIKI
                     DNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKN
                     YSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEI
                     GKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNY
                     ISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFN
                     NIS"
     gene            1160..1465
                     /locus_tag="PW224_002800"
     CDS             1160..1465
                     /locus_tag="PW224_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909418.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            2082..>4382
                     /gene="esp"
                     /locus_tag="PW224_002801"
     CDS             2082..>4382
                     /gene="esp"
                     /locus_tag="PW224_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL2909419.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAG"
BASE COUNT         1607 a          594 c          817 g         1364 t
ORIGIN      
        1 tgattttaat gagatgtttc ttctatacat gtttaaaacc agatattact tatttggtcc
       61 atttagagca aataatattg ataaagattt ttttaaacta aaaatgaaca atttgaatgt
      121 tgcaatggct gatagagaac gactctataa ttcattgcaa aatttgactt tgtattcttt
      181 aggagacatt cgagatattc tgattttagt tcattatttt tttactggaa aaatagaaga
      241 tctattccat gaaccgttaa ttgaatatac aggaaatcta agtaaaacta tcgagcaaat
      301 caaaatagat aatctgttat cacaaaatta cgatccagaa atatatctgt ttctttatga
      361 aaataaaata ctggaatatg ttaaaaatgg agatattcga aatttagaaa acatggtttt
      421 taatctgagt aatggaataa ttccaagtgt tagtggagat acgattcggt ctgaaaaaaa
      481 ttattctatc attgtttttg aaaagttagc acaaacgtct ataacattag gaatggatat
      541 aatagaagcg tatcaaagta gagatgcttt gatacaagaa aacgaattag ctgtatcgtt
      601 acctgaggtc ttaaaagtga gggattctgg gattgtttat tatactaagg aaatcggaaa
      661 aactaagata gagcatcttt ctcccttaat ttcttctgtc gttcaattca tcggcttaaa
      721 tatctataaa agaattactg taaaggaaat agctaattat ttttctgtaa gtgaaactaa
      781 attaagagaa agctttaaga atgaaatgca tattacgatt tataattata tctctaagcg
      841 aaaaatttct acggctaaaa taatgttgaa atcaaaccat actatcagtg aagtttcttt
      901 aggtttagga ttttcagatt cttcacactt ttcgagagta ttcaaaaagt acgcaggtgt
      961 atctcctaag caatatcaat taggtttagt ggataatttc aacaacatta gctgagaggt
     1021 tttattttat tatgagacta tatcatttac gtagtttgga ggagttgagg aaattggatt
     1081 ttaacaaaaa attttttggg aaatttctga tttgagttgt ttcgtagaat ctgagcgtct
     1141 aagtaatggt aataatagtg tgttaaatga atatgacaaa gtagtaatta ataaagtaaa
     1201 aagtttatgt gaagaagcta gagaatctat cagtcatatt aatggtagca attttaagca
     1261 aacattattt aatctgatta agctagatgc taaaatatca agttatttat tctttttaat
     1321 acatgatgaa tttatggatt atcaaaaatt ggatcagtta attgaaagag aaagctggga
     1381 agattattac gtgggtctaa ctttttcaga aaagttaaac aactacaagt taatagatta
     1441 taagtatttg agtgaaagca attaattatt catgtagagt actagacact ttaaatatgg
     1501 acaattcaat tataaagaaa aagaatcaat aatttaatga ggctgtgaca aatgtcattt
     1561 tttgtttcgc taaaaacgag tgaaaaaatg ttgataactc caatcattcg tttaagattc
     1621 taggaatcat tcaggaatgc tgtgaaaccg atactttata attataaaga aggtgatttc
     1681 aaattccgaa gaaggaattt gaagtcacct ttttacttat gtcacagtct cattcagtaa
     1741 tgcgggataa ggctccttat tccgctattt gaatactctg aagtttaatc taagaaagag
     1801 taatattttg aaaacatgct tcatcgcata gaaatgaact taatttcaga aagaatacat
     1861 acatatcttt ttatatgttt tcagctgctg aatggttaaa aagcacaaat atccgaaaaa
     1921 aacatgaaga aatgtacttt ttttgttgta atgtgcatga aattctttaa gtaaactagt
     1981 taacataagt ttagtaagat tcctattctg tgaataatac gtatcagatt tatcttttat
     2041 tcttactaag aaaataacta ttaagggagt tgatttgaat aatggttagc aagaataata
     2101 agagagtatt tctagaaaaa acaaagaaac gagtattgaa gtacagtatc aaaaaattaa
     2161 gtgttggggt tgcatctgtt ttggttggtg ttggtcttgt cttgggaaca actgaactag
     2221 ttcaagcaca agatgaaatt tctccaagca ccccacttga aacagcgata agctccgtac
     2281 aagtaggtga caaagtagct tcggggaata cttttcagga gaatcctggt tatacgaaaa
     2341 attataactt ttcggattta caatttagtc ctcaagaact aactggagac acactaaaag
     2401 gcaatacaat tggatttgaa gtttacggaa aacataatat tgcagcatca actaaaaact
     2461 gggaaatccg tcttcaatta gatgaacgat tagctaaata tgtagaaaaa attcaggtcg
     2521 atccaaagaa aggaattgga tcttctagac ggacctttgt aagaattaat gattcgctgg
     2581 gtagacctac aaatatttgg aaggtcaatt atattcgtgc aagtgatgga ttatttgctg
     2641 gggctgaaac aactgataca caaactgctc ctaatggggt aattacgttt gaaaagagct
     2701 tagatgaaat ttttaaagag ataggcatag acaatctcaa gaccgatcgt ttaatgtatc
     2761 gaatctactt ggtaagtcat caagacgatg ataaaattgt accaggtatt gatagtacgg
     2821 gctacttttt aacagattca gatgatttct ataatagctt agatgtatct gaaaataatc
     2881 ctgaccagtt taaacatgga tcagtaagtg caaaatatga ggaacctaat actcaaacaa
     2941 aagatggttc aggatccaca ggtgctaatg gagctataat cttagatcat aagttaacga
     3001 aaaattataa tttctcatat tcagcttccg caaagggcac gccttggtat gctaattaca
     3061 aaattgatga acgattagtt ccatatgtag cgggtataca gatgcacatg gttcaggctg
     3121 acaaagtaac atatgatgtt tcttttgaat cagggaaaaa agtggctgat ttagcaattg
     3181 aacggcgcaa agatcatgaa aattatggtg taggttcaat tactgataat gatcttacca
     3241 aacttattga ttttgctaat gcaagtccac gtccagtcgt tattcgatat gttttacaat
     3301 tgactaagcc attagatgaa atcttagaag atatgaaagc aacagcacaa gttgaagaaa
     3361 ataaaccatt tggtgaagat ttcatctttg attcttggtt gtcggatacg aataaaaaat
     3421 taatccagaa cacttatgga acaggttatt attatttgca agatattgat ggtgatggaa
     3481 accctgacga taaagaagag agcggagaca cgaatccata tatcgggaaa cctgaattag
     3541 aagaagtata tgatgttgac acaacagtta aggggaaagt attcatccac gagttagcgg
     3601 gaacaggtca caaagcccaa cttgttgata aagaaggtac tgtattagca gaaaaaacta
     3661 tcgctccaaa tgaaaaagat ggggctccaa tttcagatac tgtagaattt gaatttacgg
     3721 gtgtagattc aagtaaatta atcgcgaaag atgaattaaa aatccaaatc gtttctccag
     3781 gttttgataa accagaagaa ggttcaaccg ttattaagga atcaccaaaa gcggttgata
     3841 aacaaaccgt ggtagttgga tttaaaccag atgctaaaga atcaattcgg aataataaaa
     3901 acttacctga agatgcagag tattcatgga aaacagagcc tgatacttct aacgttactg
     3961 atagtacgaa aggtattgta actgttaaga tcggaaatcg aactttcgac gtggatgtag
     4021 agtttgctgt aaaagcttct caagctatgg aaaatgatgc aacatacgta cctataacaa
     4081 caactccaga aacgacagtt caaagtggaa aacctacatt tgataaacca gatgttcctt
     4141 tagctaaaga tgccttttca attttagatg tctataataa ggacttcggt aatgcaagtg
     4201 ttgatgcaaa tactggtatt gttacattca ctccagctaa aggtgtagga gaatcggagc
     4261 cgattactgg aacaattcct attaaaattg tttaccaaga tggttctgta ggcacgaccg
     4321 atttagcagt aactgtaagt aaagatattt atgaaaatcc aggagaaaac attcctgcag
     4381 gc
//
LOCUS       DANKMN010000128         4352 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000128 DANKMN010000000
VERSION     DANKMN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(103..645)
                     /locus_tag="PW224_002802"
     CDS             complement(103..645)
                     /locus_tag="PW224_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909420.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(958..1509)
                     /locus_tag="PW224_002803"
     CDS             complement(958..1509)
                     /locus_tag="PW224_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2909421.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            complement(1802..3733)
                     /locus_tag="PW224_002804"
                     /pseudo
     CDS             complement(1802..3733)
                     /locus_tag="PW224_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            4032..4346
                     /locus_tag="PW224_002805"
     CDS             4032..4346
                     /locus_tag="PW224_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2909422.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
BASE COUNT         1307 a          863 c          726 g         1456 t
ORIGIN      
        1 aataacccac atgaatgatt tgaaatcgtc ttaaaatgct ttattttata acccaaaata
       61 gaataattac ttcaaaatga cgtcgcatat cactattaat gattaatcga tgacttccat
      121 aatcatttta agttccccgg atgttaaatc ctcgctaaaa atgccagtaa ctttttgtcc
      181 aggtccaatt tcctttgatg ataggtttaa caaagagata gggatataga ttaatccgtt
      241 ttgtttcaaa gacagagcca atattttttt ccttgttcga atagctgcat catagcgaag
      301 agaagagtaa acacctctgc caacaataga aatatgcttc cccaataata aatcaaggct
      361 acctattact tgtaatttct gaaaggtcgt cccatccgtt ttgatttgaa tatcctgtaa
      421 atttattttc ctgtctaagg catcttgaaa aaacaagtgt gtgcctctgc gataatcaat
      481 gccacagagg tgcataaaat tagttgtcga aaaaataatc gctaccgttg agacttctcc
      541 gacaaaggat acttttttat ctacaaaatg ttcttgtaag agatgagcag ccttataaat
      601 cgcgtttaac tgtctggtaa tttgggttgt ctcttttata gacatgcgtt tgtattcttt
      661 ttttgccgcg tgcgaagaat gctccttttt cccaataaaa aaagctgctc aaaagagcag
      721 ccttttgtac aggtttagtt ttggtgcttg ccacccgccg gacccttacg ttccggattt
      781 gtatcgagtt tagttttggt gctcgccacc cgccggaccc ttatgttccg gatttatatc
      841 aagttgacta tgagcctgct ctcatttgta tatataatat accaaaaaaa ggttgatttg
      901 tccaatactg tgggtgatta taaccgtctt actgtgctta tctattatat aatacaatca
      961 aaaacctaac cgaaaattta ataacgtttc actgatttca tcttcattaa ttccgatgta
     1021 gcgtttggta atcttctcgc tgctgtgacc aaagatttcc attaaggtgg ccacgtcttt
     1081 ggttttcttg taatagtggt aaccaaaggt ttttcgtagg gtgtgggtgc cgatatcgtc
     1141 ccttcctaac agcttcgcga ccttttgaaa catctggtag accgtattga cttctaaatg
     1201 gccgccctta gtactgggaa acaagtaagc ctccggatct aagggtgcgg tatagccgtg
     1261 aatcaagtcc tgtaagctgc ttaagtataa aatgcgtgtt ttccctgtct tttgctccac
     1321 gatgcgcggg ttttttgagg taatcacgtc ttttttcttg agtttaacga tgtcggacat
     1381 gcgcagaccg ctgttaattc caattagaaa caagaaaacg tcacgttccg catttttatt
     1441 ccgtcgcagg caaaataaaa aatcattaat ctcctgttgc gaccgtagtg gctgaacatt
     1501 atagcccatg acaatccctc ctttttagtc tgttcgaata catgattata gttattacaa
     1561 cacgaaatcg tgtgtttagg gggagtaaaa aaaaagaacc ctataaggat aggattcttg
     1621 atacatgatt ttatccaatt tcatgtatct tatattcaac ccgcgccaca agagtatggt
     1681 tgatatatca ttttgacttt gaacatctag ataaatataa acaagtaata gagataaaaa
     1741 atgtagtgta atccttaatt atctctatta ctcgattaat ttgaagttgt gcaatcattc
     1801 tttagactgc ctttagcaat cgaagtccat tcaaaataac aaccaaagta cttccttcat
     1861 gaataatgac actgattgca atatccgtta agcctaagaa actaacgaca attaaaaaag
     1921 caacaacggc cattgaaaaa ataatattct gtcaaataac acggttcatt ttcgaagaaa
     1981 tattatgaga ttgtaccaat ttagataaat tgttttgcat taaaactaaa tcagatacct
     2041 ctactgccac atcagtccca tctcccatag cgattcccac atctgcatta acaagagcag
     2101 gagcatcatt tacaccgtct ccaaccatgg cggtcacgcc gtatttttct ttttgttcgt
     2161 ctataattct ggatttgtct tccggcatga cattcgcaat cacttcatct attcctaatt
     2221 gtttagcaac cgcttttccc gtcatttctg agtcaccagt aattaatgtg gtgtgtatac
     2281 cttgtttcct aaaatattca atggtttctt tagcatattc actcggaacg tccataaggg
     2341 caataaggcc tataacctct tcatctactg ctacgtatac gactgtctta ccttctgacg
     2401 cccattcatt atttaaacga ctatattcat ctgaaacatc atcaaacgaa gtcggttttc
     2461 ctatacgata atttctccct ttgtaatctc ccgtcaatcc tttaccaatt tggttttcta
     2521 cttcaatagt tagtttgttt ttttgctcaa actttcttag aatagcatct gctaaggggt
     2581 gattggattc tttttcaaga gctactacga tatcaatcat gttttcttcg ttcacggaat
     2641 cagcaaaata ataattggtt acttcaggtt ttcctttggt caaggtgccc gtcttatcaa
     2701 atgcaattgc tttaatatcg gctaattgag acaggtaaga actacctttt gaaaggactc
     2761 cttttttggc taaattagaa gtcgtcgata gcgttgccga gacagtagct gctgctaaag
     2821 cacacggtga agctgcaact aaaaggacca atcctctata gacactttgt gaccatgtcc
     2881 aatcaagtag aaaaggagcc aataacatga atagaggaat ggcaattaaa acaactgtga
     2941 catatttcgg ttcaaatttt tggataatac tcgcagcttt tgtttggtta tcttggttct
     3001 gattcactaa ttgtaaaatt tttgaaaata ctgtatcttt gttttctttg gtgactttca
     3061 tcgtgaaggt acctgttcca ttgattgtac tcccgaagac gccatctcct ttggatttct
     3121 ctttcgggat actttctcca gtaatagacg actcatcaat ggatgtagat ccagacaaaa
     3181 tgacgccatc aataggtact tgatcgccat tcaacacttg aagttgatct cccactttta
     3241 attcactgac atccacattt tttgtactac catcaggcag gattaaccgg gcagtcgttg
     3301 gattcatttc gagtaatttc gttatttctc gtttgcttct tccttctgca taatcttcca
     3361 aaaaatgtgc gccagaaaaa ataagaatca ataatgttcc ttcccaaaaa ttccccatca
     3421 aagaagctcc aattgccgct aatcccatta aaatatgaga attaggtatg aacttttttt
     3481 gtgcttttga attctcaatt gtttctccaa ggccttcaag aagaataacg tgatacccgg
     3541 cactgattgt agcgattgag aacaaaacat tttgtaccaa ctgataatct tcattcaaaa
     3601 atagagcaat cactgctaat gctaaaccaa caaagtacaa aacgactggc attttcccat
     3661 gatcatggtc gtgatcgtgg ttgtgattat gattatgctt ttcttgagac tgttgttctt
     3721 ggatagtttc cacttctatt gcctccctat ataatttata attcctttgt tttccatcat
     3781 ttacttcttg aatcacataa atttctgcta tttctgactc ctttcgttta ttccaaaagt
     3841 aaatcgtaat gaatccacct tacatatgac taattaatca tatgtttgta tactcatttt
     3901 atatcgctat atacattttg tcaagataaa gagaacgcca cttctctttt ataaacaaaa
     3961 gattagcatt gtttcgctca tatggtaata tagatatgaa cggaaggtca tatgaaagga
     4021 ataaaaacac tatggatata aaaacaactt ctccaatcga taaaggatct attctgtcaa
     4081 taagcaaact atttaaggtg attagtgatc ctacacgatt gtcggttctc tttcttttac
     4141 agaaaaaaga gtgtagtgtt ggaaatatcg ctatttctct ggacatggaa cagtccgcta
     4201 tttctcatca attaaaaacg ttgaaagacg cacgcttagt gaaatcaaga agagaaggaa
     4261 aaagtatgct ttacagttta gatgaccttc atgtttttag tattctcgaa caagtcttaa
     4321 ctcatgttaa tgaattagaa aaataaaacc aa
//
LOCUS       DANKMN010000129         4265 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000129 DANKMN010000000
VERSION     DANKMN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            635..1021
                     /locus_tag="PW224_002806"
     CDS             635..1021
                     /locus_tag="PW224_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2909423.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1119..1310
                     /locus_tag="PW224_002807"
     CDS             1119..1310
                     /locus_tag="PW224_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2909424.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1824..2156
                     /locus_tag="PW224_002808"
     CDS             1824..2156
                     /locus_tag="PW224_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2909425.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2066..2269
                     /locus_tag="PW224_002809"
     CDS             2066..2269
                     /locus_tag="PW224_002809"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2909426.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            2453..2608
                     /locus_tag="PW224_002810"
     CDS             2453..2608
                     /locus_tag="PW224_002810"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2909427.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2884..4224
                     /locus_tag="PW224_002811"
     CDS             2884..4224
                     /locus_tag="PW224_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2909428.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         1483 a          786 c          728 g         1268 t
ORIGIN      
        1 actattcctc tgtcaaaata agctaactta cttcattata aaatatccac ttatcgcttt
       61 catgtatatt ccaccgtaaa aagtacattt tatcaccgag aactcatcat ttttcatcat
      121 gatgaatact atttacatat atctagttca aagacaataa ttattatgat ccgttattat
      181 ctaagcacta tacagataat aggaattttt cgtttctaaa tactccaatt tgggaatcca
      241 ataaaaattt tcgttcaaat acgtgtctat tcgaactctt ttctagttgt taattatgaa
      301 gggggaagaa ataaaaaata caatctatac acgaataaga atattatcca aaaggaggtt
      361 ttagaaaaga tttattatac gtaaaaagct caagacattt tcttagccat aaggacatgt
      421 ttgatattta agaagatata tgaattttct tttctaagta taaagataac tatccattat
      481 caactaaaaa aacatttttg aaaataaaca tcagcagtcc tatcaatgca tgtgagctta
      541 tagaagttat tcgcagcgca gcataattgc attagcttct tttcgtgttt tttgcctctt
      601 ttccctttaa atcttaatgg aggaggtgtg caatatgtca gataacgacg aacgcgtaaa
      661 tgcccagttg attcgattct ttgaaaaggt cattttgaac accgctaata cttattataa
      721 aaaacaagaa gaaattcaaa aacatgaaca acttgatcaa ctcccaccat atttgtttac
      781 taaagatact ataaaattta aacaaccgat taatgtttta aatgtgactc tccttgttga
      841 agatataaaa ttagcaaaaa tccttccttt gttgaaagaa cctgaacaaa tttttcttat
      901 cgaaaaattt atctttaata aaactgataa agaaattgga aaggttctgg gtattacaag
      961 acagggtgcg acaaatttaa aacatcgact ttacaaaaaa attcaaaaaa tgctggttta
     1021 atatctgcat aataactaat tttgtatgct tctttgctcg tcctcctcct tccccaaaaa
     1081 ataaatagat tataagttca tgggaagaag gtaaatttat gaatacttta aaagaagatt
     1141 tcattttagc aaagaatgga aatgaagaag cagttgtggc cattctcaaa agatttagcg
     1201 ctttggtcca taagcaatcc tggagaactg gaaaatatga tcaagattgt tatcaagaat
     1261 gtatgcttgc aatatatcta gctatctcga aattcgaaat taaagaataa ctcaactaag
     1321 ggagtctgtg acataagtaa aaagatagtt tcaaatttcg aacaataaat ttgaagccat
     1381 cttctttata attataaagt atcgatttta cagcgttcta taatgattca tagaatctaa
     1441 aacgaatgat tttaattatt aacgtttttt aattcgtttt tagcttaaaa ataacttttg
     1501 tcacagtctc actcctaaaa ttactttatt tacgtacgaa gtgggcagga ggtcgaatcc
     1561 agaactgggc catggtactt caacgaggac ttctcctcta gaattgaaaa gcatgcgatt
     1621 taccttccgt aacgacggta gatttattca actatgtgtc caatcaaaat ttgtctgatt
     1681 tcaaaaatag tcaagagaat tttggttgtt aaaaactctt atctatcaac ttccacagtt
     1741 tagttgacaa accctgatac taagttgggc tatgatacac ttttcttgac tatcagttac
     1801 gttaccggaa ggaatgaact ctattgaaaa aaacatatga tcgtaaattt aaactaaaga
     1861 tttcacagga tatccttgag aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata
     1921 tctctcgtcc aacaatttcc cgatgggttt cagaatatcg tcgatacggg aaaaatgctt
     1981 ttgctggaca aggaaaaaga ttacctgata aagcagattt ctacattctt gaacaggaaa
     2041 ataaacgact aaaagaggaa gacgagtgtt ctacgaacac aaagatcgat acggatctcc
     2101 agggatcact caaaaacttt acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt
     2161 tcttatgcgt gaaatgaatt tatgcacaag aggatttcat cgacgcaaat cttctcatgg
     2221 tagagaaaaa acaatcaaag aaatggtaaa aaaaatgttg aatcgataat ttgaacagaa
     2281 ccaaattgat gctgtttggg tgacggatat tacttacatt ccatgtaatg acggtcgcct
     2341 ctatttgtcc acctatattg acttagcaac tcgtattcta cgttactata tggtggattc
     2401 acacatgaaa aaagagattg tcgtccgtcc attacaaatt tattaaggag aattgcctac
     2461 ggtaattcat tccgatagag gtagccagca tcggtctttt atttatcaag agctgttgac
     2521 cgaccaccac atcacacatt caatgagcca aactgggact cctgttgaca atgcagtaat
     2581 tgaagcgttt catcgatcaa tcaaatgaga attaatcgac tccaacaaat ataaaagtaa
     2641 aatagagatg aaggtgttaa tccaagatta tttagaagac tactacccaa acaaaagaat
     2701 ccatacaaaa tttatgatga ctccttgaca atttgaagcg aagaaaataa gggatctata
     2761 atatctagtg ttggtgacgc cttctgttgc caatactttt tcttttttct aataaacgca
     2821 aacagacacc ttgaaatcct ttagaataaa gtcgacgaaa accattcaaa ggagtgtttc
     2881 aagatgtcct attcagagtt taccaaagag ttactagata ttctagacct aaatcttacc
     2941 tttcatgaag atgcctttcg caaagagcgc attaatgacg aaacatgttt tgtttttgat
     3001 ggcacattga cctatcaacc agaggaatgc ttccattgcc actatcaaaa taagcaaacg
     3061 atcattaaat ggggctggaa aaaagtatcc atcttattga atgatgtcag taactacaag
     3121 accattgtgc ggattaacaa acagcgcttc aaatgtaaac actgtggaaa gactttttta
     3181 gcagaagatt cagttagcga tcgtcgctgc tccattgccc gcagagtcaa acaagccatt
     3241 cttgagctat taagtgagcc tatttctatg tctttgattg cacgtatgaa gcacatttct
     3301 ccgaccactg tcattcgaat acttcgcagc cttcggccta aaaccgtttc tttgaaccag
     3361 ccgttaccag aagtggtgtg tttcgatgaa ttcaaatcgg tgaaaaacgt ctcgggagcc
     3421 atgagctttg tcatgatgga cggcaagacc catcaactga ttgatattgt ggaaaatcgg
     3481 caactcaatc ctttacgcga ctatttcttg cgctacccta gaaaagtccg tgaacaagta
     3541 cgtctagtgg tttcagattt ctatacaccc tatcgcacat tggtgaaaga gtgttttccg
     3601 aatgcccgaa tcgtcgccga tcgctttcat attagccaac acatcggtcg ggcctttacc
     3661 aaccaccgaa ttcaagtaat gaagaccttt aaaaaagggg atcgtcggtc gaagcactta
     3721 aagaaatact ggagactact tcaaaagaat gcgtgggaat tgaatggtca gcatcgctac
     3781 tggcgtccgt catttcgaga tcatctgaca gaagcggaga tagtcgatcg gctattatat
     3841 tatgatgact ccttaaagcg aggctacgaa gtctaccagg tttttctttc cgctatcaga
     3901 agacaagatg ttcctgaatt cgtcgcgctc ctaaaagaag actacaagga gttgccagag
     3961 cactatcaac cagtgttcac gaccttcaag aaatatcgaa cagaaatcaa acgagcgttg
     4021 cgagttccct attccaacgg ccctatagaa tgtttgaaca accatatcaa agtactaaaa
     4081 cgaattgcct atggctttag aaactttcaa aactaccgag aacgaatctt tttatatcga
     4141 ggaaaatatt tcaagaaaac taagaacact acccaactga ctaaagccag aacaacaaca
     4201 cggctagaca agatagctgt ctagccgtgt gagtatctcg attattctat tgaatttctt
     4261 cacca
//
LOCUS       DANKMN010000130         4153 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000130 DANKMN010000000
VERSION     DANKMN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..106
                     /locus_tag="PW224_002812"
     CDS             <1..106
                     /locus_tag="PW224_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2909429.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="PW224_002813"
     CDS             complement(359..508)
                     /locus_tag="PW224_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909430.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="PW224_002814"
     CDS             970..1182
                     /locus_tag="PW224_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909431.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="PW224_002815"
     CDS             complement(1460..3259)
                     /locus_tag="PW224_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2909432.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="PW224_002816"
     CDS             complement(3383..3979)
                     /locus_tag="PW224_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2909433.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DANKMN010000131         4067 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000131 DANKMN010000000
VERSION     DANKMN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            86..1435
                     /locus_tag="PW224_002817"
     CDS             86..1435
                     /locus_tag="PW224_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2909434.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="PW224_002818"
     CDS             complement(1563..2153)
                     /locus_tag="PW224_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2909435.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="PW224_002819"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="PW224_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2909436.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1173 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaa
//
LOCUS       DANKMN010000132         3975 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000132 DANKMN010000000
VERSION     DANKMN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(103..354)
                     /locus_tag="PW224_002820"
     CDS             complement(103..354)
                     /locus_tag="PW224_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2909437.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            complement(368..559)
                     /locus_tag="PW224_002821"
     CDS             complement(368..559)
                     /locus_tag="PW224_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909438.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            852..1172
                     /locus_tag="PW224_002822"
     CDS             852..1172
                     /locus_tag="PW224_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2909439.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1190..1612
                     /locus_tag="PW224_002823"
     CDS             1190..1612
                     /locus_tag="PW224_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2909440.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            1695..2330
                     /locus_tag="PW224_002824"
     CDS             1695..2330
                     /locus_tag="PW224_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL2909441.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            2448..3659
                     /locus_tag="PW224_002825"
     CDS             2448..3659
                     /locus_tag="PW224_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2909442.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
BASE COUNT         1461 a          644 c          696 g         1174 t
ORIGIN      
        1 caagccctat aaaaatccaa accatgtaca caatcgttcc tatcaacgct tgtctatttc
       61 cccaaagccc taaagcgtag atgattagcg gtgcgctgaa tactagtgct ctgttgaatt
      121 tgcccatgtt tttcctcctt ttagatactg atacaaagcg atactactaa acctccactc
      181 tctgccaact ttcgctgctg gaatctttcc agattcggca tctttagtta gcgtgcgtgt
      241 cgtggttttc aaatattccg cagcttgctt tgtatcccac acttcatttg caatctcaga
      301 ttctgccaaa gaagctttga gggctgagag gttaactaaa gctagttgtt ccattgatta
      361 attcctccta tcgaattctg taatcacgga tgatttctaa gatagtttta ttagcttttg
      421 gtccactcca atggccatca ataatttgct gcattcgaac acgtgtgtat ccatatgcag
      481 tagccaaatc ttccatagtt actccgtttt ctcgcataaa cttcttgatt gcagcacggc
      541 cgttatctag atttgacatc tattcactcc ccttacatat agatttgtaa gtcaaaatga
      601 tagaaaaaac gtataaacta ttgactaata gtatacaatc gtatactata taaacatagt
      661 taaataagcc tacaacaaac cctttattat gcaatcggtc gccaaactta atgctataag
      721 gtgtattttt agtttgcttt ttttctatca aattaactta caaacaaata ataatacaaa
      781 cttatactta tgtcaacagt ataatttaca ttttgtgtac ttttatttgt ttaggattgg
      841 agaacattat tatgacactg tttgaaagga taaaatcatt agctagccaa agagataaaa
      901 gcatgaaaga agtcgcttta gaattaggat ttagcgaaaa tcttttctat cgatggaaaa
      961 caacagaacc caaagcaaga gatttgcaaa aggtagctga ctatttcaat gtctctgtag
     1021 actatcttct aggtagagaa gaaaaagaaa cgcctaaaca tgtggattta tcagaagatg
     1081 atactgtatt ttcttttgat ggaaaagaaa tatctaagga gacaatgcgt aaagcgattg
     1141 caattgctaa agctttagag gaaaatgaat agttggagtg atgggttgta tgtatttaaa
     1201 gttgaaagaa atgctgagtg agtataattt aaagttaatc tatatggaaa tggaagaacc
     1261 aggtttttat tatccaaaac caagaatagt atttttgaat gaaaaactac acgaagacag
     1321 ttctgaagct tttcatttag cccatgagct cggtcatttc attgcttcac attttgaata
     1381 ttcagtactg tacgataact ctacaacttt tcattcaaaa ttcgaagctg aagctgatag
     1441 aatcgcaatt atgattttac taaatatctt tattgagaac gaattgactg atgaatctca
     1501 gttcaaatta gaaaatttca tggaattcta ctctatcaat aataagttaa gaacagaatg
     1561 ttttaatgtt tgccagtcat atttcaagaa aaaatactct tatgcacagt aaaaaaagcc
     1621 cgtgctgcaa cacggactca tacctcattt ctgagatcac aaatatatta taacaagaag
     1681 tgagggatat ttaaatggca aaaaaagtta tgggtcaaga tgggaaaatg tataaggtta
     1741 aaaaaccgtt ttataaacgg gtatggtttt gggtattaat agtacttttg ttttttggta
     1801 ttggtggtgc tctaagtggc ggtgaaaaca aagattcagc tactgcaaca tcaacaagta
     1861 ctaataaaga aactacaaaa gaaactacaa caaaagaaga aaagacttat aaaatcggtg
     1921 atgacgttgc tgtagggaaa atggaataca aagtaaactc cgttgaagta gtaaaacaag
     1981 ttggcccatc tgtacttcct actaacgcaa aagacacttt cttagtagtt gacttatctg
     2041 taaaaaatgc aggtgataag gctgtgactg ttgatagctc attcttcaaa ttaaaagcag
     2101 atggaaaaac tttcgaagct gattcggctg catctatgtc ggcaaatcaa gatgaaaatg
     2161 gtaatatcac taactctttc tttatggaaa gcttaaatcc tgatatgcaa caaactggta
     2221 aaatagtttt tgatatctct gaagcccaag caaatgcaca aaataatgta ttgcaagcac
     2281 aaacaggtta ctttggaaca gagacagttt caattgctct tcataactaa ttagtataaa
     2341 aaacacgccc caccgaccaa agcgagcgtg ttctaagaaa aaacaaacct acacaatagg
     2401 cttctttata gtacctattg tatcagagaa agagagctga ttcaattatg tcagaagtaa
     2461 caggatactt agaacaagta gacaatgaaa catatagact aagagctgta ttagggtaca
     2521 aacctgatgg ctcggcaaaa agaaaaagca aaactataaa agcgaatagt aaaagagcgg
     2581 catataaaga attgaacatc tggttagagc aattcgaagg aatgaccgat gattcgttgg
     2641 atttattcaa tataactttt ggtgaatttt atagaaaaat atggctttca gaggccgaaa
     2701 aaaatttaga gccaaagtct tatcataact ataagcgaat gatcgagaat agatttcttg
     2761 ataaatttga ttttatccct ctaatcgata taaaaccata catgataaag aaaattgttg
     2821 ttaatgctca aagaatcaat acaaaagatc ctggaagaaa ctctgataaa cctttatcaa
     2881 gaaatacaaa gcttcgtatg ttatacgccg ttaacaactt atttttgatg gctaaaaatg
     2941 agtacggagc aattaaagaa aatcctgtgg aaaatgtaaa aataccaaaa gaaaaaggcg
     3001 taaaaaagaa tatcgaagaa ccttattcgg aagaagaaat tcatgcaatg ctaaaagcag
     3061 catttgagga aagcatagaa attaaaactt taattgttct agcatttatt acaggagcaa
     3121 gacaaggaga aattgcagct ttagaagaaa aagatattga ctttgacaaa caagaaataa
     3181 gatttcatca gcggatttca gaagtggatg gaaaatcaga tattcggttg ctgccaggtt
     3241 tgaaaaatga tgatgatgaa aaaatagtaa ccggtcctgc ttatctattt gatatgttag
     3301 atgaattgat caaagagaat aaaaaaattc gttggaaatt aaatataaag aaattaagac
     3361 actattttat atttgataca aaacaagacg gaactttacc acgtggaagt tatctgtata
     3421 agaagttcaa acgattcacc aaacgtcaca atttgcgtca cattcgtttt cacgacatta
     3481 gacatacatc agctacctat ttgcttagcg atcctaatat gactccaaaa gagcttcaaa
     3541 aacgattagg acaccgagat tttaatacta caatgaatgt ttatggccat gtcttacgca
     3601 aagaaaaaga tacagcaact acagcgtttg aaaaactatt aaaaaaagat ataaaataac
     3661 actaaaaaat tatattttcg ggtataaact cgggtaggat atataaaaac ttgggataat
     3721 ttgcggtata taagcattta aaaatgctgt aaattcgctt atcttcattt aaaataccat
     3781 taaataacga actcttaatc agcgggtcgc gggttcgagc ccctcacggc ccattgggtg
     3841 ccaaacccac ggaacaggta aatgttggtg cgaaagcatt tcgaaaacat ttacgctgtt
     3901 ctttttttat tttcgccgtt tgtataatta agcgagacaa ataaaaaagc catttatgtt
     3961 acactcaaat tgtaa
//
LOCUS       DANKMN010000133         3850 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000133 DANKMN010000000
VERSION     DANKMN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(3..1157)
                     /locus_tag="PW224_002826"
     CDS             complement(3..1157)
                     /locus_tag="PW224_002826"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL2909443.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1342..3783
                     /locus_tag="PW224_002827"
     CDS             1342..3783
                     /locus_tag="PW224_002827"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL2909444.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1232 a          721 c          840 g         1057 t
ORIGIN      
        1 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
       61 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
      121 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
      181 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
      241 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
      301 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
      361 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
      421 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
      481 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
      541 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
      601 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
      661 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
      721 gccaagctgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
      781 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
      841 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
      901 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
      961 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     1021 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     1081 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     1141 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     1201 gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt
     1261 aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
     1321 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
     1381 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
     1441 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt
     1501 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
     1561 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
     1621 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
     1681 ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa
     1741 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
     1801 catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag
     1861 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
     1921 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
     1981 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
     2041 ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt
     2101 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
     2161 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
     2221 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     2281 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     2341 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     2401 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     2461 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     2521 gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata
     2581 gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa
     2641 gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc
     2701 gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca
     2761 tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca
     2821 cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg
     2881 atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct
     2941 tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt
     3001 ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac
     3061 atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa
     3121 gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt
     3181 gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa
     3241 atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa
     3301 gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt
     3361 agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg
     3421 cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta
     3481 ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat
     3541 tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct
     3601 ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca
     3661 ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt
     3721 gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa
     3781 taaaaaagca atatctttag aagaataaag caagatttac agttatttta tttactgcag
     3841 atcttgcttt
//
LOCUS       DANKMN010000134         3791 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000134 DANKMN010000000
VERSION     DANKMN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(95..592)
                     /locus_tag="PW224_002828"
     CDS             complement(95..592)
                     /locus_tag="PW224_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL2909445.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(722..1432)
                     /gene="budA"
                     /locus_tag="PW224_002829"
     CDS             complement(722..1432)
                     /gene="budA"
                     /locus_tag="PW224_002829"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL2909446.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1445..3109)
                     /gene="alsS"
                     /locus_tag="PW224_002830"
     CDS             complement(1445..3109)
                     /gene="alsS"
                     /locus_tag="PW224_002830"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL2909447.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3314..3781)
                     /locus_tag="PW224_002831"
     CDS             complement(3314..3781)
                     /locus_tag="PW224_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909448.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1127 a          819 c          602 g         1243 t
ORIGIN      
        1 aaaaaaataa aaaaaccgaa aaacagaaat gcccactgtc tttccggctc tttttattct
       61 tgatttctct tcttttttgt ttcaatagac gattttagtt attaataaat gaaaaagcat
      121 ttatattaaa atttgctaat aaaagatcta catatttttc tagaaatgct tgatcgattt
      181 catcataccc tttcgttact ccgcaatcga tatctaaaac accgattaaa ttaccttctt
      241 ttaccattgg cactacgatt tctgattgtg cggctgaatc gcaggaaatg taatttgcat
      301 gggttttgac atcatcaacg atcaatgttt cttgtttttc agctgattct ccacaaactc
      361 cttttcccat cttgatacgt gtgcaagaaa ctcgcccttg gaacggtcct aaaatcaatt
      421 cattaccatc gaataaataa tatcctgcaa agacagtctc aggcaaagtc tcattcaaca
      481 atgcagatga atttgctaaa tttgctaacc agttatcttc tgcttctaat aatccttgtt
      541 gctgtaataa taaaatttcg tatgcatctt ttttctgttt ttctgtccac atgataaaaa
      601 ccgctccttt ttgatctatg gagaagatta tagctcagtt tttttgtaaa cgctaggttt
      661 cctcctcaaa aaaacgagat acgtcaggtt aatcaccttg aacgcatctc gctgtctttt
      721 tctattctgc ttgttggatt tctgctgcta aatcagcata atcaatcgtt tcgtgaacaa
      781 aagtcggatc atcaactgga aaatgctgta caagggacga gatgcggcta atctcaactt
      841 ttccttcagc cacttcaaaa tccattacat gtccgccaaa tgtatgtgta tcatcgataa
      901 aatgcaagtg aaatcccgct gcagcaactc cttggaataa ttcaggcgta aagaacccga
      961 ctaatgttcc ttggacattg ctttgttgga attctggttg agactctgaa atttttgcta
     1021 gtcttctgta aggtttttct tgcttcggca tgatacggat gtgcatattt ttgaataatc
     1081 cagttatctt cactgccaaa aataggtttt ccccggcttc tcttactaat atgtcttttt
     1141 tcaattgttc tccatctgca gtttttgaga cagaaaaact agtatctggc gtaaaatctg
     1201 taattgctgc atagggggta agttcacttc catctaattg ggcaactgag ccatctgatc
     1261 ttccttgata agctacgcca tctaaaaaga tgacttcacc atcaagccca tgcaatgttc
     1321 ccaatcccag agttcctttt tctaatatat gagcaatcgt ttctgttcca tccatcaagc
     1381 cagccatcaa agctcctaat gtcccgtgct ggtataagat tttttcagtc atgtcgacac
     1441 ttccttaata gaattgatct ggtaagattg tttctcccaa tttctcatta tcacggtaat
     1501 caatcggaat atcaataatt acgggtcctt ctgtcgcaaa tccttcttct aaagcttttt
     1561 ctaattcctc tacacttgta gctcggattc ctttagcgcc aaaagcttct gcatatttca
     1621 caaaatcaac tggaccaaag tctacacctg acgcccgctg atatttcatt ttttcttgga
     1681 actctaccat attgtagtgt ccatcattcc aaatcaaatg aacgatattc aattttttac
     1741 gtaccgccgt ttctaaatcc tgggcagaga ataaaaatcc cccatcgccg gaaactgata
     1801 cgatttgtgt atttggacgc accaatgcag cagaaatcgc ccatggcaat gcaacaccaa
     1861 gcgtttgcat accattactg aataacaaat gacgaggttc ataacttcta aagtggcgtg
     1921 ccatccaaat ataatgacta cctacatcta cggtaacagt catatcatct gttacttttg
     1981 actgaagcgt gttaataact tctaaaggat gcaagatacc tgcttctcct tttgattgaa
     2041 caatgtcccg ctccgttaat tctgcttgaa gagatgctaa atattctttt gcatcttctg
     2101 acacttgttg aggctctaat gaacctgtca ataaatcaag agtggctgag atatcaccga
     2161 tcaattctct ttcaggctgc ataaaaggat cgatttcagc tggcgcttca tcgatcacaa
     2221 tgatccgcgc atctttttcc gcattccagt ttcttgcttc atattcgatt ggatcatagc
     2281 caatagcaat aactagatca cttcgtttca aaagcatatc tcctggttgg tttctaaata
     2341 aacccacacg accaaagaaa tgcgcttcta attcacgtga gatcactcca gctgcttgga
     2401 atgtctctac aacaggcaat gctgtctttc caactaattg cctgatggct aacgtttctt
     2461 tttcacttga tcctcgcata cctactaata gtaccggtag ttttgcttct ctaatgcgtt
     2521 cagctaggta acggatatca tctgcagaag cagaacctaa tttaggatcg ctcaatggct
     2581 tgatgacatt cccttggaca ggtgcatcca ctacatcttg cgggatactg ataaagctcg
     2641 cgcctttttt tgaactctta gccatacgat aagcattggc aatcacttct gaaattgttt
     2701 ctggatcttg gatttctgca ctgtattttg tgattggctg gaacagtgct gcattatcca
     2761 tgctttgatg ggtcaacttc aatagatcgc ttcgttttac ttgtccggca attgctaaaa
     2821 ctgggtcacc ttctgctgtc gctgttacca aacctgtagc taagttacta gcaccaggac
     2881 cacttgttgc gataactact cctggctctc ctgtaatacg tccgattgct tgtgccataa
     2941 atgcagcatt ttgttcgtga cgagtcacga ttaattcagg accttgatcc tctaattcat
     3001 taaatacacc atcgattttt gcacctggta taccgaatac ataatcaaca tgatgattaa
     3061 tcaaactttc tactacagat ttcgaacctt gtttcatttt ttcgtccatt gaacatcgtc
     3121 ctttttatgc tttattttta attattagtt agaaataatc acaaataatt gatacagttt
     3181 ctaaacctgt ttcattcaaa agtatagcac gttttatttc atttgtttag ccatatgttt
     3241 caaaaaatga aaaagaatga cagtttttac ataaaaactg tcattcttaa ttgaaagttt
     3301 caatttataa agtctacaga tccatagttc tcatcaattt acgattgttg aagtaaatca
     3361 aaccaaatac gatagaagga cctagtagaa tacaccattg gggctttacg aaaaagaaga
     3421 gaaacaaact tgtcaatagt aaaaaatcta gtaaactcac ataccatttt ttcaacgtat
     3481 agtagaacgc tatccgtaga acttgatttg gtttcgacgc tggatttctc acctgaaaat
     3541 accagtagtt gatgctaaag gaaactgata aacaagcaag taaaacaaac aacggaatca
     3601 accattttcc ataagaaaaa cgtataaaaa agaaacaatc aaaaatcatt atcacactaa
     3661 ctatccaagc aaatagccaa taaaaaaatc cctttagcca aaattggcga aacgtcttta
     3721 tgaacaaacg tactggtgaa aaactttttt cttttgctaa ttcttctgtc atacctatca
     3781 tcacgatgca a
//
LOCUS       DANKMN010000135         3677 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000135 DANKMN010000000
VERSION     DANKMN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..444
                     /locus_tag="PW224_002832"
     CDS             <1..444
                     /locus_tag="PW224_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL2909449.1"
                     /translation="KEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRK
                     VEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKS
                     MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            444..1643
                     /locus_tag="PW224_002833"
     CDS             444..1643
                     /locus_tag="PW224_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2909450.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1659..2303
                     /locus_tag="PW224_002834"
     CDS             1659..2303
                     /locus_tag="PW224_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909451.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2314..3120
                     /locus_tag="PW224_002835"
     CDS             2314..3120
                     /locus_tag="PW224_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909452.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3142..3372
                     /locus_tag="PW224_002836"
     CDS             3142..3372
                     /locus_tag="PW224_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909453.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         1360 a          574 c          765 g          978 t
ORIGIN      
        1 aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc
       61 cataatgcga ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga
      121 gaaatgcgta aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg
      181 ccagaaaata tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct
      241 acgaagatct ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt
      301 aaatcaatga cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg
      361 atcaattttg gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta
      421 aaattgtttg atggaggaaa ataatggaaa accaaaatga aagtttaatc aaacagtatg
      481 ttaagcgtcg tgcaaagcgg cgcttatttt tgtgggtgtt tggtacttct gcaggtttga
      541 ttactatatt gatcactgtg tttgtgaccc tttttctaat acttgcggca ggttctattg
      601 ataattctga ttcaagtagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
      661 ccatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgctg
      721 taggtgcagc agtgaagtat aaattattac caagtgtgat cctttcgcaa tacggttatg
      781 aatcagcgtt tggtacgtcg gcgtccgcaa gaaatgatct caattatttt gggattacgt
      841 ggtttgatgg gtgtctgttt cctaaaggaa ctgcgcgtgg aattggtggc attgaaggtg
      901 gctggtacat gaagtttccc aactcaaaag ctgccttctc ctattacggc tttatggtag
      961 ccacacaatc aaattttaat gcttgtgtcg gaaataaaag ccctggcgca agcttattga
     1021 tcttaggacg aggagggtat gccgccgcag gaattactga agacagtgcc tattataaaa
     1081 attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac
     1141 actggagaga aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc
     1201 caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag
     1261 gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc
     1321 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
     1381 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
     1441 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
     1501 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
     1561 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
     1621 ttttgaatag cagcaaaaaa tagatcagga gaaaaaatat ggaaaataaa accttaaaaa
     1681 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
     1741 ttaaagaaga acaagagaca aatacctata aaattgaaga agtggctaaa aacgcgtctg
     1801 ataagctcaa aaaaaattct tctgaggcac aggcgaagat cgacaagtta aatcaaacca
     1861 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
     1921 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
     1981 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
     2041 tcgacaaatt aagtcctgca gacgatgatc accaacatga cgactacgga agttactctt
     2101 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
     2161 ttgtcgtatt tgttgattat acaatcggca atcctcaatt taaagatgtc aaaccacaaa
     2221 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcacag
     2281 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agcaagacaa
     2341 cgttttaaaa aacgaaaaag ttatcttaga tggctgggga tctttatgat tctcggcttt
     2401 ttgattcttg ctagtccgat ttacacacat aaaaaggtag acataccaga tgaagtaagc
     2461 acgaacgcat ttttaaacac gaaggaactg acgatggttt caaaagaaaa aggagcgaac
     2521 cagcaattag tttttgaatt tgcgatcgac gatgaagatc agagcttatt aaaagaacta
     2581 gctaatctta attatcgtgt agaagtaaag acagctaaag gggattatca agcaataaaa
     2641 gccaaagtga tcaaggtgtc tgatgattat tttgtagtga agttagcaca tgtaccagaa
     2701 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
     2761 caagaaccac aagatctgat ttattacgtt cacgaagata aggttaaaga tcaagtgaaa
     2821 gatgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa aaaagaacaa
     2881 aaagcagcac aaaaacaaat ggaatccttc caagcaacca ttgatttgaa tgaagaatta
     2941 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
     3001 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaaat gaaagatcaa
     3061 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
     3121 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
     3181 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
     3241 gaagtgaatt tatctaatac aacaatatct gagttgaaga atggtcgaaa gaatttagat
     3301 actttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
     3361 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
     3421 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
     3481 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
     3541 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
     3601 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
     3661 acaaataacc ttaattt
//
LOCUS       DANKMN010000136         3542 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000136 DANKMN010000000
VERSION     DANKMN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..85)
                     /locus_tag="PW224_002837"
     CDS             complement(<1..85)
                     /locus_tag="PW224_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2909454.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            155..316
                     /locus_tag="PW224_002838"
     CDS             155..316
                     /locus_tag="PW224_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL2909455.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..1612
                     /locus_tag="PW224_002839"
     CDS             335..1612
                     /locus_tag="PW224_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2909456.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="PW224_002840"
     CDS             1622..2278
                     /locus_tag="PW224_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2909457.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..>3542
                     /locus_tag="PW224_002841"
     CDS             2278..>3542
                     /locus_tag="PW224_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2909458.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPK"
BASE COUNT         1431 a          369 c          615 g         1127 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 tt
//
LOCUS       DANKMN010000137         3406 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000137 DANKMN010000000
VERSION     DANKMN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(451..1761)
                     /locus_tag="PW224_002842"
     CDS             complement(451..1761)
                     /locus_tag="PW224_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2909459.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="PW224_002843"
     CDS             complement(1773..3191)
                     /locus_tag="PW224_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2909460.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1066 a          684 c          521 g         1135 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtg
//
LOCUS       DANKMN010000138         3353 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000138 DANKMN010000000
VERSION     DANKMN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            211..477
                     /locus_tag="PW224_002844"
     CDS             211..477
                     /locus_tag="PW224_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2909461.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            504..803
                     /locus_tag="PW224_002845"
     CDS             504..803
                     /locus_tag="PW224_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HDL2909462.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            977..1528
                     /locus_tag="PW224_002846"
     CDS             977..1528
                     /locus_tag="PW224_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDL2909463.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1674..1967
                     /locus_tag="PW224_002847"
     CDS             1674..1967
                     /locus_tag="PW224_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909464.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1960..2256
                     /locus_tag="PW224_002848"
     CDS             1960..2256
                     /locus_tag="PW224_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909465.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2331..3329
                     /locus_tag="PW224_002849"
     CDS             2331..3329
                     /locus_tag="PW224_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2909466.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1261 a          459 c          664 g          969 t
ORIGIN      
        1 gatttagaaa aacttactaa acaaagagat atgaaaaact ttaataattg tgtagttttg
       61 ataattataa ctgaattaac tttatttaca aatatatatt atatccatga agcccaatct
      121 tctttaatag aagattgggc tatttttatg gcaaaggagg gaaaagaaat gtttcaaaat
      181 aaaggataac gttatgtcac aaagggagta atggacagtt tgccggtgga attacaagct
      241 ttatgttgga atctaatcga tcaaaacgtt caaaagcaat ttcctttaga ctatcttcaa
      301 atttttgaat tttctacgga aaaaggaaat caaaagttgg ttcatcgtca agaagaaccg
      361 gaagaaagaa aagagtatct gatttcacct aagcttcgtt tgaaatcagt gtctcaaaaa
      421 atatgggcca ttgattcggg tgagtatcaa acgatgttat taccagaaga atactagtat
      481 ctaaaagaaa ggaacgaaag gacttgttag aaacggctat cgaagtactg gaaaagtgtg
      541 cacagctggt cactgcttct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg
      601 aaattgaaat gggtatgctt cctgaagatg ttcatctacc acgtttagtg atgacacatc
      661 tttatattta ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata
      721 ttaccagcca gcgagaattt gttcgtgggt tactggtaga aggacgattg gtttggagtc
      781 agattggagg aacggatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa
      841 gatatcctta ccgcgattca tcaaattctg aagaaaaaag gagaaagaat atgaaatatt
      901 ttcaactagt attgacactt ataacggcaa tggtaattga agtgcttgaa caaaaaatag
      961 cataggagaa aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg
     1021 agatcctcat catacccatc gctatgtttt caaacgacaa tgggcgatca aaggaaaaga
     1081 agcggaagat aaattagcag tagtcattac tattcgtcca acagatacag aaccatttac
     1141 gaatgatttg agtatgttat taatcgaaaa gaatatccga caattaggat ttactggttt
     1201 tattgctgtg aatctttttt cgtacacaaa agcgaaaagt cctcgggtat ttcttcgagg
     1261 aaatgatgaa caatctttgg aaatattaac gactgttttt acagaaaaga aaatcaatac
     1321 cattattttt gcttgtggtt cgattacagc gacgagtcaa gtggcttatg aacaagcaaa
     1381 gactatttac gatcaattga gtgcgaaaca aaagaagatg accaaagtat tgataaatgc
     1441 agaaggtaag ttatcccacc cactaagtat tcatgttcgt aatgaatggc aattggcaga
     1501 tagtcaatta ttatttcaag aaaaataaca ttttattttc tcatgttact tataagtagt
     1561 tttacatgct ttttgacttt aaaaatacat gtgatgatac atgttatatg aattatcaat
     1621 gtacccagta atatgctatt ttcattatgt aaaaaataac agggggtgtg tagatggaga
     1681 ttgatgaaac ttgtttgagt aactcttata aaagtatgaa tgactttttt gaggaaatgg
     1741 aactggattc agaattgctc tatcaatttt atcttgcttt tcgagggcaa cagatttctt
     1801 ttccaatgaa aatgtacgat cgtgagttag ttagaaaaag aatcgaaaac atgatagaac
     1861 aggaaaaaac tgtagatatc cgtcaattga cagaagttta tggtttctct gcaagatgga
     1921 ttcaaaaagt aattaaacaa aaagaaagga gacgagtaca tggctaagct gattgtatta
     1981 ggtggtttaa tttacggtgg atattggtta tatcaatata tgcttgcgca tccgaatata
     2041 gataaaggaa aagtaattaa gacagttggg gtaatcatag ttggactcat agctctaact
     2101 aagaaaaata atactaatag cccaaaaata atgagaactt atcgctgtag aggatgtgga
     2161 agggaaattt ctactagttt taatccatct gagagtcctc aaggaagaga ccgctgccat
     2221 acgggctatc gacattattg ggaaagaatc gattaaaaga ttaccattta gatcaattaa
     2281 ttgtctgata gaataagtac gtgagggaga aattgaaagg gggaaatata atgaaaaaat
     2341 ggctgttatt actaatagga atgggaattt tattcactgg ttgtacatct gagaatacga
     2401 ggactaaagc gactatagaa actgatacaa caattgagac taaaaaaagt aaaacgcaat
     2461 caactgaaca aactacaaca gaaatgaagc aatctgtgga aacaagtgca agttcaatca
     2521 ctgaatcttc ttctgaagag gttaagaata ctttgtggga tacgaataaa gcttccaaat
     2581 tagaaacttt tgtcacacaa tggggaaaga cattaggtca agaatataaa agctacaatc
     2641 ttcagaataa tgtaagtctg tatggcactc ctttaccaca agccgtgatt aatggcgatt
     2701 ggaaaatggc aatcaatgaa gcaccagtta ccgtacaatg gtcagaagat ggaactggtc
     2761 atgcagattt caatttagta gcagtttatt ctgatgtaga aactggagaa tatttagggc
     2821 agcatgttta ctttttcggt tttcaaaatg gacaacctaa agtttattta acgcaacaaa
     2881 atcaaggtaa tgaaaataat tatctgtact ttaacgaaac tcaaaatcaa cagttaaaac
     2941 aaggtttcag tgatattgtg aatggacaaa ctcctcaaac accagtagta gaagaatcta
     3001 ctcaacaagc aacaactaca acgacacaat ctacaagtag ttcaggaatt tcaagttggg
     3061 aagctgctaa agaatctgtg ctaaataata aagctttatg gttgtatgat tctcctgata
     3121 aaaatgtatc aaatactaat ttaattagca caaatgaata tatgaaagat ctaatgcaag
     3181 atgaaaatgg aagctactat tcaattaata ttgtaacgga tgatgatgga aacacgacca
     3241 tggctggtgg aaaacaattt cgtgtttata cagatggaag aatttctcaa cgtattggaa
     3301 tgactaacaa ttttcaaatg attaaataat tgaaaatatt atttatcaaa gaa
//
LOCUS       DANKMN010000139         3221 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000139 DANKMN010000000
VERSION     DANKMN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            301..585
                     /locus_tag="PW224_002850"
     CDS             301..585
                     /locus_tag="PW224_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909467.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="PW224_002851"
     CDS             588..1004
                     /locus_tag="PW224_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909468.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="PW224_002852"
     CDS             complement(1141..1860)
                     /locus_tag="PW224_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2909469.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="PW224_002853"
     CDS             2535..2630
                     /locus_tag="PW224_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2909470.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="PW224_002854"
     CDS             complement(2932..3177)
                     /locus_tag="PW224_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909471.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DANKMN010000140         3188 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000140 DANKMN010000000
VERSION     DANKMN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            186..1586
                     /locus_tag="PW224_002855"
     CDS             186..1586
                     /locus_tag="PW224_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2909472.1"
                     /translation="MLLFAVIPLLLSILLYSQQLLIYQQTIANIQKADAIALKVDQKV
                     LESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLKEISTLDVSLRTIDTLED
                     YQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVRVEIDLASKKNTTLVRSL
                     ILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSEQLSSGNLGFRLTLPETL
                     ELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQITPHFIYNSLDAIISLI
                     EQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHINDYLVILKIRYGHMLDYKI
                     DIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITTCKIDEMIQFKVIDNGIG
                     IPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGNDQKPITIDSKYREGTTVT
                     LMIPLSPKTIGGNKHV"
     gene            1579..3144
                     /locus_tag="PW224_002856"
     CDS             1579..3144
                     /locus_tag="PW224_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2909473.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
BASE COUNT         1072 a          623 c          558 g          935 t
ORIGIN      
        1 gagagactcc tttggtaggg tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa
      121 cacaaaatat tatagaacca aaaaatttaa gcataaaaca gatttttgcc caagcaaaca
      181 aagccatgct tctatttgct gttatccctt tgctactgag catactgtta tattcgcaac
      241 agcttttgat ttatcaacag acgatcgcaa atatccaaaa agcagatgca attgctttga
      301 aggttgatca aaaagttttg gaaagtatgt gggacttgat ttctggccaa gtaaccgtag
      361 atgagttcaa tgaggaaaat atcctagaag aacttaggaa tgatattcga gagatcaaaa
      421 ataatacaaa tacacttaaa gaaattagta cgttagatgt atcgctacgt acgatcgata
      481 cactggaaga ttatcaaaac gagctgatca ataatatctt ggcaaaagat tttatggata
      541 ccaatgaaga gatcatcaat caggtagatt ctgtcactca actgctgtca gatatcttac
      601 aagattttgt tcgtgtggag atcgacttag caagcaaaaa aaatacgaca cttgtccgct
      661 cgctcattct attaacaata gtggaacttg gagttcttag taccattttt ttctatgcga
      721 aaaagaatcg tcgcttttta gagagaaagg tccaagaacc gatcaatcag ctgatcgaga
      781 tgtcagaaca actttcttca gggaaccttg gctttcgttt aaccctgccg gaaacactgg
      841 aattacaaac acttaccgct agcatgaata aaatggcgga agatctaaac cagtctttac
      901 gggagaacgc tttgaaacaa taccatttag cacaaagtga agttcgtgta ttgcaagctc
      961 agattactcc ccattttatt tataacagtt tggatgccat tatttctttg atcgaacaag
     1021 actgctttga agaagctctg actatgaccc atgcactttc cggctttttt cgtacgtctt
     1081 taagcaaagg acaagactgg attcctcttg aaaaagaaat tagccatatc aatgattatc
     1141 tagtcatctt aaaaataaga tacggccata tgcttgacta taagatcgat ataccagaaa
     1201 cactttttcc tgtttctatt ttaaaaatga tcttgcagcc attgattgaa aatgctgtat
     1261 accatggaac gaagtacgtc cgtcacaaag gatttattcg tattaccaca tgcaaaattg
     1321 atgagatgat ccaattcaaa gttatagaca acgggattgg catccctgca gaaaaactgg
     1381 cagaaatacg taaagagtta gccaaaggga tcgattcaga tttcagtatc ggctacggac
     1441 tttacaacgt aaacaagcgt ctccttcttt attacggaaa tgatcaaaaa cctatcacga
     1501 ttgacagcaa ataccgagaa ggaacaactg tcacactgat gatccctcta tctcccaaaa
     1561 caattggagg aaacaaacat gtataaagtt tttatcatcg aagacgaata cctcattcgc
     1621 aataacttgc gggaacaaat aactgtcctt ggttcttatt atccaatcac ttatgtcggc
     1681 gaagctggag atggtgagat ggcactgtct tcaattatcg acttacagcc cgacatcatt
     1741 ctgactgata tccaaatgcc tttcatggac gggttgactt tttcaaaaga agcaaggaaa
     1801 ctacttcctt ggactcgtct catatttatt agtggattcg acgatttcga ttatatgaaa
     1861 ggagcaatcc aggtacaggc agatgattat ctgcttaaac cgattaaaag tagcgaattg
     1921 aaacaagcaa tagaaaaagc agtaactgct ttagatttgc agcaaaaaga aacgcacact
     1981 tcttctgatc ttgttttcga attgaaaaaa aatcatttct tgaacggttt gttcaaaggc
     2041 tcacttacga tcagtgaaac attgaaactt gctgaatctt tcaatcgctc tttagcaggg
     2101 aaaaaaatga ccgtattatt agcagcaaac caagtcagta cagactttga agattacgct
     2161 catctcagtg attatttgaa gtttttattt agggagaatc aacaagtgat cttctctgct
     2221 gtttcctctc gattcatcaa atttttaata gttgattctc ttgaagaaaa tgtgatcgag
     2281 accagttatc aggtagccca aacgttggtc catgaattag aaaagggaca aaaaaataat
     2341 ttttctgtcg ccattggtcc tgtcgcagaa agaatcagcg aattacctgc atcttttgag
     2401 atcacgcaga tgatacttga aaccgccagt tcccttaaag aacaaattat cagctatgag
     2461 gatttgattc aagaagcgaa acttccagtt gattctgtct tttactttga cctctcactt
     2521 gatttgtctt cattatcgaa aaacgaagta tctaagtaca tcgaacactt gacgaaacca
     2581 caaggcacac caactcgaac acgattatat cgtttatttg tcttgactaa gctactccag
     2641 catgtctgcc tgaaagaaaa gaaaatcccg caagaaatgg ttcattgcac atccaccagt
     2701 caacgcttag caatcagcgc cgacgactcg ctttaccgtt cgacagttta tatgttcatt
     2761 tctttcttaa ctgctctgcc ctcacagcct tcaaggaaca aatatcagcc aattttgaac
     2821 catgcattgg attttatcaa agaaaatttt accgatccag atatgtcctt aacttgggtc
     2881 gctcaacaag ttgcattaag tccctctcat ttcagtacga ttttttctca atcgtttaac
     2941 caaacattca tcgattatct gacagaacaa agaatcgaat tagcaaaaaa attattgcag
     3001 caatcagact atcgtatttc agatattgct tttgaaattg gttataatga ccctaactat
     3061 ttcagttatt tatttaagaa aaaacaaggg atttctccaa aagaatatcg gacacagctt
     3121 ttccttaaaa acaaaaataa ctaataaacg aaatccgtac aatgatactc tttccatttg
     3181 caataaaa
//
LOCUS       DANKMN010000141         3165 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000141 DANKMN010000000
VERSION     DANKMN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            149..457
                     /locus_tag="PW224_002857"
     CDS             149..457
                     /locus_tag="PW224_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909474.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            459..881
                     /locus_tag="PW224_002858"
     CDS             459..881
                     /locus_tag="PW224_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909475.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            901..2490
                     /locus_tag="PW224_002859"
     CDS             901..2490
                     /locus_tag="PW224_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909476.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            2502..2837
                     /locus_tag="PW224_002860"
     CDS             2502..2837
                     /locus_tag="PW224_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909477.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2978..>3165
                     /locus_tag="PW224_002861"
     CDS             2978..>3165
                     /locus_tag="PW224_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909478.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1264 a          454 c          640 g          807 t
ORIGIN      
        1 ctattacgtt tctcaaaaat aattgtggga acaaaatatt taaaaatagg atagtgaaag
       61 gatagcaaag gatagtaaaa ggatagaagc aggatagcga aaaggatagg ctccttttcg
      121 ctatcctttt taaattggag gaattaatat ggaagtaaga ataagaaatg tagatgagaa
      181 aatcgtaaga gaaattgatc gaagatgtga cgaactaagt aaccgaacgg gtaataaatg
      241 gagtcgtaac gattatttaa agctactcgt tgaaaatgat tttgatcgtc ctttgatgga
      301 atataaacaa gataaatttg atcaattatt aaatcagtta atagataaat tttcttttgt
      361 tcaaagtcat aatacaaaag tattagaaga atatatttgt cagaataatc aaattatcga
      421 acttttaatt gaacaaagta gaggaggaga agtttaacat ggccaaagtt atagacaagc
      481 atatttattt ttatgaaaca catatagatg ttctagatga aattattgca actcatgcag
      541 aggttaaaaa ctatagtgaa gccgttcggt ttctatgtat gaattatgat tcagaagtgg
      601 agaaaaaagt aaattccaat caagcaaagt tgaatgcaat gggaaaagaa atttctattt
      661 taaccgaaat ggtttcagag tttgcggatc tccatttgaa agatacaggg attttaattg
      721 gtacaaattc aactgtttac caagaagcaa aagagcgtgt ggaaggcaag ataaaagcaa
      781 atcaaacgaa gaaatattct cctaagataa agaaaacaat aagcgaatca gtgactacaa
      841 aaagtgaagt atttgatcca acaaggttct taaaacgata aaaattagaa ggagaatgtg
      901 atgagtaaat cacctgcagt cattctcact tgtcaattta ccttgcccaa taaaaaatct
      961 ttttcaacat acattgatta tatgacaaga gaaaaagcgc tggaagaaaa ggaacgacgt
     1021 acgcccgagg aagaacagga actgcgtcta atcaagaatg ctttagagga tttttatatt
     1081 cctgaaggag aaactttttc ggagagtaag acgggtaaag aattcagcga aacgtcgaat
     1141 gaagcgcaat cgttgatgaa acatggaatc aattttgaca agttagaaga acaagatttt
     1201 accaaatatc tttcttatat gacacgtcac tatgcgttag aacaaaagaa agtactgacg
     1261 catagtgaac aaaaagaata ccaacgcttg aaccaagcaa taaaaaaata tcaaacagag
     1321 ccacacaaaa aagcttcagg aaatcttcct ggagtttttt ctatggcttc agacgaagtg
     1381 aaaggaagtg atctgaagga gatcaagcaa accttccaaa gaggacaaca aaaaggctca
     1441 atcatttatc aagatgttgt ctctcatgat cattcgtatc ttgaaaaaat aggattgtac
     1501 gatcccaaaa cagatacttt agatgaagaa ggtttaaaag cagctggaag aaaaatgatg
     1561 gagacattgt tcgagaaaga gcaactgaat gaaacaggct actggatggc aacgattcac
     1621 cgaaatacca aacatatcca tattcatttt gcagttgttg aaaaaaaaaa tacgcgtaaa
     1681 ccatacactg agattgaaaa tggtgtgagt tatatcgtac cgaaagggaa gagaaggcaa
     1741 gcaacattag acgaaatgaa gactaattat gtacatgagt tagaacgttt ttcttatgaa
     1801 aaaaatcgaa tgggtatgga gaaacgaaat ttattgacac ggaaaagtga tttgcgtaac
     1861 accttaacca aagtagtgaa agagccagta cgttatgatc gacacgcaat gagcttatta
     1921 actgaagtat ataaaaactt acctaaaaag cgttcggaat ggcattacgg ggatgaaaat
     1981 cgaaccaaac tttcaccata cacaagagag aaactcgatg agttaaccca atatgtgtta
     2041 acaaaagaac ctggatatca agaatatata cagatttcaa aagcattgaa agaagaagca
     2101 aaaagtcttt atggggaaag caagagagaa tcaaaggatg cagaaaaaaa tgctctcttt
     2161 gatttaaaaa agcgcacagg aaatgccatt ctaacagaac tgaagaaaca ggatagtcaa
     2221 ataaaacctt taatagaaag gatggatcag atgcaatcta tttacaatga acgatccttt
     2281 cgaacactta aacagttgcc aacgaatgaa tacaaatata gttacgaaga attcatgaag
     2341 cgcaggaaac aattccaaca aagagttgtc acgcaacgga gtttgcgtca attaaatcga
     2401 acgattaatg cccatgaaga aaaataccac gcaagtaaag catacgaaga gcaacaacga
     2461 agaattcaaa tggaacaaga aagacagtag gaggaatagc aatgaaacaa tatttagatc
     2521 aatggaaagt tatagaagga tcattaagag aagaacgaat cgaacaactt cccgattgtt
     2581 tagaaaaaga gcatttgttt cagataagag aaatgttgat aaacgaacag tttgatccaa
     2641 atcaattttt agtcgttgag tacccagcta ctggtgtgta ctgttgtaat catgtgaagg
     2701 gtgaaaaata ttttatcatt caagaatatg agggcaagtt agcgccttat tatactactt
     2761 gggaaatgaa tgaagagggg atcaataact tcccgtgtaa aagcattgaa gagtctattt
     2821 cactcacaga atgttagttg ttaagtgaat aaaagaagta aaagagtcct gaacgtgatt
     2881 gtccaggact cttttttttc actgaattgt tttccgccaa ttcattgagt ggtgcgattt
     2941 ccgtctacat tatatcaaaa gaaaggagga agaagaaatg gcttattcaa cgattggagt
     3001 tgttgttgaa aaaagcagag acaatcttgt ttttgtgaca gaaattcaaa caggcagagc
     3061 ttttgtagtc acagacaaag cggcaaaagc atatcaaagt ggcgatattt taacgttaaa
     3121 tctgactacg aagacgttcg tcgatgcggc agaagattat ccatt
//
LOCUS       DANKMN010000142         3012 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000142 DANKMN010000000
VERSION     DANKMN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            49..1161
                     /locus_tag="PW224_002862"
     CDS             49..1161
                     /locus_tag="PW224_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2909479.1"
                     /translation="MLFSLSAFSTGTILGWQFYNNEHSQIVAHAQSAQKKLILTTNEQ
                     QLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKA
                     LTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTD
                     EEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHT
                     KFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQV
                     LSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAI
                     IMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1428..2342)
                     /locus_tag="PW224_002863"
     CDS             complement(1428..2342)
                     /locus_tag="PW224_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909480.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(2400..3012)
                     /locus_tag="PW224_002864"
                     /pseudo
     CDS             complement(2400..3012)
                     /locus_tag="PW224_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1121 a          476 c          446 g          969 t
ORIGIN      
        1 ggaaaacatg ctaaaaaaag aaaatattca tttttaatga tcgttgtcgt gttgtttagc
       61 cttagtgcgt tctctacagg aactattctt ggatggcaat tttacaataa tgaacactct
      121 cagatagttg cccatgctca atcagctcaa aaaaagttga tactgactac caatgaacaa
      181 cagctcgttg aaaaaatcga tcaactatta agaaaaaatg attatattgg ctcaatttac
      241 gtaaagaaaa ataatcgcat catcatggaa aaaggctatg gctatgcgaa taaactcacg
      301 caacaaccaa atgatccttc tctttattat cagattggtt cggttcaaaa agcattgact
      361 actcttttga ttttaaaaca agtcgagcgt gggcaccttt ctttagatac taagctggct
      421 caattctatc cacaaattgc tggcagccag caaattacta tccgtgactt gctgtatatg
      481 cgctcaggct tgcaccgaac tgcttctcca accataccga tgactgatga agaagtcgtt
      541 caatttgcga ttcagcactt aaaatttaca gattatcaga cctctcatta tgattcattg
      601 aactttacca tattgactgg aattttgatc aaattaacaa atcaaccgta tgaaaaatta
      661 ttgaccgatg atatcatcga acctttagga ttacagcata caaaatttta tgaccaagtc
      721 aaaaattcat ccaatcatgc actatcctat caaatgtcta aagaggatga ttactttaaa
      781 ccactgacag aatctgaaac agagatccat aacgaattag ggactggtaa tatcagtatg
      841 tccgtctatg atttaaatac gctgtttaca caagttttat ctggaaaact agtttccaaa
      901 gacttactct tctcattatg gcgtgaaaat gcgaatggac atccatatag tggtggggta
      961 tatagcggag ataattatat cctgtcgcaa ggaaatatcg actcttttga tacatctgct
     1021 atatttagca aagattcaca ggatgctatc atcatggaaa gcaatataaa agctgacaaa
     1081 actatcaaac tgcctactgc tgatctaaga aaccaaatct atgacctgat ggaaggaact
     1141 actaagttgg caaatagctg atatgatgaa aagatagcaa atactacttc tttgaatagc
     1201 atttgcttaa aaaaagtact cgactaaacg taggaaagag tgtgggacaa atctaaaaat
     1261 caatttttgc tacagacttt aattccaaat aaacagaaca gaaagaggtg ttcttctttc
     1321 tgttctgtcc aaattattct aagatttctc tttaccaaca ttatttgaca aagggcctat
     1381 taaatctaaa atagtatctt tagattgcta aaatatgtgc tagcaaatca ttcatggctt
     1441 gttcttattt ttaattttat gcaataaaaa agaagaacaa ttagcgtgat tatagaaata
     1501 aataatgaga ttttagaaaa aaatgtgtat gaatacctga ttgttacttt atttattcca
     1561 ctttcacttt ttactgtaac aaaaggacct ccttgtttta gtactgtagc attttttgaa
     1621 ttaacagtaa ccgttaatcc tttataccgg aatacaggta aaactattgt tttgcctttt
     1681 gaagatttaa acgtaaatgt aatttcatct ttatttggag aaaagctact ataaataact
     1741 tgaccatctt ctgtaacttg atggtttttc aaaagcaatt gatctttttt atagcctata
     1801 ggtacataat caaaattctc aaattcttca agtttatttt ccatttgttc attagaataa
     1861 gttccttgaa aattcatttc tttcaccatg ttaactactg atgaataatt taaaattgaa
     1921 atagctaata ttaataatag tataaaattt cttctccttg tataagaaaa tttttccaac
     1981 tgtggagaaa taaatagaga tactgaaaaa gatatgaaca atgttgaaaa tatcataaaa
     2041 cgccaaggag cttgaataat atttaaagga gtattttgta aggcaaacca tggaaatatt
     2101 tttgtacaaa gtagaataga tattataccc attatataag tgattttaga taagattcct
     2161 aattttttaa agttaaatgt tagtataatt aaacaaataa gcaaagttaa gccaatacca
     2221 aatgatagta attctgaatc tatacttcca atcaaaaact ctttacccag ataaattgct
     2281 cttttttgta atatatccac ataaggtcta ttcagatctt gatacaatgt ctgctcaatc
     2341 ataggaccta taaacgctag tgaaagaagt aaagtaacta tagcagcttt tattaattta
     2401 ataattaaga agatttctat tttctttttc cgataaaatt gaataattaa agcaattata
     2461 atcattacca tagtcatagc tacagaaaga acatgagtgt aaattaataa agtcatgcta
     2521 atcgccaaca taacccaaga ttcttttttt gagtcgtaaa gctttataaa cccgagtaaa
     2581 ataagaggaa gtattgacat tgcgagtaat tcccccattg cccctcgaag aaatatttct
     2641 acactcctaa aagaagatgt tgtatatata atagcgaaaa aggagctagt aacatgtttt
     2701 ttggtaattt catacattac ataatgacat ataaatagag taatgagcgt aacaatataa
     2761 agaaaaaggt tatagcctat tgctaaatta gtaaacttga tcaataaaaa taaaggatat
     2821 agagttaacc atgggtaaaa attatttact tgcatgccat atgatctaaa cgtttcaaaa
     2881 ttaatagggg agctaaaaat agattctaaa cttaacgcac gattaatgtg aaatgccgta
     2941 tcataaaagc caaatatttc catgttttta aatgctggaa ataaatataa taaactaaca
     3001 aatgcataaa ct
//
LOCUS       DANKMN010000143         2952 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000143 DANKMN010000000
VERSION     DANKMN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            89..406
                     /locus_tag="PW224_002865"
     CDS             89..406
                     /locus_tag="PW224_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2909481.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="PW224_002866"
     CDS             complement(497..862)
                     /locus_tag="PW224_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909482.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="PW224_002867"
     CDS             1070..1552
                     /locus_tag="PW224_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909483.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /locus_tag="PW224_002868"
     CDS             1749..2639
                     /locus_tag="PW224_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDL2909484.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1008 a          460 c          566 g          918 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttagac cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2941 cagaccttgt tg
//
LOCUS       DANKMN010000144         2796 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000144 DANKMN010000000
VERSION     DANKMN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="PW224_002869"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="PW224_002869"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2909485.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1085 a          435 c          569 g          707 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattgg
//
LOCUS       DANKMN010000145         2791 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000145 DANKMN010000000
VERSION     DANKMN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(60..530)
                     /locus_tag="PW224_002870"
     CDS             complement(60..530)
                     /locus_tag="PW224_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909486.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            617..1315
                     /locus_tag="PW224_002871"
     CDS             617..1315
                     /locus_tag="PW224_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL2909487.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1493..1834
                     /locus_tag="PW224_002872"
     CDS             1493..1834
                     /locus_tag="PW224_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909488.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1827..2498
                     /locus_tag="PW224_002873"
     CDS             1827..2498
                     /locus_tag="PW224_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2909489.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2504..>2791
                     /locus_tag="PW224_002874"
     CDS             2504..>2791
                     /locus_tag="PW224_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909490.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTG"
BASE COUNT         1013 a          417 c          562 g          799 t
ORIGIN      
        1 ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact
       61 cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt
      121 ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta
      181 ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga
      241 atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca
      301 attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct
      361 aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc
      421 atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt
      481 atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa
      541 attgttaagt gaaaaaaatt caaaataaac accaccagtt tttaactaaa ttataccaaa
      601 aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac
      661 gagcttacaa gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa gagatattga
      721 tgcactaaaa aaagatgtgt ccaattttgg acagatgttt gtagaaggta atgaaccaga
      781 ttcatatggc agaaatcgac gagttttctt cattagtaga gatggatttt tcttgctggc
      841 tatggggttt acaggaaaga aagctatcta cttcaaacaa aaatacattg aagcattcaa
      901 cgaaatggaa gatgttattc gcaagaatac tgttcctcaa acaattgaag atatgatgat
      961 ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat
     1021 gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatggg
     1081 agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa
     1141 attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa
     1201 gagattcgat gatgctgttt catttattga aatgtggttg ccagaaactg cgatccgtat
     1261 ggaaattgat caactgaaca gacaacagag actttttggt gatgacaatg aatagagctg
     1321 aagcgctaag aatagggacg gcaattgcta attgctggtg gaaatactat aaaccaatca
     1381 tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc
     1441 gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc atatggaaaa
     1501 agaactttcc actctagatc aatatttgat tgatcctgat tggggcaagc cgaaaattga
     1561 ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac tagcttggga
     1621 tcaagatgat ttaggcaacc atgtaactat ttgggatcat gtttatctta tccatctatc
     1681 gaagcattcg aataaacctg aatatattta cgtcatcgaa gatggcttga ttgatgcgct
     1741 agaagagtac gacagagata acttgattga tatctcttat tacggaccag gtaagaaata
     1801 cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa
     1861 aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat
     1921 cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta
     1981 atgagtacaa acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat
     2041 ataaaccttt tcttatggat gaaactggga cttatgtatc tgtctcagta acggttaaag
     2101 gacacacgga aaccgaatta tttcctgttt tagattatcg aaaccaacca gttgttaaac
     2161 caaatgcaat gcaaatcaat aactcattga agcgatgctt tgtgaaagca ttggctctac
     2221 acggactggg attatatgta tttcaagggg aagatattcc aacaccacct agaatcgata
     2281 caaagaaatt aaacatgcta gagacgattc tagaagcttt caatgagcag atgggtaaag
     2341 atatgaccaa aaccttaatc gaatatgtta atgagcagac agataaatta gggctcttag
     2401 ctgataacgt tgaaactatt gaacagttaa gctatgagca atgtgccttg atggagcgag
     2461 caatagcagc taagagaaaa gaattagata agaagtgata tgagtgttta aaccattaat
     2521 cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg caaccatcaa
     2581 tgaggaagtg aacattgatc gactaaagac gatgtatgac ggctacgatg gtgatcgagt
     2641 cattgaaatt cgttttattg atcctagacg tttcaccgta cagcaacgaa acttcatcta
     2701 tgcgctgata ggcgatattt ttatcgatac aggcatgcca acggacttct ggaaggaatt
     2761 cttctacttt cgttttgaag gtgtcacagg g
//
LOCUS       DANKMN010000146         2707 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000146 DANKMN010000000
VERSION     DANKMN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(1150..1668)
                     /locus_tag="PW224_002875"
     CDS             complement(1150..1668)
                     /locus_tag="PW224_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL2909491.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          899 a          486 c          377 g          945 t
ORIGIN      
        1 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
       61 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
      121 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
      181 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
      241 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
      301 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
      361 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
      421 tatgaaatga aagacctttt acatcttgca gaaatcgtta ctgggattaa actaagcaat
      481 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
      541 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
      601 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
      661 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
      721 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
      781 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
      841 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
      901 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
      961 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     1021 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     1081 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     1141 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     1201 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     1261 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     1321 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     1381 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     1441 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     1501 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     1561 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     1621 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     1681 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     1741 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     1801 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     1861 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     1921 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     1981 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     2041 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     2101 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     2161 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     2221 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     2281 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     2341 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     2401 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     2461 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     2521 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     2581 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     2641 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     2701 ccactta
//
LOCUS       DANKMN010000147         2451 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000147 DANKMN010000000
VERSION     DANKMN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            288..542
                     /locus_tag="PW224_002876"
                     /pseudo
     CDS             288..542
                     /locus_tag="PW224_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            612..1124
                     /locus_tag="PW224_002877"
     CDS             612..1124
                     /locus_tag="PW224_002877"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2909492.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1121..2158
                     /locus_tag="PW224_002878"
     CDS             1121..2158
                     /locus_tag="PW224_002878"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2909493.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            2318..2451
                     /locus_tag="PW224_002879"
                     /pseudo
     CDS             2318..2451
                     /locus_tag="PW224_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          888 a          451 c          492 g          620 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacaccgac catcgtccag taaagagacg
     2341 caataaattc tatcgaagtt tacgcactgc ctctacaacg attaaaggca tggaagccat
     2401 ccgaggatta tataagaaaa cccgaaaaga aggcactctc ttcgggtttt c
//
LOCUS       DANKMN010000148         2402 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000148 DANKMN010000000
VERSION     DANKMN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            207..2069
                     /locus_tag="PW224_002880"
     CDS             207..2069
                     /locus_tag="PW224_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2909494.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..2401
                     /locus_tag="PW224_002881"
     CDS             2069..2401
                     /locus_tag="PW224_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2909495.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          716 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
     2401 at
//
LOCUS       DANKMN010000149         2365 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000149 DANKMN010000000
VERSION     DANKMN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..1368)
                     /locus_tag="PW224_002882"
     CDS             complement(join(<1..795,795..1368))
                     /locus_tag="PW224_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HDL2909496.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIK"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="PW224_002883"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="PW224_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL2909497.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          703 a          449 c          451 g          762 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 atcttcctag aaaatcactg gatta
//
LOCUS       DANKMN010000150         2330 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000150 DANKMN010000000
VERSION     DANKMN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            287..694
                     /locus_tag="PW224_002884"
     CDS             287..694
                     /locus_tag="PW224_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909498.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="PW224_002885"
     CDS             708..1109
                     /locus_tag="PW224_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909499.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="PW224_002886"
     CDS             1111..1482
                     /locus_tag="PW224_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909500.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1823)
                     /locus_tag="PW224_002887"
     CDS             complement(1518..1823)
                     /locus_tag="PW224_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909501.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDLYKVWDTEVVFLAIETSLLVN
                     LHYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          875 a          316 c          394 g          745 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacat cagtcacgat atcaaacaaa
     1621 aaatatacgt ctttcttcat tctagttttc gcagcaggga ttttaaagta gttcttatta
     1681 gaataatgga gattgactag caggctagtt tcaattgcta aaaagactac ttcagtatcc
     1741 cagactttat ataaatcttt gacaaatttt tctgatggat cgtatttaaa ccaaagcaga
     1801 tgttcctcta gctcgattgt catgtttttc acctcaacga aagtatacga actaatgttt
     1861 gttttgtaaa ggataaaaat agggtagcaa ccggtaccca aactagtacc catttatcaa
     1921 aaacgacgaa atttatgtaa atagaaaaat aagaataaac attgatgtat caacatttat
     1981 atatctttag aacaaatgaa aagggtggtt ctcataagaa aaacatgttc gcacttctag
     2041 gcaaaccagg atttgaagac cttgctaaag aattgaacga acgcctgtaa aataaggatt
     2101 tgtcgctaga tttaaaaaat cagtgataga aacatgtttg ctttaaaatg aaactagtga
     2161 tagttttagt gatagtgaac ttaaaaaaag cactcaattt gagtgctttt ttattgttat
     2221 taaaaattaa aaaactaaaa tacatacccg aatttcatac ccgaaatata aaaaatgtca
     2281 tcacgtacat ctttttcgaa actaccgaag tagccaaaga tgtactgatg
//
LOCUS       DANKMN010000151         2293 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000151 DANKMN010000000
VERSION     DANKMN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..750
                     /locus_tag="PW224_002888"
     CDS             <1..750
                     /locus_tag="PW224_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2909502.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
BASE COUNT          833 a          302 c          463 g          695 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaacg
      721 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
      781 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      841 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      901 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      961 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
     1021 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
     1081 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
     1141 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
     1201 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
     1261 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
     1321 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
     1381 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
     1441 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
     1501 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
     1561 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
     1621 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
     1681 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1741 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1801 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1861 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1921 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1981 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     2041 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     2101 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     2161 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     2221 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     2281 caaaggagtg taa
//
LOCUS       DANKMN010000152         2254 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000152 DANKMN010000000
VERSION     DANKMN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..1301)
                     /locus_tag="PW224_002889"
     CDS             complement(<1..1301)
                     /locus_tag="PW224_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2909503.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPY"
     gene            complement(1435..>2254)
                     /locus_tag="PW224_002890"
     CDS             complement(1435..>2254)
                     /locus_tag="PW224_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2909504.1"
                     /translation="GTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYN
                     PEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIVVVTYPDGS
                     KEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQYHGTADKN
                     GNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDNKTDNQGSK
                     QNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          661 a          422 c          290 g          881 t
ORIGIN      
        1 ttatagggtt ggctaaaact aggatgaaat aaataatcta acattttcaa tttgtctaat
       61 gtaatttgtt cttctatagc aagagaaaac attgataata gtccagataa atcttcttga
      121 ctacaaagtt gtacacctac tattctcttt gtattttttt caaagctaat tcttaatttt
      181 acacgactat tttttctcaa aaaaacaggt ctaatgaaat cttctatttc tatataatct
      241 atagactcac ttaataatcg tgctgttttt aaattcaaac ctgctgcgta aaaattgtaa
      301 ttaaatatag aaaagccatt tgtaaattgg gaaccagaag gtgtaaaatc ttgattgtca
      361 atatgttgag ttgcaatata agctgttctt agagcattag aaatagaaaa atcaacacta
      421 cttctattaa tggacgtaaa aaaagttgta gcacaatccc caatagcata tatatctttt
      481 atatttgttt gctggtattc attagttaga tatgctccac taacatctct caagagttcc
      541 tttttgccaa ttggactatt agatagaaat cctatagctt gtacgaccat atccatagag
      601 tattgggtac cacttgtagt gactattttt tcaatctttt ttcttcctaa atatttttta
      661 ggactttctg atagacaaag atgtattcca ttttttgata gtatttcttc aactttatct
      721 gaaaaactac ggtcgtaata agtcgacaac actctatctg caacatcgaa aagatatact
      781 tctttccctc tttttcttat ggcttcagca atttcaactc caatatatcc tgctccaata
      841 attcctactt ttttaatagt attatcatct aaagctttaa aaatctcttt agcatttgga
      901 aaattcttta tcgaaaaaat attagctaaa ttattccccg gtacatctaa cataatttgc
      961 tttgaacctg tagctagtat aagtttgtca tattcttggg aaaatcttgt gccatctttt
     1021 tttacacaaa taatcttttt attttgaata tcaattttgt caactgctgt ctccaaaaac
     1081 aactttttta tcttagaatt tagttctctt ctattttgat aaaaaagatc ttctaaattg
     1141 ctaacttctc tttttatgaa caacgatgtc ccacattcta agtaactaat ttctgatttt
     1201 ttatctatca gaataacttc ataatttgaa tcacataagt ttagaaatcg agctgcagct
     1261 agccccgaat ggtttgatcc aataattact atttttttca cttaatcaca ttcttttccc
     1321 ttctatgaaa agtatcttca tttttaacta acaacctatc tcccaaaata aaaaacaaaa
     1381 agaggaagaa aaagattctt cctcttttca gagaagattt aagaaattga ttttctattc
     1441 attctctttt tttcgatttt tataccctaa taaccctaaa gttcctaaca ggattaaacc
     1501 accactaata gtaaagatat tactatcaat ctctcccgtc ttaggataag cttttgagga
     1561 ctgcttttga ggactgcttt tgcctctatt tttgttttgc ttgctacctt gattgtcagt
     1621 cttattatct ttactaacgg ctttttcact gtctttttcg tttgaagaaa caactacatt
     1681 agtaggttca ctagtattac cagattcatc agttgcagtt actatcactt ttgttcctgc
     1741 ctctaattta ggaactttaa ctgtaaaatt accattttta tcagctgtcc catggtattg
     1801 actcccgttt ggtaatgtaa ctgtcacctc tgcatttggt tcagttttac cagtaacaat
     1861 ttggtcaccc tctgtaatag gatcaacttg aggtttattc ggtgcttttt tatcttctac
     1921 agaaatagta acctctactt cttctttaga tccatctgga tatgttacta ctacaatccc
     1981 ttttttgttt ccagctgtac taacatctac tttctctttc caagtataag tagttccttt
     2041 tggtagatct tctttgttct taattccctc agatgcgtct ggctctttat ttaagtctgt
     2101 agtcactttt tgtccttcag gattgtattt atcggcatcg gaacgattgt ctgtcacttc
     2161 tactggaact ttcactgtat ctttcgtacc atctggatac gttacttcga taaccccttc
     2221 gtaattacct ggtgtgttgg tatcaatcgt acca
//
LOCUS       DANKMN010000153         2227 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000153 DANKMN010000000
VERSION     DANKMN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..159
                     /locus_tag="PW224_002891"
     CDS             <1..159
                     /locus_tag="PW224_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2909505.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="PW224_002892"
     CDS             242..859
                     /locus_tag="PW224_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909506.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="PW224_002893"
     CDS             1023..1355
                     /locus_tag="PW224_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909507.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="PW224_002894"
     CDS             1679..1840
                     /locus_tag="PW224_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDL2909508.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2227
                     /locus_tag="PW224_002895"
                     /pseudo
     CDS             1872..2227
                     /locus_tag="PW224_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          788 a          292 c          451 g          696 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggacattgg cattatcttt atcgtgcaat tgatgcggac
     2161 ggcttaacct tagatatctg gttacgaaag aaacgggata cgcaagcagc ctatgctttc
     2221 ttaaaac
//
LOCUS       DANKMN010000154         2056 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000154 DANKMN010000000
VERSION     DANKMN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            493..714
                     /locus_tag="PW224_002896"
     CDS             493..714
                     /locus_tag="PW224_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909509.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="PW224_002897"
     CDS             715..972
                     /locus_tag="PW224_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909510.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="PW224_002898"
     CDS             complement(1108..1710)
                     /locus_tag="PW224_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2909511.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="PW224_002899"
     CDS             complement(1777..1932)
                     /locus_tag="PW224_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2909512.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DANKMN010000155         2016 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000155 DANKMN010000000
VERSION     DANKMN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            734..1066
                     /locus_tag="PW224_002900"
     CDS             734..1066
                     /locus_tag="PW224_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL2909513.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1685..1915
                     /locus_tag="PW224_002901"
                     /pseudo
     CDS             1685..1915
                     /locus_tag="PW224_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
BASE COUNT          688 a          291 c          416 g          621 t
ORIGIN      
        1 tatgagttaa aatattatga taaaaatcac aaacgtagct ttttacgtca taagtgttaa
       61 tagttaataa ttaatttcca acacaaaaaa gagaacaaac gtttgcatgt tgctgttttt
      121 tagttataat aatacgcgaa gagaggtaat ttcatttttt aaaaaagcta aaaaccttga
      181 tattacttct tttcaggcaa tacgaatatt attcgcttac atttttttgt ataaatttag
      241 ttgttttaaa tttaattgtc tgctacgctt gtgatgaatt aattttcgtt ctgcactttt
      301 ggacgataaa tttggacaat tgaaaataca aaaaaaagaa aggccctgtg agactcggaa
      361 ggctggtaac cttcgtcgac gtccacaggt ggcattaaga caacctttaa tcccttaatg
      421 cgtttacctt tcagtgttac ggtattaccc gcaacggatt tagtataaca gatattcaaa
      481 agtgatacca ctttttttgg ctgtgttaag gttaaaaata cgtgatatta cgatgaatct
      541 atgcctaagc ataatgttat aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg
      601 aaattagagg gaacgcatgt ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa
      661 tcaattctct atttttgaaa aataacaaga aaaacttatt aaaaatattt aaagtaagtg
      721 ggtggattat tttatgtcat atacaggaat tttgagtctc gaagatattt gtcactacgg
      781 taaacggtgt accgctacag agaaaatcac gaagaaacta tcaactggcc aaagtaaaac
      841 cgttgtgcag tgtaagaaat atataatcca gaaagacaaa gtctcagagg aaatgattta
      901 ttacgctggt aaacaaaagc aaataatttt aaaagatcct atccctttaa aagaactata
      961 tccaacaatc aaacatgttt atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg
     1021 agtcttgcgt tgtaccgcaa aagggatggg gcgtttaatc agctgaattg atcattctca
     1081 taacttgtat tttatcgttt ttcgcttgct tgaaaatatt gtcgagactt ctaaaagaac
     1141 ttagaattac tagaaaaata aggagtagac ttgatctatc aacaattcct ttcggggggt
     1201 gacggggtag gtttttacga ttggtaacgt aaaaaattgg tcaatcgagt aaatgccctt
     1261 gtttggatag tggtgaaatg aatttgtgta aataatatgc acagagtaaa caagagggaa
     1321 tttagaagtt agacaagtta gaatagcgcc tagattaatc atgagcagct actaagggat
     1381 ctaggctttg acgcaaactc atggaataag gagtaggtct tctcgtaatg gggaggacgc
     1441 acaagaaatt gtgtcgtgtg ctgcagtaca tgtagatact tgcgaggacg ttggtcattg
     1501 gatcaatcga gcatttaccg atttctacta gggtttctcg tttcggacgc cataaacgag
     1561 tcgatatttt ttatgtattt ttaagtaaac gaaaaacgat aaaatatgaa cgagtcctga
     1621 ataaagtgaa aaaattgata taaaacatag agaaaatgaa atttacgtaa tggaggaatg
     1681 acaaatgaaa aattatgatc cgaatatacg attgggcaca catactatta aggtttcttt
     1741 ccaacgttgg gattacaaag gatttgtgac gtttagaaga ggcggaaatt gtaaaggatt
     1801 agatgtttta gcattagacg aggatgattt atactatcaa aaattgactg ataatccaat
     1861 tggattcggc ttgctacctg aagatgatga aggtaatgaa tggtttaaca gaattaggca
     1921 gaagtcgccc atcctctata ggtgggtgat gaatgccgtt cggtatgagg gttaccgttg
     1981 gtaactcgaa gaccgacaac taaggcgtat tgcctt
//
LOCUS       DANKMN010000156         2004 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000156 DANKMN010000000
VERSION     DANKMN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..378
                     /locus_tag="PW224_002902"
     CDS             <1..378
                     /locus_tag="PW224_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2909514.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="PW224_002903"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="PW224_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2909515.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2004
                     /locus_tag="PW224_002904"
     CDS             1859..>2004
                     /locus_tag="PW224_002904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909516.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFT"
BASE COUNT          555 a          340 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttac
//
LOCUS       DANKMN010000157         1983 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000157 DANKMN010000000
VERSION     DANKMN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            714..1799
                     /gene="xerS"
                     /locus_tag="PW224_002905"
     CDS             714..1799
                     /gene="xerS"
                     /locus_tag="PW224_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL2909517.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(1870..>1983)
                     /locus_tag="PW224_002906"
     CDS             complement(1870..>1983)
                     /locus_tag="PW224_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2909518.1"
                     /translation="IKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          722 a          338 c          279 g          644 t
ORIGIN      
        1 aaaccactag cccgccagct agtggttttt ggtgtatcat gaattacctt ttcgttaaaa
       61 caattatacc atatttccta aaaaatacaa ttaatagacg aattatcatt accactttga
      121 tgaaaaaagc tgacataaat ttttctttta tttttataat gaataaacat ttatcaataa
      181 gcttttgaaa ggcaggatcg tactttgttg atgattgaga tattgctaca tcaatctcag
      241 cagttaagat ttttttgaat tgttgcttga attttttctt tatctaaaaa aattgtatct
      301 ccaggatgca acccattttc tttaagaaat tccttttaaa ttgttaatac tactgagtgc
      361 cccacttctc tgaattttct ttaacttctc cctccccttt tttatttgta tatacattca
      421 tatacgcttg ttaatatcaa atcaaccgta gtctctctta tgtctaatta tatagaaaga
      481 ttgttctttc tatataattt agacaattta aaaaattaag attgcctcct tctgtttcct
      541 ttcctattca tgtataccat ttattttaaa ttattaaatc tatacctatt tatctgcttt
      601 tttgaaataa attaataaat ttcagagtat accaattgac aaaacactct ataattggta
      661 tagtccaatt atagagatac tcctctcccc tacttatcga aaggatgaat aacatgaaaa
      721 atacagaaat atatcaaaaa atagatacaa tcatacttca tatgcccgat tatataaaag
      781 attttgttca agatcgtgag gataaagatc aatcacctcg aactacttta gaatatctaa
      841 aaaattataa gctcttttat gaatggcttc tgagtgagtc tatcgttccc gatacttcaa
      901 tcacctcgat aagacagtta actgcctatg atctaaactt gtacaaatct catttgaaac
      961 gtcgcacaaa ggaaaatgta aaaaaagaga caacaaaaac taagctcaaa gataataata
     1021 acttaggtct atctacttct actatcaata gaaatatcac agcattaaag gttctattta
     1081 aatatctttc gaaaagttcc aataatcctt taggaaaacc atacttagaa aataatccta
     1141 tggatcaagt agcaactatc acagataaaa ctacgttagc tgcccgtgca aacgcaatcg
     1201 agaaaaaatt atttctagac gaagatacac aaaattatct agattatatt gccaatgaat
     1261 ataaaaaaac tttgagcaaa cgagctttga tctattatca acgagatgta gaaagagacc
     1321 tggcgatcaa tgcattgatt ttaggatctg gattaagact ctccgaggtt gtaaatatta
     1381 atttagagga cctatcttta gaaaagaata atgtggtagt aactagaaaa ggaaataaga
     1441 gagacgcagt aaatatagca gcatttgcaa tggaatattt agctaattat ctagctattc
     1501 ggaaagaacg atacaaagta acagaaaacg aaaaagcttt atttctagca atttatcaag
     1561 gagaagccaa gcgtatatcc ggtattgcaa tcgaacggat ggtagcaaaa tattcaaaag
     1621 gattcagagt ccaagtcagt ccacataaat tacgtcatac tttggctact agactctatc
     1681 aacagacaaa ttcattggta ttaactgccc aacaattggg acatacaagt acaaatacaa
     1741 caactttata tactcatata gacaatgcag ctacaattga tgctttgaac gctttgtaaa
     1801 tgtagtaaaa aatagggttc tgttgcaaag ttttaaataa agaataaaat cccttacggt
     1861 atctatgatt taagctggga ttcccaataa taccttgatt tcagtacaga ccgaaaaccc
     1921 gaagagagtg ccttcttttc gggttttctt atataatcct cggatggctt ccatgccttt
     1981 aat
//
LOCUS       DANKMN010000158         1859 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000158 DANKMN010000000
VERSION     DANKMN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="PW224_002907"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="PW224_002907"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2909519.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DANKMN010000159         1844 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000159 DANKMN010000000
VERSION     DANKMN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            469..1641
                     /locus_tag="PW224_002908"
     CDS             469..1641
                     /locus_tag="PW224_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDL2909520.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          644 a          341 c          301 g          558 t
ORIGIN      
        1 aaaaaagttc aaaagatcaa gtgtgcgctt cgctcccact cccctcgacg ttttagtagc
       61 ctttccctca cttcgttcag tccaagccaa ctaaaaagtt ttcgggatac attctccttc
      121 tcttcctaat aatcaattaa aattttaatc tttatatttc tgctaatcac tcaggaaaca
      181 gtacagaaaa acaaacacgt atcatagata taaatgtaat ggcatagtgc gggttttatt
      241 ttcagcctgt atcatagcta aacaaatcga gttgtgtgtc cgtttttggg cgttctgcta
      301 gcttgtttaa agtctcttga atgaatgtat gttctaagtc aaaaaactct gacagcgcct
      361 ttatatagat aaagtccgta taattgtgta aaagtaaaaa ggccatataa cagtcctttt
      421 acggtacaat gtttttaacg acaaaaacat acccaggagg acttttacat gacccaagta
      481 cattttacac tgaaaagcga agagattcaa agcattattg aatattctgt aaaggatgac
      541 gtttctaaaa atattttaac aacggtattt aatcaactaa tggaaaatca acgaacagaa
      601 tatattcaag caaaagaata tgaacgaaca gaaaaccgac aaagtcaacg aaatggctat
      661 tatgagcgca gctttacgac acgtgtaggc acgctagaat taaaagtacc cagaacacgt
      721 gatggccatt tttcacccac agtgtttgaa cgttatcaac gaaacgaaaa agccctcatg
      781 gcttcaatgt tggaaatgta tgtatcaggc gtttcaactc gtaaagtatc aaaaattgtg
      841 gaagaacttt gtggtaaatc cgtctctaag tccttcgttt ctagcttaac agaacagcta
      901 gaacctatgg ttaacgagtg gcagaatcgt ttattatcag aaaaaaatta tccttactta
      961 atgaccgatg tactctatat aaaagtacga gaagaaaatc gagtactctc aaaaagctgt
     1021 catatagcga ttggaataac caaagatggc gaccgtgaaa ttatcggctt catgattcaa
     1081 agtggcgaaa gcgaagagac ctggacaaca ttttttgaat acctaaaaga acgcggttta
     1141 caaggtacgg aactcgttat ttctgatgcg cacaaaggat tagtctctgc cattagaaaa
     1201 tccttcacca acgtaagttg gcaaagatgc caagttcact tcctaagaaa tatctttacc
     1261 accattccta aaaaaaattc aaaatctttc agagaagctg ttaaaggaat ttttaagttc
     1321 acagatatta acttagcgcg tgaggctaaa aatcgattga ttcatgatta tatcgatcaa
     1381 ccaaaatatt caaaagcttg cgcatcattg gatgatggat tcgaagacgc ctttcaatat
     1441 accgtacaag gaaattccca caatcgacta aagagtacca atctaattga acgactgaat
     1501 caagaagtac gcagaagaga aaagattatt cgcatcttcc ccaatcaaac atcagccaat
     1561 cgcttaattg gagccgttct tatggaccta catgatgaat ggatttattc ttcaagaaaa
     1621 tacatcaatt ttgataagta gaaatggtaa aaacattgta tagcatttta cacaggagtc
     1681 tggacttgac tcacttcctt tattattttt cattttttta aactatttaa tactaatgtc
     1741 ttttataata gcttttcata tattatataa tcaatcttta taagtccttt tataaatttc
     1801 ttttctacca ttttcgataa attcctgttt aatattttta attc
//
LOCUS       DANKMN010000160         1805 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000160 DANKMN010000000
VERSION     DANKMN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            4..963
                     /locus_tag="PW224_002909"
     CDS             4..963
                     /locus_tag="PW224_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2909521.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="PW224_002910"
     tRNA            1152..1225
                     /locus_tag="PW224_002910"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
BASE COUNT          598 a          315 c          340 g          552 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
     1321 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata
     1381 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttatat tgtgagataa
     1441 taataaagaa gagtttaaag cgttccccaa aaaccactgc cccataagtg tgttacgctt
     1501 taaactcttt tatatttgaa gccattaaaa agcataccat atttttgaaa aaaagtgaga
     1561 aaaaaggctt ataattggag tggtagttaa ttagtgactt atttttgatt ttatagcact
     1621 gatactataa aatatagata tcatcatatt acacaatctt aatactaact taaaaaatat
     1681 ctcctttcat aagtatggtg ataaaatccg ttccgggcta cctttttagg tagcctactt
     1741 taatctttgt atctttctgg atcaacgaaa gtatacttta tatagtcata acgccgatga
     1801 tcgct
//
LOCUS       DANKMN010000161         1721 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000161 DANKMN010000000
VERSION     DANKMN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            64..972
                     /locus_tag="PW224_002911"
     CDS             64..972
                     /locus_tag="PW224_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2909522.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="PW224_002912"
     CDS             972..1592
                     /locus_tag="PW224_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909523.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          616 a          255 c          391 g          459 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac a
//
LOCUS       DANKMN010000162         1719 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000162 DANKMN010000000
VERSION     DANKMN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(129..521)
                     /locus_tag="PW224_002913"
     CDS             complement(129..521)
                     /locus_tag="PW224_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2909524.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            complement(505..1719)
                     /gene="aph(2'')-I"
                     /locus_tag="PW224_002914"
                     /pseudo
     CDS             complement(505..1719)
                     /gene="aph(2'')-I"
                     /locus_tag="PW224_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
BASE COUNT          587 a          297 c          131 g          704 t
ORIGIN      
        1 aatgtatatt ttttatctct accatcataa aattctaata ctctttcatc agttaaccat
       61 tttaacatca aaggaaaatc atcatctatt aaagttctta tacatatttc attttcaact
      121 atattcattt atttatcacc tttttcataa tcatatacat atactatttc atctttataa
      181 tcatttttac cacctaattt ttcatataca tggcaagctc taggattacc tttatcagtt
      241 attaaaaaca tttcagaaca accaatctct ttagaatatt ccttaataaa agataataat
      301 tttgaaccat aacctttgtc ttgatagtta ggtaacattc ctattgagtg taaataaaac
      361 attgtttttc catcaggtct taaaagtgta tagcaatatg caaatcctat aattttatta
      421 ttttctttag ctataaaccc aaatgaactg gtatcattaa gaaatccttt taaattatca
      481 atatcaaaaa ccatattatc atcaataaac aatagtttca ataggataat attcttcaac
      541 tatatcttga tattcttttg ctttctcaat atctatattt ccatacattc ttaatatatc
      601 ttctccaaaa tttgttccta tttcttcttc actatcttca agtaagtata taaaatcaca
      661 atattcatct ataattccag aatctccaaa atcaattatt ccagttaatc tattattgcc
      721 atctaacaat agatgattac aactaaaatc attatggcat aaacactttt taccctcaaa
      781 aactgttgtt gcatttagtc tttccataaa actttctata taatcttttt ctatatcagt
      841 taaatcatta taaatagttt cacgcaacaa tatatactct tctaatacat tttgtttatt
      901 atcaatagta cattcactaa tatctgtata atctaaaccg tgcatttgtc ttaaaaaact
      961 ggcaatatct cgttttaaca aattttgttc ttcttctgac atagtagaat aaatttctgg
     1021 tgttaaaaaa gttcctttaa tttctttata acctagtata gataattcat cactaatata
     1081 cgaatattca atattaggaa tttttacatt agtttctaaa tttgtattta aaaaattata
     1141 tattgctttt tcttttgcat aacctttttt cttattagta ctaaattttg ttttaaaaat
     1201 gtattcatta ttaactaaat atgccacact atcataacca ctaccgatta tttcaatact
     1261 atctactttg aaattatcaa agtaatgctc aattaaatat ttcattgcct taacatttgt
     1321 ggcattatca tcatatctat attccattaa ataacaatct tcttttttgc cctcgtgtaa
     1381 ttcatgttct ggcaaatctt caataattct aaaaccagat ttttggtatg cccttattgc
     1441 tcttggatta tttttatgag ggtctaaaat aactgcatta gcatttcttt cttttttcaa
     1501 aaattcaaaa atcaatttaa tatatcttgt accaattcct ttactccaat aatttggctc
     1561 tcctataaat tgatccatac catagactat ctcatcagtt tttggataat gataatcagt
     1621 atataactca tcatacattt tatatatttg tccatatcca ataggaacat tgttatattc
     1681 aataattact ctaaaaactt catcttccca aggctctgt
//
LOCUS       DANKMN010000163         1677 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000163 DANKMN010000000
VERSION     DANKMN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            64..972
                     /locus_tag="PW224_002915"
     CDS             64..972
                     /locus_tag="PW224_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2909525.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1589
                     /locus_tag="PW224_002916"
     CDS             972..1589
                     /locus_tag="PW224_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909526.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          620 a          226 c          348 g          483 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt taatcagtca tatgtagatc
     1501 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
     1561 ttacttttga aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa
     1621 gcaatgtaca ggtttatcca caaactcatt ggaatgctat agaaggcaaa ccaacaa
//
LOCUS       DANKMN010000164         1526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000164 DANKMN010000000
VERSION     DANKMN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..734)
                     /locus_tag="PW224_002917"
     CDS             complement(<1..734)
                     /locus_tag="PW224_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2909527.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWH"
     gene            complement(731..1150)
                     /locus_tag="PW224_002918"
     CDS             complement(731..1150)
                     /locus_tag="PW224_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2909528.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(1220..1360)
                     /locus_tag="PW224_002919"
     CDS             complement(1220..1360)
                     /locus_tag="PW224_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2909529.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(1353..>1526)
                     /locus_tag="PW224_002920"
     CDS             complement(1353..>1526)
                     /locus_tag="PW224_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909530.1"
                     /translation="SGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
BASE COUNT          417 a          327 c          236 g          546 t
ORIGIN      
        1 atgtgccatc cagcgacgtg taagttcgca ttttcgattt ctccaaactg atcaatgtgg
       61 gcgtaatttg ttccatctgt cagtgtagga tttgctgcac ctgctctagt tggatcaatg
      121 actggtttat catctgaagt tgtcggattt tcatcggtaa atccatgagc taaatcatat
      181 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      241 ccccagatat tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggcgtt
      301 tctaatgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      361 ttgtagttct tttcaaacgt tactttatcg tgcgtatgtt gcagttcgat ttgcacagga
      421 ctatttgcat tagcatatga accagcgcca tattgtacat aaccaggttg tccaacttgg
      481 taaacgatcc caccatcgcc aacaatatac gctgtatagg cattgtacca agaacgtttc
      541 atgtactgcg cttcatttcg tcctgtcgca gtttcgtttg ctgtttcatg caataggatg
      601 tattggttat ttgctacttg agaactacct tcgtttgcgc ccaaattgaa ttcattgttg
      661 atcgtgtaag caaaggcgtt tattggcaat aaaaaaagag ctataatagc tcctacaaag
      721 attttctttt tcatttggtt cctcctcggt ctttattttc agtatcattt cgtatttgtt
      781 ggaatacctc ttttaactta tctggcaaag gaaggaatac cgaaatgttt tctaaaaaac
      841 taatcccttc atttgcaaca tagaacataa taactatttc tcttagaggt atagaattcc
      901 caacgatttt ctgtacttca accgcaacgg caatcacaac gaagattaag actttcttca
      961 acaatccttc gaaacctatc tcactactta tttctttcaa tttccatgcc ttagcaagtc
     1021 cagttaagaa gtccacaccc acaagaaaaa ttaaaacatg tagtatattg tccatccctc
     1081 ccaataaacc aacaattgtc ccgcctatca cccctgtcat tactgagaat gtatttaaat
     1141 atttttccaa tatctatttc tcctttatgc aaaaaagcgc actcgtttga gtacgctttt
     1201 tatttaactt tgtgcttaat taaatttcat ttccagtaat ctgcttatat tggtcttttg
     1261 ttaaacagtt catgtttaca tagtcaagta aatcttggtc cgtataacat ccccaatcat
     1321 aaaattgttt tatattttca aagcccggaa aactattcat tacttttccc tccattctta
     1381 atacttgcga cttccatcgt caattgagct aacatttgtt gcattttttc ttgttcctgt
     1441 accgatatcg ccaattgttt tgtcagtaga gcatttaatt gcttcaactg atcgacatct
     1501 tcaatatcgt caaccgctgt gccact
//
LOCUS       DANKMN010000165         1424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000165 DANKMN010000000
VERSION     DANKMN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(150..1337)
                     /locus_tag="PW224_002921"
     CDS             complement(150..1337)
                     /locus_tag="PW224_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL2909531.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DANKMN010000166         1397 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000166 DANKMN010000000
VERSION     DANKMN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            102..1397
                     /locus_tag="PW224_002922"
     CDS             102..1397
                     /locus_tag="PW224_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2909532.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          443 a          276 c          263 g          415 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      721 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      781 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      841 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      901 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      961 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
     1021 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1081 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
     1141 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
     1201 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
     1261 gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg
     1321 cgtacggctt tcgcaacttt tacaatttca aactacgaat tatgattcgt catggcaaaa
     1381 catttttaac caaataa
//
LOCUS       DANKMN010000167         1337 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000167 DANKMN010000000
VERSION     DANKMN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..1337
                     /locus_tag="PW224_002923"
     rRNA            <1..1337
                     /locus_tag="PW224_002923"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DANKMN010000168         1310 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000168 DANKMN010000000
VERSION     DANKMN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            127..>1310
                     /locus_tag="PW224_002924"
     CDS             127..>1310
                     /locus_tag="PW224_002924"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2909533.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LHT"
BASE COUNT          411 a          262 c          235 g          402 t
ORIGIN      
        1 tctataattt ctaggactga tggaaatttt ctatctgtcc tagttttttt gtaaaaaaac
       61 aaaaagacat acaaaatttc cggtagaata gagtctccta caacatcatt ctgggggaat
      121 tcttgtatgt ccatgaatca ttttatcaaa caactcttta ctattaaaga ctcaaatatc
      181 caaattcaat cctttgaaga aactttttat aaaagcagaa aaagtatcgt tattgaggga
      241 actctgatcc gtacctacag acgttgtccg tgttgtaaag attctacaaa gcaaatcgtt
      301 aaaaatggca agaagatctc tatgattctc ctgaatcgta gcggtaataa acggacctat
      361 cttcggttga agaaacagcg atacctctgc cgagcatgta aaaaatattt taccgctcgg
      421 acgtatctcg ttaccccatt ctgctttatt tctaagcaga ttcattacaa gatcttagaa
      481 gaattgacag agcgtcaatc tataaaagca attggtaaac attgcgatgt ttctgtgaca
      541 actgttcagc ggacattgtc cgccttgtca tcagatatcc gtcagaatct tgagtattta
      601 cctcggtgtc tattattcga tgagtttcgt tctctttcta catggcacgg taaattttct
      661 ttttcttgta tggacggtga gaccgggaaa ctgtttgaga tcttacctag ccgccgtaaa
      721 aaagatctcg ttgctttttt catgcgtttt tctctcaaag cgaggttggg tgtacattac
      781 atcgtaacgg atatgaacgc tccttatttc tcactagtta aagagtgttt tccaaatgca
      841 caagtgatcg tcgatcggtt tcacattgtt caacatctga accgcttctt cgatgcggtt
      901 cgaaaacgtg tgatgaaaac acttgatcaa aaagacccaa tacaagctaa acagtatcgc
      961 caattgaaat ccttattcaa attactttta aaatcagagg atcgtttaga ttacaataca
     1021 ctaacgaaaa gacgcaattt caattggaga ctattgactg aaacagaagt agttgaccgc
     1081 cttctaaaga tttctcctga acttaaaaca gcttatcgtt actaccaaga cctgcttact
     1141 gcgtatcagg aaaaagatcc agatactttt ttccagctgc ttcgagcaat gcctggagaa
     1201 gttccattgg aactgcacca tatcaaaaaa gcctttttca aatacgagaa agggattcgg
     1261 ctggcattac tgaagaagta ttcgaatgca cgactggaaa atctccatac
//
LOCUS       DANKMN010000169         1294 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000169 DANKMN010000000
VERSION     DANKMN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..1244
                     /locus_tag="PW224_002925"
     CDS             <1..1244
                     /locus_tag="PW224_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL2909534.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DANKMN010000170         1285 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000170 DANKMN010000000
VERSION     DANKMN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            35..532
                     /locus_tag="PW224_002926"
     CDS             35..532
                     /locus_tag="PW224_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2909535.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="PW224_002927"
     CDS             complement(569..1252)
                     /locus_tag="PW224_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909536.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          446 a          219 c          225 g          395 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaaatag tcctc
//
LOCUS       DANKMN010000171         1247 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000171 DANKMN010000000
VERSION     DANKMN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            200..742
                     /locus_tag="PW224_002928"
     CDS             200..742
                     /locus_tag="PW224_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909537.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1247
                     /locus_tag="PW224_002929"
     CDS             898..>1247
                     /locus_tag="PW224_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL2909538.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          515 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccaga
//
LOCUS       DANKMN010000172         1195 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000172 DANKMN010000000
VERSION     DANKMN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..1076
                     /locus_tag="PW224_002930"
     CDS             <1..1076
                     /locus_tag="PW224_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2909539.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DANKMN010000173         1174 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000173 DANKMN010000000
VERSION     DANKMN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(226..299)
                     /locus_tag="PW224_002931"
     tRNA            complement(226..299)
                     /locus_tag="PW224_002931"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(263..265),aa:Ile,seq:cat)
     gene            complement(854..1168)
                     /locus_tag="PW224_002932"
     CDS             complement(854..1168)
                     /locus_tag="PW224_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909540.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          443 a          186 c          172 g          373 t
ORIGIN      
        1 ttctttatta ttatctcaca atataaccca aatgtctttc catttaaaaa tcaaagtaaa
       61 acttttcaaa tatacagaag tataactatg tgaaacatcc tttcattaat ccataaaagg
      121 atacataaaa aagccactca tttgagtggc aatgaagaaa agctttagct tgtataatac
      181 tcttcaaaaa attctaacac agaacgattc aattggctac attaatgtac cctgtaggac
      241 tcgaacctac gaccggacgg ttatgagccg tctgctctga ccaactgagc taagggtagt
      301 ggttgttgcc acataaagcc ataaacaatc aaccagtaga atgcgtggca acaaacctgt
      361 tatcgcatat cttggagtgt gactatttat gagtgatagt gaagatatgc gtctccatat
      421 atgtatggtt gagacagata aacatgtatg aaaaacgttg ttaaatcaac gtttttttat
      481 tttggtcaag gagagaaaag tagagaaata caaaattttt tgcattgatt ttgcattcaa
      541 aaaaacattc aaacatttag aaatccccaa tacgtattca ggaaaatcta ttttaaggtt
      601 tacaaaaaga acttttgttc gtacactctt tttgaggtga acttttatgc tgatggaagg
      661 aaaaacacaa ttatggttta agcatgttcc ttcaaataag ttttttaatt ataaactcta
      721 gagaaattaa ttcatcctaa cataaaataa ttgttcactc aaaagttttt ctagaacaat
      781 taaaacagca ggcttcacat tcgctataca tttgtaagaa gtaataattc ctgtactaaa
      841 aggcataatt gctttaatta ttttttgact tctcttcaaa cacaagaagt agttgtgtaa
      901 taattgcaga aataaggatt ccaataatag atataaaatt tgacatggaa acgtctataa
      961 cattaaaaga ataaagtgtt gaagaaagta aactgattga aataacccct ccgtaaaaaa
     1021 tagtctgtga atatacccac tttttttcag aagacgtact tcgtttggtt ctaaaaccta
     1081 tccctgcatt tctactctta tcaaaaaacc aaatatttag aagtaataat attgctatta
     1141 ttaagataaa attactgtaa aaatccaaaa gcta
//
LOCUS       DANKMN010000174         1168 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000174 DANKMN010000000
VERSION     DANKMN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            26..286
                     /locus_tag="PW224_002933"
     CDS             26..286
                     /locus_tag="PW224_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909541.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(648..887)
                     /locus_tag="PW224_002934"
     CDS             complement(648..887)
                     /locus_tag="PW224_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909542.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          414 a          164 c          217 g          373 t
ORIGIN      
        1 gaaaaacatt aaggatggat gaaatatgaa taacgaaact atcattttag gaaaaaaata
       61 tatttgccaa cctattggct tgaaacaaaa agtggttgga gaagtaatca gtaaattaga
      121 aaattgtgtt gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg aaatattaga
      181 aaaatgcgga aaagtagtgg caaaatatac tcacgtttat gagttggaaa caacagaata
      241 tctatataac tatagcaaca aatatgaaca tactttagtt tcataaagat ttaaataaag
      301 tgccttcaaa agcgagaaga ccaagctttt gaaggcactt tatttagttg gcagtgattg
      361 ttcatgattt ttgatttatt caaaaggttc gcctagagaa tctgtctctg taacgaggcc
      421 tggtggagaa actcgataaa aacctatact gccagaactg tcctattttc ggatagacaa
      481 actagtagtt ttaaaaagat catccgatcc aatcatttca aaaaatgcaa aactgatatc
      541 aggatcattt agctcaggct tgttatcgta aagcatttgt aaaggttgca tttagttagg
      601 agtaaacgcg aaactatcat cattatttgc tgaagaagat gaagaagcta attcagagtt
      661 tgtttgaaat aaaggagacg tgctgacagt ttctttaccc tgttttttgc agaggtagaa
      721 acttttatat cggaagtttc taccggagaa tttttctcat ttgtagaaat tgttgaatcg
      781 atactattcg aaatttgcgt atctgaaggt atgttctttg atgcagaagt cccacaagca
      841 gtaagtaaca ataaatttga taaactgata aacagaattc ttttcattat gtacacactc
      901 cttatagtat cgtttagcat acacctatgc tcagtattag gcaaaatcaa tgcaaaacta
      961 tcaagtcttt tatagtatct attagtctta gtaatacata tatggagagg gtgatatccc
     1021 agttcaaacc agatacattg gttaccatct gtattaacga ttatgcctct aaacgctttt
     1081 tttatgaaaa aatttataag tgaaaacgta ggtatgatat tatctagagg taattttttt
     1141 gggtatttaa atagttgcta ggaggata
//
LOCUS       DANKMN010000175         1159 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000175 DANKMN010000000
VERSION     DANKMN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            14..1141
                     /locus_tag="PW224_002935"
     CDS             14..1141
                     /locus_tag="PW224_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2909543.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          418 a          200 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaa
//
LOCUS       DANKMN010000176         1108 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000176 DANKMN010000000
VERSION     DANKMN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            164..715
                     /locus_tag="PW224_002936"
     CDS             164..715
                     /locus_tag="PW224_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HDL2909544.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          416 a          158 c          212 g          322 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgaagagc gagcaagaca ggaacgagag aacaaacata ctcatgaaaa gactagaggc
      181 ttcgacctcg aactttaata ataagttaga tgtttttgaa gaaaagacta agcatgcaag
      241 attaaacttt gaaacaactg caaagcacta tattaaacga cttgatgaag ataatttgaa
      301 attagatttt caacaagcta ttcaagatga attaagcgat actaaagacg aaatacgtga
      361 agtgacgaaa caagcgagag aagaaacaaa agagtataaa gaaatcttag aaagtaaaat
      421 aaaagaccat aacaaagtgg ttgataaatc gaatacagcg ctaaaagtga tgacaaaagg
      481 cgttacaaat atattttttg tgctaattat ttttgtactt gtaatgcttg tgacaggtcc
      541 gataggtcat ttctttggta ttgaacattt atatagcttt attaatggtt ttattgatga
      601 ccacgaaagt gcatggcgat atttgatgtt gatactttat gcagtaccct atgtattctt
      661 cgctatcatt ctgtggctca tagcattgtt ttttagagta tttaatgacc tttaggtaga
      721 tttatatata aaaagggcta aatacgcaaa ataccccctt aaaatgcaaa ataaatgcaa
      781 ataagggggt aactccttat gaaaaaacaa ctaatagtat tataacacgt ttttatgccg
      841 agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga gttggttgga
      901 cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag ccctgtaggt
      961 tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa tgagtgagaa
     1021 aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc aatcattgat
     1081 agtttattat acttaccagc aatataaa
//
LOCUS       DANKMN010000177         1064 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000177 DANKMN010000000
VERSION     DANKMN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(39..995)
                     /locus_tag="PW224_002937"
     CDS             complement(39..995)
                     /locus_tag="PW224_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909545.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaa
//
LOCUS       DANKMN010000178         1061 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000178 DANKMN010000000
VERSION     DANKMN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            1..660
                     /locus_tag="PW224_002938"
                     /pseudo
     CDS             1..660
                     /locus_tag="PW224_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          402 a          160 c          163 g          336 t
ORIGIN      
        1 cggtatgaga acactttatc gtttattttc taaaggaata tttgatattg acacactacc
       61 gatgaaaggt aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac aacagtatca
      121 gcgctcaatc catgatcgac ctgataatta tcctcatttc aattctgagt ttggtcacct
      181 tgaaggtgat acgatcgttg gcattcatca taaaagtgcc gtcattactt tagttgaaag
      241 attatctaaa gtcattatca cgattaaacc caacggccgt aaggcattag atattgaaac
      301 tgcccttaat caatggtttt ctcgcttccc taaaaacttc tttaaatcta ttacgctatt
      361 acgtttgact gtggaaaaga attttctaac tggaaagcca ttagtaacca acatgatatt
      421 gatatatatt ttgcggaccc tggaacacct tctcaacgcc cattaaacga gaattctaac
      481 gggattctgc gtcgtaatgg actgccgaaa tcaatggatt ttagagaagt gaatcagaca
      541 tttatttcca gtgtcagcaa tcaacgtaat catattccaa gaaaatcatt gaattacaga
      601 acaccaatcg aaatattttt gagctatgta caagaagcat tttattctag cttaatttga
      661 caaatcataa ataataaaga attgaacttc tgagaaaaca aagaatcttt tttctttttt
      721 tgaattgact catggattgg cgttaaaatt ttattttgaa aaagttattt ctggtttaac
      781 tcaaaaaaag agatatgaat ctaatagtga tagaaaggat gttatttttc atgaaatggt
      841 tgttttagat ttaatacata taactaataa atgctattga atgtatttaa actaattaac
      901 aaaagaattt gaattgttaa taaaatccaa taaaatatat taataaagta gatagaaata
      961 aaaaaacaaa gactaaacat atataattgg attaaacgca atatctgcaa aatattgtaa
     1021 atttaaagcc tttgtagaaa aatttactga aagacaaact g
//
LOCUS       DANKMN010000179         1045 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000179 DANKMN010000000
VERSION     DANKMN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="PW224_002939"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="PW224_002939"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2909546.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DANKMN010000180         1035 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000180 DANKMN010000000
VERSION     DANKMN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..>1035)
                     /locus_tag="PW224_002940"
     rRNA            complement(<1..>1035)
                     /locus_tag="PW224_002940"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DANKMN010000181         1000 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000181 DANKMN010000000
VERSION     DANKMN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(82..636)
                     /locus_tag="PW224_002941"
     CDS             complement(82..636)
                     /locus_tag="PW224_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2909547.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          324 a          186 c          107 g          383 t
ORIGIN      
        1 acttccgaaa attcagttat cttcattaat ctcaccttct aagttttcat tatacacaaa
       61 aaatatatcc taatatctca attagcctcc caatttaaaa ccacgtaacg atgcccctat
      121 ttcatcttca gttataccaa tgtacctttt tgtaattgct tgcgagctat gattaaaaat
      181 aaacattaag gtagcaatgt ctctagtttt cttgtagtag tgatacccaa aagtttttct
      241 taatgtatgt gttcctatat catctcttcc taatttttct gctacatctg catatatctg
      301 atagacacga tcccttgtta tatgtcgacc ttgttgccga cttggaaata accaatcatt
      361 ttcttcttta ccttcagtat ataataaaat ctcttctctc aaattatcta taaaaatcgg
      421 ttttcgctta cctgttttat gttcaacaat aataggctta cttttacctc gtatgtctcc
      481 aacttttaat cctataatat ctgacatacg taacccatta ttgattccaa acaaaaataa
      541 aaaactatct ctttctggat tacttgttct tcttagccaa aactgaaaat cttgtatctc
      601 ttcatttgtc cttaacggtt gtacattata gctcactatt gtttcccttc ttaaaaatat
      661 tctgagttca tgattatata tatattttac cataatcatg aacttatata tatactctgt
      721 taaaagtgaa gaaaatcaag gggtttagtt tatgatttta tatcatttca gtaactcaga
      781 atattccgac ttttttgtac aaaaaaatta accttactta tatgaagatt ctaagctctt
      841 atttaaattt tagacgggca gttatccgta gaagttctta gaatagctaa tttacataat
      901 taaattatat gaaatttaag tttgaacaat atactaagaa ctccccttac tataaagtga
      961 aataaattag catatgaata accgtgctac caaacttttc
//
LOCUS       DANKMN010000182         1000 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000182 DANKMN010000000
VERSION     DANKMN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            369..1000
                     /locus_tag="PW224_002942"
                     /pseudo
     CDS             369..1000
                     /locus_tag="PW224_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DANKMN010000183          972 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000183 DANKMN010000000
VERSION     DANKMN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            78..>972
                     /locus_tag="PW224_002943"
     CDS             78..>972
                     /locus_tag="PW224_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL2909548.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHY"
BASE COUNT          330 a          182 c          192 g          268 t
ORIGIN      
        1 taggtattaa aaaacatatc gttctctagt atgattaaat caccacaaaa taaacaactg
       61 agaggacgat acgctatatg aatgattcta tcaaaaaaat gctgagaata acagagaaag
      121 atttgatgat tacagaggtc tcttacgaga cccttcagaa gaaaaagacg ttggtcgtcg
      181 atgctgttct ctcgcctact cctcgtgctt gtagaagttg tggttctact gtggtagatg
      241 gaaacgggaa agcaattata gtgaaaaatg gaaaaaagga aacgattgtc cgttttgaac
      301 aatacaatca tatgcctttg gttatgcgcc taaaaaagca acgctatacc tgtaaaaact
      361 gtcgcaccca ttggacggct caaagttatt ttgtccaacc cagacattca atcgcaaatc
      421 atgttagata taaaattgct tctttactga ctgaaaaagt atctttatct tttattgcga
      481 aaagctgtca ggtatctttg accaccgtta ttcgtacatt gaaagagttt aaaagctatt
      541 taccaaagca atctaagaag atcctcccca gagtattgat ggttgacgaa tttcgttcgc
      601 atgcttccat agaagataag atgagcttta tttgcgcgga tggcgaaaca ggaaaattaa
      661 tagatgtttt gcctacgcgt aaattacctc gattaacaag ctatttctta ggttgtacca
      721 atccagaaga agtagaattc ttggtgacag acatgaacgc cgcctacttc cagctcacca
      781 aacgtgttct gccaaatgcg aaagtcgtga ttgatcggtt tcatattgtc aaacacatga
      841 atcaagcgtt caatgagttg cgtatccgtg aaatgaatga acttcgtaaa gccggacaga
      901 aaagccaggc agaaaaactg aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca
      961 tcaaccatta tg
//
LOCUS       DANKMN010000184          943 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000184 DANKMN010000000
VERSION     DANKMN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..>943
                     /locus_tag="PW224_002944"
     rRNA            <1..>943
                     /locus_tag="PW224_002944"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DANKMN010000185          925 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000185 DANKMN010000000
VERSION     DANKMN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..582)
                     /locus_tag="PW224_002945"
     CDS             complement(<1..582)
                     /locus_tag="PW224_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909549.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYK"
     gene            complement(822..>925)
                     /locus_tag="PW224_002946"
     CDS             complement(822..>925)
                     /locus_tag="PW224_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2909550.1"
                     /translation="WTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          286 a          162 c          122 g          355 t
ORIGIN      
        1 tttgtaaaaa tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat
       61 gtcaatattt ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa
      121 ttccctgtaa ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc
      181 acccattcca ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc
      241 agtttgttta tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc
      301 aaaaattttt tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac
      361 tgataattga tactctatat tacatgactt aattagtttt ttagatcccc cctccagata
      421 ggcatatgtt tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc
      481 ataaattaaa aatgactgtc tatttagccc ataatctaat acaacttctt catactcata
      541 tttctttaaa tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt
      601 gttatttatt gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt
      661 agtgaactac tcaccactta gctaaaccta acggtttctt acgtttgaag tgggagcttc
      721 ttgggaagag agcatacttg atagcgtatt actttactaa cagaggtgcc tcttatttac
      781 caagctatcc ccgtagttcc tacggttctt gtttatttga tctaagccaa tcccatagag
      841 tgtagcctta gaccttcggt caggatattg atactagcgt tgatatctcg gtcatgatga
      901 gtatgacaaa tagggcaagt ccatt
//
LOCUS       DANKMN010000186          910 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000186 DANKMN010000000
VERSION     DANKMN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..888
                     /locus_tag="PW224_002947"
     CDS             <1..888
                     /locus_tag="PW224_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL2909551.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          389 a          120 c          149 g          252 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc
//
LOCUS       DANKMN010000187          864 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000187 DANKMN010000000
VERSION     DANKMN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..434)
                     /locus_tag="PW224_002948"
     CDS             complement(<1..434)
                     /locus_tag="PW224_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2909552.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="PW224_002949"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="PW224_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDL2909553.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          261 a          140 c          136 g          327 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atca
//
LOCUS       DANKMN010000188          855 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000188 DANKMN010000000
VERSION     DANKMN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            413..855
                     /locus_tag="PW224_002950"
                     /pseudo
     CDS             413..855
                     /locus_tag="PW224_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          306 a          148 c          156 g          245 t
ORIGIN      
        1 tatttatcac ggatgagctt gaaatcgtct tttatacgta tcaaattgaa atgttcggca
       61 tacaacctat cgttgttact acattactaa ttgtcacttg ttaaggaact tggatccttt
      121 ctaaacattc cctagagata aattttactt tatccctccg ttaatatcca atttaagcac
      181 ctttccgatt cagggttaat ttgcatatca agtgcagctt gtgttctagt aagattagcc
      241 cttttagtcc ggctgagttg acttatggat ataagatatg gtcatagcaa atagatacta
      301 cgtatagtct gttaatttag tgtcattaat cttaagttgg ataggctaca attaactaga
      361 cagaaaaatt aaggtgtgta gactagaaga aaacatacca ggaggaattt ttatgtctaa
      421 gagaacacga agaacttttt cacaagaatt caagcaacaa atcgtcaatc tttacttagc
      481 tggaaagcca cgtgtagaaa tcattcgaga atatgaacta acggcttcag catttgacaa
      541 atgggtaaag caatctaaaa cgagtggttc attcaaagaa aaagataatc ctacgcctga
      601 acaaaaagaa ttgttagaac tacgtaaaag aaaccagcaa ttagaaatgg aaaatgatat
      661 tttaaagcaa gcagcgctga tattcggacg aagagacaag taatcgatgc gaataagcat
      721 ctttacccta tatcagcgat gtgcagaata ttaggtctat cacgtcagtc ctattattat
      781 caatcaaaac caaagaaaga cgaatcagaa cttgaagaag cagtcgctga agaatttatc
      841 cgcagccgaa aggcc
//
LOCUS       DANKMN010000189          806 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000189 DANKMN010000000
VERSION     DANKMN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..693
                     /locus_tag="PW224_002951"
     CDS             <1..693
                     /locus_tag="PW224_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2909554.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          296 a          127 c          148 g          235 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct cccact
//
LOCUS       DANKMN010000190          800 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000190 DANKMN010000000
VERSION     DANKMN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(324..524)
                     /locus_tag="PW224_002952"
     CDS             complement(324..524)
                     /locus_tag="PW224_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2909555.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          287 a          125 c          127 g          261 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc
      781 ctttattcta tgctttatat
//
LOCUS       DANKMN010000191          792 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000191 DANKMN010000000
VERSION     DANKMN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            1..717
                     /locus_tag="PW224_002953"
     CDS             1..717
                     /locus_tag="PW224_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2909556.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          200 t
ORIGIN      
        1 ttgaaaaaaa ggattttagt aacaattggt ctagcttttt tgttagctgg ttgtaccaac
       61 gggaatgaat cgaaaaaaga atcaacaaaa gaaacagcga aagcaacagt tgcaactact
      121 acaacaacta gcacagagag caagtcaact gcagcaagta cagaaacagc agatacctct
      181 acttcgtcag agtcttccaa aacgactgaa acaactcaaa catctacttc aagttcttct
      241 gtagaggctt catcttcctc ttcagaaagc agcgaacaag ttgaacaaac actgtggaat
      301 gcagacaaat ccgcacaatt agaggcattt atgaccaaat ggggccaaac aatgggacaa
      361 caatacaaaa gttatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa
      421 gctattttga acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg
      481 tcagaaagtg ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag
      541 acagaaccat atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa
      601 gtattggtga cacaacaaaa tcaggggaat cccgataatt atctttactt caacgagaca
      661 gcgaataatg aattgaaaaa tggctttaat caaatcgttt atggacaagc aaattaatga
      721 gataaacaag gggttgtgac atgtctttta tcacaacccc ttgctcagtt tatttgttag
      781 tcttttattt tt
//
LOCUS       DANKMN010000192          765 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000192 DANKMN010000000
VERSION     DANKMN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            660..>765
                     /locus_tag="PW224_002954"
     rRNA            660..>765
                     /locus_tag="PW224_002954"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          315 a          124 c          134 g          192 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgtgcctaa tacatgcaag
      721 tcgaacgctt ctttttccac cggagcttgc tccaccggaa aaaga
//
LOCUS       DANKMN010000193          731 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000193 DANKMN010000000
VERSION     DANKMN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="PW224_002955"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="PW224_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL2909557.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="PW224_002956"
     CDS             complement(481..675)
                     /locus_tag="PW224_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909558.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DANKMN010000194          698 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000194 DANKMN010000000
VERSION     DANKMN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
BASE COUNT          267 a           87 c           86 g          258 t
ORIGIN      
        1 tattaatatt ttataacttt acgcaagatc ataattttgt cgtgtagata tttcattata
       61 tcactaaaga taataagatt taatgacctc attaatattg tttgctaggt ccaaactctt
      121 attaaacttt ctttgctata aaacccaaac atagtaataa aagaataact tattaccttt
      181 aaattatact accaacaaat ggtagtatat tattaattta tataatatga aacctaaaat
      241 atagtagtat gttaatttat actaaattaa ataaagtact acctttaata ggtagtactt
      301 tattatcaag ccattattcc ttcttctact agttcttttt gtcacataat tttgcaatca
      361 ataaatgttg cgcaataagt ataagaattc cattttctaa atttgaaagg tgatccataa
      421 tttcatgttc aagtagttta ttaattgtcc acttgtcgct tcatctaaaa attttatagg
      481 ttttaggaat gctattcgct tattttagga agattttaac tgtttgctgt agatatatat
      541 catactagta tcgtaataaa agtactctaa aagaagaatt ttcaaatacg caaaattaag
      601 tacgaaagag ttgaaacaaa tttggagaaa aatgagcata gaatgtctaa tgagttaaat
      661 agacctaaaa attatctaaa agtggagcaa aattagga
//
LOCUS       DANKMN010000195          692 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000195 DANKMN010000000
VERSION     DANKMN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            94..>692
                     /locus_tag="PW224_002957"
     CDS             94..>692
                     /locus_tag="PW224_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2909559.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWH"
BASE COUNT          233 a          125 c          145 g          189 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac at
//
LOCUS       DANKMN010000196          571 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000196 DANKMN010000000
VERSION     DANKMN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
BASE COUNT          186 a           81 c           81 g          223 t
ORIGIN      
        1 tcttgatgtg atatccttgt cttttttgta atgtttttaa cttattgctt ttgcaaaatt
       61 tatttacctg ttttctggat caacgatagt gtacattatg ctatccttgt ttggatttta
      121 ggacgtttaa agaggcactt ttttacataa cttaatccat agacctttta agcatgctgt
      181 gagataataa taaagaagag tttaaagcgc accccaaacc acttccccat aagtgtatta
      241 cgctttaaac tcttttatat ttgaattcac taagaagcat accatatttt tgaaaaaaag
      301 tgagaaaaaa ggcttataat taggatttag agtaattaat tagatgttta ttttatagga
      361 ctgatactat agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg
      421 tgaataagat aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac
      481 ctttctggat caacgaaagt atactttata tagtcataac gccgatggta actatgcact
      541 gtaacgataa tgctgctaaa atatcttttt a
//
LOCUS       DANKMN010000197          567 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000197 DANKMN010000000
VERSION     DANKMN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            141..398
                     /locus_tag="PW224_002958"
     CDS             141..398
                     /locus_tag="PW224_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909560.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          238 a           69 c           81 g          179 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtc
//
LOCUS       DANKMN010000198          555 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000198 DANKMN010000000
VERSION     DANKMN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..>555
                     /locus_tag="PW224_002959"
     CDS             <1..>555
                     /locus_tag="PW224_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2909561.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCG"
BASE COUNT          204 a           87 c           97 g          167 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt gtgga
//
LOCUS       DANKMN010000199          542 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000199 DANKMN010000000
VERSION     DANKMN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            210..>542
                     /locus_tag="PW224_002960"
     CDS             210..>542
                     /locus_tag="PW224_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2909562.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          189 a           85 c          106 g          162 t
ORIGIN      
        1 tacttcttgt atcattgatt atttcttcat tcaatatatt aagaaggaat ccaaaagaat
       61 tattaattga caataattaa aattttcggt gctttttagc accaaaaatg agagtgtaat
      121 ataaaccgtg taagtaacca tcaatcctag gcgggtggtt gcttcacggt ttttattttt
      181 ctaaaataag atcaagaggt gattacacga tggctagaaa gaaaagaaat cctgatgctg
      241 agaagttagc tgaatccatt ttgaatactt atcaacccga atctgtagag gatatgcaag
      301 atgttttaaa agatgtgttt ggtcccttat ttgaaaaaat gcttcaagga gaattgaata
      361 accatttagg gtatgacgcc cattctaaag agccaaagaa acatgacaat cgacgaaacg
      421 gttatggcaa taagacgctt aaaacgagct ttggtaaagt aaacattgat gttcccagag
      481 accgggaggc ttcttttgaa cctgagttga ttcctaatag aaaacgagat gtctccgaca
      541 tc
//
LOCUS       DANKMN010000200          534 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000200 DANKMN010000000
VERSION     DANKMN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..>534
                     /locus_tag="PW224_002961"
     CDS             <1..>534
                     /locus_tag="PW224_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2909563.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DANKMN010000201          529 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000201 DANKMN010000000
VERSION     DANKMN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            33..>529
                     /locus_tag="PW224_002962"
     CDS             33..>529
                     /locus_tag="PW224_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDL2909564.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          194 a           98 c           87 g          150 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctactta
//
LOCUS       DANKMN010000202          528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000202 DANKMN010000000
VERSION     DANKMN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(5..90)
                     /locus_tag="PW224_002963"
     tRNA            complement(5..90)
                     /locus_tag="PW224_002963"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="PW224_002964"
     tRNA            complement(107..178)
                     /locus_tag="PW224_002964"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="PW224_002965"
     tRNA            complement(190..262)
                     /locus_tag="PW224_002965"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="PW224_002966"
     tRNA            complement(280..361)
                     /locus_tag="PW224_002966"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="PW224_002967"
     tRNA            complement(369..441)
                     /locus_tag="PW224_002967"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="PW224_002968"
     tRNA            complement(445..517)
                     /locus_tag="PW224_002968"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DANKMN010000203          514 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000203 DANKMN010000000
VERSION     DANKMN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(107..>514)
                     /locus_tag="PW224_002969"
     CDS             complement(107..>514)
                     /locus_tag="PW224_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDL2909565.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DANKMN010000204          475 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000204 DANKMN010000000
VERSION     DANKMN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..258
                     /locus_tag="PW224_002970"
     CDS             <1..258
                     /locus_tag="PW224_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909566.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            260..397
                     /locus_tag="PW224_002971"
     CDS             260..397
                     /locus_tag="PW224_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2909567.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
BASE COUNT          184 a           61 c          105 g          125 t
ORIGIN      
        1 aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg
       61 ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta
      121 gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat
      181 tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa
      241 ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg
      301 ctgtttcact aacgaacaag taatggtttt cgttccgttg tgcattactg aagaagaagc
      361 agataaaatc attagcaaag aagagagcgc atcttaattg atgtgctttt tattttgatt
      421 caaggagttg tcacatgatt aatttagggg aatggggagc gatagcagga tcaat
//
LOCUS       DANKMN010000205          473 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000205 DANKMN010000000
VERSION     DANKMN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(346..>473)
                     /locus_tag="PW224_002972"
     CDS             complement(346..>473)
                     /locus_tag="PW224_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HDL2909568.1"
                     /translation="YKISGGGCYLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT          153 a           66 c           62 g          192 t
ORIGIN      
        1 ttatttagac caacagggta taccctgttg gtctttttgt gcgttcatga tatacttggt
       61 gtcattttta tatttaaatg gcacaaatat ttctaattat ttgtcattta acgctgaaaa
      121 tttttgctat ttatctaaaa aagtgacagg atactcattt ggccttcata gaattttcta
      181 aatatgaatc atcaatgaat ttaattaaaa aaataagtag ggcaaaatat aaatgttttt
      241 gccttacttg tttccatcag tttagtttta aattgataag ttatatagtt atggttgaat
      301 gtgtgtctcg catattagta tttttaccat ctgctagttt tattattaat ctataacaat
      361 agataattta taattcgcat attcattaag ctgttccaaa aagctgttca ttgatttttt
      421 tgaagtaaat ctacattcca aaagataaca gccccctcca ctaatcttat aat
//
LOCUS       DANKMN010000206          470 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000206 DANKMN010000000
VERSION     DANKMN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..>470
                     /locus_tag="PW224_002973"
     CDS             <1..>470
                     /locus_tag="PW224_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HDL2909569.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT          167 a           78 c           88 g          137 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg
      421 acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta
//
LOCUS       DANKMN010000207          469 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000207 DANKMN010000000
VERSION     DANKMN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(46..>469)
                     /locus_tag="PW224_002974"
     CDS             complement(46..>469)
                     /locus_tag="PW224_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2909570.1"
                     /translation="DEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTA
                     LKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKE
                     NSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          167 a           71 c           24 g          207 t
ORIGIN      
        1 aataagtctc tatttttcaa taactattgc aaaattatat cttaattaag aaaatatttt
       61 tattttaaag ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt
      121 cagaatatta cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa
      181 tttaacatca aatattttat ccatattcga aattttattg ttaccgtatg aattctctat
      241 atataaccaa tcattaacta tcccaatatt aattacccca tttacatcag tatattttac
      301 cgcattacta atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt
      361 ctcgtctaat atataattat tgacagttat tttcttttga tttgctaata tttcatactt
      421 ttttaatatg tcttctaatg tatcatttat tttcaaatat tcttcatca
//
LOCUS       DANKMN010000208          462 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000208 DANKMN010000000
VERSION     DANKMN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..430)
                     /locus_tag="PW224_002975"
     CDS             complement(<1..430)
                     /locus_tag="PW224_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2909571.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DANKMN010000209          438 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000209 DANKMN010000000
VERSION     DANKMN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            77..427
                     /locus_tag="PW224_002976"
     CDS             77..427
                     /locus_tag="PW224_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2909572.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          180 a           47 c           71 g          140 t
ORIGIN      
        1 attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt tttggtagtt
       61 ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag tcatacatga
      121 attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag atattactga
      181 tgttttattt caggtatata aaaaaattga tacagcaaaa tatcctgaaa ttcttgtaaa
      241 taaattagtt aattatattt acatcgtggg ttttgataat aaaatacgct ttattgataa
      301 tgatgaaaaa ttattaatag aattagggga tatttctaaa aaagctggaa ttaatagtaa
      361 atacaaagca aactttacag ataaatcgca attttatagt tattcagaaa caatgcctca
      421 acgataaaat atagtata
//
LOCUS       DANKMN010000210          421 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000210 DANKMN010000000
VERSION     DANKMN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..390
                     /locus_tag="PW224_002977"
     CDS             <1..390
                     /locus_tag="PW224_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2909573.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          163 a           74 c           65 g          119 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 a
//
LOCUS       DANKMN010000211          385 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000211 DANKMN010000000
VERSION     DANKMN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            12..>385
                     /locus_tag="PW224_002978"
     CDS             12..>385
                     /locus_tag="PW224_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909574.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGS"
BASE COUNT          130 a           52 c           95 g          108 t
ORIGIN      
        1 gggacggttt aatggctgat attgtgcaat taaaagaaga cggagttgct aaatacttaa
       61 aaacgcatgt agaagctatt gatggtaaag aagcattggt gcagacagat ggagaccaag
      121 ccattgcagg acataagaat ttttcaggtt ctgtaacaat caataataaa cgtgttttga
      181 cgactgatga taacagatat gaagtggtaa atctggttgt cactaacggt aatacaggga
      241 cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa
      301 aaagtagtgg cgggaatgat tcggttattt taactgttcc agaaggctat cggacaccaa
      361 ttagttttga acaactggtt gggtc
//
LOCUS       DANKMN010000212          375 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000212 DANKMN010000000
VERSION     DANKMN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..132)
                     /locus_tag="PW224_002979"
     CDS             complement(<1..132)
                     /locus_tag="PW224_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2909575.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DANKMN010000213          367 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000213 DANKMN010000000
VERSION     DANKMN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..>367
                     /locus_tag="PW224_002980"
     CDS             <1..>367
                     /locus_tag="PW224_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2909576.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGER"
BASE COUNT          137 a           61 c           80 g           89 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgag
//
LOCUS       DANKMN010000214          321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000214 DANKMN010000000
VERSION     DANKMN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(69..321)
                     /locus_tag="PW224_002981"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="PW224_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DANKMN010000215          292 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000215 DANKMN010000000
VERSION     DANKMN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..185
                     /locus_tag="PW224_002982"
     CDS             <1..185
                     /locus_tag="PW224_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2909577.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DANKMN010000216          281 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000216 DANKMN010000000
VERSION     DANKMN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.227"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            28..>281
                     /locus_tag="PW224_002983"
     CDS             28..>281
                     /locus_tag="PW224_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2909578.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           84 a           53 c           56 g           88 t
ORIGIN      
        1 tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa acaatacgaa tggtagaaat
       61 caatttgcga tgcttacgat tgatgatcta gttcctcaag atcatctcgt tcgaaagatt
      121 gatgccgcgc ttgattttga atttatttat ccaatcgtcg aagccactta ctccgattta
      181 ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg tgtttattca gtatttattc
      241 ggtatccgtt ccatgcgaca aaccattaag gaagtcgata c
//
LOCUS       DANKMN010000217          280 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000217 DANKMN010000000
VERSION     DANKMN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            78..>280
                     /locus_tag="PW224_002984"
     CDS             78..>280
                     /locus_tag="PW224_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2909579.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKK"
BASE COUNT          102 a           40 c           61 g           77 t
ORIGIN      
        1 taggtattaa aaaacatatc gttctctagt atgattaaat cacgacaaaa taaacaactg
       61 agaggacgat atgctatatg aatgattcta tcaaaaaaat gctgcgatta atagagaaag
      121 atttgatgat tacagaggtc tcttacgaga cctttcagaa gaaaaagacg ttgatcgtcg
      181 atgctgtttt ctcacctgct ccccatacct gtagaaattg tggttctact gtggtagatg
      241 gaaacgggaa agtaattgta gtgaaaaatg ggaagaagga
//
LOCUS       DANKMN010000218          274 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000218 DANKMN010000000
VERSION     DANKMN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            93..>274
                     /locus_tag="PW224_002985"
     CDS             93..>274
                     /locus_tag="PW224_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2909580.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           57 c           44 g           89 t
ORIGIN      
        1 gcttctttta cctatgaaat attttcacgt gttaaagtat tcctcgttga acacaacaaa
       61 taaaactttt taacgaataa aggagatttt tcatgacatc acttaaaact ttactttttg
      121 gaacaacttt ggctgccggc gctgcattct tcatgggaac gactgctcat gcggacgaag
      181 cttataccgt tcaatcaggt gatactctat caacgatttc tcaaaaatac gttggtgata
      241 actccttgat caatgccatt gcagaatcaa attc
//
LOCUS       DANKMN010000219          266 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000219 DANKMN010000000
VERSION     DANKMN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(34..>266)
                     /locus_tag="PW224_002986"
     CDS             complement(34..>266)
                     /locus_tag="PW224_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2909581.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DANKMN010000220          264 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000220 DANKMN010000000
VERSION     DANKMN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.denovo.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            113..>264
                     /locus_tag="PW224_002987"
     CDS             113..>264
                     /locus_tag="PW224_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909582.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWV"
BASE COUNT           98 a           43 c           49 g           74 t
ORIGIN      
        1 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
       61 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      121 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      181 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      241 ttttgataaa aaacagtggg ttga
//
LOCUS       DANKMN010000221        16925 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.guided.065, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000221 DANKMN010000000
VERSION     DANKMN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.guided.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(<1..353)
                     /locus_tag="PW224_002988"
     CDS             complement(<1..353)
                     /locus_tag="PW224_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL2909583.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWT"
     gene            complement(397..2430)
                     /locus_tag="PW224_002989"
     CDS             complement(397..2430)
                     /locus_tag="PW224_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2909584.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(2464..3315)
                     /gene="rfbD"
                     /locus_tag="PW224_002990"
     CDS             complement(2464..3315)
                     /gene="rfbD"
                     /locus_tag="PW224_002990"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL2909585.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(3413..4441)
                     /gene="rfbB"
                     /locus_tag="PW224_002991"
     CDS             complement(3413..4441)
                     /gene="rfbB"
                     /locus_tag="PW224_002991"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL2909586.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(4463..5035)
                     /gene="rfbC"
                     /locus_tag="PW224_002992"
     CDS             complement(4463..5035)
                     /gene="rfbC"
                     /locus_tag="PW224_002992"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL2909587.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(5049..5915)
                     /gene="rfbA"
                     /locus_tag="PW224_002993"
     CDS             complement(5049..5915)
                     /gene="rfbA"
                     /locus_tag="PW224_002993"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL2909588.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(6015..6749)
                     /locus_tag="PW224_002994"
     CDS             complement(6015..6749)
                     /locus_tag="PW224_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2909589.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            complement(6727..7554)
                     /locus_tag="PW224_002995"
     CDS             complement(6727..7554)
                     /locus_tag="PW224_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2909590.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(7554..8354)
                     /locus_tag="PW224_002996"
     CDS             complement(7554..8354)
                     /locus_tag="PW224_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2909591.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(8347..9486)
                     /locus_tag="PW224_002997"
     CDS             complement(8347..9486)
                     /locus_tag="PW224_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL2909592.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(9691..10884)
                     /locus_tag="PW224_002998"
     CDS             complement(9691..10884)
                     /locus_tag="PW224_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2909593.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(10963..11169)
                     /locus_tag="PW224_002999"
     CDS             complement(10963..11169)
                     /locus_tag="PW224_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL2909594.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(11162..12304)
                     /locus_tag="PW224_003000"
     CDS             complement(11162..12304)
                     /locus_tag="PW224_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2909595.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            12816..13451
                     /locus_tag="PW224_003001"
     CDS             12816..13451
                     /locus_tag="PW224_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2909596.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            13793..14428
                     /locus_tag="PW224_003002"
     CDS             13793..14428
                     /locus_tag="PW224_003002"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2909597.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            14535..15773
                     /locus_tag="PW224_003003"
     CDS             14535..15773
                     /locus_tag="PW224_003003"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2909598.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            16000..16419
                     /locus_tag="PW224_003004"
     CDS             16000..16419
                     /locus_tag="PW224_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2909599.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            16424..16663
                     /locus_tag="PW224_003005"
     CDS             16424..16663
                     /locus_tag="PW224_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2909600.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         5160 a         3324 c         2982 g         5459 t
ORIGIN      
        1 tttgtccact cgatccattt aattactaaa caaaataaaa taatcaaagg aaacagtaag
       61 tctccaaata aagactcatt tcttaattcc cgaaagtaag aaaagaaaat gaaaagggca
      121 agcgcaacac ctagataaag aaaatcgacg ccttttgctt tataaacatt taaaatgagc
      181 aaaatagcca cactaaaagc gataagtaat gaactattgt tatatataaa caaagttgct
      241 ggaaaaccta accctgctgt ccaaacaaaa aagactagcg tcagtaacaa aagcaagaaa
      301 ccgacactca ttttgttgac aaatttttgt acgattgctt gaattatctc cactctcctt
      361 tctactaaca tttcaggatt attctcagct agcctattac tgtacgacta gctgattttc
      421 ctgagtttgt gtttgtggta catctggata atccggatat tctactttaa aaatcatata
      481 accgtcttta tccggtccat atagctgcgt gaactgcgct tcatataatt ggttgtaatc
      541 agttaatggt cgtttactga tcacatagtc gatccctaat ttttctgcgt cttcaaaatt
      601 caacgtgacc gaaaacatat ctggacgatc taaaatgaac tttgtatctt ccttagcgat
      661 aaaagctctt gtatgagcat accgattata atacttctcg tattcttctt tcggatcgat
      721 tttcttccat gcagctatat ctggataaaa gcgtactgta ttgaaggatt tcactcccaa
      781 tgttggggtg aagttatata attcatcttc agacaaccaa actttaccag gatcttgttg
      841 atcgattttt tggatttctt ttgccagtgt tttttcaaaa atcgctcctg taccttctac
      901 gactggattg acgaaaaagc cagatacgat aactaaggcg gcaaaagcga ggtaaaacaa
      961 gcgtctccag ccaaataatg caaaagctaa aatcactgtt cccaacacag caactgcgat
     1021 ggtttcaaaa tccgaaaaat aattatccat tttcgaatgc gtaatcgcat agaaataaga
     1081 ccatgcattt actgccaaca aaatgaattt ataccaaata ggaataacat cttctctcca
     1141 aatataagcg ataaaccaga tcgataataa acaagccgca aaactaaaag taaggatggc
     1201 tcttaatgga ggaacataag acagcagagt gatttctgct agccattttg gcaacccaac
     1261 ggtaatccaa aagatagaaa acagacaaaa aatcatcaaa atacgtccca gtaacttttg
     1321 ttcgctatct tttttgccaa ataacacaaa tggacttgct aggaatacag ttggaaaaaa
     1381 gtgatagaac agggccactt cagagttatt cgaatagttg atatcttcaa aaggaatttt
     1441 ccaatttgta aggaaagaaa aaaattcgcc aatcgtccac tttcctccag tacttacccg
     1501 ttttcctgga tataaggtat tcaaagaagc taacaatgcg tcttttgacg ttagccagaa
     1561 atgaaccatg actccgccaa taaataaaac tgcccctcca atcaacacta catccaacag
     1621 atcccatgaa actttcttgc tatagtaaat aagcaggcca acaaaataaa aaatcagcaa
     1681 gtacgccagc atcacttgat gggctgggta aatcactaaa ataaacccaa taccgaaaat
     1741 aaccaccaaa agcatcatca acgttcgtag ccatttttta tcatgattgt agaaataatg
     1801 ataaaaagca gtcatcagtc ccaaagtaaa gaaaatcaaa tcgcccacat gttgcatgaa
     1861 ccaccactgg actgctggag aaaatgtcag cgcaaatgca ccgattagcg atagagcttt
     1921 ttttctcttc gttaatatca tcagtacttc gaatccaagc aagaccatga cgactaattt
     1981 catcgcccaa taaaaggaaa gtcctcgatc tcttccgagg aaaaagaatc cccagttgaa
     2041 tggttttccg ataaccgtaa tatcctttac aggagaatta tacgcaatga tcatgttttg
     2101 tccgtttaat gaataatcct cgttcaccaa cggaaagtcc tcttcagctt gtgagaggta
     2161 aaaaggtgtc tgcaccagcc attcgtctga ccgcacttcc cgattctgtc caaaaatcac
     2221 atcagttttt ttcccatcgt ctctttgcga cacatattta tcccaaacac caagtgaact
     2281 tccgtttaaa ttaaaagtaa cgatgaccac gatgactaaa acgccaataa gatagcggag
     2341 ttttagaaac aggtcaagca ccaaattcga atctgcctgg ttcgcttttg gcttttcttt
     2401 tcgtcgtgca cttcttgttt gtttttccat atcatgctcc aaattctatt tgataaaagg
     2461 aaattactta ttcatttttt tggcagcttc cagcatttgc gcaagtgctt cttgccaagt
     2521 tggtatgaca aatcctaact cttctgtctt tttcaggctc atgactgaat actgcggacg
     2581 tgttgctttt tgcggaaatt cttctgacgt aacaggtgca acttctacat ctgtatcttt
     2641 caatatttct tttgcgaatt cataccaaga acagctattt tcgttcgaca aatgataaat
     2701 accaaatggt gctttttctt cgatgacata taccataaat tctgccaaag tacgtgtcca
     2761 agttggtcgc ccaaactggt catttactac agtcagtttg tcgcgggttt ccgctaggcg
     2821 ttgcatcgtg aagacgaaat tgtgtccata gatcccgaaa acccaagaag ttcgtatgat
     2881 ataatagtct tctaaaatct cttctactgc tttttcgcct aataatttcg tacgaccata
     2941 ttcatttagt ggattcgttt ggtcgtctac ttcgtattct acatttttgt tttttccatc
     3001 gaaaacatag tctgtactga tatagacgaa ttgagcattc acagcttttg ctgcaagtgc
     3061 tacatttctt gtaccatcaa cattgatctt ctcatctaat tcttttgctt cttcttctgc
     3121 tttatctact gctgtgtaag ctgcacaatg gaaaataaca tcaggtttta attctgtgat
     3181 gtaagccatt gttttctcgg catctgtgat atccaagtct tttgcatctg ttgaaacata
     3241 ttgcagattt ttttcatcta ataaatggcg caattctgta cctagttgcc catttcctcc
     3301 agttaataaa aacatatgat tatccgctcc ttgattttta ttttaaaaaa gagacaggcg
     3361 agcgcctgcc tcacatgtgt ttttccatta atacagtgac attttctctg tattattgtc
     3421 catttttggc atatgctgct tcaacggcct ctttttcttt tttccaccag tcttcatgtt
     3481 cttcgtacca tttgatcgtg tcagccaacc catcacggaa gttcgtaaac tcaggcttcc
     3541 atcctaactc ttcccgtaat tttgtcgaat cgattgcata acgcaagtcg tgtcctgcac
     3601 ggtcgtttac atggtcgtac gcatcttttg gttgacccat catttctaag atcaattcca
     3661 tcacggtctt attatcttct tcaccgtctg caccgatcag ataggtttcg ccaattctgc
     3721 ctttcgtcaa aatcagccac actgctgaag aatgatcatt cgtatggatc cagtcacgaa
     3781 cattctttcc ttcgccatat aattttggac gaataccgct taagatattc gttacttggc
     3841 gaggaataaa tttttcaata tgctgataag gaccatagtt gtttgaacag tttgaaatgg
     3901 ttgcacgtaa gccaaaagaa cgcacccatg cttttactaa cagatctgaa ccagcttttg
     3961 tagaagaata tggacttgaa ggattatatg gtgtttccgc agtgaatttt tctccttcgc
     4021 cttccccatg tcctggcagg tcttcacgta atggaagatc gccgtaaacc tcgtcagtag
     4081 atacgtgatg atagcgtaca tcatacttgc ggcaagcttc taaaagtgta tacgtaccaa
     4141 tgatattcgt ttgtacaaat gggaaaggat cttttaatga attatcgtta tgggattctg
     4201 ccgcgtaatg aacaactgcg tccgcttctt ttactagacg gtccactaat tctgcatctg
     4261 caacatctcc tacgaccaat tctacgcgat cgctaggaag tccagccaag ttctctttgt
     4321 ttcctgcata agtcagttta tccaaaacag tgacatgcac ttctggatga ttgtttacca
     4381 cgtaatgaac gaaattagag ccgatgaatc cggcacctcc ggtaacaatg atgtttttca
     4441 taagaaaatt tctccttaat tcttagattt ctccgtaaac aaatggattt tctgcttcaa
     4501 attcttttaa tgtcggatga cggtgatctt tttccgacat aatcgctctt gatggatcaa
     4561 ttggccaatc aatcgctaaa tctggatcat caaatgcgat gcccccatct gcttctgcat
     4621 tatagtaatt atcacactta taaaggaagt tcacatttgg tgtcaaagta acgaatccat
     4681 gggcatatcc tttaggtaca agcaactgtc gatgattatg ttcagataag ataaatccgt
     4741 cccattgacc ataagtcggg ctacctttgc ggatatccac tactacatct aaaactgctc
     4801 cagtgactac ccgaatcaat tttgtctgtg cagcttctcc tttttggaaa tgaagccccc
     4861 gaaggacacc agcttcagct gatagtgaat ggttgtcttg aacaaagtca tatttcaatc
     4921 catgttctgc aaattttgct tttgaataac tttctgtgaa gaaaccgcga tgatcgccaa
     4981 agacgtccat ttctacgatt ttgacatctg ttaattttgt atctgttact ttcatctgta
     5041 aggactcctt attcagcggc caaattcaat aaatattgac cgtattgatt ttttttcaat
     5101 ggctgtgcaa gggccagcaa ttgttcctta tcgatgtagc ccatacggta agcaatttct
     5161 tccaaacaag ccacttttaa gttctgacgt ttttcaattg tttcgataaa ggtagatgct
     5221 tcaagcaatg attcatgtgt tcctgtatca agccaagcaa atccgcgtcc cataagttct
     5281 acggatagtt ttcctttatc caaatatgct ttattgacat ccgtaatctc tagttctcca
     5341 cgttctgaag gtttgatacc tttagcgata gaaattacct cattgtcata aaaataaagg
     5401 ccagttacag cgtaatttga ttttggctga gcaggctttt cttcaatcga caaagcttgc
     5461 atattctcat caaattctac tacaccaaag cgttctggat cattgacatg atagccaaat
     5521 actgtcgcgc ctgtatcttt tgaagctgct cgttgcaaca ttttagacag accgccacca
     5581 tagtaaatat tgtcccctaa taccaagcag acactatcat ctccaataaa ttcttctcca
     5641 atgataaacg cttgtgccaa tccatctgga ctttcttgta ctgcatattc gatctgcagt
     5701 cctaaatcat ttccgtttcc aaataattcc ttgaaacgtg gtgtatcttg tggcgttgaa
     5761 ataatcagaa tttctttgat tcccgccaac atcagagtag acattggata ataaatcatt
     5821 ggtttgtcat aaatcggcat caattgtttc gatgttgcct ttgttaaagg gtacagacgt
     5881 gttccgctgc ccccagcaag aatgattccc ttcatggata tctgctctcc tttttgattt
     5941 caaatttcac tttgttatta tctcatcaat tgttatattt gtcatggaaa tattaaggat
     6001 ttaaacatct tttatcaacg tttaaaccat tttcgtttga tcaacgccgt aatcagatga
     6061 acacgccaag aaattttttg ccgtaattta gaatgtgttt caatctcact cgcgtttaaa
     6121 tcatgtcttc ggtacagagt taacggttct cttaggaacc cagtcttctt tcctcccagc
     6181 aaaccaatcc acatatcatg catcggcact tctgacggga tcggcaaaat atcgggtttt
     6241 aaagaagacc ggaaagccat accagcacca atatagtgac ttcttatcac attattaagc
     6301 catccttctt tggaatttcg aaacgaaaaa tacgaatcat ggatctcttg cagctcgctg
     6361 tccacaatga ttaaatcaga aactactact agctgatccg gattctgctc aaaatagtca
     6421 gtgatctttt tcacttttgt ctccaaccaa acatcatctt ggtctgccaa aaagattaga
     6481 tctcctttcg tctgctggat cgcataacca aaattagcga tcaatccttg acgaggcccc
     6541 gaaaaaagct ggattcgctt atctttttct gcgtattcag ttaatatatc ccatgtatca
     6601 tctactgacc catcatcaga aatgactact tcatcttctg ctgaaagctg agataaaata
     6661 ctatctaact gctctcttaa atatctgcct ccattaaagg tagctataca aaccgaacgc
     6721 acgaattcac accttccaat tcttaacata tgagcgcaaa gtcatccgcc agatatgccg
     6781 gtttttgacg gtcaagaact gtttagctgt tcttaacaat aattgtcgtc ggtgactgga
     6841 taaatgcctt tgatcatatt catgataaaa tcgattttct gcttgtaaaa tagaacggta
     6901 tcgattactt gaaacttttt catagtctaa aaccgaaaga tcatgttcca ttcgtacagg
     6961 aaggatctcc acagtttttt ccaactgatc cactttccaa aacagccaat gatccaaaaa
     7021 atctaaagaa aaagcaggat taaacccgcc aatctttttt agaaatttga cagaccaggc
     7081 aacacctgaa ttgattgcca tgattcgttc agctgttggg ccttcaactt ctactgcttg
     7141 agctgtacga ttgatataac gatctgaata taggggagaa acttgtcggc tcccagaaaa
     7201 aatcatcgga actgcagcaa cacattcatc tgtaaaggtt actttacgca atagttcaaa
     7261 ataatcgtcg actagcctcg tatcctgatc cagagtgacg agacaacgtg ttccttcatc
     7321 tgcttgagac aacgcaaaat tataagccac cgctaaacca ggattttgcg gatcatgatg
     7381 ataaacgata ttttcttttt caaacagctc attttgctgc ctttgaggac tattatcgta
     7441 aataataagc tgaatttgct tttcgctaag tgctttgctt aatggtccat aagctggaga
     7501 ttgactgaat agttgctgat aaagcacaac gactactaat atttctggtt tcatcagaaa
     7561 aaacgccacc cccacttatt gaaaaatttc atcatagaag tcaaaaacac gcgaaacagt
     7621 tttgcatttc gatgcgcacc cttcccgtaa aaatggataa ctgcataaaa gggtgtgtac
     7681 aataacttgt aacctgcttt ttcaaaactc aaacacaagt cgttgtcttc aaagtacatg
     7741 aagaaacgct catcaaaccc ctttatctct ttaaatttat caatatcagc taccataaaa
     7801 ctacctgaga tcatccgtac ataactatta gcatcagtag gcaatcctcg gcattcaaac
     7861 acagccagtc gtttgtcaaa cttttttttg acccatttga aaggcacaaa gcgcaaaacg
     7921 taatcaaaaa cagccagctg ctcacggatg aggtgctgag ttgttccatc ttcattaaga
     7981 atcttaggtg ctaacatcgc acattccgga tgttctttca ataacaagac caatgctgta
     8041 actgtctctt catccactaa aatatctggg ttacaaataa tgccatagcg agaatcgcta
     8101 gctatcagat tgtgattatg tccataacca aatccggaat tttcatcatg aaaatagata
     8161 tcgatgtagg gatatttcct atactctgtt agtctttttt tgtactcggg aagtgaattg
     8221 ttgtcaaaaa ttcggatttt tactgctgtt tcatctttta cagttgaaat aaaattatct
     8281 agtgtccgaa aaattttatc actgtttgcc gtcacaatac taataaggat cggctggttt
     8341 aatttgttat tcatctatat gacggttcca tttcttcaaa tatttctgtt ttacttgcgt
     8401 acggtactct ttattcccta aaaatcgcaa tgtttttaaa ataaactgat gtttctcatt
     8461 gatcagacca atcaattcaa ataataaaat tgcagcaaac aacatggcaa taatcaatat
     8521 gatcgttccg attcttccag tataattcat gaccatagct gtgaaagaaa acatcaaagc
     8581 catcgcataa atcgtcagga cagcaccttt atgagtaaag cctaaactga ttagtcggtg
     8641 atgaagatgt tttttatctg ctgtagaaaa agaaacacgg tttgcttttc tcctaatgat
     8701 agcaaaaaac gtgtctgtta ccggcactcc catgacgatc agcaaagaaa tcgatgaaac
     8761 aaaagtcacg ttcttcagcc cttgtagtga aaggacggcc atcataaacc ctaggaatag
     8821 agctcctgta tcgcctaaat aaatctttgc cggataaaaa ttgtaaggaa aaaaaccgat
     8881 aatacttgct acaagacaga aaataacaac tggaatatac actgtagaag catggaggaa
     8941 aaaatatcca atgattccta tcgtaattaa accgatcaat gaaatccctg ccgccagccc
     9001 atccaaaccg tcaatcagat tgaccgcatt cgtgataccg aagatccaaa aaatcgttac
     9061 gggaaaacta agccagccta gttggatata tccaataaaa ggaagcgtga aggaatcaat
     9121 acgtatatct gctacaaaat aaataataag agaagcaatc aagatcccta tacttttttg
     9181 tttaggtgtc agctcatatt tatcatcgag cactcctgtg actatgatta ctaaagaagc
     9241 aatcaagatc ggtacaatat acgattgagg gataatttta cggaataaga ataaagtcga
     9301 tataacaaat gaaataaaga taggcagtcc gcccgctgaa ggcattggaa ctttatttat
     9361 tcttctttca tttggtttgt cgaccatacc tgtttgtact gaaatgattt tgaatacagg
     9421 tgttaatatc agtgataata aaattgtcag aaaaaatctt ataaccatac taaaagaaac
     9481 aagcatatgg gcacctactt taattaagag tcataattat tataaattat atatgtcttt
     9541 ttgactatct ttttatatct tctcaaatgt ccgcaaagcc ttattttatc ggctctacgg
     9601 gcattttgct tttgtggtaa acctcgcata tctaggtcta tcttcttata ttttccctgt
     9661 caatcgtggt taaaatcgtg gtaaatactt ctatgcgatt agacgctgaa cctctgattt
     9721 tgcggtatct atggacgcat gagcgtacca gttcattgtg atactgatgt ttgaatgccc
     9781 catgatatac tgtaaatctt tcgggttcat gttcttgctt gccagccttg tgcagaatgt
     9841 atggcgtagc gtatgcggtg taatatgtgg caagggattg tccttgtggt gcttgttgta
     9901 tttctttacc atacggataa ataaggcgtt ataatcaatg gcaacttttg gattaccttt
     9961 cggattgata aacacaaaac cgctgtgtcc gtctacctct atcgacttta tctttggtcg
    10021 gctgttgata acccgttgaa atgcctgtat cgtttctttg cttaatggca cttgccttat
    10081 cccgcttttc gtcttaggcg tttcaatata atagccctgt tccttactct ttagtaactg
    10141 gtggtcgata atcacaactt cattcttgaa atcaatgtcg gctactgtca gtccgcacag
    10201 ttccgagata cgaagtcctg tctttaacag tatcagcaca tcatcataat gcttgtgata
    10261 cacgttgtcc gtcttaataa atgacaataa ggcttgctcc tgttcctctg tcaatgcgac
    10321 tttctctttg gtatcatttt ctatgacttc acttagcttg aaatcaaaag ggtttttcct
    10381 cacataatca tcttgtatgg ctatgtagaa tgacgctttt aacgagcgtt tatggttgtt
    10441 aatggtattg taggaaaagc ctttgtcttt catgcgtaac gcccattctt tagcgtcaga
    10501 gggttttatc gtatcaatgc tcctagcacc taacttgtcc tcttttaata accgcatgag
    10561 ttgttcccgt tgtttctgtg tgcttttctt cacatttgtc ctctgtgcgt tctgtttggc
    10621 atagagttgg caaagcgtca ttttcttgcc tgtgctgtcg ataccgtcct caatatcccg
    10681 tcttatctgc tgttccagtt ctcgaagtga gggtttttcc cgttttccct ttggtgtcgg
    10741 gtctgtgggt gtcaatctcc aagcatacac atattttgtg tttcccaatg cgtctacata
    10801 tttatataag tattttccgt ctgttcgttg gctctctcca gtatgcagga tacgaccttt
    10861 gttatcccgt cttttttctt tcatggtgtc tgctcctttc cttgttggaa agagccttga
    10921 tatgacttgt gttcatcata acacatacaa ggctcatttg cattagattg cgtccaattt
    10981 atcaataacc ttttcaaact gtcggcgttt aatctgtatg cggttgccat tcataatgag
    11041 ccagccagcg tccttgtttt cctctgccaa tcgtcttaac ttgttttctc caatgcggaa
    11101 atactttgac gcttcttcaa tggtaagggt gtatttttcc catacgggaa tatcctgtgt
    11161 attactcata aaacaccccc tcactttcga ttacgtctga tatgtccgta cacatctgtt
    11221 ccacaagcat ttcctgtttt tcagaaaggt gggtacattc atcaatcatt ttcatgtagt
    11281 ttgaacctgt cagcctgtcg atttcctcta tcatcacgat tgacggcatg acctgtcttt
    11341 ccagccattg cagagtgcgt tccatattga caggtacagg gttcatttca aaaggtaaac
    11401 tgtcatggga aatgaatatc tcccacaatg gatagtcagg aaattccaca aaatcggtaa
    11461 tgagataaaa caccagccca tgagggttat acgtcaataa cagttcttca actgcctgta
    11521 ccgccttttt gtcacgcaga cgaatttcaa aacggttgat aatatcggta tgtttctttt
    11581 tcgttgcctg ttccttttgt ttttcataca ggcagaaata ttttgtgctt gcctttgagc
    11641 cgatataaag ggtacttgct aaattacggt tttttccgct taattttcca ccttgtacat
    11701 tttcatagtt tttacagcga cattccgcac caccatttct gtatttttca gatagcacag
    11761 gaatatccag cagaccgcac atatcattga ttgccaagtc aaaacgtcgg ataacaccgc
    11821 cacagtcaag acagcggttt aagaatgaat accagtccct gttctgtgcc tgtaagacat
    11881 attccatgtt gcgtgagcct gcccctttta attctaaaaa tacgcccatg tgttcatcat
    11941 cagaagccat gaccttgatt tccccgtaag cataatgctc cttatagcca taatacccat
    12001 agtcataatg gataaaataa tctgctttca tgccaagcac ctttcggata acttccaatg
    12061 cgtcagtcgt tggaaaacgg acagacagat aatcaacgag cagataaaaa ggcttatcgc
    12121 agatatagta attcagacaa cgcaggataa tgttctgtgt ttccttgctg gcgttagctt
    12181 ttccgttctc aaagcggttg atactatatc ttgatacatg ggtaagactt gcaagttctc
    12241 cttgtgaaag accgctggca agacgtttgt tcttcatttc aagtgcaaat tctaaattgt
    12301 tcattgtatt cctccaatcc cacaatagcc ttgaaatacc tgtaaaccac ctttcaaaaa
    12361 gtgtgatatg tactatcttc ttaaaacctc tcattactac tcacaggttt gggggtgtat
    12421 ggtttttgac ttgtcttttg tgaaatagtg cccccctgtt agataacggg ggcacagaca
    12481 aggctcgcag gctcgccagc cgacaaagca tttcttcccc ttgacacaag gtgtcatggc
    12541 ggtaagcctg agaggcttcc gccaccgtgt cagaaatact ttgtcagcaa gttctgtttt
    12601 gccttttcta acaagttcca tttcaaccga tattcatatc tacggattta atcagatagg
    12661 tatttctttt tgaatgtttt tatatttcat tctattgtgc ttattcgatt acaagaacat
    12721 agtaattctt atatgcttat ttgtcaatat ctttttttgc ctttacaaat catattaagc
    12781 atgatagact tataatagcg aagatataac gagatatgga gggttatgca atgaaaataa
    12841 gccatttagg aaataatata cagaccataa gaaaatttag gggaatgaaa caacaggaac
    12901 ttgcggacaa aatcggtatc aatatgcaga gcctttccaa gattgaaaga gggttgaact
    12961 atcctgccta tgaaacgctg gaaaagataa tggaagtgct ggacgttacg cccaatgaat
    13021 tgttgtcggg agaatggaag tatgtcaatc aatctgaaaa ggaagtctgt cagtttttaa
    13081 gagccgaaga acgtctaaat gcagaactga aacatggaca ctatgataac ttctttgaca
    13141 gcgaggaaga atggctggaa tatgagttag aaaagttacg ggaatatatt acagactata
    13201 tcaacgggaa aagcattgaa gcgtccgacc tttacccaat caaagaattt atccagcatt
    13261 tgaagtttca aaaattatta gaccgctatg atgatttata cagtatggat atgtttgggg
    13321 aaagcataga gggacataag tataagacac cttatcaagt cgtgaaaatg ataaacccga
    13381 actcaaaaga agatatggaa ctgttacgag aagtattaag gaataacaat tttgatgatg
    13441 aggacgagta acttttttcc tcttgcgaga agatacagca aacaaaaagc ccgatcaaga
    13501 gtgcaaagca ccaccaatgg tggcaaagct cttgacaggg ctttttcttt tctgttgtgg
    13561 gcaatcaaga ggaagaaagg gataaaatct gatttgtggt aaatcaggca aaggcaacgc
    13621 aggtatcaca gtatgaattg acaccgcata aggaaagcaa ctggaagatt gtagaataaa
    13681 tgaaaaaggc ttgcattctg catttttata aaattgcaag tctttatatt gacaagcata
    13741 aaatactaag atatacttta atcagaccaa cggtttgtat tggaggtaag ttatggcacg
    13801 aaataaatat ccagaagaaa cagtaaaact cattttagat gtttctacaa aaatgttttg
    13861 tgaaaaagga tatgataaca catcattgca agatattatc aatgaaacaa aactttctaa
    13921 aggtgctatt tatcatcatt tcgattcaaa ggaagatatt ttaaaagcaa tttttcaaag
    13981 aattggaaat gaaaatgcag taatatttgc taaaattaga gataacgata gtttaaacgg
    14041 tttagaaaag ttaagagaaa tatttaagac agcggtattc aattctaacc aaagcgtttt
    14101 attgacagtt tctcctcaac tattaaacaa tcctcgtttt cttgctatgc aaatagaaca
    14161 aatgtatgaa attgtagtac ctgaatttgt tgaacccgtt ttagaacaag gtatcaagga
    14221 taagtctttg gagattgaaa attcacatga aattgcagaa gttatcatgg tattaagtaa
    14281 tgtatggctc aacccattag ttgaaatgac ggataaagag ggtatggaaa aacgttgcaa
    14341 tacattcaat gcccttttaa atggaatagg catagacaca ttattagacg aagaaacaat
    14401 ttctgcattt atacagtttt gtaaataact gtatttgtta tccctatacg atagactgct
    14461 ctataaaaga gcagtttatt taaaccatta tataaaccga cggtctgttt gattaaataa
    14521 aaaggagaga ttttatgcaa gcagaaaaaa aactttttaa tcgtgatttt atacttgttg
    14581 ttataggaca gataatttct ttatttggaa acgctatcct acgatttgca ttaccgtact
    14641 atttgttaaa agaaactggt tcatcagctt tgtttggtat tgctacagcc tgctcttttt
    14701 tacctatggt tattttgtcg ctggtaggtg gtattattgc tgacaggaca aataaaagaa
    14761 acattatggt catactagat tttgtaacag caatcataat tttagtattt tatttgtcat
    14821 tgggaaaaat tcctatcata cctttgttta ttgttgcttt gatgttactt tatggtattt
    14881 ctggtgcata tcaaccagcg gtacaagcaa gtattcctct attggtatca tcagataaac
    14941 ttaccgtcgg taatgctata ataaatcaag taaatacttt agcaaacttt atcggaccag
    15001 ctataagcgg agtagttttt caattttatg gtatcaatcc cattttaatt gttagtatta
    15061 tatgttttac tttttcagcc attatggaaa tttttatcca tattccacac caaaaaagac
    15121 ctcgtgaagt aggtgtaatt gcagttgcta aaaacgattt aaaagatagt tatacctttt
    15181 tgaaaaagga gaaacccatt tttatatccg taatcttttt ggtttgcatt ttcaatttgg
    15241 ttttaagtgc tgttttaatt gttggtctgc ctatcttaat ttctgaagtt ttaaaaatgc
    15301 cagatacact atatggtttt tcacaatcag cattatcatt aggtggttta tgtggtggta
    15361 ttttaacagc tcttgttgct tcaaagttaa aactaaaaaa cctttatacc ttgttactta
    15421 tttgttctgt ttctgtttta cttatgggaa tttcgctatt tttagaattg cctgcaatag
    15481 tttcctactg ggtagttaca gtgatgtgct ttgtggctat gggtgtatct actatgtttg
    15541 ttgttcaaat ttacactctt gttcagacac aaacaccacc gcaattagta ggaaagataa
    15601 tggctacgct ggtagcagtt gctatgtgcg gtcaacctat tggacaaatg gtttacggta
    15661 ttttattcga tgtatttata caagaaacat gggttgttat gtttgtagct tctattgctt
    15721 caatcatgat ttcaatatat tcaaaaagaa tttttcaaaa gttagagata taatacaagg
    15781 ggttaaatag tatgttaggc taaaaacaat atataaaaga aaatttgaaa tctgctaaaa
    15841 ggatagtgtt ataaaagaaa gagtaatatt gcagtattta aggctcgcac aaatatatgt
    15901 tttgtgtggg cttttttctt accatttttc tgtatttcta aagggtataa acggtattcc
    15961 gtttatatat ttcagcatta gcagaaaggg ggtgagatta tgaggacatc ttctttcgag
    16021 catatcgtca gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa
    16081 agtcgaaaca gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc
    16141 gaaacgaaac aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt
    16201 aaagtcttgc actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga
    16261 ctgtcagaaa aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga
    16321 gaaatttcag aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga
    16381 ctaaggaagt taaaaatgct tttgagcgaa aggaagtaaa gaaatgagta aagattatgg
    16441 ctatccctct tttgaattga tttgcaaagc gtcaagcgga gatgaaatgg cagttaaaga
    16501 gattttgaag ttttatgacg gttatatttc taaattatgc ttgcgtcctt tttaccactc
    16561 tgaaagtggt aaaatcatta tgcaggttga tgaagaacta aaaggagaaa ttcatacaga
    16621 catgatgaaa gcaatattga aatttgaaat cggagtgaag taatcaactg tataaaaaaa
    16681 tcgggagata cacagtttcg tgctatctcc cttagtagtt ttacggtcat atcaaggctc
    16741 attcaatcgt ggtaaattcg tggtaagatg agtgtttttc tatacttcaa aatgcttgaa
    16801 tgtatagtgt ttatgcggat aattgaaaat tagatataaa atttcaaata aattactgat
    16861 ataaatttac aataagaact cttgtttcta ctttttttta tgagaaaatt tagttttcaa
    16921 tttat
//
LOCUS       DANKMN010000222        11945 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.guided.089, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000222 DANKMN010000000
VERSION     DANKMN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.guided.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            complement(125..1465)
                     /locus_tag="PW224_003006"
     CDS             complement(125..1465)
                     /locus_tag="PW224_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2909601.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1734..2630
                     /locus_tag="PW224_003007"
     CDS             1734..2630
                     /locus_tag="PW224_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2909602.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(2780..3721)
                     /locus_tag="PW224_003008"
     CDS             complement(2780..3721)
                     /locus_tag="PW224_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2909603.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            3847..4968
                     /locus_tag="PW224_003009"
     CDS             3847..4968
                     /locus_tag="PW224_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2909604.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5226..6728
                     /gene="eat(A)"
                     /locus_tag="PW224_003010"
     CDS             5226..6728
                     /gene="eat(A)"
                     /locus_tag="PW224_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2909605.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            6756..7322
                     /locus_tag="PW224_003011"
     CDS             6756..7322
                     /locus_tag="PW224_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2909606.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            7533..8594
                     /locus_tag="PW224_003012"
     CDS             7533..8594
                     /locus_tag="PW224_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2909607.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            8597..9493
                     /gene="murQ"
                     /locus_tag="PW224_003013"
     CDS             8597..9493
                     /gene="murQ"
                     /locus_tag="PW224_003013"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2909608.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            9507..11489
                     /locus_tag="PW224_003014"
     CDS             9507..11489
                     /locus_tag="PW224_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2909609.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11554..>11945)
                     /gene="nrdI"
                     /locus_tag="PW224_003015"
     CDS             complement(11554..>11945)
                     /gene="nrdI"
                     /locus_tag="PW224_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2909610.1"
                     /translation="AEYAEKMHAEDPVNPEVTLKEIHENSDFTKETEPFFTFVPTYLD
                     GGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGF
                     PFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3973 a         2163 c         2512 g         3297 t
ORIGIN      
        1 gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca agacttttgt
       61 cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa attttctatc
      121 gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg aaataaatga
      181 acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac aggataccaa
      241 ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc cataatgttc
      301 cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc aataatactg
      361 tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa taagaaagag
      421 ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt acttgatgct
      481 tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg aagaacaaaa
      541 ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg acaatcacaa
      601 aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc acaaaaggac
      661 tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa aacaaaggaa
      721 tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga atcgcacgtt
      781 taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg taagaataga
      841 taactacaag caaagaactg atgattccct tcataccatg aggagtaaac ccaccatggt
      901 cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga ccgacaagat
      961 aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc agccaatatt
     1021 ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa acagccaaca
     1081 taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag gaagcagctg
     1141 caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa tcagtaaaat
     1201 cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga tgcgtgtcgc
     1261 caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc agcccaccaa
     1321 tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca cttcctttaa
     1381 atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg gagctaagtt
     1441 gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca caatcaaaat
     1501 ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat tttcagacaa
     1561 ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt atttcatcat
     1621 attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact ggctttttac
     1681 tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa aagatgaaca
     1741 aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct tccatccgct
     1801 ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa tcagctagac
     1861 aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat atggagatgg
     1921 ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg aatcacttca
     1981 aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc gatgagattt
     2041 atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt tcattttatt
     2101 cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt ggtgcaacga
     2161 ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa agagatgtta
     2221 cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat cttacttatg
     2281 aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg cgggagggtc
     2341 agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa ctagtgacgg
     2401 ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt ttgcttcgag
     2461 aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca atcctatcag
     2521 agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact aacgattcca
     2581 ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa aaaacctaca
     2641 ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc atgagagttg
     2701 tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct gattcagctg
     2761 attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca tggactaatt
     2821 gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt cgccaaatgg
     2881 caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct tggtacatag
     2941 atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt tggtacactt
     3001 cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca gatacgattg
     3061 ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta caatccaaaa
     3121 tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg gaataacgaa
     3181 cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca actttttttg
     3241 taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca cctaacaatg
     3301 tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat aagatatttt
     3361 tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt aaacacaaaa
     3421 cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct aattgattgg
     3481 cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt tcttctggtg
     3541 aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc aacccatttg
     3601 tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca tttccgcttt
     3661 gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt gcgattttca
     3721 tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa tggaagaaaa
     3781 tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag aaagtgaggg
     3841 atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc ttcatttgaa
     3901 ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat caatgtgccg
     3961 attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag gggaacatgg
     4021 cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta tttattgaca
     4081 acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg gttagatcta
     4141 cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt aggagaactt
     4201 attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg aggaagcggg
     4261 tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga agggtacaca
     4321 gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa cgttcttgtt
     4381 tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg tcaacaagga
     4441 gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc tttcttagat
     4501 aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga tttagatgat
     4561 attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt gggagcgaag
     4621 atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa gatcgaacaa
     4681 gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca cggaaaagtt
     4741 ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc tatttgcgga
     4801 agtagagacc aagagctaga aggactttct gattttctcc caattgtctt aagtattcat
     4861 ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga aaaaacaaaa
     4921 atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt gacaaaatca
     4981 ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg tgcattgaaa
     5041 aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca aggaagcagt
     5101 gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg tgtgtatcca
     5161 acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt acgaggtgat
     5221 ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc caaggcacat
     5281 tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga cttatcggac
     5341 gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta ccttatcaag
     5401 ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa gataaagaac
     5461 gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa atcgaaagag
     5521 agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc acactatcgg
     5581 ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact catttcccgt
     5641 taatcgatga accaacgaat catttggata tctctggtag aaaacaagta gcggcttatt
     5701 tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt atcgatgaag
     5761 tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa gggaatttct
     5821 ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa aatgaaaaat
     5881 tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa tggtctcgtt
     5941 cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa tctagacgag
     6001 tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc aaagcaatcg
     6061 tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat atcgaatata
     6121 tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta agcgtagaag
     6181 atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca atcgagcctc
     6241 atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt atccattatc
     6301 ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa catctgagca
     6361 ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa tttgcagaga
     6421 aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg atggaaagag
     6481 atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa gtggaattgg
     6541 ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg aattatttgg
     6601 atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct gccatgttac
     6661 tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt tctcttgaga
     6721 gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac tttgccaacg
     6781 aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg aacgcacaag
     6841 gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg caggcacaga
     6901 aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc cattcccctc
     6961 ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg ccgctgaccg
     7021 ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca agcaaagatg
     7081 agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg gagtcagtct
     7141 tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac gaagaaaatg
     7201 tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat tttcatccga
     7261 tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt tcttatgagt
     7321 aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg cttagcagtg
     7381 ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag aaatgaaatt
     7441 taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc tagtcataga
     7501 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
     7561 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
     7621 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
     7681 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
     7741 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
     7801 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
     7861 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
     7921 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
     7981 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
     8041 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
     8101 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
     8161 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
     8221 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
     8281 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
     8341 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
     8401 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
     8461 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     8521 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     8581 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     8641 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     8701 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     8761 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     8821 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     8881 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     8941 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     9001 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     9061 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     9121 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     9181 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     9241 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     9301 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     9361 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     9421 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     9481 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     9541 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     9601 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     9661 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     9721 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     9781 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     9841 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     9901 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     9961 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
    10021 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
    10081 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
    10141 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
    10201 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
    10261 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
    10321 tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct
    10381 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
    10441 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
    10501 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
    10561 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
    10621 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
    10681 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
    10741 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
    10801 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
    10861 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
    10921 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
    10981 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
    11041 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
    11101 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
    11161 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
    11221 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
    11281 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
    11341 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
    11401 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
    11461 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
    11521 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
    11581 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
    11641 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
    11701 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
    11761 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
    11821 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
    11881 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
    11941 tgcta
//
LOCUS       DANKMN010000223         7958 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.guided.101, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000223 DANKMN010000000
VERSION     DANKMN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.guided.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            128..1564
                     /locus_tag="PW224_003016"
     CDS             128..1564
                     /locus_tag="PW224_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2909611.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="PW224_003017"
     CDS             1753..4200
                     /locus_tag="PW224_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2909612.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="PW224_003018"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="PW224_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2909613.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="PW224_003019"
     CDS             complement(5690..6793)
                     /locus_tag="PW224_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2909614.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7345..7893
                     /gene="aac(6')"
                     /locus_tag="PW224_003020"
     CDS             7345..7893
                     /gene="aac(6')"
                     /locus_tag="PW224_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2909615.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2205 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taatttttta ttttataagt aagataaata atgaaagaat agagatactg
     7261 attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat
     7321 aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta
     7381 ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc
     7441 tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac
     7501 caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa
     7561 ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc
     7621 aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat
     7681 gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa
     7741 gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat
     7801 aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca
     7861 aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc
     7921 agcttcaaga aataagaagg aatttacgaa aattactt
//
LOCUS       DANKMN010000224         5008 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.guided.121, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000224 DANKMN010000000
VERSION     DANKMN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.guided.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            5..604
                     /locus_tag="PW224_003021"
     CDS             5..604
                     /locus_tag="PW224_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2909616.1"
                     /translation="MTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSSVNMQPWRFVV
                     AESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAYESGKMPKEVR
                     DQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPIGGVEADQLAE
                     AFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            958..2436
                     /gene="msr(C)"
                     /locus_tag="PW224_003022"
     CDS             958..2436
                     /gene="msr(C)"
                     /locus_tag="PW224_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2909617.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2483..2929
                     /locus_tag="PW224_003023"
     CDS             2483..2929
                     /locus_tag="PW224_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2909618.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3128..3643)
                     /locus_tag="PW224_003024"
     CDS             complement(3128..3643)
                     /locus_tag="PW224_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2909619.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3760..4962
                     /locus_tag="PW224_003025"
     CDS             3760..4962
                     /locus_tag="PW224_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2909620.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1524 a          842 c         1089 g         1553 t
ORIGIN      
        1 aattatgact ggtagaagat ccgttcgcgt atatgatgaa acatacaaaa tcccacacga
       61 agaaatgctg gaaatgatca aagaagcgac gactgcacct tcttctgtca atatgcaacc
      121 atggcgcttt gtggtcgctg aaagcgagga agcaaaagaa atcttgcgtc ctttgatccg
      181 tttcaatacg agacaaaatg atacttcttc tgcaatggtc atgatttttg gcgatatgca
      241 atgttacgaa tatggcgaag aaatctataa ccaagcttac gaatcaggga agatgccaaa
      301 agaagtcaga gatcaacaat tagctgcgat cattccttat tacaagagtt tttcaagaga
      361 agaaatgaac gatgtagtca aagtcgattc tagcttagcg gctatgcaat tcatgttagt
      421 tgcgcgtgat catggctatg aaacaaatcc aattggtgga gtcgaagcag atcagttggc
      481 agaggcattt ggtttggata aagaccgtta tgttccagtt attattttat caataggaaa
      541 agcggtagaa gaaggttacg agtctgttcg tttagcacca gaaaagatca ctacgttcga
      601 ataaaaggtt ttcagttact attcagtagc tattgctttt tttgaatcgt gtgtggtaac
      661 ctaataaaaa aggaggtcgg aaaaatgact gcatcgatga aattacgttt cgaacttttg
      721 aataacaact aaataaattc aaaagacatg tataaacaac aatttcatgg atggagtatg
      781 tccatttttt cgaacgacag tagaataggt gaaagctatg acagatttgg tgatttagtc
      841 atagttattt cgttcagagt aggaaaagat gtgatgcagt ttttttgcaa acagattttt
      901 ctgctctttt ttagtatcga taattgttgt tttacatgaa ataggagtgt aaaacatatg
      961 gaaaatttag cagtaaatat aacaaatcta caagtcagct ttggcaacca gctagaacta
     1021 tctattgatt ctcttcgtgt ctatcagcaa gatcgtatag ggatcatagg agaaaatggt
     1081 gtcggtaaat cgactttgct caaactaata gccggtgaac tttttcccga tcatgggaaa
     1141 atccaaacag agatcacctt caactacctg cctcaattaa cctatctcgc tgaagcaaag
     1201 gacctaaatt tggaattagc cagtcatttc cagttaagac tggaagaaac ttcagagcgg
     1261 aaatggagcg gaggggaaga acgaaagatc gagttgatac gtcttctttc ttcttatgaa
     1321 caagggatgc ttctagacga gccaacaacc catctagata gaaaaagtat tgatcgactg
     1381 attgaagagc ttcgttatta ttatggtacg ctggtttttg ttagtcatga tcgctatttt
     1441 ctagatgagc tagcatcgaa aatctgggaa gtaaaagacg gagaaatccg agagttttcg
     1501 gggaattata gtgcctatct cactcaaaag gaattggaga aaaagactca gctacgagaa
     1561 gcagagtcga tcatgaaaga gaaaaaacga ttggaaaaat cgatccaaga aaagaaaaaa
     1621 caagcggaaa agttggaaaa agtgtccagt aaaaagaaaa agcaacaaat cagaccggat
     1681 cggttgtctt cctctaaaca aaaagacagt gtacaaaaag ccatccaaaa gaatgcgaaa
     1741 acattagaga gaagactcca aaaaatagga gaaacaacca agccgcagca gatgaaacaa
     1801 atccgttttc cagtaccaaa atctcttgaa ctccacagcc gctatcccat catgggacaa
     1861 aatgtccaat tggaaagaag tgggagaaca ttactggtaa atggtgattt tcagttttct
     1921 ttaggtaaaa aaatcgcgat tgtcggtgaa aatggttcag gtaagacaac cttattagaa
     1981 catatccgca aacaaggaga aggaatcctt ctctctccga aagtaagctt tcaagtatat
     2041 cagcaaaagg gttatcaaat gacatctgaa gaatccatca ttcgttttgt catgagacaa
     2101 acagagtttt cagaatcgct tgtccgtagt ttgctgaatc acttagggtt tgctcaggaa
     2161 actttgacga aaccgttatg tacattaagt gggggagaag cgacccgtct gacgattgct
     2221 ttgcttttta ctaagccaag taatgtgttg ctgttagatg aaccgactaa ttttattgat
     2281 atggcaacga tcgaagcttt agagaagcta atgcaaatat atccgggaac gattttgttt
     2341 acttctcatg attcttactt tgtcgagcgt acggctgatg aagtttatga aataaaaggg
     2401 cagaaaataa aaaaagtact tacgagaaat ttttaaatgt gctacgatga ttttcgtgac
     2461 aaatttaaag gagtggtaca gattgcctac aaataaaaaa gaaggaataa tttttacaac
     2521 aatgatgtgt tttttgatgg tcttggggat gagcgcgtat aatttatggc tgcatcaaga
     2581 tcttcattta tcaagtttgt tgattggtct agtgcctgga ttcgtggtgg ctttcatttt
     2641 ggatgtcttc atcatcggtg tgctggcgaa aaaaattgcc tttaaactac cagtaaatcc
     2701 agaaagcaag gtccaaatga tcttaactat ttcatgcttg atggttttag gaatggtgac
     2761 atgtatgtct ttattcggtg tgatcatgga acaaggaata cctgatcaat tattctctgc
     2821 ttatatgaat gcttggaaaa tgaatgtgat tgccgcttta ccgttgcagt tgatcatcgt
     2881 tggcccgctt tcaagaagtg tgctaaaagc aattcaaagt aatgaatgat ggaacactgt
     2941 tatttgaaaa aaggccttgg catgcttttt gtcatagtct cttgaatgca gttctagaaa
     3001 aatagtgatc cgaacgatag gttttggcct gtcgttcgga tcgctttttc ttttccaact
     3061 gtattgctct tttattgaag ataatttgct cttcaataga tatttctaga aaggttctgt
     3121 ttatccctta ctcaaaaaac tgctgcattt gttctcttac ttgctgacac cattggagtt
     3181 cctgctgaca aagcataatt ctgcgctgga tcacaacata acgtccaaat tcttgtttct
     3241 tgtctagcga gaggtcattc aatttttttt gatttttttg catgattttg ttcagctgtt
     3301 gttctctagt gaatagcagt ccagtagctg ttgttttatc gagcgtcgaa aaagcataga
     3361 tttttgctag aaattcatct ttttgcgtag gagcgggagt ttcctctttg atccattcct
     3421 ctacaagagc acgtccttca tcagtcaagt ggtagatctt tttttgagag tgctcgtctt
     3481 tgtccaaaac ggtcacataa ccctgctggt ctaacttggc aagcgttgta tagatctgac
     3541 tgtgatgggc ttcccaaaaa attgtgatca gctgcttcaa ttcataccca gaacgtggtt
     3601 tggataaaag cacgcataat aatatatagg ctaaagtatt cataaaaatc ctacctctcg
     3661 tcttattttc tttattatat cacagtaaag tttccacgtt ttacatgtgc atatttacat
     3721 gtaaaataaa tcatgtatga tgctaataga gagaaaggga tggaaatatt ggagaatcaa
     3781 atagatatac aagaacaaac aaatgaagaa acaaagggga aaagttcagc gctagttgta
     3841 gcagctacct gtatgatcgc ttttatggga gtgggtctag tagacccaat cttaaaaacg
     3901 attgctgcac agttaaacgc tacgccagct gaaacgaccc tgttatttac tagttacatg
     3961 ttagtcactg gtgtggtgat gctatttaca ggtttcttat ctagtcggat cggtttgaaa
     4021 aaaacattga tgacaggatt gtttatcatc gttgtattcg ctggtcttgg cggattatct
     4081 gggaatatcg gaatgctgat tgggttacgt gccggctggg gtgtcgggaa tgctttattt
     4141 gtctctactg ctttagcaac gatcgtcagt attttagttg gtaacacaga aaaagcaatc
     4201 atgatgtatg aagcagcact tggctgcgga atggctgttg gccctctagt cggtggggtc
     4261 ttgggcagtg gttcatggcg ttatccattt tttggggtag catttttgat gttttgcggt
     4321 tttatagccc tagcggtact tttacctgaa actgagaaac caaagagaaa agtaggcctt
     4381 tgggaaggtg tcaaagcgtt aagcaatcat aaacttcgtt cagttggact aatcgcattg
     4441 ttatataatt tcggattctt tactttactt gcgtatgcgc catttttatt gattggctat
     4501 tctgaattag aagtcggttt tgtatttttt ggctggggtg tgcttctggc tattgcttcg
     4561 attttcgttg caccggctct tgagcggaag tttacgactt atcggacaat tttagcagca
     4621 tttgtcttat tcattatttg tttgctgtta ttaggagctg gaacttctat gccagtcctt
     4681 gtaccgatca gcattgtgtt agcaggattt ttccaaggaa ttatcaatac actgttgacg
     4741 acgattgcta tggaaatacc aggtctgcaa cgaaacgttg cttcgtccag ctatagtttt
     4801 gtccgctttt tcggtggagc tttagcacca tttattgcag gtgtgattgg tgaacatatt
     4861 ggtgaaagct atgcgtttta ttttgcaggt gccattgttc ttatcagtgt atgttgtatc
     4921 atttatcacc gtaattattt tgctacaaat gaagaagaat aagaaatcag aggttgtgaa
     4981 aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DANKMN010000225         3079 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.guided.146, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000225 DANKMN010000000
VERSION     DANKMN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.guided.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..1269
                     /locus_tag="PW224_003026"
                     /note="aph(2'')-Ia"
     CDS             <1..1269
                     /locus_tag="PW224_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HDL2909621.1"
                     /translation="VFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQF
                     IGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDL
                     PEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAY
                     LVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYK
                     EIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYI
                     LLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGI
                     IDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYP
                     IETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1399..2571)
                     /locus_tag="PW224_003027"
     CDS             complement(1399..2571)
                     /locus_tag="PW224_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDL2909622.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         1066 a          400 c          553 g         1060 t
ORIGIN      
        1 gtttttagag taattattga atataacaat gttcctattg gatatggaca aatatataaa
       61 atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat agtctatggt
      121 atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac aagatatatt
      181 aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat tttagaccct
      241 cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag aattattgaa
      301 gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt aatggaatat
      361 agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga gcattacttt
      421 gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag tgtggcatat
      481 ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa aaaaggttat
      541 gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa tgtaaaaatt
      601 cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta taaagaaatt
      661 aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga acaaaatttg
      721 ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta tacagatatt
      781 agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt gttgcgtgaa
      841 actatttata atgatttaac tgatatagaa aaagattata tagaaagttt tatggaaaga
      901 ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga ttttagttgt
      961 aatcatctat tgttagatgg caataataga ttaactggaa taattgattt tggagattct
     1021 ggaattatag atgaatattg tgattttata tacttacttg aagatagtga agaagaaata
     1081 ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat tgagaaagca
     1141 aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt ttatggaatt
     1201 aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa aaggacttat
     1261 aaagattgat tatataatat atgaaaagct attataaaag acattagtat taaatagttt
     1321 aaaaaaatga aaaataataa aggaagtgag tcaagtccag actcctgtgt aaaatgctat
     1381 acaatgtttt taccatttct acttatcaaa attgatgtat tttcttgaag aataaatcca
     1441 ttcatcatgt aggtccataa gaacggctcc aattaagcga ttggctgatg tttgattggg
     1501 gaagatgcga ataatctttt ctcttctgcg tacttcttga ttcagtcgtt caattagatt
     1561 ggtactcttt agtcgattgt gggaatttcc ttgtacggta tattgaaagg cgtcttcgaa
     1621 tccatcatcc aatgatgcgc aagcttttga atattttggt tgatcgatat aatcatgaat
     1681 caatcgattt ttagcctcac gcgctaagtt aatatctgtg aacttaaaaa ttcctttaac
     1741 agcttctctg aaagattttg aatttttttt aggaatggtg gtaaagatat ttcttaggaa
     1801 gtgaacttgg catctttgcc aacttacgtt ggtgaaggat tttctaatgg cagagactaa
     1861 tcctttgtgc gcatcagaaa taacgagttc cgtaccttgt aaaccgcgtt cttttaggta
     1921 ttcaaaaaat gttgtccagg tctcttcgct ttcgccactt tgaatcatga agccgataat
     1981 ttcacggtcg ccatctttgg ttattccaat cgctatatga cagctttttg agagtactcg
     2041 attttcttct cgtactttta tatagagtac atcggtcatt aagtaaggat aatttttttc
     2101 tgataataaa cgattctgcc actcgttaac cataggttct agctgttctg ttaagctaga
     2161 aacgaaggac ttagagacgg atttaccaca aagttcttcc acaatttttg atactttacg
     2221 agttgaaacg cctgatacat acatttccaa cattgaagcc atgagggctt tttcgtttcg
     2281 ttgataacgt tcaaacactg tgggtgaaaa atggccatca cgtgttctgg gtacttttaa
     2341 ttctagcgtg cctacacgtg tcgtaaagct gcgctcataa tagccatttc gttgactttg
     2401 tcggttttct gttcgttcat attcttttgc ttgaatatat tctgttcgtt gattttccat
     2461 tagttgatta aataccgttg ttaaaatatt tttagaaacg tcatccttta cagaatattc
     2521 aataatgctt tgaatctctt cgcttttcag tgtaaaatgt acttgggtca tgtaaaagtc
     2581 ctcctgggta tgtttttgtc gttaaaaaca ttgtaccgta aaaggactgt tatatggcct
     2641 ttttactttt acacaattat acggacttta tctatataaa ggcgctgtca gagttttttg
     2701 acttagaaca tacattcatt caagagactt taaacaagct agcagaacgc ccaaaaacgg
     2761 acacacaact cgatttgttt agctatgata caggctgaaa ataaaacccg cactatgcca
     2821 ttacatttat atctatgata cgtgtttgtt tttctgtact gtttcctgag tgattagcag
     2881 aaatataaag attaaaattt taattgatta ttaggaagag aaggagaatg tatcccgaaa
     2941 actttttagt tggcttggac tgaacgaagt gagggaaagg ctactaaaac gtcgagggga
     3001 gtgggagcga agcgcacact tgatcttttg aacttttttc ggttctgttg caaagtttta
     3061 aatctactat caaataagg
//
LOCUS       DANKMN010000226         1781 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.guided.166, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000226 DANKMN010000000
VERSION     DANKMN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.guided.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            24..416
                     /locus_tag="PW224_003028"
     CDS             24..416
                     /locus_tag="PW224_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2909623.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="PW224_003029"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="PW224_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDL2909624.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DANKMN010000227         1037 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538704-rid17234983.guided.186, whole genome shotgun
            sequence.
ACCESSION   DANKMN010000227 DANKMN010000000
VERSION     DANKMN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538704
            Sequence Read Archive: ERR10688267
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638034.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638034.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:43:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 35 of 62
            Pseudo Genes (incomplete)         :: 25 of 62
            Pseudo Genes (internal stop)      :: 13 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538704-rid17234983.guided.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-03-26"
     gene            <1..109
                     /locus_tag="PW224_003030"
     CDS             <1..109
                     /locus_tag="PW224_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2909625.1"
                     /translation="NSSLKQYIQKLEKAKDEVEDTADKSQQIVEEHLRK"
     gene            436..933
                     /gene="dfrG"
                     /locus_tag="PW224_003031"
     CDS             436..933
                     /gene="dfrG"
                     /locus_tag="PW224_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2909626.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          414 a          115 c          192 g          316 t
ORIGIN      
        1 aaactcaagc ttgaaacaat atattcaaaa gttagaaaaa gcaaaagatg aagtggaaga
       61 tacagctgat aaatctcagc aaattgttga agaacatttg cgtaaataat ctttcttaac
      121 aagtaaacct aaattagtaa agaagcaaca gtaatctata ctaagtagtt cagttttgga
      181 gtacaaaatg acaaaatgta cttcaaaatg gactactttt ttcattttaa cttacatacc
      241 aaattacata caaaatgatt tataaaatgc aattgaaaat gataattgaa tgaaaatttg
      301 tgattttgtg gtattatgat aatataactt atagaaaaaa ttgcttattc aactaacggg
      361 caggataatg agaaaaggtt ttttgataga aataagaaat tagatggtaa attagttaaa
      421 aaggctgtgt tataaatgaa agtttctttg attgctgcga tggataagaa tagagtgatt
      481 ggcaaagaga atgacattcc ttggaggatt cccaaggact gggaatatgt taaaaatact
      541 acaaagggac atccgataat attaggtagg aagaaccttg aatcaatcgg aagagcctta
      601 cctgacagaa gaaatattat tctgacgaga gataaggggt ttacctttaa tggttgtgaa
      661 attgttcatt caatagaaga tgtttttgag ttatgtaaaa acgaagaaga aatttttatt
      721 ttcggaggag aacagattta taatttgttt ttcccttatg ttgagaaaat gtacatcaca
      781 aaaatacatc atgaattcga aggagatact ttttttccag aagtgaatta tgaggaatgg
      841 aatgaggtat ttgcccaaaa agggataaag aatgataaaa atccgtataa ctactatttt
      901 catgtatatg aaagaaaaaa cttattgagt taacgggtgc gttaattgaa gaagaacagt
      961 aaagataaag cacaagagct tgggattact ccaagctctt tttgtcattt gaaataaatt
     1021 atatctttcc aatcaaa
//
